FvH4_5g03330

late embryogenesis abundant

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0018035
Physical Location & Seq
Forward (+)
1990345 .. 1991575
1231 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g03330.t1

Sequence Viewer

Length: 537 bp
ATGTTGGAATCAGAGAGGTTCAAGAAAATCCAGAAGAACAGGAAATGCTTTGCATACATCGGAATTTTCATTGTGTTCCAAATCATAGTCATGACTGTTTTTGGATTAACAGTGATGAAGATCAAAACCCCGAGCGTGAGGCTGAGGTCCGTGACAGTCGACAACGCCTCCAATAGTTCCTTCACTCTCATTGCTGAAGTTGCCGTGAAGAACAAAAATTTCGGGAGCTACAAGTTCGATGCCACCACGGCTAATGTGACATACGGCGGTGTCACGCTGGGAAAAGGTGACATTGCCAAGGCACGCGCCGGTTTGAAAAAGACCAAGAGGTTCAATGTGACCATTGAGGTAAGCTCCAGTGATTCCAGCTTTGCCACTGAGCTGGCTGCAGGGAATGTAACACTGAGCGCTCTTTCGAGGGTGACTGGTAAGGTGTCATTGATGGGGATGATGAAGAAGAGGAAGACAGCAAACATGAACTGCTCGATGATCGTTAATGGTCCAAAGAAGGTTGTTTATGATTTGACTTGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

179

Amino Acids

19.43

Weight (kDa)

10.18

Isoelectric Point (pI)

