Rh7AG195400

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0017295
Physical Location & Seq
Forward (+)
17530752 .. 17531330
579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG195400.1

Sequence Viewer

Length: 579 bp
ATGCTAGCTCTGCCTATTCTCCAGTTGCTTCTTTACCACATAAGCCCCTGCACAGCCGCCCCAGCATCTTCTTGCAAAGCAAACATCGAGTACATAAAGACATCATGCAGCGTCACAATCTATCCGAAATTGTGCTACACCTCCCTCTCAATCTATGCAGGAAAAATCAAGACCAACCCGAAAGTGCTAGCTTATACCGCCCTCAACGTGACCCTTGCAGCCACCAAAGCAACTTCTGTGGTAATAAACAGGTGTTCAAGGGTTCATGGACTGAAGCCTATAGAGACTGCAGCTGCACTCGACTGTGTGGAGGAGGTTAGTGACGCTCTGGATGAGCTCCGGATGTCCATAGATGAATTGGGTCATGCAGTTAGAGGCTCGAGAAATTTCTCGTTTCAAATTAGCGATATTCAAACATGGGTTAGTGCTGCATTAACTGATGAGGACACCTGCATGGATGGCTTTGATGAGCATAAATTGACTGGGAGGATGGAGACTGTTGTCCGGAGGAAAATACTGAAAATCGCGCATCTGACGAGCAATGCCTTGGCACTCGTCAACAACTATGCCTCGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

192

Amino Acids

20.94

Weight (kDa)

7.59

Isoelectric Point (pI)

