Rroxscaffold_5G00373350

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0015604
Physical Location & Seq
Forward (+)
54610382 .. 54611711
1330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00373350.1

Sequence Viewer

Length: 702 bp
ATGTCACAAGCTCTATCCAATTTCAGCTTCTTTCTCCCTTCGTCATCTGTGAAAAGAACCAAAACTCTTCCAATCAATGGTGGAGGTTTGAGCTTGAAGGCCATGGCAAACGAAGCCAGAGACAACCTCGACCACTTGCAGAGAGTCACCAAGCAACCCCAACAACCACAGCAAAACCAATCCAAGAAGAGAGTCGCGCCTACCCCGCCCGTAGGACTATGGGACCGATTTCCAACTGCAAGGACAGTTCAGCAGATGATGGAGACCATGGAGAGGATGATGGACGACCCGCTTGTCTATTCAGGTCAGTCGGGCGTGTGGGCTCCGCCGTTGCCGACCGAGACTGGCGGGTACAGCAGGGGAAGGACACCGTGGGAGATCAAAGAAGCTGAAACAGAGTACAAGATGAGGTTCGACATGCCTGGAATGACCAAAGAGGATGTCAAGGTTTGGGTGGAGGAGAAAATGCTGGTCGTTAAGGCTGAGAAGGCGGTCAAGAAGAAAGATGGTGGTGGAGTCGAGGAAGAAGAACAAGGTGAAGAGTGGTCGGCTAGGAGTTATGGAAAGTATAGTAGTAGAATTGCATTGCCGGAGAATATTCAGTTTGAGAAGATTAAGGCTGAGGTTAAAGATGGGGTTTTGTATATCACTATTCCTAAAGCTATGAGCAGCGCCAAGATTTTGGACATTAATGTTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

233

Amino Acids

26.29

Weight (kDa)

8.89

Isoelectric Point (pI)

