FvH4_5g08410

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0017303
Physical Location & Seq
Reverse (-)
4880898 .. 4883983
3086 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g08410.t2

Sequence Viewer

Length: 1758 bp
ATGGCACAGTGTCATGGGAAGTTTAAGGCCTTCAAGAACAGCAGATGGCTTGTGTTTGTGTGTGCAATGTGGATTCAGTCCTTTGCAGGAATTGGGTACTTGTTTGGGAGCATATCTCCTGTGATAAAGAGCACTATGGGGTACACCCAGAGGGAGCTGGCAATTCTGGGAGTGGCCAAGGACTGGGGTGATGCTATTGGATTTGTTGCCGGATACTTATCGGAGATTTTACCAAGTTGGGGGCTTTTGGTCATTGGAGCAGTGCTCAACTTTCTTGGATATGGATTGCTTTGGCTTATAGTCTCACAAAAGATTGCTGCTTTGCCTTTGTGGGTGCTTTGCATTGCTATATACGTGGGAACGAATGGAGAGACCTTCTTCAACACAGCAGCCTTGATTTCGTGTGTGCAGAACTTCCCCAAAAGCCGGGGACCTGTTGTGGGGATTTTGAAAGGATACGCCGGTTTAAGTGGTGCAATAATAACTCAGATTTATGCAATGTTCAATTCCCCAAATGAAGCGTCATTGCTTTTTATGATTGCAGTTGGACCATCAATGGTTGTCATTGCTCTAATGTTCATTGTTAGGCCTGTGGGAGGTCACAGACAAGTCAGGCCAGGTGACAAGGCAGGATTCACGTTTACATATAGTGTTTGCCTTGTTCTGGCAGCTTATTTGTTGGGAGTTTTGATACTTGAAGATTTGTTTGACTTGAGCCAAACTTTGATCACATTACTTGCGGCCATTTTAGTCGTTTTCTTGGTGCTTCCGATTATCATCCCCATCATATTGAGTTTCTTCTCTGAACCAAAGCCTTTACTTCAGGAGAGCCTTATTGATGTGCAGAGAGAAGATGAAGGGAACAAATCTGAGAATGAGGTCATTTTTAGTGAGGTGGAAGATGAGAAGGCTTCAGATGTAGATTCTCTTCCGTCATCAGAAAGACAAAAACGAATTTCTCAGCTTCAAGCTAAACTGTTCCAAGCAGCTGCAGATGGAGCAGTGAGGGTCAAGAGGAGAAAAGGTCCCCATAGAGGAGAGAATTTCACCTTGATGCAGGCATTGATAAAGGCCGATTTCTGGCTCATATTTGTTTCTCTATTGCTTGCTGCTGGATCTGGCTTGACAGTTATTGACAATTTGGGTCAGATAGTTTTGTCACTTGGGTACACTGATACAAGTATCTATGTCTCCATGATCAGCATTTGGAACTTCCTCGGACGTGTTGGTGGTGGCTACTTCTCTGAGATTATCGTAAGAGACTATGCCTACCCAAGACCAGTGGTAATGGCTGTAGTTCAGGTTATCATGGCAGTTGGGCTTTTCTACTATGCTGTGGGGTTGCCGGGGCAAATATATGTGTCCACTGTGCTGATAGGACTAGGGTATGGTGCTCACTGGGCAATTTTGCCAGCTGCAGCTTCAGAGCTGTTTGGCTTGAAGAGTTTCGGGGCCCTCTATAATTTTCTTACAATGGCAAACCCTGCTGGTTCTCTTATATTCTCAGAAGTGATTGCTAGTGGTATATATGACTACTATGCAGAAAAACAAGCTACACTCAGACACCAAAATCCAAGCTCAATGCTTCTTAAACCCCTCGGGGATGATGATGACCTCTCTTGTGAAGGTACTATATGTTACTCCATCACTTTTGGAATCCTATCAGCACTATGCATTGTTGCAGCAGGATTAAGCATGATTGTAGTTTATCGAACGAGGAGGGTTTACACCCAACTCTATGGGAATCCGAACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

586

Amino Acids

63.7

Weight (kDa)

6.54

Isoelectric Point (pI)

31.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 1 - 96 2e-18 Nodulin-like
