MD06G1086700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007429
Physical Location & Seq
Forward (+)
21350633 .. 21354858
4226 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1086700.v1.1.491

Sequence Viewer

Length: 2997 bp
ATGGATATGGGAGATGATAGCTCCAGGTTCTGTTCCTTACCGGCTACAACTTCGAGGAATATTTCAACCTCATCTTCGGCATTCTTTTCAGCAAATCAGTCGCCTTTCTTTTCCCCAAGATCGCCTTCTTGTCAACTATCTGAATCAACGAGGTCCGATGCTCCATGTGACAGCATGGTTTTAAGTACAGACCCCCTTAGTTCCAGCTCTGGAATTCCAGACCTTGAATCTCTATCAAACATCAGATATAAATTGGCAAACTTATCACCGGCCCCAGCTGCATCAGTTTCAGGCGATTTTGAGAAGTTTGATCGCGTGTCTTCCTCAACAGCCATTTCTAACAGTATTCTATCTTGTCAGAGCCATGCCCGGGCATATGATTATTCTGGGCAAAAAGAGAGGCAGAGAAAGCATGGGAGAAACTATGGAGCCTCATATACAACAGGTCCAGCTTCAATGACATCTAACAGACTGAGGAGCTGTGATATTTTCATAGGTTTGCATGGCCGTAAACCTTCTTTGCTGAGGTTTGCTAATTGGCTCCGAGTTGAGTTGGAAGTTCAGGGGATGAGTTGCTTTGTATCTGATAGAGCTCGATGTAGGAACTCCTGCAAACATGGAATTGTTGAGAGGGCCATGGATGTTTCTTCTTTTGGGATTGTCATCTTAACAAGGAAGTCATTCCGAAATCCATACACCATCGAGGAACTGCGATTTTTCGCTAGCAAGAAGAACTTGGTCCCAATATTCTATGATTTGAGTCCAGGTGATTGCCTTGTCAGGGATATCGTTGAGAAGAGAGGAGAGCTGTGGGAAAAACATGGGGGAGAATTATGGATTTTGTATGGAGGGCTTGAGAAGGAGTGGAAAGAAGCTCTTCATAGCCTCTCTCGGGTGGATGAGTGGAAATTGGAGGCTCAGGATGGTAACTGGAGAGATTGTATATTAAGGGCTGTCACATTATTAGCAATTAGATTAGGGAGGAGAAGTGTTGTAGATCGGTTAAAGAAGTGGAGAGAGGTGGTGGATAAAGAGGAGTTCCCTTTCCCTCGAAATGAGAACTTCGTTGGCAGGAAGAAGGAACTGTCTGAGCTGGAATTCATACTTTTTGGCAATGTCAGTGGAGACGCAGAAAGAGATTATTTTGAGCTTAAGGCTAGGCCTAGACGAAAGAACTTGACAATTGGGTGGGGTAGGAGCAGTTCATTTGACGAAAGGCGAAGGGAACGAAAACTGGAGAGTGGAAGCAGAAAGGGAAAAGAACCAGTTGTGTGGAAGGAATCAGAGAAAGAGATTGAGTTGCAAAGCACAGAAAATCCTCAAAGACAATCAAAGCCAAAAAGTGGTGGAAGATATTCAAGGAGGAAAAGATCAATGAAGGTTGTGTATGGAAAGGGGATTGCTTGTGTGTCTGGGGACTCGGGAATTGGCAAGACGGAACTTCTTCTCGAATTTGCTTACAGACATCACCAGAGGTACAAGATGGTTTTATGGATAGGAGGGGAGAGTAGGTATATTAGGCAAAACTATTTGAATCTCTGGTCATTTCTAGAAGTTGATGTAGGGGTTGAAAATTGCTTTGACAAAAACAGAATCAAAAGCTTTGAAGAACAGGAAGATGCAGCCATAGCTAGAGTACGCAGAGAACTCATGAGAAACATGCCATTTTTGGTAGTGATTGATAACTTAGAAAGTGAAAAGGATTGGTGGGATCATAAACTTGTAATGGATCTTCTTCCCCGTTTTGGCGGTGATACACACATAATAATATCCACATGCCTTCCTTCTGTGATGAATTTGGAGCCCTTGAAACTCTCATTCTTATCCGGGGCTGAGGCAATGTCGCTTATGCGGGGTAGTGTAAAAGAATACACAGAAAATGAAGAATTGGATGCTTTACGGGCTATTGAGGAGAAAGTGGGACGCTCCACCTTGGGACTTTCTATTGTAGGTGCAATATTGTCCGAGCTGCCCATACTTCCAAGTAAGCTGTTGGAGACAACTAGTAGAATGCCCTTAAAAGAATTTTCATGGAGTTGCAGGGAGACTAATTCGTTGAGGCGGCATACTTTCCTTCTGCAACTCTTCGAGGTGTGTTTTTCAATTTTTGATCATGCAGATGGTCCAAGGAGCTTGGCAACGAGAATGGTCCAGGCAAGTACTTGGTTTGCACCGGCCGCAATTCCAGTTTCTTTGTTGGCCCAAGCTGCTCACAAAATACCAGAAAAGCATCAAGGGACGTGGTTATGGAGAAAGCTGATGAATTCTCTAACTTGTGGCTTTACTTCATCATACACCAAAAAATCAGAAGCAGATGCGACTTCCCTGTTGTTAAGGTTTAACATTGCTAGAAGCAGCACCAAACAAGACCATATCCATTTCCATGAACTCGTCAAGCTTTATGCTCGCATGAGAGTAGTGACCAGAGTTGCACAAGCGATGGTTCAAGCTGTCATCACTCATGGATCGATATCTCAACACTCGGAACATATCTGGGCAGCCTGTTTCTTGATATTTGGATTCAGCCATGACCCTGCAGTCGTTGAACTTAAGGTGTCGGACCTGCTGTACCTTGTCAAGGAAGTGGTGTTGCCTCTTGCCATCCGGACCTTCATAACATTCTCTCGTTGCAATGCTGCCCTAGAACTCCTCCGCCTATGCACCAACGCCCTGGAAGCAGCAGACCAAGCGTTCGTAACCCCAGTCGAGAAGTGGCTCGATAAATCTCTTTGTTGGAGGCCGATCCAGACTAATGCTCAGCTGAATCCTTACCTTTGGCAGGAGCTCGCGCTATCAAGAGCCACAGTGCTGGAAACTAGGGCAAAGCTGATGCTAAGAGGGGGACAATTCGACATAGCAGATGATCTAATTAGGAAGGCGCTTTTTATCAGAACTTCAATTTGTGGTGAAGATCACCAAGACACCGTAGCTGCTCGTGAAACTCTTGGCAAAATCACCAGGCTTCTTGCAAATGTTCGTATTCATACTTCACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR TIR_2

Protein Analysis

999

Amino Acids

113.47

Weight (kDa)

9.12

Isoelectric Point (pI)

50.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_plant_disease PF25895 702 - 813 9.3e-46 Winged Helix Domain in plant disease resistance proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2537
Acc36I ACCTGC 1 cut(s) 2571
AccB7I CCANNNNNTGG 1 cut(s) 1343
AccII CGCG 2 cut(s) 315, 2789
AccIII TCCGGA 1 cut(s) 2604
AciI CCGC 5 cut(s) 1749, 1852, 2062, 2178, 2653
AclWI GGATC 4 cut(s) 1719, 1737, 2473, 2737
AcoI YGGCCR 2 cut(s) 505, 2175
AcsI RAATTY 6 cut(s) 213, 1097, 1451, 1795, 2024, 2263
AfaI GTAC 5 cut(s) 187, 1478, 1638, 2161, 2570
AfiI CCNNNNNNNGG 7 cut(s) 370, 781, 892, 1343, 1745, 2578, 2779
AflII CTTAAG 2 cut(s) 1151, 2549
AhlI ACTAGT 1 cut(s) 2003
AjiI CACGTC 1 cut(s) 2241
AjnI CCWGG 5 cut(s) 23, 763, 2151, 2670, 2957
AjuI GAANNNNNNNTTGG 2 cut(s) 109, 141
Alw21I GWGCWC 2 cut(s) 595, 2787
Alw26I GTCTC 3 cut(s) 1119, 1991, 2039
AlwI GGATC 4 cut(s) 1719, 1737, 2473, 2737
AlwNI CAGNNNCTG 1 cut(s) 30
