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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT4G23440 AT4G23440 |
| fragaria_vesca | FvH4_5g02740 FvH4_5g02740 FvH4_5g02740 FvH4_5g02740 |
| malus_domestica | MD06G1086700.v1.1 MD14G1107900.v1.1 |
| prunus_persica | Prupe.5G103200_v2.0.a1 Prupe.5G103200_v2.0.a1 |
| pyrus_communis | pycom06g08310 pycom14g10270 |
| rosa_chinensis | RchiOBHm_Chr7g0197191 |
| rosa_laevigata | RLG00000003954 RLG00000034513 |
| rosa_multiflora | Rmu_ssc0000465.1_g000018 |
| rosa_roxburghii | Rroxscaffold_3G00258610 |
| rosa_rugosa | Rorug07G0038200 Rorug07G0038300 Rorug07G0038400 |
| rosa_samantha | Rh7AG163100 Rh7BG165100 Rh7CG171100 Rh7DG164500 |
| rosa_wichuraiana | Rw7G014240 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1015 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 2543 |
| Acc36I | ACCTGC | 1 cut(s) | 1015 |
| AccB1I | GGYRCC | 2 cut(s) | 274, 2560 |
| AccII | CGCG | 2 cut(s) | 318, 2469 |
| AccIII | TCCGGA | 1 cut(s) | 1430 |
| AciI | CCGC | 8 cut(s) | 316, 985, 1222, 2184, 2467, 2469, 2659, 2743 |
| AclWI | GGATC | 7 cut(s) | 188, 439, 1728, 1746, 2374, 2479, 2574 |
| AcoI | YGGCCR | 1 cut(s) | 508 |
| AcsI | RAATTY | 6 cut(s) | 216, 1063, 1100, 1460, 1804, 2985 |
| AcuI | CTGAAG | 2 cut(s) | 60, 2925 |
| AfaI | GTAC | 3 cut(s) | 190, 1487, 2365 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 373, 784, 895, 1754 |
| AflIII | ACRYGT | 1 cut(s) | 264 |
| AgeI | ACCGGT | 1 cut(s) | 1267 |
| AjnI | CCWGG | 8 cut(s) | 23, 372, 445, 766, 2157, 2676, 2822, 2963 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1419, 1451 |
| AleI | CACNNNNGTG | 1 cut(s) | 1350 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2558, 2590 |
| Alw21I | GWGCWC | 3 cut(s) | 1155, 1659, 2764 |
| Alw26I | GTCTC | 3 cut(s) | 1757, 1997, 2251 |
| AlwI | GGATC | 7 cut(s) | 188, 439, 1728, 1746, 2374, 2479, 2574 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 30 |
| Ama87I | CYCGRG | 2 cut(s) | 894, 1641 |
| Aor13HI | TCCGGA | 1 cut(s) | 1430 |
| AoxI | GGCC | 3 cut(s) | 508, 954, 2744 |
| ApeKI | GCWGC | 7 cut(s) | 1631, 1975, 2213, 2504, 2642, 2684, 2936 |
| ApoI | RAATTY | 6 cut(s) | 216, 1063, 1100, 1460, 1804, 2985 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2577, 2609 |
| AseI | ATTAAT | 1 cut(s) | 1245 |
| AsiGI | ACCGGT | 1 cut(s) | 1267 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 683, 879, 1104 |
| AspA2I | CCTAGG | 1 cut(s) | 1166 |
| AspLEI | GCGC | 1 cut(s) | 320 |
| AspS9I | GGNCC | 7 cut(s) | 27, 376, 449, 742, 954, 2129, 2155 |
