FvH4_4g37320

Plant protein of unknown function (DUF639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005385
Physical Location & Seq
Reverse (-)
33835849 .. 33842936
7088 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g37320.t4

Sequence Viewer

Length: 1629 bp
ATGGAGAATTTGAAACTGGGGATGTTGGATCAGTTGGATGGTTTCTTGACGAAAAACCATCAGAAGACTCTGAAATCTCTGTTCCGGCGGAGCAGCAGCACCAAGTCCAGCGGCGAAGATGACTCCCCTTCCTCCGCCTCCGATTCTCCCAAGCCCATTCCTCACCTCTCTCCTTTCGCCAATTCCGTCGTCTCTCGCTGTTCCAAGATTCTTCGCATCCCTACTGAAGAATTACAGCATCATTTTGACACACAGCTACCTGAAAGTGTAAAGGAGCTTCTGACTTATGCTAGGAACTTTTTGGAATTTTGCTCATACCAAGCACTCCATGTAGTGAGTGGTCGCCCAGATTATTTGAGTGATAAGGAGTTTCGTCACCTGACATTTGACATGATGCTTGCATGGGAATCCCCCTGTGCTGAGAATAATAAAGAACTAAACAAAGAAACTGCTTCTAGCAGTAACCTAGAAGTAGAGGATGATGATGGTTGGTCGCTATTTTATTCAAGTTCTACGAATATGGCTGTCCAGGTTGATGACAAAAAAACTGTTGGGCCCGAAGCCTTTGCGCGAATAGCTCCTGGTTGTGCTGCTGTTGCAGATATAATAACTGTCCACAACCTCTATGATTCACTCACAAGCACATCAGGCCATCATCGACTTCATTTCCTTGTATATGACAAATACATACGAAGCCTGGACAAGGTCATTAAGGCTTCAAAAAGTACATTGGCTTCTTCCATTGGAAATCTTCAGCTCGCTGAGGGAGAAATCATCCTTGATGTTGATGGTACAGTTCCCACTCAACCAGTGCTCAAGCATATTGGCAGGTCTATGTGGCCTGGACGGCTGTCACTGACCAATAGTGCGCTGTATTTTGAATCATTGGGAGTTGGTTTGTATGACAAAGCGGTTAGATATGATCTGGCAACAGATATGAAGCAGGTCATAAAACCAGAAATGACTGGACCATTGGGTACTCGTCTTTTTGATAAAGCCATCATGTACAAATCAACGTCTGTAGAAGAGCCGGTTCATTTGGAGTTTCCTGAGTTTAAAGGCAATTCTCGCCGAGACTATTGGTTGGATATTTGTCTTGAAATCTTGCGTGCTCACAGGTTCATTCAGAAAAACAACCTAAAAGAAATTCAAAAATCAGAAGTACTGGCAAGGGCTATTTTAGGCATCTGGCGATATCGTGCAGTTCGAGAAGCCTTCCACTTCTTTTCATCCCATTATAAAACCTTACTTGCTTTCAACCTTGCTGAAAGTCTTCCTGGAGGAGACTCGATTTTGAAAACATTGTCTAGTCGATTGGTTATTTTAAATTCCAGTGCTTCCCAACACGATGTTTCTCCACATTCAAAACGGCAGTCAAAATTGTCTCCAATTTCACTTATAGCAATAACTCAACTTGGATTCATATTGCAAAAGGAAGTCAACCTTGATGGAGAGGTAATAATAGTTGGAGAGGTTTGTGCTGGTGAAAGTAACCCCTTGGAAATGGTAGTCAAACAATCATTATTGGACACTGGAAGGGCTGAAGCTGCACAGGCAACTGTGGACCAGGTGAAAGTGGATGGGATTGATACAAATGTTGCAATAATGAAGGTGATACTTTACTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF639

Protein Analysis

543

Amino Acids

60.47

Weight (kDa)

6.21

Isoelectric Point (pI)

