MD06G1064700.v1.1

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0017295
Physical Location & Seq
Forward (+)
13335924 .. 13336526
603 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1064700.v1.1.491

Sequence Viewer

Length: 603 bp
ATGGAAGGCAATCTTACTCCCTTTGTACTATTATTGGCTCTGCTTACTCTCCAATTATGCAGTCCTGATGCATCTCCTAGCAGATCCAACACTCAGTACATAAGAACATCGTGCAGCGTCACAAACTATCCGAGGCTGTGCTACAACTCGCTCTCCATCTACGCCGGAAAAATCAAGACCAACCCGAAAGCACTAGCACACACTGCTCTTAACGTGACCATTGCTGCCACCCAAGAAACATCTGTGATCATGAGAAGGCTGTCCAAAATCCATGGCTTGAAACCCATAGAGTCTGCAGCAGCGCTTGACTGCATGGAGGAGATTGGTGATGCTGTTGACGAGCTCCAAAACTCGATAGATGAATTGAGTCATGTGATTAGGGGATCAAATTTCTGGCTTCAAATGAGTGATATCCAAACATGGGTTAGTGTTGCATTGACAGATGAGGACACCTGCATGGATGGATTTGATGAACATAATATGAGTGGAAGGGTGGAGAATCTTGTCCGTAGGTACATAGTGAATGTTGCGCATCTTACTTGCAATGCTTTGGCGCTAATCAACAGCTACGCCTCGGCCTCGACTGAAGCGTTTTTGCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

201

Amino Acids

21.98

Weight (kDa)

5.14

Isoelectric Point (pI)