| AsuC2I | CCSGG | 3 cut(s) | 391, 2708, 2924 |
| AvaI | CYCGRG | 2 cut(s) | 894, 1641 |
| AvaII | GGWCC | 6 cut(s) | 27, 376, 449, 742, 2129, 2155 |
| AvrII | CCTAGG | 1 cut(s) | 1166 |
| BanI | GGYRCC | 2 cut(s) | 274, 2560 |
| BanII | GRGCYC | 2 cut(s) | 1155, 1659 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 1887, 1919, 2128, 2160 |
| BbsI | GAAGAC | 1 cut(s) | 60 |
| Bbv12I | GWGCWC | 3 cut(s) | 1155, 1659, 2764 |
| BbvI | GCAGC | 7 cut(s) | 1643, 1962, 2200, 2516, 2629, 2696, 2923 |
| BccI | CCATC | 3 cut(s) | 133, 920, 2120 |
| BceAI | ACGGC | 2 cut(s) | 495, 941 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 81, 115 |
| BciT130I | CCWGG | 8 cut(s) | 25, 374, 447, 768, 2159, 2678, 2824, 2965 |
| BciVI | GTATCC | 1 cut(s) | 595 |
| BcnI | CCSGG | 3 cut(s) | 391, 2708, 2924 |
| BcoDI | GTCTC | 3 cut(s) | 1757, 1997, 2251 |
| BfaI | CTAG | 8 cut(s) | 357, 726, 1161, 1167, 1368, 2210, 2648, 2816 |
| BfuAI | ACCTGC | 1 cut(s) | 1015 |
| BfuI | GTATCC | 1 cut(s) | 595 |
| BglII | AGATCT | 1 cut(s) | 593 |
| BisI | GCNGC | 9 cut(s) | 1632, 1976, 2214, 2467, 2505, 2643, 2685, 2744, 2937 |
| BlnI | CCTAGG | 1 cut(s) | 1166 |
| BlpI | GCTNAGC | 1 cut(s) | 2140 |
| BlsI | GCNGC | 9 cut(s) | 1633, 1977, 2215, 2468, 2506, 2644, 2686, 2745, 2938 |
| Bme18I | GGWCC | 6 cut(s) | 27, 376, 449, 742, 2129, 2155 |
| BmeT110I | CYCGRG | 2 cut(s) | 894, 1641 |
| BmgT120I | GGNCC | 7 cut(s) | 27, 376, 449, 742, 954, 2129, 2155 |
| BmiI | GGNNCC | 6 cut(s) | 276, 545, 744, 1812, 1840, 2562 |
| BmrI | ACTGGG | 1 cut(s) | 1934 |
| BmsI | GCATC | 5 cut(s) | 329, 357, 590, 1120, 1293 |
| BmuI | ACTGGG | 1 cut(s) | 1934 |
| BoxI | GACNNNNGTC | 1 cut(s) | 762 |
| BpiI | GAAGAC | 1 cut(s) | 60 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 37 |
| BpmI | CTGGAG | 3 cut(s) | 7, 190, 1857 |
| Bpu10I | CCTNAGC | 1 cut(s) | 200 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2140 |
| BpuEI | CTTGAG | 2 cut(s) | 781, 1139 |
| BpuMI | CCSGG | 3 cut(s) | 391, 2708, 2924 |
| BsaBI | GATNNNNATC | 2 cut(s) | 124, 2384 |
| BsaI | GGTCTC | 1 cut(s) | 1997 |
| BsaJI | CCNNGG | 8 cut(s) | 372, 1166, 1842, 2445, 2467, 2676, 2706, 2823 |
| BsaWI | WCCGGW | 2 cut(s) | 1267, 1430 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 928, 958 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 373, 784, 895, 1754 |
| Bse118I | RCCGGY | 2 cut(s) | 271, 1267 |
| Bse1I | ACTGG | 6 cut(s) | 278, 337, 1929, 2010, 2192, 2757 |
| Bse3DI | GCAATG | 6 cut(s) | 521, 1854, 2349, 2696, 2810, 2931 |
| Bse8I | GATNNNNATC | 2 cut(s) | 124, 2384 |
| BseAI | TCCGGA | 1 cut(s) | 1430 |