42.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF639 PF04842 466 - 689 4.1e-62 Plant protein of unknown function (DUF639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1239
Acc36I ACCTGC 2 cut(s) 819, 934
AccII CGCG 1 cut(s) 571
AciI CCGC 4 cut(s) 88, 111, 135, 911
AclWI GGATC 1 cut(s) 36
AcsI RAATTY 4 cut(s) 7, 305, 1146, 1327
AcuI CTGAAG 3 cut(s) 246, 737, 1563
AfaI GTAC 5 cut(s) 727, 793, 979, 1007, 1164
AfiI CCNNNNNNNGG 1 cut(s) 703
AgsI TTSAA 9 cut(s) 13, 507, 720, 881, 1100, 1151, 1258, 1297, 1365
AjnI CCWGG 6 cut(s) 528, 580, 696, 841, 1276, 1566
AluBI AGCT 5 cut(s) 256, 277, 578, 757, 1547
AluI AGCT 5 cut(s) 256, 277, 578, 757, 1547
Alw21I GWGCWC 2 cut(s) 816, 1114
Alw26I GTCTC 4 cut(s) 196, 1068, 1278, 1389
AlwI GGATC 1 cut(s) 36
AoxI GGCC 3 cut(s) 554, 649, 839
ApaI GGGCCC 1 cut(s) 558
ApeKI GCWGC 4 cut(s) 93, 96, 590, 1547
ApoI RAATTY 4 cut(s) 7, 305, 1146, 1327
ArsI GACNNNNNNTTYG 2 cut(s) 1358, 1390
Asp700I GAANNNNTTC 1 cut(s) 1272
AspLEI GCGC 2 cut(s) 571, 871
AspS9I GGNCC 4 cut(s) 554, 555, 968, 1564
AsuHPI GGTGA 5 cut(s) 155, 368, 1496, 1582, 1624
AvaII GGWCC 2 cut(s) 968, 1564
BaeGI GKGCMC 1 cut(s) 558
BaeI ACNNNNGTAYC 2 cut(s) 1581, 1614
BanII GRGCYC 1 cut(s) 558
BarI GAAGNNNNNNTAC 4 cut(s) 261, 293, 718, 750
BbsI GAAGAC 2 cut(s) 71, 1265
Bbv12I GWGCWC 2 cut(s) 816, 1114
BbvCI CCTCAGC 1 cut(s) 762
BbvI GCAGC 4 cut(s) 105, 108, 577, 1534
BccI CCATC 8 cut(s) 32, 66, 479, 660, 782, 1007, 1442, 1574
BceAI ACGGC 2 cut(s) 863, 1385
BcgI CGANNNNNNTGC 2 cut(s) 548, 582
BciT130I CCWGG 6 cut(s) 530, 582, 698, 843, 1278, 1568
BcoDI GTCTC 4 cut(s) 196, 1068, 1278, 1389
BfaI CTAG 4 cut(s) 291, 456, 467, 1308
BfmI CTRYAG 1 cut(s) 1020
BfuAI ACCTGC 2 cut(s) 819, 934
BglI GCCNNNNNGGC 1 cut(s) 847
BisI GCNGC 5 cut(s) 94, 97, 112, 591, 1548
BlsI GCNGC 5 cut(s) 95, 98, 113, 592, 1549
BmcAI AGTACT 1 cut(s) 1164
Bme1390I CCNGG 6 cut(s) 530, 582, 698, 843, 1278, 1568
Bme18I GGWCC 2 cut(s) 968, 1564
BmgT120I GGNCC 4 cut(s) 554, 555, 968, 1564
BmiI GGNNCC 1 cut(s) 556
BmrFI CCNGG 6 cut(s) 530, 582, 698, 843, 1278, 1568