NFD4_C PF23262 189 - 344 3.7e-15 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017303)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g08410
malus_domestica MD06G1141000.v1.1 MD14G1156200.v1.1
prunus_persica Prupe.5G150100_v2.0.a1
pyrus_communis pycom06g13100 pycom14g12980
rosa_chinensis RchiOBHm_Chr7g0190031
rosa_laevigata RLG00000004570
rosa_multiflora Rmu_sc0004886.1_g000004
rosa_roxburghii Rroxscaffold_3G00264940
rosa_rugosa Rorug06G0501900
rosa_samantha Rh7BG109600
rosa_wichuraiana Rw7G009260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 183
AciI CCGC 1 cut(s) 740
AclWI GGATC 1 cut(s) 1123
AcoI YGGCCR 2 cut(s) 174, 741
AcsI RAATTY 2 cut(s) 954, 1042
AcuI CTGAAG 3 cut(s) 806, 897, 1407
AfaI GTAC 4 cut(s) 98, 143, 1169, 1630
AfiI CCNNNNNNNGG 8 cut(s) 183, 239, 426, 440, 596, 664, 1034, 1080
AflIII ACRYGT 1 cut(s) 1222
AgsI TTSAA 7 cut(s) 34, 382, 451, 505, 698, 968, 1441
AjiI CACGTC 1 cut(s) 1223
AjnI CCWGG 1 cut(s) 616
Alw21I GWGCWC 3 cut(s) 134, 267, 1396
Alw26I GTCTC 4 cut(s) 307, 365, 1195, 1254
AlwI GGATC 1 cut(s) 1123
Ama87I CYCGRG 1 cut(s) 1598
AoxI GGCC 7 cut(s) 27, 174, 587, 614, 741, 1071, 1452
ApaI GGGCCC 1 cut(s) 1456
ApeKI GCWGC 9 cut(s) 317, 389, 668, 986, 989, 1109, 1415, 1418, 1682
ApoI RAATTY 2 cut(s) 954, 1042
ArsI GACNNNNNNTTYG 2 cut(s) 506, 538
Asp700I GAANNNNTTC 1 cut(s) 1445
AspS9I GGNCC 5 cut(s) 431, 548, 1025, 1452, 1453
AsuC2I CCSGG 2 cut(s) 428, 1347
AsuHPI GGTGA 3 cut(s) 200, 632, 1039
AvaI CYCGRG 1 cut(s) 1598
AvaII GGWCC 3 cut(s) 431, 548, 1025
BaeGI GKGCMC 1 cut(s) 1456
BaeI ACNNNNGTAYC 2 cut(s) 448, 481
BalI TGGCCA 1 cut(s) 176
BanII GRGCYC 1 cut(s) 1456
BarI GAAGNNNNNNTAC 2 cut(s) 912, 944
Bbv12I GWGCWC 3 cut(s) 134, 267, 1396
BbvI GCAGC 9 cut(s) 304, 401, 680, 976, 998, 1096, 1402, 1430, 1694
BccI CCATC 5 cut(s) 39, 559, 791, 989, 1652
BciT130I CCWGG 1 cut(s) 618
BciVI GTATCC 2 cut(s) 206, 449
BclI TGATCA 2 cut(s) 726, 1197
BcnI CCSGG 2 cut(s) 428, 1347
BcoDI GTCTC 4 cut(s) 307, 365, 1195, 1254
BfaI CTAG 2 cut(s) 1382, 1518
BfmI CTRYAG 3 cut(s) 990, 1293, 1416
BfuI GTATCC 2 cut(s) 206, 449
Bme1390I CCNGG 3 cut(s) 428, 618, 1347
Bme18I GGWCC 3 cut(s) 431, 548, 1025
BmeT110I CYCGRG 1 cut(s) 1598
