pycom13g07250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0018432
Physical Location & Seq
Forward (+)
4791561 .. 4792133
573 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g07250.1

Sequence Viewer

Length: 573 bp
ATGAAACACAAGGGCAGTAGTACTAGTCGCAAGAAGAAATCTCTGTGCGAGAAATCGATGATTTTGGTGGCAAACATCATTAAACTTTCATCTCTCTCTCTAGGCAATATCAATCTTGGAACAACCAGGATCAAGATTCAGCAGCCACCGGAGCCAGCAGCTGCTCGTGCTAAAGTTACCGAAGTAGACGTCCCTGGTCATCACAGTTCCAGGAGCCAGCCGAAAAGATCCCAGGAACCAGAAAACGAATCAAAGCCGTTCTCCCTCGTGATGCAGGTACCAAGCACAAATCAGAATACTAGCACTACTGCTGCTGCTACTGCTGCAAGTACTAGTACTCAACCATCGTACGTGAGTGAGAAGAGTAAGCGCGACACTGGAGGCGATGTTAATCAGAGAGCTGCAGATTTTATTGAAAAGATGCGTGCCAAGATTCAGAAGAACCAAGACGATCAACTAGTAGTGAACCTCACTCCTGGTACTACTACGGCTGGAATAGTATCATCGAAACATACTTCACGTGCACTGTTTGCCCCGGCTCCTAAAATATCCAAGCGGCACTACTACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

20.59

Weight (kDa)

10.15

Isoelectric Point (pI)

