RLG00000006195

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0018427
Physical Location & Seq
Reverse (-)
5095634 .. 5096401
768 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006195

Sequence Viewer

Length: 768 bp
ATGGGGCAACTCAGTTTTCGCGTCGAGCCTCTAATCGACACACAACTTCTACTACACGACCAGAAACCTAATTCACCTTCAGTGCCAGCACTGCCGCCTCAAATTTTCATCCAATTCGTTTACAAAGTGAAACAGCACCTCTTCTTATATCCCAAAAATTCATATACATATATACACTTAATTGATTATGATCAGCATGAAGTGAACACACAATTTGATCTGTGGATGGTAGATCACTCCAACCTCTCCTACCCTCTTCTTTCCCGGACGCTGTCGTTGCTTGGAGTTGACCCCTCGCTGCACTCGTCCGCCGTGGATCTGATCACCTTGAACTGCAAAACGTCCGGCTACTGGCAATATTGTAACTTGGGTGGTGGTTGGACGGTTCTGCCCTTGAGAGTTACCATCGCAGAAGTGCACTACATGCTTCACTGCGAGACTTGTTATTGGCTCAGTCAAGTTATGAGCGATTTAATGGAACTGGAAGAAGAAGAAGAAAACCTAGCAAGTGATTATGAAGATGTAATTGGTATGAAACCTGCGAGTGAGTCGTCGATAAAGGAGTTGTTGAAAAGGGTGAGGGTTGGGAATAACAAAAAAGATGTCTGCACTGTTTGTCTCGAAGAATTGAGTACTGCAGGAACATCCTATGCTATGCAGATGCCTTGCAAACATGTGTTTCACGACGAGTGTGTTGTGAGATGGCTCAAGGAGAGTCATTATTGCCCAGTTTGCCGCTTTGAGATGCCTACTATTGAGAACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

256

Amino Acids

29.41

Weight (kDa)

5.22

Isoelectric Point (pI)

