Rh4DG382900

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0018427
Physical Location & Seq
Forward (+)
60033493 .. 60034292
800 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG382900.1

Sequence Viewer

Length: 708 bp
ATGGGGCAGCTCAGTTTTCGCGTCGAGCCTCTAATCGACACGCAACTTCTACTACACGATCAGAAACCTAATTCACCTTCAGTGCCAGAACTGCCACCTCAAATTTTCATCCAATTCGTTCACAAACAAGAGATAAAGCAGGAAGTGAACACACAATTTGATCTGTGGATGGTAGATCACTCCAACCTCTCCTACCCTCTTCTTTCCCGGACACTGTCGTCGCTTGGAGTTGACCCCTCGCTGCACTCGTCCGCCGTGGATCTGATCACCTTGAACTGCAAAACGTCCGGCTACTGGCAATATTGTAACATGAGTGGTGGTTGGACGGTTCTGCCCTTGAGAGTTACCATCGAAGAAGTGCACTGCTACATGCTTCACTGCGAGACTTTTTATTGGCCCAGTGAAGTTATGGGCGATTTAATGGAACTGGAAGAAGAAGAAGAAAACCTAGCAAGTGATTATGAAGATGTAATTGGTATAAAACCTGCGAGTGAGTCGTCGATAAAGGAGTTGTTGACGAGGGTAAGGGTTGGGAATAACAAAAAAGATGTGTGCACTGTTTGTCTCGAAGAATTGAGTACTGCAGGAATATCCTATGCTATGCAGATGCCTTCCAAACATGTGTTTCACGACGAGTGTGTTGTGAGATGGCTCAAGGAGAGTCATTATTGCCCAGTTTGCCGCTTTGAGATGCCTACTATATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

235

Amino Acids

26.99

Weight (kDa)

4.86

Isoelectric Point (pI)

