Rw4G032190

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0018427
Physical Location & Seq
Forward (+)
59324293 .. 59324838
546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G032190.1

Sequence Viewer

Length: 546 bp
ATGGTTGATCACTCCAACCTCTCCTACCCTCTTCTTTCCCGGACACTGTCGTCGCTTGGAGTTGACCCCTCGCTGCACTCGTCCGCCGTGGATCTGATCACCTTGAACTGCAAAACGTCCGGCAACTGGCAATATTGTAACATGGGTGGTGGTTGGACGGTTCTGCCCTTGAGAGTTACCATCGAAGAAGCGCACTGCTACATGCTTCACTGCGAGACTTTTTATTGGCTCAGTGAAGTTATGGGCGATTTAATGGAAATGGAAGAAGAAGAAGAAAACCTAGCAAGTGATTTTGAAGATGTAATTGGTATAAAACCTGCGAGTGAGTCGTCGATAAAGGAGTTGTTGAAGAGGGTAAGGGTTGGGAATAACAAAAAAGATGTCTGCACTGTTTGTCTCGAAGAATTGAGTACTGCAGGAATAACCTATGCTATGCAGATGCCTTGCAAACATGTGTTTCACGACGAGTGTGTTGTGAGATGGCTCAAGGAGAGTCATTATTGCCCAGTTTGCCGCTTTGAGATGCCTACTATTGAGAACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

181

Amino Acids

20.58

Weight (kDa)

4.86

Isoelectric Point (pI)

