FvH4_5g00420

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0017295
Physical Location & Seq
Reverse (-)
282932 .. 283525
594 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g00420.t1

Sequence Viewer

Length: 594 bp
ATGGAATGCCATCTGATGCTAGCTCTGCCTATTCTCCAGTTGCTTCTTAACCACATAAACCCCTGCACAGCTGCTGCAGCATCTTCTAGCAAAGTGAACACCGAGTACATAAAGACATCATGCAGCGTCACGCTCTATCCGAAACTGTGCTACACCTCCCTCTCAATCTATGCTGGAAAAATCAAGACCAGCCCGAAAGTGCTAGCCCATACCGCCCTCAACGTGACCCTTAGAGCCACCAAAGCAACTTCCGCGGTAATAAACAGGTGTTCAAAAGTTCATGGACTGAAGCCTATAGAGGTTGCAGCCACACTCGACTGTGTGGAGGAGATTAGTGATGCTTTGGATGAGCTGCGAAAGTCCATAGGTGAATTGGGTCATGCAGTTAAAGGCTCGAGAAGTTTCTTGTTTCAAATTAGTGATATTCAAACATGGGTTAGTGCTGCATTAACTGATGAGGACACCTGCTTGGATGGTTTTGATGAACCTAAATTGAATGGAAGGGTGGAGACTATTGTCCGGAGGGAAATAACGAAGATCGCGCATCTGACGAGCAATGCCTTGGCGCTCATCAACAACTACGCCTCAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

198

Amino Acids

21.37

Weight (kDa)

7.61

Isoelectric Point (pI)

