Rh4CG466000

Plant protein of unknown function (DUF639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0005385
Physical Location & Seq
Reverse (-)
69573315 .. 69579719
6405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG466000.1

Sequence Viewer

Length: 1677 bp
ATGGCTGTCCAGGTTGATGACAAAAAAACTGTTGGGCCCGAAGCCTTTGCACGAATAGCACCTGGTTGTGCTGCTGTTGCAGATATAATAACTGTCCACAACCTCTATGATGCACTCACAAGCACATCAGGCCATCATCGACTTCATTTCCTTGTATATGACAAATACATAAGAAGCCTAGACAAGGTTATTAAGGCTTCCAAAAGTACATTAGCTTCTTCCATTGGAAATCTTCAGCTCGTTGAGGGAGAAATCATCCTTGATGTTGATGGTACAGTTCCCACTCAACCAGTGCTCAAACATATTGGCAGGTCTATGTGGCCTGGACGGCTGTCACTGACCAATAGTGCGCTGTATTTTGAATCATTGGGAGTTGGTTTGTATGACAAAGCTGTTAGATATGATCTGGCAACAGATATGAAGCAGGTCATAAAACCAGAATTGACTGGACCGTTGGGTACTCGTCTTTTCGATAAAGCCATCATGTACAAATCGACGTCTGTAGAAGAGCCGGTCCATTTGGAGTTTCCTGAGTTCAAAGGCAATTCTCGCCGAGATTATTGGTTGGATATTTGTCTTGAAATCTTGCGTGCTCACAGGTTCATTCAGAAAAACAACCTTAAAGCAATTCAGAAATCAGAAGTACTGGCGAGGGCTATTTTAGGCATCTGGCGATATCGTGCAGTTCGAGAAGCCTTCCACTTCTTTTCATCCCATTATAAAACCTTACTTGCTTTCAACCTTGCTGAATGTCTTCCGGGAGGAGACTCGATTTTGAAAACTTTGTCTAGTCGATTGGTTATTTTGAATTCCAGTGCTTCCCAACATGATGTTTCTCCACATTCAAAACGGCAGGCAAAATTGTCTCCAATTTCACTTATAGCACTAACTCAGTTTGGATTCATATTGCAAAAGGAAGTCAACCTTGATGGAGAGGTAATAATAGTTGGAGAGGTTTGTGCCGGTGAAATTAACCCCTTGCAAATGGCAGTGAAACAGTCATTATTGGACACTGGAAGGGCTGAAGCTGCACAGGCAACTGTGGACCAAGTGAAAGTGGATGGGATTGATACAAATGTTGCAATAATGAAGGAGTTACTGTTTCCGGTTATTGAATTAGCTAATTGTGCTCAACGTTTGGCATCATGGGAAAAGCCTTACAAGTCAACAATTTTTCTGGTGTTGACCTGCTTCTTTATAGTAAGGGGCTGGTTCAGTTATGTATTGCCCTCCATTTTTGTATTGGTAGCAGTTGTCATGCTCTGGTGCAGGCATTTCAACAAAGGAAAACCATTAAAACCATTCAGGATTACACCTCCCCATAACAATGCAGTAGAGCAGCTGCTGACATTACAAGAAGCCATCACTCAAGTTGAAGCATTACTTCGAGCTGGGAATATTATCCTACTGAAGATAAGAGCCCTCCTGTTTGCAGTTCTTCCTCAGGCATCGGACAAGATTGTTATACTGCTGGTTTTCATGGCAGCAATGTTTGCATTTGTGCCTCCGCGATATATAATCCTGCTAGTATTTCTGGAGGCTTATACAAGGGAAATGCCATATAGGAAAGAAAGCAGTGACAGGTGGGTGAGGCGGATTAGAGAATGGTGGATTCGCATACCAGCAGCTCCTGTCCAGCTCATCAAACCTGAGGATAACAAGAAAAAGAAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF639

Protein Analysis

558

Amino Acids

62.86

Weight (kDa)