19.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 67 - 162 2.8e-07 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018035)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g03330
malus_domestica MD06G1093300.v1.1 MD14G1114000.v1.1
rosa_chinensis RchiOBHm_Chr7g0196451
rosa_laevigata RLG00000004014
rosa_multiflora Rmu_co8342145.1_g000001
rosa_roxburghii Rroxscaffold_3G00259310
rosa_rugosa Rorug07G0033500
rosa_samantha Rh7CG166100 Rh7DG159100
rosa_wichuraiana Rw7G013790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 159
AccII CGCG 1 cut(s) 306
AciI CCGC 1 cut(s) 267
AcsI RAATTY 2 cut(s) 63, 217
AcuI CTGAAG 1 cut(s) 216
AfeI AGCGCT 1 cut(s) 409
AgsI TTSAA 3 cut(s) 22, 316, 334
AjuI GAANNNNNNNTTGG 2 cut(s) 164, 196
AluBI AGCT 4 cut(s) 228, 354, 369, 382
AluI AGCT 4 cut(s) 228, 354, 369, 382
Ama87I CYCGRG 1 cut(s) 130
Aor51HI AGCGCT 1 cut(s) 409
ApeKI GCWGC 1 cut(s) 386
ApoI RAATTY 2 cut(s) 63, 217
AspLEI GCGC 2 cut(s) 308, 410
AspS9I GGNCC 2 cut(s) 147, 500
AsuHPI GGTGA 2 cut(s) 299, 433
AvaI CYCGRG 1 cut(s) 130
AvaII GGWCC 2 cut(s) 147, 500
BbsI GAAGAC 1 cut(s) 470
BbvCI CCTCAGC 1 cut(s) 143
BbvI GCAGC 1 cut(s) 373
BccI CCATC 1 cut(s) 436
BceAI ACGGC 3 cut(s) 188, 264, 280
BfmI CTRYAG 1 cut(s) 387
BfoI RGCGCY 1 cut(s) 411
BglI GCCNNNNNGGC 1 cut(s) 248
BisI GCNGC 1 cut(s) 387
BlsI GCNGC 1 cut(s) 388
Bme18I GGWCC 2 cut(s) 147, 500
BmeT110I CYCGRG 1 cut(s) 130
BmgT120I GGNCC 2 cut(s) 147, 500
BmsI GCATC 1 cut(s) 229
BpiI GAAGAC 1 cut(s) 470
BplI GAGNNNNNCTC 2 cut(s) 338, 370
BpmI CTGGAG 1 cut(s) 340
Bpu10I CCTNAGC 1 cut(s) 143
BsaBI GATNNNNATC 1 cut(s) 119
BsaJI CCNNGG 2 cut(s) 246, 297
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bse118I RCCGGY 1 cut(s) 308
Bse1I ACTGG 2 cut(s) 357, 430
Bse3DI GCAATG 2 cut(s) 189, 291
Bse8I GATNNNNATC 1 cut(s) 119
BseDI CCNNGG 2 cut(s) 246, 297
BseGI GGATG 1 cut(s) 453
BseJI GATNNNNATC 1 cut(s) 119
BseMI GCAATG 2 cut(s) 189, 291
BseMII CTCAG 3 cut(s) 134, 369, 395
BseNI ACTGG 2 cut(s) 357, 430
BseXI GCAGC 1 cut(s) 373
BseYI CCCAGC 1 cut(s) 277
Bsh1236I CGCG 1 cut(s) 306
BsiHKCI CYCGRG 1 cut(s) 130
BsiSI CCGG 1 cut(s) 309
BsoBI CYCGRG 1 cut(s) 130
Bsp143I GATC 2 cut(s) 120, 489
BspACI CCGC 1 cut(s) 267
BspCNI CTCAG 3 cut(s) 135, 370, 396
BspFNI CGCG 1 cut(s) 306
BspHI TCATGA 1 cut(s) 90
BspMAI CTGCAG 1 cut(s) 391
BsrDI GCAATG 2 cut(s) 189, 291
BsrFI RCCGGY 1 cut(s) 308
BsrI ACTGG 2 cut(s) 357, 430
BssAI RCCGGY 1 cut(s) 308
BssECI CCNNGG 2 cut(s) 246, 297
BssMI GATC 2 cut(s) 120, 489
BssT1I CCWWGG 1 cut(s) 297
Bst4CI ACNGT 3 cut(s) 97, 112, 157
Bst6I CTCTTC 1 cut(s) 452
BstC8I GCNNGC 2 cut(s) 304, 384
BstDEI CTNAG 3 cut(s) 143, 378, 404
BstDSI CCRYGG 1 cut(s) 246
BstF5I GGATG 1 cut(s) 453
BstFNI CGCG 1 cut(s) 306
BstH2I RGCGCY 1 cut(s) 411
BstHHI GCGC 2 cut(s) 308, 410
BstKTI GATC 2 cut(s) 123, 492
BstMBI GATC 2 cut(s) 120, 489
BstMWI GCNNNNNNNGC 2 cut(s) 200, 248
BstSFI CTRYAG 1 cut(s) 387
BstUI CGCG 1 cut(s) 306
BstV1I GCAGC 1 cut(s) 373
BstV2I GAAGAC 1 cut(s) 470
BstXI CCANNNNNNTGG 1 cut(s) 382
BtgI CCRYGG 1 cut(s) 246
BtsCI GGATG 1 cut(s) 453
BtsIMutI CAGTG 4 cut(s) 117, 364, 375, 401
Cac8I GCNNGC 2 cut(s) 304, 384
CciI TCATGA 1 cut(s) 90
CfoI GCGC 2 cut(s) 308, 410
Cfr10I RCCGGY 1 cut(s) 308
Cfr13I GGNCC 2 cut(s) 147, 500