| BseBI | CCWGG | 8 cut(s) | 25, 374, 447, 768, 2159, 2678, 2824, 2965 |
| BseDI | CCNNGG | 8 cut(s) | 372, 1166, 1842, 2445, 2467, 2676, 2706, 2823 |
| BseGI | GGATG | 5 cut(s) | 348, 576, 649, 931, 1573 |
| BseJI | GATNNNNATC | 2 cut(s) | 124, 2384 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 373, 784, 895, 1754 |
| BseMI | GCAATG | 6 cut(s) | 521, 1854, 2349, 2696, 2810, 2931 |
| BseMII | CTCAG | 6 cut(s) | 150, 467, 704, 1962, 2054, 2777 |
| BseNI | ACTGG | 6 cut(s) | 278, 337, 1929, 2010, 2192, 2757 |
| BseRI | GAGGAG | 8 cut(s) | 493, 814, 819, 1229, 1640, 1931, 2436, 2645 |
| BseXI | GCAGC | 7 cut(s) | 1643, 1962, 2200, 2516, 2629, 2696, 2923 |
| Bsh1236I | CGCG | 2 cut(s) | 318, 2469 |
| BshFI | GGCC | 3 cut(s) | 510, 956, 2746 |
| BshNI | GGYRCC | 2 cut(s) | 274, 2560 |
| BshTI | ACCGGT | 1 cut(s) | 1267 |
| BsiHKAI | GWGCWC | 3 cut(s) | 1155, 1659, 2764 |
| BsiHKCI | CYCGRG | 2 cut(s) | 894, 1641 |
| BsiSI | CCGG | 7 cut(s) | 272, 390, 1268, 1431, 2564, 2708, 2924 |
| BslFI | GGGAC | 4 cut(s) | 728, 1941, 2420, 2862 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 373, 784, 895, 1754 |
| BsmAI | GTCTC | 3 cut(s) | 1757, 1997, 2251 |
| BsmBI | CGTCTC | 1 cut(s) | 2251 |
| BsmFI | GGGAC | 4 cut(s) | 728, 1941, 2420, 2862 |
| BsmI | GAATGC | 1 cut(s) | 2022 |
| BsnI | GGCC | 3 cut(s) | 510, 956, 2746 |
| Bso31I | GGTCTC | 1 cut(s) | 1997 |
| BsoBI | CYCGRG | 2 cut(s) | 894, 1641 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1155, 1659, 2764 |
| Bsp13I | TCCGGA | 1 cut(s) | 1430 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2140 |
| Bsp19I | CCATGG | 1 cut(s) | 2445 |
| BspACI | CCGC | 8 cut(s) | 316, 985, 1222, 2184, 2467, 2469, 2659, 2743 |
| BspANI | GGCC | 3 cut(s) | 510, 956, 2746 |
| BspCNI | CTCAG | 6 cut(s) | 151, 468, 705, 1963, 2055, 2776 |
| BspEI | TCCGGA | 1 cut(s) | 1430 |
| BspFNI | CGCG | 2 cut(s) | 318, 2469 |
| BspHI | TCATGA | 1 cut(s) | 1659 |
| BspLI | GGNNCC | 6 cut(s) | 276, 545, 744, 1812, 1840, 2562 |
| BspMI | ACCTGC | 1 cut(s) | 1015 |
| BspPI | GGATC | 7 cut(s) | 188, 439, 1728, 1746, 2374, 2479, 2574 |
| BspQI | GCTCTTC | 1 cut(s) | 1611 |
| BspT107I | GGYRCC | 2 cut(s) | 274, 2560 |
| BspTNI | GGTCTC | 1 cut(s) | 1997 |
| BsrDI | GCAATG | 6 cut(s) | 521, 1854, 2349, 2696, 2810, 2931 |
| BsrFI | RCCGGY | 2 cut(s) | 271, 1267 |
| BsrI | ACTGG | 6 cut(s) | 278, 337, 1929, 2010, 2192, 2757 |
| BssAI | RCCGGY | 2 cut(s) | 271, 1267 |
| BssECI | CCNNGG | 8 cut(s) | 372, 1166, 1842, 2445, 2467, 2676, 2706, 2823 |
| BssT1I | CCWWGG | 2 cut(s) | 1166, 2445 |
