Rh4CG444900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0018432
Physical Location & Seq
Reverse (-)
68063416 .. 68064180
765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG444900.1

Sequence Viewer

Length: 492 bp
ATGAGACACAAGAGCGGACTACGCAAAAGGAAATCTCTTTGTGAGAAATCAATGGTTTTGGTGTCGAACATCATTAAGCTTTCATCTGTTTCTCTTGGTAAAATGAGTTGGGGAACCACGACGACGTCGACAACAACAACAATCAGATCAGTGCAGCCAGCAGCAGCTCATTCCGGGCCTGAAACCACCAACGTCGTCAGTCGTACTAGTACTGCTGCTTCCACCGAGGTTTTACTAGACATTGAACCTCCTCAGCGTCCGAGAATAAGCCAGGAAAGGCCAGAGGAACCAGCTGCTGTTGCTCGAAACGATCATCATCAATCAAACCAAGCAAAAGTCACATCAAACATCCCTCATGGTTTTTATACTGTCACTTCTCCGTCTGCACATGTGAAGCGGGATCATCAACTACCACCGGACGATGTTGATGGTCGAGCTGAAGATTTTATTCGAAGGAGGAGGCAAATCATCAACGATGTGAATAGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

163

Amino Acids

18.0

Weight (kDa)

10.08

Isoelectric Point (pI)

