MD16G1108600.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015604
Physical Location & Seq
Forward (+)
7581626 .. 7584321
2696 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1108600.v1.1.491

Sequence Viewer

Length: 711 bp
ATGTCTCAGGCTATAGCAAATTTCAGCCTCTTTTCTCCCAATGTCATCTGCAAAAAACAAAAATGTGCGATACCAGTTTTTTCCAAACCAATTCATGGCGGTGGACGTTTTAGCTACGGTAAGAACAGAATCAAGGCCATGGCAGGCGATGCACGAGACAACCTCGACCACTTGCAGAGGACCGCCAAGCTCCAACAACAACAACAACAACAAGCCCAGCCTAGAAAGAGAGCTGCCCCAGCGCCACCCATCGGGTTGTGGGATCGGTTTCCAACGGCGAGGACGGTGCAGCAGATGATGGAAACAATGGAGAGGATGATGGAGGACCCATTGGCCTACTCTGGCGGGTACACGCCGCCATTGCCAAGAGAGGCAGAAGTGTACGGCAGGGGACGGACGCCATGGGAGATCAAAGAAGGGGAGAGTGATTACAAGATGAGATTCGACATGCCGGGGATGACGAAAGAGGACGTGAAGGTGTGGGTGGAGGATAAAATGCTGGTTGTGAAGGCTGAGAAGGCGGCCAAGAAGACGGAGAATGGGGTGGTGGTGGAAGAAAATGATAGGATTAAAAAGCACACCACAAAGATTGCGTTGCCGGAGAATATTCAGTTTGAGGAGATCAAGGCTGAGGTTAAAGATGGGGTCTTGTATATTACTATTCCGAAGGCTTCCGTTACTTCCAAGATTTTGGACATCAATGTTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

237

Amino Acids

26.71

Weight (kDa)

