MD06G1141000.v1.1

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0017303
Physical Location & Seq
Forward (+)
28482906 .. 28486194
3289 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1141000.v1.1.491

Sequence Viewer

Length: 1776 bp
ATGGTCCTACTCCATGGGAAGTTCAAGGCCTTCGCCAACAGCAGATGGCTGGTGTTCGTGTGTGCAATGTGGATCCAATCCTTTGCAGGAGTAGGGTACCTTTTTGGGAGCATATCACCTGTGATCAAGTCCACCATGGGCTACAGCCAGAGACAGTTGGCAATTCTTGGAGTGGCCAAGGACTTGGGGGACTCTGTAGGGTTTGTGGCTGGAAGCCTGTCTGAAATATTGCCCACCTGGGGACTTTTGGTAATTGGAGCAGCCTTCAACTTTGTTGGGTATGGCTTGCTTTGGCTTATAGTCTCTCAGACTTTGCCTGCTTTCCCTCTCTGGGTGCTCTGCATTTGTATATTTGTGGGAACCAACGGAGAGACCTTCTTCAACACAGCAGCATTGATATCATGTGTGCAAAACTTCCCCAAAAGCCGGGGTCCTATAGTCGGGATCCTCAAAGGCTACGCGGGGTTGAGTGGCGCAATACTGACTCAGATTTATGCAATGATCAATGCTCCGAATGAATCCTCGTTGATTTTTATGATTGCAGTTGGTCCATCGATGGTTGCCGTTGCTCTGATGTTCATTGTTAGGCCTGTGGGAGGTCACAGGCAAGTGAGGCCATCTGACAATTCAAGCTTCCTATTTACTTACAGCGTTTGCCTTGTTCTGGCAGCTTATATGTTTGGAGTTTTGCTCCTTGAAGATTTGGTCAACATAAGTCAAACTTTGATCACATTGTGTGCAGTTATCTTGATCGTTCTCATTCTGCTTCCAATTGTGATCCCCGTCATACTGGTTTTCTTTTCCGAGCCAACACATTCTGTGCAGGAGAGCCTTCTCCTCGAATCGCAAAGTGAAGAAGCTGGAAAATCTGAGCAGGACAGGACTGAGGTCATTTTAAGTGAGGTGGAGAGTGAGGTGGAGGATGAGAAGTCTCGGGAAGTGGATTCGCTTCCCATGTCCGAAAGACAGAAAAGAATTGCTCAGCTTCAGGCTAAGCTGTTCCAGGCAGCTGCTGTCGGAGCAGTGAGAGTTAAGAGGCGAAAAGGCCCTCATAGAGGAGAGGATTTTACTTTGTTGCAGGCATTGGTAAAGGCTGATTTCTGGCTCATATTTGTTTCCCTACTGCTGGCTGCTGGTTCTGGTTTGACAATTATTGACAATTTAGGTCAAATAACCGAGTCACTTGGGTACGATGATTCGAGTATTTATGTTTCCATGATCAGCATTTGGAACTTTCTTGGACGCGTTGGGGGCGGTTACTTCTCTGAGATTATAGTAAGAGATTACGCATACCCTAGACCCGTGGCCATGGCGGTAGTTCAGGTCATCATGGCACTCGGGCTTTTCTACTATGCCGTGGGATTGCCAGGGCAGATTTATGTGATTACTGTGCTGGTAGGACTTGGTTATGGTGCTCACTGGGCAATTTTGCCAGCTGCAGCCTCAGAGCTATTCGGGCTGAAGAGTTTCGGGGCCTTGTACAATTTTCTTACAATGGCAAATCCTGCTGGTACTCTTATATTCTCAGAAGTGATTGCCAGTGGAATATACGATCACTACGCAAAGGAACAAGCCGCACTCAGACGCCAAATTTCGGGTTCAATGCTCATAAAACCCCTCCGGGATGACGACTCACTCACTTGTGTTGGCACTGTATGCTATTCCATCACTTGTGGAATACTTTCAGGACTCTGCATTGTTGCTTTTGTCTTAAGTTTAATCGTTATTTATCGGACTAGGAGGGTTTATACACAGCTATATGGATCGAGCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

592

Amino Acids

64.18

Weight (kDa)

