Rh4CG403800

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0018427
Physical Location & Seq
Forward (+)
65161279 .. 65162248
970 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG403800.1

Sequence Viewer

Length: 783 bp
ATGGGGCAACTCAGTTTTCGCGTCGAGCCTCTAATCGACACGCAGCTTCTACTACACGATCAGAAACCTAATTCACCTTCAGTGCCAGCACTGCCACCTCAAATTTTCATCCAATTCGTTTACAAAGTGAAACAGCACCTCTTCTTATATCCCAAAAATTCATATACATATATACACTTAATTGATTATGATCAGCAAGAGATAAAGCAGGAAGTGAACACACAATTTGATCTGTGGATGGTAGATCACTCCAACCTCTCCTACCCTCTTCTTTCCCGGACGCTGTCGTCGCTTGGAGTTGACCCCTCGCTGCACTTGTCCGCCGTGGATCTGATCACCTTGAACTGCAAAACGTCCGGCTACTGGCAATATTGTAACATGGGTGGTGGTTGGACGGTTCTGCCTTTGAAAGTTACCATCGAAGAAGTGCACTATTACATGCTTCACTGCGAGACTTGTTATTGGTTCAGTCAAGTTACGAGCGATTTAATGGAACTGGAAGAAGAAGAAGAAAACCTAGCAAGTGATTATGAAGATGTAATTGGTAGGAAACCTGCGAGTGAGTCGTCGATAAAGGAGTTGTTGAAGAGGGTAAGGGTTGGGAATAACAGAAAAGATGTCTGCACTGTTTGTCTCGAAGAATTGAGTACTGCAGGAATATCCTATGCTATGCAGATGCCTTGCAAACATGTGTTTCACGACGAGTGTGTTGTGAGATGGCTCAAGGAGAGTCATTGTTGCCCAGTTTGCCGCTTTGAGATGCCTACTATTGAGAGCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

260

Amino Acids

30.1

Weight (kDa)

5.16

Isoelectric Point (pI)