9.36

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 136 - 228 1e-21 Hsp20/alpha crystallin family
ArsA_HSP20 PF17886 142 - 220 1.2e-07 HSP20-like domain found in ArsA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015604)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24850
malus_domestica MD13G1108500.v1.1 MD16G1108600.v1.1
prunus_persica Prupe.1G237800_v2.0.a1
pyrus_communis pycom13g09400 pycom16g09270
rosa_chinensis RchiOBHm_Chr4g0431921
rosa_laevigata RLG00000006875
rosa_multiflora Rmu_sc0004965.1_g000008
rosa_roxburghii Rroxscaffold_5G00373350
rosa_rugosa Rorug04G0253000
rosa_samantha Rh4AG310300 Rh4BG317400 Rh4CG332700 Rh4DG312300
rosa_wichuraiana Rw4G027000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 95
AciI CCGC 5 cut(s) 99, 183, 345, 356, 521
AclWI GGATC 1 cut(s) 270
AcoI YGGCCR 1 cut(s) 522
AcsI RAATTY 1 cut(s) 19
AcyI GRCGYC 1 cut(s) 398
AfaI GTAC 2 cut(s) 350, 383
AfiI CCNNNNNNNGG 2 cut(s) 95, 251
AjiI CACGTC 1 cut(s) 472
AluBI AGCT 3 cut(s) 114, 190, 233
AluI AGCT 3 cut(s) 114, 190, 233
Alw26I GTCTC 2 cut(s) 9, 150
AlwI GGATC 1 cut(s) 270
AoxI GGCC 3 cut(s) 135, 333, 522
ApeKI GCWGC 2 cut(s) 233, 289
ApoI RAATTY 1 cut(s) 19
AspLEI GCGC 1 cut(s) 244
AspS9I GGNCC 2 cut(s) 180, 325
AsuC2I CCSGG 1 cut(s) 453
AvaII GGWCC 2 cut(s) 180, 325
BauI CACGAG 1 cut(s) 153
BbsI GAAGAC 1 cut(s) 536
BbvCI CCTCAGC 1 cut(s) 630
BbvI GCAGC 2 cut(s) 220, 301
BccI CCATC 4 cut(s) 257, 292, 313, 635
BceAI ACGGC 2 cut(s) 291, 400
BcgI CGANNNNNNTGC 2 cut(s) 268, 302
BcnI CCSGG 1 cut(s) 453
BcoDI GTCTC 2 cut(s) 9, 150
BfaI CTAG 1 cut(s) 222
BfmI CTRYAG 1 cut(s) 12
BfoI RGCGCY 1 cut(s) 245
BisI GCNGC 4 cut(s) 234, 290, 356, 522
BlsI GCNGC 4 cut(s) 235, 291, 357, 523
Bme1390I CCNGG 1 cut(s) 453
Bme18I GGWCC 2 cut(s) 180, 325
BmgBI CACGTC 1 cut(s) 472
BmgT120I GGNCC 2 cut(s) 180, 325
BmiI GGNNCC 1 cut(s) 327
BmrFI CCNGG 1 cut(s) 453
BmsI GCATC 1 cut(s) 139
BpiI GAAGAC 1 cut(s) 536
BplI GAGNNNNNCTC 2 cut(s) 147, 179
Bpu10I CCTNAGC 1 cut(s) 630
BpuMI CCSGG 1 cut(s) 453
BsaHI GRCGYC 1 cut(s) 398
BsaJI CCNNGG 3 cut(s) 138, 401, 452
Bsc4I CCNNNNNNNGG 2 cut(s) 95, 251
Bse1I ACTGG 1 cut(s) 74
Bse3DI GCAATG 1 cut(s) 359
BseDI CCNNGG 3 cut(s) 138, 401, 452
BseGI GGATG 2 cut(s) 321, 462
BseLI CCNNNNNNNGG 2 cut(s) 95, 251
BseMI GCAATG 1 cut(s) 359
BseMII CTCAG 3 cut(s) 20, 504, 621
BseNI ACTGG 1 cut(s) 74
BseRI GAGGAG 1 cut(s) 632
BseXI GCAGC 2 cut(s) 220, 301
BseYI CCCAGC 2 cut(s) 216, 238
BsgI GTGCAG 1 cut(s) 308
BshFI GGCC 3 cut(s) 137, 335, 524
BsiSI CCGG 2 cut(s) 452, 599
BslFI GGGAC 1 cut(s) 405