| Bst2UI | CCWGG | 8 cut(s) | 25, 374, 447, 768, 2159, 2678, 2824, 2965 |
| Bst4CI | ACNGT | 4 cut(s) | 1674, 2262, 2799, 2853 |
| Bst6I | CTCTTC | 1 cut(s) | 1611 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 281 |
| BstC8I | GCNNGC | 1 cut(s) | 2703 |
| BstDSI | CCRYGG | 2 cut(s) | 2445, 2467 |
| BstF5I | GGATG | 5 cut(s) | 348, 576, 649, 931, 1573 |
| BstFNI | CGCG | 2 cut(s) | 318, 2469 |
| BstHHI | GCGC | 1 cut(s) | 320 |
| BstMAI | GTCTC | 3 cut(s) | 1757, 1997, 2251 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 281, 412, 1093, 2183, 2213, 2681, 2693, 2702 |
| BstNI | CCWGG | 8 cut(s) | 25, 374, 447, 768, 2159, 2678, 2824, 2965 |
| BstNSI | RCATGY | 3 cut(s) | 268, 623, 2511 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 762 |
| BstUI | CGCG | 2 cut(s) | 318, 2469 |
| BstV1I | GCAGC | 7 cut(s) | 1643, 1962, 2200, 2516, 2629, 2696, 2923 |
| BstV2I | GAAGAC | 1 cut(s) | 60 |
| BstX2I | RGATCY | 5 cut(s) | 193, 593, 1738, 2379, 2566 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2126 |
| BstYI | RGATCY | 5 cut(s) | 193, 593, 1738, 2379, 2566 |
| BsuI | GTATCC | 1 cut(s) | 595 |
| BsuRI | GGCC | 3 cut(s) | 510, 956, 2746 |
| BtgI | CCRYGG | 2 cut(s) | 2445, 2467 |
| BtsCI | GGATG | 5 cut(s) | 348, 576, 649, 931, 1573 |
| BtsIMutI | CAGTG | 4 cut(s) | 465, 2060, 2764, 2795 |
| BveI | ACCTGC | 1 cut(s) | 1015 |
| Cac8I | GCNNGC | 1 cut(s) | 2703 |
| CaiI | CAGNNNCTG | 1 cut(s) | 30 |
| CciI | TCATGA | 1 cut(s) | 1659 |
| CfoI | GCGC | 1 cut(s) | 320 |
| Cfr10I | RCCGGY | 2 cut(s) | 271, 1267 |
| Cfr13I | GGNCC | 7 cut(s) | 27, 376, 449, 742, 954, 2129, 2155 |
| Cfr42I | CCGCGG | 1 cut(s) | 2470 |
| CseI | GACGC | 1 cut(s) | 1938 |
| CsiI | ACCWGGT | 1 cut(s) | 445 |
| Csp6I | GTAC | 3 cut(s) | 189, 1486, 2364 |
| CspAI | ACCGGT | 1 cut(s) | 1267 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1336, 1371 |
| CviQI | GTAC | 3 cut(s) | 189, 1486, 2364 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2543 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2543 |
| EaeI | YGGCCR | 1 cut(s) | 508 |
| Eam1104I | CTCTTC | 1 cut(s) | 1611 |
| EarI | CTCTTC | 1 cut(s) | 1611 |
| EciI | GGCGGA | 3 cut(s) | 1000, 1237, 2648 |
| Ecl136II | GAGCTC | 2 cut(s) | 1153, 1657 |
| Eco130I | CCWWGG | 2 cut(s) | 1166, 2445 |
| Eco24I | GRGCYC | 2 cut(s) | 1155, 1659 |
| Eco31I | GGTCTC | 1 cut(s) | 1997 |
| Eco32I | GATATC | 3 cut(s) | 7, 1475, 2554 |
| Eco47I | GGWCC | 6 cut(s) | 27, 376, 449, 742, 2129, 2155 |
| Eco53kI | GAGCTC | 2 cut(s) | 1153, 1657 |
| Eco57I | CTGAAG | 2 cut(s) | 60, 2925 |