51.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 200 - 246 1.5e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 202 - 246 1e-12 Ring finger domain
zf-C3HC4_2 PF13923 203 - 245 7.9e-10 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 203 - 245 1.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 203 - 232 8.3e-07 RING-like zinc finger
zf-RING_UBOX PF13445 203 - 243 1.1e-06 RING-type zinc-finger
zf-RING_5 PF14634 203 - 246 8.9e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018427)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31700
rosa_chinensis RchiOBHm_Chr4g0440231
rosa_laevigata RLG00000006195
rosa_multiflora Rmu_sc0001685.1_g000030
rosa_roxburghii Rroxscaffold_5G00381160
rosa_rugosa Rorug04G0325400
rosa_samantha Rh4AG376100 Rh4CG403800 Rh4DG382900
rosa_wichuraiana Rw4G032190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 547
AccII CGCG 1 cut(s) 21
AciI CCGC 3 cut(s) 95, 309, 736
AclWI GGATC 1 cut(s) 324
AcsI RAATTY 2 cut(s) 102, 157
AcuI CTGAAG 1 cut(s) 63
AfaI GTAC 1 cut(s) 634
AfiI CCNNNNNNNGG 1 cut(s) 351
AflIII ACRYGT 1 cut(s) 673
AgsI TTSAA 2 cut(s) 331, 571
AjuI GAANNNNNNNTTGG 2 cut(s) 510, 542
Alw21I GWGCWC 1 cut(s) 420
Alw26I GTCTC 2 cut(s) 431, 623
Alw44I GTGCAC 1 cut(s) 416
AlwI GGATC 1 cut(s) 324
ApaLI GTGCAC 1 cut(s) 416
ApeKI GCWGC 1 cut(s) 298
ApoI RAATTY 2 cut(s) 102, 157
ArsI GACNNNNNNTTYG 2 cut(s) 588, 620
AsuC2I CCSGG 1 cut(s) 265
AsuHPI GGTGA 3 cut(s) 66, 316, 589
BaeGI GKGCMC 1 cut(s) 420
Bbv12I GWGCWC 1 cut(s) 420
BbvI GCAGC 1 cut(s) 285
BccI CCATC 3 cut(s) 220, 413, 696
BceAI ACGGC 1 cut(s) 296
BclI TGATCA 2 cut(s) 190, 321
BcnI CCSGG 1 cut(s) 265
BcoDI GTCTC 2 cut(s) 431, 623
BfaI CTAG 1 cut(s) 503
BfmI CTRYAG 1 cut(s) 636
BfuAI ACCTGC 1 cut(s) 547
BisI GCNGC 3 cut(s) 95, 299, 736
BlsI GCNGC 3 cut(s) 96, 300, 737
BmcAI AGTACT 1 cut(s) 634
Bme1390I CCNGG 1 cut(s) 265
BmrFI CCNGG 1 cut(s) 265
BmrI ACTGGG 1 cut(s) 722
BmsI GCATC 2 cut(s) 651, 735
BmuI ACTGGG 1 cut(s) 722
BpuEI CTTGAG 2 cut(s) 415, 692
BpuMI CCSGG 1 cut(s) 265
BsaBI GATNNNNATC 1 cut(s) 189
BsaJI CCNNGG 1 cut(s) 312
Bsc4I CCNNNNNNNGG 1 cut(s) 351
Bse1I ACTGG 3 cut(s) 356, 486, 728
Bse8I GATNNNNATC 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 312
BseGI GGATG 3 cut(s) 108, 231, 644
BseJI GATNNNNATC 1 cut(s) 189
BseLI CCNNNNNNNGG 1 cut(s) 351
BseMII CTCAG 2 cut(s) 25, 466
BseNI ACTGG 3 cut(s) 356, 486, 728
BseSI GKGCMC 1 cut(s) 420
BseXI GCAGC 1 cut(s) 285
BsgI GTGCAG 2 cut(s) 284, 592
Bsh1236I CGCG 1 cut(s) 21
BsiHKAI GWGCWC 1 cut(s) 420
BsiSI CCGG 2 cut(s) 265, 345