6.34

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 8 - 160 6.4e-06 Major Facilitator Superfamily
Nodulin-like PF06813 15 - 262 4.7e-92 Nodulin-like
NFD4_C PF23262 362 - 523 1.3e-14 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017303)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g08410
malus_domestica MD06G1141000.v1.1 MD14G1156200.v1.1
prunus_persica Prupe.5G150100_v2.0.a1
pyrus_communis pycom06g13100 pycom14g12980
rosa_chinensis RchiOBHm_Chr7g0190031
rosa_laevigata RLG00000004570
rosa_multiflora Rmu_sc0004886.1_g000004
rosa_roxburghii Rroxscaffold_3G00264940
rosa_rugosa Rorug06G0501900
rosa_samantha Rh7BG109600
rosa_wichuraiana Rw7G009260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 96
AccB1I GGYRCC 1 cut(s) 96
AccII CGCG 2 cut(s) 461, 1245
AciI CCGC 4 cut(s) 461, 1254, 1313, 1575
AclWI GGATC 6 cut(s) 67, 80, 439, 452, 772, 1771
AcoI YGGCCR 2 cut(s) 174, 1305
AcsI RAATTY 1 cut(s) 1590
AcuI CTGAAG 2 cut(s) 971, 1481
AcyI GRCGYC 1 cut(s) 1585
AdeI CACNNNGTG 1 cut(s) 735
AfaI GTAC 4 cut(s) 98, 1190, 1481, 1513
AfiI CCNNNNNNNGG 9 cut(s) 239, 331, 426, 440, 596, 664, 1055, 1126, 1594
AflII CTTAAG 1 cut(s) 1711
AflIII ACRYGT 1 cut(s) 1243
AgsI TTSAA 6 cut(s) 25, 268, 382, 630, 698, 1602
AjnI CCWGG 3 cut(s) 236, 1002, 1366
AluBI AGCT 9 cut(s) 633, 671, 860, 985, 997, 1010, 1436, 1450, 1756
AluI AGCT 9 cut(s) 633, 671, 860, 985, 997, 1010, 1436, 1450, 1756
Alw21I GWGCWC 2 cut(s) 339, 1417
Alw26I GTCTC 4 cut(s) 145, 307, 365, 936
AlwI GGATC 6 cut(s) 67, 80, 439, 452, 772, 1771
AlwNI CAGNNNCTG 1 cut(s) 1013
Ama87I CYCGRG 2 cut(s) 933, 1337
AoxI GGCC 7 cut(s) 27, 174, 587, 614, 1045, 1305, 1473
ApeKI GCWGC 8 cut(s) 260, 389, 668, 1007, 1010, 1130, 1436, 1439
ApoI RAATTY 1 cut(s) 1590
Asp700I GAANNNNTTC 2 cut(s) 1466, 1681
Asp718I GGTACC 1 cut(s) 96
AspLEI GCGC 1 cut(s) 476
AspS9I GGNCC 5 cut(s) 4, 431, 548, 1046, 1473
AsuC2I CCSGG 2 cut(s) 428, 1622
AsuHPI GGTGA 1 cut(s) 108
AvaI CYCGRG 2 cut(s) 933, 1337
AvaII GGWCC 3 cut(s) 4, 431, 548
BalI TGGCCA 2 cut(s) 176, 1307
BamHI GGATCC 2 cut(s) 72, 444
BanI GGYRCC 1 cut(s) 96
Bbv12I GWGCWC 2 cut(s) 339, 1417
BbvI GCAGC 8 cut(s) 272, 401, 680, 997, 1019, 1117, 1423, 1451
BccI CCATC 5 cut(s) 39, 550, 559, 625, 1673
BceAI ACGGC 2 cut(s) 548, 1340
BciT130I CCWGG 3 cut(s) 238, 1004, 1368
BclI TGATCA 4 cut(s) 123, 501, 726, 1218
BcnI CCSGG 2 cut(s) 428, 1622
BcoDI GTCTC 4 cut(s) 145, 307, 365, 936
BfaI CTAG 2 cut(s) 1296, 1737
BfmI CTRYAG 4 cut(s) 142, 195, 435, 1437
BfrI CTTAAG 1 cut(s) 1711
BisI GCNGC 9 cut(s) 261, 390, 669, 1008, 1011, 1131, 1437, 1440, 1575
