MD13G1108500.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0015604
Physical Location & Seq
Forward (+)
7793385 .. 7800082
6698 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1108500.v1.1.491

Sequence Viewer

Length: 723 bp
ATGTCTCAGGCCGTATCCAATGTCAGCCCCTTTTTCCCACTGTCATCTGTGAAAACCAGAAATGTGCGATACCCTGTTTTCTCAAAACCAATTAATGGTGGCGGATGTTTTAGCCACAGTAGTAAGAACAGCCTTAAGGCCATGGCAGGCGATGCAAGAGACAACCTTGACCACATGCAGAGGGCCACCAAGCTCCAACAACAGCAGGCCCAGCCTAGAAAGAGAGCTGCCCCAGCGCCGCCCATCGGGTTGTGGGACCGGTTTCCAACGGCCAAGACGGTGCAGCAGATGATGGAGACGATGGAGGGTCTGATGGACGACCCGTTTGCCTACTCCGGCGGGTACAGGACGCCAGTCCCGAGCGAGACGGGCGGGTACAGCAGGGGAAGGACGCCGTGGGAGATCAAAGAAGGTGAGGATGCTTACAAGGTGAGATTCGACATGCCCGGGATGACAAAAGAGGACGTGAAGGTGTGGGTGGAGGAGAAAATGCTGGTTGTGAAGGCCGAGAAGGCGGTCAAGAAGACGGAAAATGGGGCGGTGGTGGAAGAAAACGGGGAGGAGTGGTCAGCCAAGAGCTATGGGAGGTACAGCAGTAGAATAGCGTTGCCGGAGAATATTCAGTTTGAGAAGATTAAGGCTGAGGTTAAAGATGGGGTCTTGTACATTACCATTCCAAAGGCGTCCATGACTTCCAAGATTTTGGACATCAACGTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

241

Amino Acids

26.81

Weight (kDa)

9.16

Isoelectric Point (pI)