Ama87I CYCGRG 3 cut(s) 369, 891, 1420
Aor13HI TCCGGA 1 cut(s) 2604
AoxI GGCC 7 cut(s) 270, 505, 633, 1160, 2175, 2199, 2738
ApeKI GCWGC 9 cut(s) 278, 1622, 1969, 2207, 2355, 2498, 2636, 2678, 2930
ApoI RAATTY 6 cut(s) 213, 1097, 1451, 1795, 2024, 2263
ArsI GACNNNNNNTTYG 2 cut(s) 2675, 2707
Asp700I GAANNNNTTC 4 cut(s) 680, 876, 1354, 1443
AspLEI GCGC 2 cut(s) 2791, 2881
AsuC2I CCSGG 3 cut(s) 370, 371, 1828
AsuHPI GGTGA 8 cut(s) 258, 779, 1460, 1763, 2906, 2918, 2947, 2982
AsuNHI GCTAGC 1 cut(s) 722
AvaI CYCGRG 3 cut(s) 369, 891, 1420
AvaII GGWCC 7 cut(s) 153, 446, 739, 2123, 2149, 2560, 2607
BanII GRGCYC 3 cut(s) 595, 1806, 2787
BarI GAAGNNNNNNTAC 3 cut(s) 1716, 1748, 2971
BauI CACGAG 1 cut(s) 2934
BbsI GAAGAC 1 cut(s) 312
Bbv12I GWGCWC 2 cut(s) 595, 2787
BbvCI CCTCAGC 2 cut(s) 524, 1833
BbvI GCAGC 9 cut(s) 265, 1634, 1956, 2194, 2367, 2510, 2623, 2690, 2917
BccI CCATC 6 cut(s) 707, 917, 1477, 2114, 2434, 2609
BceAI ACGGC 1 cut(s) 492
BcgI CGANNNNNNTGC 2 cut(s) 81, 115
BciT130I CCWGG 5 cut(s) 25, 765, 2153, 2672, 2959
BclI TGATCA 1 cut(s) 2110
BcnI CCSGG 3 cut(s) 370, 371, 1828
BcoDI GTCTC 3 cut(s) 1119, 1991, 2039
BcuI ACTAGT 1 cut(s) 2003
BfaI CTAG 9 cut(s) 723, 1158, 1164, 1550, 1632, 2004, 2349, 2642, 2817
BfmI CTRYAG 1 cut(s) 2535
BfoI RGCGCY 1 cut(s) 2882
BfrI CTTAAG 2 cut(s) 1151, 2549
BfuAI ACCTGC 1 cut(s) 2571
BlpI GCTNAGC 1 cut(s) 2757
BmcAI AGTACT 1 cut(s) 2161
Bme1390I CCNGG 8 cut(s) 25, 370, 371, 765, 1828, 2153, 2672, 2959
Bme18I GGWCC 7 cut(s) 153, 446, 739, 2123, 2149, 2560, 2607
BmeT110I CYCGRG 3 cut(s) 369, 891, 1420
BmgBI CACGTC 1 cut(s) 2241
BmiI GGNNCC 5 cut(s) 273, 430, 542, 741, 1803
BmrFI CCNGG 8 cut(s) 25, 370, 371, 765, 1828, 2153, 2672, 2959
BmrI ACTGGG 1 cut(s) 2696
BmsI GCATC 7 cut(s) 148, 290, 1609, 1882, 2239, 2305, 2820
BmtI GCTAGC 1 cut(s) 726
BmuI ACTGGG 1 cut(s) 2696
BpiI GAAGAC 1 cut(s) 312
BpmI CTGGAG 3 cut(s) 7, 952, 1256
Bpu10I CCTNAGC 3 cut(s) 524, 918, 1833
Bpu1102I GCTNAGC 1 cut(s) 2757
BpuEI CTTGAG 1 cut(s) 875
BpuMI CCSGG 3 cut(s) 370, 371, 1828
Bsa29I ATCGAT 1 cut(s) 2468
BsaBI GATNNNNATC 2 cut(s) 662, 2470
BsaJI CCNNGG 6 cut(s) 369, 636, 1827, 1932, 2126, 2670
BsaWI WCCGGW 1 cut(s) 2604
