FvH4_4g31700

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0018427
Physical Location & Seq
Forward (+)
30739590 .. 30740117
528 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g31700.t1

Sequence Viewer

Length: 528 bp
ATGGTAGAACACTCCAACCTCTCCTACCCTCTTCTTTCCCGAAAGCTGTCGTCGCTCGGAGTTGATCCCTCCCTGCACTCATCCGTAGTGGATCTGATCACATTGAACTGCAAAACGTCCGGATACTGGCAATATTGTAACGTGAGTGGTGGTTGGACGGTTCTTCCTTTGAGCGTTACCATCGAAGAAGCGCACTATATGATTCTCTGCGAGGCTTGCAATGAATTAATCGGACTGGAGGAAGAAGAAGAAGAAGAAGAAGACGACGACAACGATTATGAAGAAGTAATTGGTATGATACCATCGAGTGAGTCGTCGATAAAGGAATTGCTCAAGAGGGTTAGGGTTGGGAACAATGAAAAAGGAATTTGCACTGTTTGTCTCGAAGAATTGAGTAGTACAGGAGGAAAGTCCTATGCTATGGAGATGCCTTGCAGACATTTGTTTCACGACGAGTGTGTTGTGAGATGGCTCAAGGAGAGTCATTATTGCCCAATTTGCCGCTTTGAGATGCCTACTGCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

176

Amino Acids

19.84

Weight (kDa)

4.56

Isoelectric Point (pI)

