FvH4_4g35350

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0018432
Physical Location & Seq
Reverse (-)
32675488 .. 32676263
776 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g35350.t1

Sequence Viewer

Length: 510 bp
ATGAAACACGAGAGCGGACTACGCAAAAAGAAATCTCTCTGTGAGAAATCAATGGTGTTGGTGGCGAACATCATTAAGCTTTCATCTGTTTCTCTTGCTAAGATGAGTTGCGGAACCACGACGACAACAACAACAACAATCAGATCAGTGCAGCCAACAGCAGCTCATTCCGGGCATGAAACTACCAACGTCGTCCATAGTACAAGTACTAAAACTGCTGCATCCACCAAGGTTTTACTAGACATGGAACCTTCTCGGCGTCCGAGAAGAAGCCAGAAAAGGCCAGAGGAGCCAGCTGCTGTCGCTCGAAACGGTCATCATCAATCAAAGCCAGCAAAAGTCGCAGCAGACATCCCTCATCAAGGTTTTACTGCTGTCACGCTTCCATCGACGTTTTTACATGTGAAGCGTGATCATCGTCAAGTACGGCCAGACGATGATGTTGATCGCCGAGCTGAAGAGTTTATTCGCAGAAGGAGGCAAATCATCAACGATGTGAATACCGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

170

Amino Acids

18.84

Weight (kDa)

10.45

Isoelectric Point (pI)