BmrI ACTGGG 1 cut(s) 26
BmsI GCATC 4 cut(s) 225, 247, 384, 1194
BmuI ACTGGG 1 cut(s) 26
BoxI GACNNNNGTC 1 cut(s) 850
BpiI GAAGAC 2 cut(s) 71, 1265
BpmI CTGGAG 1 cut(s) 1299
Bpu10I CCTNAGC 1 cut(s) 762
BpuEI CTTGAG 1 cut(s) 800
BsaJI CCNNGG 1 cut(s) 1497
Bsc4I CCNNNNNNNGG 1 cut(s) 703
Bse118I RCCGGY 1 cut(s) 1030
Bse1I ACTGG 6 cut(s) 21, 809, 970, 1170, 1332, 1537
BseBI CCWGG 6 cut(s) 530, 582, 698, 843, 1278, 1568
BseDI CCNNGG 1 cut(s) 1497
BseGI GGATG 7 cut(s) 27, 43, 216, 484, 774, 1229, 1585
BseLI CCNNNNNNNGG 1 cut(s) 703
BseMII CTCAG 3 cut(s) 411, 753, 1041
BseNI ACTGG 6 cut(s) 21, 809, 970, 1170, 1332, 1537
BseRI GAGGAG 1 cut(s) 1296
BseSI GKGCMC 1 cut(s) 558
BseXI GCAGC 4 cut(s) 105, 108, 577, 1534
BsgI GTGCAG 2 cut(s) 1221, 1533
Bsh1236I CGCG 1 cut(s) 571
BshFI GGCC 3 cut(s) 556, 651, 841
BsiHKAI GWGCWC 2 cut(s) 816, 1114
BsiSI CCGG 2 cut(s) 85, 1031
BslI CCNNNNNNNGG 1 cut(s) 703
BsmAI GTCTC 4 cut(s) 196, 1068, 1278, 1389
BsmBI CGTCTC 1 cut(s) 196
BsnI GGCC 3 cut(s) 556, 651, 841
Bsp120I GGGCCC 1 cut(s) 554
Bsp1286I GDGCHC 3 cut(s) 558, 816, 1114
Bsp1407I TGTACA 1 cut(s) 1005
Bsp143I GATC 2 cut(s) 28, 922
BspACI CCGC 4 cut(s) 88, 111, 135, 911
BspANI GGCC 3 cut(s) 556, 651, 841
BspCNI CTCAG 3 cut(s) 412, 754, 1042
BspFNI CGCG 1 cut(s) 571
BspLI GGNNCC 1 cut(s) 556
BspMI ACCTGC 2 cut(s) 819, 934
BspPI GGATC 1 cut(s) 36
BspQI GCTCTTC 1 cut(s) 1020
BsrFI RCCGGY 1 cut(s) 1030
BsrGI TGTACA 1 cut(s) 1005
BsrI ACTGG 6 cut(s) 21, 809, 970, 1170, 1332, 1537
BssAI RCCGGY 1 cut(s) 1030
BssECI CCNNGG 1 cut(s) 1497
BssMI GATC 2 cut(s) 28, 922
BssT1I CCWWGG 1 cut(s) 1497
Bst2UI CCWGG 6 cut(s) 530, 582, 698, 843, 1278, 1568
Bst4CI ACNGT 4 cut(s) 550, 613, 796, 1561
Bst6I CTCTTC 1 cut(s) 1020
BstAUI TGTACA 1 cut(s) 1005
BstC8I GCNNGC 3 cut(s) 399, 759, 1110
BstDEI CTNAG 3 cut(s) 420, 762, 1050
BstENI CCTNNNNNAGG 1 cut(s) 701
BstF5I GGATG 7 cut(s) 27, 43, 216, 484, 774, 1229, 1585
BstFNI CGCG 1 cut(s) 571
BstHHI GCGC 2 cut(s) 571, 871
BstKTI GATC 2 cut(s) 31, 925
BstMAI GTCTC 4 cut(s) 196, 1068, 1278, 1389
BstMBI GATC 2 cut(s) 28, 922