CviAII CATG 2 cut(s) 91, 475
CviJI RGCY 7 cut(s) 142, 228, 251, 354, 369, 382, 386
CviKI_1 RGCY 7 cut(s) 142, 228, 251, 354, 369, 382, 386
DdeI CTNAG 3 cut(s) 143, 378, 404
DpnI GATC 2 cut(s) 122, 491
DpnII GATC 2 cut(s) 120, 489
Eam1104I CTCTTC 1 cut(s) 452
EarI CTCTTC 1 cut(s) 452
Eco130I CCWWGG 1 cut(s) 297
Eco47I GGWCC 2 cut(s) 147, 500
Eco47III AGCGCT 1 cut(s) 409
Eco57I CTGAAG 1 cut(s) 216
Eco88I CYCGRG 1 cut(s) 130
EcoT14I CCWWGG 1 cut(s) 297
ErhI CCWWGG 1 cut(s) 297
FaeI CATG 2 cut(s) 94, 478
FaiI YATR 6 cut(s) 55, 86, 92, 262, 476, 519
FatI CATG 2 cut(s) 90, 474
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 1 cut(s) 387
FokI GGATG 1 cut(s) 460
Fsp4HI GCNGC 1 cut(s) 387
GlaI GCGC 2 cut(s) 307, 409
GluI GCNGC 1 cut(s) 387
GsaI CCCAGC 1 cut(s) 281
GsuI CTGGAG 1 cut(s) 340
HaeII RGCGCY 1 cut(s) 411
HapII CCGG 1 cut(s) 309
HhaI GCGC 2 cut(s) 308, 410
Hin1II CATG 2 cut(s) 94, 478
Hin6I GCGC 2 cut(s) 306, 408
HinP1I GCGC 2 cut(s) 306, 408
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HinfI GANTC 2 cut(s) 8, 362
HpaII CCGG 1 cut(s) 309
HphI GGTGA 2 cut(s) 299, 433
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 2 cut(s) 13, 62
Hpy188III TCNNGA 4 cut(s) 22, 31, 91, 223
Hpy8I GTNNAC 1 cut(s) 160
HpyAV CCTTC 2 cut(s) 190, 502
HpyCH4III ACNGT 3 cut(s) 97, 112, 157
HpyCH4V TGCA 2 cut(s) 53, 389
HpyF10VI GCNNNNNNNGC 2 cut(s) 200, 248
HpyF3I CTNAG 3 cut(s) 143, 378, 404
Hsp92II CATG 2 cut(s) 94, 478
HspAI GCGC 2 cut(s) 306, 408
Kzo9I GATC 2 cut(s) 120, 489
LmnI GCTCC 2 cut(s) 225, 359
LpnPI CCDG 9 cut(s) 25, 44, 263, 322, 368, 370, 375, 379, 411
Lsp1109I GCAGC 1 cut(s) 373
LweI GCATC 1 cut(s) 229
MaeIII GTNAC 7 cut(s) 151, 256, 271, 287, 337, 397, 421
MalI GATC 2 cut(s) 122, 491
MboI GATC 2 cut(s) 120, 489
MboII GAAGA 6 cut(s) 46, 130, 220, 466, 469, 475
MluCI AATT 2 cut(s) 63, 217
MnlI CCTC 8 cut(s) 9, 132, 138, 178, 321, 340, 411, 453
MseI TTAA 2 cut(s) 107, 495
MslI CAYNNNNRTG 1 cut(s) 89
MspI CCGG 1 cut(s) 309
MvnI CGCG 1 cut(s) 306
MwoI GCNNNNNNNGC 2 cut(s) 200, 248
NdeII GATC 2 cut(s) 120, 489
NlaIII CATG 2 cut(s) 94, 478
NmuCI GTSAC 6 cut(s) 151, 256, 271, 287, 337, 421
PagI TCATGA 1 cut(s) 90
PfeI GAWTC 2 cut(s) 8, 362
PkrI GCNGC 1 cut(s) 388
PspFI CCCAGC 1 cut(s) 277
PspPI GGNCC 2 cut(s) 147, 500
PstI CTGCAG 1 cut(s) 391
RseI CAYNNNNRTG 1 cut(s) 89
SalI GTCGAC 1 cut(s) 158
SaqAI TTAA 2 cut(s) 107, 495
SatI GCNGC 1 cut(s) 387
Sau3AI GATC 2 cut(s) 120, 489
Sau96I GGNCC 2 cut(s) 147, 500
SfaNI GCATC 1 cut(s) 229
SfcI CTRYAG 1 cut(s) 387
SinI GGWCC 2 cut(s) 147, 500
SmiMI CAYNNNNRTG 1 cut(s) 89
Sse9I AATT 2 cut(s) 63, 217
SsiI CCGC 1 cut(s) 267
StyI CCWWGG 1 cut(s) 297
TaaI ACNGT 3 cut(s) 97, 112, 157
TaqI TCGA 4 cut(s) 159, 237, 416, 485
TasI AATT 2 cut(s) 63, 217
TfiI GAWTC 2 cut(s) 8, 362
Tru1I TTAA 2 cut(s) 107, 495
Tru9I TTAA 2 cut(s) 107, 495
TscAI CASTG 4 cut(s) 117, 364, 382, 408
TseFI GTSAC 6 cut(s) 151, 256, 271, 287, 337, 421
TseI GCWGC 1 cut(s) 386
Tsp45I GTSAC 6 cut(s) 151, 256, 271, 287, 337, 421
TspDTI ATGAA 4 cut(s) 58, 131, 467, 491
TspGWI ACGGA 1 cut(s) 139
TspRI CASTG 4 cut(s) 117, 364, 382, 408
VpaK11BI GGWCC 2 cut(s) 147, 500
XapI RAATTY 2 cut(s) 63, 217
XmiI GTMKAC 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.