MD16G1041600.v1.1

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016317
Physical Location & Seq
Reverse (-)
2925479 .. 2926249
771 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1041600.v1.1.491

Sequence Viewer

Length: 771 bp
ATGAACCCTGCAAAGTTCCAGCGGGATGATAATCGTCAAGAACAACATTCGTCAGTTAGGAGGGACCAGATGATGATCAACGGAAACCGCCCCTCGCCGTTGAAGATCAACCGAGGCTCCCATATCATCCACAAACCTCCCAATTCCTATCCCCGCATGGGCGCCGCCAATAAGTCAGAGGAGCAACACCACCACCAGAAGCGGCAGCCGGTGATCATCTACACCCAATCTCCGAAAATCATCCACACGCAGGCACGCGATTTCATGGCTTTGGTACAAAAGCTTACCGGCCTCTCCCGCTCCGATAATATTCACGACGGCTTCCAAGGCCAAACCCTAGACACTGACACTACTCATGAAGATCATGCAAAAGTGAAAGTTTCGTCGTGTCAAGACGACCATATCGACAATCCAGACATTGCTAAATCTGCCGTTAGGCGCAGCCATGATGGGAACGAGTCGTGTTCAGATCTGACGGATGAGATTAATTGCGGTGGTGGGAATTACGATAACAATAGTATGATTAATAGTGTTCATCATCAAGTGGGGAGCTCATCGTCTTCCGGATATTCTCCCAATGCCATGTTCAGCAACCCTAGCCCGTATTTTGCAGATATTCCATTGTTTACGCCGACCTCAAATAATTCAGCGGATTTCTTCTGCCCGCCAAGGCCGCTTTACAGATTCTCCGATTCCTCATTGTCTCCGAACGTTGGCGGTTCGATGTCTCCGTCAATGTTCAAGTTCATCAAAGGACTCCCAGAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VQ

Protein Analysis

257

Amino Acids

28.57

Weight (kDa)

6.92

Isoelectric Point (pI)

