FvH4_4g24830

metalloendopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011089
Physical Location & Seq
Forward (+)
26825622 .. 26828100
2479 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24830.t1

Sequence Viewer

Length: 702 bp
ATGGAAGTGGGTATGTCTTTAAACGCAGTGGTTCGTCTTCCACTATCGAGTTCAAGGACCCATGAAGACGGTTTGGTCAGGCACTCACTGGTGACCTCAAAAACGACCCAGAAAGCTTGCAAGCCAAGTCATGGCCAGACTCTGGTGGTTGAAGCAAAGGGTAAAAAGGGAATGCAAGATCGCCAATTTCAGAGGCGCCAACCTCCTCCCATGCCCAAGATTGAGGACGATGGCAACCCACGTTTCGTTGTGTTCATTCGAATGGCCAATGTTTACCTATGGTACCCACTTAGTGTGATCTCTGGTGGAACCACGGCAAAAATCATGCTTGCAGCTAAAGATAATTTTCTGGGGAAATATATTTACAAAGATACACTTGCTAGAAATCTTGCAGCTGTTATTTACCGAGATGAGAAGGAAATACAGAAGACGGCGTTCAAGCAGTATCGTGTATTGCGGTCAGCTACTGAGTTTAGATACGGCTACAAGATTGTGGAGAATGGTAATATGAGAGCAGCACTTGCAACCTCAGATGTAATTGAGCTTCCAACACCAGATCAGCTTAGAACCACTTTTGATAAAGTGAAAGATTTTTTTGGGGATGCAAAGGAATCTTTTGGCAAGCTGACGGCTATAGGTTCATCCGAGTCTGAGGAATCAGTAAAAGAAGTTGAAAAACCCAAGGAAAAGGTTAAAGGCTGA

Protein Analysis

234

Amino Acids

26.21

Weight (kDa)

9.6

Isoelectric Point (pI)