BsaXI ACNNNNNCTCC 6 cut(s) 925, 955, 973, 1003, 1496, 1526
Bsc4I CCNNNNNNNGG 7 cut(s) 370, 781, 892, 1343, 1745, 2578, 2779
Bse118I RCCGGY 3 cut(s) 40, 268, 2173
Bse1I ACTGG 5 cut(s) 935, 1239, 1265, 2186, 2702
Bse3DI GCAATG 4 cut(s) 1120, 1845, 2343, 2638
Bse8I GATNNNNATC 2 cut(s) 662, 2470
BseAI TCCGGA 1 cut(s) 2604
BseBI CCWGG 5 cut(s) 25, 765, 2153, 2672, 2959
BseCI ATCGAT 1 cut(s) 2468
BseDI CCNNGG 6 cut(s) 369, 636, 1827, 1932, 2126, 2670
BseGI GGATG 6 cut(s) 573, 646, 904, 928, 1897, 2601
BseJI GATNNNNATC 2 cut(s) 662, 2470
BseLI CCNNNNNNNGG 7 cut(s) 370, 781, 892, 1343, 1745, 2578, 2779
BseMI GCAATG 4 cut(s) 1120, 1845, 2343, 2638
BseMII CTCAG 6 cut(s) 464, 515, 932, 1080, 1824, 2771
BseNI ACTGG 5 cut(s) 935, 1239, 1265, 2186, 2702
BseRI GAGGAG 6 cut(s) 490, 816, 997, 1049, 1925, 2639
BseX3I CGGCCG 1 cut(s) 2175
BseXI GCAGC 9 cut(s) 265, 1634, 1956, 2194, 2367, 2510, 2623, 2690, 2917
BseYI CCCAGC 1 cut(s) 274
Bsh1236I CGCG 2 cut(s) 315, 2789
Bsh1285I CGRYCG 1 cut(s) 2178
BshFI GGCC 7 cut(s) 272, 507, 635, 1162, 2177, 2201, 2740
BshVI ATCGAT 1 cut(s) 2468
BsiEI CGRYCG 1 cut(s) 2178
BsiHKAI GWGCWC 2 cut(s) 595, 2787
BsiHKCI CYCGRG 3 cut(s) 369, 891, 1420
BsiSI CCGG 6 cut(s) 41, 269, 370, 1827, 2174, 2605
BslFI GGGAC 6 cut(s) 725, 1430, 1935, 1950, 2251, 2856
BslI CCNNNNNNNGG 7 cut(s) 370, 781, 892, 1343, 1745, 2578, 2779
BsmAI GTCTC 3 cut(s) 1119, 1991, 2039
BsmBI CGTCTC 1 cut(s) 1119
BsmFI GGGAC 6 cut(s) 725, 1430, 1935, 1950, 2251, 2856
BsmI GAATGC 2 cut(s) 80, 2016
BsnI GGCC 7 cut(s) 272, 507, 635, 1162, 2177, 2201, 2740
BsoBI CYCGRG 3 cut(s) 369, 891, 1420
Bsp1286I GDGCHC 3 cut(s) 595, 1806, 2787
Bsp13I TCCGGA 1 cut(s) 2604
Bsp1720I GCTNAGC 1 cut(s) 2757
Bsp19I CCATGG 1 cut(s) 636
BspACI CCGC 5 cut(s) 1749, 1852, 2062, 2178, 2653
BspANI GGCC 7 cut(s) 272, 507, 635, 1162, 2177, 2201, 2740
BspCNI CTCAG 6 cut(s) 465, 516, 931, 1081, 1825, 2770
BspDI ATCGAT 1 cut(s) 2468
BspEI TCCGGA 1 cut(s) 2604
BspFNI CGCG 2 cut(s) 315, 2789
BspHI TCATGA 1 cut(s) 1650
BspLI GGNNCC 5 cut(s) 273, 430, 542, 741, 1803
BspMAI CTGCAG 1 cut(s) 2539
BspMI ACCTGC 1 cut(s) 2571
BspOI GCTAGC 1 cut(s) 726
BspPI GGATC 4 cut(s) 1719, 1737, 2473, 2737
