Rroxscaffold_5G00373340

metalloendopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0011089
Physical Location & Seq
Forward (+)
54606848 .. 54609101
2254 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00373340.1

Sequence Viewer

Length: 702 bp
ATGGAAGTGGGTATGTCTTTAAACGCAGTGGTTCGGCTTCCACTATCGAATTCAAGAACCCATGAAGACGGTTTGGTCAGGCACTCACTGGTGTCCTCAAAAACGACCCAGAAAGCTTGTAAGCAAAGCCATGGCCAAGCACTGGTGATTGAAGCAAAGGGAAAAAAGGGAATGCAAGATCGTCAGTTCCAGAGGCGTCAACCTCCTCCCATGCCCAAGATTGAAGACGATGGCAACCCACGTTTTGTTGTGTTTATTCGAATGGCCAATGTTTACCTATGGTACCCACTTAGTGTGATCTCTGGTGGAACCACGGCAAAAATCATGCTTGCAGCGAAAGATAATTTTCTGGGGAAATATATATACAAAGATACACTTGCTAGAAATCTTGCAGCAGTTATTTACCGAGATGAGAAGGAAATACAGAAGACGGCATTCAAGCAGTATCGTGTATTGCGGTCAGCGACTGAGTTTAGATATGGCTACAAGATTGTGGAAAATGGTAATATGAGAGCAGCACTTTCAACGTCAGATGTAATTGAGCTTCCTACACCAGACCAGCTTAGAACCACTCTTGATAAAGTGAAAGATTTTTTTGGGGAAGCAAAGGAATCTTTTGGCAAGCTGACGGCTATAAGTTCATCCGAGTCTGAGGAATCAGAACAAAAAATTGAAAAACCCAAGGAAAAGGTTAAACGCTGA

Protein Analysis

233

Amino Acids

26.41

Weight (kDa)

9.66

Isoelectric Point (pI)