58.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 77 - 99 9.6e-10 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016317)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g27220
malus_domestica MD16G1041600.v1.1
prunus_persica Prupe.1G312500_v2.0.a1
pyrus_communis pycom13g03480 pycom16g03600
rosa_chinensis RchiOBHm_Chr4g0435011
rosa_laevigata RLG00000006629
rosa_multiflora Rmu_sc0000400.1_g000033
rosa_roxburghii Rroxscaffold_5G00376200
rosa_rugosa Rorug04G0281600
rosa_samantha Rh4AG337100 Rh4BG345000 Rh4CG359700 Rh4DG339500
rosa_wichuraiana Rw4G029390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 161
AccBSI CCGCTC 1 cut(s) 300
AccII CGCG 1 cut(s) 258
AccIII TCCGGA 1 cut(s) 563
AclI AACGTT 1 cut(s) 711
AcyI GRCGYC 1 cut(s) 162
AfaI GTAC 1 cut(s) 276
AfiI CCNNNNNNNGG 3 cut(s) 158, 250, 713
AgsI TTSAA 2 cut(s) 103, 742
AjuI GAANNNNNNNTTGG 2 cut(s) 569, 601
AluBI AGCT 2 cut(s) 283, 552
AluI AGCT 2 cut(s) 283, 552
Alw21I GWGCWC 1 cut(s) 554
Alw26I GTCTC 2 cut(s) 708, 732
Aor13HI TCCGGA 1 cut(s) 563
AoxI GGCC 3 cut(s) 289, 328, 671
ApeKI GCWGC 2 cut(s) 205, 441
AseI ATTAAT 2 cut(s) 486, 525
AspLEI GCGC 2 cut(s) 164, 441
AspS9I GGNCC 1 cut(s) 64
AsuHPI GGTGA 1 cut(s) 223
AvaII GGWCC 1 cut(s) 64
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 1 cut(s) 554
BbsI GAAGAC 1 cut(s) 552
Bbv12I GWGCWC 1 cut(s) 554
BbvI GCAGC 2 cut(s) 217, 453
BccI CCATC 1 cut(s) 443
BceAI ACGGC 3 cut(s) 82, 334, 416
BclI TGATCA 2 cut(s) 75, 213
BcoDI GTCTC 2 cut(s) 708, 732
BfaI CTAG 2 cut(s) 338, 597
BfoI RGCGCY 1 cut(s) 165
BglII AGATCT 1 cut(s) 469
BisI GCNGC 5 cut(s) 165, 203, 206, 442, 674
BlsI GCNGC 5 cut(s) 166, 204, 207, 443, 675
Bme18I GGWCC 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 64
BmiI GGNNCC 3 cut(s) 65, 118, 163
BpiI GAAGAC 1 cut(s) 552
BsaBI GATNNNNATC 2 cut(s) 30, 74
BsaHI GRCGYC 1 cut(s) 162
BsaJI CCNNGG 3 cut(s) 112, 325, 668
BsaWI WCCGGW 1 cut(s) 563
BsaXI ACNNNNNCTCC 6 cut(s) 101, 131, 214, 244, 671, 701
Bsc4I CCNNNNNNNGG 3 cut(s) 158, 250, 713
Bse118I RCCGGY 2 cut(s) 208, 287
Bse3DI GCAATG 1 cut(s) 417
Bse8I GATNNNNATC 2 cut(s) 30, 74
BseAI TCCGGA 1 cut(s) 563
BseDI CCNNGG 3 cut(s) 112, 325, 668
BseGI GGATG 4 cut(s) 31, 126, 240, 484
BseJI GATNNNNATC 2 cut(s) 30, 74
BseLI CCNNNNNNNGG 3 cut(s) 158, 250, 713
BseMI GCAATG 1 cut(s) 417
BseRI GAGGAG 1 cut(s) 194
BseXI GCAGC 2 cut(s) 217, 453
Bsh1236I CGCG 1 cut(s) 258
BshFI GGCC 3 cut(s) 291, 330, 673
BshNI GGYRCC 1 cut(s) 161
BsiHKAI GWGCWC 1 cut(s) 554
BsiSI CCGG 3 cut(s) 209, 288, 564
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 3 cut(s) 158, 250, 713
BsmAI GTCTC 2 cut(s) 708, 732
BsmFI GGGAC 1 cut(s) 77
BsnI GGCC 3 cut(s) 291, 330, 673
Bsp1286I GDGCHC 1 cut(s) 554
Bsp13I TCCGGA 1 cut(s) 563
Bsp143I GATC 5 cut(s) 75, 105, 213, 361, 469