BlpI GCTNAGC 2 cut(s) 981, 993
BlsI GCNGC 9 cut(s) 262, 391, 670, 1009, 1012, 1132, 1438, 1441, 1576
Bme1390I CCNGG 5 cut(s) 238, 428, 1004, 1368, 1622
Bme18I GGWCC 3 cut(s) 4, 431, 548
BmeT110I CYCGRG 2 cut(s) 933, 1337
BmgT120I GGNCC 5 cut(s) 4, 431, 548, 1046, 1473
BmiI GGNNCC 6 cut(s) 74, 98, 361, 432, 446, 1474
BmrFI CCNGG 5 cut(s) 238, 428, 1004, 1368, 1622
BmrI ACTGGG 1 cut(s) 1429
BmuI ACTGGG 1 cut(s) 1429
BoxI GACNNNNGTC 1 cut(s) 887
BplI GAGNNNNNCTC 2 cut(s) 675, 707
Bpu1102I GCTNAGC 2 cut(s) 981, 993
BpuMI CCSGG 2 cut(s) 428, 1622
Bsa29I ATCGAT 1 cut(s) 554
BsaHI GRCGYC 1 cut(s) 1585
BsaI GGTCTC 1 cut(s) 365
BsaJI CCNNGG 9 cut(s) 13, 135, 177, 237, 427, 1302, 1308, 1356, 1367
Bsc4I CCNNNNNNNGG 9 cut(s) 239, 331, 426, 440, 596, 664, 1055, 1126, 1594
Bse1I ACTGG 3 cut(s) 795, 1424, 1539
Bse3DI GCAATG 2 cut(s) 72, 504
BseBI CCWGG 3 cut(s) 238, 1004, 1368
BseCI ATCGAT 1 cut(s) 554
BseDI CCNNGG 9 cut(s) 13, 135, 177, 237, 427, 1302, 1308, 1356, 1367
BseGI GGATG 2 cut(s) 928, 1630
BseLI CCNNNNNNNGG 9 cut(s) 239, 331, 426, 440, 596, 664, 1055, 1126, 1594
BseMI GCAATG 2 cut(s) 72, 504
BseMII CTCAG 9 cut(s) 320, 500, 861, 876, 995, 1257, 1458, 1539, 1594
BseNI ACTGG 3 cut(s) 795, 1424, 1539
BseRI GAGGAG 2 cut(s) 827, 1071
BseXI GCAGC 8 cut(s) 272, 401, 680, 997, 1019, 1117, 1423, 1451
BsgI GTGCAG 2 cut(s) 759, 842
Bsh1236I CGCG 2 cut(s) 461, 1245
BshFI GGCC 7 cut(s) 29, 176, 589, 616, 1047, 1307, 1475
BshNI GGYRCC 1 cut(s) 96
BshVI ATCGAT 1 cut(s) 554
BsiHKAI GWGCWC 2 cut(s) 339, 1417
BsiHKCI CYCGRG 2 cut(s) 933, 1337
BsiSI CCGG 2 cut(s) 427, 1621
BslFI GGGAC 2 cut(s) 203, 255
BslI CCNNNNNNNGG 9 cut(s) 239, 331, 426, 440, 596, 664, 1055, 1126, 1594
BsmAI GTCTC 4 cut(s) 145, 307, 365, 936
BsmFI GGGAC 2 cut(s) 203, 255
BsnI GGCC 7 cut(s) 29, 176, 589, 616, 1047, 1307, 1475
Bso31I GGTCTC 1 cut(s) 365
BsoBI CYCGRG 2 cut(s) 933, 1337
Bsp1286I GDGCHC 2 cut(s) 339, 1417
Bsp1407I TGTACA 1 cut(s) 1479
Bsp1720I GCTNAGC 2 cut(s) 981, 993
Bsp19I CCATGG 3 cut(s) 13, 135, 1308
BspACI CCGC 4 cut(s) 461, 1254, 1313, 1575
BspANI GGCC 7 cut(s) 29, 176, 589, 616, 1047, 1307, 1475
BspCNI CTCAG 9 cut(s) 319, 499, 862, 877, 994, 1258, 1457, 1538, 1593
BspDI ATCGAT 1 cut(s) 554
BspFNI CGCG 2 cut(s) 461, 1245
BspLI GGNNCC 6 cut(s) 74, 98, 361, 432, 446, 1474
BspMAI CTGCAG 1 cut(s) 1441
BspPI GGATC 6 cut(s) 67, 80, 439, 452, 772, 1771
BspT107I GGYRCC 1 cut(s) 96
BspTI CTTAAG 1 cut(s) 1711
BspTNI GGTCTC 1 cut(s) 365
BsrDI GCAATG 2 cut(s) 72, 504
BsrGI TGTACA 1 cut(s) 1479
BsrI ACTGG 3 cut(s) 795, 1424, 1539