52.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 205 - 251 1.2e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 207 - 251 1.9e-11 Ring finger domain
zf-C3HC4_2 PF13923 208 - 250 2.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 208 - 237 3.6e-06 RING-like zinc finger
zf-C3HC4 PF00097 208 - 250 1.3e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 208 - 248 4e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018427)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31700
rosa_chinensis RchiOBHm_Chr4g0440231
rosa_laevigata RLG00000006195
rosa_multiflora Rmu_sc0001685.1_g000030
rosa_roxburghii Rroxscaffold_5G00381160
rosa_rugosa Rorug04G0325400
rosa_samantha Rh4AG376100 Rh4CG403800 Rh4DG382900
rosa_wichuraiana Rw4G032190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 286
Acc36I ACCTGC 1 cut(s) 562
AccII CGCG 1 cut(s) 21
AciI CCGC 2 cut(s) 321, 751
AclWI GGATC 1 cut(s) 336
AcsI RAATTY 2 cut(s) 102, 157
AcuI CTGAAG 1 cut(s) 63
AfaI GTAC 1 cut(s) 649
AfiI CCNNNNNNNGG 1 cut(s) 363
AflIII ACRYGT 1 cut(s) 688
AgsI TTSAA 3 cut(s) 343, 409, 586
AjuI GAANNNNNNNTTGG 2 cut(s) 525, 557
AluBI AGCT 1 cut(s) 46
AluI AGCT 1 cut(s) 46
Alw21I GWGCWC 1 cut(s) 432
Alw26I GTCTC 2 cut(s) 446, 638
Alw44I GTGCAC 1 cut(s) 428
AlwI GGATC 1 cut(s) 336
ApaLI GTGCAC 1 cut(s) 428
ApeKI GCWGC 2 cut(s) 43, 310
ApoI RAATTY 2 cut(s) 102, 157
AsuC2I CCSGG 1 cut(s) 277
AsuHPI GGTGA 2 cut(s) 66, 328
BaeGI GKGCMC 1 cut(s) 432
Bbv12I GWGCWC 1 cut(s) 432
BbvI GCAGC 2 cut(s) 55, 297
BccI CCATC 3 cut(s) 232, 425, 711
BceAI ACGGC 1 cut(s) 308
BclI TGATCA 2 cut(s) 190, 333
BcnI CCSGG 1 cut(s) 277
BcoDI GTCTC 2 cut(s) 446, 638
BfaI CTAG 1 cut(s) 518
BfmI CTRYAG 1 cut(s) 651
BfuAI ACCTGC 1 cut(s) 562
BisI GCNGC 3 cut(s) 44, 311, 751
BlsI GCNGC 3 cut(s) 45, 312, 752
BmcAI AGTACT 1 cut(s) 649
Bme1390I CCNGG 1 cut(s) 277
BmrFI CCNGG 1 cut(s) 277
BmrI ACTGGG 1 cut(s) 737
BmsI GCATC 2 cut(s) 666, 750
BmuI ACTGGG 1 cut(s) 737
BpuEI CTTGAG 1 cut(s) 707
BpuMI CCSGG 1 cut(s) 277
BsaBI GATNNNNATC 1 cut(s) 189
BsaJI CCNNGG 1 cut(s) 324
Bsc4I CCNNNNNNNGG 1 cut(s) 363
Bse1I ACTGG 3 cut(s) 368, 501, 743
Bse8I GATNNNNATC 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 324
BseGI GGATG 2 cut(s) 108, 243
BseJI GATNNNNATC 1 cut(s) 189
BseLI CCNNNNNNNGG 1 cut(s) 363
BseMII CTCAG 1 cut(s) 25
BseNI ACTGG 3 cut(s) 368, 501, 743
BseSI GKGCMC 1 cut(s) 432
BseXI GCAGC 2 cut(s) 55, 297
BsgI GTGCAG 2 cut(s) 296, 607
Bsh1236I CGCG 1 cut(s) 21
BsiHKAI GWGCWC 1 cut(s) 432
BsiSI CCGG 2 cut(s) 277, 357
BslI CCNNNNNNNGG 1 cut(s) 363
BsmAI GTCTC 2 cut(s) 446, 638
Bsp1286I GDGCHC 1 cut(s) 432
Bsp143I GATC 6 cut(s) 58, 190, 229, 244, 328, 333
BspACI CCGC 2 cut(s) 321, 751
BspCNI CTCAG 1 cut(s) 24
BspFNI CGCG 1 cut(s) 21
BspMAI CTGCAG 1 cut(s) 655
BspMI ACCTGC 1 cut(s) 562
BspPI GGATC 1 cut(s) 336
BsrI ACTGG 3 cut(s) 368, 501, 743
BssECI CCNNGG 1 cut(s) 324
BssMI GATC 6 cut(s) 58, 190, 229, 244, 328, 333
Bst4CI ACNGT 2 cut(s) 397, 628
Bst6I CTCTTC 3 cut(s) 146, 273, 581
BstC8I GCNNGC 1 cut(s) 87
BstDEI CTNAG 1 cut(s) 11
BstDSI CCRYGG 1 cut(s) 324
BstF5I GGATG 2 cut(s) 108, 243
BstFNI CGCG 1 cut(s) 21
BstKTI GATC 6 cut(s) 61, 193, 232, 247, 331, 336
BstMAI GTCTC 2 cut(s) 446, 638
BstMBI GATC 6 cut(s) 58, 190, 229, 244, 328, 333
BstMWI GCNNNNNNNGC 3 cut(s) 91, 289, 747
BstNSI RCATGY 2 cut(s) 442, 692
BstSCI CCNGG 1 cut(s) 275
BstSFI CTRYAG 1 cut(s) 651
BstSLI GKGCMC 1 cut(s) 432
BstUI CGCG 1 cut(s) 21
BstV1I GCAGC 2 cut(s) 55, 297
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
BtgI CCRYGG 1 cut(s) 324
BtsCI GGATG 2 cut(s) 108, 243
BtsI GCAGTG 2 cut(s) 89, 445
BtsIMutI CAGTG 4 cut(s) 87, 89, 445, 624
BveI ACCTGC 1 cut(s) 562
Cac8I GCNNGC 1 cut(s) 87
CseI GACGC 2 cut(s) 10, 289
Csp6I GTAC 1 cut(s) 648