60.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 183 - 228 1.2e-06 RING-H2 zinc finger domain
zf-RING_2 PF13639 184 - 228 5e-10 Ring finger domain
zf-C3HC4_2 PF13923 185 - 227 3.5e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 185 - 227 9.1e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018427)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31700
rosa_chinensis RchiOBHm_Chr4g0440231
rosa_laevigata RLG00000006195
rosa_multiflora Rmu_sc0001685.1_g000030
rosa_roxburghii Rroxscaffold_5G00381160
rosa_rugosa Rorug04G0325400
rosa_samantha Rh4AG376100 Rh4CG403800 Rh4DG382900
rosa_wichuraiana Rw4G032190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 217
Acc36I ACCTGC 1 cut(s) 493
AccII CGCG 1 cut(s) 21
AciI CCGC 2 cut(s) 252, 682
AclWI GGATC 1 cut(s) 267
AcsI RAATTY 1 cut(s) 102
AcuI CTGAAG 1 cut(s) 63
AfaI GTAC 1 cut(s) 580
AfiI CCNNNNNNNGG 1 cut(s) 294
AflIII ACRYGT 1 cut(s) 619
AgsI TTSAA 1 cut(s) 274
AjuI GAANNNNNNNTTGG 2 cut(s) 456, 488
AluBI AGCT 1 cut(s) 10
AluI AGCT 1 cut(s) 10
Alw21I GWGCWC 2 cut(s) 363, 557
Alw26I GTCTC 2 cut(s) 377, 569
Alw44I GTGCAC 2 cut(s) 359, 553
AlwI GGATC 1 cut(s) 267
AoxI GGCC 1 cut(s) 395
ApaLI GTGCAC 2 cut(s) 359, 553
ApeKI GCWGC 2 cut(s) 7, 241
ApoI RAATTY 1 cut(s) 102
AspS9I GGNCC 1 cut(s) 396
AsuC2I CCSGG 1 cut(s) 208
AsuHPI GGTGA 2 cut(s) 66, 259
BaeGI GKGCMC 2 cut(s) 363, 557
Bbv12I GWGCWC 2 cut(s) 363, 557
BbvI GCAGC 2 cut(s) 19, 228
BccI CCATC 3 cut(s) 163, 356, 642
BceAI ACGGC 1 cut(s) 239
BclI TGATCA 1 cut(s) 264
BcnI CCSGG 1 cut(s) 208
BcoDI GTCTC 2 cut(s) 377, 569
BfaI CTAG 1 cut(s) 449
BfmI CTRYAG 1 cut(s) 582
BfuAI ACCTGC 1 cut(s) 493
BisI GCNGC 3 cut(s) 8, 242, 682
BlsI GCNGC 3 cut(s) 9, 243, 683
BmcAI AGTACT 1 cut(s) 580
Bme1390I CCNGG 1 cut(s) 208
BmgT120I GGNCC 1 cut(s) 396
BmrFI CCNGG 1 cut(s) 208
BmrI ACTGGG 2 cut(s) 393, 668
BmsI GCATC 2 cut(s) 597, 681
BmuI ACTGGG 2 cut(s) 393, 668
BpuEI CTTGAG 2 cut(s) 358, 638
BpuMI CCSGG 1 cut(s) 208
BsaJI CCNNGG 1 cut(s) 255
Bsc4I CCNNNNNNNGG 1 cut(s) 294
Bse1I ACTGG 4 cut(s) 299, 399, 432, 674
BseDI CCNNGG 1 cut(s) 255
BseGI GGATG 2 cut(s) 108, 174
BseLI CCNNNNNNNGG 1 cut(s) 294
BseMII CTCAG 1 cut(s) 25
BseNI ACTGG 4 cut(s) 299, 399, 432, 674
BseSI GKGCMC 2 cut(s) 363, 557
BseXI GCAGC 2 cut(s) 19, 228
BsgI GTGCAG 1 cut(s) 227
Bsh1236I CGCG 1 cut(s) 21
BshFI GGCC 1 cut(s) 397
BsiHKAI GWGCWC 2 cut(s) 363, 557
BsiSI CCGG 2 cut(s) 208, 288
BslI CCNNNNNNNGG 1 cut(s) 294
BsmAI GTCTC 2 cut(s) 377, 569
BsnI GGCC 1 cut(s) 397
Bsp1286I GDGCHC 2 cut(s) 363, 557