42.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HHL1-like PF20133 55 - 200 5.4e-11 Protein HHL1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011089)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67700 AT1G67700 AT1G67700 AT1G67700 AT1G67700
fragaria_vesca FvH4_4g24830
malus_domestica MD13G1108600.v1.1
prunus_persica Prupe.1G237600_v2.0.a1
pyrus_communis pycom13g09410
rosa_chinensis RchiOBHm_Chr4g0431911
rosa_laevigata RLG00000006876
rosa_multiflora Rmu_sc0004965.1_g000007
rosa_roxburghii Rroxscaffold_5G00373340
rosa_rugosa Rorug04G0253000 Rorug04G0253000
rosa_samantha Rh4AG310200 Rh4BG317300 Rh4CG332600 Rh4DG312200
rosa_wichuraiana Rw4G026990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 74
Acc65I GGTACC 1 cut(s) 282
AccB1I GGYRCC 1 cut(s) 282
AccB7I CCANNNNNTGG 1 cut(s) 142
AciI CCGC 1 cut(s) 457
AcoI YGGCCR 2 cut(s) 133, 264
AcsI RAATTY 1 cut(s) 49
AcyI GRCGYC 1 cut(s) 196
AdeI CACNNNGTG 1 cut(s) 293
AfaI GTAC 1 cut(s) 284
AfiI CCNNNNNNNGG 1 cut(s) 142
AgsI TTSAA 6 cut(s) 54, 152, 224, 439, 525, 674
AluBI AGCT 4 cut(s) 116, 544, 562, 625
AluI AGCT 4 cut(s) 116, 544, 562, 625
AlwNI CAGNNNCTG 1 cut(s) 467
AoxI GGCC 2 cut(s) 133, 264
ApeKI GCWGC 3 cut(s) 332, 392, 515
ApoI RAATTY 1 cut(s) 49
Asp718I GGTACC 1 cut(s) 282
AsuHPI GGTGA 1 cut(s) 157
AsuII TTCGAA 1 cut(s) 259
BalI TGGCCA 2 cut(s) 135, 266
BanI GGYRCC 1 cut(s) 282
BarI GAAGNNNNNNTAC 2 cut(s) 528, 560
BbsI GAAGAC 3 cut(s) 72, 231, 434
BbvI GCAGC 3 cut(s) 344, 404, 527
BccI CCATC 1 cut(s) 224
BceAI ACGGC 3 cut(s) 330, 447, 645
BfaI CTAG 1 cut(s) 381
BisI GCNGC 3 cut(s) 333, 393, 516
BlsI GCNGC 3 cut(s) 334, 394, 517
BmiI GGNNCC 2 cut(s) 284, 310
BpiI GAAGAC 3 cut(s) 72, 231, 434
Bpu14I TTCGAA 1 cut(s) 259
BsaHI GRCGYC 1 cut(s) 196
BsaJI CCNNGG 3 cut(s) 130, 312, 681
Bsc4I CCNNNNNNNGG 1 cut(s) 142
Bse1I ACTGG 2 cut(s) 93, 147
BseDI CCNNGG 3 cut(s) 130, 312, 681
BseGI GGATG 1 cut(s) 641
BseLI CCNNNNNNNGG 1 cut(s) 142
BseMII CTCAG 2 cut(s) 459, 642
BseNI ACTGG 2 cut(s) 93, 147
BseRI GAGGAG 1 cut(s) 195
BseXI GCAGC 3 cut(s) 344, 404, 527
BshFI GGCC 2 cut(s) 135, 266
BshNI GGYRCC 1 cut(s) 282
BslI CCNNNNNNNGG 1 cut(s) 142
BsmI GAATGC 2 cut(s) 177, 434
BsnI GGCC 2 cut(s) 135, 266
Bsp119I TTCGAA 1 cut(s) 259
Bsp143I GATC 2 cut(s) 178, 297
Bsp19I CCATGG 1 cut(s) 130
BspACI CCGC 1 cut(s) 457
BspANI GGCC 2 cut(s) 135, 266
BspCNI CTCAG 2 cut(s) 460, 643
BspLI GGNNCC 2 cut(s) 284, 310
BspT104I TTCGAA 1 cut(s) 259
BspT107I GGYRCC 1 cut(s) 282
BsrI ACTGG 2 cut(s) 93, 147
BssECI CCNNGG 3 cut(s) 130, 312, 681
BssMI GATC 2 cut(s) 178, 297
BssNI GRCGYC 1 cut(s) 196
BssT1I CCWWGG 2 cut(s) 130, 681
Bst4CI ACNGT 1 cut(s) 71
BstACI GRCGYC 1 cut(s) 196
BstBI TTCGAA 1 cut(s) 259
BstC8I GCNNGC 2 cut(s) 330, 623
BstDEI CTNAG 4 cut(s) 290, 468, 563, 651
BstDSI CCRYGG 2 cut(s) 130, 312
BstF5I GGATG 1 cut(s) 641
BstKTI GATC 2 cut(s) 181, 300
BstMBI GATC 2 cut(s) 178, 297
BstV1I GCAGC 3 cut(s) 344, 404, 527
BstV2I GAAGAC 3 cut(s) 72, 231, 434
BsuRI GGCC 2 cut(s) 135, 266
BtgI CCRYGG 2 cut(s) 130, 312
BtsCI GGATG 1 cut(s) 641
BtsI GCAGTG 1 cut(s) 33
BtsIMutI CAGTG 3 cut(s) 33, 86, 140
Cac8I GCNNGC 2 cut(s) 330, 623
CaiI CAGNNNCTG 1 cut(s) 467
CseI GACGC 1 cut(s) 185
Csp6I GTAC 1 cut(s) 283