35.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 36 - 190 2.5e-37 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017295)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g00420
malus_domestica MD06G1064700.v1.1
prunus_persica Prupe.5G076800_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0202091
rosa_laevigata RLG00000003626
rosa_multiflora Rmu_sc0006592.1_g000014
rosa_roxburghii Rroxscaffold_3G00254880
rosa_rugosa Rorug07G0067900
rosa_samantha Rh7AG195400 Rh7BG196700 Rh7CG206500 Rh7DG202000
rosa_wichuraiana Rw7G017110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 473
Acc36I ACCTGC 1 cut(s) 473
AccII CGCG 2 cut(s) 254, 542
AccIII TCCGGA 1 cut(s) 519
AciI CCGC 3 cut(s) 213, 252, 254
AcuI CTGAAG 1 cut(s) 308
AfaI GTAC 1 cut(s) 107
AgsI TTSAA 4 cut(s) 273, 413, 428, 496
AluBI AGCT 3 cut(s) 23, 71, 352
AluI AGCT 3 cut(s) 23, 71, 352
Alw26I GTCTC 1 cut(s) 503
AlwNI CAGNNNCTG 1 cut(s) 74
Ama87I CYCGRG 1 cut(s) 394
Aor13HI TCCGGA 1 cut(s) 519
ApeKI GCWGC 7 cut(s) 71, 74, 77, 123, 305, 352, 443
AspLEI GCGC 2 cut(s) 544, 568
AsuHPI GGTGA 1 cut(s) 380
AsuNHI GCTAGC 2 cut(s) 19, 202
AvaI CYCGRG 1 cut(s) 394
BbvI GCAGC 7 cut(s) 58, 61, 89, 135, 317, 339, 430
BccI CCATC 2 cut(s) 18, 467
BcoDI GTCTC 1 cut(s) 503
BfaI CTAG 3 cut(s) 20, 87, 203
BfmI CTRYAG 2 cut(s) 75, 294
BfoI RGCGCY 1 cut(s) 569
BfuAI ACCTGC 1 cut(s) 473
BisI GCNGC 7 cut(s) 72, 75, 78, 124, 306, 353, 444
BlsI GCNGC 7 cut(s) 73, 76, 79, 125, 307, 354, 445
BmeT110I CYCGRG 1 cut(s) 394
BmsI GCATC 4 cut(s) 6, 89, 328, 553
BmtI GCTAGC 2 cut(s) 23, 206
BoxI GACNNNNGTC 1 cut(s) 515
BpmI CTGGAG 1 cut(s) 20
BsaJI CCNNGG 2 cut(s) 252, 561
BsaWI WCCGGW 1 cut(s) 519
Bse1I ACTGG 1 cut(s) 37
Bse3DI GCAATG 1 cut(s) 562
BseAI TCCGGA 1 cut(s) 519
BseDI CCNNGG 2 cut(s) 252, 561
BseGI GGATG 2 cut(s) 352, 478
BseMI GCAATG 1 cut(s) 562
BseNI ACTGG 1 cut(s) 37
BseRI GAGGAG 1 cut(s) 341
BseXI GCAGC 7 cut(s) 58, 61, 89, 135, 317, 339, 430
BsgI GTGCAG 1 cut(s) 49
Bsh1236I CGCG 2 cut(s) 254, 542
BsiHKCI CYCGRG 1 cut(s) 394
BsiSI CCGG 1 cut(s) 520
BsmAI GTCTC 1 cut(s) 503
BsmI GAATGC 1 cut(s) 11
BsoBI CYCGRG 1 cut(s) 394
Bsp13I TCCGGA 1 cut(s) 519
Bsp143I GATC 1 cut(s) 537
BspACI CCGC 3 cut(s) 213, 252, 254
BspEI TCCGGA 1 cut(s) 519
BspFNI CGCG 2 cut(s) 254, 542
BspMAI CTGCAG 1 cut(s) 79
BspMI ACCTGC 1 cut(s) 473
BspOI GCTAGC 2 cut(s) 23, 206
BsrDI GCAATG 1 cut(s) 562
BsrI ACTGG 1 cut(s) 37
BssECI CCNNGG 2 cut(s) 252, 561
BssMI GATC 1 cut(s) 537
BssT1I CCWWGG 1 cut(s) 561
Bst4CI ACNGT 2 cut(s) 147, 320
BstC8I GCNNGC 2 cut(s) 21, 204
BstDEI CTNAG 1 cut(s) 230
BstDSI CCRYGG 1 cut(s) 252
BstF5I GGATG 2 cut(s) 352, 478
BstFNI CGCG 2 cut(s) 254, 542
BstH2I RGCGCY 1 cut(s) 569
BstHHI GCGC 2 cut(s) 544, 568
BstKTI GATC 1 cut(s) 540
BstMAI GTCTC 1 cut(s) 503
BstMBI GATC 1 cut(s) 537
BstMWI GCNNNNNNNGC 5 cut(s) 25, 77, 212, 242, 251
BstPAI GACNNNNGTC 1 cut(s) 515
BstSFI CTRYAG 2 cut(s) 75, 294
BstUI CGCG 2 cut(s) 254, 542
BstV1I GCAGC 7 cut(s) 58, 61, 89, 135, 317, 339, 430
BtgI CCRYGG 1 cut(s) 252
BtsCI GGATG 2 cut(s) 352, 478
BveI ACCTGC 1 cut(s) 473
Cac8I GCNNGC 2 cut(s) 21, 204
CaiI CAGNNNCTG 1 cut(s) 74
CfoI GCGC 2 cut(s) 544, 568
Cfr42I CCGCGG 1 cut(s) 255
CseI GACGC 1 cut(s) 115
Csp6I GTAC 1 cut(s) 106
CviAII CATG 4 cut(s) 120, 281, 380, 432
CviJI RGCY 9 cut(s) 23, 71, 192, 206, 236, 292, 308, 352, 393
CviKI_1 RGCY 9 cut(s) 23, 71, 192, 206, 236, 292, 308, 352, 393
CviQI GTAC 1 cut(s) 106
DdeI CTNAG 1 cut(s) 230
