Rh7BG196700

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0017295
Physical Location & Seq
Forward (+)
16127381 .. 16128708
1328 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG196700.1

Sequence Viewer

Length: 669 bp
ATGCCTTCCTCTATAAATTCATCAATCTCTCAAAAGGAAGAAAGGAAGTCTTCAAGAGAGGAGAGAGAGAGAGAGATGGAATGCCATCTTATGCTAGCTCTGCCTATTCTCCAGTTGCTTCTTTACCACATAAGCCCCTGCACAGCCTCCCCAGCATCTTCTTGCAAAGCAAACACCGAGTACATAAAGACATCATGCAGCGTCACAATCTATCCGAAATTGTGCTACACCTCCCTCTCAATCTATGCAGAAAAAATCAAGACCAACCCGAAAGTGCTAGCTTATACCGCCCTCAATGTGACCCTTGCAGCCACCAAAGCAAGTTCTGTGGTAATAAACAGGTGTTCAAGGGTTCATGGACTGAAGCCTATAGAGACTGCAGCTGCACTCGACTGTGTGGAGGAGGTTAGTGACGCTTTGGATGAGCTCCGGATGTCCATAGATGAATTGGGTCATGCAGTTAGAGGCTCGAGAAATTTCTCTTTTCAAATTAGTGATATTCAAACATGGGTTAGTGCTGCATTAACCGATGAGGACACCTGCATGGATGGCTTTGATGAGCATAAATCGACTGGGAGGATGGAGACTGTTGTCCGGAGGGAAATACTGAAAATCACGCATCTGACGAGCAATGCCTTGGCACTCATCAACAACTATGCCTCGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

222

Amino Acids

24.59

Weight (kDa)

6.09

Isoelectric Point (pI)