BstMWI GCNNNNNNNGC 7 cut(s) 575, 596, 648, 847, 1068, 1547, 1553
BstNI CCWGG 6 cut(s) 530, 582, 698, 843, 1278, 1568
BstPAI GACNNNNGTC 1 cut(s) 850
BstSCI CCNGG 6 cut(s) 528, 580, 696, 841, 1276, 1566
BstSFI CTRYAG 1 cut(s) 1020
BstSLI GKGCMC 1 cut(s) 558
BstUI CGCG 1 cut(s) 571
BstV1I GCAGC 4 cut(s) 105, 108, 577, 1534
BstV2I GAAGAC 2 cut(s) 71, 1265
BsuRI GGCC 3 cut(s) 556, 651, 841
BtsCI GGATG 7 cut(s) 27, 43, 216, 484, 774, 1229, 1585
BtsIMutI CAGTG 4 cut(s) 816, 854, 1339, 1530
BveI ACCTGC 2 cut(s) 819, 934
Cac8I GCNNGC 3 cut(s) 399, 759, 1110
CfoI GCGC 2 cut(s) 571, 871
Cfr10I RCCGGY 1 cut(s) 1030
Cfr13I GGNCC 4 cut(s) 554, 555, 968, 1564
CsiI ACCWGGT 1 cut(s) 1566
Csp6I GTAC 5 cut(s) 726, 792, 978, 1006, 1163
CviAII CATG 4 cut(s) 329, 391, 402, 1003
CviQI GTAC 5 cut(s) 726, 792, 978, 1006, 1163
DdeI CTNAG 3 cut(s) 420, 762, 1050
DpnI GATC 2 cut(s) 30, 924
DpnII GATC 2 cut(s) 28, 922
DraI TTTAAA 2 cut(s) 1057, 1326
Eam1104I CTCTTC 1 cut(s) 1020
EarI CTCTTC 1 cut(s) 1020
EciI GGCGGA 2 cut(s) 103, 124
Eco130I CCWWGG 1 cut(s) 1497
Eco24I GRGCYC 1 cut(s) 558
Eco32I GATATC 1 cut(s) 1196
Eco47I GGWCC 2 cut(s) 968, 1564
Eco57I CTGAAG 3 cut(s) 246, 737, 1563
EcoNI CCTNNNNNAGG 1 cut(s) 701
EcoRII CCWGG 6 cut(s) 528, 580, 696, 841, 1276, 1566
EcoRV GATATC 1 cut(s) 1196
EcoT14I CCWWGG 1 cut(s) 1497
EcoT38I GRGCYC 1 cut(s) 558
ErhI CCWWGG 1 cut(s) 1497
Esp3I CGTCTC 1 cut(s) 196
FaeI CATG 4 cut(s) 332, 394, 405, 1006
FalI AAGNNNNNCTT 2 cut(s) 1428, 1460
FatI CATG 4 cut(s) 328, 390, 401, 1002
Fnu4HI GCNGC 5 cut(s) 94, 97, 112, 591, 1548
FokI GGATG 7 cut(s) 34, 50, 203, 491, 761, 1216, 1592
FriOI GRGCYC 1 cut(s) 558
Fsp4HI GCNGC 5 cut(s) 94, 97, 112, 591, 1548
FspBI CTAG 4 cut(s) 291, 456, 467, 1308
GlaI GCGC 2 cut(s) 570, 870
GluI GCNGC 5 cut(s) 94, 97, 112, 591, 1548
GsuI CTGGAG 1 cut(s) 1299
HaeIII GGCC 3 cut(s) 556, 651, 841
HapII CCGG 2 cut(s) 85, 1031
HhaI GCGC 2 cut(s) 571, 871
Hin1II CATG 4 cut(s) 332, 394, 405, 1006
Hin6I GCGC 2 cut(s) 569, 869
HinP1I GCGC 2 cut(s) 569, 869
HincII GTYRAC 1 cut(s) 1441
HindII GTYRAC 1 cut(s) 1441
