RLG00000003626

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0017295
Physical Location & Seq
Reverse (-)
50918336 .. 50918929
594 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003626

Sequence Viewer

Length: 594 bp
ATGGAATGCCATCTTATGCTAGCTCTGCCTATTCTCCAGTTGCTTCTTTACCACATAAGCCCCTGCACAGCCTCCCCAGCATCTTATTGCAAAGCAAACACCGAGTACATAAAGACATCATGCAGCGTCACAATCTATCCGAAATTGTGCTACACCTCCCTCTCAATCTATGCAGGAAAAATCGAGACCAACCCGAAAGTGCTAGCTTATACCGCCCTCAATGTGACCCTTGCAGCCACCAAAGCAACTTCTGTGGTAATAAACAGGTGTTCAAGGGTTCATGGACTGAAGCCTATAGAGACTGCAGCTGCACTCGACTGTGTGGAGGAGGTTAGTGACGCTTTGGATGAGCTCAGGATGTCCATAGATGAATTGGGTCATGCAGTTAGAGGCTCGAGAAATTTCTCTTTTCAAATTAGTGATATTCAAACATGGGTTAGTGCTGCATTAACTGATGAGGACACCTGCATGGATGGCTTTGATGAGCTTAAATTGACTGGGAGGATGGAGACTGTTGTCCGGAGGAAAATACTGAAAATCGCGCATCTGACGAGCAATGCCTTGGCACTCATCAACAACTATGCCTCGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

198

Amino Acids

21.62

Weight (kDa)

6.2

Isoelectric Point (pI)