49.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 61 - 215 6.9e-36 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017295)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g00420
malus_domestica MD06G1064700.v1.1
prunus_persica Prupe.5G076800_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0202091
rosa_laevigata RLG00000003626
rosa_multiflora Rmu_sc0006592.1_g000014
rosa_roxburghii Rroxscaffold_3G00254880
rosa_rugosa Rorug07G0067900
rosa_samantha Rh7AG195400 Rh7BG196700 Rh7CG206500 Rh7DG202000
rosa_wichuraiana Rw7G017110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 548
Acc36I ACCTGC 1 cut(s) 548
AccIII TCCGGA 2 cut(s) 429, 594
AciI CCGC 1 cut(s) 288
AcsI RAATTY 2 cut(s) 16, 475
AcuI CTGAAG 1 cut(s) 383
AfaI GTAC 1 cut(s) 182
AgsI TTSAA 4 cut(s) 54, 348, 488, 503
AluBI AGCT 4 cut(s) 98, 281, 383, 427
AluI AGCT 4 cut(s) 98, 281, 383, 427
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 2 cut(s) 368, 578
Ama87I CYCGRG 1 cut(s) 469
Aor13HI TCCGGA 2 cut(s) 429, 594
ApeKI GCWGC 5 cut(s) 198, 308, 380, 383, 518
ApoI RAATTY 2 cut(s) 16, 475
AsuNHI GCTAGC 2 cut(s) 94, 277
AvaI CYCGRG 1 cut(s) 469
BanII GRGCYC 1 cut(s) 429
BbsI GAAGAC 1 cut(s) 42
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 5 cut(s) 210, 320, 370, 392, 505
BccI CCATC 4 cut(s) 70, 93, 542, 574
BcoDI GTCTC 2 cut(s) 368, 578
BfaI CTAG 2 cut(s) 95, 278
BfmI CTRYAG 2 cut(s) 369, 378
BfuAI ACCTGC 1 cut(s) 548
BisI GCNGC 5 cut(s) 199, 309, 381, 384, 519
BlsI GCNGC 5 cut(s) 200, 310, 382, 385, 520
BmeT110I CYCGRG 1 cut(s) 469
BmrI ACTGGG 1 cut(s) 582
BmsI GCATC 2 cut(s) 164, 628
BmtI GCTAGC 2 cut(s) 98, 281
BmuI ACTGGG 1 cut(s) 582
BoxI GACNNNNGTC 1 cut(s) 590
BpiI GAAGAC 1 cut(s) 42
BpmI CTGGAG 1 cut(s) 95
BsaJI CCNNGG 1 cut(s) 636
BsaWI WCCGGW 2 cut(s) 429, 594
Bse1I ACTGG 2 cut(s) 112, 577
Bse3DI GCAATG 1 cut(s) 637
BseAI TCCGGA 2 cut(s) 429, 594
BseDI CCNNGG 1 cut(s) 636
BseGI GGATG 4 cut(s) 427, 438, 553, 585
BseMI GCAATG 1 cut(s) 637
BseNI ACTGG 2 cut(s) 112, 577
BseRI GAGGAG 2 cut(s) 74, 416
BseXI GCAGC 5 cut(s) 210, 320, 370, 392, 505
BseYI CCCAGC 1 cut(s) 151
BsgI GTGCAG 2 cut(s) 124, 369
BsiHKAI GWGCWC 1 cut(s) 429
BsiHKCI CYCGRG 1 cut(s) 469
BsiSI CCGG 2 cut(s) 430, 595
BsmAI GTCTC 2 cut(s) 368, 578
BsmI GAATGC 1 cut(s) 86
BsoBI CYCGRG 1 cut(s) 469
Bsp1286I GDGCHC 1 cut(s) 429
Bsp13I TCCGGA 2 cut(s) 429, 594
BspACI CCGC 1 cut(s) 288
BspEI TCCGGA 2 cut(s) 429, 594
BspMAI CTGCAG 1 cut(s) 382
BspMI ACCTGC 1 cut(s) 548
BspOI GCTAGC 2 cut(s) 98, 281
BsrDI GCAATG 1 cut(s) 637
BsrI ACTGG 2 cut(s) 112, 577
BssECI CCNNGG 1 cut(s) 636
BssT1I CCWWGG 1 cut(s) 636
Bst4CI ACNGT 2 cut(s) 395, 589
BstC8I GCNNGC 2 cut(s) 96, 279
BstF5I GGATG 4 cut(s) 427, 438, 553, 585
BstMAI GTCTC 2 cut(s) 368, 578
BstMWI GCNNNNNNNGC 5 cut(s) 100, 152, 287, 317, 549
BstPAI GACNNNNGTC 1 cut(s) 590
BstSFI CTRYAG 2 cut(s) 369, 378
BstV1I GCAGC 5 cut(s) 210, 320, 370, 392, 505
BstV2I GAAGAC 1 cut(s) 42
BtsCI GGATG 4 cut(s) 427, 438, 553, 585
BveI ACCTGC 1 cut(s) 548
Cac8I GCNNGC 2 cut(s) 96, 279
CseI GACGC 2 cut(s) 190, 422
Csp6I GTAC 1 cut(s) 181
CspCI CAANNNNNGTGG 2 cut(s) 309, 344
CviAII CATG 5 cut(s) 195, 356, 455, 507, 544