HinfI GANTC 9 cut(s) 67, 122, 143, 208, 407, 629, 881, 1286, 1419
HpaII CCGG 2 cut(s) 85, 1031
HphI GGTGA 5 cut(s) 155, 368, 1496, 1582, 1624
Hpy166II GTNNAC 3 cut(s) 616, 1441, 1564
Hpy188I TCNGA 6 cut(s) 63, 72, 142, 282, 1128, 1159
Hpy188III TCNNGA 4 cut(s) 46, 1049, 1097, 1208
Hpy8I GTNNAC 3 cut(s) 616, 1441, 1564
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 4 cut(s) 138, 1225, 1530, 1603
HpyCH4III ACNGT 4 cut(s) 550, 613, 796, 1561
HpyCH4IV ACGT 1 cut(s) 1016
HpyCH4V TGCA 6 cut(s) 401, 599, 1202, 1429, 1550, 1601
HpyF10VI GCNNNNNNNGC 7 cut(s) 575, 596, 648, 847, 1068, 1547, 1553
HpyF3I CTNAG 3 cut(s) 420, 762, 1050
HpySE526I ACGT 1 cut(s) 1016
Hsp92II CATG 4 cut(s) 332, 394, 405, 1006
HspAI GCGC 2 cut(s) 569, 869
Kzo9I GATC 2 cut(s) 28, 922
LguI GCTCTTC 1 cut(s) 1020
LmnI GCTCC 3 cut(s) 90, 274, 583
Lsp1109I GCAGC 4 cut(s) 105, 108, 577, 1534
LweI GCATC 4 cut(s) 225, 247, 384, 1194
MabI ACCWGGT 1 cut(s) 1566
MaeI CTAG 4 cut(s) 291, 456, 467, 1308
MaeII ACGT 1 cut(s) 1016
MaeIII GTNAC 4 cut(s) 374, 461, 852, 1490
MalI GATC 2 cut(s) 30, 924
MboI GATC 2 cut(s) 28, 922
MboII GAAGA 8 cut(s) 76, 128, 203, 239, 729, 743, 1037, 1265
MhlI GDGCHC 3 cut(s) 558, 816, 1114
MluCI AATT 9 cut(s) 7, 181, 230, 305, 1063, 1146, 1327, 1379, 1389
MlyI GAGTC 3 cut(s) 61, 116, 1280
MmeI TCCRAC 4 cut(s) 6, 15, 1065, 1447
MroXI GAANNNNTTC 1 cut(s) 1272
MseI TTAA 3 cut(s) 711, 1056, 1325
MspA1I CMGCKG 1 cut(s) 111
MspI CCGG 2 cut(s) 85, 1031
MspR9I CCNGG 6 cut(s) 530, 582, 698, 843, 1278, 1568
MvaI CCWGG 6 cut(s) 530, 582, 698, 843, 1278, 1568
MvnI CGCG 1 cut(s) 571
MwoI GCNNNNNNNGC 7 cut(s) 575, 596, 648, 847, 1068, 1547, 1553
NdeII GATC 2 cut(s) 28, 922
NlaIII CATG 4 cut(s) 332, 394, 405, 1006
NlaIV GGNNCC 1 cut(s) 556
NmeAIII GCCGAG 1 cut(s) 1097
NmuCI GTSAC 2 cut(s) 374, 852
PciSI GCTCTTC 1 cut(s) 1020
PcsI WCGNNNNNNNCGW 2 cut(s) 183, 1204
PdmI GAANNNNTTC 1 cut(s) 1272
PfeI GAWTC 6 cut(s) 143, 208, 407, 629, 881, 1419
PflFI GACNNNGTC 1 cut(s) 704
PfoI TCCNGGA 1 cut(s) 1276
PkrI GCNGC 5 cut(s) 95, 98, 113, 592, 1549
PleI GAGTC 3 cut(s) 61, 116, 1280