58.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 126 - 227 1.4e-23 Hsp20/alpha crystallin family
ArsA_HSP20 PF17886 132 - 217 7.3e-08 HSP20-like domain found in ArsA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015604)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24850
malus_domestica MD13G1108500.v1.1 MD16G1108600.v1.1
prunus_persica Prupe.1G237800_v2.0.a1
pyrus_communis pycom13g09400 pycom16g09270
rosa_chinensis RchiOBHm_Chr4g0431921
rosa_laevigata RLG00000006875
rosa_multiflora Rmu_sc0004965.1_g000008
rosa_roxburghii Rroxscaffold_5G00373350
rosa_rugosa Rorug04G0253000
rosa_samantha Rh4AG310300 Rh4BG317400 Rh4CG332700 Rh4DG312300
rosa_wichuraiana Rw4G027000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 293
AccB7I CCANNNNNTGG 1 cut(s) 77
AccII CGCG 1 cut(s) 197
AciI CCGC 5 cut(s) 206, 290, 326, 348, 491
AfaI GTAC 2 cut(s) 353, 401
AfiI CCNNNNNNNGG 3 cut(s) 77, 212, 273
AgsI TTSAA 2 cut(s) 97, 698
AjnI CCWGG 1 cut(s) 421
AjuI GAANNNNNNNTTGG 2 cut(s) 11, 43
AluBI AGCT 5 cut(s) 11, 27, 93, 389, 662
AluI AGCT 5 cut(s) 11, 27, 93, 389, 662
Alw26I GTCTC 3 cut(s) 114, 257, 335
AoxI GGCC 1 cut(s) 99
ApeKI GCWGC 1 cut(s) 669
ArsI GACNNNNNNTTYG 2 cut(s) 426, 458
AseI ATTAAT 1 cut(s) 690
AspLEI GCGC 2 cut(s) 199, 674
AspS9I GGNCC 1 cut(s) 223
AsuHPI GGTGA 2 cut(s) 139, 548
AvaII GGWCC 1 cut(s) 223
BanII GRGCYC 1 cut(s) 325
BbvCI CCTCAGC 1 cut(s) 621
BbvI GCAGC 1 cut(s) 681
BccI CCATC 4 cut(s) 253, 274, 500, 626
BceAI ACGGC 1 cut(s) 313
BciT130I CCWGG 1 cut(s) 423
BcoDI GTCTC 3 cut(s) 114, 257, 335
BfaI CTAG 1 cut(s) 552
BfoI RGCGCY 1 cut(s) 675
BisI GCNGC 1 cut(s) 670
BlsI GCNGC 1 cut(s) 671
Bme1390I CCNGG 1 cut(s) 423
Bme18I GGWCC 1 cut(s) 223
BmgT120I GGNCC 1 cut(s) 223
BmiI GGNNCC 2 cut(s) 224, 324
BmrFI CCNGG 1 cut(s) 423
BplI GAGNNNNNCTC 2 cut(s) 111, 143
Bpu10I CCTNAGC 1 cut(s) 621
BsaI GGTCTC 1 cut(s) 257
BsaJI CCNNGG 3 cut(s) 102, 267, 371
Bsc4I CCNNNNNNNGG 3 cut(s) 77, 212, 273
Bse1I ACTGG 1 cut(s) 349
Bse3DI GCAATG 1 cut(s) 584
BseBI CCWGG 1 cut(s) 423
BseDI CCNNGG 3 cut(s) 102, 267, 371
BseGI GGATG 2 cut(s) 282, 445
BseLI CCNNNNNNNGG 3 cut(s) 77, 212, 273
BseMI GCAATG 1 cut(s) 584
BseMII CTCAG 2 cut(s) 474, 612
BseNI ACTGG 1 cut(s) 349
BseRI GAGGAG 1 cut(s) 473
BseXI GCAGC 1 cut(s) 681
Bsh1236I CGCG 1 cut(s) 197
Bsh1285I CGRYCG 1 cut(s) 339
BshFI GGCC 1 cut(s) 101
BsiEI CGRYCG 1 cut(s) 339
BsiSI CCGG 1 cut(s) 590
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 3 cut(s) 77, 212, 273
BsmAI GTCTC 3 cut(s) 114, 257, 335
BsmFI GGGAC 1 cut(s) 236
BsnI GGCC 1 cut(s) 101
Bso31I GGTCTC 1 cut(s) 257
Bsp1286I GDGCHC 1 cut(s) 325
Bsp143I GATC 1 cut(s) 378
Bsp19I CCATGG 2 cut(s) 102, 267
BspACI CCGC 5 cut(s) 206, 290, 326, 348, 491
BspANI GGCC 1 cut(s) 101
BspCNI CTCAG 2 cut(s) 475, 613
BspFNI CGCG 1 cut(s) 197
BspLI GGNNCC 2 cut(s) 224, 324
BspTNI GGTCTC 1 cut(s) 257
BsrDI GCAATG 1 cut(s) 584
BsrI ACTGG 1 cut(s) 349
BssECI CCNNGG 3 cut(s) 102, 267, 371
BssMI GATC 1 cut(s) 378
BssT1I CCWWGG 2 cut(s) 102, 267
Bst2UI CCWGG 1 cut(s) 423
Bst4CI ACNGT 2 cut(s) 247, 372
Bst6I CTCTTC 3 cut(s) 72, 182, 534
BstDEI CTNAG 2 cut(s) 483, 621
BstDSI CCRYGG 3 cut(s) 102, 267, 371
BstF5I GGATG 2 cut(s) 282, 445
BstFNI CGCG 1 cut(s) 197
BstH2I RGCGCY 1 cut(s) 675
BstHHI GCGC 2 cut(s) 199, 674
BstKTI GATC 1 cut(s) 381
BstMAI GTCTC 3 cut(s) 114, 257, 335
BstMBI GATC 1 cut(s) 378
BstMCI CGRYCG 1 cut(s) 339
BstMWI GCNNNNNNNGC 4 cut(s) 113, 205, 354, 488
BstNI CCWGG 1 cut(s) 423
BstNSI RCATGY 1 cut(s) 421
BstSCI CCNGG 1 cut(s) 421
BstUI CGCG 1 cut(s) 197
BstV1I GCAGC 1 cut(s) 681
BstXI CCANNNNNNTGG 1 cut(s) 682
BsuRI GGCC 1 cut(s) 101