CviQI GTAC 1 cut(s) 181
Ecl136II GAGCTC 1 cut(s) 427
Eco130I CCWWGG 1 cut(s) 636
Eco24I GRGCYC 1 cut(s) 429
Eco53kI GAGCTC 1 cut(s) 427
Eco57I CTGAAG 1 cut(s) 383
Eco88I CYCGRG 1 cut(s) 469
EcoICRI GAGCTC 1 cut(s) 427
EcoT14I CCWWGG 1 cut(s) 636
EcoT38I GRGCYC 1 cut(s) 429
ErhI CCWWGG 1 cut(s) 636
FaeI CATG 5 cut(s) 198, 359, 458, 510, 547
FalI AAGNNNNNCTT 2 cut(s) 34, 66
FatI CATG 5 cut(s) 194, 355, 454, 506, 543
Fnu4HI GCNGC 5 cut(s) 199, 309, 381, 384, 519
FokI GGATG 4 cut(s) 434, 445, 560, 592
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 5 cut(s) 199, 309, 381, 384, 519
FspBI CTAG 2 cut(s) 95, 278
GluI GCNGC 5 cut(s) 199, 309, 381, 384, 519
GsaI CCCAGC 1 cut(s) 155
GsuI CTGGAG 1 cut(s) 95
HapII CCGG 2 cut(s) 430, 595
HgaI GACGC 2 cut(s) 190, 422
Hin1II CATG 5 cut(s) 198, 359, 458, 510, 547
HpaII CCGG 2 cut(s) 430, 595
Hpy188I TCNGA 2 cut(s) 216, 624
Hpy188III TCNNGA 5 cut(s) 54, 259, 430, 471, 595
HpyAV CCTTC 1 cut(s) 15
HpyCH4III ACNGT 2 cut(s) 395, 589
HpyF10VI GCNNNNNNNGC 5 cut(s) 100, 152, 287, 317, 549
Hsp92II CATG 5 cut(s) 198, 359, 458, 510, 547
Kpn2I TCCGGA 2 cut(s) 429, 594
LmnI GCTCC 1 cut(s) 432
LpnPI CCDG 8 cut(s) 125, 151, 165, 325, 443, 553, 558, 608
Lsp1109I GCAGC 5 cut(s) 210, 320, 370, 392, 505
LweI GCATC 2 cut(s) 164, 628
MaeI CTAG 2 cut(s) 95, 278
MaeIII GTNAC 3 cut(s) 202, 298, 410
MboII GAAGA 3 cut(s) 42, 50, 150
MhlI GDGCHC 1 cut(s) 429
MluCI AATT 5 cut(s) 16, 218, 446, 475, 489
MroI TCCGGA 2 cut(s) 429, 594
MseI TTAA 1 cut(s) 524
MslI CAYNNNNRTG 1 cut(s) 542
MspA1I CMGCKG 1 cut(s) 383
MspI CCGG 2 cut(s) 430, 595
Mva1269I GAATGC 1 cut(s) 86
MwoI GCNNNNNNNGC 5 cut(s) 100, 152, 287, 317, 549
NheI GCTAGC 2 cut(s) 94, 277
NlaIII CATG 5 cut(s) 198, 359, 458, 510, 547
NmuCI GTSAC 3 cut(s) 202, 298, 410
PaeR7I CTCGAG 1 cut(s) 469
PaqCI CACCTGC 1 cut(s) 548
PcsI WCGNNNNNNNCGW 1 cut(s) 623
PctI GAATGC 1 cut(s) 86
PkrI GCNGC 5 cut(s) 200, 310, 382, 385, 520
PshAI GACNNNNGTC 1 cut(s) 590
Psp124BI GAGCTC 1 cut(s) 429
PspFI CCCAGC 1 cut(s) 151
PstI CTGCAG 1 cut(s) 382
PvuII CAGCTG 1 cut(s) 383
RsaI GTAC 1 cut(s) 182
RsaNI GTAC 1 cut(s) 181
RseI CAYNNNNRTG 1 cut(s) 542
SacI GAGCTC 1 cut(s) 429
SaqAI TTAA 1 cut(s) 524
SatI GCNGC 5 cut(s) 199, 309, 381, 384, 519
SduI GDGCHC 1 cut(s) 429
SetI ASST 8 cut(s) 100, 233, 283, 344, 385, 408, 429, 542
SfaNI GCATC 2 cut(s) 164, 628
SfcI CTRYAG 2 cut(s) 369, 378
Sfr274I CTCGAG 1 cut(s) 469
SlaI CTCGAG 1 cut(s) 469
SmiMI CAYNNNNRTG 1 cut(s) 542
SmlI CTYRAG 1 cut(s) 469
SmoI CTYRAG 1 cut(s) 469
Sse9I AATT 5 cut(s) 16, 218, 446, 475, 489
SsiI CCGC 1 cut(s) 288
SspMI CTAG 2 cut(s) 95, 278
SstI GAGCTC 1 cut(s) 429
StyI CCWWGG 1 cut(s) 636
TaaI ACNGT 2 cut(s) 395, 589
TaqI TCGA 4 cut(s) 390, 470, 569, 662
TasI AATT 5 cut(s) 16, 218, 446, 475, 489
TatI WGTACW 1 cut(s) 180
Tru1I TTAA 1 cut(s) 524
Tru9I TTAA 1 cut(s) 524
TseFI GTSAC 3 cut(s) 202, 298, 410
TseI GCWGC 5 cut(s) 198, 308, 380, 383, 518
Tsp45I GTSAC 3 cut(s) 202, 298, 410
TspDTI ATGAA 3 cut(s) 9, 344, 459
XapI RAATTY 2 cut(s) 16, 475
XcmI CCANNNNNNNNNTGG 1 cut(s) 445
XhoI CTCGAG 1 cut(s) 469
XspI CTAG 2 cut(s) 95, 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.