BslI CCNNNNNNNGG 1 cut(s) 351
BsmAI GTCTC 2 cut(s) 431, 623
Bsp1286I GDGCHC 1 cut(s) 420
Bsp143I GATC 5 cut(s) 190, 217, 232, 316, 321
BspACI CCGC 3 cut(s) 95, 309, 736
BspCNI CTCAG 2 cut(s) 24, 465
BspFNI CGCG 1 cut(s) 21
BspMAI CTGCAG 1 cut(s) 640
BspMI ACCTGC 1 cut(s) 547
BspPI GGATC 1 cut(s) 324
BsrI ACTGG 3 cut(s) 356, 486, 728
BssECI CCNNGG 1 cut(s) 312
BssMI GATC 5 cut(s) 190, 217, 232, 316, 321
Bst4CI ACNGT 2 cut(s) 385, 613
Bst6I CTCTTC 2 cut(s) 146, 261
BstAPI GCANNNNNTGC 1 cut(s) 424
BstC8I GCNNGC 1 cut(s) 87
BstDEI CTNAG 2 cut(s) 11, 452
BstDSI CCRYGG 1 cut(s) 312
BstF5I GGATG 3 cut(s) 108, 231, 644
BstFNI CGCG 1 cut(s) 21
BstKTI GATC 5 cut(s) 193, 220, 235, 319, 324
BstMAI GTCTC 2 cut(s) 431, 623
BstMBI GATC 5 cut(s) 190, 217, 232, 316, 321
BstMWI GCNNNNNNNGC 4 cut(s) 91, 277, 424, 732
BstNSI RCATGY 2 cut(s) 427, 677
BstSCI CCNGG 1 cut(s) 263
BstSFI CTRYAG 1 cut(s) 636
BstSLI GKGCMC 1 cut(s) 420
BstUI CGCG 1 cut(s) 21
BstV1I GCAGC 1 cut(s) 285
BstX2I RGATCY 1 cut(s) 316
BstYI RGATCY 1 cut(s) 316
BtgI CCRYGG 1 cut(s) 312
BtgZI GCGATG 1 cut(s) 391
BtsCI GGATG 3 cut(s) 108, 231, 644
BtsI GCAGTG 2 cut(s) 89, 430
BtsIMutI CAGTG 4 cut(s) 87, 89, 430, 609
BveI ACCTGC 1 cut(s) 547
Cac8I GCNNGC 1 cut(s) 87
CseI GACGC 2 cut(s) 10, 277
Csp6I GTAC 1 cut(s) 633
CviAII CATG 3 cut(s) 197, 424, 674
CviJI RGCY 4 cut(s) 28, 348, 451, 706
CviKI_1 RGCY 4 cut(s) 28, 348, 451, 706
CviQI GTAC 1 cut(s) 633
DdeI CTNAG 2 cut(s) 11, 452
DpnI GATC 5 cut(s) 192, 219, 234, 318, 323
DpnII GATC 5 cut(s) 190, 217, 232, 316, 321
Eam1104I CTCTTC 2 cut(s) 146, 261
EarI CTCTTC 2 cut(s) 146, 261
EciI GGCGGA 1 cut(s) 298
Eco57I CTGAAG 1 cut(s) 63
FaeI CATG 3 cut(s) 200, 427, 677
FatI CATG 3 cut(s) 196, 423, 673
FbaI TGATCA 2 cut(s) 190, 321
Fnu4HI GCNGC 3 cut(s) 95, 299, 736
FokI GGATG 3 cut(s) 95, 238, 631
Fsp4HI GCNGC 3 cut(s) 95, 299, 736
FspBI CTAG 1 cut(s) 503
GluI GCNGC 3 cut(s) 95, 299, 736
HapII CCGG 2 cut(s) 265, 345
HgaI GACGC 2 cut(s) 10, 277
Hin1II CATG 3 cut(s) 200, 427, 677
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HinfI GANTC 2 cut(s) 548, 715
HpaII CCGG 2 cut(s) 265, 345
HphI GGTGA 3 cut(s) 66, 316, 589
Hpy166II GTNNAC 4 cut(s) 121, 205, 289, 418
Hpy188I TCNGA 1 cut(s) 321
Hpy188III TCNNGA 2 cut(s) 620, 683
Hpy8I GTNNAC 4 cut(s) 121, 205, 289, 418
Hpy99I CGWCG 3 cut(s) 26, 556, 689
HpyAV CCTTC 1 cut(s) 87
HpyCH4III ACNGT 2 cut(s) 385, 613
HpyCH4IV ACGT 1 cut(s) 341
HpyCH4V TGCA 7 cut(s) 301, 336, 418, 609, 638, 658, 669
HpyF10VI GCNNNNNNNGC 4 cut(s) 91, 277, 424, 732
HpyF3I CTNAG 2 cut(s) 11, 452