42.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 134 - 231 3.1e-22 Hsp20/alpha crystallin family
ArsA_HSP20 PF17886 139 - 224 8e-08 HSP20-like domain found in ArsA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015604)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24850
malus_domestica MD13G1108500.v1.1 MD16G1108600.v1.1
prunus_persica Prupe.1G237800_v2.0.a1
pyrus_communis pycom13g09400 pycom16g09270
rosa_chinensis RchiOBHm_Chr4g0431921
rosa_laevigata RLG00000006875
rosa_multiflora Rmu_sc0004965.1_g000008
rosa_roxburghii Rroxscaffold_5G00373350
rosa_rugosa Rorug04G0253000
rosa_samantha Rh4AG310300 Rh4BG317400 Rh4CG332700 Rh4DG312300
rosa_wichuraiana Rw4G027000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 95
AciI CCGC 6 cut(s) 102, 239, 339, 372, 515, 539
AclI AACGTT 1 cut(s) 714
AcoI YGGCCR 1 cut(s) 270
AcyI GRCGYC 3 cut(s) 350, 392, 683
AfaI GTAC 4 cut(s) 344, 377, 590, 665
AfiI CCNNNNNNNGG 2 cut(s) 95, 245
AflII CTTAAG 1 cut(s) 134
AgeI ACCGGT 1 cut(s) 258
AjiI CACGTC 1 cut(s) 466
AluBI AGCT 3 cut(s) 193, 227, 579
AluI AGCT 3 cut(s) 193, 227, 579
Alw26I GTCTC 4 cut(s) 9, 153, 290, 359
Ama87I CYCGRG 2 cut(s) 358, 446
AoxI GGCC 6 cut(s) 9, 138, 183, 207, 270, 504
ApeKI GCWGC 2 cut(s) 227, 283
ArsI GACNNNNNNTTYG 2 cut(s) 308, 340
AseI ATTAAT 1 cut(s) 93
AsiGI ACCGGT 1 cut(s) 258
AspLEI GCGC 1 cut(s) 238
AspS9I GGNCC 3 cut(s) 183, 208, 256
AsuC2I CCSGG 2 cut(s) 447, 448
AsuHPI GGTGA 2 cut(s) 425, 442
AvaI CYCGRG 2 cut(s) 358, 446
AvaII GGWCC 1 cut(s) 256
BaeI ACNNNNGTAYC 2 cut(s) 580, 613
BbsI GAAGAC 1 cut(s) 530
BbvCI CCTCAGC 1 cut(s) 642
BbvI GCAGC 2 cut(s) 214, 295
BccI CCATC 5 cut(s) 251, 286, 295, 307, 647
BceAI ACGGC 2 cut(s) 285, 379
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BciVI GTATCC 1 cut(s) 25
BcnI CCSGG 2 cut(s) 447, 448
BcoDI GTCTC 4 cut(s) 9, 153, 290, 359
BfaI CTAG 1 cut(s) 216
BfoI RGCGCY 1 cut(s) 239
BfrI CTTAAG 1 cut(s) 134
BfuI GTATCC 1 cut(s) 25
BglI GCCNNNNNGGC 1 cut(s) 512
BisI GCNGC 3 cut(s) 228, 239, 284
BlsI GCNGC 3 cut(s) 229, 240, 285
Bme1390I CCNGG 2 cut(s) 447, 448
Bme18I GGWCC 1 cut(s) 256
BmeT110I CYCGRG 2 cut(s) 358, 446
BmgBI CACGTC 1 cut(s) 466
BmgT120I GGNCC 3 cut(s) 183, 208, 256
BmiI GGNNCC 1 cut(s) 257
BmrFI CCNGG 2 cut(s) 447, 448
BmsI GCATC 2 cut(s) 142, 409
BoxI GACNNNNGTC 1 cut(s) 353
BpiI GAAGAC 1 cut(s) 530
Bpu10I CCTNAGC 1 cut(s) 642
BpuMI CCSGG 2 cut(s) 447, 448
BsaHI GRCGYC 3 cut(s) 350, 392, 683
BsaJI CCNNGG 3 cut(s) 141, 395, 446
BsaWI WCCGGW 1 cut(s) 258
BsaXI ACNNNNNCTCC 2 cut(s) 551, 581
Bsc4I CCNNNNNNNGG 2 cut(s) 95, 245
Bse118I RCCGGY 1 cut(s) 258
Bse1I ACTGG 1 cut(s) 353
BseDI CCNNGG 3 cut(s) 141, 395, 446
BseGI GGATG 3 cut(s) 110, 424, 456
BseLI CCNNNNNNNGG 2 cut(s) 95, 245
BseMII CTCAG 2 cut(s) 20, 633
BseNI ACTGG 1 cut(s) 353
BseRI GAGGAG 2 cut(s) 497, 575
BseXI GCAGC 2 cut(s) 214, 295
BseYI CCCAGC 2 cut(s) 210, 232
BsgI GTGCAG 1 cut(s) 302
BshFI GGCC 6 cut(s) 11, 140, 185, 209, 272, 506
