FvH4_4g36511

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0019703
Physical Location & Seq
Reverse (-)
33420745 .. 33422776
2032 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g36511.t1

Sequence Viewer

Length: 1230 bp
ATGGGAGGTGCGCAGAATAAGTACCATGATTCATTAGGCAAGATATTTCCGGAAATATTAGAGGCTCAATTCCAAGTGGCTTACAAATCCGGCAACAATGTTTCGAAACTGCTAAAAACCGAAGATGTTGGAGCGCAAAGTTGTGGTGAAGGAACGTTCAGTAAGCTATGCCGGTCACTCACGACTAGTCTCTCCGTCAGAGTTGGTTCGGCTTGTAATGTTGAATTGGGGGATAAATTGCCCAGGAATAAGCAGCATTCTTCTACACTGAAGGAGTTATGCGAATGGGAGACAAAGCTCTATAATGAGGTGAAGAAACTCGACAAGGATGGAGGTGAGGTTGCCAAAAGCTGTGCGGTATACAATTTCAAAGAAATCCAAAAACTGAGAGATGGAGAGCTGCAGCAGCAAATCGGTGAGCTGCTAAAAGCCCTATGGAGAGATTGGAAGGTGATGTCAGAATGCCATAGCATCCAAAAGGACATCATGTCCAAAGCAGAAACATATGATGTATTAAAGATCAACGATGCCTCAACCTATAAGAAAATTAAAGTGCTTAAACAAGACCTTTCCAATTGGAGAAGATGCTTTGCTGCATATATTTCTTCACAAAAGGCCTACGTTGGAGCTCTTGACAGTTGGGTCAGCCACTCGTTCCCATCGTTTTGTGAAGATGACATTCATGAAAACCACATTCTATCTTTGCTCTTGAAAACGTGGTCATCAGCTGGTTTGGAGGAGCTGCCTGTCAAGGAAGTAACTGATGCCATGAAAAGCTTGGAGAAACAAGTTGAAGAACTGAAGGTTCAACTGAGGAAGGAAGGGAAACAAAAGAGGAAGGTTAAGAGACTCGAGAGAAGGGTTCAGGAACTTAAGATAAATGTTATTAAATTTGATGATGAGGGGGACGATGAAAAGTTGGAGAGTTTGGAGTACAAGCTAAGCGAAGAGGAAAAGGCGCTCCATAGCAGGATAGAGAAGCGAGAGGTCGCAGCAAATGCTGTTTGTACTGGATTTTCATCAGTCTTTGAATCCTTGGCTGAGTTTTCAGAGACTTGTTATGAAAAAATCGCTGAGATTTCAGTGATCGACAAAAGGGAACACAAGATCCAGAGAGCGTATTCAGCGCACCTGTCCATCTCCGCCGTCTATTTGACGCGGACTTTTGACTTTTTCAAAATGGACGGCGGAGATAGACGGGTGCGCGGCGCACCCGGGTGCGCTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

410

Amino Acids

46.52

Weight (kDa)