CviAII CATG 4 cut(s) 62, 131, 211, 325
CviQI GTAC 1 cut(s) 283
DdeI CTNAG 4 cut(s) 290, 468, 563, 651
DpnI GATC 2 cut(s) 180, 299
DpnII GATC 2 cut(s) 178, 297
DraI TTTAAA 1 cut(s) 21
DraIII CACNNNGTG 1 cut(s) 293
DrdI GACNNNNNNGTC 1 cut(s) 74
DseDI GACNNNNNNGTC 1 cut(s) 74
EaeI YGGCCR 2 cut(s) 133, 264
Eco130I CCWWGG 2 cut(s) 130, 681
EcoRI GAATTC 1 cut(s) 49
EcoT14I CCWWGG 2 cut(s) 130, 681
ErhI CCWWGG 2 cut(s) 130, 681
FaeI CATG 4 cut(s) 65, 134, 214, 328
FalI AAGNNNNNCTT 2 cut(s) 360, 392
FatI CATG 4 cut(s) 61, 130, 210, 324
Fnu4HI GCNGC 3 cut(s) 333, 393, 516
FokI GGATG 1 cut(s) 628
Fsp4HI GCNGC 3 cut(s) 333, 393, 516
FspBI CTAG 1 cut(s) 381
GluI GCNGC 3 cut(s) 333, 393, 516
HaeIII GGCC 2 cut(s) 135, 266
HgaI GACGC 1 cut(s) 185
Hin1I GRCGYC 1 cut(s) 196
Hin1II CATG 4 cut(s) 65, 134, 214, 328
HincII GTYRAC 1 cut(s) 200
HindII GTYRAC 1 cut(s) 200
HindIII AAGCTT 1 cut(s) 114
HinfI GANTC 3 cut(s) 611, 647, 656
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 2 cut(s) 200, 274
Hpy188I TCNGA 4 cut(s) 532, 646, 652, 661
Hpy188III TCNNGA 3 cut(s) 54, 190, 575
Hpy8I GTNNAC 2 cut(s) 200, 274
HpyAV CCTTC 1 cut(s) 409
HpyCH4III ACNGT 1 cut(s) 71
HpyCH4IV ACGT 2 cut(s) 241, 527
HpyCH4V TGCA 3 cut(s) 175, 332, 392
HpyF3I CTNAG 4 cut(s) 290, 468, 563, 651
HpySE526I ACGT 2 cut(s) 241, 527
Hsp92I GRCGYC 1 cut(s) 196
Hsp92II CATG 4 cut(s) 65, 134, 214, 328
KpnI GGTACC 1 cut(s) 286
Kzo9I GATC 2 cut(s) 178, 297
LpnPI CCDG 9 cut(s) 64, 74, 122, 128, 203, 288, 335, 567, 572
Lsp1109I GCAGC 3 cut(s) 344, 404, 527
MaeI CTAG 1 cut(s) 381
MaeII ACGT 2 cut(s) 241, 527
MalI GATC 2 cut(s) 180, 299
MboI GATC 2 cut(s) 178, 297
MboII GAAGA 3 cut(s) 77, 236, 439
MlsI TGGCCA 2 cut(s) 135, 266
MluCI AATT 4 cut(s) 49, 343, 537, 669
MluNI TGGCCA 2 cut(s) 135, 266
MlyI GAGTC 1 cut(s) 656
MnlI CCTC 5 cut(s) 106, 186, 213, 216, 646
Mox20I TGGCCA 2 cut(s) 135, 266
MscI TGGCCA 2 cut(s) 135, 266
MseI TTAA 2 cut(s) 20, 693
Msp20I TGGCCA 2 cut(s) 135, 266
Mva1269I GAATGC 2 cut(s) 177, 434
NcoI CCATGG 1 cut(s) 130
NdeII GATC 2 cut(s) 178, 297
NlaIII CATG 4 cut(s) 65, 134, 214, 328
NlaIV GGNNCC 2 cut(s) 284, 310
NspV TTCGAA 1 cut(s) 259
PctI GAATGC 2 cut(s) 177, 434
PfeI GAWTC 2 cut(s) 611, 656
PflMI CCANNNNNTGG 1 cut(s) 142
PkrI GCNGC 3 cut(s) 334, 394, 517
PleI GAGTC 1 cut(s) 655
PpsI GAGTC 1 cut(s) 655
PspN4I GGNNCC 2 cut(s) 284, 310
PstNI CAGNNNCTG 1 cut(s) 467
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
SaqAI TTAA 2 cut(s) 20, 693
SatI GCNGC 3 cut(s) 333, 393, 516
Sau3AI GATC 2 cut(s) 178, 297
SchI GAGTC 1 cut(s) 656
SetI ASST 9 cut(s) 118, 205, 244, 279, 530, 546, 564, 627, 693
SfuI TTCGAA 1 cut(s) 259
Sse9I AATT 4 cut(s) 49, 343, 537, 669
SsiI CCGC 1 cut(s) 457
SspMI CTAG 1 cut(s) 381
StyI CCWWGG 2 cut(s) 130, 681
TaaI ACNGT 1 cut(s) 71
TaiI ACGT 2 cut(s) 244, 530
TaqI TCGA 2 cut(s) 47, 259
TasI AATT 4 cut(s) 49, 343, 537, 669
TfiI GAWTC 2 cut(s) 611, 656
Tru1I TTAA 2 cut(s) 20, 693
Tru9I TTAA 2 cut(s) 20, 693
TscAI CASTG 3 cut(s) 33, 93, 147
TseI GCWGC 3 cut(s) 332, 392, 515
TspDTI ATGAA 2 cut(s) 78, 630
TspRI CASTG 3 cut(s) 33, 93, 147
Van91I CCANNNNNTGG 1 cut(s) 142
XapI RAATTY 1 cut(s) 49
XspI CTAG 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.