BshTI ACCGGT 1 cut(s) 258
BsiHKCI CYCGRG 2 cut(s) 358, 446
BsiSI CCGG 4 cut(s) 259, 336, 447, 611
BslFI GGGAC 2 cut(s) 269, 341
BslI CCNNNNNNNGG 2 cut(s) 95, 245
BsmAI GTCTC 4 cut(s) 9, 153, 290, 359
BsmBI CGTCTC 2 cut(s) 290, 359
BsmFI GGGAC 2 cut(s) 269, 341
BsnI GGCC 6 cut(s) 11, 140, 185, 209, 272, 506
BsoBI CYCGRG 2 cut(s) 358, 446
Bsp1407I TGTACA 1 cut(s) 663
Bsp143I GATC 1 cut(s) 402
Bsp19I CCATGG 1 cut(s) 141
BspACI CCGC 6 cut(s) 102, 239, 339, 372, 515, 539
BspANI GGCC 6 cut(s) 11, 140, 185, 209, 272, 506
BspCNI CTCAG 2 cut(s) 19, 634
BspLI GGNNCC 1 cut(s) 257
BspTI CTTAAG 1 cut(s) 134
BsrFI RCCGGY 1 cut(s) 258
BsrGI TGTACA 1 cut(s) 663
BsrI ACTGG 1 cut(s) 353
BssAI RCCGGY 1 cut(s) 258
BssECI CCNNGG 3 cut(s) 141, 395, 446
BssMI GATC 1 cut(s) 402
BssNI GRCGYC 3 cut(s) 350, 392, 683
BssT1I CCWWGG 1 cut(s) 141
Bst4CI ACNGT 3 cut(s) 42, 119, 280
BstACI GRCGYC 3 cut(s) 350, 392, 683
BstAFI CTTAAG 1 cut(s) 134
BstAPI GCANNNNNTGC 1 cut(s) 152
BstAUI TGTACA 1 cut(s) 663
BstC8I GCNNGC 2 cut(s) 148, 207
BstDEI CTNAG 2 cut(s) 6, 642
BstDSI CCRYGG 2 cut(s) 141, 395
BstF5I GGATG 3 cut(s) 110, 424, 456
BstH2I RGCGCY 1 cut(s) 239
BstHHI GCGC 1 cut(s) 238
BstKTI GATC 1 cut(s) 405
BstMAI GTCTC 4 cut(s) 9, 153, 290, 359
BstMBI GATC 1 cut(s) 402
BstMWI GCNNNNNNNGC 6 cut(s) 152, 211, 233, 369, 378, 512
BstNSI RCATGY 2 cut(s) 178, 445
BstPAI GACNNNNGTC 1 cut(s) 353
BstSCI CCNGG 2 cut(s) 445, 446
BstV1I GCAGC 2 cut(s) 214, 295
BstV2I GAAGAC 1 cut(s) 530
BstXI CCANNNNNNTGG 1 cut(s) 703
BsuI GTATCC 1 cut(s) 25
BsuRI GGCC 6 cut(s) 11, 140, 185, 209, 272, 506
BtgI CCRYGG 2 cut(s) 141, 395
BtgZI GCGATG 1 cut(s) 165
BtrI CACGTC 1 cut(s) 466
BtsCI GGATG 3 cut(s) 110, 424, 456
BtsIMutI CAGTG 1 cut(s) 38
Cac8I GCNNGC 2 cut(s) 148, 207
CfoI GCGC 1 cut(s) 238
Cfr10I RCCGGY 1 cut(s) 258
Cfr13I GGNCC 3 cut(s) 183, 208, 256
Cfr9I CCCGGG 1 cut(s) 446
CseI GACGC 3 cut(s) 358, 400, 672
Csp6I GTAC 4 cut(s) 343, 376, 589, 664
CspAI ACCGGT 1 cut(s) 258
CviAII CATG 4 cut(s) 142, 175, 442, 688
CviQI GTAC 4 cut(s) 343, 376, 589, 664
DdeI CTNAG 2 cut(s) 6, 642
DpnI GATC 1 cut(s) 404
DpnII GATC 1 cut(s) 402
EaeI YGGCCR 1 cut(s) 270
EciI GGCGGA 1 cut(s) 117
Eco130I CCWWGG 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 256
Eco88I CYCGRG 2 cut(s) 358, 446
EcoT14I CCWWGG 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 141
Esp3I CGTCTC 2 cut(s) 290, 359
FaeI CATG 4 cut(s) 145, 178, 445, 691
FaiI YATR 5 cut(s) 143, 176, 443, 582, 689
FaqI GGGAC 2 cut(s) 269, 341
FatI CATG 4 cut(s) 141, 174, 441, 687
FauI CCCGC 2 cut(s) 332, 365
Fnu4HI GCNGC 3 cut(s) 228, 239, 284
FokI GGATG 3 cut(s) 117, 431, 463
Fsp4HI GCNGC 3 cut(s) 228, 239, 284
FspBI CTAG 1 cut(s) 216
GlaI GCGC 1 cut(s) 237
GluI GCNGC 3 cut(s) 228, 239, 284
GsaI CCCAGC 2 cut(s) 214, 236
HaeII RGCGCY 1 cut(s) 239
HaeIII GGCC 6 cut(s) 11, 140, 185, 209, 272, 506
HapII CCGG 4 cut(s) 259, 336, 447, 611
HgaI GACGC 3 cut(s) 358, 400, 672