9.38

Isoelectric Point (pI)

37.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF639 PF04842 324 - 548 8.3e-63 Plant protein of unknown function (DUF639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 720
AatII GACGTC 1 cut(s) 500
Acc36I ACCTGC 3 cut(s) 300, 415, 1196
AccII CGCG 1 cut(s) 1510
AciI CCGC 2 cut(s) 1508, 1594
AclI AACGTT 1 cut(s) 1135
AcsI RAATTY 1 cut(s) 808
AcuI CTGAAG 3 cut(s) 218, 1044, 1430
AcyI GRCGYC 1 cut(s) 497
AfaI GTAC 5 cut(s) 208, 274, 460, 488, 645
AfiI CCNNNNNNNGG 1 cut(s) 184
AjnI CCWGG 3 cut(s) 9, 61, 322
AluBI AGCT 9 cut(s) 215, 238, 392, 1028, 1121, 1342, 1391, 1628, 1639
AluI AGCT 9 cut(s) 215, 238, 392, 1028, 1121, 1342, 1391, 1628, 1639
Alw21I GWGCWC 3 cut(s) 297, 595, 1132
Alw26I GTCTC 2 cut(s) 759, 870
AlwNI CAGNNNCTG 2 cut(s) 1345, 1631
AoxI GGCC 3 cut(s) 35, 130, 320
ApaI GGGCCC 1 cut(s) 39
ApeKI GCWGC 6 cut(s) 71, 1028, 1339, 1342, 1484, 1625
ApoI RAATTY 1 cut(s) 808
Asp700I GAANNNNTTC 1 cut(s) 753
AspLEI GCGC 1 cut(s) 352
AspS9I GGNCC 5 cut(s) 35, 36, 449, 514, 1045
AsuC2I CCSGG 1 cut(s) 759
AsuHPI GGTGA 2 cut(s) 977, 1600
AvaII GGWCC 3 cut(s) 449, 514, 1045
AxyI CCTNAGG 2 cut(s) 1443, 1650
BaeGI GKGCMC 1 cut(s) 39
BaeI ACNNNNGTAYC 2 cut(s) 1062, 1095
BanII GRGCYC 2 cut(s) 39, 1423
BarI GAAGNNNNNNTAC 2 cut(s) 199, 231
BbsI GAAGAC 1 cut(s) 746
Bbv12I GWGCWC 3 cut(s) 297, 595, 1132
BbvI GCAGC 6 cut(s) 58, 1015, 1329, 1351, 1496, 1637
BccI CCATC 6 cut(s) 141, 263, 488, 923, 1055, 1370
BceAI ACGGC 2 cut(s) 344, 866
BcgI CGANNNNNNTGC 2 cut(s) 29, 63
BciT130I CCWGG 3 cut(s) 11, 63, 324
BcnI CCSGG 1 cut(s) 759
BcoDI GTCTC 2 cut(s) 759, 870
BfaI CTAG 3 cut(s) 179, 789, 1526
BfmI CTRYAG 1 cut(s) 501
BfuAI ACCTGC 3 cut(s) 300, 415, 1196
BglI GCCNNNNNGGC 1 cut(s) 328
BisI GCNGC 6 cut(s) 72, 1029, 1340, 1343, 1485, 1626
BlsI GCNGC 6 cut(s) 73, 1030, 1341, 1344, 1486, 1627
BmcAI AGTACT 1 cut(s) 645
Bme1390I CCNGG 4 cut(s) 11, 63, 324, 759
Bme18I GGWCC 3 cut(s) 449, 514, 1045
BmgT120I GGNCC 5 cut(s) 35, 36, 449, 514, 1045
BmiI GGNNCC 1 cut(s) 37
BmrFI CCNGG 4 cut(s) 11, 63, 324, 759