BmgBI CACGTC 1 cut(s) 1223
BmgT120I GGNCC 5 cut(s) 431, 548, 1025, 1452, 1453
BmiI GGNNCC 4 cut(s) 432, 1027, 1453, 1454
BmrFI CCNGG 3 cut(s) 428, 618, 1347
BmrI ACTGGG 2 cut(s) 193, 1408
BmsI GCATC 2 cut(s) 181, 1044
BmuI ACTGGG 2 cut(s) 193, 1408
BplI GAGNNNNNCTC 4 cut(s) 100, 132, 249, 281
BpuEI CTTGAG 1 cut(s) 733
BpuMI CCSGG 2 cut(s) 428, 1347
BsaAI YACGTR 1 cut(s) 355
BsaBI GATNNNNATC 2 cut(s) 217, 776
BsaI GGTCTC 1 cut(s) 365
BsaJI CCNNGG 5 cut(s) 177, 427, 1216, 1346, 1597
BsaXI ACNNNNNCTCC 2 cut(s) 1009, 1039
Bsc4I CCNNNNNNNGG 8 cut(s) 183, 239, 426, 440, 596, 664, 1034, 1080
Bse118I RCCGGY 1 cut(s) 461
Bse1I ACTGG 3 cut(s) 188, 1280, 1403
Bse3DI GCAATG 5 cut(s) 72, 342, 504, 524, 564
Bse8I GATNNNNATC 2 cut(s) 217, 776
BseBI CCWGG 1 cut(s) 618
BseDI CCNNGG 5 cut(s) 177, 427, 1216, 1346, 1597
BseGI GGATG 2 cut(s) 777, 1609
BseJI GATNNNNATC 2 cut(s) 217, 776
BseLI CCNNNNNNNGG 8 cut(s) 183, 239, 426, 440, 596, 664, 1034, 1080
BseMI GCAATG 5 cut(s) 72, 342, 504, 524, 564
BseMII CTCAG 6 cut(s) 500, 861, 974, 1236, 1518, 1573
BseNI ACTGG 3 cut(s) 188, 1280, 1403
BseRI GAGGAG 3 cut(s) 1030, 1050, 1732
BseSI GKGCMC 1 cut(s) 1456
BseXI GCAGC 9 cut(s) 304, 401, 680, 976, 998, 1096, 1402, 1430, 1694
BsgI GTGCAG 2 cut(s) 428, 863
BshFI GGCC 7 cut(s) 29, 176, 589, 616, 743, 1073, 1454
BsiHKAI GWGCWC 3 cut(s) 134, 267, 1396
BsiHKCI CYCGRG 1 cut(s) 1598
BsiSI CCGG 4 cut(s) 210, 427, 462, 1346
BslFI GGGAC 2 cut(s) 444, 1011
BslI CCNNNNNNNGG 8 cut(s) 183, 239, 426, 440, 596, 664, 1034, 1080
BsmAI GTCTC 4 cut(s) 307, 365, 1195, 1254
BsmFI GGGAC 2 cut(s) 444, 1011
BsnI GGCC 7 cut(s) 29, 176, 589, 616, 743, 1073, 1454
Bso31I GGTCTC 1 cut(s) 365
BsoBI CYCGRG 1 cut(s) 1598
Bsp120I GGGCCC 1 cut(s) 1452
Bsp1286I GDGCHC 4 cut(s) 134, 267, 1396, 1456
Bsp143I GATC 3 cut(s) 726, 1115, 1197
BspACI CCGC 1 cut(s) 740
BspANI GGCC 7 cut(s) 29, 176, 589, 616, 743, 1073, 1454
BspCNI CTCAG 6 cut(s) 499, 862, 973, 1237, 1517, 1572
BspLI GGNNCC 4 cut(s) 432, 1027, 1453, 1454
BspMAI CTGCAG 2 cut(s) 994, 1420
BspPI GGATC 1 cut(s) 1123
BspTNI GGTCTC 1 cut(s) 365
BsrDI GCAATG 5 cut(s) 72, 342, 504, 524, 564
BsrFI RCCGGY 1 cut(s) 461
BsrI ACTGG 3 cut(s) 188, 1280, 1403