33.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HHL1-like PF20133 55 - 201 1.8e-11 Protein HHL1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011089)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67700 AT1G67700 AT1G67700 AT1G67700 AT1G67700
fragaria_vesca FvH4_4g24830
malus_domestica MD13G1108600.v1.1
prunus_persica Prupe.1G237600_v2.0.a1
pyrus_communis pycom13g09410
rosa_chinensis RchiOBHm_Chr4g0431911
rosa_laevigata RLG00000006876
rosa_multiflora Rmu_sc0004965.1_g000007
rosa_roxburghii Rroxscaffold_5G00373340
rosa_rugosa Rorug04G0253000 Rorug04G0253000
rosa_samantha Rh4AG310200 Rh4BG317300 Rh4CG332600 Rh4DG312200
rosa_wichuraiana Rw4G026990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 74
Acc65I GGTACC 1 cut(s) 282
AccB1I GGYRCC 2 cut(s) 195, 282
AccB7I CCANNNNNTGG 2 cut(s) 131, 142
AciI CCGC 1 cut(s) 457
AcoI YGGCCR 2 cut(s) 133, 264
AcyI GRCGYC 1 cut(s) 196
AdeI CACNNNGTG 1 cut(s) 293
AfaI GTAC 1 cut(s) 284
AfiI CCNNNNNNNGG 2 cut(s) 131, 142
AgsI TTSAA 4 cut(s) 54, 152, 439, 674
AluBI AGCT 7 cut(s) 116, 335, 395, 464, 544, 562, 625
AluI AGCT 7 cut(s) 116, 335, 395, 464, 544, 562, 625
AlwNI CAGNNNCTG 2 cut(s) 142, 467
AoxI GGCC 2 cut(s) 133, 264
ApeKI GCWGC 3 cut(s) 332, 392, 515
Asp718I GGTACC 1 cut(s) 282
AspLEI GCGC 1 cut(s) 198
AspS9I GGNCC 1 cut(s) 57
AsuHPI GGTGA 1 cut(s) 103
AsuII TTCGAA 1 cut(s) 259
AvaII GGWCC 1 cut(s) 57
BalI TGGCCA 2 cut(s) 135, 266
BanI GGYRCC 2 cut(s) 195, 282
BarI GAAGNNNNNNTAC 2 cut(s) 528, 560
BbsI GAAGAC 3 cut(s) 29, 72, 434
BbvI GCAGC 3 cut(s) 344, 404, 527
BccI CCATC 1 cut(s) 224
BceAI ACGGC 4 cut(s) 330, 447, 496, 645
BfaI CTAG 1 cut(s) 381
BfmI CTRYAG 1 cut(s) 633
BfoI RGCGCY 1 cut(s) 199
BisI GCNGC 3 cut(s) 333, 393, 516
BlsI GCNGC 3 cut(s) 334, 394, 517
Bme18I GGWCC 1 cut(s) 57
BmgT120I GGNCC 1 cut(s) 57
BmiI GGNNCC 4 cut(s) 59, 197, 284, 310
BmsI GCATC 1 cut(s) 592
BpiI GAAGAC 3 cut(s) 29, 72, 434
Bpu14I TTCGAA 1 cut(s) 259
BsaHI GRCGYC 1 cut(s) 196
BsaJI CCNNGG 2 cut(s) 312, 681
Bsc4I CCNNNNNNNGG 2 cut(s) 131, 142
Bse1I ACTGG 1 cut(s) 93
BseDI CCNNGG 2 cut(s) 312, 681
BseGI GGATG 2 cut(s) 607, 641
BseLI CCNNNNNNNGG 2 cut(s) 131, 142
BseMII CTCAG 3 cut(s) 459, 543, 642
BseNI ACTGG 1 cut(s) 93
BseRI GAGGAG 1 cut(s) 195
BseXI GCAGC 3 cut(s) 344, 404, 527
BshFI GGCC 2 cut(s) 135, 266
BshNI GGYRCC 2 cut(s) 195, 282
BslI CCNNNNNNNGG 2 cut(s) 131, 142
BsmI GAATGC 1 cut(s) 177
BsnI GGCC 2 cut(s) 135, 266
Bsp119I TTCGAA 1 cut(s) 259
Bsp143I GATC 3 cut(s) 178, 297, 556
BspACI CCGC 1 cut(s) 457
BspANI GGCC 2 cut(s) 135, 266
BspCNI CTCAG 3 cut(s) 460, 542, 643
BspLI GGNNCC 4 cut(s) 59, 197, 284, 310
