AT1G67700

metalloendopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011089
Physical Location & Seq
Forward (+)
25374072 .. 25376085
2014 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G67700.5

Sequence Viewer

Length: 693 bp
ATGGAAGTGAGTATGTCTTTGAATGCGTTAACTCGGCTTCCTCTGAAGAACACAGGAAGATTCGAAGAGGTTGGTTTAGCGAGACACTCTCTGTTTAGCTCAAGAACGGCATGCCGAGAGACGGCGGTACAGCAGCGGCGGATGGTTTTTGTGGTGGAGGCTAAGGGAAAGAAAGGTATGGCGGCGCGTCAGTATCAACGTACACCTCCTCCTATGCCTAAGATTGAAGACGATGGAAACCCAAGATTCGTTATCTTCATTCGTATGGCCAATGTGTATCTTTGGTATCCTCTGAGTATAATAGCTGGTGGTACTACTGCTAAGATCATGGTTGCTGCAAAAGATAACCTCTTGGGGAAGTATATCTACAAAGACACCATTGCAAGAAACATTGCCGCTGTTATTTACAGAGATGAGAAAGAGATTCAAAAGACAGCAATTAAGCAGCATCGTGTCTTGCGTACAGCCACCGAGTTTCGATATGGCTACAAACTTGTAGAAAACGGAAACATGAGAGCTGCACTTAGTACTTCAGATGTTATTGAGCTCCCAACCCAGGACCAGCTTAAAACAGTGTTCGACAAAGTTAAAGACTACTTTGGGGATGCAAAAGAGTCATTCGGAAAGCTATCATCGCTGAACCCGGGGAGTGACGAAAAAACTGAAGAAACCTCGGATGAGAAAGCCAAGTAA

Protein Analysis

230

Amino Acids

26.01

Weight (kDa)

9.57

Isoelectric Point (pI)