40.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 33 - 186 1.5e-36 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017295)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g00420
malus_domestica MD06G1064700.v1.1
prunus_persica Prupe.5G076800_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0202091
rosa_laevigata RLG00000003626
rosa_multiflora Rmu_sc0006592.1_g000014
rosa_roxburghii Rroxscaffold_3G00254880
rosa_rugosa Rorug07G0067900
rosa_samantha Rh7AG195400 Rh7BG196700 Rh7CG206500 Rh7DG202000
rosa_wichuraiana Rw7G017110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 461
Acc16I TGCGCA 1 cut(s) 531
Acc36I ACCTGC 1 cut(s) 461
AclWI GGATC 2 cut(s) 78, 391
AcsI RAATTY 1 cut(s) 388
AfaI GTAC 3 cut(s) 27, 98, 515
AfeI AGCGCT 1 cut(s) 303
AfiI CCNNNNNNNGG 1 cut(s) 421
AgsI TTSAA 2 cut(s) 280, 401
AluBI AGCT 2 cut(s) 343, 567
AluI AGCT 2 cut(s) 343, 567
Alw21I GWGCWC 1 cut(s) 345
AlwI GGATC 2 cut(s) 78, 391
Aor51HI AGCGCT 1 cut(s) 303
AoxI GGCC 1 cut(s) 576
ApeKI GCWGC 4 cut(s) 114, 224, 296, 299
ApoI RAATTY 1 cut(s) 388
AspLEI GCGC 3 cut(s) 304, 532, 556
AsuHPI GGTGA 1 cut(s) 338
BanII GRGCYC 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 345
BbvI GCAGC 4 cut(s) 126, 211, 308, 311
BccI CCATC 2 cut(s) 164, 455
BceAI ACGGC 1 cut(s) 583
BclI TGATCA 1 cut(s) 246
BfaI CTAG 2 cut(s) 78, 194
BfmI CTRYAG 1 cut(s) 294
BfoI RGCGCY 2 cut(s) 305, 557
BfuAI ACCTGC 1 cut(s) 461
BisI GCNGC 4 cut(s) 115, 225, 297, 300
BlsI GCNGC 4 cut(s) 116, 226, 298, 301
BmsI GCATC 4 cut(s) 58, 80, 319, 541
BsaJI CCNNGG 3 cut(s) 131, 271, 573
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 1 cut(s) 421
Bse3DI GCAATG 2 cut(s) 219, 550
BseDI CCNNGG 3 cut(s) 131, 271, 573
BseGI GGATG 1 cut(s) 466
BseLI CCNNNNNNNGG 1 cut(s) 421
BseMI GCAATG 2 cut(s) 219, 550
BseMII CTCAG 1 cut(s) 107
BseRI GAGGAG 1 cut(s) 332
BseXI GCAGC 4 cut(s) 126, 211, 308, 311
BsgI GTGCAG 1 cut(s) 133
BshFI GGCC 1 cut(s) 578
BsiHKAI GWGCWC 1 cut(s) 345
BsiSI CCGG 1 cut(s) 165
BslI CCNNNNNNNGG 1 cut(s) 421
BsnI GGCC 1 cut(s) 578
Bsp1286I GDGCHC 1 cut(s) 345
Bsp143I GATC 3 cut(s) 83, 246, 383
Bsp19I CCATGG 1 cut(s) 271
BspANI GGCC 1 cut(s) 578
BspCNI CTCAG 1 cut(s) 106
BspHI TCATGA 1 cut(s) 249
BspMAI CTGCAG 1 cut(s) 298
BspMI ACCTGC 1 cut(s) 461
BspPI GGATC 2 cut(s) 78, 391
BsrDI GCAATG 2 cut(s) 219, 550
BssECI CCNNGG 3 cut(s) 131, 271, 573
BssMI GATC 3 cut(s) 83, 246, 383
BssT1I CCWWGG 1 cut(s) 271
BstAPI GCANNNNNTGC 1 cut(s) 203
BstDEI CTNAG 1 cut(s) 93
BstDSI CCRYGG 1 cut(s) 271
BstF5I GGATG 1 cut(s) 466
BstH2I RGCGCY 2 cut(s) 305, 557
BstHHI GCGC 3 cut(s) 304, 532, 556
BstKTI GATC 3 cut(s) 86, 249, 386
BstMBI GATC 3 cut(s) 83, 246, 383
BstMWI GCNNNNNNNGC 1 cut(s) 203
BstSFI CTRYAG 1 cut(s) 294
BstV1I GCAGC 4 cut(s) 126, 211, 308, 311
BstX2I RGATCY 1 cut(s) 83
BstYI RGATCY 1 cut(s) 83
BsuRI GGCC 1 cut(s) 578
BtgI CCRYGG 1 cut(s) 271
BtsCI GGATG 1 cut(s) 466
BtsI GCAGTG 1 cut(s) 201
BtsIMutI CAGTG 1 cut(s) 201
BveI ACCTGC 1 cut(s) 461
CciI TCATGA 1 cut(s) 249
CfoI GCGC 3 cut(s) 304, 532, 556
CseI GACGC 1 cut(s) 106
Csp6I GTAC 3 cut(s) 26, 97, 514
CviAII CATG 6 cut(s) 250, 272, 313, 371, 420, 457
CviJI RGCY 8 cut(s) 38, 136, 259, 276, 343, 397, 567, 578
CviKI_1 RGCY 8 cut(s) 38, 136, 259, 276, 343, 397, 567, 578
CviQI GTAC 3 cut(s) 26, 97, 514
DdeI CTNAG 1 cut(s) 93
DpnI GATC 3 cut(s) 85, 248, 385
DpnII GATC 3 cut(s) 83, 246, 383
Ecl136II GAGCTC 1 cut(s) 343
Eco130I CCWWGG 1 cut(s) 271
Eco24I GRGCYC 1 cut(s) 345
Eco32I GATATC 1 cut(s) 412
Eco47III AGCGCT 1 cut(s) 303
Eco53kI GAGCTC 1 cut(s) 343
EcoICRI GAGCTC 1 cut(s) 343