BssAI RCCGGY 1 cut(s) 461
BssECI CCNNGG 5 cut(s) 177, 427, 1216, 1346, 1597
BssMI GATC 3 cut(s) 726, 1115, 1197
BssT1I CCWWGG 1 cut(s) 177
Bst2UI CCWGG 1 cut(s) 618
Bst4CI ACNGT 4 cut(s) 9, 978, 1129, 1369
Bst6I CTCTTC 2 cut(s) 933, 1436
BstAPI GCANNNNNTGC 1 cut(s) 1484
BstBAI YACGTR 1 cut(s) 355
BstC8I GCNNGC 4 cut(s) 159, 1059, 1107, 1413
BstDEI CTNAG 6 cut(s) 486, 870, 960, 1245, 1504, 1559
BstENI CCTNNNNNAGG 1 cut(s) 594
BstF5I GGATG 2 cut(s) 777, 1609
BstKTI GATC 3 cut(s) 729, 1118, 1200
BstMAI GTCTC 4 cut(s) 307, 365, 1195, 1254
BstMBI GATC 3 cut(s) 726, 1115, 1197
BstMWI GCNNNNNNNGC 3 cut(s) 998, 1400, 1484
BstNI CCWGG 1 cut(s) 618
BstSCI CCNGG 3 cut(s) 426, 616, 1345
BstSFI CTRYAG 3 cut(s) 990, 1293, 1416
BstSLI GKGCMC 1 cut(s) 1456
BstV1I GCAGC 9 cut(s) 304, 401, 680, 976, 998, 1096, 1402, 1430, 1694
BstX2I RGATCY 1 cut(s) 1115
BstXI CCANNNNNNTGG 1 cut(s) 1739
BstYI RGATCY 1 cut(s) 1115
BsuI GTATCC 2 cut(s) 206, 449
BsuRI GGCC 7 cut(s) 29, 176, 589, 616, 743, 1073, 1454
BtrI CACGTC 1 cut(s) 1223
BtsCI GGATG 2 cut(s) 777, 1609
BtsI GCAGTG 2 cut(s) 267, 1008
BtsIMutI CAGTG 7 cut(s) 14, 267, 1008, 1170, 1287, 1365, 1396
Cac8I GCNNGC 4 cut(s) 159, 1059, 1107, 1413
Cfr10I RCCGGY 1 cut(s) 461
Cfr13I GGNCC 5 cut(s) 431, 548, 1025, 1452, 1453
CseI GACGC 1 cut(s) 510
Csp6I GTAC 4 cut(s) 97, 142, 1168, 1629
CspCI CAANNNNNGTGG 2 cut(s) 1263, 1298
CviAII CATG 4 cut(s) 14, 1195, 1309, 1696
CviQI GTAC 4 cut(s) 97, 142, 1168, 1629
DdeI CTNAG 6 cut(s) 486, 870, 960, 1245, 1504, 1559
DpnI GATC 3 cut(s) 728, 1117, 1199
DpnII GATC 3 cut(s) 726, 1115, 1197
EaeI YGGCCR 2 cut(s) 174, 741
Eam1104I CTCTTC 2 cut(s) 933, 1436
EarI CTCTTC 2 cut(s) 933, 1436
Eco130I CCWWGG 1 cut(s) 177
Eco147I AGGCCT 2 cut(s) 29, 589
Eco24I GRGCYC 1 cut(s) 1456
Eco31I GGTCTC 1 cut(s) 365
Eco47I GGWCC 3 cut(s) 431, 548, 1025
Eco57I CTGAAG 3 cut(s) 806, 897, 1407
Eco88I CYCGRG 1 cut(s) 1598
EcoNI CCTNNNNNAGG 1 cut(s) 594
EcoO109I RGGNCCY 4 cut(s) 431, 1025, 1452, 1453
EcoRII CCWGG 1 cut(s) 616
EcoT14I CCWWGG 1 cut(s) 177
EcoT22I ATGCAT 1 cut(s) 1676
EcoT38I GRGCYC 1 cut(s) 1456
ErhI CCWWGG 1 cut(s) 177
FaeI CATG 4 cut(s) 17, 1198, 1312, 1699
FaqI GGGAC 2 cut(s) 444, 1011
