FvH4_4g27220

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016317
Physical Location & Seq
Reverse (-)
28308147 .. 28308881
735 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27220.t1

Sequence Viewer

Length: 735 bp
ATGAATTCTGTGAAGATGTTCCATGAAAATGAACAGCGTTCAGTAGCGAGGAGGGAGATGACGATGAGCGGCAACCGACCTTCTCCGTTGAAGATCAACAGAGACTCTCATCTCATTCATAAACAGCAGCCTTCTCGCATGGCCGGAGCTCATCATGGATATCATCAACAGCAGCGGCGCCATCCTGTCATCATTTACACTCAATCTCCAAAAGTCATCCACACACGACCCCACGATTTCATGGCTTTGGTCCAAAAGCTCACCGGCCTCTCGCCCTCAGATAATCAAGACCAAACTCATAACGAATCAGCCGCACACAAAACCCTACATGCTGTTCAAGTTGGGGATCACAATGAAGAAGATAGTGCTGTTGTTGGTAGAGCTGTCAATGATAATGAGACTTCTTCTGGTATGACAGATGAAATTAGTGGCGGTGGTGATAAGGAGGAGAATGTTAATGTTGGTGGTGATGTTCTAGGTGACAGTTCATCTTATAAAGCATTACCAAATCGCGGTTTCAGCGGTTTCAGCCCAATGCAGCAGCAGAATCAGCATCCATATTTTACAGATATTCCAATGTTCACACCGACTTCGGTGGATCATAATCACTTCCTCTGCTCACCTCGCCCCCTTCACAGATTTCCGGATTCGAGCTTGCTGGTATCTCCGAGTACTCTTTGCGGTTCAATATCTCCTTCAATGTTCAAGTTCATCAAGGGACTACCAGAATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VQ

Protein Analysis

245

Amino Acids

27.19

Weight (kDa)

6.78

Isoelectric Point (pI)