39.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 31 - 185 1.2e-36 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017295)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g00420
malus_domestica MD06G1064700.v1.1
prunus_persica Prupe.5G076800_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0202091
rosa_laevigata RLG00000003626
rosa_multiflora Rmu_sc0006592.1_g000014
rosa_roxburghii Rroxscaffold_3G00254880
rosa_rugosa Rorug07G0067900
rosa_samantha Rh7AG195400 Rh7BG196700 Rh7CG206500 Rh7DG202000
rosa_wichuraiana Rw7G017110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 458
Acc36I ACCTGC 1 cut(s) 458
AccII CGCG 1 cut(s) 527
AccIII TCCGGA 2 cut(s) 339, 504
AciI CCGC 2 cut(s) 57, 198
AcsI RAATTY 1 cut(s) 385
AcuI CTGAAG 1 cut(s) 293
AfaI GTAC 1 cut(s) 92
AgsI TTSAA 3 cut(s) 258, 398, 413
AluBI AGCT 4 cut(s) 8, 191, 293, 337
AluI AGCT 4 cut(s) 8, 191, 293, 337
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 2 cut(s) 278, 488
Ama87I CYCGRG 1 cut(s) 379
Aor13HI TCCGGA 2 cut(s) 339, 504
ApeKI GCWGC 5 cut(s) 108, 218, 290, 293, 428
ApoI RAATTY 1 cut(s) 385
AspLEI GCGC 1 cut(s) 529
AsuNHI GCTAGC 2 cut(s) 4, 187
AvaI CYCGRG 1 cut(s) 379
BanII GRGCYC 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 339
BbvI GCAGC 5 cut(s) 120, 230, 280, 302, 415
BccI CCATC 2 cut(s) 452, 484
BcoDI GTCTC 2 cut(s) 278, 488
BfaI CTAG 2 cut(s) 5, 188
BfmI CTRYAG 2 cut(s) 279, 288
BfuAI ACCTGC 1 cut(s) 458
BisI GCNGC 6 cut(s) 57, 109, 219, 291, 294, 429
BlsI GCNGC 6 cut(s) 58, 110, 220, 292, 295, 430
BmeT110I CYCGRG 1 cut(s) 379
BmrI ACTGGG 1 cut(s) 492
BmsI GCATC 2 cut(s) 74, 538
BmtI GCTAGC 2 cut(s) 8, 191
BmuI ACTGGG 1 cut(s) 492
BoxI GACNNNNGTC 1 cut(s) 500
BpmI CTGGAG 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 546
BsaWI WCCGGW 2 cut(s) 339, 504
Bse1I ACTGG 2 cut(s) 22, 487
Bse3DI GCAATG 1 cut(s) 547
BseAI TCCGGA 2 cut(s) 339, 504
BseDI CCNNGG 1 cut(s) 546
BseGI GGATG 4 cut(s) 337, 348, 463, 495
BseMI GCAATG 1 cut(s) 547
BseNI ACTGG 2 cut(s) 22, 487
BseRI GAGGAG 1 cut(s) 326
BseXI GCAGC 5 cut(s) 120, 230, 280, 302, 415
BseYI CCCAGC 1 cut(s) 61
BsgI GTGCAG 2 cut(s) 34, 279
Bsh1236I CGCG 1 cut(s) 527
BsiHKAI GWGCWC 1 cut(s) 339
BsiHKCI CYCGRG 1 cut(s) 379
BsiSI CCGG 2 cut(s) 340, 505
BsmAI GTCTC 2 cut(s) 278, 488
BsoBI CYCGRG 1 cut(s) 379
Bsp1286I GDGCHC 1 cut(s) 339
Bsp13I TCCGGA 2 cut(s) 339, 504
BspACI CCGC 2 cut(s) 57, 198
BspEI TCCGGA 2 cut(s) 339, 504
BspFNI CGCG 1 cut(s) 527
BspMAI CTGCAG 1 cut(s) 292
BspMI ACCTGC 1 cut(s) 458
BspOI GCTAGC 2 cut(s) 8, 191
BsrDI GCAATG 1 cut(s) 547
BsrI ACTGG 2 cut(s) 22, 487
BssECI CCNNGG 1 cut(s) 546
BssT1I CCWWGG 1 cut(s) 546
Bst4CI ACNGT 2 cut(s) 305, 499
BstC8I GCNNGC 2 cut(s) 6, 189
BstF5I GGATG 4 cut(s) 337, 348, 463, 495
BstFNI CGCG 1 cut(s) 527
BstHHI GCGC 1 cut(s) 529
BstMAI GTCTC 2 cut(s) 278, 488
BstMWI GCNNNNNNNGC 5 cut(s) 10, 62, 197, 227, 459
BstPAI GACNNNNGTC 1 cut(s) 500
BstSFI CTRYAG 2 cut(s) 279, 288
BstUI CGCG 1 cut(s) 527
BstV1I GCAGC 5 cut(s) 120, 230, 280, 302, 415
BtsCI GGATG 4 cut(s) 337, 348, 463, 495
BveI ACCTGC 1 cut(s) 458
Cac8I GCNNGC 2 cut(s) 6, 189
CfoI GCGC 1 cut(s) 529
CseI GACGC 2 cut(s) 100, 332
Csp6I GTAC 1 cut(s) 91
CspCI CAANNNNNGTGG 2 cut(s) 219, 254
CviAII CATG 5 cut(s) 105, 266, 365, 417, 454
CviQI GTAC 1 cut(s) 91
Ecl136II GAGCTC 1 cut(s) 337
Eco130I CCWWGG 1 cut(s) 546