BspQI GCTCTTC 1 cut(s) 882
BspTI CTTAAG 2 cut(s) 1151, 2549
BsrDI GCAATG 4 cut(s) 1120, 1845, 2343, 2638
BsrFI RCCGGY 3 cut(s) 40, 268, 2173
BsrI ACTGG 5 cut(s) 935, 1239, 1265, 2186, 2702
BssAI RCCGGY 3 cut(s) 40, 268, 2173
BssECI CCNNGG 6 cut(s) 369, 636, 1827, 1932, 2126, 2670
BssSI CACGAG 1 cut(s) 2934
BssT1I CCWWGG 3 cut(s) 636, 1932, 2126
Bst2BI CACGAG 1 cut(s) 2934
Bst2UI CCWGG 5 cut(s) 25, 765, 2153, 2672, 2959
Bst4CI ACNGT 4 cut(s) 344, 1086, 2806, 2926
Bst6I CTCTTC 3 cut(s) 791, 882, 2090
BstAFI CTTAAG 2 cut(s) 1151, 2549
BstC8I GCNNGC 3 cut(s) 724, 2407, 2787
BstDEI CTNAG 9 cut(s) 197, 473, 524, 918, 1089, 1687, 1833, 2757, 2834
BstDSI CCRYGG 1 cut(s) 636
BstENI CCTNNNNNAGG 2 cut(s) 2576, 2777
BstF5I GGATG 6 cut(s) 573, 646, 904, 928, 1897, 2601
BstFNI CGCG 2 cut(s) 315, 2789
BstH2I RGCGCY 1 cut(s) 2882
BstHHI GCGC 2 cut(s) 2791, 2881
BstMAI GTCTC 3 cut(s) 1119, 1991, 2039
BstMCI CGRYCG 1 cut(s) 2178
BstMWI GCNNNNNNNGC 8 cut(s) 278, 409, 1628, 1901, 2177, 2207, 2675, 2687
BstNI CCWGG 5 cut(s) 25, 765, 2153, 2672, 2959
BstNSI RCATGY 2 cut(s) 1663, 1779
BstSCI CCNGG 8 cut(s) 23, 368, 369, 763, 1826, 2151, 2670, 2957
BstSFI CTRYAG 1 cut(s) 2535
BstUI CGCG 2 cut(s) 315, 2789
BstV1I GCAGC 9 cut(s) 265, 1634, 1956, 2194, 2367, 2510, 2623, 2690, 2917
BstV2I GAAGAC 1 cut(s) 312
BstX2I RGATCY 1 cut(s) 1729
BstXI CCANNNNNNTGG 3 cut(s) 1272, 2671, 2809
BstYI RGATCY 1 cut(s) 1729
BstZI CGGCCG 1 cut(s) 2175
Bsu15I ATCGAT 1 cut(s) 2468
BsuRI GGCC 7 cut(s) 272, 507, 635, 1162, 2177, 2201, 2740
BsuTUI ATCGAT 1 cut(s) 2468
BtgI CCRYGG 1 cut(s) 636
BtgZI GCGATG 1 cut(s) 2453
BtrI CACGTC 1 cut(s) 2241
BtsCI GGATG 6 cut(s) 573, 646, 904, 928, 1897, 2601
BtsIMutI CAGTG 2 cut(s) 1126, 2811
BveI ACCTGC 1 cut(s) 2571
Cac8I GCNNGC 3 cut(s) 724, 2407, 2787
CaiI CAGNNNCTG 1 cut(s) 30
CciI TCATGA 1 cut(s) 1650
CfoI GCGC 2 cut(s) 2791, 2881
Cfr10I RCCGGY 3 cut(s) 40, 268, 2173
Cfr9I CCCGGG 1 cut(s) 369
ClaI ATCGAT 1 cut(s) 2468
CseI GACGC 2 cut(s) 1136, 1932
Csp6I GTAC 5 cut(s) 186, 1477, 1637, 2160, 2569
CspCI CAANNNNNGTGG 4 cut(s) 1102, 1137, 2222, 2257
CviQI GTAC 5 cut(s) 186, 1477, 1637, 2160, 2569
