Rh4CG458000

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0019703
Physical Location & Seq
Reverse (-)
68955933 .. 68956841
909 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG458000.1

Sequence Viewer

Length: 909 bp
ATGGGAGGTGTTAGCTCAAAATCTGGAAAAGTTGTTTGTAAAGAGAGAAAAGAGCTGTTGAAATTGGCTCTACGACGTAGGGATCAGCTTGAAGAAGCGCAAAATAAGTACCATAACTTGCATAAGAAGTTAGCAGATACCATAACAGAGTTTGTGCAAACGTATTCCTCTCACTATAAGAATTCTGAGATGAAAGTTAAGGCTCAATTCCAAAAGGCTCACGAGTCTGGCAACAATGTTTCAAAACTGCTAAAGGCCGAAGATGTTCGAAGGCAAAGTACTGCTGAAAGAAATTTTTTTAGGCTATGCCGGTCACTCACGATGAGTCGCTCCTGCATAGTTCGTTCTTCGGCTTGTGATGATGAGGATGAGGAGAAACCCAGGAAGCATACTTCTACACTGAAGAAATTATACGATTGGGAGACACAGCTCTATCATGAGATAAAGAAACTCGAGAATAATGGAGGAGGAAAGGTTGCCAAAAAATGTGTGGTATCAACTATCAAACAAGTTCAAAAACTGAGAGATGAAGAGCTGCAGCAGAAAATAGTTGAGCTGCTAAATGCCATAATGAGAGATTGGAAGGTGATGTCAGGATGTCATAGAATCCTAAAGGACATCATGTCCAAAGCAGAAAAATCTTCCTATTCTCCTCCTCCTTATGGATTAATGGTCAGCGATGCCTCACACCTGAAGAAAACCTCAGAGCTTAAACATCACATTTCCAATTGGAGAACCGGCTTTGCTTCATATATTTCTTCGCAAAAGCCTACGTTAAAGCTCTTGATGATTGGATCAGCCACTCGTTCCCATCGTTTTGTGAAGATGACAGTGACAACCACAACGATAGGCCATCGTTGCTTGCAAAGTGGTCAGATGGTTTGGAGGAGCTGCCTGAGAAGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

302

Amino Acids

34.83

Weight (kDa)

9.89

Isoelectric Point (pI)