BmsI GCATC 4 cut(s) 100, 675, 1151, 1457
BoxI GACNNNNGTC 1 cut(s) 331
BpiI GAAGAC 1 cut(s) 746
BpmI CTGGAG 1 cut(s) 1556
BpuEI CTTGAG 1 cut(s) 1353
BpuMI CCSGG 1 cut(s) 759
BsaHI GRCGYC 1 cut(s) 497
BsaWI WCCGGW 1 cut(s) 1105
BsaXI ACNNNNNCTCC 2 cut(s) 1085, 1115
Bsc4I CCNNNNNNNGG 1 cut(s) 184
Bse118I RCCGGY 2 cut(s) 511, 962
Bse1I ACTGG 5 cut(s) 290, 451, 651, 813, 1018
Bse21I CCTNAGG 2 cut(s) 1443, 1650
Bse3DI GCAATG 1 cut(s) 1494
BseBI CCWGG 3 cut(s) 11, 63, 324
BseGI GGATG 3 cut(s) 255, 710, 1066
BseLI CCNNNNNNNGG 1 cut(s) 184
BseMI GCAATG 1 cut(s) 1494
BseMII CTCAG 4 cut(s) 522, 905, 1457, 1641
BseNI ACTGG 5 cut(s) 290, 451, 651, 813, 1018
BseRI GAGGAG 1 cut(s) 777
BseSI GKGCMC 1 cut(s) 39
BseXI GCAGC 6 cut(s) 58, 1015, 1329, 1351, 1496, 1637
BseYI CCCAGC 1 cut(s) 1391
BsgI GTGCAG 3 cut(s) 702, 1014, 1288
Bsh1236I CGCG 1 cut(s) 1510
BshFI GGCC 3 cut(s) 37, 132, 322
BsiHKAI GWGCWC 3 cut(s) 297, 595, 1132
BsiSI CCGG 4 cut(s) 512, 758, 963, 1106
BslI CCNNNNNNNGG 1 cut(s) 184
BsmAI GTCTC 2 cut(s) 759, 870
BsnI GGCC 3 cut(s) 37, 132, 322
Bsp120I GGGCCC 1 cut(s) 35
Bsp1286I GDGCHC 5 cut(s) 39, 297, 595, 1132, 1423
Bsp1407I TGTACA 1 cut(s) 486
Bsp143I GATC 1 cut(s) 403
BspACI CCGC 2 cut(s) 1508, 1594
BspANI GGCC 3 cut(s) 37, 132, 322
BspCNI CTCAG 4 cut(s) 523, 904, 1456, 1642
BspFNI CGCG 1 cut(s) 1510
BspLI GGNNCC 1 cut(s) 37
BspMI ACCTGC 3 cut(s) 300, 415, 1196
BspQI GCTCTTC 1 cut(s) 501
BsrDI GCAATG 1 cut(s) 1494
BsrFI RCCGGY 2 cut(s) 511, 962
BsrGI TGTACA 1 cut(s) 486
BsrI ACTGG 5 cut(s) 290, 451, 651, 813, 1018
BssAI RCCGGY 2 cut(s) 511, 962
BssMI GATC 1 cut(s) 403
BssNI GRCGYC 1 cut(s) 497
Bst2UI CCWGG 3 cut(s) 11, 63, 324
Bst4CI ACNGT 7 cut(s) 31, 94, 277, 453, 999, 1042, 1101
Bst6I CTCTTC 1 cut(s) 501
BstACI GRCGYC 1 cut(s) 497
BstAPI GCANNNNNTGC 1 cut(s) 1493
BstAUI TGTACA 1 cut(s) 486
BstC8I GCNNGC 3 cut(s) 591, 855, 1271
BstDEI CTNAG 4 cut(s) 531, 891, 1443, 1650
BstENI CCTNNNNNAGG 1 cut(s) 182
BstF5I GGATG 3 cut(s) 255, 710, 1066
BstFNI CGCG 1 cut(s) 1510
BstHHI GCGC 1 cut(s) 352
BstKTI GATC 1 cut(s) 406