Eco24I GRGCYC 1 cut(s) 339
Eco53kI GAGCTC 1 cut(s) 337
Eco57I CTGAAG 1 cut(s) 293
Eco88I CYCGRG 1 cut(s) 379
EcoICRI GAGCTC 1 cut(s) 337
EcoT14I CCWWGG 1 cut(s) 546
EcoT38I GRGCYC 1 cut(s) 339
ErhI CCWWGG 1 cut(s) 546
FaeI CATG 5 cut(s) 108, 269, 368, 420, 457
FatI CATG 5 cut(s) 104, 265, 364, 416, 453
Fnu4HI GCNGC 6 cut(s) 57, 109, 219, 291, 294, 429
FokI GGATG 4 cut(s) 344, 355, 470, 502
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 6 cut(s) 57, 109, 219, 291, 294, 429
FspBI CTAG 2 cut(s) 5, 188
GlaI GCGC 1 cut(s) 528
GluI GCNGC 6 cut(s) 57, 109, 219, 291, 294, 429
GsaI CCCAGC 1 cut(s) 65
GsuI CTGGAG 1 cut(s) 5
HapII CCGG 2 cut(s) 340, 505
HgaI GACGC 2 cut(s) 100, 332
HhaI GCGC 1 cut(s) 529
Hin1II CATG 5 cut(s) 108, 269, 368, 420, 457
Hin6I GCGC 1 cut(s) 527
HinP1I GCGC 1 cut(s) 527
HincII GTYRAC 1 cut(s) 559
HindII GTYRAC 1 cut(s) 559
HpaII CCGG 2 cut(s) 340, 505
Hpy166II GTNNAC 1 cut(s) 559
Hpy188I TCNGA 2 cut(s) 126, 534
Hpy188III TCNNGA 5 cut(s) 169, 329, 340, 381, 505
Hpy8I GTNNAC 1 cut(s) 559
HpyCH4III ACNGT 2 cut(s) 305, 499
HpyCH4IV ACGT 1 cut(s) 207
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 62, 197, 227, 459
HpySE526I ACGT 1 cut(s) 207
Hsp92II CATG 5 cut(s) 108, 269, 368, 420, 457
HspAI GCGC 1 cut(s) 527
Kpn2I TCCGGA 2 cut(s) 339, 504
LmnI GCTCC 1 cut(s) 342
Lsp1109I GCAGC 5 cut(s) 120, 230, 280, 302, 415
LweI GCATC 2 cut(s) 74, 538
MaeI CTAG 2 cut(s) 5, 188
MaeII ACGT 1 cut(s) 207
MaeIII GTNAC 3 cut(s) 112, 208, 320
MboII GAAGA 1 cut(s) 60
MhlI GDGCHC 1 cut(s) 339
MluCI AATT 5 cut(s) 128, 356, 385, 399, 476
MnlI CCTC 9 cut(s) 151, 155, 212, 304, 307, 368, 436, 480, 501
MroI TCCGGA 2 cut(s) 339, 504
MseI TTAA 1 cut(s) 434
MslI CAYNNNNRTG 1 cut(s) 452
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 2 cut(s) 340, 505
MvnI CGCG 1 cut(s) 527
MwoI GCNNNNNNNGC 5 cut(s) 10, 62, 197, 227, 459
NheI GCTAGC 2 cut(s) 4, 187
NlaIII CATG 5 cut(s) 108, 269, 368, 420, 457
NmuCI GTSAC 3 cut(s) 112, 208, 320
PaeR7I CTCGAG 1 cut(s) 379
PaqCI CACCTGC 1 cut(s) 458
PkrI GCNGC 6 cut(s) 58, 110, 220, 292, 295, 430
PshAI GACNNNNGTC 1 cut(s) 500
Psp124BI GAGCTC 1 cut(s) 339
PspFI CCCAGC 1 cut(s) 61
PstI CTGCAG 1 cut(s) 292
PvuII CAGCTG 1 cut(s) 293
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
RseI CAYNNNNRTG 1 cut(s) 452
SacI GAGCTC 1 cut(s) 339
SaqAI TTAA 1 cut(s) 434
SatI GCNGC 6 cut(s) 57, 109, 219, 291, 294, 429
SduI GDGCHC 1 cut(s) 339
SetI ASST 9 cut(s) 10, 143, 193, 210, 254, 295, 318, 339, 452
SfaNI GCATC 2 cut(s) 74, 538
SfcI CTRYAG 2 cut(s) 279, 288
Sfr274I CTCGAG 1 cut(s) 379
SlaI CTCGAG 1 cut(s) 379
SmiMI CAYNNNNRTG 1 cut(s) 452
SmlI CTYRAG 1 cut(s) 379
SmoI CTYRAG 1 cut(s) 379
Sse9I AATT 5 cut(s) 128, 356, 385, 399, 476
SsiI CCGC 2 cut(s) 57, 198
SspMI CTAG 2 cut(s) 5, 188
SstI GAGCTC 1 cut(s) 339
StyI CCWWGG 1 cut(s) 546
TaaI ACNGT 2 cut(s) 305, 499
TaiI ACGT 1 cut(s) 210
TaqI TCGA 4 cut(s) 87, 300, 380, 572
TasI AATT 5 cut(s) 128, 356, 385, 399, 476
TatI WGTACW 1 cut(s) 90
TauI GCSGC 1 cut(s) 59
Tru1I TTAA 1 cut(s) 434
Tru9I TTAA 1 cut(s) 434
TseFI GTSAC 3 cut(s) 112, 208, 320
TseI GCWGC 5 cut(s) 108, 218, 290, 293, 428
Tsp45I GTSAC 3 cut(s) 112, 208, 320
TspDTI ATGAA 2 cut(s) 254, 369
XapI RAATTY 1 cut(s) 385
XcmI CCANNNNNNNNNTGG 1 cut(s) 355
XhoI CTCGAG 1 cut(s) 379
XspI CTAG 2 cut(s) 5, 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.