58.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 126 - 172 6.7e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 128 - 172 6.3e-13 Ring finger domain
zf-C3HC4_2 PF13923 129 - 171 4e-10 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 129 - 171 7.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 129 - 169 6.3e-07 RING-type zinc-finger
zf-RING_11 PF17123 129 - 158 1.6e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018427)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31700
rosa_chinensis RchiOBHm_Chr4g0440231
rosa_laevigata RLG00000006195
rosa_multiflora Rmu_sc0001685.1_g000030
rosa_roxburghii Rroxscaffold_5G00381160
rosa_rugosa Rorug04G0325400
rosa_samantha Rh4AG376100 Rh4CG403800 Rh4DG382900
rosa_wichuraiana Rw4G032190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 49
Acc36I ACCTGC 1 cut(s) 325
AciI CCGC 2 cut(s) 84, 514
AclWI GGATC 1 cut(s) 99
AfaI GTAC 1 cut(s) 412
AfiI CCNNNNNNNGG 1 cut(s) 126
AflIII ACRYGT 1 cut(s) 451
AgsI TTSAA 3 cut(s) 106, 296, 349
AjuI GAANNNNNNNTTGG 2 cut(s) 288, 320
Alw26I GTCTC 2 cut(s) 209, 401
AlwI GGATC 1 cut(s) 99
ApeKI GCWGC 1 cut(s) 73
ArsI GACNNNNNNTTYG 2 cut(s) 366, 398
AspLEI GCGC 1 cut(s) 193
AsuC2I CCSGG 1 cut(s) 40
AsuHPI GGTGA 1 cut(s) 91
BbvI GCAGC 1 cut(s) 60
BccI CCATC 2 cut(s) 188, 474
BceAI ACGGC 1 cut(s) 71
BclI TGATCA 2 cut(s) 7, 96
BcnI CCSGG 1 cut(s) 40
BcoDI GTCTC 2 cut(s) 209, 401
BfaI CTAG 1 cut(s) 281
BfmI CTRYAG 1 cut(s) 414
BfuAI ACCTGC 1 cut(s) 325
BisI GCNGC 2 cut(s) 74, 514
BlsI GCNGC 2 cut(s) 75, 515
BmcAI AGTACT 1 cut(s) 412
Bme1390I CCNGG 1 cut(s) 40
BmrFI CCNGG 1 cut(s) 40
BmrI ACTGGG 1 cut(s) 500
BmsI GCATC 2 cut(s) 429, 513
BmuI ACTGGG 1 cut(s) 500
BpuEI CTTGAG 2 cut(s) 190, 470
BpuMI CCSGG 1 cut(s) 40
BsaJI CCNNGG 1 cut(s) 87
Bsc4I CCNNNNNNNGG 1 cut(s) 126
Bse1I ACTGG 2 cut(s) 131, 506
BseDI CCNNGG 1 cut(s) 87
BseLI CCNNNNNNNGG 1 cut(s) 126
BseMII CTCAG 1 cut(s) 244
BseNI ACTGG 2 cut(s) 131, 506
BseXI GCAGC 1 cut(s) 60
BsgI GTGCAG 2 cut(s) 59, 370
BsiSI CCGG 2 cut(s) 40, 120
BslI CCNNNNNNNGG 1 cut(s) 126
BsmAI GTCTC 2 cut(s) 209, 401
Bsp143I GATC 3 cut(s) 7, 91, 96
BspACI CCGC 2 cut(s) 84, 514
BspCNI CTCAG 1 cut(s) 243
BspMAI CTGCAG 1 cut(s) 418
BspMI ACCTGC 1 cut(s) 325
BspPI GGATC 1 cut(s) 99
BsrI ACTGG 2 cut(s) 131, 506
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 3 cut(s) 7, 91, 96
Bst4CI ACNGT 3 cut(s) 48, 160, 391
Bst6I CTCTTC 2 cut(s) 36, 344
BstDEI CTNAG 1 cut(s) 230
BstDSI CCRYGG 1 cut(s) 87
BstHHI GCGC 1 cut(s) 193
BstKTI GATC 3 cut(s) 10, 94, 99
BstMAI GTCTC 2 cut(s) 209, 401
BstMBI GATC 3 cut(s) 7, 91, 96
BstMWI GCNNNNNNNGC 1 cut(s) 510
BstNSI RCATGY 2 cut(s) 205, 455
BstSCI CCNGG 1 cut(s) 38
BstSFI CTRYAG 1 cut(s) 414
BstV1I GCAGC 1 cut(s) 60
BstX2I RGATCY 1 cut(s) 91
BstYI RGATCY 1 cut(s) 91
BtgI CCRYGG 1 cut(s) 87
BtsI GCAGTG 2 cut(s) 193, 208
BtsIMutI CAGTG 5 cut(s) 44, 193, 208, 238, 387
BveI ACCTGC 1 cut(s) 325
CfoI GCGC 1 cut(s) 193
Csp6I GTAC 1 cut(s) 411
CviAII CATG 3 cut(s) 142, 202, 452
CviJI RGCY 2 cut(s) 229, 484
CviKI_1 RGCY 2 cut(s) 229, 484
CviQI GTAC 1 cut(s) 411
DdeI CTNAG 1 cut(s) 230
DpnI GATC 3 cut(s) 9, 93, 98
DpnII GATC 3 cut(s) 7, 91, 96