38.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 36 - 190 2.1e-36 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017295)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g00420
malus_domestica MD06G1064700.v1.1
prunus_persica Prupe.5G076800_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0202091
rosa_laevigata RLG00000003626
rosa_multiflora Rmu_sc0006592.1_g000014
rosa_roxburghii Rroxscaffold_3G00254880
rosa_rugosa Rorug07G0067900
rosa_samantha Rh7AG195400 Rh7BG196700 Rh7CG206500 Rh7DG202000
rosa_wichuraiana Rw7G017110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 473
Acc36I ACCTGC 1 cut(s) 473
AccII CGCG 1 cut(s) 542
AccIII TCCGGA 1 cut(s) 519
AciI CCGC 1 cut(s) 213
AcsI RAATTY 1 cut(s) 400
AcuI CTGAAG 1 cut(s) 308
AfaI GTAC 1 cut(s) 107
AgsI TTSAA 3 cut(s) 273, 413, 428
AluBI AGCT 5 cut(s) 23, 206, 308, 352, 487
AluI AGCT 5 cut(s) 23, 206, 308, 352, 487
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 3 cut(s) 179, 293, 503
Ama87I CYCGRG 1 cut(s) 394
Aor13HI TCCGGA 1 cut(s) 519
ApeKI GCWGC 5 cut(s) 123, 233, 305, 308, 443
ApoI RAATTY 1 cut(s) 400
AspLEI GCGC 1 cut(s) 544
AsuNHI GCTAGC 2 cut(s) 19, 202
AvaI CYCGRG 1 cut(s) 394
BanII GRGCYC 1 cut(s) 354
Bbv12I GWGCWC 1 cut(s) 354
BbvI GCAGC 5 cut(s) 135, 245, 295, 317, 430
BccI CCATC 3 cut(s) 18, 467, 499
BcoDI GTCTC 3 cut(s) 179, 293, 503
BfaI CTAG 2 cut(s) 20, 203
BfmI CTRYAG 2 cut(s) 294, 303
BfuAI ACCTGC 1 cut(s) 473
BisI GCNGC 5 cut(s) 124, 234, 306, 309, 444
BlsI GCNGC 5 cut(s) 125, 235, 307, 310, 445
BmeT110I CYCGRG 1 cut(s) 394
BmrI ACTGGG 1 cut(s) 507
BmsI GCATC 2 cut(s) 89, 553
BmtI GCTAGC 2 cut(s) 23, 206
BmuI ACTGGG 1 cut(s) 507
BoxI GACNNNNGTC 1 cut(s) 515
BpmI CTGGAG 1 cut(s) 20
Bpu10I CCTNAGC 1 cut(s) 353
BsaI GGTCTC 1 cut(s) 179
BsaJI CCNNGG 1 cut(s) 561
BsaWI WCCGGW 1 cut(s) 519
Bse1I ACTGG 2 cut(s) 37, 502
Bse3DI GCAATG 1 cut(s) 562
BseAI TCCGGA 1 cut(s) 519
BseDI CCNNGG 1 cut(s) 561
BseGI GGATG 4 cut(s) 352, 363, 478, 510
BseMI GCAATG 1 cut(s) 562
BseMII CTCAG 1 cut(s) 367
BseNI ACTGG 2 cut(s) 37, 502
BseRI GAGGAG 1 cut(s) 341
BseXI GCAGC 5 cut(s) 135, 245, 295, 317, 430
BseYI CCCAGC 1 cut(s) 76
BsgI GTGCAG 2 cut(s) 49, 294
Bsh1236I CGCG 1 cut(s) 542
BsiHKAI GWGCWC 1 cut(s) 354
BsiHKCI CYCGRG 1 cut(s) 394
BsiSI CCGG 1 cut(s) 520
BsmAI GTCTC 3 cut(s) 179, 293, 503
BsmI GAATGC 1 cut(s) 11
Bso31I GGTCTC 1 cut(s) 179
BsoBI CYCGRG 1 cut(s) 394
Bsp1286I GDGCHC 1 cut(s) 354
Bsp13I TCCGGA 1 cut(s) 519
BspACI CCGC 1 cut(s) 213
BspCNI CTCAG 1 cut(s) 366
BspEI TCCGGA 1 cut(s) 519
BspFNI CGCG 1 cut(s) 542
BspMAI CTGCAG 1 cut(s) 307
BspMI ACCTGC 1 cut(s) 473
BspOI GCTAGC 2 cut(s) 23, 206
BspTNI GGTCTC 1 cut(s) 179
BsrDI GCAATG 1 cut(s) 562
BsrI ACTGG 2 cut(s) 37, 502
BssECI CCNNGG 1 cut(s) 561
BssT1I CCWWGG 1 cut(s) 561
Bst4CI ACNGT 2 cut(s) 320, 514
BstC8I GCNNGC 2 cut(s) 21, 204
BstDEI CTNAG 1 cut(s) 353
BstF5I GGATG 4 cut(s) 352, 363, 478, 510
BstFNI CGCG 1 cut(s) 542
BstHHI GCGC 1 cut(s) 544
BstMAI GTCTC 3 cut(s) 179, 293, 503
BstMWI GCNNNNNNNGC 5 cut(s) 25, 77, 212, 242, 474
BstPAI GACNNNNGTC 1 cut(s) 515
BstSFI CTRYAG 2 cut(s) 294, 303
BstUI CGCG 1 cut(s) 542
BstV1I GCAGC 5 cut(s) 135, 245, 295, 317, 430
BtsCI GGATG 4 cut(s) 352, 363, 478, 510
BveI ACCTGC 1 cut(s) 473
Cac8I GCNNGC 2 cut(s) 21, 204
CfoI GCGC 1 cut(s) 544
CseI GACGC 2 cut(s) 115, 347