HpySE526I ACGT 1 cut(s) 341
Hsp92II CATG 3 cut(s) 200, 427, 677
Ksp22I TGATCA 2 cut(s) 190, 321
Kzo9I GATC 5 cut(s) 190, 217, 232, 316, 321
LpnPI CCDG 9 cut(s) 74, 99, 278, 337, 358, 467, 552, 624, 741
Lsp1109I GCAGC 1 cut(s) 285
LweI GCATC 2 cut(s) 651, 735
MaeI CTAG 1 cut(s) 503
MaeII ACGT 1 cut(s) 341
MaeIII GTNAC 2 cut(s) 362, 400
MalI GATC 5 cut(s) 192, 219, 234, 318, 323
MboI GATC 5 cut(s) 190, 217, 232, 316, 321
MboII GAAGA 8 cut(s) 133, 248, 497, 500, 503, 506, 530, 635
MflI RGATCY 1 cut(s) 316
MhlI GDGCHC 1 cut(s) 420
MluCI AATT 9 cut(s) 70, 102, 113, 157, 180, 212, 525, 626, 763
MlyI GAGTC 2 cut(s) 557, 724
MmeI TCCRAC 2 cut(s) 264, 359
MnlI CCTC 7 cut(s) 39, 108, 149, 254, 264, 304, 573
MseI TTAA 3 cut(s) 179, 473, 766
MspI CCGG 2 cut(s) 265, 345
MspR9I CCNGG 1 cut(s) 265
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 4 cut(s) 91, 277, 424, 732
NciI CCSGG 1 cut(s) 265
NdeII GATC 5 cut(s) 190, 217, 232, 316, 321
NlaIII CATG 3 cut(s) 200, 427, 677
NspI RCATGY 2 cut(s) 427, 677
PciI ACATGT 1 cut(s) 673
PcsI WCGNNNNNNNCGW 1 cut(s) 302
PflFI GACNNNGTC 1 cut(s) 271
PfoI TCCNGGA 1 cut(s) 263
PkrI GCNGC 3 cut(s) 96, 300, 737
PleI GAGTC 2 cut(s) 556, 723
PpsI GAGTC 2 cut(s) 556, 723
PscI ACATGT 1 cut(s) 673
PstI CTGCAG 1 cut(s) 640
PsuI RGATCY 1 cut(s) 316
PsyI GACNNNGTC 1 cut(s) 271
RsaI GTAC 1 cut(s) 634
RsaNI GTAC 1 cut(s) 633
SaqAI TTAA 3 cut(s) 179, 473, 766
SatI GCNGC 3 cut(s) 95, 299, 736
Sau3AI GATC 5 cut(s) 190, 217, 232, 316, 321
ScaI AGTACT 1 cut(s) 634
SchI GAGTC 2 cut(s) 557, 724
ScrFI CCNGG 1 cut(s) 265
SduI GDGCHC 1 cut(s) 420
SetI ASST 8 cut(s) 70, 79, 141, 246, 329, 344, 504, 541
SfaNI GCATC 2 cut(s) 651, 735
SfcI CTRYAG 1 cut(s) 636
SmlI CTYRAG 2 cut(s) 394, 707
SmoI CTYRAG 2 cut(s) 394, 707
Sse9I AATT 9 cut(s) 70, 102, 113, 157, 180, 212, 525, 626, 763
SsiI CCGC 3 cut(s) 95, 309, 736
SspI AATATT 1 cut(s) 359
SspMI CTAG 1 cut(s) 503
StyD4I CCNGG 1 cut(s) 263
TaaI ACNGT 2 cut(s) 385, 613
TaiI ACGT 1 cut(s) 344
TaqI TCGA 4 cut(s) 24, 36, 554, 621
TasI AATT 9 cut(s) 70, 102, 113, 157, 180, 212, 525, 626, 763
TatI WGTACW 1 cut(s) 632
TauI GCSGC 2 cut(s) 97, 738
Tru1I TTAA 3 cut(s) 179, 473, 766
Tru9I TTAA 3 cut(s) 179, 473, 766
TscAI CASTG 4 cut(s) 87, 96, 437, 616
TseI GCWGC 1 cut(s) 298
TspDTI ATGAA 5 cut(s) 97, 150, 213, 531, 548
TspRI CASTG 4 cut(s) 87, 96, 437, 616
Tth111I GACNNNGTC 1 cut(s) 271
VneI GTGCAC 1 cut(s) 416
XapI RAATTY 2 cut(s) 102, 157
XceI RCATGY 2 cut(s) 427, 677
XspI CTAG 1 cut(s) 503
ZrmI AGTACT 1 cut(s) 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.