DdeI CTNAG 9 cut(s) 197, 473, 524, 918, 1089, 1687, 1833, 2757, 2834
DrdI GACNNNNNNGTC 1 cut(s) 2537
DseDI GACNNNNNNGTC 1 cut(s) 2537
EaeI YGGCCR 2 cut(s) 505, 2175
EagI CGGCCG 1 cut(s) 2175
Eam1104I CTCTTC 3 cut(s) 791, 882, 2090
EarI CTCTTC 3 cut(s) 791, 882, 2090
EciI GGCGGA 1 cut(s) 2642
Ecl136II GAGCTC 2 cut(s) 593, 2785
EclXI CGGCCG 1 cut(s) 2175
Eco130I CCWWGG 3 cut(s) 636, 1932, 2126
Eco147I AGGCCT 1 cut(s) 1162
Eco24I GRGCYC 3 cut(s) 595, 1806, 2787
Eco32I GATATC 2 cut(s) 787, 2472
Eco47I GGWCC 7 cut(s) 153, 446, 739, 2123, 2149, 2560, 2607
Eco52I CGGCCG 1 cut(s) 2175
Eco53kI GAGCTC 2 cut(s) 593, 2785
Eco88I CYCGRG 3 cut(s) 369, 891, 1420
EcoICRI GAGCTC 2 cut(s) 593, 2785
EcoNI CCTNNNNNAGG 2 cut(s) 2576, 2777
EcoRI GAATTC 3 cut(s) 213, 1097, 2263
EcoRII CCWGG 5 cut(s) 23, 763, 2151, 2670, 2957
EcoRV GATATC 2 cut(s) 787, 2472
EcoT14I CCWWGG 3 cut(s) 636, 1932, 2126
EcoT38I GRGCYC 3 cut(s) 595, 1806, 2787
ErhI CCWWGG 3 cut(s) 636, 1932, 2126
Esp3I CGTCTC 1 cut(s) 1119
FalI AAGNNNNNCTT 6 cut(s) 109, 141, 719, 751, 861, 893
FaqI GGGAC 6 cut(s) 725, 1430, 1935, 1950, 2251, 2856
FauI CCCGC 1 cut(s) 1845
FauNDI CATATG 1 cut(s) 376
FbaI TGATCA 1 cut(s) 2110
FokI GGATG 6 cut(s) 580, 653, 911, 935, 1904, 2588
FriOI GRGCYC 3 cut(s) 595, 1806, 2787
FspBI CTAG 9 cut(s) 723, 1158, 1164, 1550, 1632, 2004, 2349, 2642, 2817
GlaI GCGC 2 cut(s) 2790, 2880
GsaI CCCAGC 1 cut(s) 278
GsuI CTGGAG 3 cut(s) 7, 952, 1256
HaeII RGCGCY 1 cut(s) 2882
HaeIII GGCC 7 cut(s) 272, 507, 635, 1162, 2177, 2201, 2740
HapII CCGG 6 cut(s) 41, 269, 370, 1827, 2174, 2605
HgaI GACGC 2 cut(s) 1136, 1932
HhaI GCGC 2 cut(s) 2791, 2881
Hin6I GCGC 2 cut(s) 2789, 2879
HinP1I GCGC 2 cut(s) 2789, 2879
HincII GTYRAC 1 cut(s) 134
HindII GTYRAC 1 cut(s) 134
HindIII AAGCTT 2 cut(s) 1600, 2396
HinfI GANTC 9 cut(s) 143, 227, 760, 1280, 1418, 1534, 1593, 2520, 2764
HpaII CCGG 6 cut(s) 41, 269, 370, 1827, 2174, 2605
HphI GGTGA 8 cut(s) 258, 779, 1460, 1763, 2906, 2918, 2947, 2982
Hpy166II GTNNAC 2 cut(s) 134, 512
Hpy8I GTNNAC 2 cut(s) 134, 512
HpyCH4III ACNGT 4 cut(s) 344, 1086, 2806, 2926
HpyCH4IV ACGT 1 cut(s) 2240
HpyF10VI GCNNNNNNNGC 8 cut(s) 278, 409, 1628, 1901, 2177, 2207, 2675, 2687