60.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 69 - 92 5.5e-11 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016317)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g27220
malus_domestica MD16G1041600.v1.1
prunus_persica Prupe.1G312500_v2.0.a1
pyrus_communis pycom13g03480 pycom16g03600
rosa_chinensis RchiOBHm_Chr4g0435011
rosa_laevigata RLG00000006629
rosa_multiflora Rmu_sc0000400.1_g000033
rosa_roxburghii Rroxscaffold_5G00376200
rosa_rugosa Rorug04G0281600
rosa_samantha Rh4AG337100 Rh4BG345000 Rh4CG359700 Rh4DG339500
rosa_wichuraiana Rw4G029390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 495
AccB1I GGYRCC 1 cut(s) 177
AccBSI CCGCTC 1 cut(s) 69
AccII CGCG 1 cut(s) 513
AccIII TCCGGA 1 cut(s) 643
AciI CCGC 7 cut(s) 69, 175, 312, 432, 513, 522, 681
AclWI GGATC 2 cut(s) 354, 606
AcoI YGGCCR 1 cut(s) 141
AcsI RAATTY 1 cut(s) 4
AcyI GRCGYC 1 cut(s) 178
AfaI GTAC 1 cut(s) 673
AfiI CCNNNNNNNGG 1 cut(s) 512
AgsI TTSAA 5 cut(s) 91, 338, 687, 699, 706
AluBI AGCT 4 cut(s) 149, 259, 383, 654
AluI AGCT 4 cut(s) 149, 259, 383, 654
Alw21I GWGCWC 1 cut(s) 151
Alw26I GTCTC 2 cut(s) 96, 392
AlwI GGATC 2 cut(s) 354, 606
Aor13HI TCCGGA 1 cut(s) 643
AoxI GGCC 2 cut(s) 141, 265
ApeKI GCWGC 4 cut(s) 127, 172, 538, 541
ApoI RAATTY 1 cut(s) 4
Asp700I GAANNNNTTC 1 cut(s) 17
AspLEI GCGC 1 cut(s) 180
AspS9I GGNCC 1 cut(s) 250
AsuHPI GGTGA 5 cut(s) 253, 449, 479, 491, 612
AvaII GGWCC 1 cut(s) 250
BanI GGYRCC 1 cut(s) 177
BanII GRGCYC 1 cut(s) 151
Bbv12I GWGCWC 1 cut(s) 151
BbvI GCAGC 4 cut(s) 139, 184, 550, 553
BccI CCATC 1 cut(s) 189
BcgI CGANNNNNNTGC 2 cut(s) 116, 150
BcoDI GTCTC 2 cut(s) 96, 392
BfaI CTAG 1 cut(s) 476
BfoI RGCGCY 1 cut(s) 181
BisI GCNGC 7 cut(s) 70, 128, 173, 176, 312, 539, 542
BlsI GCNGC 7 cut(s) 71, 129, 174, 177, 313, 540, 543
BmcAI AGTACT 1 cut(s) 673
Bme18I GGWCC 1 cut(s) 250
BmgT120I GGNCC 1 cut(s) 250
BmiI GGNNCC 1 cut(s) 179
BmsI GCATC 1 cut(s) 562
BsaBI GATNNNNATC 1 cut(s) 603
BsaHI GRCGYC 1 cut(s) 178
BsaWI WCCGGW 1 cut(s) 643
BsaXI ACNNNNNCTCC 2 cut(s) 190, 220
Bsc4I CCNNNNNNNGG 1 cut(s) 512
Bse118I RCCGGY 1 cut(s) 263
Bse8I GATNNNNATC 1 cut(s) 603
BseAI TCCGGA 1 cut(s) 643
BseGI GGATG 3 cut(s) 181, 216, 553
BseJI GATNNNNATC 1 cut(s) 603
BseLI CCNNNNNNNGG 1 cut(s) 512
BseMII CTCAG 1 cut(s) 291
BseRI GAGGAG 2 cut(s) 64, 461
BseXI GCAGC 4 cut(s) 139, 184, 550, 553
Bsh1236I CGCG 1 cut(s) 513
BshFI GGCC 2 cut(s) 143, 267
BshNI GGYRCC 1 cut(s) 177
BsiHKAI GWGCWC 1 cut(s) 151
BsiSI CCGG 3 cut(s) 144, 264, 644
BslI CCNNNNNNNGG 1 cut(s) 512
BsmAI GTCTC 2 cut(s) 96, 392
BsnI GGCC 2 cut(s) 143, 267
Bsp1286I GDGCHC 1 cut(s) 151