BspT104I TTCGAA 1 cut(s) 259
BspT107I GGYRCC 2 cut(s) 195, 282
BsrI ACTGG 1 cut(s) 93
BssECI CCNNGG 2 cut(s) 312, 681
BssMI GATC 3 cut(s) 178, 297, 556
BssNI GRCGYC 1 cut(s) 196
BssT1I CCWWGG 1 cut(s) 681
Bst4CI ACNGT 1 cut(s) 71
BstACI GRCGYC 1 cut(s) 196
BstAPI GCANNNNNTGC 1 cut(s) 521
BstBI TTCGAA 1 cut(s) 259
BstC8I GCNNGC 4 cut(s) 118, 122, 330, 623
BstDEI CTNAG 5 cut(s) 290, 468, 529, 563, 651
BstDSI CCRYGG 1 cut(s) 312
BstEII GGTNACC 1 cut(s) 91
BstF5I GGATG 2 cut(s) 607, 641
BstH2I RGCGCY 1 cut(s) 199
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 3 cut(s) 181, 300, 559
BstMBI GATC 3 cut(s) 178, 297, 556
BstMWI GCNNNNNNNGC 1 cut(s) 521
BstPI GGTNACC 1 cut(s) 91
BstSFI CTRYAG 1 cut(s) 633
BstV1I GCAGC 3 cut(s) 344, 404, 527
BstV2I GAAGAC 3 cut(s) 29, 72, 434
BsuRI GGCC 2 cut(s) 135, 266
BtgI CCRYGG 1 cut(s) 312
BtsCI GGATG 2 cut(s) 607, 641
BtsI GCAGTG 1 cut(s) 33
BtsIMutI CAGTG 2 cut(s) 33, 86
Cac8I GCNNGC 4 cut(s) 118, 122, 330, 623
CaiI CAGNNNCTG 2 cut(s) 142, 467
CfoI GCGC 1 cut(s) 198
Cfr13I GGNCC 1 cut(s) 57
Csp6I GTAC 1 cut(s) 283
CviAII CATG 4 cut(s) 62, 131, 211, 325
CviQI GTAC 1 cut(s) 283
DdeI CTNAG 5 cut(s) 290, 468, 529, 563, 651
DinI GGCGCC 1 cut(s) 197
DpnI GATC 3 cut(s) 180, 299, 558
DpnII GATC 3 cut(s) 178, 297, 556
DraI TTTAAA 1 cut(s) 21
DraIII CACNNNGTG 1 cut(s) 293
DrdI GACNNNNNNGTC 1 cut(s) 74
DseDI GACNNNNNNGTC 1 cut(s) 74
EaeI YGGCCR 2 cut(s) 133, 264
Eco130I CCWWGG 1 cut(s) 681
Eco47I GGWCC 1 cut(s) 57
Eco91I GGTNACC 1 cut(s) 91
EcoO109I RGGNCCY 1 cut(s) 57
EcoO65I GGTNACC 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 681
EgeI GGCGCC 1 cut(s) 197
EheI GGCGCC 1 cut(s) 197
ErhI CCWWGG 1 cut(s) 681
FaeI CATG 4 cut(s) 65, 134, 214, 328
FaiI YATR 9 cut(s) 14, 63, 132, 212, 280, 326, 360, 509, 635
FalI AAGNNNNNCTT 2 cut(s) 360, 392
FatI CATG 4 cut(s) 61, 130, 210, 324
Fnu4HI GCNGC 3 cut(s) 333, 393, 516
FokI GGATG 2 cut(s) 614, 628
Fsp4HI GCNGC 3 cut(s) 333, 393, 516
FspBI CTAG 1 cut(s) 381
GlaI GCGC 1 cut(s) 197
GluI GCNGC 3 cut(s) 333, 393, 516
HaeII RGCGCY 1 cut(s) 199
HaeIII GGCC 2 cut(s) 135, 266
HhaI GCGC 1 cut(s) 198
Hin1I GRCGYC 1 cut(s) 196
Hin1II CATG 4 cut(s) 65, 134, 214, 328
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HindIII AAGCTT 1 cut(s) 114
HinfI GANTC 4 cut(s) 139, 611, 647, 656
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 4 cut(s) 192, 532, 646, 652
Hpy8I GTNNAC 1 cut(s) 274
HpyAV CCTTC 1 cut(s) 409
HpyCH4III ACNGT 1 cut(s) 71
HpyCH4IV ACGT 1 cut(s) 241
HpyCH4V TGCA 6 cut(s) 120, 175, 332, 392, 524, 605
HpyF10VI GCNNNNNNNGC 1 cut(s) 521
HpyF3I CTNAG 5 cut(s) 290, 468, 529, 563, 651
HpySE526I ACGT 1 cut(s) 241