BtgI CCRYGG 3 cut(s) 102, 267, 371
BtsCI GGATG 2 cut(s) 282, 445
CfoI GCGC 2 cut(s) 199, 674
Cfr13I GGNCC 1 cut(s) 223
Csp6I GTAC 2 cut(s) 352, 400
CviAII CATG 3 cut(s) 103, 268, 418
CviQI GTAC 2 cut(s) 352, 400
DdeI CTNAG 2 cut(s) 483, 621
DpnI GATC 1 cut(s) 380
DpnII GATC 1 cut(s) 378
DrdI GACNNNNNNGTC 1 cut(s) 293
DseDI GACNNNNNNGTC 1 cut(s) 293
Eam1104I CTCTTC 3 cut(s) 72, 182, 534
EarI CTCTTC 3 cut(s) 72, 182, 534
EciI GGCGGA 1 cut(s) 315
Eco130I CCWWGG 2 cut(s) 102, 267
Eco24I GRGCYC 1 cut(s) 325
Eco31I GGTCTC 1 cut(s) 257
Eco47I GGWCC 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 421
EcoT14I CCWWGG 2 cut(s) 102, 267
EcoT38I GRGCYC 1 cut(s) 325
ErhI CCWWGG 2 cut(s) 102, 267
FaeI CATG 3 cut(s) 106, 271, 421
FaiI YATR 8 cut(s) 104, 220, 269, 419, 561, 570, 645, 665
FaqI GGGAC 1 cut(s) 236
FatI CATG 3 cut(s) 102, 267, 417
FauI CCCGC 3 cut(s) 213, 297, 341
Fnu4HI GCNGC 1 cut(s) 670
FokI GGATG 2 cut(s) 289, 452
FriOI GRGCYC 1 cut(s) 325
Fsp4HI GCNGC 1 cut(s) 670
FspBI CTAG 1 cut(s) 552
GlaI GCGC 2 cut(s) 198, 673
GluI GCNGC 1 cut(s) 670
HaeII RGCGCY 1 cut(s) 675
HaeIII GGCC 1 cut(s) 101
HapII CCGG 1 cut(s) 590
HhaI GCGC 2 cut(s) 199, 674
Hin1II CATG 3 cut(s) 106, 271, 421
Hin6I GCGC 2 cut(s) 197, 672
HinP1I GCGC 2 cut(s) 197, 672
HinfI GANTC 3 cut(s) 144, 192, 516
HpaII CCGG 1 cut(s) 590
HphI GGTGA 2 cut(s) 139, 548
Hpy188III TCNNGA 1 cut(s) 496
HpyAV CCTTC 4 cut(s) 48, 91, 357, 481
HpyCH4III ACNGT 2 cut(s) 247, 372
HpyCH4V TGCA 3 cut(s) 139, 239, 584
HpyF10VI GCNNNNNNNGC 4 cut(s) 113, 205, 354, 488
HpyF3I CTNAG 2 cut(s) 483, 621
Hsp92II CATG 3 cut(s) 106, 271, 421
HspAI GCGC 2 cut(s) 197, 672
Kzo9I GATC 1 cut(s) 378
LmnI GCTCC 1 cut(s) 328
LpnPI CCDG 8 cut(s) 130, 288, 330, 343, 408, 435, 455, 603
Lsp1109I GCAGC 1 cut(s) 681
MaeI CTAG 1 cut(s) 552
MaeIII GTNAC 2 cut(s) 3, 145
MalI GATC 1 cut(s) 380
MboI GATC 1 cut(s) 378
MboII GAAGA 7 cut(s) 59, 199, 511, 536, 539, 551, 622
MhlI GDGCHC 1 cut(s) 325
MluCI AATT 2 cut(s) 19, 579
MlyI GAGTC 3 cut(s) 153, 201, 525
MmeI TCCRAC 1 cut(s) 257
MnlI CCTC 8 cut(s) 77, 137, 267, 402, 430, 451, 514, 616
MseI TTAA 4 cut(s) 477, 615, 627, 690
MspI CCGG 1 cut(s) 590
MspR9I CCNGG 1 cut(s) 423
MvaI CCWGG 1 cut(s) 423
MvnI CGCG 1 cut(s) 197
MwoI GCNNNNNNNGC 4 cut(s) 113, 205, 354, 488
NcoI CCATGG 2 cut(s) 102, 267
NdeII GATC 1 cut(s) 378
NlaIII CATG 3 cut(s) 106, 271, 421
NlaIV GGNNCC 2 cut(s) 224, 324
NmuCI GTSAC 2 cut(s) 3, 145
NspI RCATGY 1 cut(s) 421
PflMI CCANNNNNTGG 1 cut(s) 77
PkrI GCNGC 1 cut(s) 671
PleI GAGTC 3 cut(s) 152, 200, 524
PpsI GAGTC 3 cut(s) 152, 200, 524
PshBI ATTAAT 1 cut(s) 690
Psp6I CCWGG 1 cut(s) 421
PspGI CCWGG 1 cut(s) 421
PspN4I GGNNCC 2 cut(s) 224, 324
PspPI GGNCC 1 cut(s) 223
RsaI GTAC 2 cut(s) 353, 401
RsaNI GTAC 2 cut(s) 352, 400
SaqAI TTAA 4 cut(s) 477, 615, 627, 690
SatI GCNGC 1 cut(s) 670
Sau3AI GATC 1 cut(s) 378
Sau96I GGNCC 1 cut(s) 223
SchI GAGTC 3 cut(s) 153, 201, 525
ScrFI CCNGG 1 cut(s) 423
SduI GDGCHC 1 cut(s) 325
SinI GGWCC 1 cut(s) 223
Sse9I AATT 2 cut(s) 19, 579
SsiI CCGC 5 cut(s) 206, 290, 326, 348, 491
SspI AATATT 1 cut(s) 598
SspMI CTAG 1 cut(s) 552
StyD4I CCNGG 1 cut(s) 421
StyI CCWWGG 2 cut(s) 102, 267
TaaI ACNGT 2 cut(s) 247, 372
TaqI TCGA 3 cut(s) 129, 414, 519
TaqII GACCGA 2 cut(s) 240, 353
TasI AATT 2 cut(s) 19, 579
TatI WGTACW 1 cut(s) 399
Tru1I TTAA 4 cut(s) 477, 615, 627, 690
Tru9I TTAA 4 cut(s) 477, 615, 627, 690
TseFI GTSAC 2 cut(s) 3, 145
TseI GCWGC 1 cut(s) 669
Tsp45I GTSAC 2 cut(s) 3, 145
Van91I CCANNNNNTGG 1 cut(s) 77
VpaK11BI GGWCC 1 cut(s) 223
VspI ATTAAT 1 cut(s) 690
XceI RCATGY 1 cut(s) 421
XspI CTAG 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.