Rh4BG317300

metalloendopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0011089
Physical Location & Seq
Forward (+)
48465677 .. 48467608
1932 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG317300.1

Sequence Viewer

Length: 708 bp
ATGGAAGTGGGTATGTCTTTGTCTTTAAACGCAGTGGTTCGGCTTCCACTATCGAATTCAAGAACCCATGAAGACGGTTTGGTCAGGCACTCACTGGTGTCCTCAAAAACGACCCAGAAAGCTTGTAAGCAAAGCCATGGCCAAGCACTGGTGATTGAAGCAAAGGGCAAAAAGGGAATGCAAGATCGTCAGTTCCAGAGGCGTCAACCTCCTCCCATGCCCAAGATTGAAGACGATGGCAACCCACGTTTCGTTGTGTTTATTCGAATGGCCAATGTTTACCTATGGTATCCACTTAGTGTGATCTCTGGTGGAACCACGGCAAAAATCATGCTTGCAGCGAAAGATAATTTTCTGGGGAAATATATATACAAAGATACACTTGCTAGAAATCTTGCAGCAGTTATTTACCGAGATGAGAAGGAAATTCAGAAGACGGCGTTCAAGCAGTATCGTGTATTGCGGTCAGCGACTGAGTTTAGATATGGCTACAAGATTGTGGAAAATGGTAATATGAGAGCAGCACTTTCAACGTCAGATGTAATTGAGCTTCCAACACCAGACCAGCTTAGAACCACTCTTGATAAAGTGAAAGATTTTTTTGGGGAAGCAAAGGAATCTTTTGGCAAGCTGACGGCTATAAGTTCATCCGAGTCTGAGGAATCAGAACAAAAAATTGAAAAACCCAAGGAAAAGGTTAAACGCTGA

Protein Analysis

235

Amino Acids

26.61

Weight (kDa)