CviAII CATG 3 cut(s) 379, 439, 689
CviJI RGCY 4 cut(s) 28, 46, 360, 721
CviKI_1 RGCY 4 cut(s) 28, 46, 360, 721
CviQI GTAC 1 cut(s) 648
DdeI CTNAG 1 cut(s) 11
DpnI GATC 6 cut(s) 60, 192, 231, 246, 330, 335
DpnII GATC 6 cut(s) 58, 190, 229, 244, 328, 333
DrdI GACNNNNNNGTC 1 cut(s) 286
DseDI GACNNNNNNGTC 1 cut(s) 286
Eam1104I CTCTTC 3 cut(s) 146, 273, 581
EarI CTCTTC 3 cut(s) 146, 273, 581
EciI GGCGGA 1 cut(s) 310
Eco57I CTGAAG 1 cut(s) 63
FaeI CATG 3 cut(s) 382, 442, 692
FatI CATG 3 cut(s) 378, 438, 688
FbaI TGATCA 2 cut(s) 190, 333
Fnu4HI GCNGC 3 cut(s) 44, 311, 751
FokI GGATG 2 cut(s) 95, 250
Fsp4HI GCNGC 3 cut(s) 44, 311, 751
FspBI CTAG 1 cut(s) 518
GluI GCNGC 3 cut(s) 44, 311, 751
HapII CCGG 2 cut(s) 277, 357
HgaI GACGC 2 cut(s) 10, 289
Hin1II CATG 3 cut(s) 382, 442, 692
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HinfI GANTC 2 cut(s) 563, 730
HpaII CCGG 2 cut(s) 277, 357
HphI GGTGA 2 cut(s) 66, 328
Hpy166II GTNNAC 4 cut(s) 121, 217, 301, 430
Hpy188I TCNGA 2 cut(s) 63, 333
Hpy188III TCNNGA 2 cut(s) 635, 698
Hpy8I GTNNAC 4 cut(s) 121, 217, 301, 430
Hpy99I CGWCG 4 cut(s) 26, 292, 571, 704
HpyAV CCTTC 1 cut(s) 87
HpyCH4III ACNGT 2 cut(s) 397, 628
HpyCH4IV ACGT 1 cut(s) 353
HpyCH4V TGCA 7 cut(s) 313, 348, 430, 624, 653, 673, 684
HpyF10VI GCNNNNNNNGC 3 cut(s) 91, 289, 747
HpyF3I CTNAG 1 cut(s) 11
HpySE526I ACGT 1 cut(s) 353
Hsp92II CATG 3 cut(s) 382, 442, 692
Ksp22I TGATCA 2 cut(s) 190, 333
Kzo9I GATC 6 cut(s) 58, 190, 229, 244, 328, 333
LpnPI CCDG 9 cut(s) 99, 194, 290, 349, 370, 482, 567, 639, 756
Lsp1109I GCAGC 2 cut(s) 55, 297
LweI GCATC 2 cut(s) 666, 750
MaeI CTAG 1 cut(s) 518
MaeII ACGT 1 cut(s) 353
MaeIII GTNAC 3 cut(s) 374, 412, 475
MalI GATC 6 cut(s) 60, 192, 231, 246, 330, 335
MboI GATC 6 cut(s) 58, 190, 229, 244, 328, 333
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 1 cut(s) 432
MluCI AATT 9 cut(s) 70, 102, 113, 157, 180, 224, 540, 641, 778
MlyI GAGTC 2 cut(s) 572, 739
MmeI TCCRAC 2 cut(s) 276, 371
MnlI CCTC 7 cut(s) 39, 108, 149, 266, 276, 316, 582
MseI TTAA 3 cut(s) 179, 488, 781
MspI CCGG 2 cut(s) 277, 357
MspR9I CCNGG 1 cut(s) 277
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 3 cut(s) 91, 289, 747
NciI CCSGG 1 cut(s) 277
NdeII GATC 6 cut(s) 58, 190, 229, 244, 328, 333
NlaIII CATG 3 cut(s) 382, 442, 692
NspI RCATGY 2 cut(s) 442, 692
PciI ACATGT 1 cut(s) 688
PflFI GACNNNGTC 1 cut(s) 283
PfoI TCCNGGA 1 cut(s) 275
PkrI GCNGC 3 cut(s) 45, 312, 752
PleI GAGTC 2 cut(s) 571, 738
PpsI GAGTC 2 cut(s) 571, 738
PscI ACATGT 1 cut(s) 688
PstI CTGCAG 1 cut(s) 655
PsuI RGATCY 1 cut(s) 328
PsyI GACNNNGTC 1 cut(s) 283
RsaI GTAC 1 cut(s) 649
RsaNI GTAC 1 cut(s) 648
SaqAI TTAA 3 cut(s) 179, 488, 781
SatI GCNGC 3 cut(s) 44, 311, 751
Sau3AI GATC 6 cut(s) 58, 190, 229, 244, 328, 333
ScaI AGTACT 1 cut(s) 649
SchI GAGTC 2 cut(s) 572, 739
ScrFI CCNGG 1 cut(s) 277
SduI GDGCHC 1 cut(s) 432
SfaNI GCATC 2 cut(s) 666, 750
SfcI CTRYAG 1 cut(s) 651
SmlI CTYRAG 1 cut(s) 722
SmoI CTYRAG 1 cut(s) 722
Sse9I AATT 9 cut(s) 70, 102, 113, 157, 180, 224, 540, 641, 778
SsiI CCGC 2 cut(s) 321, 751
SspI AATATT 1 cut(s) 371
SspMI CTAG 1 cut(s) 518
StyD4I CCNGG 1 cut(s) 275
TaaI ACNGT 2 cut(s) 397, 628
TaiI ACGT 1 cut(s) 356
TaqI TCGA 5 cut(s) 24, 36, 420, 569, 636
TasI AATT 9 cut(s) 70, 102, 113, 157, 180, 224, 540, 641, 778
TatI WGTACW 1 cut(s) 647
TauI GCSGC 1 cut(s) 753
Tru1I TTAA 3 cut(s) 179, 488, 781
Tru9I TTAA 3 cut(s) 179, 488, 781
TscAI CASTG 4 cut(s) 87, 96, 452, 631
TseI GCWGC 2 cut(s) 43, 310
TspDTI ATGAA 3 cut(s) 97, 150, 546
TspRI CASTG 4 cut(s) 87, 96, 452, 631
Tth111I GACNNNGTC 1 cut(s) 283
VneI GTGCAC 1 cut(s) 428
XapI RAATTY 2 cut(s) 102, 157
XceI RCATGY 2 cut(s) 442, 692
XspI CTAG 1 cut(s) 518
ZrmI AGTACT 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.