MD14G1114000.v1.1

late embryogenesis abundant

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0018035
Physical Location & Seq
Forward (+)
18400052 .. 18400627
576 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1114000.v1.1.491

Sequence Viewer

Length: 576 bp
ATGTTGGAGTCTGAGAGGTTCAAGAAAATTCAGCAGAACAGGAAATGTTTTGCCTACATCGCTATCTTTGTTGTGTTCCAAATCATAGTCCTGGCCATATTCGGAGCAACCGTGATGAAAATTAAAACTCCGAATGTCCGGCTGAGATCAGTGACGGTCCAAAGCCTAGATTACAGTTCTTCAGGTAGCAATTCTCCATTCTTCAACATGACTTTGATTGCTGAAGTTTCTGTGAGGAACAAGAATTTTGGTCACTTCAAATTTGATCCCACAAAAGCTAATGTCACATTTGGGGGCGTCATAGTGGGGCAAGGTGATATAATCAAAGCAAGAGCCAAGGCTAGGAAGACGAAGAGAATGAACGTGACGATTGAAGTGAACTCTGATGGGATATCGGACGGTGCTAAGCTTGGGACTGAGGTGAGCTCAGGGAATGTGACATTGAGCACTCTTGCTAGGGTTAGAGGTAAGGTGACATTGATGAAGATGATGAAGAAGCGCAAAACAGCACAGATGAATTGCTCAATGATTGTTAATTTGCCAAACCGGCTTGTTTACGATTTGGCTTGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

192

Amino Acids

21.19

Weight (kDa)

10.41

Isoelectric Point (pI)