FatI CATG 4 cut(s) 13, 1194, 1308, 1695
FbaI TGATCA 2 cut(s) 726, 1197
FokI GGATG 2 cut(s) 764, 1616
FriOI GRGCYC 1 cut(s) 1456
FspBI CTAG 2 cut(s) 1382, 1518
HaeIII GGCC 7 cut(s) 29, 176, 589, 616, 743, 1073, 1454
HapII CCGG 4 cut(s) 210, 427, 462, 1346
HgaI GACGC 1 cut(s) 510
Hin1II CATG 4 cut(s) 17, 1198, 1312, 1699
HinfI GANTC 5 cut(s) 73, 633, 923, 1656, 1744
HpaII CCGG 4 cut(s) 210, 427, 462, 1346
HphI GGTGA 3 cut(s) 200, 632, 1039
Hpy166II GTNNAC 5 cut(s) 144, 642, 1170, 1365, 1726
Hpy188III TCNNGA 3 cut(s) 34, 824, 1012
Hpy8I GTNNAC 5 cut(s) 144, 642, 1170, 1365, 1726
HpyAV CCTTC 5 cut(s) 40, 385, 851, 901, 1619
HpyCH4III ACNGT 4 cut(s) 9, 978, 1129, 1369
HpyCH4IV ACGT 3 cut(s) 354, 638, 1222
HpyF10VI GCNNNNNNNGC 3 cut(s) 998, 1400, 1484
HpyF3I CTNAG 6 cut(s) 486, 870, 960, 1245, 1504, 1559
HpySE526I ACGT 3 cut(s) 354, 638, 1222
Hsp92II CATG 4 cut(s) 17, 1198, 1312, 1699
Ksp22I TGATCA 2 cut(s) 726, 1197
Kzo9I GATC 3 cut(s) 726, 1115, 1197
LmnI GCTCC 4 cut(s) 108, 154, 257, 998
Lsp1109I GCAGC 9 cut(s) 304, 401, 680, 976, 998, 1096, 1402, 1430, 1694
LweI GCATC 2 cut(s) 181, 1044
MaeI CTAG 2 cut(s) 1382, 1518
MaeII ACGT 3 cut(s) 354, 638, 1222
MaeIII GTNAC 4 cut(s) 599, 620, 1158, 1637
MalI GATC 3 cut(s) 728, 1117, 1199
MboI GATC 3 cut(s) 726, 1115, 1197
MboII GAAGA 7 cut(s) 370, 710, 790, 863, 911, 920, 1453
MfeI CAATTG 1 cut(s) 1753
MflI RGATCY 1 cut(s) 1115
MhlI GDGCHC 4 cut(s) 134, 267, 1396, 1456
MlsI TGGCCA 1 cut(s) 176
MluCI AATT 9 cut(s) 90, 162, 505, 954, 1042, 1138, 1404, 1462, 1753
MluNI TGGCCA 1 cut(s) 176
MmeI TCCRAC 1 cut(s) 526
Mox20I TGGCCA 1 cut(s) 176
Mph1103I ATGCAT 1 cut(s) 1676
MroXI GAANNNNTTC 1 cut(s) 1445
MscI TGGCCA 1 cut(s) 176
MseI TTAA 4 cut(s) 24, 467, 1590, 1691
MslI CAYNNNNRTG 1 cut(s) 1052
Msp20I TGGCCA 1 cut(s) 176
MspA1I CMGCKG 2 cut(s) 989, 1415
MspI CCGG 4 cut(s) 210, 427, 462, 1346
MspR9I CCNGG 3 cut(s) 428, 618, 1347
MunI CAATTG 1 cut(s) 1753
MvaI CCWGG 1 cut(s) 618
MwoI GCNNNNNNNGC 3 cut(s) 998, 1400, 1484
NciI CCSGG 2 cut(s) 428, 1347
NdeII GATC 3 cut(s) 726, 1115, 1197
NlaIII CATG 4 cut(s) 17, 1198, 1312, 1699
NlaIV GGNNCC 4 cut(s) 432, 1027, 1453, 1454
NmuCI GTSAC 3 cut(s) 599, 620, 1158
NsiI ATGCAT 1 cut(s) 1676