53.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018432)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g35350
pyrus_communis pycom13g07250 pycom16g07220
rosa_laevigata RLG00000005782
rosa_roxburghii Rroxscaffold_5G00385460
rosa_rugosa Rorug04G0356800
rosa_samantha Rh4AG418400 Rh4BG430100 Rh4CG444900 Rh4DG426100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 192
Acc36I ACCTGC 1 cut(s) 265
Acc65I GGTACC 1 cut(s) 277
AccB1I GGYRCC 1 cut(s) 277
AccI GTMKAC 1 cut(s) 186
AccII CGCG 1 cut(s) 372
AciI CCGC 1 cut(s) 556
AclWI GGATC 2 cut(s) 137, 222
AcvI CACGTG 1 cut(s) 521
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 6 cut(s) 22, 279, 331, 337, 350, 481
AgsI TTSAA 1 cut(s) 416
AhlI ACTAGT 3 cut(s) 23, 332, 457
AjnI CCWGG 5 cut(s) 125, 193, 209, 231, 475
AluBI AGCT 2 cut(s) 161, 401
AluI AGCT 2 cut(s) 161, 401
Alw21I GWGCWC 1 cut(s) 526
Alw44I GTGCAC 1 cut(s) 522
AlwI GGATC 2 cut(s) 137, 222
AlwNI CAGNNNCTG 1 cut(s) 161
ApaLI GTGCAC 1 cut(s) 522
ApeKI GCWGC 7 cut(s) 142, 158, 161, 311, 314, 323, 401
ArsI GACNNNNNNTTYG 2 cut(s) 174, 206
Asp718I GGTACC 1 cut(s) 277
AspLEI GCGC 1 cut(s) 372
AsuC2I CCSGG 1 cut(s) 536
BaeGI GKGCMC 1 cut(s) 526
BanI GGYRCC 1 cut(s) 277
BauI CACGAG 2 cut(s) 165, 266
BbrPI CACGTG 1 cut(s) 521
Bbv12I GWGCWC 1 cut(s) 526
BbvI GCAGC 7 cut(s) 148, 154, 170, 298, 301, 310, 388
BccI CCATC 1 cut(s) 352
BceAI ACGGC 2 cut(s) 241, 504
BciT130I CCWGG 5 cut(s) 127, 195, 211, 233, 477
BcnI CCSGG 1 cut(s) 536
BcuI ACTAGT 3 cut(s) 23, 332, 457
BfaI CTAG 5 cut(s) 24, 101, 300, 333, 458
BfmI CTRYAG 1 cut(s) 402
BfuAI ACCTGC 1 cut(s) 265
BisI GCNGC 8 cut(s) 143, 159, 162, 312, 315, 324, 402, 557
BlsI GCNGC 8 cut(s) 144, 160, 163, 313, 316, 325, 403, 558
BmcAI AGTACT 3 cut(s) 22, 331, 337
Bme1390I CCNGG 6 cut(s) 127, 195, 211, 233, 477, 536
BmiI GGNNCC 5 cut(s) 153, 215, 237, 279, 540
BmrFI CCNGG 6 cut(s) 127, 195, 211, 233, 477, 536
BmsI GCATC 2 cut(s) 261, 411
BpmI CTGGAG 1 cut(s) 399
BpuMI CCSGG 1 cut(s) 536
Bsa29I ATCGAT 1 cut(s) 56
BsaAI YACGTR 2 cut(s) 352, 521
BsaBI GATNNNNATC 1 cut(s) 390
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 3 cut(s) 193, 231, 534
BsaWI WCCGGW 1 cut(s) 148
BsaXI ACNNNNNCTCC 2 cut(s) 372, 402
Bse1I ACTGG 1 cut(s) 382
Bse8I GATNNNNATC 1 cut(s) 390
BseBI CCWGG 5 cut(s) 127, 195, 211, 233, 477
BseCI ATCGAT 1 cut(s) 56
BseDI CCNNGG 3 cut(s) 193, 231, 534
BseJI GATNNNNATC 1 cut(s) 390
BseNI ACTGG 1 cut(s) 382
BseSI GKGCMC 1 cut(s) 526
BseXI GCAGC 7 cut(s) 148, 154, 170, 298, 301, 310, 388
Bsh1236I CGCG 1 cut(s) 372
BshNI GGYRCC 1 cut(s) 277
BshVI ATCGAT 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 526
BsiSI CCGG 2 cut(s) 149, 536
BsiWI CGTACG 1 cut(s) 348
BslFI GGGAC 1 cut(s) 176