69.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018432)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g35350
pyrus_communis pycom13g07250 pycom16g07220
rosa_laevigata RLG00000005782
rosa_roxburghii Rroxscaffold_5G00385460
rosa_rugosa Rorug04G0356800
rosa_samantha Rh4AG418400 Rh4BG430100 Rh4CG444900 Rh4DG426100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 15
AciI CCGC 2 cut(s) 15, 111
AcoI YGGCCR 1 cut(s) 428
AcuI CTGAAG 1 cut(s) 477
AcyI GRCGYC 1 cut(s) 259
AfaI GTAC 3 cut(s) 202, 208, 426
AfiI CCNNNNNNNGG 1 cut(s) 362
AflIII ACRYGT 1 cut(s) 400
AluBI AGCT 4 cut(s) 79, 164, 296, 455
AluI AGCT 4 cut(s) 79, 164, 296, 455
AlwNI CAGNNNCTG 1 cut(s) 299
AoxI GGCC 2 cut(s) 281, 428
ApeKI GCWGC 5 cut(s) 151, 161, 218, 296, 344
AsuC2I CCSGG 1 cut(s) 172
BauI CACGAG 1 cut(s) 8
BbvI GCAGC 5 cut(s) 163, 173, 205, 283, 356
BccI CCATC 1 cut(s) 394
BceAI ACGGC 1 cut(s) 443
BclI TGATCA 1 cut(s) 412
BcnI CCSGG 1 cut(s) 172
BfaI CTAG 1 cut(s) 239
BisI GCNGC 5 cut(s) 152, 162, 219, 297, 345
BlsI GCNGC 5 cut(s) 153, 163, 220, 298, 346
BmcAI AGTACT 1 cut(s) 208
Bme1390I CCNGG 1 cut(s) 172
BmiI GGNNCC 3 cut(s) 115, 249, 291
BmrFI CCNGG 1 cut(s) 172
BmsI GCATC 1 cut(s) 230
BpuMI CCSGG 1 cut(s) 172
BsaBI GATNNNNATC 1 cut(s) 444
BsaHI GRCGYC 1 cut(s) 259
BsaJI CCNNGG 1 cut(s) 228
Bsc4I CCNNNNNNNGG 1 cut(s) 362
Bse8I GATNNNNATC 1 cut(s) 444
BseDI CCNNGG 1 cut(s) 228
BseGI GGATG 2 cut(s) 221, 351
BseJI GATNNNNATC 1 cut(s) 444
BseLI CCNNNNNNNGG 1 cut(s) 362
BseRI GAGGAG 1 cut(s) 302
BseXI GCAGC 5 cut(s) 163, 173, 205, 283, 356
BsgI GTGCAG 1 cut(s) 170
BshFI GGCC 2 cut(s) 283, 430
BsiSI CCGG 1 cut(s) 171
BslI CCNNNNNNNGG 1 cut(s) 362
BsnI GGCC 2 cut(s) 283, 430
Bsp143I GATC 3 cut(s) 143, 412, 445
BspACI CCGC 2 cut(s) 15, 111
BspANI GGCC 2 cut(s) 283, 430
BspLI GGNNCC 3 cut(s) 115, 249, 291
BsrBI CCGCTC 1 cut(s) 15
BssECI CCNNGG 1 cut(s) 228
BssMI GATC 3 cut(s) 143, 412, 445
BssNI GRCGYC 1 cut(s) 259
BssSI CACGAG 1 cut(s) 8
BssT1I CCWWGG 1 cut(s) 228
Bst2BI CACGAG 1 cut(s) 8
Bst4CI ACNGT 1 cut(s) 314
Bst6I CTCTTC 1 cut(s) 453
BstACI GRCGYC 1 cut(s) 259
BstC8I GCNNGC 2 cut(s) 294, 333
BstDEI CTNAG 1 cut(s) 99
BstENI CCTNNNNNAGG 1 cut(s) 360
BstF5I GGATG 2 cut(s) 221, 351
BstKTI GATC 3 cut(s) 146, 415, 448
BstMBI GATC 3 cut(s) 143, 412, 445
BstMWI GCNNNNNNNGC 4 cut(s) 21, 289, 302, 341
BstNSI RCATGY 1 cut(s) 404
BstSCI CCNGG 1 cut(s) 170
BstV1I GCAGC 5 cut(s) 163, 173, 205, 283, 356
BsuRI GGCC 2 cut(s) 283, 430
BtsCI GGATG 2 cut(s) 221, 351
BtsIMutI CAGTG 1 cut(s) 153
Cac8I GCNNGC 2 cut(s) 294, 333
CaiI CAGNNNCTG 1 cut(s) 299
CseI GACGC 1 cut(s) 248
Csp6I GTAC 3 cut(s) 201, 207, 425
CviAII CATG 3 cut(s) 176, 244, 401
CviQI GTAC 3 cut(s) 201, 207, 425
DdeI CTNAG 1 cut(s) 99
DpnI GATC 3 cut(s) 145, 414, 447
DpnII GATC 3 cut(s) 143, 412, 445
EaeI YGGCCR 1 cut(s) 428
Eam1104I CTCTTC 1 cut(s) 453
EarI CTCTTC 1 cut(s) 453
Eco130I CCWWGG 1 cut(s) 228