PceI AGGCCT 2 cut(s) 29, 589
PdmI GAANNNNTTC 1 cut(s) 1445
PfeI GAWTC 5 cut(s) 73, 633, 923, 1656, 1744
PflMI CCANNNNNTGG 1 cut(s) 183
Ppu21I YACGTR 1 cut(s) 355
PpuMI RGGWCCY 2 cut(s) 431, 1025
Psp5II RGGWCCY 2 cut(s) 431, 1025
Psp6I CCWGG 1 cut(s) 616
PspGI CCWGG 1 cut(s) 616
PspN4I GGNNCC 4 cut(s) 432, 1027, 1453, 1454
PspOMI GGGCCC 1 cut(s) 1452
PspPI GGNCC 5 cut(s) 431, 548, 1025, 1452, 1453
PspPPI RGGWCCY 2 cut(s) 431, 1025
PstI CTGCAG 2 cut(s) 994, 1420
PsuI RGATCY 1 cut(s) 1115
PvuII CAGCTG 2 cut(s) 989, 1415
RsaI GTAC 4 cut(s) 98, 143, 1169, 1630
RsaNI GTAC 4 cut(s) 97, 142, 1168, 1629
RseI CAYNNNNRTG 1 cut(s) 1052
SaqAI TTAA 4 cut(s) 24, 467, 1590, 1691
Sau3AI GATC 3 cut(s) 726, 1115, 1197
Sau96I GGNCC 5 cut(s) 431, 548, 1025, 1452, 1453
ScrFI CCNGG 3 cut(s) 428, 618, 1347
SduI GDGCHC 4 cut(s) 134, 267, 1396, 1456
SfaNI GCATC 2 cut(s) 181, 1044
SfcI CTRYAG 3 cut(s) 990, 1293, 1416
SinI GGWCC 3 cut(s) 431, 548, 1025
SmiMI CAYNNNNRTG 1 cut(s) 1052
SmlI CTYRAG 1 cut(s) 712
SmoI CTYRAG 1 cut(s) 712
Sse9I AATT 9 cut(s) 90, 162, 505, 954, 1042, 1138, 1404, 1462, 1753
SseBI AGGCCT 2 cut(s) 29, 589
SsiI CCGC 1 cut(s) 740
SspMI CTAG 2 cut(s) 1382, 1518
StuI AGGCCT 2 cut(s) 29, 589
StyD4I CCNGG 3 cut(s) 426, 616, 1345
StyI CCWWGG 1 cut(s) 177
TaaI ACNGT 4 cut(s) 9, 978, 1129, 1369
TaiI ACGT 3 cut(s) 357, 641, 1225
TaqI TCGA 1 cut(s) 1711
TasI AATT 9 cut(s) 90, 162, 505, 954, 1042, 1138, 1404, 1462, 1753
TauI GCSGC 1 cut(s) 743
TfiI GAWTC 5 cut(s) 73, 633, 923, 1656, 1744
Tru1I TTAA 4 cut(s) 24, 467, 1590, 1691
Tru9I TTAA 4 cut(s) 24, 467, 1590, 1691
TscAI CASTG 7 cut(s) 14, 267, 1008, 1177, 1287, 1372, 1403
TseFI GTSAC 3 cut(s) 599, 620, 1158
TseI GCWGC 9 cut(s) 317, 389, 668, 986, 989, 1109, 1415, 1418, 1682
Tsp45I GTSAC 3 cut(s) 599, 620, 1158
TspDTI ATGAA 3 cut(s) 531, 568, 870
TspGWI ACGGA 1 cut(s) 921
TspRI CASTG 7 cut(s) 14, 267, 1008, 1177, 1287, 1372, 1403
Van91I CCANNNNNTGG 1 cut(s) 183
VpaK11BI GGWCC 3 cut(s) 431, 548, 1025
XagI CCTNNNNNAGG 1 cut(s) 594
XapI RAATTY 2 cut(s) 954, 1042
XmnI GAANNNNTTC 1 cut(s) 1445
XspI CTAG 2 cut(s) 1382, 1518
Zsp2I ATGCAT 1 cut(s) 1676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.