9.66

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HHL1-like PF20133 57 - 202 5.6e-11 Protein HHL1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011089)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67700 AT1G67700 AT1G67700 AT1G67700 AT1G67700
fragaria_vesca FvH4_4g24830
malus_domestica MD13G1108600.v1.1
prunus_persica Prupe.1G237600_v2.0.a1
pyrus_communis pycom13g09410
rosa_chinensis RchiOBHm_Chr4g0431911
rosa_laevigata RLG00000006876
rosa_multiflora Rmu_sc0004965.1_g000007
rosa_roxburghii Rroxscaffold_5G00373340
rosa_rugosa Rorug04G0253000 Rorug04G0253000
rosa_samantha Rh4AG310200 Rh4BG317300 Rh4CG332600 Rh4DG312200
rosa_wichuraiana Rw4G026990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 80
AccB7I CCANNNNNTGG 1 cut(s) 148
AciI CCGC 1 cut(s) 463
AcoI YGGCCR 2 cut(s) 139, 270
AcsI RAATTY 2 cut(s) 55, 426
AcyI GRCGYC 1 cut(s) 202
AdeI CACNNNGTG 1 cut(s) 299
AfiI CCNNNNNNNGG 1 cut(s) 148
AgsI TTSAA 6 cut(s) 60, 158, 230, 445, 531, 680
AluBI AGCT 4 cut(s) 122, 550, 568, 631
AluI AGCT 4 cut(s) 122, 550, 568, 631
AlwNI CAGNNNCTG 1 cut(s) 473
AoxI GGCC 2 cut(s) 139, 270
ApeKI GCWGC 3 cut(s) 338, 398, 521
ApoI RAATTY 2 cut(s) 55, 426
AsuHPI GGTGA 1 cut(s) 163
AsuII TTCGAA 1 cut(s) 265
BalI TGGCCA 2 cut(s) 141, 272
BarI GAAGNNNNNNTAC 2 cut(s) 534, 566
BbsI GAAGAC 3 cut(s) 78, 237, 440
BbvI GCAGC 3 cut(s) 350, 410, 533
BccI CCATC 1 cut(s) 230
BceAI ACGGC 3 cut(s) 336, 453, 651
BciVI GTATCC 1 cut(s) 300
BfaI CTAG 1 cut(s) 387
BfuI GTATCC 1 cut(s) 300
BisI GCNGC 3 cut(s) 339, 399, 522
BlsI GCNGC 3 cut(s) 340, 400, 523
BmiI GGNNCC 1 cut(s) 316
BpiI GAAGAC 3 cut(s) 78, 237, 440
Bpu14I TTCGAA 1 cut(s) 265
BsaHI GRCGYC 1 cut(s) 202
BsaJI CCNNGG 3 cut(s) 136, 318, 687
Bsc4I CCNNNNNNNGG 1 cut(s) 148
Bse1I ACTGG 2 cut(s) 99, 153
BseDI CCNNGG 3 cut(s) 136, 318, 687
BseGI GGATG 1 cut(s) 647
BseLI CCNNNNNNNGG 1 cut(s) 148
BseMII CTCAG 2 cut(s) 465, 648
BseNI ACTGG 2 cut(s) 99, 153
BseRI GAGGAG 1 cut(s) 201
BseXI GCAGC 3 cut(s) 350, 410, 533
BshFI GGCC 2 cut(s) 141, 272
BslI CCNNNNNNNGG 1 cut(s) 148
BsmI GAATGC 1 cut(s) 183
BsnI GGCC 2 cut(s) 141, 272
Bsp119I TTCGAA 1 cut(s) 265
Bsp143I GATC 2 cut(s) 184, 303
Bsp19I CCATGG 1 cut(s) 136
BspACI CCGC 1 cut(s) 463
BspANI GGCC 2 cut(s) 141, 272
BspCNI CTCAG 2 cut(s) 466, 649
BspLI GGNNCC 1 cut(s) 316
BspT104I TTCGAA 1 cut(s) 265
BsrI ACTGG 2 cut(s) 99, 153
BssECI CCNNGG 3 cut(s) 136, 318, 687
BssMI GATC 2 cut(s) 184, 303
BssNI GRCGYC 1 cut(s) 202
BssT1I CCWWGG 2 cut(s) 136, 687
Bst4CI ACNGT 1 cut(s) 77
BstACI GRCGYC 1 cut(s) 202
BstBI TTCGAA 1 cut(s) 265
BstC8I GCNNGC 2 cut(s) 336, 629
BstDEI CTNAG 4 cut(s) 296, 474, 569, 657
BstDSI CCRYGG 2 cut(s) 136, 318
BstF5I GGATG 1 cut(s) 647
BstKTI GATC 2 cut(s) 187, 306
BstMBI GATC 2 cut(s) 184, 303
BstV1I GCAGC 3 cut(s) 350, 410, 533
BstV2I GAAGAC 3 cut(s) 78, 237, 440
BsuI GTATCC 1 cut(s) 300
BsuRI GGCC 2 cut(s) 141, 272
BtgI CCRYGG 2 cut(s) 136, 318
BtsCI GGATG 1 cut(s) 647
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 3 cut(s) 39, 92, 146
Cac8I GCNNGC 2 cut(s) 336, 629
CaiI CAGNNNCTG 1 cut(s) 473
CseI GACGC 1 cut(s) 191