Bsp13I TCCGGA 1 cut(s) 643
Bsp143I GATC 3 cut(s) 93, 346, 598
BspACI CCGC 7 cut(s) 69, 175, 312, 432, 513, 522, 681
BspANI GGCC 2 cut(s) 143, 267
BspCNI CTCAG 1 cut(s) 290
BspEI TCCGGA 1 cut(s) 643
BspFNI CGCG 1 cut(s) 513
BspLI GGNNCC 1 cut(s) 179
BspPI GGATC 2 cut(s) 354, 606
BspT107I GGYRCC 1 cut(s) 177
BsrBI CCGCTC 1 cut(s) 69
BsrFI RCCGGY 1 cut(s) 263
BssAI RCCGGY 1 cut(s) 263
BssMI GATC 3 cut(s) 93, 346, 598
BssNI GRCGYC 1 cut(s) 178
Bst4CI ACNGT 1 cut(s) 485
BstACI GRCGYC 1 cut(s) 178
BstC8I GCNNGC 1 cut(s) 656
BstDEI CTNAG 1 cut(s) 277
BstF5I GGATG 3 cut(s) 181, 216, 553
BstFNI CGCG 1 cut(s) 513
BstH2I RGCGCY 1 cut(s) 181
BstHHI GCGC 1 cut(s) 180
BstKTI GATC 3 cut(s) 96, 349, 601
BstMAI GTCTC 2 cut(s) 96, 392
BstMBI GATC 3 cut(s) 93, 346, 598
BstMWI GCNNNNNNNGC 4 cut(s) 519, 528, 550, 624
BstNSI RCATGY 1 cut(s) 332
BstUI CGCG 1 cut(s) 513
BstV1I GCAGC 4 cut(s) 139, 184, 550, 553
BsuRI GGCC 2 cut(s) 143, 267
BtsCI GGATG 3 cut(s) 181, 216, 553
Cac8I GCNNGC 1 cut(s) 656
CfoI GCGC 1 cut(s) 180
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 1 cut(s) 250
Csp6I GTAC 1 cut(s) 672
CviAII CATG 5 cut(s) 23, 139, 155, 241, 329
CviQI GTAC 1 cut(s) 672
DdeI CTNAG 1 cut(s) 277
DinI GGCGCC 1 cut(s) 179
DpnI GATC 3 cut(s) 95, 348, 600
DpnII GATC 3 cut(s) 93, 346, 598
EaeI YGGCCR 1 cut(s) 141
Ecl136II GAGCTC 1 cut(s) 149
Eco24I GRGCYC 1 cut(s) 151
Eco32I GATATC 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 250
Eco53kI GAGCTC 1 cut(s) 149
EcoICRI GAGCTC 1 cut(s) 149
EcoRI GAATTC 1 cut(s) 4
EcoRV GATATC 1 cut(s) 161
EcoT38I GRGCYC 1 cut(s) 151
EgeI GGCGCC 1 cut(s) 179
EheI GGCGCC 1 cut(s) 179
FaeI CATG 5 cut(s) 26, 142, 158, 244, 332
FatI CATG 5 cut(s) 22, 138, 154, 240, 328
Fnu4HI GCNGC 7 cut(s) 70, 128, 173, 176, 312, 539, 542
FokI GGATG 3 cut(s) 168, 203, 540
FriOI GRGCYC 1 cut(s) 151
Fsp4HI GCNGC 7 cut(s) 70, 128, 173, 176, 312, 539, 542
FspBI CTAG 1 cut(s) 476
GlaI GCGC 1 cut(s) 179
GluI GCNGC 7 cut(s) 70, 128, 173, 176, 312, 539, 542
HaeII RGCGCY 1 cut(s) 181
HaeIII GGCC 2 cut(s) 143, 267
HapII CCGG 3 cut(s) 144, 264, 644
HhaI GCGC 1 cut(s) 180
Hin1I GRCGYC 1 cut(s) 178
Hin1II CATG 5 cut(s) 26, 142, 158, 244, 332
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HinfI GANTC 4 cut(s) 104, 305, 547, 647
HpaII CCGG 3 cut(s) 144, 264, 644
HphI GGTGA 5 cut(s) 253, 449, 479, 491, 612
Hpy166II GTNNAC 1 cut(s) 582
Hpy188I TCNGA 2 cut(s) 280, 669
Hpy188III TCNNGA 2 cut(s) 287, 644
Hpy8I GTNNAC 1 cut(s) 582
HpyAV CCTTC 4 cut(s) 90, 141, 641, 705
HpyCH4III ACNGT 1 cut(s) 485
HpyCH4V TGCA 1 cut(s) 538
HpyF10VI GCNNNNNNNGC 4 cut(s) 519, 528, 550, 624