7.89

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF632 PF04782 20 - 118 3.6e-11 Protein of unknown function (DUF632)
DUF632 PF04782 124 - 265 4.8e-30 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019703)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g36511
rosa_multiflora Rmu_sc0005271.1_g000001
rosa_roxburghii Rroxscaffold_5G00357090 Rroxscaffold_5G00386680
rosa_samantha Rh4BG440700 Rh4CG458000 Rh4DG439200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 641
Acc16I TGCGCA 1 cut(s) 12
AccI GTMKAC 1 cut(s) 360
AccII CGCG 2 cut(s) 1159, 1206
AccIII TCCGGA 1 cut(s) 49
AciI CCGC 5 cut(s) 356, 1143, 1159, 1188, 1206
AclI AACGTT 1 cut(s) 155
AclWI GGATC 1 cut(s) 1102
AcsI RAATTY 1 cut(s) 890
AcuI CTGAAG 2 cut(s) 290, 821
AfaI GTAC 3 cut(s) 23, 935, 1009
AflII CTTAAG 1 cut(s) 872
AgsI TTSAA 7 cut(s) 224, 370, 712, 794, 809, 1031, 1177
AhdI GACNNNNNGTC 1 cut(s) 487
AhlI ACTAGT 1 cut(s) 185
AjnI CCWGG 1 cut(s) 242
AleI CACNNNNGTG 1 cut(s) 1216
AloI GAACNNNNNNTCC 2 cut(s) 1092, 1124
Alw21I GWGCWC 1 cut(s) 631
Alw26I GTCTC 4 cut(s) 194, 284, 841, 1046
AlwI GGATC 1 cut(s) 1102
Ama87I CYCGRG 2 cut(s) 851, 1214
Aor13HI TCCGGA 1 cut(s) 49
AoxI GGCC 1 cut(s) 615
ApeKI GCWGC 8 cut(s) 253, 400, 403, 406, 421, 593, 742, 992
ApoI RAATTY 1 cut(s) 890
AspLEI GCGC 7 cut(s) 13, 136, 961, 1129, 1206, 1211, 1223
AsuC2I CCSGG 2 cut(s) 1215, 1216
AsuHPI GGTGA 5 cut(s) 158, 322, 347, 428, 463
AsuII TTCGAA 1 cut(s) 104
AvaI CYCGRG 2 cut(s) 851, 1214
BanII GRGCYC 1 cut(s) 631
Bbv12I GWGCWC 1 cut(s) 631
BbvI GCAGC 8 cut(s) 265, 387, 408, 415, 418, 580, 729, 1004
BccI CCATC 4 cut(s) 323, 386, 667, 1145
BceAI ACGGC 2 cut(s) 1130, 1201
BciT130I CCWGG 1 cut(s) 244
BcnI CCSGG 2 cut(s) 1215, 1216
BcoDI GTCTC 4 cut(s) 194, 284, 841, 1046
BcuI ACTAGT 1 cut(s) 185
BfaI CTAG 1 cut(s) 186
BfmI CTRYAG 1 cut(s) 401
BfoI RGCGCY 1 cut(s) 962
BfrI CTTAAG 1 cut(s) 872
BisI GCNGC 9 cut(s) 254, 401, 404, 407, 422, 594, 743, 993, 1207
BlpI GCTNAGC 1 cut(s) 941
BlsI GCNGC 9 cut(s) 255, 402, 405, 408, 423, 595, 744, 994, 1208
Bme1390I CCNGG 3 cut(s) 244, 1215, 1216
BmeRI GACNNNNNGTC 1 cut(s) 487
BmeT110I CYCGRG 2 cut(s) 851, 1214
BmrFI CCNGG 3 cut(s) 244, 1215, 1216
BmsI GCATC 4 cut(s) 480, 517, 575, 754
Bpu1102I GCTNAGC 1 cut(s) 941
Bpu14I TTCGAA 1 cut(s) 104
BpuMI CCSGG 2 cut(s) 1215, 1216
BsaBI GATNNNNATC 1 cut(s) 1018
BsaJI CCNNGG 3 cut(s) 242, 1035, 1214
BsaWI WCCGGW 1 cut(s) 49
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bse118I RCCGGY 1 cut(s) 171
Bse1I ACTGG 1 cut(s) 1015
Bse8I GATNNNNATC 1 cut(s) 1018
BseAI TCCGGA 1 cut(s) 49
BseBI CCWGG 1 cut(s) 244
BseDI CCNNGG 3 cut(s) 242, 1035, 1214
BseGI GGATG 2 cut(s) 334, 471
BseJI GATNNNNATC 1 cut(s) 1018