33.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HHL1-like PF20133 55 - 200 7.4e-11 Protein HHL1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011089)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67700 AT1G67700 AT1G67700 AT1G67700 AT1G67700
fragaria_vesca FvH4_4g24830
malus_domestica MD13G1108600.v1.1
prunus_persica Prupe.1G237600_v2.0.a1
pyrus_communis pycom13g09410
rosa_chinensis RchiOBHm_Chr4g0431911
rosa_laevigata RLG00000006876
rosa_multiflora Rmu_sc0004965.1_g000007
rosa_roxburghii Rroxscaffold_5G00373340
rosa_rugosa Rorug04G0253000 Rorug04G0253000
rosa_samantha Rh4AG310200 Rh4BG317300 Rh4CG332600 Rh4DG312200
rosa_wichuraiana Rw4G026990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 187
AciI CCGC 5 cut(s) 125, 136, 139, 182, 396
AcoI YGGCCR 1 cut(s) 267
AcuI CTGAAG 3 cut(s) 65, 516, 684
AfaI GTAC 5 cut(s) 129, 202, 313, 463, 529
AfiI CCNNNNNNNGG 2 cut(s) 121, 556
AgsI TTSAA 3 cut(s) 22, 227, 428
AjnI CCWGG 1 cut(s) 555
AluBI AGCT 6 cut(s) 99, 305, 518, 547, 565, 628
AluI AGCT 6 cut(s) 99, 305, 518, 547, 565, 628
Alw21I GWGCWC 1 cut(s) 549
Alw26I GTCTC 2 cut(s) 76, 113
Ama87I CYCGRG 1 cut(s) 643
AoxI GGCC 1 cut(s) 267
ApeKI GCWGC 4 cut(s) 133, 335, 445, 518
AspLEI GCGC 1 cut(s) 187
AspS9I GGNCC 1 cut(s) 559
AsuC2I CCSGG 2 cut(s) 644, 645
AsuII TTCGAA 1 cut(s) 63
AvaI CYCGRG 1 cut(s) 643
AvaII GGWCC 1 cut(s) 559
BalI TGGCCA 1 cut(s) 269
BanII GRGCYC 1 cut(s) 549
BarI GAAGNNNNNNTAC 2 cut(s) 350, 382
BbsI GAAGAC 1 cut(s) 234
Bbv12I GWGCWC 1 cut(s) 549
BbvI GCAGC 4 cut(s) 145, 322, 457, 505
BccI CCATC 2 cut(s) 136, 227
BceAI ACGGC 2 cut(s) 123, 138
BciT130I CCWGG 1 cut(s) 557
BciVI GTATCC 1 cut(s) 297
BcnI CCSGG 2 cut(s) 644, 645
BcoDI GTCTC 2 cut(s) 76, 113
BfuI GTATCC 1 cut(s) 297
BisI GCNGC 7 cut(s) 134, 137, 183, 336, 396, 446, 519
BlsI GCNGC 7 cut(s) 135, 138, 184, 337, 397, 447, 520
BmcAI AGTACT 1 cut(s) 529
Bme1390I CCNGG 3 cut(s) 557, 644, 645
Bme18I GGWCC 1 cut(s) 559
BmeT110I CYCGRG 1 cut(s) 643
BmgT120I GGNCC 1 cut(s) 559
BmrFI CCNGG 3 cut(s) 557, 644, 645
BmsI GCATC 2 cut(s) 457, 595
BpiI GAAGAC 1 cut(s) 234
BplI GAGNNNNNCTC 2 cut(s) 73, 105
Bpu10I CCTNAGC 1 cut(s) 162
Bpu14I TTCGAA 1 cut(s) 63
BpuEI CTTGAG 1 cut(s) 85
BpuMI CCSGG 2 cut(s) 644, 645
BsaJI CCNNGG 4 cut(s) 555, 643, 644, 672
BsaXI ACNNNNNCTCC 2 cut(s) 193, 223
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 556
Bse3DI GCAATG 2 cut(s) 378, 390
BseBI CCWGG 1 cut(s) 557
BseDI CCNNGG 4 cut(s) 555, 643, 644, 672
BseGI GGATG 3 cut(s) 147, 610, 682
BseLI CCNNNNNNNGG 2 cut(s) 121, 556
BseMI GCAATG 2 cut(s) 378, 390
BseMII CTCAG 1 cut(s) 284
BseRI GAGGAG 1 cut(s) 198
BseXI GCAGC 4 cut(s) 145, 322, 457, 505
BsgI GTGCAG 1 cut(s) 504
Bsh1236I CGCG 1 cut(s) 187
BshFI GGCC 1 cut(s) 269
BsiHKAI GWGCWC 1 cut(s) 549
BsiHKCI CYCGRG 1 cut(s) 643
BsiSI CCGG 1 cut(s) 644
BslI CCNNNNNNNGG 2 cut(s) 121, 556