BstMAI GTCTC 2 cut(s) 759, 870
BstMBI GATC 1 cut(s) 403
BstMWI GCNNNNNNNGC 9 cut(s) 56, 77, 129, 328, 549, 1028, 1034, 1127, 1493
BstNI CCWGG 3 cut(s) 11, 63, 324
BstPAI GACNNNNGTC 1 cut(s) 331
BstSCI CCNGG 4 cut(s) 9, 61, 322, 757
BstSFI CTRYAG 1 cut(s) 501
BstSLI GKGCMC 1 cut(s) 39
BstUI CGCG 1 cut(s) 1510
BstV1I GCAGC 6 cut(s) 58, 1015, 1329, 1351, 1496, 1637
BstV2I GAAGAC 1 cut(s) 746
Bsu36I CCTNAGG 2 cut(s) 1443, 1650
BsuRI GGCC 3 cut(s) 37, 132, 322
BtsCI GGATG 3 cut(s) 255, 710, 1066
BtsI GCAGTG 2 cut(s) 996, 1582
BtsIMutI CAGTG 6 cut(s) 297, 335, 820, 996, 1011, 1582
BveI ACCTGC 3 cut(s) 300, 415, 1196
Cac8I GCNNGC 3 cut(s) 591, 855, 1271
CaiI CAGNNNCTG 2 cut(s) 1345, 1631
CfoI GCGC 1 cut(s) 352
Cfr10I RCCGGY 2 cut(s) 511, 962
Cfr13I GGNCC 5 cut(s) 35, 36, 449, 514, 1045
CsiI ACCWGGT 1 cut(s) 61
Csp6I GTAC 5 cut(s) 207, 273, 459, 487, 644
CviAII CATG 5 cut(s) 484, 827, 1146, 1258, 1480
CviQI GTAC 5 cut(s) 207, 273, 459, 487, 644
DdeI CTNAG 4 cut(s) 531, 891, 1443, 1650
DpnI GATC 1 cut(s) 405
DpnII GATC 1 cut(s) 403
Eam1104I CTCTTC 1 cut(s) 501
EarI CTCTTC 1 cut(s) 501
EciI GGCGGA 1 cut(s) 1609
Eco24I GRGCYC 2 cut(s) 39, 1423
Eco32I GATATC 1 cut(s) 677
Eco47I GGWCC 3 cut(s) 449, 514, 1045
Eco57I CTGAAG 3 cut(s) 218, 1044, 1430
Eco81I CCTNAGG 2 cut(s) 1443, 1650
EcoNI CCTNNNNNAGG 1 cut(s) 182
EcoRI GAATTC 1 cut(s) 808
EcoRII CCWGG 3 cut(s) 9, 61, 322
EcoRV GATATC 1 cut(s) 677
EcoT38I GRGCYC 2 cut(s) 39, 1423
FaeI CATG 5 cut(s) 487, 830, 1149, 1261, 1483
FalI AAGNNNNNCTT 4 cut(s) 909, 941, 1368, 1400
FatI CATG 5 cut(s) 483, 826, 1145, 1257, 1479
Fnu4HI GCNGC 6 cut(s) 72, 1029, 1340, 1343, 1485, 1626
FokI GGATG 3 cut(s) 242, 697, 1073
FriOI GRGCYC 2 cut(s) 39, 1423
Fsp4HI GCNGC 6 cut(s) 72, 1029, 1340, 1343, 1485, 1626
FspBI CTAG 3 cut(s) 179, 789, 1526
GlaI GCGC 1 cut(s) 351
GluI GCNGC 6 cut(s) 72, 1029, 1340, 1343, 1485, 1626
GsaI CCCAGC 1 cut(s) 1395
GsuI CTGGAG 1 cut(s) 1556
HaeIII GGCC 3 cut(s) 37, 132, 322
HapII CCGG 4 cut(s) 512, 758, 963, 1106
HhaI GCGC 1 cut(s) 352
Hin1I GRCGYC 1 cut(s) 497