CviAII CATG 4 cut(s) 68, 137, 217, 331
DdeI CTNAG 4 cut(s) 296, 474, 569, 657
DpnI GATC 2 cut(s) 186, 305
DpnII GATC 2 cut(s) 184, 303
DraI TTTAAA 1 cut(s) 27
DraIII CACNNNGTG 1 cut(s) 299
DrdI GACNNNNNNGTC 1 cut(s) 80
DseDI GACNNNNNNGTC 1 cut(s) 80
EaeI YGGCCR 2 cut(s) 139, 270
Eco130I CCWWGG 2 cut(s) 136, 687
EcoRI GAATTC 1 cut(s) 55
EcoT14I CCWWGG 2 cut(s) 136, 687
ErhI CCWWGG 2 cut(s) 136, 687
FaeI CATG 4 cut(s) 71, 140, 220, 334
FalI AAGNNNNNCTT 2 cut(s) 366, 398
FatI CATG 4 cut(s) 67, 136, 216, 330
Fnu4HI GCNGC 3 cut(s) 339, 399, 522
FokI GGATG 1 cut(s) 634
Fsp4HI GCNGC 3 cut(s) 339, 399, 522
FspBI CTAG 1 cut(s) 387
GluI GCNGC 3 cut(s) 339, 399, 522
HaeIII GGCC 2 cut(s) 141, 272
HgaI GACGC 1 cut(s) 191
Hin1I GRCGYC 1 cut(s) 202
Hin1II CATG 4 cut(s) 71, 140, 220, 334
HincII GTYRAC 1 cut(s) 206
HindII GTYRAC 1 cut(s) 206
HindIII AAGCTT 1 cut(s) 120
HinfI GANTC 3 cut(s) 617, 653, 662
HphI GGTGA 1 cut(s) 163
Hpy166II GTNNAC 2 cut(s) 206, 280
Hpy188I TCNGA 5 cut(s) 432, 538, 652, 658, 667
Hpy188III TCNNGA 3 cut(s) 60, 196, 581
Hpy8I GTNNAC 2 cut(s) 206, 280
HpyAV CCTTC 1 cut(s) 415
HpyCH4III ACNGT 1 cut(s) 77
HpyCH4IV ACGT 2 cut(s) 247, 533
HpyCH4V TGCA 3 cut(s) 181, 338, 398
HpyF3I CTNAG 4 cut(s) 296, 474, 569, 657
HpySE526I ACGT 2 cut(s) 247, 533
Hsp92I GRCGYC 1 cut(s) 202
Hsp92II CATG 4 cut(s) 71, 140, 220, 334
Kzo9I GATC 2 cut(s) 184, 303
LpnPI CCDG 9 cut(s) 70, 80, 128, 134, 209, 294, 341, 573, 578
Lsp1109I GCAGC 3 cut(s) 350, 410, 533
MaeI CTAG 1 cut(s) 387
MaeII ACGT 2 cut(s) 247, 533
MalI GATC 2 cut(s) 186, 305
MboI GATC 2 cut(s) 184, 303
MboII GAAGA 3 cut(s) 83, 242, 445
MlsI TGGCCA 2 cut(s) 141, 272
MluCI AATT 5 cut(s) 55, 349, 426, 543, 675
MluNI TGGCCA 2 cut(s) 141, 272
MlyI GAGTC 1 cut(s) 662
MmeI TCCRAC 1 cut(s) 578
MnlI CCTC 5 cut(s) 112, 192, 219, 222, 652
Mox20I TGGCCA 2 cut(s) 141, 272
MscI TGGCCA 2 cut(s) 141, 272
MseI TTAA 2 cut(s) 26, 699
Msp20I TGGCCA 2 cut(s) 141, 272
Mva1269I GAATGC 1 cut(s) 183
NcoI CCATGG 1 cut(s) 136
NdeII GATC 2 cut(s) 184, 303
NlaIII CATG 4 cut(s) 71, 140, 220, 334
NlaIV GGNNCC 1 cut(s) 316
NspV TTCGAA 1 cut(s) 265
PctI GAATGC 1 cut(s) 183
PfeI GAWTC 2 cut(s) 617, 662
PflMI CCANNNNNTGG 1 cut(s) 148
PkrI GCNGC 3 cut(s) 340, 400, 523
PleI GAGTC 1 cut(s) 661
PpsI GAGTC 1 cut(s) 661
PspN4I GGNNCC 1 cut(s) 316
PstNI CAGNNNCTG 1 cut(s) 473
SaqAI TTAA 2 cut(s) 26, 699
SatI GCNGC 3 cut(s) 339, 399, 522
Sau3AI GATC 2 cut(s) 184, 303
SchI GAGTC 1 cut(s) 662
SetI ASST 9 cut(s) 124, 211, 250, 285, 536, 552, 570, 633, 699
SfuI TTCGAA 1 cut(s) 265
Sse9I AATT 5 cut(s) 55, 349, 426, 543, 675
SsiI CCGC 1 cut(s) 463
SspMI CTAG 1 cut(s) 387
StyI CCWWGG 2 cut(s) 136, 687
TaaI ACNGT 1 cut(s) 77
TaiI ACGT 2 cut(s) 250, 536
TaqI TCGA 2 cut(s) 53, 265
TasI AATT 5 cut(s) 55, 349, 426, 543, 675
TfiI GAWTC 2 cut(s) 617, 662
Tru1I TTAA 2 cut(s) 26, 699
Tru9I TTAA 2 cut(s) 26, 699
TscAI CASTG 3 cut(s) 39, 99, 153
TseI GCWGC 3 cut(s) 338, 398, 521
TspDTI ATGAA 2 cut(s) 84, 636
TspRI CASTG 3 cut(s) 39, 99, 153
Van91I CCANNNNNTGG 1 cut(s) 148
XapI RAATTY 2 cut(s) 55, 426
XspI CTAG 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.