BsmFI GGGAC 1 cut(s) 176
Bsp1286I GDGCHC 1 cut(s) 526
Bsp143I GATC 3 cut(s) 129, 227, 451
BspACI CCGC 1 cut(s) 556
BspDI ATCGAT 1 cut(s) 56
BspFNI CGCG 1 cut(s) 372
BspLI GGNNCC 5 cut(s) 153, 215, 237, 279, 540
BspMAI CTGCAG 1 cut(s) 406
BspMI ACCTGC 1 cut(s) 265
BspPI GGATC 2 cut(s) 137, 222
BspT107I GGYRCC 1 cut(s) 277
BsrI ACTGG 1 cut(s) 382
BssECI CCNNGG 3 cut(s) 193, 231, 534
BssMI GATC 3 cut(s) 129, 227, 451
BssNI GRCGYC 1 cut(s) 189
BssSI CACGAG 2 cut(s) 165, 266
Bst2BI CACGAG 2 cut(s) 165, 266
Bst2UI CCWGG 5 cut(s) 127, 195, 211, 233, 477
Bst4CI ACNGT 2 cut(s) 206, 528
Bst6I CTCTTC 1 cut(s) 356
BstACI GRCGYC 1 cut(s) 189
BstAPI GCANNNNNTGC 1 cut(s) 530
BstBAI YACGTR 2 cut(s) 352, 521
BstC8I GCNNGC 3 cut(s) 156, 218, 426
BstFNI CGCG 1 cut(s) 372
BstHHI GCGC 1 cut(s) 372
BstKTI GATC 3 cut(s) 132, 230, 454
BstMBI GATC 3 cut(s) 129, 227, 451
BstMWI GCNNNNNNNGC 5 cut(s) 151, 167, 320, 323, 530
BstNI CCWGG 5 cut(s) 127, 195, 211, 233, 477
BstSCI CCNGG 6 cut(s) 125, 193, 209, 231, 475, 534
BstSFI CTRYAG 1 cut(s) 402
BstSLI GKGCMC 1 cut(s) 526
BstUI CGCG 1 cut(s) 372
BstV1I GCAGC 7 cut(s) 148, 154, 170, 298, 301, 310, 388
BstX2I RGATCY 1 cut(s) 227
BstYI RGATCY 1 cut(s) 227
Bsu15I ATCGAT 1 cut(s) 56
BsuTUI ATCGAT 1 cut(s) 56
BtgZI GCGATG 1 cut(s) 399
BtsIMutI CAGTG 2 cut(s) 375, 524
BveI ACCTGC 1 cut(s) 265
Cac8I GCNNGC 3 cut(s) 156, 218, 426
CaiI CAGNNNCTG 1 cut(s) 161
CfoI GCGC 1 cut(s) 372
ClaI ATCGAT 1 cut(s) 56
Csp6I GTAC 6 cut(s) 21, 278, 330, 336, 349, 480
CviJI RGCY 9 cut(s) 145, 154, 161, 216, 220, 256, 401, 491, 539
CviKI_1 RGCY 9 cut(s) 145, 154, 161, 216, 220, 256, 401, 491, 539
CviQI GTAC 6 cut(s) 21, 278, 330, 336, 349, 480
DpnI GATC 3 cut(s) 131, 229, 453
DpnII GATC 3 cut(s) 129, 227, 451
Eam1104I CTCTTC 1 cut(s) 356
EarI CTCTTC 1 cut(s) 356
Eco72I CACGTG 1 cut(s) 521
EcoRII CCWGG 5 cut(s) 125, 193, 209, 231, 475
FaiI YATR 1 cut(s) 513
FaqI GGGAC 1 cut(s) 176
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 8 cut(s) 143, 159, 162, 312, 315, 324, 402, 557
Fsp4HI GCNGC 8 cut(s) 143, 159, 162, 312, 315, 324, 402, 557
FspBI CTAG 5 cut(s) 24, 101, 300, 333, 458
GlaI GCGC 1 cut(s) 371
GluI GCNGC 8 cut(s) 143, 159, 162, 312, 315, 324, 402, 557
GsuI CTGGAG 1 cut(s) 399
HapII CCGG 2 cut(s) 149, 536
HhaI GCGC 1 cut(s) 372
Hin1I GRCGYC 1 cut(s) 189
Hin6I GCGC 1 cut(s) 370
HinP1I GCGC 1 cut(s) 370
HinfI GANTC 3 cut(s) 136, 248, 433
HpaII CCGG 2 cut(s) 149, 536
Hpy166II GTNNAC 3 cut(s) 187, 466, 524
Hpy188I TCNGA 3 cut(s) 294, 396, 438
Hpy188III TCNNGA 2 cut(s) 133, 268
Hpy8I GTNNAC 3 cut(s) 187, 466, 524
HpyCH4III ACNGT 2 cut(s) 206, 528
HpyCH4IV ACGT 3 cut(s) 189, 351, 520
HpyCH4V TGCA 4 cut(s) 274, 326, 404, 524