BslI CCNNNNNNNGG 2 cut(s) 95, 251
BsmAI GTCTC 2 cut(s) 9, 150
BsmFI GGGAC 1 cut(s) 405
BsnI GGCC 3 cut(s) 137, 335, 524
Bsp143I GATC 3 cut(s) 262, 408, 621
Bsp19I CCATGG 2 cut(s) 138, 401
BspACI CCGC 5 cut(s) 99, 183, 345, 356, 521
BspANI GGCC 3 cut(s) 137, 335, 524
BspCNI CTCAG 3 cut(s) 19, 505, 622
BspLI GGNNCC 1 cut(s) 327
BspPI GGATC 1 cut(s) 270
BsrDI GCAATG 1 cut(s) 359
BsrI ACTGG 1 cut(s) 74
BssECI CCNNGG 3 cut(s) 138, 401, 452
BssMI GATC 3 cut(s) 262, 408, 621
BssNI GRCGYC 1 cut(s) 398
BssSI CACGAG 1 cut(s) 153
BssT1I CCWWGG 2 cut(s) 138, 401
Bst2BI CACGAG 1 cut(s) 153
Bst4CI ACNGT 2 cut(s) 119, 286
BstACI GRCGYC 1 cut(s) 398
BstAPI GCANNNNNTGC 1 cut(s) 149
BstC8I GCNNGC 1 cut(s) 145
BstDEI CTNAG 3 cut(s) 6, 513, 630
BstDSI CCRYGG 2 cut(s) 138, 401
BstF5I GGATG 2 cut(s) 321, 462
BstH2I RGCGCY 1 cut(s) 245
BstHHI GCGC 1 cut(s) 244
BstKTI GATC 3 cut(s) 265, 411, 624
BstMAI GTCTC 2 cut(s) 9, 150
BstMBI GATC 3 cut(s) 262, 408, 621
BstMWI GCNNNNNNNGC 4 cut(s) 149, 239, 361, 518
BstNSI RCATGY 1 cut(s) 451
BstSCI CCNGG 1 cut(s) 451
BstSFI CTRYAG 1 cut(s) 12
BstV1I GCAGC 2 cut(s) 220, 301
BstV2I GAAGAC 1 cut(s) 536
BstXI CCANNNNNNTGG 1 cut(s) 691
BsuRI GGCC 3 cut(s) 137, 335, 524
BtgI CCRYGG 2 cut(s) 138, 401
BtgZI GCGATG 1 cut(s) 162
BtrI CACGTC 1 cut(s) 472
BtsCI GGATG 2 cut(s) 321, 462
Cac8I GCNNGC 1 cut(s) 145
CfoI GCGC 1 cut(s) 244
Cfr13I GGNCC 2 cut(s) 180, 325
CseI GACGC 1 cut(s) 406
Csp6I GTAC 2 cut(s) 349, 382
CspCI CAANNNNNGTGG 2 cut(s) 571, 606
CviAII CATG 4 cut(s) 95, 139, 402, 448
CviQI GTAC 2 cut(s) 349, 382
DdeI CTNAG 3 cut(s) 6, 513, 630
DpnI GATC 3 cut(s) 264, 410, 623
DpnII GATC 3 cut(s) 262, 408, 621
EaeI YGGCCR 1 cut(s) 522
Eco130I CCWWGG 2 cut(s) 138, 401
Eco47I GGWCC 2 cut(s) 180, 325
EcoO109I RGGNCCY 1 cut(s) 325
EcoT14I CCWWGG 2 cut(s) 138, 401
ErhI CCWWGG 2 cut(s) 138, 401
FaeI CATG 4 cut(s) 98, 142, 405, 451
FaiI YATR 6 cut(s) 14, 96, 140, 403, 449, 654
FaqI GGGAC 1 cut(s) 405
FatI CATG 4 cut(s) 94, 138, 401, 447
FauI CCCGC 1 cut(s) 338
Fnu4HI GCNGC 4 cut(s) 234, 290, 356, 522
FokI GGATG 2 cut(s) 328, 469
Fsp4HI GCNGC 4 cut(s) 234, 290, 356, 522
FspBI CTAG 1 cut(s) 222
GlaI GCGC 1 cut(s) 243
GluI GCNGC 4 cut(s) 234, 290, 356, 522
GsaI CCCAGC 2 cut(s) 220, 242
HaeII RGCGCY 1 cut(s) 245
HaeIII GGCC 3 cut(s) 137, 335, 524
HapII CCGG 2 cut(s) 452, 599
HgaI GACGC 1 cut(s) 406
HhaI GCGC 1 cut(s) 244
Hin1I GRCGYC 1 cut(s) 398
Hin1II CATG 4 cut(s) 98, 142, 405, 451
Hin6I GCGC 1 cut(s) 242
HinP1I GCGC 1 cut(s) 242
HinfI GANTC 2 cut(s) 129, 441
HpaII CCGG 2 cut(s) 452, 599