EcoRV GATATC 1 cut(s) 412
EcoT14I CCWWGG 1 cut(s) 271
EcoT22I ATGCAT 1 cut(s) 73
EcoT38I GRGCYC 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 271
FaeI CATG 6 cut(s) 253, 275, 316, 374, 423, 460
FalI AAGNNNNNCTT 1 cut(s) 29
FatI CATG 6 cut(s) 249, 271, 312, 370, 419, 456
FbaI TGATCA 1 cut(s) 246
Fnu4HI GCNGC 4 cut(s) 115, 225, 297, 300
FokI GGATG 1 cut(s) 473
FriOI GRGCYC 1 cut(s) 345
Fsp4HI GCNGC 4 cut(s) 115, 225, 297, 300
FspBI CTAG 2 cut(s) 78, 194
FspI TGCGCA 1 cut(s) 531
GlaI GCGC 3 cut(s) 303, 531, 555
GluI GCNGC 4 cut(s) 115, 225, 297, 300
HaeII RGCGCY 2 cut(s) 305, 557
HaeIII GGCC 1 cut(s) 578
HapII CCGG 1 cut(s) 165
HgaI GACGC 1 cut(s) 106
HhaI GCGC 3 cut(s) 304, 532, 556
Hin1II CATG 6 cut(s) 253, 275, 316, 374, 423, 460
Hin6I GCGC 3 cut(s) 302, 530, 554
HinP1I GCGC 3 cut(s) 302, 530, 554
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HinfI GANTC 3 cut(s) 290, 367, 499
HpaII CCGG 1 cut(s) 165
HphI GGTGA 1 cut(s) 338
Hpy166II GTNNAC 1 cut(s) 337
Hpy188I TCNGA 1 cut(s) 132
Hpy188III TCNNGA 3 cut(s) 65, 175, 250
Hpy8I GTNNAC 1 cut(s) 337
HpyAV CCTTC 2 cut(s) 249, 483
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 8 cut(s) 60, 71, 114, 296, 312, 434, 456, 543
HpyF10VI GCNNNNNNNGC 1 cut(s) 203
HpyF3I CTNAG 1 cut(s) 93
HpySE526I ACGT 1 cut(s) 213
Hsp92II CATG 6 cut(s) 253, 275, 316, 374, 423, 460
HspAI GCGC 3 cut(s) 302, 530, 554
Ksp22I TGATCA 1 cut(s) 246
Kzo9I GATC 3 cut(s) 83, 246, 383
LmnI GCTCC 1 cut(s) 348
LpnPI CCDG 4 cut(s) 78, 178, 379, 466
Lsp1109I GCAGC 4 cut(s) 126, 211, 308, 311
LweI GCATC 4 cut(s) 58, 80, 319, 541
MaeI CTAG 2 cut(s) 78, 194
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 2 cut(s) 118, 214
MalI GATC 3 cut(s) 85, 248, 385
MboI GATC 3 cut(s) 83, 246, 383
MflI RGATCY 1 cut(s) 83
MhlI GDGCHC 1 cut(s) 345
MluCI AATT 3 cut(s) 53, 362, 388
MlyI GAGTC 2 cut(s) 299, 376
MmeI TCCRAC 1 cut(s) 111
MnlI CCTC 5 cut(s) 126, 310, 439, 583, 589
Mph1103I ATGCAT 1 cut(s) 73
MseI TTAA 1 cut(s) 210
MslI CAYNNNNRTG 1 cut(s) 455
MspI CCGG 1 cut(s) 165
MwoI GCNNNNNNNGC 1 cut(s) 203
NcoI CCATGG 1 cut(s) 271
NdeII GATC 3 cut(s) 83, 246, 383
NlaIII CATG 6 cut(s) 253, 275, 316, 374, 423, 460
NmeAIII GCCGAG 1 cut(s) 554
NmuCI GTSAC 2 cut(s) 118, 214
NsbI TGCGCA 1 cut(s) 531
NsiI ATGCAT 1 cut(s) 73
PagI TCATGA 1 cut(s) 249
PaqCI CACCTGC 1 cut(s) 461
PcsI WCGNNNNNNNCGW 1 cut(s) 587
PfeI GAWTC 1 cut(s) 499
PkrI GCNGC 4 cut(s) 116, 226, 298, 301
PleI GAGTC 2 cut(s) 298, 375
PpsI GAGTC 2 cut(s) 298, 375
Psp124BI GAGCTC 1 cut(s) 345
PstI CTGCAG 1 cut(s) 298
PsuI RGATCY 1 cut(s) 83
RsaI GTAC 3 cut(s) 27, 98, 515
RsaNI GTAC 3 cut(s) 26, 97, 514
RseI CAYNNNNRTG 1 cut(s) 455
SacI GAGCTC 1 cut(s) 345
SaqAI TTAA 1 cut(s) 210
SatI GCNGC 4 cut(s) 115, 225, 297, 300
Sau3AI GATC 3 cut(s) 83, 246, 383
SchI GAGTC 2 cut(s) 299, 376
SduI GDGCHC 1 cut(s) 345
SetI ASST 5 cut(s) 216, 345, 455, 515, 569
SfaNI GCATC 4 cut(s) 58, 80, 319, 541
SfcI CTRYAG 1 cut(s) 294
SmiMI CAYNNNNRTG 1 cut(s) 455
Sse9I AATT 3 cut(s) 53, 362, 388
SspMI CTAG 2 cut(s) 78, 194
SstI GAGCTC 1 cut(s) 345
StyI CCWWGG 1 cut(s) 271
TaiI ACGT 1 cut(s) 216
TaqI TCGA 2 cut(s) 353, 581
TasI AATT 3 cut(s) 53, 362, 388
TatI WGTACW 2 cut(s) 25, 96
TfiI GAWTC 1 cut(s) 499
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TscAI CASTG 1 cut(s) 208
TseFI GTSAC 2 cut(s) 118, 214
TseI GCWGC 4 cut(s) 114, 224, 296, 299
Tsp45I GTSAC 2 cut(s) 118, 214
TspDTI ATGAA 2 cut(s) 375, 486
TspGWI ACGGA 1 cut(s) 497
TspRI CASTG 1 cut(s) 208
XapI RAATTY 1 cut(s) 388
XspI CTAG 2 cut(s) 78, 194
Zsp2I ATGCAT 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.