56.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF632 PF04782 65 - 153 1.5e-13 Protein of unknown function (DUF632)
DUF632 PF04782 159 - 259 7.5e-21 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0019703)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g36511
rosa_multiflora Rmu_sc0005271.1_g000001
rosa_roxburghii Rroxscaffold_5G00357090 Rroxscaffold_5G00386680
rosa_samantha Rh4BG440700 Rh4CG458000 Rh4DG439200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 90, 802
AcsI RAATTY 2 cut(s) 181, 292
AcuI CTGAAG 2 cut(s) 422, 713
AfaI GTAC 2 cut(s) 110, 280
AfiI CCNNNNNNNGG 1 cut(s) 662
AgsI TTSAA 4 cut(s) 61, 92, 243, 515
AhdI GACNNNNNGTC 1 cut(s) 622
AjnI CCWGG 1 cut(s) 380
AluBI AGCT 9 cut(s) 15, 55, 88, 430, 535, 556, 709, 781, 891
AluI AGCT 9 cut(s) 15, 55, 88, 430, 535, 556, 709, 781, 891
Alw26I GTCTC 1 cut(s) 416
AlwI GGATC 2 cut(s) 90, 802
Ama87I CYCGRG 1 cut(s) 452
AoxI GGCC 2 cut(s) 255, 850
ApeKI GCWGC 4 cut(s) 535, 538, 556, 891
ApoI RAATTY 2 cut(s) 181, 292
AseI ATTAAT 1 cut(s) 668
Asp700I GAANNNNTTC 1 cut(s) 264
AspLEI GCGC 1 cut(s) 100
AsuHPI GGTGA 1 cut(s) 598
AsuII TTCGAA 1 cut(s) 268
AvaI CYCGRG 1 cut(s) 452
BarI GAAGNNNNNNTAC 4 cut(s) 262, 294, 395, 427
BauI CACGAG 1 cut(s) 221
BbvI GCAGC 4 cut(s) 522, 543, 550, 878
BccI CCATC 3 cut(s) 819, 861, 871
BciT130I CCWGG 1 cut(s) 382
BcoDI GTCTC 1 cut(s) 416
BfmI CTRYAG 1 cut(s) 536
BisI GCNGC 4 cut(s) 536, 539, 557, 892
BlsI GCNGC 4 cut(s) 537, 540, 558, 893
BmcAI AGTACT 1 cut(s) 280
Bme1390I CCNGG 1 cut(s) 382
BmeRI GACNNNNNGTC 1 cut(s) 622
BmeT110I CYCGRG 1 cut(s) 452
BmrFI CCNGG 1 cut(s) 382
BmsI GCATC 1 cut(s) 670
Bpu14I TTCGAA 1 cut(s) 268
BsaJI CCNNGG 1 cut(s) 380
BsaXI ACNNNNNCTCC 2 cut(s) 459, 489
Bsc4I CCNNNNNNNGG 1 cut(s) 662
Bse118I RCCGGY 2 cut(s) 309, 737
BseBI CCWGG 1 cut(s) 382
BseDI CCNNGG 1 cut(s) 380
BseGI GGATG 2 cut(s) 373, 602
BseLI CCNNNNNNNGG 1 cut(s) 662
BseMII CTCAG 4 cut(s) 177, 512, 717, 887
BseRI GAGGAG 5 cut(s) 386, 480, 642, 645, 901
BseXI GCAGC 4 cut(s) 522, 543, 550, 878
BshFI GGCC 2 cut(s) 257, 852
BsiHKCI CYCGRG 1 cut(s) 452
BsiSI CCGG 2 cut(s) 310, 738
BslI CCNNNNNNNGG 1 cut(s) 662
BsmAI GTCTC 1 cut(s) 416
BsnI GGCC 2 cut(s) 257, 852
BsoBI CYCGRG 1 cut(s) 452
Bsp119I TTCGAA 1 cut(s) 268
Bsp143I GATC 2 cut(s) 82, 794
BspANI GGCC 2 cut(s) 257, 852
BspCNI CTCAG 4 cut(s) 178, 513, 716, 888
BspHI TCATGA 1 cut(s) 436
BspMAI CTGCAG 1 cut(s) 540
BspPI GGATC 2 cut(s) 90, 802
BspQI GCTCTTC 1 cut(s) 525
BspT104I TTCGAA 1 cut(s) 268
BsrFI RCCGGY 2 cut(s) 309, 737
BssAI RCCGGY 2 cut(s) 309, 737
BssECI CCNNGG 1 cut(s) 380
BssMI GATC 2 cut(s) 82, 794
BssSI CACGAG 1 cut(s) 221
Bst2BI CACGAG 1 cut(s) 221
Bst2UI CCWGG 1 cut(s) 382
Bst4CI ACNGT 1 cut(s) 832
Bst6I CTCTTC 1 cut(s) 525
BstBI TTCGAA 1 cut(s) 268
BstC8I GCNNGC 1 cut(s) 863
BstDEI CTNAG 4 cut(s) 186, 521, 703, 896
BstF5I GGATG 2 cut(s) 373, 602
BstHHI GCGC 1 cut(s) 100
BstKTI GATC 2 cut(s) 85, 797
BstMAI GTCTC 1 cut(s) 416
BstMBI GATC 2 cut(s) 82, 794
BstMWI GCNNNNNNNGC 1 cut(s) 858
BstNI CCWGG 1 cut(s) 382
BstSCI CCNGG 1 cut(s) 380
BstSFI CTRYAG 1 cut(s) 536
BstV1I GCAGC 4 cut(s) 522, 543, 550, 878
BsuRI GGCC 2 cut(s) 257, 852
BtgZI GCGATG 1 cut(s) 693
BtsCI GGATG 2 cut(s) 373, 602
BtsIMutI CAGTG 2 cut(s) 398, 837
Cac8I GCNNGC 1 cut(s) 863
CciI TCATGA 1 cut(s) 436
CfoI GCGC 1 cut(s) 100
Cfr10I RCCGGY 2 cut(s) 309, 737
Csp6I GTAC 2 cut(s) 109, 279
CviAII CATG 2 cut(s) 437, 622
CviQI GTAC 2 cut(s) 109, 279
DdeI CTNAG 4 cut(s) 186, 521, 703, 896
DpnI GATC 2 cut(s) 84, 796
DpnII GATC 2 cut(s) 82, 794