DrdI GACNNNNNNGTC 1 cut(s) 49
DseDI GACNNNNNNGTC 1 cut(s) 49
Eam1104I CTCTTC 2 cut(s) 36, 344
EarI CTCTTC 2 cut(s) 36, 344
EciI GGCGGA 1 cut(s) 73
FaeI CATG 3 cut(s) 145, 205, 455
FaiI YATR 7 cut(s) 143, 203, 242, 311, 429, 434, 453
FatI CATG 3 cut(s) 141, 201, 451
FbaI TGATCA 2 cut(s) 7, 96
Fnu4HI GCNGC 2 cut(s) 74, 514
Fsp4HI GCNGC 2 cut(s) 74, 514
FspBI CTAG 1 cut(s) 281
GlaI GCGC 1 cut(s) 192
GluI GCNGC 2 cut(s) 74, 514
HapII CCGG 2 cut(s) 40, 120
HhaI GCGC 1 cut(s) 193
Hin1II CATG 3 cut(s) 145, 205, 455
Hin6I GCGC 1 cut(s) 191
HinP1I GCGC 1 cut(s) 191
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HinfI GANTC 2 cut(s) 326, 493
HpaII CCGG 2 cut(s) 40, 120
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 1 cut(s) 64
Hpy188I TCNGA 1 cut(s) 96
Hpy188III TCNNGA 2 cut(s) 398, 461
Hpy8I GTNNAC 1 cut(s) 64
Hpy99I CGWCG 3 cut(s) 55, 334, 467
HpyCH4III ACNGT 3 cut(s) 48, 160, 391
HpyCH4IV ACGT 1 cut(s) 116
HpyCH4V TGCA 6 cut(s) 76, 111, 387, 416, 436, 447
HpyF10VI GCNNNNNNNGC 1 cut(s) 510
HpyF3I CTNAG 1 cut(s) 230
HpySE526I ACGT 1 cut(s) 116
Hsp92II CATG 3 cut(s) 145, 205, 455
HspAI GCGC 1 cut(s) 191
Ksp22I TGATCA 2 cut(s) 7, 96
Kzo9I GATC 3 cut(s) 7, 91, 96
LpnPI CCDG 6 cut(s) 53, 112, 133, 330, 402, 519
Lsp1109I GCAGC 1 cut(s) 60
LweI GCATC 2 cut(s) 429, 513
MaeI CTAG 1 cut(s) 281
MaeII ACGT 1 cut(s) 116
MaeIII GTNAC 2 cut(s) 137, 175
MalI GATC 3 cut(s) 9, 93, 98
MboI GATC 3 cut(s) 7, 91, 96
MboII GAAGA 9 cut(s) 23, 197, 275, 278, 281, 284, 308, 361, 413
MflI RGATCY 1 cut(s) 91
MluCI AATT 3 cut(s) 303, 404, 541
MlyI GAGTC 2 cut(s) 335, 502
MmeI TCCRAC 2 cut(s) 39, 134
MnlI CCTC 4 cut(s) 29, 39, 79, 345
MseI TTAA 2 cut(s) 251, 544
MspI CCGG 2 cut(s) 40, 120
MspR9I CCNGG 1 cut(s) 40
MwoI GCNNNNNNNGC 1 cut(s) 510
NciI CCSGG 1 cut(s) 40
NdeII GATC 3 cut(s) 7, 91, 96
NlaIII CATG 3 cut(s) 145, 205, 455
NspI RCATGY 2 cut(s) 205, 455
PciI ACATGT 1 cut(s) 451
PcsI WCGNNNNNNNCGW 1 cut(s) 77
PflFI GACNNNGTC 1 cut(s) 46
PfoI TCCNGGA 1 cut(s) 38
PkrI GCNGC 2 cut(s) 75, 515
PleI GAGTC 2 cut(s) 334, 501
PpsI GAGTC 2 cut(s) 334, 501
PscI ACATGT 1 cut(s) 451
PstI CTGCAG 1 cut(s) 418
PsuI RGATCY 1 cut(s) 91
PsyI GACNNNGTC 1 cut(s) 46
RsaI GTAC 1 cut(s) 412
RsaNI GTAC 1 cut(s) 411
SaqAI TTAA 2 cut(s) 251, 544
SatI GCNGC 2 cut(s) 74, 514
Sau3AI GATC 3 cut(s) 7, 91, 96
ScaI AGTACT 1 cut(s) 412
SchI GAGTC 2 cut(s) 335, 502
ScrFI CCNGG 1 cut(s) 40
SetI ASST 6 cut(s) 21, 104, 119, 282, 319, 428
SfaNI GCATC 2 cut(s) 429, 513
SfcI CTRYAG 1 cut(s) 414
SmlI CTYRAG 2 cut(s) 169, 485
SmoI CTYRAG 2 cut(s) 169, 485
Sse9I AATT 3 cut(s) 303, 404, 541
SsiI CCGC 2 cut(s) 84, 514
SspI AATATT 1 cut(s) 134
SspMI CTAG 1 cut(s) 281
StyD4I CCNGG 1 cut(s) 38
TaaI ACNGT 3 cut(s) 48, 160, 391
TaiI ACGT 1 cut(s) 119
TaqI TCGA 3 cut(s) 183, 332, 399
TasI AATT 3 cut(s) 303, 404, 541
TatI WGTACW 1 cut(s) 410
TauI GCSGC 1 cut(s) 516
Tru1I TTAA 2 cut(s) 251, 544
Tru9I TTAA 2 cut(s) 251, 544
TscAI CASTG 5 cut(s) 51, 200, 215, 238, 394
TseI GCWGC 1 cut(s) 73
TspRI CASTG 5 cut(s) 51, 200, 215, 238, 394
Tth111I GACNNNGTC 1 cut(s) 46
XceI RCATGY 2 cut(s) 205, 455
XspI CTAG 1 cut(s) 281
ZrmI AGTACT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.