| Eco88I | CYCGRG | 2 cut(s) | 894, 1641 |
| EcoICRI | GAGCTC | 2 cut(s) | 1153, 1657 |
| EcoO109I | RGGNCCY | 1 cut(s) | 27 |
| EcoRI | GAATTC | 2 cut(s) | 216, 1100 |
| EcoRII | CCWGG | 8 cut(s) | 23, 372, 445, 766, 2157, 2676, 2822, 2963 |
| EcoRV | GATATC | 3 cut(s) | 7, 1475, 2554 |
| EcoT14I | CCWWGG | 2 cut(s) | 1166, 2445 |
| EcoT22I | ATGCAT | 1 cut(s) | 256 |
| EcoT38I | GRGCYC | 2 cut(s) | 1155, 1659 |
| ErhI | CCWWGG | 2 cut(s) | 1166, 2445 |
| Esp3I | CGTCTC | 1 cut(s) | 2251 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 722, 754 |
| FaqI | GGGAC | 4 cut(s) | 728, 1941, 2420, 2862 |
| FauNDI | CATATG | 1 cut(s) | 1210 |
| Fnu4HI | GCNGC | 9 cut(s) | 1632, 1976, 2214, 2467, 2505, 2643, 2685, 2744, 2937 |
| FokI | GGATG | 5 cut(s) | 335, 583, 656, 938, 1580 |
| FriOI | GRGCYC | 2 cut(s) | 1155, 1659 |
| Fsp4HI | GCNGC | 9 cut(s) | 1632, 1976, 2214, 2467, 2505, 2643, 2685, 2744, 2937 |
| FspBI | CTAG | 8 cut(s) | 357, 726, 1161, 1167, 1368, 2210, 2648, 2816 |
| GlaI | GCGC | 1 cut(s) | 319 |
| GluI | GCNGC | 9 cut(s) | 1632, 1976, 2214, 2467, 2505, 2643, 2685, 2744, 2937 |
| GsuI | CTGGAG | 3 cut(s) | 7, 190, 1857 |
| HaeIII | GGCC | 3 cut(s) | 510, 956, 2746 |
| HapII | CCGG | 7 cut(s) | 272, 390, 1268, 1431, 2564, 2708, 2924 |
| HgaI | GACGC | 1 cut(s) | 1938 |
| HhaI | GCGC | 1 cut(s) | 320 |
| Hin6I | GCGC | 1 cut(s) | 318 |
| HinP1I | GCGC | 1 cut(s) | 318 |
| HincII | GTYRAC | 1 cut(s) | 1008 |
| HindII | GTYRAC | 1 cut(s) | 1008 |
| HindIII | AAGCTT | 2 cut(s) | 1609, 2137 |
| HinfI | GANTC | 8 cut(s) | 146, 230, 763, 1283, 1427, 1543, 2424, 2433 |
| HpaII | CCGG | 7 cut(s) | 272, 390, 1268, 1431, 2564, 2708, 2924 |
| Hpy166II | GTNNAC | 2 cut(s) | 515, 1008 |
| Hpy8I | GTNNAC | 2 cut(s) | 515, 1008 |
| HpyAV | CCTTC | 9 cut(s) | 528, 628, 1075, 1273, 1396, 1799, 1803, 1855, 2090 |
| HpyCH4III | ACNGT | 4 cut(s) | 1674, 2262, 2799, 2853 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 281, 412, 1093, 2183, 2213, 2681, 2693, 2702 |
| HspAI | GCGC | 1 cut(s) | 318 |
| Kpn2I | TCCGGA | 1 cut(s) | 1430 |
| KspI | CCGCGG | 1 cut(s) | 2470 |
| LguI | GCTCTTC | 1 cut(s) | 1611 |
| LmnI | GCTCC | 8 cut(s) | 26, 209, 480, 549, 1084, 1316, 1810, 1838 |
| Lsp1109I | GCAGC | 7 cut(s) | 1643, 1962, 2200, 2516, 2629, 2696, 2923 |
| LweI | GCATC | 5 cut(s) | 329, 357, 590, 1120, 1293 |
| MabI | ACCWGGT | 1 cut(s) | 445 |
| MaeI | CTAG | 8 cut(s) | 357, 726, 1161, 1167, 1368, 2210, 2648, 2816 |
| MaeIII | GTNAC | 7 cut(s) | 170, 267, 359, 460, 958, 1120, 2864 |
| MfeI | CAATTG | 2 cut(s) | 624, 1950 |