BspANI GGCC 3 cut(s) 291, 330, 673
BspEI TCCGGA 1 cut(s) 563
BspFNI CGCG 1 cut(s) 258
BspHI TCATGA 1 cut(s) 355
BspLI GGNNCC 3 cut(s) 65, 118, 163
BspT107I GGYRCC 1 cut(s) 161
BsrBI CCGCTC 1 cut(s) 300
BsrDI GCAATG 1 cut(s) 417
BsrFI RCCGGY 2 cut(s) 208, 287
BssAI RCCGGY 2 cut(s) 208, 287
BssECI CCNNGG 3 cut(s) 112, 325, 668
BssMI GATC 5 cut(s) 75, 105, 213, 361, 469
BssNI GRCGYC 1 cut(s) 162
BssT1I CCWWGG 2 cut(s) 325, 668
BstACI GRCGYC 1 cut(s) 162
BstC8I GCNNGC 3 cut(s) 252, 256, 665
BstF5I GGATG 4 cut(s) 31, 126, 240, 484
BstFNI CGCG 1 cut(s) 258
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 2 cut(s) 164, 441
BstKTI GATC 5 cut(s) 78, 108, 216, 364, 472
BstMAI GTCTC 2 cut(s) 708, 732
BstMBI GATC 5 cut(s) 75, 105, 213, 361, 469
BstMWI GCNNNNNNNGC 5 cut(s) 297, 327, 428, 597, 673
BstUI CGCG 1 cut(s) 258
BstV1I GCAGC 2 cut(s) 217, 453
BstV2I GAAGAC 1 cut(s) 552
BstX2I RGATCY 1 cut(s) 469
BstYI RGATCY 1 cut(s) 469
BsuRI GGCC 3 cut(s) 291, 330, 673
BtsCI GGATG 4 cut(s) 31, 126, 240, 484
BtsIMutI CAGTG 1 cut(s) 342
Cac8I GCNNGC 3 cut(s) 252, 256, 665
CciI TCATGA 1 cut(s) 355
CfoI GCGC 2 cut(s) 164, 441
Cfr10I RCCGGY 2 cut(s) 208, 287
Cfr13I GGNCC 1 cut(s) 64
Csp6I GTAC 1 cut(s) 275
CviAII CATG 6 cut(s) 157, 265, 356, 365, 446, 583
CviQI GTAC 1 cut(s) 275
DinI GGCGCC 1 cut(s) 163
DpnI GATC 5 cut(s) 77, 107, 215, 363, 471
DpnII GATC 5 cut(s) 75, 105, 213, 361, 469
Ecl136II GAGCTC 1 cut(s) 552
Eco130I CCWWGG 2 cut(s) 325, 668
Eco24I GRGCYC 1 cut(s) 554
Eco47I GGWCC 1 cut(s) 64
Eco53kI GAGCTC 1 cut(s) 552
EcoICRI GAGCTC 1 cut(s) 552
EcoT14I CCWWGG 2 cut(s) 325, 668
EcoT38I GRGCYC 1 cut(s) 554
EgeI GGCGCC 1 cut(s) 163
EheI GGCGCC 1 cut(s) 163
ErhI CCWWGG 2 cut(s) 325, 668
FaeI CATG 6 cut(s) 160, 268, 359, 368, 449, 586
FaiI YATR 9 cut(s) 123, 158, 266, 357, 366, 402, 447, 521, 584
FaqI GGGAC 1 cut(s) 77
FatI CATG 6 cut(s) 156, 264, 355, 364, 445, 582
FauI CCCGC 4 cut(s) 15, 161, 305, 672
FbaI TGATCA 2 cut(s) 75, 213
Fnu4HI GCNGC 5 cut(s) 165, 203, 206, 442, 674
FokI GGATG 4 cut(s) 38, 113, 227, 491
FriOI GRGCYC 1 cut(s) 554
Fsp4HI GCNGC 5 cut(s) 165, 203, 206, 442, 674
FspBI CTAG 2 cut(s) 338, 597
GlaI GCGC 2 cut(s) 163, 440
GluI GCNGC 5 cut(s) 165, 203, 206, 442, 674
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 3 cut(s) 291, 330, 673
HapII CCGG 3 cut(s) 209, 288, 564
HhaI GCGC 2 cut(s) 164, 441
Hin1I GRCGYC 1 cut(s) 162
Hin1II CATG 6 cut(s) 160, 268, 359, 368, 449, 586
Hin6I GCGC 2 cut(s) 162, 439
HinP1I GCGC 2 cut(s) 162, 439
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 4 cut(s) 458, 684, 692, 756
HpaII CCGG 3 cut(s) 209, 288, 564
HphI GGTGA 1 cut(s) 223
Hpy166II GTNNAC 1 cut(s) 627
Hpy188I TCNGA 7 cut(s) 178, 234, 304, 469, 474, 691, 708
Hpy188III TCNNGA 6 cut(s) 38, 314, 356, 392, 413, 564