Hin1II CATG 5 cut(s) 487, 830, 1149, 1261, 1483
Hin6I GCGC 1 cut(s) 350
HinP1I GCGC 1 cut(s) 350
HincII GTYRAC 3 cut(s) 922, 1167, 1185
HindII GTYRAC 3 cut(s) 922, 1167, 1185
HinfI GANTC 4 cut(s) 362, 767, 900, 1612
HpaII CCGG 4 cut(s) 512, 758, 963, 1106
HphI GGTGA 2 cut(s) 977, 1600
Hpy166II GTNNAC 5 cut(s) 97, 922, 1045, 1167, 1185
Hpy188I TCNGA 4 cut(s) 609, 633, 640, 1453
Hpy188III TCNNGA 5 cut(s) 530, 578, 689, 1306, 1535
Hpy8I GTNNAC 5 cut(s) 97, 922, 1045, 1167, 1185
Hpy99I CGWCG 1 cut(s) 499
HpyAV CCTTC 3 cut(s) 706, 1011, 1084
HpyCH4III ACNGT 7 cut(s) 31, 94, 277, 453, 999, 1042, 1101
HpyCH4IV ACGT 2 cut(s) 497, 1135
HpyF10VI GCNNNNNNNGC 9 cut(s) 56, 77, 129, 328, 549, 1028, 1034, 1127, 1493
HpyF3I CTNAG 4 cut(s) 531, 891, 1443, 1650
HpySE526I ACGT 2 cut(s) 497, 1135
Hsp92I GRCGYC 1 cut(s) 497
Hsp92II CATG 5 cut(s) 487, 830, 1149, 1261, 1483
HspAI GCGC 1 cut(s) 350
Kzo9I GATC 1 cut(s) 403
LguI GCTCTTC 1 cut(s) 501
LmnI GCTCC 1 cut(s) 1633
Lsp1109I GCAGC 6 cut(s) 58, 1015, 1329, 1351, 1496, 1637
LweI GCATC 4 cut(s) 100, 675, 1151, 1457
MabI ACCWGGT 1 cut(s) 61
MaeI CTAG 3 cut(s) 179, 789, 1526
MaeII ACGT 2 cut(s) 497, 1135
MaeIII GTNAC 3 cut(s) 333, 1095, 1577
MalI GATC 1 cut(s) 405
MboI GATC 1 cut(s) 403
MboII GAAGA 6 cut(s) 210, 224, 518, 746, 1423, 1430
MhlI GDGCHC 5 cut(s) 39, 297, 595, 1132, 1423
MlyI GAGTC 1 cut(s) 761
MmeI TCCRAC 2 cut(s) 546, 928
MroXI GAANNNNTTC 1 cut(s) 753
MseI TTAA 4 cut(s) 192, 621, 972, 1295
MslI CAYNNNNRTG 1 cut(s) 1326
MspA1I CMGCKG 1 cut(s) 1342
MspI CCGG 4 cut(s) 512, 758, 963, 1106
MspR9I CCNGG 4 cut(s) 11, 63, 324, 759
MvaI CCWGG 3 cut(s) 11, 63, 324
MvnI CGCG 1 cut(s) 1510
MwoI GCNNNNNNNGC 9 cut(s) 56, 77, 129, 328, 549, 1028, 1034, 1127, 1493
NciI CCSGG 1 cut(s) 759
NdeII GATC 1 cut(s) 403
NlaIII CATG 5 cut(s) 487, 830, 1149, 1261, 1483
NlaIV GGNNCC 1 cut(s) 37
NmeAIII GCCGAG 1 cut(s) 578
NmuCI GTSAC 2 cut(s) 333, 1577
PciSI GCTCTTC 1 cut(s) 501
PcsI WCGNNNNNNNCGW 1 cut(s) 685
PdmI GAANNNNTTC 1 cut(s) 753
PfeI GAWTC 3 cut(s) 362, 900, 1612
PfoI TCCNGGA 1 cut(s) 757