67.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018432)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g35350
pyrus_communis pycom13g07250 pycom16g07220
rosa_laevigata RLG00000005782
rosa_roxburghii Rroxscaffold_5G00385460
rosa_rugosa Rorug04G0356800
rosa_samantha Rh4AG418400 Rh4BG430100 Rh4CG444900 Rh4DG426100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 128
AccBSI CCGCTC 1 cut(s) 15
AccI GTMKAC 1 cut(s) 128
AciI CCGC 2 cut(s) 15, 397
AclWI GGATC 1 cut(s) 408
AcuI CTGAAG 1 cut(s) 459
AcyI GRCGYC 1 cut(s) 125
AfaI GTAC 3 cut(s) 205, 211, 487
AflIII ACRYGT 1 cut(s) 388
AgsI TTSAA 1 cut(s) 245
AhlI ACTAGT 1 cut(s) 206
AjnI CCWGG 1 cut(s) 270
AluBI AGCT 4 cut(s) 79, 167, 293, 437
AluI AGCT 4 cut(s) 79, 167, 293, 437
AlwI GGATC 1 cut(s) 408
AlwNI CAGNNNCTG 1 cut(s) 296
AoxI GGCC 2 cut(s) 176, 278
ApeKI GCWGC 5 cut(s) 154, 161, 164, 215, 293
AspS9I GGNCC 1 cut(s) 176
AsuC2I CCSGG 1 cut(s) 175
AsuII TTCGAA 1 cut(s) 451
BarI GAAGNNNNNNTAC 2 cut(s) 202, 234
BbvCI CCTCAGC 1 cut(s) 252
BbvI GCAGC 5 cut(s) 166, 173, 176, 202, 280
BccI CCATC 1 cut(s) 422
BciT130I CCWGG 1 cut(s) 272
BcnI CCSGG 1 cut(s) 175
BcuI ACTAGT 1 cut(s) 206
BfaI CTAG 2 cut(s) 207, 236
BisI GCNGC 5 cut(s) 155, 162, 165, 216, 294
BlsI GCNGC 5 cut(s) 156, 163, 166, 217, 295
BmcAI AGTACT 2 cut(s) 211, 487
Bme1390I CCNGG 2 cut(s) 175, 272
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 2 cut(s) 115, 288
BmrFI CCNGG 2 cut(s) 175, 272
Bpu10I CCTNAGC 1 cut(s) 252
Bpu14I TTCGAA 1 cut(s) 451
BpuMI CCSGG 1 cut(s) 175
BsaBI GATNNNNATC 1 cut(s) 315
BsaHI GRCGYC 1 cut(s) 125
BsaJI CCNNGG 1 cut(s) 225
BsaWI WCCGGW 1 cut(s) 415
Bse8I GATNNNNATC 1 cut(s) 315
BseBI CCWGG 1 cut(s) 272
BseDI CCNNGG 1 cut(s) 225
BseGI GGATG 1 cut(s) 348
BseJI GATNNNNATC 1 cut(s) 315
BseMII CTCAG 1 cut(s) 266
BseRI GAGGAG 2 cut(s) 240, 472
BseXI GCAGC 5 cut(s) 166, 173, 176, 202, 280
BsgI GTGCAG 2 cut(s) 173, 369
BshFI GGCC 2 cut(s) 178, 280
BsiSI CCGG 2 cut(s) 174, 416
BsnI GGCC 2 cut(s) 178, 280
Bsp119I TTCGAA 1 cut(s) 451
Bsp143I GATC 3 cut(s) 146, 310, 400
BspACI CCGC 2 cut(s) 15, 397
BspANI GGCC 2 cut(s) 178, 280
BspCNI CTCAG 1 cut(s) 265
BspLI GGNNCC 2 cut(s) 115, 288
BspPI GGATC 1 cut(s) 408
BspT104I TTCGAA 1 cut(s) 451
BsrBI CCGCTC 1 cut(s) 15
BssECI CCNNGG 1 cut(s) 225
BssMI GATC 3 cut(s) 146, 310, 400
BssNI GRCGYC 1 cut(s) 125
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 1 cut(s) 370
BstACI GRCGYC 1 cut(s) 125
BstBI TTCGAA 1 cut(s) 451
BstC8I GCNNGC 1 cut(s) 159
BstDEI CTNAG 1 cut(s) 252
BstF5I GGATG 1 cut(s) 348
BstKTI GATC 3 cut(s) 149, 313, 403
BstMBI GATC 3 cut(s) 146, 310, 400
BstMWI GCNNNNNNNGC 2 cut(s) 21, 299
BstNI CCWGG 1 cut(s) 272
BstNSI RCATGY 1 cut(s) 392
BstSCI CCNGG 2 cut(s) 173, 270
BstV1I GCAGC 5 cut(s) 166, 173, 176, 202, 280
BsuRI GGCC 2 cut(s) 178, 280
BtsCI GGATG 1 cut(s) 348
BtsIMutI CAGTG 1 cut(s) 156
Cac8I GCNNGC 1 cut(s) 159
CaiI CAGNNNCTG 1 cut(s) 296
Cfr13I GGNCC 1 cut(s) 176
CseI GACGC 1 cut(s) 245
Csp6I GTAC 3 cut(s) 204, 210, 486
CviAII CATG 2 cut(s) 356, 389
CviJI RGCY 8 cut(s) 79, 157, 167, 178, 270, 280, 293, 437
CviKI_1 RGCY 8 cut(s) 79, 157, 167, 178, 270, 280, 293, 437
CviQI GTAC 3 cut(s) 204, 210, 486
DdeI CTNAG 1 cut(s) 252
DpnI GATC 3 cut(s) 148, 312, 402
DpnII GATC 3 cut(s) 146, 310, 400