Hpy166II GTNNAC 3 cut(s) 104, 351, 382
Hpy188I TCNGA 1 cut(s) 666
Hpy8I GTNNAC 3 cut(s) 104, 351, 382
HpyAV CCTTC 5 cut(s) 410, 469, 502, 511, 661
HpyCH4III ACNGT 2 cut(s) 119, 286
HpyCH4IV ACGT 2 cut(s) 106, 471
HpyCH4V TGCA 4 cut(s) 51, 152, 175, 289
HpyF10VI GCNNNNNNNGC 4 cut(s) 149, 239, 361, 518
HpyF3I CTNAG 3 cut(s) 6, 513, 630
HpySE526I ACGT 2 cut(s) 106, 471
Hsp92I GRCGYC 1 cut(s) 398
Hsp92II CATG 4 cut(s) 98, 142, 405, 451
HspAI GCGC 1 cut(s) 242
Kzo9I GATC 3 cut(s) 262, 408, 621
LmnI GCTCC 1 cut(s) 195
LpnPI CCDG 9 cut(s) 87, 129, 230, 252, 327, 373, 465, 485, 612
Lsp1109I GCAGC 2 cut(s) 220, 301
LweI GCATC 1 cut(s) 139
MaeI CTAG 1 cut(s) 222
MaeII ACGT 2 cut(s) 106, 471
MaeIII GTNAC 1 cut(s) 676
MalI GATC 3 cut(s) 264, 410, 623
MboI GATC 3 cut(s) 262, 408, 621
MboII GAAGA 2 cut(s) 541, 566
MluCI AATT 2 cut(s) 19, 90
MmeI TCCRAC 2 cut(s) 217, 296
MseI TTAA 2 cut(s) 570, 636
MslI CAYNNNNRTG 1 cut(s) 99
MspI CCGG 2 cut(s) 452, 599
MspR9I CCNGG 1 cut(s) 453
MwoI GCNNNNNNNGC 4 cut(s) 149, 239, 361, 518
NciI CCSGG 1 cut(s) 453
NcoI CCATGG 2 cut(s) 138, 401
NdeII GATC 3 cut(s) 262, 408, 621
NlaIII CATG 4 cut(s) 98, 142, 405, 451
NlaIV GGNNCC 1 cut(s) 327
NspI RCATGY 1 cut(s) 451
PfeI GAWTC 2 cut(s) 129, 441
PflMI CCANNNNNTGG 1 cut(s) 95
PkrI GCNGC 4 cut(s) 235, 291, 357, 523
PpuMI RGGWCCY 1 cut(s) 325
Psp5II RGGWCCY 1 cut(s) 325
PspFI CCCAGC 2 cut(s) 216, 238
PspN4I GGNNCC 1 cut(s) 327
PspPI GGNCC 2 cut(s) 180, 325
PspPPI RGGWCCY 1 cut(s) 325
RsaI GTAC 2 cut(s) 350, 383
RsaNI GTAC 2 cut(s) 349, 382
RseI CAYNNNNRTG 1 cut(s) 99
SaqAI TTAA 2 cut(s) 570, 636
SatI GCNGC 4 cut(s) 234, 290, 356, 522
Sau3AI GATC 3 cut(s) 262, 408, 621
Sau96I GGNCC 2 cut(s) 180, 325
ScrFI CCNGG 1 cut(s) 453
SetI ASST 8 cut(s) 109, 116, 165, 192, 235, 474, 480, 636
SfaNI GCATC 1 cut(s) 139
SfcI CTRYAG 1 cut(s) 12
SinI GGWCC 2 cut(s) 180, 325
SmiMI CAYNNNNRTG 1 cut(s) 99
Sse9I AATT 2 cut(s) 19, 90
SsiI CCGC 5 cut(s) 99, 183, 345, 356, 521
SspI AATATT 1 cut(s) 607
SspMI CTAG 1 cut(s) 222
StyD4I CCNGG 1 cut(s) 451
StyI CCWWGG 2 cut(s) 138, 401
TaaI ACNGT 2 cut(s) 119, 286
TaiI ACGT 2 cut(s) 109, 474
TaqI TCGA 2 cut(s) 165, 444
TasI AATT 2 cut(s) 19, 90
TauI GCSGC 2 cut(s) 358, 524
TfiI GAWTC 2 cut(s) 129, 441
Tru1I TTAA 2 cut(s) 570, 636
Tru9I TTAA 2 cut(s) 570, 636
TseI GCWGC 2 cut(s) 233, 289
TspDTI ATGAA 1 cut(s) 83
TspGWI ACGGA 3 cut(s) 409, 548, 664
Van91I CCANNNNNTGG 1 cut(s) 95
VpaK11BI GGWCC 2 cut(s) 180, 325
XapI RAATTY 1 cut(s) 19
XceI RCATGY 1 cut(s) 451
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.