Bsp143I GATC 5 cut(s) 58, 160, 175, 259, 264
BspACI CCGC 2 cut(s) 252, 682
BspANI GGCC 1 cut(s) 397
BspCNI CTCAG 1 cut(s) 24
BspFNI CGCG 1 cut(s) 21
BspMAI CTGCAG 1 cut(s) 586
BspMI ACCTGC 1 cut(s) 493
BspPI GGATC 1 cut(s) 267
BsrI ACTGG 4 cut(s) 299, 399, 432, 674
BssECI CCNNGG 1 cut(s) 255
BssMI GATC 5 cut(s) 58, 160, 175, 259, 264
Bst4CI ACNGT 3 cut(s) 216, 328, 559
Bst6I CTCTTC 1 cut(s) 204
BstDEI CTNAG 1 cut(s) 11
BstDSI CCRYGG 1 cut(s) 255
BstF5I GGATG 2 cut(s) 108, 174
BstFNI CGCG 1 cut(s) 21
BstKTI GATC 5 cut(s) 61, 163, 178, 262, 267
BstMAI GTCTC 2 cut(s) 377, 569
BstMBI GATC 5 cut(s) 58, 160, 175, 259, 264
BstMWI GCNNNNNNNGC 2 cut(s) 91, 678
BstNSI RCATGY 2 cut(s) 373, 623
BstSCI CCNGG 1 cut(s) 206
BstSFI CTRYAG 1 cut(s) 582
BstSLI GKGCMC 2 cut(s) 363, 557
BstUI CGCG 1 cut(s) 21
BstV1I GCAGC 2 cut(s) 19, 228
BstX2I RGATCY 1 cut(s) 259
BstYI RGATCY 1 cut(s) 259
BsuRI GGCC 1 cut(s) 397
BtgI CCRYGG 1 cut(s) 255
BtsCI GGATG 2 cut(s) 108, 174
BtsI GCAGTG 2 cut(s) 361, 376
BtsIMutI CAGTG 6 cut(s) 87, 212, 361, 376, 406, 555
BveI ACCTGC 1 cut(s) 493
Cfr13I GGNCC 1 cut(s) 396
CseI GACGC 1 cut(s) 10
Csp6I GTAC 1 cut(s) 579
CviAII CATG 3 cut(s) 310, 370, 620
CviJI RGCY 5 cut(s) 10, 28, 291, 397, 652
CviKI_1 RGCY 5 cut(s) 10, 28, 291, 397, 652
CviQI GTAC 1 cut(s) 579
DdeI CTNAG 1 cut(s) 11
DpnI GATC 5 cut(s) 60, 162, 177, 261, 266
DpnII GATC 5 cut(s) 58, 160, 175, 259, 264
DrdI GACNNNNNNGTC 1 cut(s) 217
DseDI GACNNNNNNGTC 1 cut(s) 217
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
EciI GGCGGA 1 cut(s) 241
Eco57I CTGAAG 1 cut(s) 63
FaeI CATG 3 cut(s) 313, 373, 623
FatI CATG 3 cut(s) 309, 369, 619
FbaI TGATCA 1 cut(s) 264
Fnu4HI GCNGC 3 cut(s) 8, 242, 682
FokI GGATG 2 cut(s) 95, 181
Fsp4HI GCNGC 3 cut(s) 8, 242, 682
FspBI CTAG 1 cut(s) 449
GluI GCNGC 3 cut(s) 8, 242, 682
HaeIII GGCC 1 cut(s) 397
HapII CCGG 2 cut(s) 208, 288
HgaI GACGC 1 cut(s) 10
Hin1II CATG 3 cut(s) 313, 373, 623
HincII GTYRAC 2 cut(s) 232, 516
HindII GTYRAC 2 cut(s) 232, 516
HinfI GANTC 2 cut(s) 494, 661
HpaII CCGG 2 cut(s) 208, 288
HphI GGTGA 2 cut(s) 66, 259
Hpy166II GTNNAC 6 cut(s) 121, 148, 232, 361, 516, 555
Hpy188I TCNGA 2 cut(s) 63, 264
Hpy188III TCNNGA 2 cut(s) 566, 629
Hpy8I GTNNAC 6 cut(s) 121, 148, 232, 361, 516, 555
Hpy99I CGWCG 4 cut(s) 26, 223, 502, 635
HpyAV CCTTC 2 cut(s) 87, 621
HpyCH4III ACNGT 3 cut(s) 216, 328, 559
HpyCH4IV ACGT 1 cut(s) 284
HpyCH4V TGCA 6 cut(s) 244, 279, 361, 555, 584, 604
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 678