HpyF3I CTNAG 1 cut(s) 277
Hsp92I GRCGYC 1 cut(s) 178
Hsp92II CATG 5 cut(s) 26, 142, 158, 244, 332
HspAI GCGC 1 cut(s) 178
KasI GGCGCC 1 cut(s) 177
Kpn2I TCCGGA 1 cut(s) 643
Kzo9I GATC 3 cut(s) 93, 346, 598
LmnI GCTCC 1 cut(s) 146
LpnPI CCDG 6 cut(s) 157, 198, 277, 393, 644, 657
Lsp1109I GCAGC 4 cut(s) 139, 184, 550, 553
LweI GCATC 1 cut(s) 562
MaeI CTAG 1 cut(s) 476
MaeIII GTNAC 1 cut(s) 479
MalI GATC 3 cut(s) 95, 348, 600
MbiI CCGCTC 1 cut(s) 69
MboI GATC 3 cut(s) 93, 346, 598
MboII GAAGA 5 cut(s) 25, 103, 368, 371, 396
MhlI GDGCHC 1 cut(s) 151
MluCI AATT 2 cut(s) 4, 423
Mly113I GGCGCC 1 cut(s) 178
MlyI GAGTC 1 cut(s) 98
MnlI CCTC 7 cut(s) 42, 45, 278, 286, 439, 623, 633
MroI TCCGGA 1 cut(s) 643
MroXI GAANNNNTTC 1 cut(s) 17
MseI TTAA 1 cut(s) 456
MslI CAYNNNNRTG 1 cut(s) 27
MspA1I CMGCKG 2 cut(s) 175, 522
MspI CCGG 3 cut(s) 144, 264, 644
MvnI CGCG 1 cut(s) 513
MwoI GCNNNNNNNGC 4 cut(s) 519, 528, 550, 624
NarI GGCGCC 1 cut(s) 178
NdeII GATC 3 cut(s) 93, 346, 598
NlaIII CATG 5 cut(s) 26, 142, 158, 244, 332
NlaIV GGNNCC 1 cut(s) 179
NmuCI GTSAC 1 cut(s) 479
NspI RCATGY 1 cut(s) 332
PdmI GAANNNNTTC 1 cut(s) 17
PfeI GAWTC 3 cut(s) 305, 547, 647
PkrI GCNGC 7 cut(s) 71, 129, 174, 177, 313, 540, 543
PleI GAGTC 1 cut(s) 98
PluTI GGCGCC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 98
PsiI TTATAA 1 cut(s) 495
Psp124BI GAGCTC 1 cut(s) 151
PspN4I GGNNCC 1 cut(s) 179
PspPI GGNCC 1 cut(s) 250
RsaI GTAC 1 cut(s) 673
RsaNI GTAC 1 cut(s) 672
RseI CAYNNNNRTG 1 cut(s) 27
SacI GAGCTC 1 cut(s) 151
SaqAI TTAA 1 cut(s) 456
SatI GCNGC 7 cut(s) 70, 128, 173, 176, 312, 539, 542
Sau3AI GATC 3 cut(s) 93, 346, 598
Sau96I GGNCC 1 cut(s) 250
ScaI AGTACT 1 cut(s) 673
SchI GAGTC 1 cut(s) 98
SduI GDGCHC 1 cut(s) 151
SetI ASST 7 cut(s) 82, 151, 261, 385, 481, 625, 656
SfaNI GCATC 1 cut(s) 562
SfoI GGCGCC 1 cut(s) 179
SinI GGWCC 1 cut(s) 250
SmiMI CAYNNNNRTG 1 cut(s) 27
Sse9I AATT 2 cut(s) 4, 423
SsiI CCGC 7 cut(s) 69, 175, 312, 432, 513, 522, 681
SspDI GGCGCC 1 cut(s) 177
SspMI CTAG 1 cut(s) 476
SstI GAGCTC 1 cut(s) 151
TaaI ACNGT 1 cut(s) 485
TaqI TCGA 1 cut(s) 650
TasI AATT 2 cut(s) 4, 423
TatI WGTACW 1 cut(s) 671
TauI GCSGC 3 cut(s) 72, 178, 314
TfiI GAWTC 3 cut(s) 305, 547, 647
Tru1I TTAA 1 cut(s) 456
Tru9I TTAA 1 cut(s) 456
TseFI GTSAC 1 cut(s) 479
TseI GCWGC 4 cut(s) 127, 172, 538, 541
Tsp45I GTSAC 1 cut(s) 479
TspDTI ATGAA 9 cut(s) 17, 39, 45, 107, 229, 369, 435, 477, 700
TspGWI ACGGA 1 cut(s) 75
VpaK11BI GGWCC 1 cut(s) 250
XapI RAATTY 1 cut(s) 4
XceI RCATGY 1 cut(s) 332
XmnI GAANNNNTTC 1 cut(s) 17
XspI CTAG 1 cut(s) 476
ZrmI AGTACT 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.