BsmAI GTCTC 2 cut(s) 76, 113
BsmBI CGTCTC 1 cut(s) 113
BsmI GAATGC 1 cut(s) 28
BsnI GGCC 1 cut(s) 269
BsoBI CYCGRG 1 cut(s) 643
Bsp119I TTCGAA 1 cut(s) 63
Bsp1286I GDGCHC 1 cut(s) 549
Bsp143I GATC 1 cut(s) 324
BspACI CCGC 5 cut(s) 125, 136, 139, 182, 396
BspANI GGCC 1 cut(s) 269
BspCNI CTCAG 1 cut(s) 285
BspFNI CGCG 1 cut(s) 187
BspT104I TTCGAA 1 cut(s) 63
BsrDI GCAATG 2 cut(s) 378, 390
BssECI CCNNGG 4 cut(s) 555, 643, 644, 672
BssMI GATC 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 557
Bst4CI ACNGT 1 cut(s) 574
Bst6I CTCTTC 1 cut(s) 60
BstBI TTCGAA 1 cut(s) 63
BstC8I GCNNGC 1 cut(s) 112
BstDEI CTNAG 5 cut(s) 162, 219, 293, 321, 524
BstF5I GGATG 3 cut(s) 147, 610, 682
BstFNI CGCG 1 cut(s) 187
BstHHI GCGC 1 cut(s) 187
BstKTI GATC 1 cut(s) 327
BstMAI GTCTC 2 cut(s) 76, 113
BstMBI GATC 1 cut(s) 324
BstMWI GCNNNNNNNGC 1 cut(s) 634
BstNI CCWGG 1 cut(s) 557
BstNSI RCATGY 1 cut(s) 114
BstSCI CCNGG 3 cut(s) 555, 642, 643
BstUI CGCG 1 cut(s) 187
BstV1I GCAGC 4 cut(s) 145, 322, 457, 505
BstV2I GAAGAC 1 cut(s) 234
BsuI GTATCC 1 cut(s) 297
BsuRI GGCC 1 cut(s) 269
BtgZI GCGATG 1 cut(s) 618
BtsCI GGATG 3 cut(s) 147, 610, 682
BtsIMutI CAGTG 1 cut(s) 579
Cac8I GCNNGC 1 cut(s) 112
CfoI GCGC 1 cut(s) 187
Cfr13I GGNCC 1 cut(s) 559
Cfr9I CCCGGG 1 cut(s) 643
CseI GACGC 1 cut(s) 176
Csp6I GTAC 5 cut(s) 128, 201, 312, 462, 528
CviAII CATG 3 cut(s) 111, 328, 511
CviQI GTAC 5 cut(s) 128, 201, 312, 462, 528
DdeI CTNAG 5 cut(s) 162, 219, 293, 321, 524
DpnI GATC 1 cut(s) 326
DpnII GATC 1 cut(s) 324
EaeI YGGCCR 1 cut(s) 267
Eam1104I CTCTTC 1 cut(s) 60
EarI CTCTTC 1 cut(s) 60
EciI GGCGGA 1 cut(s) 154
Ecl136II GAGCTC 1 cut(s) 547
Eco24I GRGCYC 1 cut(s) 549
Eco47I GGWCC 1 cut(s) 559
Eco53kI GAGCTC 1 cut(s) 547
Eco57I CTGAAG 3 cut(s) 65, 516, 684
Eco88I CYCGRG 1 cut(s) 643
EcoICRI GAGCTC 1 cut(s) 547
EcoRII CCWGG 1 cut(s) 555
EcoT38I GRGCYC 1 cut(s) 549
Esp3I CGTCTC 1 cut(s) 113
FaeI CATG 3 cut(s) 114, 331, 514
FatI CATG 3 cut(s) 110, 327, 510
Fnu4HI GCNGC 7 cut(s) 134, 137, 183, 336, 396, 446, 519
FokI GGATG 3 cut(s) 154, 617, 689
FriOI GRGCYC 1 cut(s) 549
Fsp4HI GCNGC 7 cut(s) 134, 137, 183, 336, 396, 446, 519
GlaI GCGC 1 cut(s) 186
GluI GCNGC 7 cut(s) 134, 137, 183, 336, 396, 446, 519
HaeIII GGCC 1 cut(s) 269
HapII CCGG 1 cut(s) 644
HgaI GACGC 1 cut(s) 176
HhaI GCGC 1 cut(s) 187
Hin1II CATG 3 cut(s) 114, 331, 514
Hin6I GCGC 1 cut(s) 185
HinP1I GCGC 1 cut(s) 185
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HinfI GANTC 4 cut(s) 60, 246, 424, 614
HpaI GTTAAC 1 cut(s) 30
HpaII CCGG 1 cut(s) 644
Hpy166II GTNNAC 2 cut(s) 30, 203
Hpy188I TCNGA 5 cut(s) 45, 294, 535, 623, 676
Hpy188III TCNNGA 1 cut(s) 102
Hpy8I GTNNAC 2 cut(s) 30, 203
HpyCH4III ACNGT 1 cut(s) 574
HpyCH4IV ACGT 1 cut(s) 199
HpyCH4V TGCA 4 cut(s) 338, 383, 521, 608
HpyF10VI GCNNNNNNNGC 1 cut(s) 634
HpyF3I CTNAG 5 cut(s) 162, 219, 293, 321, 524