| MflI | RGATCY | 5 cut(s) | 193, 593, 1738, 2379, 2566 |
| MhlI | GDGCHC | 3 cut(s) | 1155, 1659, 2764 |
| MlyI | GAGTC | 4 cut(s) | 772, 1292, 2433, 2442 |
| MmeI | TCCRAC | 1 cut(s) | 612 |
| Mph1103I | ATGCAT | 1 cut(s) | 256 |
| MroI | TCCGGA | 1 cut(s) | 1430 |
| MroXI | GAANNNNTTC | 3 cut(s) | 683, 879, 1104 |
| MseI | TTAA | 9 cut(s) | 53, 537, 950, 1245, 1605, 2024, 2339, 2541, 2768 |
| MslI | CAYNNNNRTG | 5 cut(s) | 366, 1350, 2124, 2251, 2388 |
| MspA1I | CMGCKG | 1 cut(s) | 2469 |
| MspI | CCGG | 7 cut(s) | 272, 390, 1268, 1431, 2564, 2708, 2924 |
| MunI | CAATTG | 2 cut(s) | 624, 1950 |
| Mva1269I | GAATGC | 1 cut(s) | 2022 |
| MvaI | CCWGG | 8 cut(s) | 25, 374, 447, 768, 2159, 2678, 2824, 2965 |
| MvnI | CGCG | 2 cut(s) | 318, 2469 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 281, 412, 1093, 2183, 2213, 2681, 2693, 2702 |
| NciI | CCSGG | 3 cut(s) | 391, 2708, 2924 |
| NcoI | CCATGG | 1 cut(s) | 2445 |
| NdeI | CATATG | 1 cut(s) | 1210 |
| NlaIV | GGNNCC | 6 cut(s) | 276, 545, 744, 1812, 1840, 2562 |
| NmeAIII | GCCGAG | 1 cut(s) | 1867 |
| NmuCI | GTSAC | 7 cut(s) | 170, 267, 359, 460, 958, 1120, 2864 |
| NsiI | ATGCAT | 1 cut(s) | 256 |
| NspI | RCATGY | 3 cut(s) | 268, 623, 2511 |
| OliI | CACNNNNGTG | 1 cut(s) | 1350 |
| PaeR7I | CTCGAG | 1 cut(s) | 1641 |
| PagI | TCATGA | 1 cut(s) | 1659 |
| PaqCI | CACCTGC | 1 cut(s) | 1015 |
| PciI | ACATGT | 1 cut(s) | 264 |
| PciSI | GCTCTTC | 1 cut(s) | 1611 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2266 |
| PctI | GAATGC | 1 cut(s) | 2022 |
| PdmI | GAANNNNTTC | 3 cut(s) | 683, 879, 1104 |
| PfeI | GAWTC | 4 cut(s) | 146, 230, 1427, 1543 |
| PinAI | ACCGGT | 1 cut(s) | 1267 |
| PkrI | GCNGC | 9 cut(s) | 1633, 1977, 2215, 2468, 2506, 2644, 2686, 2745, 2938 |
| PleI | GAGTC | 4 cut(s) | 771, 1291, 2432, 2441 |
| PpsI | GAGTC | 4 cut(s) | 771, 1291, 2432, 2441 |
| PpuMI | RGGWCCY | 1 cut(s) | 27 |
| PscI | ACATGT | 1 cut(s) | 264 |
| PshAI | GACNNNNGTC | 1 cut(s) | 762 |
| PshBI | ATTAAT | 1 cut(s) | 1245 |
| Psp124BI | GAGCTC | 2 cut(s) | 1155, 1659 |
| Psp5II | RGGWCCY | 1 cut(s) | 27 |
| Psp6I | CCWGG | 8 cut(s) | 23, 372, 445, 766, 2157, 2676, 2822, 2963 |
| PspGI | CCWGG | 8 cut(s) | 23, 372, 445, 766, 2157, 2676, 2822, 2963 |
| PspN4I | GGNNCC | 6 cut(s) | 276, 545, 744, 1812, 1840, 2562 |
| PspPI | GGNCC | 7 cut(s) | 27, 376, 449, 742, 954, 2129, 2155 |
| PspPPI | RGGWCCY | 1 cut(s) | 27 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1189, 1221 |
| PstNI | CAGNNNCTG | 1 cut(s) | 30 |
| PsuI | RGATCY | 5 cut(s) | 193, 593, 1738, 2379, 2566 |