Eco57I CTGAAG 1 cut(s) 459
EcoRII CCWGG 1 cut(s) 270
FaeI CATG 2 cut(s) 359, 392
FaiI YATR 3 cut(s) 357, 366, 390
FatI CATG 2 cut(s) 355, 388
FauI CCCGC 1 cut(s) 390
FblI GTMKAC 1 cut(s) 128
Fnu4HI GCNGC 5 cut(s) 155, 162, 165, 216, 294
FokI GGATG 1 cut(s) 335
Fsp4HI GCNGC 5 cut(s) 155, 162, 165, 216, 294
FspBI CTAG 2 cut(s) 207, 236
GluI GCNGC 5 cut(s) 155, 162, 165, 216, 294
HaeIII GGCC 2 cut(s) 178, 280
HapII CCGG 2 cut(s) 174, 416
HgaI GACGC 1 cut(s) 245
Hin1I GRCGYC 1 cut(s) 125
Hin1II CATG 2 cut(s) 359, 392
HincII GTYRAC 1 cut(s) 129
HindII GTYRAC 1 cut(s) 129
HindIII AAGCTT 1 cut(s) 77
HpaII CCGG 2 cut(s) 174, 416
Hpy166II GTNNAC 1 cut(s) 129
Hpy188I TCNGA 2 cut(s) 146, 261
Hpy8I GTNNAC 1 cut(s) 129
Hpy99I CGWCG 4 cut(s) 124, 127, 130, 197
HpyAV CCTTC 1 cut(s) 447
HpyCH4III ACNGT 1 cut(s) 370
HpyCH4IV ACGT 2 cut(s) 125, 192
HpyCH4V TGCA 2 cut(s) 154, 386
HpyF10VI GCNNNNNNNGC 2 cut(s) 21, 299
HpyF3I CTNAG 1 cut(s) 252
HpySE526I ACGT 2 cut(s) 125, 192
Hsp92I GRCGYC 1 cut(s) 125
Hsp92II CATG 2 cut(s) 359, 392
Kzo9I GATC 3 cut(s) 146, 310, 400
LpnPI CCDG 8 cut(s) 171, 187, 192, 257, 284, 294, 303, 429
Lsp1109I GCAGC 5 cut(s) 166, 173, 176, 202, 280
MaeI CTAG 2 cut(s) 207, 236
MaeII ACGT 2 cut(s) 125, 192
MaeIII GTNAC 2 cut(s) 337, 370
MalI GATC 3 cut(s) 148, 312, 402
MbiI CCGCTC 1 cut(s) 15
MboI GATC 3 cut(s) 146, 310, 400
MboII GAAGA 1 cut(s) 452
MnlI CCTC 7 cut(s) 220, 258, 261, 277, 363, 450, 453
MseI TTAA 1 cut(s) 75
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 2 cut(s) 174, 416
MspR9I CCNGG 2 cut(s) 175, 272
MvaI CCWGG 1 cut(s) 272
MwoI GCNNNNNNNGC 2 cut(s) 21, 299
NciI CCSGG 1 cut(s) 175
NdeII GATC 3 cut(s) 146, 310, 400
NlaIII CATG 2 cut(s) 359, 392
NlaIV GGNNCC 2 cut(s) 115, 288
NmuCI GTSAC 2 cut(s) 337, 370
NspI RCATGY 1 cut(s) 392
NspV TTCGAA 1 cut(s) 451
PciI ACATGT 1 cut(s) 388
PcsI WCGNNNNNNNCGW 1 cut(s) 125
PflFI GACNNNGTC 1 cut(s) 124
PkrI GCNGC 5 cut(s) 156, 163, 166, 217, 295
PscI ACATGT 1 cut(s) 388
Psp6I CCWGG 1 cut(s) 270
PspGI CCWGG 1 cut(s) 270
PspN4I GGNNCC 2 cut(s) 115, 288
PspPI GGNCC 1 cut(s) 176
PstNI CAGNNNCTG 1 cut(s) 296
PsyI GACNNNGTC 1 cut(s) 124
PvuII CAGCTG 1 cut(s) 293
RsaI GTAC 3 cut(s) 205, 211, 487
RsaNI GTAC 3 cut(s) 204, 210, 486
SalI GTCGAC 1 cut(s) 127
SaqAI TTAA 1 cut(s) 75
SatI GCNGC 5 cut(s) 155, 162, 165, 216, 294
Sau3AI GATC 3 cut(s) 146, 310, 400
Sau96I GGNCC 1 cut(s) 176
ScaI AGTACT 2 cut(s) 211, 487
ScrFI CCNGG 2 cut(s) 175, 272
SetI ASST 8 cut(s) 81, 128, 169, 195, 231, 250, 295, 439
SfuI TTCGAA 1 cut(s) 451
SpeI ACTAGT 1 cut(s) 206
SsiI CCGC 2 cut(s) 15, 397
SspMI CTAG 2 cut(s) 207, 236
StyD4I CCNGG 2 cut(s) 173, 270
TaaI ACNGT 1 cut(s) 370
TaiI ACGT 2 cut(s) 128, 195
TaqI TCGA 5 cut(s) 65, 128, 304, 433, 451
TatI WGTACW 2 cut(s) 209, 485
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TscAI CASTG 1 cut(s) 156
TseFI GTSAC 2 cut(s) 337, 370
TseI GCWGC 5 cut(s) 154, 161, 164, 215, 293
Tsp45I GTSAC 2 cut(s) 337, 370
TspDTI ATGAA 1 cut(s) 72
TspGWI ACGGA 1 cut(s) 369
TspRI CASTG 1 cut(s) 156
Tth111I GACNNNGTC 1 cut(s) 124
XceI RCATGY 1 cut(s) 392
XmiI GTMKAC 1 cut(s) 128
XspI CTAG 2 cut(s) 207, 236
ZraI GACGTC 1 cut(s) 126
ZrmI AGTACT 2 cut(s) 211, 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.