HpySE526I ACGT 1 cut(s) 199
Hsp92II CATG 3 cut(s) 114, 331, 514
HspAI GCGC 1 cut(s) 185
KspAI GTTAAC 1 cut(s) 30
Kzo9I GATC 1 cut(s) 324
LmnI GCTCC 1 cut(s) 552
LpnPI CCDG 6 cut(s) 39, 291, 542, 569, 575, 657
Lsp1109I GCAGC 4 cut(s) 145, 322, 457, 505
LweI GCATC 2 cut(s) 457, 595
MaeII ACGT 1 cut(s) 199
MaeIII GTNAC 1 cut(s) 650
MalI GATC 1 cut(s) 326
MboI GATC 1 cut(s) 324
MboII GAAGA 6 cut(s) 58, 69, 77, 239, 247, 677
MhlI GDGCHC 1 cut(s) 549
MlsI TGGCCA 1 cut(s) 269
MluCI AATT 1 cut(s) 438
MluNI TGGCCA 1 cut(s) 269
MlyI GAGTC 1 cut(s) 623
MnlI CCTC 8 cut(s) 51, 61, 151, 216, 219, 300, 359, 682
Mox20I TGGCCA 1 cut(s) 269
MscI TGGCCA 1 cut(s) 269
MseI TTAA 4 cut(s) 29, 441, 567, 588
MslI CAYNNNNRTG 1 cut(s) 263
Msp20I TGGCCA 1 cut(s) 269
MspA1I CMGCKG 2 cut(s) 136, 398
MspI CCGG 1 cut(s) 644
MspR9I CCNGG 3 cut(s) 557, 644, 645
Mva1269I GAATGC 1 cut(s) 28
MvaI CCWGG 1 cut(s) 557
MvnI CGCG 1 cut(s) 187
MwoI GCNNNNNNNGC 1 cut(s) 634
NciI CCSGG 2 cut(s) 644, 645
NdeII GATC 1 cut(s) 324
NlaIII CATG 3 cut(s) 114, 331, 514
NmeAIII GCCGAG 2 cut(s) 13, 140
NmuCI GTSAC 1 cut(s) 650
NspI RCATGY 1 cut(s) 114
NspV TTCGAA 1 cut(s) 63
PaeI GCATGC 1 cut(s) 114
PcsI WCGNNNNNNNCGW 1 cut(s) 457
PctI GAATGC 1 cut(s) 28
PfeI GAWTC 3 cut(s) 60, 246, 424
PkrI GCNGC 7 cut(s) 135, 138, 184, 337, 397, 447, 520
PleI GAGTC 1 cut(s) 622
PpsI GAGTC 1 cut(s) 622
Psp124BI GAGCTC 1 cut(s) 549
Psp6I CCWGG 1 cut(s) 555
PspGI CCWGG 1 cut(s) 555
PspPI GGNCC 1 cut(s) 559
RsaI GTAC 5 cut(s) 129, 202, 313, 463, 529
RsaNI GTAC 5 cut(s) 128, 201, 312, 462, 528
RseI CAYNNNNRTG 1 cut(s) 263
SacI GAGCTC 1 cut(s) 549
SaqAI TTAA 4 cut(s) 29, 441, 567, 588
SatI GCNGC 7 cut(s) 134, 137, 183, 336, 396, 446, 519
Sau3AI GATC 1 cut(s) 324
Sau96I GGNCC 1 cut(s) 559
ScaI AGTACT 1 cut(s) 529
SchI GAGTC 1 cut(s) 623
ScrFI CCNGG 3 cut(s) 557, 644, 645
SduI GDGCHC 1 cut(s) 549
SfaNI GCATC 2 cut(s) 457, 595
SfuI TTCGAA 1 cut(s) 63
SinI GGWCC 1 cut(s) 559
SmaI CCCGGG 1 cut(s) 645
SmiMI CAYNNNNRTG 1 cut(s) 263
SmlI CTYRAG 1 cut(s) 100
SmoI CTYRAG 1 cut(s) 100
SphI GCATGC 1 cut(s) 114
Sse9I AATT 1 cut(s) 438
SsiI CCGC 5 cut(s) 125, 136, 139, 182, 396
SstI GAGCTC 1 cut(s) 549
StyD4I CCNGG 3 cut(s) 555, 642, 643
TaaI ACNGT 1 cut(s) 574
TaiI ACGT 1 cut(s) 202
TaqI TCGA 3 cut(s) 63, 478, 579
TasI AATT 1 cut(s) 438
TatI WGTACW 1 cut(s) 527
TauI GCSGC 3 cut(s) 139, 185, 398
TfiI GAWTC 3 cut(s) 60, 246, 424
Tru1I TTAA 4 cut(s) 29, 441, 567, 588
Tru9I TTAA 4 cut(s) 29, 441, 567, 588
TscAI CASTG 1 cut(s) 579
TseFI GTSAC 1 cut(s) 650
TseI GCWGC 4 cut(s) 133, 335, 445, 518
Tsp45I GTSAC 1 cut(s) 650
TspDTI ATGAA 1 cut(s) 247
TspGWI ACGGA 1 cut(s) 519
TspMI CCCGGG 1 cut(s) 643
TspRI CASTG 1 cut(s) 579
VpaK11BI GGWCC 1 cut(s) 559
XceI RCATGY 1 cut(s) 114
XmaI CCCGGG 1 cut(s) 643
ZrmI AGTACT 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.