PkrI GCNGC 6 cut(s) 73, 1030, 1341, 1344, 1486, 1627
PleI GAGTC 1 cut(s) 761
PpsI GAGTC 1 cut(s) 761
PshAI GACNNNNGTC 1 cut(s) 331
PsiI TTATAA 1 cut(s) 720
Psp1406I AACGTT 1 cut(s) 1135
Psp6I CCWGG 3 cut(s) 9, 61, 322
PspFI CCCAGC 1 cut(s) 1391
PspGI CCWGG 3 cut(s) 9, 61, 322
PspN4I GGNNCC 1 cut(s) 37
PspOMI GGGCCC 1 cut(s) 35
PspPI GGNCC 5 cut(s) 35, 36, 449, 514, 1045
PstNI CAGNNNCTG 2 cut(s) 1345, 1631
PvuII CAGCTG 1 cut(s) 1342
RsaI GTAC 5 cut(s) 208, 274, 460, 488, 645
RsaNI GTAC 5 cut(s) 207, 273, 459, 487, 644
RseI CAYNNNNRTG 1 cut(s) 1326
SapI GCTCTTC 1 cut(s) 501
SaqAI TTAA 4 cut(s) 192, 621, 972, 1295
SatI GCNGC 6 cut(s) 72, 1029, 1340, 1343, 1485, 1626
Sau3AI GATC 1 cut(s) 403
Sau96I GGNCC 5 cut(s) 35, 36, 449, 514, 1045
ScaI AGTACT 1 cut(s) 645
SchI GAGTC 1 cut(s) 761
ScrFI CCNGG 4 cut(s) 11, 63, 324, 759
SduI GDGCHC 5 cut(s) 39, 297, 595, 1132, 1423
SexAI ACCWGGT 1 cut(s) 61
SfaNI GCATC 4 cut(s) 100, 675, 1151, 1457
SfcI CTRYAG 1 cut(s) 501
SinI GGWCC 3 cut(s) 449, 514, 1045
SmiMI CAYNNNNRTG 1 cut(s) 1326
SmlI CTYRAG 1 cut(s) 1368
SmoI CTYRAG 1 cut(s) 1368
SsiI CCGC 2 cut(s) 1508, 1594
SspI AATATT 1 cut(s) 1399
SspMI CTAG 3 cut(s) 179, 789, 1526
StyD4I CCNGG 4 cut(s) 9, 61, 322, 757
TaaI ACNGT 7 cut(s) 31, 94, 277, 453, 999, 1042, 1101
TaiI ACGT 2 cut(s) 500, 1138
TaqI TCGA 7 cut(s) 139, 471, 494, 688, 770, 793, 1387
TatI WGTACW 3 cut(s) 206, 486, 643
TfiI GAWTC 3 cut(s) 362, 900, 1612
Tru1I TTAA 4 cut(s) 192, 621, 972, 1295
Tru9I TTAA 4 cut(s) 192, 621, 972, 1295
TscAI CASTG 6 cut(s) 297, 342, 820, 996, 1018, 1582
TseFI GTSAC 2 cut(s) 333, 1577
TseI GCWGC 6 cut(s) 71, 1028, 1339, 1342, 1484, 1625
Tsp45I GTSAC 2 cut(s) 333, 1577
TspDTI ATGAA 7 cut(s) 134, 434, 592, 699, 892, 1103, 1468
TspRI CASTG 6 cut(s) 297, 342, 820, 996, 1018, 1582
VpaK11BI GGWCC 3 cut(s) 449, 514, 1045
XagI CCTNNNNNAGG 1 cut(s) 182
XapI RAATTY 1 cut(s) 808
XcmI CCANNNNNNNNNTGG 1 cut(s) 1240
XmnI GAANNNNTTC 1 cut(s) 753
XspI CTAG 3 cut(s) 179, 789, 1526
ZraI GACGTC 1 cut(s) 498
ZrmI AGTACT 1 cut(s) 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.