| RsaI | GTAC | 3 cut(s) | 190, 1487, 2365 |
| RsaNI | GTAC | 3 cut(s) | 189, 1486, 2364 |
| RseI | CAYNNNNRTG | 5 cut(s) | 366, 1350, 2124, 2251, 2388 |
| SacI | GAGCTC | 2 cut(s) | 1155, 1659 |
| SacII | CCGCGG | 1 cut(s) | 2470 |
| SapI | GCTCTTC | 1 cut(s) | 1611 |
| SaqAI | TTAA | 9 cut(s) | 53, 537, 950, 1245, 1605, 2024, 2339, 2541, 2768 |
| SatI | GCNGC | 9 cut(s) | 1632, 1976, 2214, 2467, 2505, 2643, 2685, 2744, 2937 |
| Sau96I | GGNCC | 7 cut(s) | 27, 376, 449, 742, 954, 2129, 2155 |
| SchI | GAGTC | 4 cut(s) | 772, 1292, 2433, 2442 |
| SduI | GDGCHC | 3 cut(s) | 1155, 1659, 2764 |
| SexAI | ACCWGGT | 1 cut(s) | 445 |
| SfaNI | GCATC | 5 cut(s) | 329, 357, 590, 1120, 1293 |
| Sfr274I | CTCGAG | 1 cut(s) | 1641 |
| Sfr303I | CCGCGG | 1 cut(s) | 2470 |
| SgrBI | CCGCGG | 1 cut(s) | 2470 |
| SinI | GGWCC | 6 cut(s) | 27, 376, 449, 742, 2129, 2155 |
| SlaI | CTCGAG | 1 cut(s) | 1641 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 366, 1350, 2124, 2251, 2388 |
| SmlI | CTYRAG | 3 cut(s) | 760, 1154, 1641 |
| SmoI | CTYRAG | 3 cut(s) | 760, 1154, 1641 |
| SsiI | CCGC | 8 cut(s) | 316, 985, 1222, 2184, 2467, 2469, 2659, 2743 |
| SspI | AATATT | 1 cut(s) | 1965 |
| SspMI | CTAG | 8 cut(s) | 357, 726, 1161, 1167, 1368, 2210, 2648, 2816 |
| SstI | GAGCTC | 2 cut(s) | 1155, 1659 |
| StyI | CCWWGG | 2 cut(s) | 1166, 2445 |
| TaaI | ACNGT | 4 cut(s) | 1674, 2262, 2799, 2853 |
| TaqI | TCGA | 6 cut(s) | 166, 598, 1054, 1642, 2856, 2903 |
| TatI | WGTACW | 1 cut(s) | 188 |
| TauI | GCSGC | 2 cut(s) | 2469, 2746 |
| TfiI | GAWTC | 4 cut(s) | 146, 230, 1427, 1543 |
| Tru1I | TTAA | 9 cut(s) | 53, 537, 950, 1245, 1605, 2024, 2339, 2541, 2768 |
| Tru9I | TTAA | 9 cut(s) | 53, 537, 950, 1245, 1605, 2024, 2339, 2541, 2768 |
| TscAI | CASTG | 4 cut(s) | 465, 2067, 2764, 2802 |
| TseFI | GTSAC | 7 cut(s) | 170, 267, 359, 460, 958, 1120, 2864 |
| TseI | GCWGC | 7 cut(s) | 1631, 1975, 2213, 2504, 2642, 2684, 2936 |
| Tsp45I | GTSAC | 7 cut(s) | 170, 267, 359, 460, 958, 1120, 2864 |
| TspGWI | ACGGA | 3 cut(s) | 441, 1604, 1921 |
| TspRI | CASTG | 4 cut(s) | 465, 2067, 2764, 2802 |
| VpaK11BI | GGWCC | 6 cut(s) | 27, 376, 449, 742, 2129, 2155 |
| VspI | ATTAAT | 1 cut(s) | 1245 |
| XapI | RAATTY | 6 cut(s) | 216, 1063, 1100, 1460, 1804, 2985 |
| XbaI | TCTAGA | 1 cut(s) | 2647 |
| XceI | RCATGY | 3 cut(s) | 268, 623, 2511 |
| XhoI | CTCGAG | 1 cut(s) | 1641 |
| XmaJI | CCTAGG | 1 cut(s) | 1166 |
| XmnI | GAANNNNTTC | 3 cut(s) | 683, 879, 1104 |
| XspI | CTAG | 8 cut(s) | 357, 726, 1161, 1167, 1368, 2210, 2648, 2816 |
| Zsp2I | ATGCAT | 1 cut(s) | 256 |