DriI GACNNNNNGTC 1 cut(s) 622
Eam1104I CTCTTC 1 cut(s) 525
Eam1105I GACNNNNNGTC 1 cut(s) 622
EarI CTCTTC 1 cut(s) 525
Eco57I CTGAAG 2 cut(s) 422, 713
Eco88I CYCGRG 1 cut(s) 452
EcoRI GAATTC 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 380
FaeI CATG 2 cut(s) 440, 625
FatI CATG 2 cut(s) 436, 621
Fnu4HI GCNGC 4 cut(s) 536, 539, 557, 892
FokI GGATG 2 cut(s) 380, 609
Fsp4HI GCNGC 4 cut(s) 536, 539, 557, 892
GlaI GCGC 1 cut(s) 99
GluI GCNGC 4 cut(s) 536, 539, 557, 892
HaeIII GGCC 2 cut(s) 257, 852
HapII CCGG 2 cut(s) 310, 738
HhaI GCGC 1 cut(s) 100
Hin1II CATG 2 cut(s) 440, 625
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HinfI GANTC 3 cut(s) 224, 325, 606
HpaII CCGG 2 cut(s) 310, 738
HphI GGTGA 1 cut(s) 598
Hpy188I TCNGA 3 cut(s) 187, 706, 876
Hpy188III TCNNGA 7 cut(s) 24, 221, 319, 437, 454, 594, 784
Hpy99I CGWCG 1 cut(s) 78
HpyAV CCTTC 3 cut(s) 264, 577, 894
HpyCH4III ACNGT 1 cut(s) 832
HpyCH4IV ACGT 3 cut(s) 76, 161, 773
HpyCH4V TGCA 5 cut(s) 121, 157, 336, 538, 865
HpyF10VI GCNNNNNNNGC 1 cut(s) 858
HpyF3I CTNAG 4 cut(s) 186, 521, 703, 896
HpySE526I ACGT 3 cut(s) 76, 161, 773
Hsp92II CATG 2 cut(s) 440, 625
HspAI GCGC 1 cut(s) 98
Kzo9I GATC 2 cut(s) 82, 794
LguI GCTCTTC 1 cut(s) 525
LmnI GCTCC 2 cut(s) 335, 888
LpnPI CCDG 9 cut(s) 9, 213, 323, 346, 367, 394, 579, 704, 751
Lsp1109I GCAGC 4 cut(s) 522, 543, 550, 878
LweI GCATC 1 cut(s) 670
MaeII ACGT 3 cut(s) 76, 161, 773
MaeIII GTNAC 2 cut(s) 312, 832
MalI GATC 2 cut(s) 84, 796
MboI GATC 2 cut(s) 82, 794
MboII GAAGA 9 cut(s) 104, 272, 339, 415, 542, 633, 706, 750, 835
MfeI CAATTG 1 cut(s) 727
MluCI AATT 6 cut(s) 62, 181, 206, 292, 407, 727
MlyI GAGTC 2 cut(s) 233, 334
MroXI GAANNNNTTC 1 cut(s) 264
MseI TTAA 4 cut(s) 198, 668, 711, 776
MspI CCGG 2 cut(s) 310, 738
MspR9I CCNGG 1 cut(s) 382
MunI CAATTG 1 cut(s) 727
MvaI CCWGG 1 cut(s) 382
MwoI GCNNNNNNNGC 1 cut(s) 858
NdeII GATC 2 cut(s) 82, 794
NlaIII CATG 2 cut(s) 440, 625
NmuCI GTSAC 2 cut(s) 312, 832
NspV TTCGAA 1 cut(s) 268
PaeR7I CTCGAG 1 cut(s) 452
PagI TCATGA 1 cut(s) 436
PciSI GCTCTTC 1 cut(s) 525
PcsI WCGNNNNNNNCGW 1 cut(s) 811
PdmI GAANNNNTTC 1 cut(s) 264
PfeI GAWTC 1 cut(s) 606
PkrI GCNGC 4 cut(s) 537, 540, 558, 893
PleI GAGTC 2 cut(s) 232, 333
PpsI GAGTC 2 cut(s) 232, 333
PshBI ATTAAT 1 cut(s) 668
Psp6I CCWGG 1 cut(s) 380
PspGI CCWGG 1 cut(s) 380
PstI CTGCAG 1 cut(s) 540
RsaI GTAC 2 cut(s) 110, 280
RsaNI GTAC 2 cut(s) 109, 279
SapI GCTCTTC 1 cut(s) 525
SaqAI TTAA 4 cut(s) 198, 668, 711, 776
SatI GCNGC 4 cut(s) 536, 539, 557, 892
Sau3AI GATC 2 cut(s) 82, 794
ScaI AGTACT 1 cut(s) 280
SchI GAGTC 2 cut(s) 233, 334
ScrFI CCNGG 1 cut(s) 382
SfaNI GCATC 1 cut(s) 670
SfcI CTRYAG 1 cut(s) 536
Sfr274I CTCGAG 1 cut(s) 452
SfuI TTCGAA 1 cut(s) 268
SlaI CTCGAG 1 cut(s) 452
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
Sse9I AATT 6 cut(s) 62, 181, 206, 292, 407, 727
StyD4I CCNGG 1 cut(s) 380
TaaI ACNGT 1 cut(s) 832
TaiI ACGT 3 cut(s) 79, 164, 776
TaqI TCGA 2 cut(s) 268, 453
TasI AATT 6 cut(s) 62, 181, 206, 292, 407, 727
TatI WGTACW 1 cut(s) 278
TfiI GAWTC 1 cut(s) 606
Tru1I TTAA 4 cut(s) 198, 668, 711, 776
Tru9I TTAA 4 cut(s) 198, 668, 711, 776
TscAI CASTG 2 cut(s) 405, 837
TseFI GTSAC 2 cut(s) 312, 832
TseI GCWGC 4 cut(s) 535, 538, 556, 891
Tsp45I GTSAC 2 cut(s) 312, 832
TspDTI ATGAA 3 cut(s) 206, 543, 738
TspRI CASTG 2 cut(s) 405, 837
VspI ATTAAT 1 cut(s) 668
XapI RAATTY 2 cut(s) 181, 292
XcmI CCANNNNNNNNNTGG 1 cut(s) 487
XhoI CTCGAG 1 cut(s) 452
XmnI GAANNNNTTC 1 cut(s) 264
ZrmI AGTACT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.