BseMII CTCAG 4 cut(s) 377, 803, 1032, 1065
BseNI ACTGG 1 cut(s) 1015
BseRI GAGGAG 1 cut(s) 752
BseXI GCAGC 8 cut(s) 265, 387, 408, 415, 418, 580, 729, 1004
Bsh1236I CGCG 2 cut(s) 1159, 1206
BshFI GGCC 1 cut(s) 617
BsiHKAI GWGCWC 1 cut(s) 631
BsiHKCI CYCGRG 2 cut(s) 851, 1214
BsiSI CCGG 4 cut(s) 50, 90, 172, 1215
BslFI GGGAC 1 cut(s) 920
BsmAI GTCTC 4 cut(s) 194, 284, 841, 1046
BsmFI GGGAC 1 cut(s) 920
BsmI GAATGC 2 cut(s) 256, 467
BsnI GGCC 1 cut(s) 617
BsoBI CYCGRG 2 cut(s) 851, 1214
Bsp119I TTCGAA 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 631
Bsp13I TCCGGA 1 cut(s) 49
Bsp143I GATC 3 cut(s) 519, 1086, 1107
Bsp1720I GCTNAGC 1 cut(s) 941
BspACI CCGC 5 cut(s) 356, 1143, 1159, 1188, 1206
BspANI GGCC 1 cut(s) 617
BspCNI CTCAG 4 cut(s) 378, 804, 1033, 1066
BspEI TCCGGA 1 cut(s) 49
BspFNI CGCG 2 cut(s) 1159, 1206
BspHI TCATGA 1 cut(s) 682
BspMAI CTGCAG 1 cut(s) 405
BspPI GGATC 1 cut(s) 1102
BspT104I TTCGAA 1 cut(s) 104
BspTI CTTAAG 1 cut(s) 872
BsrFI RCCGGY 1 cut(s) 171
BsrI ACTGG 1 cut(s) 1015
BssAI RCCGGY 1 cut(s) 171
BssECI CCNNGG 3 cut(s) 242, 1035, 1214
BssMI GATC 3 cut(s) 519, 1086, 1107
BssNAI GTATAC 1 cut(s) 361
BssT1I CCWWGG 1 cut(s) 1035
Bst1107I GTATAC 1 cut(s) 361
Bst2UI CCWGG 1 cut(s) 244
Bst4CI ACNGT 1 cut(s) 638
Bst6I CTCTTC 1 cut(s) 942
BstAFI CTTAAG 1 cut(s) 872
BstAPI GCANNNNNTGC 1 cut(s) 998
BstBI TTCGAA 1 cut(s) 104
BstDEI CTNAG 5 cut(s) 386, 812, 941, 1041, 1074
BstF5I GGATG 2 cut(s) 334, 471
BstFNI CGCG 2 cut(s) 1159, 1206
BstH2I RGCGCY 1 cut(s) 962
BstHHI GCGC 7 cut(s) 13, 136, 961, 1129, 1206, 1211, 1223
BstKTI GATC 3 cut(s) 522, 1089, 1110
BstMAI GTCTC 4 cut(s) 194, 284, 841, 1046
BstMBI GATC 3 cut(s) 519, 1086, 1107
BstMWI GCNNNNNNNGC 3 cut(s) 406, 998, 1124
BstNI CCWGG 1 cut(s) 244
BstSCI CCNGG 3 cut(s) 242, 1213, 1214
BstSFI CTRYAG 1 cut(s) 401
BstUI CGCG 2 cut(s) 1159, 1206
BstV1I GCAGC 8 cut(s) 265, 387, 408, 415, 418, 580, 729, 1004
BstX2I RGATCY 1 cut(s) 1107
BstYI RGATCY 1 cut(s) 1107
BstZ17I GTATAC 1 cut(s) 361
BsuRI GGCC 1 cut(s) 617
BtsCI GGATG 2 cut(s) 334, 471
BtsIMutI CAGTG 2 cut(s) 266, 1089
CciI TCATGA 1 cut(s) 682
CfoI GCGC 7 cut(s) 13, 136, 961, 1129, 1206, 1211, 1223
Cfr10I RCCGGY 1 cut(s) 171
Cfr9I CCCGGG 1 cut(s) 1214
CseI GACGC 1 cut(s) 1165
Csp6I GTAC 3 cut(s) 22, 934, 1008
CviAII CATG 4 cut(s) 26, 487, 683, 769
CviQI GTAC 3 cut(s) 22, 934, 1008
DdeI CTNAG 5 cut(s) 386, 812, 941, 1041, 1074
DpnI GATC 3 cut(s) 521, 1088, 1109
DpnII GATC 3 cut(s) 519, 1086, 1107
DrdI GACNNNNNNGTC 1 cut(s) 641
DriI GACNNNNNGTC 1 cut(s) 487
DseDI GACNNNNNNGTC 1 cut(s) 641
Eam1104I CTCTTC 1 cut(s) 942
Eam1105I GACNNNNNGTC 1 cut(s) 487
EarI CTCTTC 1 cut(s) 942
EciI GGCGGA 2 cut(s) 1132, 1203