28.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 76 - 175 7.9e-08 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018035)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g03330
malus_domestica MD06G1093300.v1.1 MD14G1114000.v1.1
rosa_chinensis RchiOBHm_Chr7g0196451
rosa_laevigata RLG00000004014
rosa_multiflora Rmu_co8342145.1_g000001
rosa_roxburghii Rroxscaffold_3G00259310
rosa_rugosa Rorug07G0033500
rosa_samantha Rh7CG166100 Rh7DG159100
rosa_wichuraiana Rw7G013790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 260
AcoI YGGCCR 1 cut(s) 93
AcsI RAATTY 3 cut(s) 27, 244, 260
AcuI CTGAAG 2 cut(s) 165, 243
AcyI GRCGYC 1 cut(s) 297
AfiI CCNNNNNNNGG 1 cut(s) 342
AgsI TTSAA 4 cut(s) 22, 205, 259, 374
AjnI CCWGG 1 cut(s) 90
AluBI AGCT 3 cut(s) 278, 409, 426
AluI AGCT 3 cut(s) 278, 409, 426
Alw21I GWGCWC 2 cut(s) 428, 449
AlwI GGATC 1 cut(s) 260
AoxI GGCC 1 cut(s) 93
ApoI RAATTY 3 cut(s) 27, 244, 260
AspLEI GCGC 1 cut(s) 501
AspS9I GGNCC 1 cut(s) 157
AsuHPI GGTGA 3 cut(s) 326, 433, 484
AvaII GGWCC 1 cut(s) 157
BalI TGGCCA 1 cut(s) 95
BanII GRGCYC 1 cut(s) 428
BbsI GAAGAC 1 cut(s) 353
Bbv12I GWGCWC 2 cut(s) 428, 449
BccI CCATC 1 cut(s) 380
BciT130I CCWGG 1 cut(s) 92
BfaI CTAG 3 cut(s) 167, 342, 456
BglI GCCNNNNNGGC 1 cut(s) 547
BlpI GCTNAGC 1 cut(s) 405
Bme1390I CCNGG 1 cut(s) 92
Bme18I GGWCC 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 157
BmrFI CCNGG 1 cut(s) 92
BpiI GAAGAC 1 cut(s) 353
BplI GAGNNNNNCTC 2 cut(s) 410, 442
Bpu10I CCTNAGC 1 cut(s) 427
Bpu1102I GCTNAGC 1 cut(s) 405
BsaHI GRCGYC 1 cut(s) 297
BsaJI CCNNGG 1 cut(s) 336
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 1 cut(s) 342
Bse118I RCCGGY 1 cut(s) 546
BseBI CCWGG 1 cut(s) 92
BseDI CCNNGG 1 cut(s) 336
BseLI CCNNNNNNNGG 1 cut(s) 342
BseMII CTCAG 3 cut(s) 134, 408, 441
BshFI GGCC 1 cut(s) 95
BsiHKAI GWGCWC 2 cut(s) 428, 449
BsiSI CCGG 2 cut(s) 139, 547
BslFI GGGAC 1 cut(s) 427
BslI CCNNNNNNNGG 1 cut(s) 342
BsmFI GGGAC 1 cut(s) 427
BsnI GGCC 1 cut(s) 95
Bsp1286I GDGCHC 2 cut(s) 428, 449
Bsp143I GATC 2 cut(s) 146, 265
Bsp1720I GCTNAGC 1 cut(s) 405
BspANI GGCC 1 cut(s) 95
BspCNI CTCAG 4 cut(s) 4, 135, 409, 440
BspPI GGATC 1 cut(s) 260
BsrFI RCCGGY 1 cut(s) 546
BssAI RCCGGY 1 cut(s) 546
BssECI CCNNGG 1 cut(s) 336
BssMI GATC 2 cut(s) 146, 265
BssNI GRCGYC 1 cut(s) 297
BssT1I CCWWGG 1 cut(s) 336
Bst2UI CCWGG 1 cut(s) 92
Bst4CI ACNGT 4 cut(s) 112, 157, 176, 401
Bst6I CTCTTC 1 cut(s) 347
BstACI GRCGYC 1 cut(s) 297
BstDEI CTNAG 5 cut(s) 12, 143, 405, 417, 427
BstHHI GCGC 1 cut(s) 501
BstKTI GATC 2 cut(s) 149, 268
BstMBI GATC 2 cut(s) 146, 265
BstMWI GCNNNNNNNGC 2 cut(s) 59, 547
BstNI CCWGG 1 cut(s) 92
BstSCI CCNGG 1 cut(s) 90
BstV2I GAAGAC 1 cut(s) 353
BsuRI GGCC 1 cut(s) 95
BtgZI GCGATG 1 cut(s) 43
BtsIMutI CAGTG 1 cut(s) 156
CfoI GCGC 1 cut(s) 501
Cfr10I RCCGGY 1 cut(s) 546
Cfr13I GGNCC 1 cut(s) 157
CseI GACGC 1 cut(s) 286
CviAII CATG 1 cut(s) 208
DdeI CTNAG 5 cut(s) 12, 143, 405, 417, 427
DpnI GATC 2 cut(s) 148, 267
DpnII GATC 2 cut(s) 146, 265
EaeI YGGCCR 1 cut(s) 93
Eam1104I CTCTTC 1 cut(s) 347
EarI CTCTTC 1 cut(s) 347
Ecl136II GAGCTC 1 cut(s) 426
Eco130I CCWWGG 1 cut(s) 336