HpyF10VI GCNNNNNNNGC 5 cut(s) 151, 167, 320, 323, 530
HpySE526I ACGT 3 cut(s) 189, 351, 520
Hsp92I GRCGYC 1 cut(s) 189
HspAI GCGC 1 cut(s) 370
KpnI GGTACC 1 cut(s) 281
Kzo9I GATC 3 cut(s) 129, 227, 451
LmnI GCTCC 3 cut(s) 151, 213, 544
Lsp1109I GCAGC 7 cut(s) 148, 154, 170, 298, 301, 310, 388
LweI GCATC 2 cut(s) 261, 411
MaeI CTAG 5 cut(s) 24, 101, 300, 333, 458
MaeII ACGT 3 cut(s) 189, 351, 520
MaeIII GTNAC 1 cut(s) 175
MalI GATC 3 cut(s) 131, 229, 453
MboI GATC 3 cut(s) 129, 227, 451
MboII GAAGA 3 cut(s) 46, 373, 451
MflI RGATCY 1 cut(s) 227
MhlI GDGCHC 1 cut(s) 526
MnlI CCTC 3 cut(s) 275, 374, 479
MseI TTAA 3 cut(s) 81, 390, 571
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 2 cut(s) 149, 536
MspR9I CCNGG 6 cut(s) 127, 195, 211, 233, 477, 536
MvaI CCWGG 5 cut(s) 127, 195, 211, 233, 477
MvnI CGCG 1 cut(s) 372
MwoI GCNNNNNNNGC 5 cut(s) 151, 167, 320, 323, 530
NciI CCSGG 1 cut(s) 536
NdeII GATC 3 cut(s) 129, 227, 451
NlaIV GGNNCC 5 cut(s) 153, 215, 237, 279, 540
PfeI GAWTC 3 cut(s) 136, 248, 433
Pfl23II CGTACG 1 cut(s) 348
PfoI TCCNGGA 1 cut(s) 209
PkrI GCNGC 8 cut(s) 144, 160, 163, 313, 316, 325, 403, 558
PmaCI CACGTG 1 cut(s) 521
PmlI CACGTG 1 cut(s) 521
Ppu21I YACGTR 2 cut(s) 352, 521
Psp6I CCWGG 5 cut(s) 125, 193, 209, 231, 475
PspCI CACGTG 1 cut(s) 521
PspGI CCWGG 5 cut(s) 125, 193, 209, 231, 475
PspLI CGTACG 1 cut(s) 348
PspN4I GGNNCC 5 cut(s) 153, 215, 237, 279, 540
PstI CTGCAG 1 cut(s) 406
PstNI CAGNNNCTG 1 cut(s) 161
PsuI RGATCY 1 cut(s) 227
PvuII CAGCTG 1 cut(s) 161
RsaI GTAC 6 cut(s) 22, 279, 331, 337, 350, 481
RsaNI GTAC 6 cut(s) 21, 278, 330, 336, 349, 480
SaqAI TTAA 3 cut(s) 81, 390, 571
SatI GCNGC 8 cut(s) 143, 159, 162, 312, 315, 324, 402, 557
Sau3AI GATC 3 cut(s) 129, 227, 451
ScaI AGTACT 3 cut(s) 22, 331, 337
ScrFI CCNGG 6 cut(s) 127, 195, 211, 233, 477, 536
SduI GDGCHC 1 cut(s) 526
SetI ASST 7 cut(s) 163, 192, 279, 354, 403, 471, 523
SfaNI GCATC 2 cut(s) 261, 411
SfcI CTRYAG 1 cut(s) 402
SpeI ACTAGT 3 cut(s) 23, 332, 457
SsiI CCGC 1 cut(s) 556
SspMI CTAG 5 cut(s) 24, 101, 300, 333, 458
StyD4I CCNGG 6 cut(s) 125, 193, 209, 231, 475, 534
TaaI ACNGT 2 cut(s) 206, 528
TaiI ACGT 3 cut(s) 192, 354, 523
TaqI TCGA 2 cut(s) 56, 506
TatI WGTACW 3 cut(s) 20, 329, 335
TauI GCSGC 1 cut(s) 559
TfiI GAWTC 3 cut(s) 136, 248, 433
Tru1I TTAA 3 cut(s) 81, 390, 571
Tru9I TTAA 3 cut(s) 81, 390, 571
TscAI CASTG 2 cut(s) 382, 531
TseI GCWGC 7 cut(s) 142, 158, 161, 311, 314, 323, 401
TspDTI ATGAA 2 cut(s) 17, 78
TspRI CASTG 2 cut(s) 382, 531
VneI GTGCAC 1 cut(s) 522
XmiI GTMKAC 1 cut(s) 186
XspI CTAG 5 cut(s) 24, 101, 300, 333, 458
ZraI GACGTC 1 cut(s) 190
ZrmI AGTACT 3 cut(s) 22, 331, 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.