HhaI GCGC 1 cut(s) 238
Hin1I GRCGYC 3 cut(s) 350, 392, 683
Hin1II CATG 4 cut(s) 145, 178, 445, 691
Hin6I GCGC 1 cut(s) 236
HinP1I GCGC 1 cut(s) 236
HinfI GANTC 1 cut(s) 435
HpaII CCGG 4 cut(s) 259, 336, 447, 611
HphI GGTGA 2 cut(s) 425, 442
Hpy188I TCNGA 1 cut(s) 312
Hpy188III TCNNGA 2 cut(s) 358, 520
HpyAV CCTTC 5 cut(s) 381, 404, 463, 496, 505
HpyCH4III ACNGT 3 cut(s) 42, 119, 280
HpyCH4IV ACGT 2 cut(s) 465, 714
HpyCH4V TGCA 3 cut(s) 155, 178, 283
HpyF10VI GCNNNNNNNGC 6 cut(s) 152, 211, 233, 369, 378, 512
HpyF3I CTNAG 2 cut(s) 6, 642
HpySE526I ACGT 2 cut(s) 465, 714
Hsp92I GRCGYC 3 cut(s) 350, 392, 683
Hsp92II CATG 4 cut(s) 145, 178, 445, 691
HspAI GCGC 1 cut(s) 236
Kzo9I GATC 1 cut(s) 402
LmnI GCTCC 1 cut(s) 198
Lsp1109I GCAGC 2 cut(s) 214, 295
LweI GCATC 2 cut(s) 142, 409
MaeI CTAG 1 cut(s) 216
MaeII ACGT 2 cut(s) 465, 714
MalI GATC 1 cut(s) 404
MboI GATC 1 cut(s) 402
MboII GAAGA 3 cut(s) 535, 560, 643
MluCI AATT 1 cut(s) 90
MmeI TCCRAC 2 cut(s) 220, 290
MnlI CCTC 8 cut(s) 174, 298, 409, 454, 475, 553, 579, 637
MseI TTAA 4 cut(s) 93, 135, 636, 648
MspCI CTTAAG 1 cut(s) 134
MspI CCGG 4 cut(s) 259, 336, 447, 611
MspR9I CCNGG 2 cut(s) 447, 448
MwoI GCNNNNNNNGC 6 cut(s) 152, 211, 233, 369, 378, 512
NciI CCSGG 2 cut(s) 447, 448
NcoI CCATGG 1 cut(s) 141
NdeII GATC 1 cut(s) 402
NlaIII CATG 4 cut(s) 145, 178, 445, 691
NlaIV GGNNCC 1 cut(s) 257
NmeAIII GCCGAG 1 cut(s) 532
NspI RCATGY 2 cut(s) 178, 445
PcsI WCGNNNNNNNCGW 1 cut(s) 356
PfeI GAWTC 1 cut(s) 435
PflMI CCANNNNNTGG 1 cut(s) 95
PinAI ACCGGT 1 cut(s) 258
PkrI GCNGC 3 cut(s) 229, 240, 285
PshAI GACNNNNGTC 1 cut(s) 353
PshBI ATTAAT 1 cut(s) 93
Psp1406I AACGTT 1 cut(s) 714
PspFI CCCAGC 2 cut(s) 210, 232
PspN4I GGNNCC 1 cut(s) 257
PspPI GGNCC 3 cut(s) 183, 208, 256
RsaI GTAC 4 cut(s) 344, 377, 590, 665
RsaNI GTAC 4 cut(s) 343, 376, 589, 664
SaqAI TTAA 4 cut(s) 93, 135, 636, 648
SatI GCNGC 3 cut(s) 228, 239, 284
Sau3AI GATC 1 cut(s) 402
Sau96I GGNCC 3 cut(s) 183, 208, 256
ScrFI CCNGG 2 cut(s) 447, 448
SfaNI GCATC 2 cut(s) 142, 409
SinI GGWCC 1 cut(s) 256
SmaI CCCGGG 1 cut(s) 448
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 1 cut(s) 90
SsiI CCGC 6 cut(s) 102, 239, 339, 372, 515, 539
SspI AATATT 1 cut(s) 619
SspMI CTAG 1 cut(s) 216
StyD4I CCNGG 2 cut(s) 445, 446
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 3 cut(s) 42, 119, 280
TaiI ACGT 2 cut(s) 468, 717
TaqI TCGA 1 cut(s) 438
TasI AATT 1 cut(s) 90
TatI WGTACW 1 cut(s) 663
TauI GCSGC 1 cut(s) 241
TfiI GAWTC 1 cut(s) 435
Tru1I TTAA 4 cut(s) 93, 135, 636, 648
Tru9I TTAA 4 cut(s) 93, 135, 636, 648
TscAI CASTG 1 cut(s) 45
TseI GCWGC 2 cut(s) 227, 283
TspGWI ACGGA 1 cut(s) 542
TspMI CCCGGG 1 cut(s) 446
TspRI CASTG 1 cut(s) 45
Van91I CCANNNNNTGG 1 cut(s) 95
Vha464I CTTAAG 1 cut(s) 134
VpaK11BI GGWCC 1 cut(s) 256
VspI ATTAAT 1 cut(s) 93
XceI RCATGY 2 cut(s) 178, 445
XmaI CCCGGG 1 cut(s) 446
XspI CTAG 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.