Ecl136II GAGCTC 1 cut(s) 629
Eco130I CCWWGG 1 cut(s) 1035
Eco147I AGGCCT 1 cut(s) 617
Eco24I GRGCYC 1 cut(s) 631
Eco53kI GAGCTC 1 cut(s) 629
Eco57I CTGAAG 2 cut(s) 290, 821
Eco88I CYCGRG 2 cut(s) 851, 1214
EcoICRI GAGCTC 1 cut(s) 629
EcoRII CCWGG 1 cut(s) 242
EcoT14I CCWWGG 1 cut(s) 1035
EcoT38I GRGCYC 1 cut(s) 631
ErhI CCWWGG 1 cut(s) 1035
FaeI CATG 4 cut(s) 29, 490, 686, 772
FaqI GGGAC 1 cut(s) 920
FatI CATG 4 cut(s) 25, 486, 682, 768
FauNDI CATATG 1 cut(s) 505
FblI GTMKAC 1 cut(s) 360
Fnu4HI GCNGC 9 cut(s) 254, 401, 404, 407, 422, 594, 743, 993, 1207
FokI GGATG 2 cut(s) 341, 458
FriOI GRGCYC 1 cut(s) 631
Fsp4HI GCNGC 9 cut(s) 254, 401, 404, 407, 422, 594, 743, 993, 1207
FspBI CTAG 1 cut(s) 186
FspI TGCGCA 1 cut(s) 12
GlaI GCGC 7 cut(s) 12, 135, 960, 1128, 1205, 1210, 1222
GluI GCNGC 9 cut(s) 254, 401, 404, 407, 422, 594, 743, 993, 1207
HaeII RGCGCY 1 cut(s) 962
HaeIII GGCC 1 cut(s) 617
HapII CCGG 4 cut(s) 50, 90, 172, 1215
HgaI GACGC 1 cut(s) 1165
HhaI GCGC 7 cut(s) 13, 136, 961, 1129, 1206, 1211, 1223
Hin1II CATG 4 cut(s) 29, 490, 686, 772
Hin6I GCGC 7 cut(s) 11, 134, 959, 1127, 1204, 1209, 1221
HinP1I GCGC 7 cut(s) 11, 134, 959, 1127, 1204, 1209, 1221
HindIII AAGCTT 1 cut(s) 775
HinfI GANTC 3 cut(s) 29, 849, 1031
HpaII CCGG 4 cut(s) 50, 90, 172, 1215
HphI GGTGA 5 cut(s) 158, 322, 347, 428, 463
Hpy166II GTNNAC 1 cut(s) 361
Hpy188I TCNGA 3 cut(s) 200, 460, 1051
Hpy188III TCNNGA 8 cut(s) 50, 181, 632, 683, 709, 853, 866, 1111
Hpy8I GTNNAC 1 cut(s) 361
HpyAV CCTTC 8 cut(s) 143, 265, 442, 796, 811, 815, 832, 852
HpyCH4III ACNGT 1 cut(s) 638
HpyCH4IV ACGT 3 cut(s) 155, 621, 716
HpyCH4V TGCA 2 cut(s) 403, 596
HpyF10VI GCNNNNNNNGC 3 cut(s) 406, 998, 1124
HpyF3I CTNAG 5 cut(s) 386, 812, 941, 1041, 1074
HpySE526I ACGT 3 cut(s) 155, 621, 716
Hsp92II CATG 4 cut(s) 29, 490, 686, 772
HspAI GCGC 7 cut(s) 11, 134, 959, 1127, 1204, 1209, 1221
Kpn2I TCCGGA 1 cut(s) 49
Kzo9I GATC 3 cut(s) 519, 1086, 1107
LmnI GCTCC 4 cut(s) 131, 626, 739, 966
Lsp1109I GCAGC 8 cut(s) 265, 387, 408, 415, 418, 580, 729, 1004
LweI GCATC 4 cut(s) 480, 517, 575, 754
MaeI CTAG 1 cut(s) 186
MaeII ACGT 3 cut(s) 155, 621, 716
MaeIII GTNAC 2 cut(s) 174, 757
MalI GATC 3 cut(s) 521, 1088, 1109
MboI GATC 3 cut(s) 519, 1086, 1107
MboII GAAGA 8 cut(s) 134, 252, 325, 594, 597, 683, 806, 959
MfeI CAATTG 1 cut(s) 574
MflI RGATCY 1 cut(s) 1107
MhlI GDGCHC 1 cut(s) 631
MluCI AATT 7 cut(s) 68, 224, 236, 364, 546, 574, 890
MlyI GAGTC 1 cut(s) 843
MmeI TCCRAC 3 cut(s) 109, 604, 900
MroI TCCGGA 1 cut(s) 49
MseI TTAA 6 cut(s) 515, 549, 558, 843, 873, 888
MslI CAYNNNNRTG 1 cut(s) 1216
MspA1I CMGCKG 1 cut(s) 728
MspCI CTTAAG 1 cut(s) 872
MspI CCGG 4 cut(s) 50, 90, 172, 1215