Eco57I CTGAAG 1 cut(s) 477
EcoNI CCTNNNNNAGG 1 cut(s) 360
EcoT14I CCWWGG 1 cut(s) 228
ErhI CCWWGG 1 cut(s) 228
FaeI CATG 3 cut(s) 179, 247, 404
FaiI YATR 4 cut(s) 177, 198, 245, 402
FatI CATG 3 cut(s) 175, 243, 400
FbaI TGATCA 1 cut(s) 412
Fnu4HI GCNGC 5 cut(s) 152, 162, 219, 297, 345
FokI GGATG 2 cut(s) 208, 338
Fsp4HI GCNGC 5 cut(s) 152, 162, 219, 297, 345
FspBI CTAG 1 cut(s) 239
GluI GCNGC 5 cut(s) 152, 162, 219, 297, 345
HaeIII GGCC 2 cut(s) 283, 430
HapII CCGG 1 cut(s) 171
HgaI GACGC 1 cut(s) 248
Hin1I GRCGYC 1 cut(s) 259
Hin1II CATG 3 cut(s) 179, 247, 404
HindIII AAGCTT 1 cut(s) 77
HpaII CCGG 1 cut(s) 171
Hpy188I TCNGA 2 cut(s) 143, 264
Hpy99I CGWCG 3 cut(s) 124, 194, 394
HpyAV CCTTC 2 cut(s) 261, 468
HpyCH4III ACNGT 1 cut(s) 314
HpyCH4IV ACGT 2 cut(s) 189, 392
HpyCH4V TGCA 2 cut(s) 151, 221
HpyF10VI GCNNNNNNNGC 4 cut(s) 21, 289, 302, 341
HpyF3I CTNAG 1 cut(s) 99
HpySE526I ACGT 2 cut(s) 189, 392
Hsp92I GRCGYC 1 cut(s) 259
Hsp92II CATG 3 cut(s) 179, 247, 404
Ksp22I TGATCA 1 cut(s) 412
Kzo9I GATC 3 cut(s) 143, 412, 445
LmnI GCTCC 1 cut(s) 289
LpnPI CCDG 6 cut(s) 184, 287, 297, 306, 345, 444
Lsp1109I GCAGC 5 cut(s) 163, 173, 205, 283, 356
LweI GCATC 1 cut(s) 230
MaeI CTAG 1 cut(s) 239
MaeII ACGT 2 cut(s) 189, 392
MaeIII GTNAC 1 cut(s) 376
MalI GATC 3 cut(s) 145, 414, 447
MbiI CCGCTC 1 cut(s) 15
MboI GATC 3 cut(s) 143, 412, 445
MboII GAAGA 2 cut(s) 279, 470
MnlI CCTC 3 cut(s) 280, 366, 471
MseI TTAA 1 cut(s) 75
MspA1I CMGCKG 1 cut(s) 296
MspI CCGG 1 cut(s) 171
MspR9I CCNGG 1 cut(s) 172
MwoI GCNNNNNNNGC 4 cut(s) 21, 289, 302, 341
NciI CCSGG 1 cut(s) 172
NdeII GATC 3 cut(s) 143, 412, 445
NlaIII CATG 3 cut(s) 179, 247, 404
NlaIV GGNNCC 3 cut(s) 115, 249, 291
NmeAIII GCCGAG 2 cut(s) 235, 476
NmuCI GTSAC 1 cut(s) 376
NspI RCATGY 1 cut(s) 404
PciI ACATGT 1 cut(s) 400
PcsI WCGNNNNNNNCGW 1 cut(s) 386
PkrI GCNGC 5 cut(s) 153, 163, 220, 298, 346
PscI ACATGT 1 cut(s) 400
PspN4I GGNNCC 3 cut(s) 115, 249, 291
PstNI CAGNNNCTG 1 cut(s) 299
PvuII CAGCTG 1 cut(s) 296
RsaI GTAC 3 cut(s) 202, 208, 426
RsaNI GTAC 3 cut(s) 201, 207, 425
SaqAI TTAA 1 cut(s) 75
SatI GCNGC 5 cut(s) 152, 162, 219, 297, 345
Sau3AI GATC 3 cut(s) 143, 412, 445
ScaI AGTACT 1 cut(s) 208
ScrFI CCNGG 1 cut(s) 172
SetI ASST 9 cut(s) 81, 166, 192, 234, 253, 298, 367, 395, 457
SfaNI GCATC 1 cut(s) 230
SsiI CCGC 2 cut(s) 15, 111
SspMI CTAG 1 cut(s) 239
StyD4I CCNGG 1 cut(s) 170
StyI CCWWGG 1 cut(s) 228
TaaI ACNGT 1 cut(s) 314
TaiI ACGT 2 cut(s) 192, 395
TaqI TCGA 2 cut(s) 307, 389
TatI WGTACW 2 cut(s) 200, 206
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TscAI CASTG 1 cut(s) 153
TseFI GTSAC 1 cut(s) 376
TseI GCWGC 5 cut(s) 151, 161, 218, 296, 344
Tsp45I GTSAC 1 cut(s) 376
TspDTI ATGAA 3 cut(s) 17, 72, 192
TspRI CASTG 1 cut(s) 153
XagI CCTNNNNNAGG 1 cut(s) 360
XceI RCATGY 1 cut(s) 404
XspI CTAG 1 cut(s) 239
ZrmI AGTACT 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.