HpyF3I CTNAG 9 cut(s) 197, 473, 524, 918, 1089, 1687, 1833, 2757, 2834
HpySE526I ACGT 1 cut(s) 2240
HspAI GCGC 2 cut(s) 2789, 2879
Kpn2I TCCGGA 1 cut(s) 2604
Ksp22I TGATCA 1 cut(s) 2110
LguI GCTCTTC 1 cut(s) 882
Lsp1109I GCAGC 9 cut(s) 265, 1634, 1956, 2194, 2367, 2510, 2623, 2690, 2917
LweI GCATC 7 cut(s) 148, 290, 1609, 1882, 2239, 2305, 2820
MaeI CTAG 9 cut(s) 723, 1158, 1164, 1550, 1632, 2004, 2349, 2642, 2817
MaeII ACGT 1 cut(s) 2240
MaeIII GTNAC 5 cut(s) 167, 926, 955, 2419, 2695
MfeI CAATTG 1 cut(s) 1182
MflI RGATCY 1 cut(s) 1729
MhlI GDGCHC 3 cut(s) 595, 1806, 2787
MlyI GAGTC 2 cut(s) 769, 1412
MmeI TCCRAC 4 cut(s) 534, 1974, 2538, 2714
MroI TCCGGA 1 cut(s) 2604
MroXI GAANNNNTTC 4 cut(s) 680, 876, 1354, 1443
MseI TTAA 9 cut(s) 182, 668, 947, 1004, 1152, 2018, 2333, 2340, 2550
MslI CAYNNNNRTG 2 cut(s) 2118, 2382
MspA1I CMGCKG 2 cut(s) 278, 2761
MspCI CTTAAG 2 cut(s) 1151, 2549
MspI CCGG 6 cut(s) 41, 269, 370, 1827, 2174, 2605
MspR9I CCNGG 8 cut(s) 25, 370, 371, 765, 1828, 2153, 2672, 2959
MunI CAATTG 1 cut(s) 1182
Mva1269I GAATGC 2 cut(s) 80, 2016
MvaI CCWGG 5 cut(s) 25, 765, 2153, 2672, 2959
MvnI CGCG 2 cut(s) 315, 2789
MwoI GCNNNNNNNGC 8 cut(s) 278, 409, 1628, 1901, 2177, 2207, 2675, 2687
NciI CCSGG 3 cut(s) 370, 371, 1828
NcoI CCATGG 1 cut(s) 636
NdeI CATATG 1 cut(s) 376
NheI GCTAGC 1 cut(s) 722
NlaIV GGNNCC 5 cut(s) 273, 430, 542, 741, 1803
NmuCI GTSAC 3 cut(s) 167, 955, 2419
NspI RCATGY 2 cut(s) 1663, 1779
PagI TCATGA 1 cut(s) 1650
PceI AGGCCT 1 cut(s) 1162
PciSI GCTCTTC 1 cut(s) 882
PctI GAATGC 2 cut(s) 80, 2016
PdmI GAANNNNTTC 4 cut(s) 680, 876, 1354, 1443
PfeI GAWTC 7 cut(s) 143, 227, 1280, 1534, 1593, 2520, 2764
PflMI CCANNNNNTGG 1 cut(s) 1343
PleI GAGTC 2 cut(s) 768, 1412
PpsI GAGTC 2 cut(s) 768, 1412
Psp124BI GAGCTC 2 cut(s) 595, 2787
Psp6I CCWGG 5 cut(s) 23, 763, 2151, 2670, 2957
PspFI CCCAGC 1 cut(s) 274
PspGI CCWGG 5 cut(s) 23, 763, 2151, 2670, 2957
PspN4I GGNNCC 5 cut(s) 273, 430, 542, 741, 1803
PsrI GAACNNNNNNTAC 2 cut(s) 1186, 1218
PstI CTGCAG 1 cut(s) 2539
PstNI CAGNNNCTG 1 cut(s) 30
PsuI RGATCY 1 cut(s) 1729
PvuII CAGCTG 2 cut(s) 278, 2761
RsaI GTAC 5 cut(s) 187, 1478, 1638, 2161, 2570