PpsI GAGTC 3 cut(s) 61, 116, 1280
PshAI GACNNNNGTC 1 cut(s) 850
PsiI TTATAA 1 cut(s) 1239
Psp6I CCWGG 6 cut(s) 528, 580, 696, 841, 1276, 1566
PspGI CCWGG 6 cut(s) 528, 580, 696, 841, 1276, 1566
PspN4I GGNNCC 1 cut(s) 556
PspOMI GGGCCC 1 cut(s) 554
PspPI GGNCC 4 cut(s) 554, 555, 968, 1564
PsyI GACNNNGTC 1 cut(s) 704
RsaI GTAC 5 cut(s) 727, 793, 979, 1007, 1164
RsaNI GTAC 5 cut(s) 726, 792, 978, 1006, 1163
SapI GCTCTTC 1 cut(s) 1020
SaqAI TTAA 3 cut(s) 711, 1056, 1325
SatI GCNGC 5 cut(s) 94, 97, 112, 591, 1548
Sau3AI GATC 2 cut(s) 28, 922
Sau96I GGNCC 4 cut(s) 554, 555, 968, 1564
ScaI AGTACT 1 cut(s) 1164
SchI GAGTC 3 cut(s) 61, 116, 1280
ScrFI CCNGG 6 cut(s) 530, 582, 698, 843, 1278, 1568
SduI GDGCHC 3 cut(s) 558, 816, 1114
SexAI ACCWGGT 1 cut(s) 1566
SfaNI GCATC 4 cut(s) 225, 247, 384, 1194
SfcI CTRYAG 1 cut(s) 1020
SinI GGWCC 2 cut(s) 968, 1564
SmlI CTYRAG 1 cut(s) 815
SmoI CTYRAG 1 cut(s) 815
Sse9I AATT 9 cut(s) 7, 181, 230, 305, 1063, 1146, 1327, 1379, 1389
SsiI CCGC 4 cut(s) 88, 111, 135, 911
SspMI CTAG 4 cut(s) 291, 456, 467, 1308
StyD4I CCNGG 6 cut(s) 528, 580, 696, 841, 1276, 1566
StyI CCWWGG 1 cut(s) 1497
TaaI ACNGT 4 cut(s) 550, 613, 796, 1561
TaiI ACGT 1 cut(s) 1019
TaqI TCGA 4 cut(s) 658, 1207, 1289, 1312
TasI AATT 9 cut(s) 7, 181, 230, 305, 1063, 1146, 1327, 1379, 1389
TatI WGTACW 3 cut(s) 725, 1005, 1162
TauI GCSGC 1 cut(s) 114
TfiI GAWTC 6 cut(s) 143, 208, 407, 629, 881, 1419
Tru1I TTAA 3 cut(s) 711, 1056, 1325
Tru9I TTAA 3 cut(s) 711, 1056, 1325
TscAI CASTG 4 cut(s) 816, 861, 1339, 1537
TseFI GTSAC 2 cut(s) 374, 852
TseI GCWGC 4 cut(s) 93, 96, 590, 1547
Tsp45I GTSAC 2 cut(s) 374, 852
TspDTI ATGAA 7 cut(s) 653, 953, 1025, 1111, 1218, 1411, 1622
TspGWI ACGGA 1 cut(s) 175
TspRI CASTG 4 cut(s) 816, 861, 1339, 1537
Tth111I GACNNNGTC 1 cut(s) 704
VpaK11BI GGWCC 2 cut(s) 968, 1564
XagI CCTNNNNNAGG 1 cut(s) 701
XapI RAATTY 4 cut(s) 7, 305, 1146, 1327
XcmI CCANNNNNNNNNTGG 1 cut(s) 335
XmnI GAANNNNTTC 1 cut(s) 1272
XspI CTAG 4 cut(s) 291, 456, 467, 1308
ZrmI AGTACT 1 cut(s) 1164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.