67.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 124 - 168 2.1e-12 Ring finger domain
zf-C3HC4_2 PF13923 124 - 167 3.9e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 124 - 167 1.5e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 124 - 168 1.9e-07 RING-H2 zinc finger domain
zf-RING_11 PF17123 124 - 154 4.6e-06 RING-like zinc finger
zf-RING_UBOX PF13445 124 - 165 7.1e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018427)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31700
rosa_chinensis RchiOBHm_Chr4g0440231
rosa_laevigata RLG00000006195
rosa_multiflora Rmu_sc0001685.1_g000030
rosa_roxburghii Rroxscaffold_5G00381160
rosa_rugosa Rorug04G0325400
rosa_samantha Rh4AG376100 Rh4CG403800 Rh4DG382900
rosa_wichuraiana Rw4G032190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 119
AciI CCGC 1 cut(s) 502
AclWI GGATC 2 cut(s) 59, 99
AcsI RAATTY 1 cut(s) 366
AfaI GTAC 1 cut(s) 400
AfiI CCNNNNNNNGG 1 cut(s) 126
AgsI TTSAA 1 cut(s) 106
AjuI GAANNNNNNNTTGG 2 cut(s) 273, 305
AluBI AGCT 1 cut(s) 46
AluI AGCT 1 cut(s) 46
Alw26I GTCTC 1 cut(s) 386
AlwI GGATC 2 cut(s) 59, 99
Aor13HI TCCGGA 1 cut(s) 119
ApoI RAATTY 1 cut(s) 366
AseI ATTAAT 1 cut(s) 227
AspLEI GCGC 1 cut(s) 193
BbsI GAAGAC 1 cut(s) 267
BccI CCATC 3 cut(s) 188, 310, 462
BciVI GTATCC 1 cut(s) 116
BclI TGATCA 1 cut(s) 96
BcoDI GTCTC 1 cut(s) 386
BfuI GTATCC 1 cut(s) 116
BisI GCNGC 1 cut(s) 502
BlsI GCNGC 1 cut(s) 503
BmsI GCATC 2 cut(s) 417, 501
BpiI GAAGAC 1 cut(s) 267
BpmI CTGGAG 1 cut(s) 257
BpuEI CTTGAG 2 cut(s) 317, 458
BsaWI WCCGGW 1 cut(s) 119
Bsc4I CCNNNNNNNGG 1 cut(s) 126
Bse1I ACTGG 2 cut(s) 131, 240
Bse3DI GCAATG 1 cut(s) 226
BseAI TCCGGA 1 cut(s) 119
BseGI GGATG 1 cut(s) 80
BseLI CCNNNNNNNGG 1 cut(s) 126
BseMI GCAATG 1 cut(s) 226
BseNI ACTGG 2 cut(s) 131, 240
BsgI GTGCAG 1 cut(s) 59
BsiSI CCGG 1 cut(s) 120
BslI CCNNNNNNNGG 1 cut(s) 126
BsmAI GTCTC 1 cut(s) 386
Bsp13I TCCGGA 1 cut(s) 119
Bsp143I GATC 3 cut(s) 64, 91, 96
BspACI CCGC 1 cut(s) 502
BspEI TCCGGA 1 cut(s) 119
BspPI GGATC 2 cut(s) 59, 99
BsrDI GCAATG 1 cut(s) 226
BsrI ACTGG 2 cut(s) 131, 240
BssMI GATC 3 cut(s) 64, 91, 96
Bst4CI ACNGT 2 cut(s) 160, 376
Bst6I CTCTTC 1 cut(s) 36
BstC8I GCNNGC 1 cut(s) 217
BstF5I GGATG 1 cut(s) 80
BstHHI GCGC 1 cut(s) 193
BstKTI GATC 3 cut(s) 67, 94, 99
BstMAI GTCTC 1 cut(s) 386
BstMBI GATC 3 cut(s) 64, 91, 96
BstMWI GCNNNNNNNGC 3 cut(s) 52, 216, 498
BstV2I GAAGAC 1 cut(s) 267
BstX2I RGATCY 1 cut(s) 91
BstYI RGATCY 1 cut(s) 91
BsuI GTATCC 1 cut(s) 116
BtsCI GGATG 1 cut(s) 80
BtsIMutI CAGTG 1 cut(s) 372
Cac8I GCNNGC 1 cut(s) 217
CfoI GCGC 1 cut(s) 193
Csp6I GTAC 1 cut(s) 399
CviJI RGCY 3 cut(s) 46, 215, 472
CviKI_1 RGCY 3 cut(s) 46, 215, 472
CviQI GTAC 1 cut(s) 399
DpnI GATC 3 cut(s) 66, 93, 98
DpnII GATC 3 cut(s) 64, 91, 96
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
FaiI YATR 7 cut(s) 198, 200, 279, 296, 417, 422, 523
FbaI TGATCA 1 cut(s) 96
Fnu4HI GCNGC 1 cut(s) 502
FokI GGATG 1 cut(s) 67
Fsp4HI GCNGC 1 cut(s) 502
GlaI GCGC 1 cut(s) 192
GluI GCNGC 1 cut(s) 502
GsuI CTGGAG 1 cut(s) 257
HapII CCGG 1 cut(s) 120
HhaI GCGC 1 cut(s) 193
Hin6I GCGC 1 cut(s) 191
HinP1I GCGC 1 cut(s) 191
HinfI GANTC 3 cut(s) 202, 311, 481
HpaII CCGG 1 cut(s) 120
Hpy188I TCNGA 3 cut(s) 59, 96, 233
Hpy188III TCNNGA 5 cut(s) 39, 120, 334, 383, 449
Hpy99I CGWCG 4 cut(s) 55, 269, 319, 455
HpyCH4III ACNGT 2 cut(s) 160, 376
HpyCH4IV ACGT 2 cut(s) 116, 141
HpyCH4V TGCA 6 cut(s) 76, 111, 219, 372, 435, 521
HpyF10VI GCNNNNNNNGC 3 cut(s) 52, 216, 498
HpySE526I ACGT 2 cut(s) 116, 141
HspAI GCGC 1 cut(s) 191
Kpn2I TCCGGA 1 cut(s) 119
Ksp22I TGATCA 1 cut(s) 96
Kzo9I GATC 3 cut(s) 64, 91, 96
LpnPI CCDG 5 cut(s) 86, 112, 133, 221, 387
LweI GCATC 2 cut(s) 417, 501
MaeII ACGT 2 cut(s) 116, 141
MaeIII GTNAC 2 cut(s) 137, 175
MalI GATC 3 cut(s) 66, 93, 98
MboI GATC 3 cut(s) 64, 91, 96
MflI RGATCY 1 cut(s) 91
MluCI AATT 6 cut(s) 224, 288, 326, 366, 389, 495
MlyI GAGTC 2 cut(s) 320, 490
MmeI TCCRAC 2 cut(s) 39, 134
MnlI CCTC 7 cut(s) 29, 39, 79, 205, 232, 330, 398
MroI TCCGGA 1 cut(s) 119
MseI TTAA 1 cut(s) 227
MspI CCGG 1 cut(s) 120
MwoI GCNNNNNNNGC 3 cut(s) 52, 216, 498
NdeII GATC 3 cut(s) 64, 91, 96
PcsI WCGNNNNNNNCGW 2 cut(s) 270, 311
PfeI GAWTC 1 cut(s) 202
PkrI GCNGC 1 cut(s) 503
PleI GAGTC 2 cut(s) 319, 489
PpsI GAGTC 2 cut(s) 319, 489
PshBI ATTAAT 1 cut(s) 227
PsuI RGATCY 1 cut(s) 91
RsaI GTAC 1 cut(s) 400
RsaNI GTAC 1 cut(s) 399
SaqAI TTAA 1 cut(s) 227
SatI GCNGC 1 cut(s) 502
Sau3AI GATC 3 cut(s) 64, 91, 96
SchI GAGTC 2 cut(s) 320, 490
SetI ASST 4 cut(s) 21, 48, 119, 144
SfaNI GCATC 2 cut(s) 417, 501
SmlI CTYRAG 2 cut(s) 332, 473
SmoI CTYRAG 2 cut(s) 332, 473
Sse9I AATT 6 cut(s) 224, 288, 326, 366, 389, 495
SsiI CCGC 1 cut(s) 502
SspI AATATT 1 cut(s) 134
TaaI ACNGT 2 cut(s) 160, 376
TaiI ACGT 2 cut(s) 119, 144
TaqI TCGA 4 cut(s) 183, 305, 317, 384
TasI AATT 6 cut(s) 224, 288, 326, 366, 389, 495
TatI WGTACW 1 cut(s) 398
TauI GCSGC 1 cut(s) 504
TfiI GAWTC 1 cut(s) 202
Tru1I TTAA 1 cut(s) 227
Tru9I TTAA 1 cut(s) 227
TscAI CASTG 1 cut(s) 379
TspDTI ATGAA 3 cut(s) 237, 294, 372
TspGWI ACGGA 1 cut(s) 73
TspRI CASTG 1 cut(s) 379
VspI ATTAAT 1 cut(s) 227
XapI RAATTY 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.