RsaNI GTAC 5 cut(s) 186, 1477, 1637, 2160, 2569
RseI CAYNNNNRTG 2 cut(s) 2118, 2382
SacI GAGCTC 2 cut(s) 595, 2787
SapI GCTCTTC 1 cut(s) 882
SaqAI TTAA 9 cut(s) 182, 668, 947, 1004, 1152, 2018, 2333, 2340, 2550
ScaI AGTACT 1 cut(s) 2161
SchI GAGTC 2 cut(s) 769, 1412
ScrFI CCNGG 8 cut(s) 25, 370, 371, 765, 1828, 2153, 2672, 2959
SduI GDGCHC 3 cut(s) 595, 1806, 2787
SfaNI GCATC 7 cut(s) 148, 290, 1609, 1882, 2239, 2305, 2820
SfcI CTRYAG 1 cut(s) 2535
SinI GGWCC 7 cut(s) 153, 446, 739, 2123, 2149, 2560, 2607
SmaI CCCGGG 1 cut(s) 371
SmiMI CAYNNNNRTG 2 cut(s) 2118, 2382
SmlI CTYRAG 3 cut(s) 854, 1151, 2549
SmoI CTYRAG 3 cut(s) 854, 1151, 2549
SpeI ACTAGT 1 cut(s) 2003
SrfI GCCCGGGC 1 cut(s) 371
SseBI AGGCCT 1 cut(s) 1162
SsiI CCGC 5 cut(s) 1749, 1852, 2062, 2178, 2653
SspI AATATT 3 cut(s) 61, 747, 1959
SspMI CTAG 9 cut(s) 723, 1158, 1164, 1550, 1632, 2004, 2349, 2642, 2817
SstI GAGCTC 2 cut(s) 595, 2787
StuI AGGCCT 1 cut(s) 1162
StyD4I CCNGG 8 cut(s) 23, 368, 369, 763, 1826, 2151, 2670, 2957
StyI CCWWGG 3 cut(s) 636, 1932, 2126
TaaI ACNGT 4 cut(s) 344, 1086, 2806, 2926
TaiI ACGT 1 cut(s) 2243
TatI WGTACW 2 cut(s) 185, 2159
TauI GCSGC 2 cut(s) 2065, 2180
TfiI GAWTC 7 cut(s) 143, 227, 1280, 1534, 1593, 2520, 2764
Tru1I TTAA 9 cut(s) 182, 668, 947, 1004, 1152, 2018, 2333, 2340, 2550
Tru9I TTAA 9 cut(s) 182, 668, 947, 1004, 1152, 2018, 2333, 2340, 2550
TscAI CASTG 2 cut(s) 1126, 2811
TseFI GTSAC 3 cut(s) 167, 955, 2419
TseI GCWGC 9 cut(s) 278, 1622, 1969, 2207, 2355, 2498, 2636, 2678, 2930
Tsp45I GTSAC 3 cut(s) 167, 955, 2419
TspGWI ACGGA 1 cut(s) 1451
TspMI CCCGGG 1 cut(s) 369
TspRI CASTG 2 cut(s) 1126, 2811
Van91I CCANNNNNTGG 1 cut(s) 1343
Vha464I CTTAAG 2 cut(s) 1151, 2549
VpaK11BI GGWCC 7 cut(s) 153, 446, 739, 2123, 2149, 2560, 2607
XagI CCTNNNNNAGG 2 cut(s) 2576, 2777
XapI RAATTY 6 cut(s) 213, 1097, 1451, 1795, 2024, 2263
XbaI TCTAGA 1 cut(s) 1549
XceI RCATGY 2 cut(s) 1663, 1779
XcmI CCANNNNNNNNNTGG 1 cut(s) 2709
XmaI CCCGGG 1 cut(s) 369
XmnI GAANNNNTTC 4 cut(s) 680, 876, 1354, 1443
XspI CTAG 9 cut(s) 723, 1158, 1164, 1550, 1632, 2004, 2349, 2642, 2817
ZrmI AGTACT 1 cut(s) 2161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.