Hpy8I GTNNAC 1 cut(s) 627
Hpy99I CGWCG 2 cut(s) 320, 388
HpyCH4IV ACGT 1 cut(s) 711
HpyCH4V TGCA 3 cut(s) 11, 368, 611
HpyF10VI GCNNNNNNNGC 5 cut(s) 297, 327, 428, 597, 673
HpySE526I ACGT 1 cut(s) 711
Hsp92I GRCGYC 1 cut(s) 162
Hsp92II CATG 6 cut(s) 160, 268, 359, 368, 449, 586
HspAI GCGC 2 cut(s) 162, 439
KasI GGCGCC 1 cut(s) 161
Kpn2I TCCGGA 1 cut(s) 563
Ksp22I TGATCA 2 cut(s) 75, 213
Kzo9I GATC 5 cut(s) 75, 105, 213, 361, 469
LmnI GCTCC 4 cut(s) 122, 181, 305, 549
LpnPI CCDG 9 cut(s) 21, 32, 80, 209, 222, 236, 301, 426, 577
Lsp1109I GCAGC 2 cut(s) 217, 453
MaeI CTAG 2 cut(s) 338, 597
MaeII ACGT 1 cut(s) 711
MalI GATC 5 cut(s) 77, 107, 215, 363, 471
MbiI CCGCTC 1 cut(s) 300
MboI GATC 5 cut(s) 75, 105, 213, 361, 469
MboII GAAGA 4 cut(s) 115, 371, 552, 649
MflI RGATCY 1 cut(s) 469
MhlI GDGCHC 1 cut(s) 554
MluCI AATT 4 cut(s) 142, 487, 502, 643
Mly113I GGCGCC 1 cut(s) 162
MlyI GAGTC 2 cut(s) 467, 750
MnlI CCTC 8 cut(s) 54, 103, 107, 147, 172, 302, 646, 706
MroI TCCGGA 1 cut(s) 563
MseI TTAA 2 cut(s) 486, 525
MspA1I CMGCKG 2 cut(s) 22, 650
MspI CCGG 3 cut(s) 209, 288, 564
MvnI CGCG 1 cut(s) 258
MwoI GCNNNNNNNGC 5 cut(s) 297, 327, 428, 597, 673
NarI GGCGCC 1 cut(s) 162
NdeII GATC 5 cut(s) 75, 105, 213, 361, 469
NlaIII CATG 6 cut(s) 160, 268, 359, 368, 449, 586
NlaIV GGNNCC 3 cut(s) 65, 118, 163
PagI TCATGA 1 cut(s) 355
PcsI WCGNNNNNNNCGW 2 cut(s) 402, 728
PfeI GAWTC 2 cut(s) 684, 692
PkrI GCNGC 5 cut(s) 166, 204, 207, 443, 675
PleI GAGTC 2 cut(s) 466, 750
PluTI GGCGCC 1 cut(s) 165
PpsI GAGTC 2 cut(s) 466, 750
PshBI ATTAAT 2 cut(s) 486, 525
Psp124BI GAGCTC 1 cut(s) 554
Psp1406I AACGTT 1 cut(s) 711
PspN4I GGNNCC 3 cut(s) 65, 118, 163
PspPI GGNCC 1 cut(s) 64
PsuI RGATCY 1 cut(s) 469
RsaI GTAC 1 cut(s) 276
RsaNI GTAC 1 cut(s) 275
SacI GAGCTC 1 cut(s) 554
SaqAI TTAA 2 cut(s) 486, 525
SatI GCNGC 5 cut(s) 165, 203, 206, 442, 674
Sau3AI GATC 5 cut(s) 75, 105, 213, 361, 469
Sau96I GGNCC 1 cut(s) 64
SchI GAGTC 2 cut(s) 467, 750
SduI GDGCHC 1 cut(s) 554
SetI ASST 5 cut(s) 139, 285, 554, 638, 714
SfoI GGCGCC 1 cut(s) 163
SinI GGWCC 1 cut(s) 64
Sse9I AATT 4 cut(s) 142, 487, 502, 643
SspDI GGCGCC 1 cut(s) 161
SspI AATATT 2 cut(s) 310, 767
SspMI CTAG 2 cut(s) 338, 597
SstI GAGCTC 1 cut(s) 554
StyI CCWWGG 2 cut(s) 325, 668
TaiI ACGT 1 cut(s) 714
TaqI TCGA 2 cut(s) 405, 722
TasI AATT 4 cut(s) 142, 487, 502, 643
TauI GCSGC 3 cut(s) 167, 205, 676
TfiI GAWTC 2 cut(s) 684, 692
Tru1I TTAA 2 cut(s) 486, 525
Tru9I TTAA 2 cut(s) 486, 525
TscAI CASTG 1 cut(s) 349
TseI GCWGC 2 cut(s) 205, 441
TspDTI ATGAA 5 cut(s) 17, 253, 372, 524, 736
TspGWI ACGGA 3 cut(s) 96, 491, 720
TspRI CASTG 1 cut(s) 349
VpaK11BI GGWCC 1 cut(s) 64
VspI ATTAAT 2 cut(s) 486, 525
XspI CTAG 2 cut(s) 338, 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.