DpnI GATC 1 cut(s) 539
DpnII GATC 1 cut(s) 537
Eco130I CCWWGG 1 cut(s) 561
Eco57I CTGAAG 1 cut(s) 308
Eco88I CYCGRG 1 cut(s) 394
EcoT14I CCWWGG 1 cut(s) 561
ErhI CCWWGG 1 cut(s) 561
FaeI CATG 4 cut(s) 123, 284, 383, 435
FatI CATG 4 cut(s) 119, 280, 379, 431
Fnu4HI GCNGC 7 cut(s) 72, 75, 78, 124, 306, 353, 444
FokI GGATG 2 cut(s) 359, 485
Fsp4HI GCNGC 7 cut(s) 72, 75, 78, 124, 306, 353, 444
FspBI CTAG 3 cut(s) 20, 87, 203
GlaI GCGC 2 cut(s) 543, 567
GluI GCNGC 7 cut(s) 72, 75, 78, 124, 306, 353, 444
GsuI CTGGAG 1 cut(s) 20
HaeII RGCGCY 1 cut(s) 569
HapII CCGG 1 cut(s) 520
HgaI GACGC 1 cut(s) 115
HhaI GCGC 2 cut(s) 544, 568
Hin1II CATG 4 cut(s) 123, 284, 383, 435
Hin6I GCGC 2 cut(s) 542, 566
HinP1I GCGC 2 cut(s) 542, 566
HpaII CCGG 1 cut(s) 520
HphI GGTGA 1 cut(s) 380
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 3 cut(s) 15, 141, 549
Hpy188III TCNNGA 3 cut(s) 184, 396, 520
Hpy8I GTNNAC 1 cut(s) 97
HpyAV CCTTC 1 cut(s) 495
HpyCH4III ACNGT 2 cut(s) 147, 320
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 6 cut(s) 66, 77, 123, 305, 383, 446
HpyF10VI GCNNNNNNNGC 5 cut(s) 25, 77, 212, 242, 251
HpyF3I CTNAG 1 cut(s) 230
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 4 cut(s) 123, 284, 383, 435
HspAI GCGC 2 cut(s) 542, 566
Kpn2I TCCGGA 1 cut(s) 519
KspI CCGCGG 1 cut(s) 255
Kzo9I GATC 1 cut(s) 537
LpnPI CCDG 7 cut(s) 50, 76, 159, 202, 250, 478, 533
Lsp1109I GCAGC 7 cut(s) 58, 61, 89, 135, 317, 339, 430
LweI GCATC 4 cut(s) 6, 89, 328, 553
MaeI CTAG 3 cut(s) 20, 87, 203
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 2 cut(s) 127, 223
MalI GATC 1 cut(s) 539
MboI GATC 1 cut(s) 537
MboII GAAGA 2 cut(s) 75, 547
MluCI AATT 3 cut(s) 371, 414, 491
MnlI CCTC 7 cut(s) 166, 170, 227, 292, 319, 451, 516
MroI TCCGGA 1 cut(s) 519
MseI TTAA 3 cut(s) 48, 387, 449
MspA1I CMGCKG 2 cut(s) 71, 254
MspI CCGG 1 cut(s) 520
Mva1269I GAATGC 1 cut(s) 11
MvnI CGCG 2 cut(s) 254, 542
MwoI GCNNNNNNNGC 5 cut(s) 25, 77, 212, 242, 251
NdeII GATC 1 cut(s) 537
NheI GCTAGC 2 cut(s) 19, 202
NlaIII CATG 4 cut(s) 123, 284, 383, 435
NmuCI GTSAC 2 cut(s) 127, 223
PaeR7I CTCGAG 1 cut(s) 394
PaqCI CACCTGC 1 cut(s) 473
PcsI WCGNNNNNNNCGW 1 cut(s) 137
PctI GAATGC 1 cut(s) 11
PkrI GCNGC 7 cut(s) 73, 76, 79, 125, 307, 354, 445
PshAI GACNNNNGTC 1 cut(s) 515
PsrI GAACNNNNNNTAC 2 cut(s) 89, 121
PstI CTGCAG 1 cut(s) 79
PstNI CAGNNNCTG 1 cut(s) 74
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 1 cut(s) 107
RsaNI GTAC 1 cut(s) 106
SacII CCGCGG 1 cut(s) 255
SaqAI TTAA 3 cut(s) 48, 387, 449
SatI GCNGC 7 cut(s) 72, 75, 78, 124, 306, 353, 444
Sau3AI GATC 1 cut(s) 537
SfaNI GCATC 4 cut(s) 6, 89, 328, 553
SfcI CTRYAG 2 cut(s) 75, 294
Sfr274I CTCGAG 1 cut(s) 394
Sfr303I CCGCGG 1 cut(s) 255
SgrBI CCGCGG 1 cut(s) 255
SlaI CTCGAG 1 cut(s) 394
SmlI CTYRAG 1 cut(s) 394
SmoI CTYRAG 1 cut(s) 394
Sse9I AATT 3 cut(s) 371, 414, 491
SsiI CCGC 3 cut(s) 213, 252, 254
SspMI CTAG 3 cut(s) 20, 87, 203
StyI CCWWGG 1 cut(s) 561
TaaI ACNGT 2 cut(s) 147, 320
TaiI ACGT 1 cut(s) 225
TaqI TCGA 2 cut(s) 315, 395
TasI AATT 3 cut(s) 371, 414, 491
TatI WGTACW 1 cut(s) 105
Tru1I TTAA 3 cut(s) 48, 387, 449
Tru9I TTAA 3 cut(s) 48, 387, 449
TseFI GTSAC 2 cut(s) 127, 223
TseI GCWGC 7 cut(s) 71, 74, 77, 123, 305, 352, 443
Tsp45I GTSAC 2 cut(s) 127, 223
TspDTI ATGAA 2 cut(s) 269, 498
XcmI CCANNNNNNNNNTGG 1 cut(s) 370
XhoI CTCGAG 1 cut(s) 394
XspI CTAG 3 cut(s) 20, 87, 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.