Csp6I GTAC 1 cut(s) 106
CspCI CAANNNNNGTGG 2 cut(s) 234, 269
CviAII CATG 5 cut(s) 120, 281, 380, 432, 469
CviQI GTAC 1 cut(s) 106
DdeI CTNAG 1 cut(s) 353
Ecl136II GAGCTC 1 cut(s) 352
Eco130I CCWWGG 1 cut(s) 561
Eco24I GRGCYC 1 cut(s) 354
Eco31I GGTCTC 1 cut(s) 179
Eco53kI GAGCTC 1 cut(s) 352
Eco57I CTGAAG 1 cut(s) 308
Eco88I CYCGRG 1 cut(s) 394
EcoICRI GAGCTC 1 cut(s) 352
EcoT14I CCWWGG 1 cut(s) 561
EcoT38I GRGCYC 1 cut(s) 354
ErhI CCWWGG 1 cut(s) 561
FaeI CATG 5 cut(s) 123, 284, 383, 435, 472
FatI CATG 5 cut(s) 119, 280, 379, 431, 468
Fnu4HI GCNGC 5 cut(s) 124, 234, 306, 309, 444
FokI GGATG 4 cut(s) 359, 370, 485, 517
FriOI GRGCYC 1 cut(s) 354
Fsp4HI GCNGC 5 cut(s) 124, 234, 306, 309, 444
FspBI CTAG 2 cut(s) 20, 203
GlaI GCGC 1 cut(s) 543
GluI GCNGC 5 cut(s) 124, 234, 306, 309, 444
GsaI CCCAGC 1 cut(s) 80
GsuI CTGGAG 1 cut(s) 20
HapII CCGG 1 cut(s) 520
HgaI GACGC 2 cut(s) 115, 347
HhaI GCGC 1 cut(s) 544
Hin1II CATG 5 cut(s) 123, 284, 383, 435, 472
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HpaII CCGG 1 cut(s) 520
Hpy188I TCNGA 2 cut(s) 141, 549
Hpy188III TCNNGA 4 cut(s) 184, 355, 396, 520
HpyCH4III ACNGT 2 cut(s) 320, 514
HpyF10VI GCNNNNNNNGC 5 cut(s) 25, 77, 212, 242, 474
HpyF3I CTNAG 1 cut(s) 353
Hsp92II CATG 5 cut(s) 123, 284, 383, 435, 472
HspAI GCGC 1 cut(s) 542
Kpn2I TCCGGA 1 cut(s) 519
LpnPI CCDG 9 cut(s) 50, 76, 90, 159, 250, 340, 478, 483, 533
Lsp1109I GCAGC 5 cut(s) 135, 245, 295, 317, 430
LweI GCATC 2 cut(s) 89, 553
MaeI CTAG 2 cut(s) 20, 203
MaeIII GTNAC 3 cut(s) 127, 223, 335
MhlI GDGCHC 1 cut(s) 354
MluCI AATT 5 cut(s) 143, 371, 400, 414, 491
MroI TCCGGA 1 cut(s) 519
MseI TTAA 2 cut(s) 449, 489
MslI CAYNNNNRTG 1 cut(s) 467
MspA1I CMGCKG 1 cut(s) 308
MspI CCGG 1 cut(s) 520
Mva1269I GAATGC 1 cut(s) 11
MvnI CGCG 1 cut(s) 542
MwoI GCNNNNNNNGC 5 cut(s) 25, 77, 212, 242, 474
NheI GCTAGC 2 cut(s) 19, 202
NlaIII CATG 5 cut(s) 123, 284, 383, 435, 472
NmuCI GTSAC 3 cut(s) 127, 223, 335
PaeR7I CTCGAG 1 cut(s) 394
PaqCI CACCTGC 1 cut(s) 473
PctI GAATGC 1 cut(s) 11
PkrI GCNGC 5 cut(s) 125, 235, 307, 310, 445
PshAI GACNNNNGTC 1 cut(s) 515
Psp124BI GAGCTC 1 cut(s) 354
PspFI CCCAGC 1 cut(s) 76
PstI CTGCAG 1 cut(s) 307
PvuII CAGCTG 1 cut(s) 308
RsaI GTAC 1 cut(s) 107
RsaNI GTAC 1 cut(s) 106
RseI CAYNNNNRTG 1 cut(s) 467
SacI GAGCTC 1 cut(s) 354
SaqAI TTAA 2 cut(s) 449, 489
SatI GCNGC 5 cut(s) 124, 234, 306, 309, 444
SduI GDGCHC 1 cut(s) 354
SetI ASST 9 cut(s) 25, 158, 208, 269, 310, 333, 354, 467, 489
SfaNI GCATC 2 cut(s) 89, 553
SfcI CTRYAG 2 cut(s) 294, 303
Sfr274I CTCGAG 1 cut(s) 394
SlaI CTCGAG 1 cut(s) 394
SmiMI CAYNNNNRTG 1 cut(s) 467
SmlI CTYRAG 1 cut(s) 394
SmoI CTYRAG 1 cut(s) 394
Sse9I AATT 5 cut(s) 143, 371, 400, 414, 491
SsiI CCGC 1 cut(s) 213
SspMI CTAG 2 cut(s) 20, 203
SstI GAGCTC 1 cut(s) 354
StyI CCWWGG 1 cut(s) 561
TaaI ACNGT 2 cut(s) 320, 514
TaqI TCGA 4 cut(s) 183, 315, 395, 587
TasI AATT 5 cut(s) 143, 371, 400, 414, 491
TatI WGTACW 1 cut(s) 105
Tru1I TTAA 2 cut(s) 449, 489
Tru9I TTAA 2 cut(s) 449, 489
TseFI GTSAC 3 cut(s) 127, 223, 335
TseI GCWGC 5 cut(s) 123, 233, 305, 308, 443
Tsp45I GTSAC 3 cut(s) 127, 223, 335
TspDTI ATGAA 2 cut(s) 269, 384
XapI RAATTY 1 cut(s) 400
XcmI CCANNNNNNNNNTGG 1 cut(s) 370
XhoI CTCGAG 1 cut(s) 394
XspI CTAG 2 cut(s) 20, 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.