Eco24I GRGCYC 1 cut(s) 428
Eco32I GATATC 1 cut(s) 393
Eco47I GGWCC 1 cut(s) 157
Eco53kI GAGCTC 1 cut(s) 426
Eco57I CTGAAG 2 cut(s) 165, 243
EcoICRI GAGCTC 1 cut(s) 426
EcoRII CCWGG 1 cut(s) 90
EcoRV GATATC 1 cut(s) 393
EcoT14I CCWWGG 1 cut(s) 336
EcoT38I GRGCYC 1 cut(s) 428
ErhI CCWWGG 1 cut(s) 336
FaeI CATG 1 cut(s) 211
FaiI YATR 5 cut(s) 86, 98, 209, 302, 320
FaqI GGGAC 1 cut(s) 427
FatI CATG 1 cut(s) 207
FriOI GRGCYC 1 cut(s) 428
FspBI CTAG 3 cut(s) 167, 342, 456
GlaI GCGC 1 cut(s) 500
HaeIII GGCC 1 cut(s) 95
HapII CCGG 2 cut(s) 139, 547
HgaI GACGC 1 cut(s) 286
HhaI GCGC 1 cut(s) 501
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 1 cut(s) 211
Hin6I GCGC 1 cut(s) 499
HinP1I GCGC 1 cut(s) 499
HindIII AAGCTT 1 cut(s) 407
HinfI GANTC 1 cut(s) 8
HpaII CCGG 2 cut(s) 139, 547
HphI GGTGA 3 cut(s) 326, 433, 484
Hpy166II GTNNAC 2 cut(s) 379, 556
Hpy188I TCNGA 5 cut(s) 13, 104, 132, 385, 397
Hpy188III TCNNGA 1 cut(s) 22
Hpy8I GTNNAC 2 cut(s) 379, 556
HpyCH4III ACNGT 4 cut(s) 112, 157, 176, 401
HpyCH4IV ACGT 1 cut(s) 363
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 547
HpyF3I CTNAG 5 cut(s) 12, 143, 405, 417, 427
HpySE526I ACGT 1 cut(s) 363
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 1 cut(s) 211
HspAI GCGC 1 cut(s) 499
Kzo9I GATC 2 cut(s) 146, 265
LmnI GCTCC 1 cut(s) 104
LpnPI CCDG 7 cut(s) 25, 77, 104, 152, 168, 414, 560
MaeI CTAG 3 cut(s) 167, 342, 456
MaeII ACGT 1 cut(s) 363
MaeIII GTNAC 6 cut(s) 151, 251, 283, 364, 436, 472
MalI GATC 2 cut(s) 148, 267
MboI GATC 2 cut(s) 146, 265
MboII GAAGA 6 cut(s) 171, 193, 358, 364, 496, 505
MhlI GDGCHC 2 cut(s) 428, 449
MlsI TGGCCA 1 cut(s) 95
MluCI AATT 7 cut(s) 27, 120, 190, 244, 260, 517, 535
MluNI TGGCCA 1 cut(s) 95
MlyI GAGTC 1 cut(s) 17
MnlI CCTC 4 cut(s) 9, 228, 412, 458
Mox20I TGGCCA 1 cut(s) 95
MscI TGGCCA 1 cut(s) 95
MseI TTAA 2 cut(s) 123, 534
Msp20I TGGCCA 1 cut(s) 95
MspI CCGG 2 cut(s) 139, 547
MspR9I CCNGG 1 cut(s) 92
MvaI CCWGG 1 cut(s) 92
MwoI GCNNNNNNNGC 2 cut(s) 59, 547
NdeII GATC 2 cut(s) 146, 265
NlaIII CATG 1 cut(s) 211
NmuCI GTSAC 6 cut(s) 151, 251, 283, 364, 436, 472
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
Psp124BI GAGCTC 1 cut(s) 428
Psp6I CCWGG 1 cut(s) 90
PspGI CCWGG 1 cut(s) 90
PspPI GGNCC 1 cut(s) 157
SacI GAGCTC 1 cut(s) 428
SaqAI TTAA 2 cut(s) 123, 534
Sau3AI GATC 2 cut(s) 146, 265
Sau96I GGNCC 1 cut(s) 157
SchI GAGTC 1 cut(s) 17
ScrFI CCNGG 1 cut(s) 92
SduI GDGCHC 2 cut(s) 428, 449
SinI GGWCC 1 cut(s) 157
Sse9I AATT 7 cut(s) 27, 120, 190, 244, 260, 517, 535
SspMI CTAG 3 cut(s) 167, 342, 456
SstI GAGCTC 1 cut(s) 428
StyD4I CCNGG 1 cut(s) 90
StyI CCWWGG 1 cut(s) 336
TaaI ACNGT 4 cut(s) 112, 157, 176, 401
TaiI ACGT 1 cut(s) 366
TasI AATT 7 cut(s) 27, 120, 190, 244, 260, 517, 535
Tru1I TTAA 2 cut(s) 123, 534
Tru9I TTAA 2 cut(s) 123, 534
TscAI CASTG 1 cut(s) 156
TseFI GTSAC 6 cut(s) 151, 251, 283, 364, 436, 472
Tsp45I GTSAC 6 cut(s) 151, 251, 283, 364, 436, 472
TspDTI ATGAA 5 cut(s) 131, 374, 497, 506, 530
TspRI CASTG 1 cut(s) 156
VpaK11BI GGWCC 1 cut(s) 157
XapI RAATTY 3 cut(s) 27, 244, 260
XspI CTAG 3 cut(s) 167, 342, 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.