Hsp92I GRCGYC 1 cut(s) 196
Hsp92II CATG 4 cut(s) 65, 134, 214, 328
HspAI GCGC 1 cut(s) 196
KasI GGCGCC 1 cut(s) 195
KpnI GGTACC 1 cut(s) 286
Kzo9I GATC 3 cut(s) 178, 297, 556
LpnPI CCDG 8 cut(s) 64, 74, 122, 128, 149, 288, 335, 567
Lsp1109I GCAGC 3 cut(s) 344, 404, 527
LweI GCATC 1 cut(s) 592
MaeI CTAG 1 cut(s) 381
MaeII ACGT 1 cut(s) 241
MaeIII GTNAC 1 cut(s) 91
MalI GATC 3 cut(s) 180, 299, 558
MboI GATC 3 cut(s) 178, 297, 556
MboII GAAGA 3 cut(s) 29, 77, 439
MlsI TGGCCA 2 cut(s) 135, 266
MluCI AATT 3 cut(s) 185, 343, 537
MluNI TGGCCA 2 cut(s) 135, 266
Mly113I GGCGCC 1 cut(s) 196
MlyI GAGTC 2 cut(s) 133, 656
MmeI TCCRAC 1 cut(s) 572
MnlI CCTC 7 cut(s) 106, 186, 213, 216, 217, 538, 646
Mox20I TGGCCA 2 cut(s) 135, 266
MscI TGGCCA 2 cut(s) 135, 266
MseI TTAA 2 cut(s) 20, 693
MslI CAYNNNNRTG 1 cut(s) 260
Msp20I TGGCCA 2 cut(s) 135, 266
MspA1I CMGCKG 1 cut(s) 395
Mva1269I GAATGC 1 cut(s) 177
MwoI GCNNNNNNNGC 1 cut(s) 521
NarI GGCGCC 1 cut(s) 196
NdeII GATC 3 cut(s) 178, 297, 556
NlaIII CATG 4 cut(s) 65, 134, 214, 328
NlaIV GGNNCC 4 cut(s) 59, 197, 284, 310
NmuCI GTSAC 1 cut(s) 91
NspV TTCGAA 1 cut(s) 259
PctI GAATGC 1 cut(s) 177
PfeI GAWTC 2 cut(s) 611, 656
PflMI CCANNNNNTGG 2 cut(s) 131, 142
PkrI GCNGC 3 cut(s) 334, 394, 517
PleI GAGTC 2 cut(s) 133, 655
PluTI GGCGCC 1 cut(s) 199
PpsI GAGTC 2 cut(s) 133, 655
PpuMI RGGWCCY 1 cut(s) 57
Psp5II RGGWCCY 1 cut(s) 57
PspEI GGTNACC 1 cut(s) 91
PspN4I GGNNCC 4 cut(s) 59, 197, 284, 310
PspPI GGNCC 1 cut(s) 57
PspPPI RGGWCCY 1 cut(s) 57
PstNI CAGNNNCTG 2 cut(s) 142, 467
PvuII CAGCTG 1 cut(s) 395
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
RseI CAYNNNNRTG 1 cut(s) 260
SaqAI TTAA 2 cut(s) 20, 693
SatI GCNGC 3 cut(s) 333, 393, 516
Sau3AI GATC 3 cut(s) 178, 297, 556
Sau96I GGNCC 1 cut(s) 57
SchI GAGTC 2 cut(s) 133, 656
SfaNI GCATC 1 cut(s) 592
SfcI CTRYAG 1 cut(s) 633
SfoI GGCGCC 1 cut(s) 197
SfuI TTCGAA 1 cut(s) 259
SinI GGWCC 1 cut(s) 57
SmiMI CAYNNNNRTG 1 cut(s) 260
Sse9I AATT 3 cut(s) 185, 343, 537
SsiI CCGC 1 cut(s) 457
SspDI GGCGCC 1 cut(s) 195
SspMI CTAG 1 cut(s) 381
StyI CCWWGG 1 cut(s) 681
TaaI ACNGT 1 cut(s) 71
TaiI ACGT 1 cut(s) 244
TaqI TCGA 2 cut(s) 47, 259
TasI AATT 3 cut(s) 185, 343, 537
TfiI GAWTC 2 cut(s) 611, 656
Tru1I TTAA 2 cut(s) 20, 693
Tru9I TTAA 2 cut(s) 20, 693
TscAI CASTG 2 cut(s) 33, 93
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 3 cut(s) 332, 392, 515
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 3 cut(s) 78, 244, 630
TspRI CASTG 2 cut(s) 33, 93
Van91I CCANNNNNTGG 2 cut(s) 131, 142
VpaK11BI GGWCC 1 cut(s) 57
XspI CTAG 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.