HpyF3I CTNAG 1 cut(s) 11
HpySE526I ACGT 1 cut(s) 284
Hsp92II CATG 3 cut(s) 313, 373, 623
Ksp22I TGATCA 1 cut(s) 264
Kzo9I GATC 5 cut(s) 58, 160, 175, 259, 264
Lsp1109I GCAGC 2 cut(s) 19, 228
LweI GCATC 2 cut(s) 597, 681
MaeI CTAG 1 cut(s) 449
MaeII ACGT 1 cut(s) 284
MaeIII GTNAC 2 cut(s) 305, 343
MalI GATC 5 cut(s) 60, 162, 177, 261, 266
MboI GATC 5 cut(s) 58, 160, 175, 259, 264
MboII GAAGA 8 cut(s) 191, 365, 443, 446, 449, 452, 476, 581
MflI RGATCY 1 cut(s) 259
MhlI GDGCHC 2 cut(s) 363, 557
MluCI AATT 6 cut(s) 70, 102, 113, 155, 471, 572
MlyI GAGTC 2 cut(s) 503, 670
MmeI TCCRAC 2 cut(s) 207, 302
MnlI CCTC 6 cut(s) 39, 108, 197, 207, 247, 513
MseI TTAA 1 cut(s) 419
MspI CCGG 2 cut(s) 208, 288
MspR9I CCNGG 1 cut(s) 208
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 2 cut(s) 91, 678
NciI CCSGG 1 cut(s) 208
NdeII GATC 5 cut(s) 58, 160, 175, 259, 264
NlaIII CATG 3 cut(s) 313, 373, 623
NspI RCATGY 2 cut(s) 373, 623
PciI ACATGT 1 cut(s) 619
PcsI WCGNNNNNNNCGW 1 cut(s) 245
PflFI GACNNNGTC 1 cut(s) 214
PfoI TCCNGGA 1 cut(s) 206
PkrI GCNGC 3 cut(s) 9, 243, 683
PleI GAGTC 2 cut(s) 502, 669
PpsI GAGTC 2 cut(s) 502, 669
PscI ACATGT 1 cut(s) 619
PspPI GGNCC 1 cut(s) 396
PstI CTGCAG 1 cut(s) 586
PsuI RGATCY 1 cut(s) 259
PsyI GACNNNGTC 1 cut(s) 214
RsaI GTAC 1 cut(s) 580
RsaNI GTAC 1 cut(s) 579
SaqAI TTAA 1 cut(s) 419
SatI GCNGC 3 cut(s) 8, 242, 682
Sau3AI GATC 5 cut(s) 58, 160, 175, 259, 264
Sau96I GGNCC 1 cut(s) 396
ScaI AGTACT 1 cut(s) 580
SchI GAGTC 2 cut(s) 503, 670
ScrFI CCNGG 1 cut(s) 208
SduI GDGCHC 2 cut(s) 363, 557
SetI ASST 9 cut(s) 12, 70, 79, 100, 189, 272, 287, 450, 487
SfaNI GCATC 2 cut(s) 597, 681
SfcI CTRYAG 1 cut(s) 582
SmlI CTYRAG 2 cut(s) 337, 653
SmoI CTYRAG 2 cut(s) 337, 653
Sse9I AATT 6 cut(s) 70, 102, 113, 155, 471, 572
SsiI CCGC 2 cut(s) 252, 682
SspI AATATT 1 cut(s) 302
SspMI CTAG 1 cut(s) 449
StyD4I CCNGG 1 cut(s) 206
TaaI ACNGT 3 cut(s) 216, 328, 559
TaiI ACGT 1 cut(s) 287
TaqI TCGA 5 cut(s) 24, 36, 351, 500, 567
TasI AATT 6 cut(s) 70, 102, 113, 155, 471, 572
TatI WGTACW 1 cut(s) 578
TauI GCSGC 1 cut(s) 684
Tru1I TTAA 1 cut(s) 419
Tru9I TTAA 1 cut(s) 419
TscAI CASTG 6 cut(s) 87, 219, 368, 383, 406, 562
TseI GCWGC 2 cut(s) 7, 241
TspDTI ATGAA 2 cut(s) 97, 477
TspRI CASTG 6 cut(s) 87, 219, 368, 383, 406, 562
Tth111I GACNNNGTC 1 cut(s) 214
VneI GTGCAC 2 cut(s) 359, 553
XapI RAATTY 1 cut(s) 102
XceI RCATGY 2 cut(s) 373, 623
XcmI CCANNNNNNNNNTGG 1 cut(s) 406
XspI CTAG 1 cut(s) 449
ZrmI AGTACT 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.