MspR9I CCNGG 3 cut(s) 244, 1215, 1216
MteI GCGCNGCGC 1 cut(s) 1207
MunI CAATTG 1 cut(s) 574
Mva1269I GAATGC 2 cut(s) 256, 467
MvaI CCWGG 1 cut(s) 244
MvnI CGCG 2 cut(s) 1159, 1206
MwoI GCNNNNNNNGC 3 cut(s) 406, 998, 1124
NciI CCSGG 2 cut(s) 1215, 1216
NdeI CATATG 1 cut(s) 505
NdeII GATC 3 cut(s) 519, 1086, 1107
NlaIII CATG 4 cut(s) 29, 490, 686, 772
NmuCI GTSAC 1 cut(s) 174
NsbI TGCGCA 1 cut(s) 12
NspV TTCGAA 1 cut(s) 104
OliI CACNNNNGTG 1 cut(s) 1216
PaeR7I CTCGAG 1 cut(s) 851
PagI TCATGA 1 cut(s) 682
PceI AGGCCT 1 cut(s) 617
PcsI WCGNNNNNNNCGW 1 cut(s) 659
PctI GAATGC 2 cut(s) 256, 467
PfeI GAWTC 2 cut(s) 29, 1031
PkrI GCNGC 9 cut(s) 255, 402, 405, 408, 423, 595, 744, 994, 1208
PleI GAGTC 1 cut(s) 843
PpsI GAGTC 1 cut(s) 843
Psp124BI GAGCTC 1 cut(s) 631
Psp1406I AACGTT 1 cut(s) 155
Psp6I CCWGG 1 cut(s) 242
PspGI CCWGG 1 cut(s) 242
PstI CTGCAG 1 cut(s) 405
PsuI RGATCY 1 cut(s) 1107
PvuII CAGCTG 1 cut(s) 728
RsaI GTAC 3 cut(s) 23, 935, 1009
RsaNI GTAC 3 cut(s) 22, 934, 1008
RseI CAYNNNNRTG 1 cut(s) 1216
SacI GAGCTC 1 cut(s) 631
SaqAI TTAA 6 cut(s) 515, 549, 558, 843, 873, 888
SatI GCNGC 9 cut(s) 254, 401, 404, 407, 422, 594, 743, 993, 1207
Sau3AI GATC 3 cut(s) 519, 1086, 1107
SchI GAGTC 1 cut(s) 843
ScrFI CCNGG 3 cut(s) 244, 1215, 1216
SduI GDGCHC 1 cut(s) 631
SfaNI GCATC 4 cut(s) 480, 517, 575, 754
SfcI CTRYAG 1 cut(s) 401
Sfr274I CTCGAG 1 cut(s) 851
SfuI TTCGAA 1 cut(s) 104
SlaI CTCGAG 1 cut(s) 851
SmaI CCCGGG 1 cut(s) 1216
SmiMI CAYNNNNRTG 1 cut(s) 1216
SmlI CTYRAG 2 cut(s) 851, 872
SmoI CTYRAG 2 cut(s) 851, 872
SpeI ACTAGT 1 cut(s) 185
Sse9I AATT 7 cut(s) 68, 224, 236, 364, 546, 574, 890
SseBI AGGCCT 1 cut(s) 617
SsiI CCGC 5 cut(s) 356, 1143, 1159, 1188, 1206
SspI AATATT 1 cut(s) 57
SspMI CTAG 1 cut(s) 186
SstI GAGCTC 1 cut(s) 631
StuI AGGCCT 1 cut(s) 617
StyD4I CCNGG 3 cut(s) 242, 1213, 1214
StyI CCWWGG 1 cut(s) 1035
TaaI ACNGT 1 cut(s) 638
TaiI ACGT 3 cut(s) 158, 624, 719
TaqI TCGA 4 cut(s) 104, 321, 852, 1089
TasI AATT 7 cut(s) 68, 224, 236, 364, 546, 574, 890
TatI WGTACW 2 cut(s) 933, 1007
TauI GCSGC 1 cut(s) 1209
TfiI GAWTC 2 cut(s) 29, 1031
Tru1I TTAA 6 cut(s) 515, 549, 558, 843, 873, 888
Tru9I TTAA 6 cut(s) 515, 549, 558, 843, 873, 888
TscAI CASTG 2 cut(s) 273, 1089
TseFI GTSAC 1 cut(s) 174
TseI GCWGC 8 cut(s) 253, 400, 403, 406, 421, 593, 742, 992
Tsp45I GTSAC 1 cut(s) 174
TspDTI ATGAA 7 cut(s) 21, 671, 699, 785, 927, 1008, 1077
TspGWI ACGGA 1 cut(s) 184
TspMI CCCGGG 1 cut(s) 1214
TspRI CASTG 2 cut(s) 273, 1089
Vha464I CTTAAG 1 cut(s) 872
XapI RAATTY 1 cut(s) 890
XcmI CCANNNNNNNNNTGG 1 cut(s) 775
XhoI CTCGAG 1 cut(s) 851
XmaI CCCGGG 1 cut(s) 1214
XmiI GTMKAC 1 cut(s) 360
XspI CTAG 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.