BssECI CCNNGG 9 cut(s) 13, 135, 177, 237, 427, 1302, 1308, 1356, 1367
BssNI GRCGYC 1 cut(s) 1585
BssT1I CCWWGG 4 cut(s) 13, 135, 177, 1308
Bst2UI CCWGG 3 cut(s) 238, 1004, 1368
Bst4CI ACNGT 3 cut(s) 156, 1390, 1654
Bst6I CTCTTC 1 cut(s) 1457
BstACI GRCGYC 1 cut(s) 1585
BstAFI CTTAAG 1 cut(s) 1711
BstAPI GCANNNNNTGC 2 cut(s) 1505, 1656
BstAUI TGTACA 1 cut(s) 1479
BstC8I GCNNGC 5 cut(s) 287, 318, 1080, 1128, 1434
BstDSI CCRYGG 5 cut(s) 13, 135, 1302, 1308, 1356
BstENI CCTNNNNNAGG 2 cut(s) 594, 1053
BstF5I GGATG 2 cut(s) 928, 1630
BstFNI CGCG 2 cut(s) 461, 1245
BstHHI GCGC 1 cut(s) 476
BstMAI GTCTC 4 cut(s) 145, 307, 365, 936
BstMWI GCNNNNNNNGC 7 cut(s) 613, 1019, 1251, 1421, 1456, 1505, 1656
BstNI CCWGG 3 cut(s) 238, 1004, 1368
BstPAI GACNNNNGTC 1 cut(s) 887
BstSCI CCNGG 5 cut(s) 236, 426, 1002, 1366, 1620
BstSFI CTRYAG 4 cut(s) 142, 195, 435, 1437
BstUI CGCG 2 cut(s) 461, 1245
BstV1I GCAGC 8 cut(s) 272, 401, 680, 997, 1019, 1117, 1423, 1451
BstX2I RGATCY 2 cut(s) 72, 444
BstXI CCANNNNNNTGG 1 cut(s) 184
BstYI RGATCY 2 cut(s) 72, 444
Bsu15I ATCGAT 1 cut(s) 554
BsuRI GGCC 7 cut(s) 29, 176, 589, 616, 1047, 1307, 1475
BsuTUI ATCGAT 1 cut(s) 554
BtgI CCRYGG 5 cut(s) 13, 135, 1302, 1308, 1356
BtsCI GGATG 2 cut(s) 928, 1630
BtsI GCAGTG 1 cut(s) 1029
BtsIMutI CAGTG 4 cut(s) 1029, 1417, 1546, 1650
Cac8I GCNNGC 5 cut(s) 287, 318, 1080, 1128, 1434
CaiI CAGNNNCTG 1 cut(s) 1013
CfoI GCGC 1 cut(s) 476
Cfr13I GGNCC 5 cut(s) 4, 431, 548, 1046, 1473
ClaI ATCGAT 1 cut(s) 554
CseI GACGC 2 cut(s) 1251, 1593
Csp6I GTAC 4 cut(s) 97, 1189, 1480, 1512
CviAII CATG 7 cut(s) 14, 136, 402, 955, 1216, 1309, 1330
CviQI GTAC 4 cut(s) 97, 1189, 1480, 1512
DraIII CACNNNGTG 1 cut(s) 735
EaeI YGGCCR 2 cut(s) 174, 1305
Eam1104I CTCTTC 1 cut(s) 1457
EarI CTCTTC 1 cut(s) 1457
Eco130I CCWWGG 4 cut(s) 13, 135, 177, 1308
Eco147I AGGCCT 2 cut(s) 29, 589
Eco31I GGTCTC 1 cut(s) 365
Eco32I GATATC 1 cut(s) 399
Eco47I GGWCC 3 cut(s) 4, 431, 548
Eco57I CTGAAG 2 cut(s) 971, 1481
Eco88I CYCGRG 2 cut(s) 933, 1337
EcoNI CCTNNNNNAGG 2 cut(s) 594, 1053
EcoO109I RGGNCCY 3 cut(s) 431, 1046, 1473
EcoRII CCWGG 3 cut(s) 236, 1002, 1366
EcoRV GATATC 1 cut(s) 399
EcoT14I CCWWGG 4 cut(s) 13, 135, 177, 1308
ErhI CCWWGG 4 cut(s) 13, 135, 177, 1308
FaeI CATG 7 cut(s) 17, 139, 405, 958, 1219, 1312, 1333
FalI AAGNNNNNCTT 2 cut(s) 706, 738
FaqI GGGAC 2 cut(s) 203, 255
FatI CATG 7 cut(s) 13, 135, 401, 954, 1215, 1308, 1329
FauI CCCGC 1 cut(s) 454
FbaI TGATCA 4 cut(s) 123, 501, 726, 1218
Fnu4HI GCNGC 9 cut(s) 261, 390, 669, 1008, 1011, 1131, 1437, 1440, 1575
FokI GGATG 2 cut(s) 935, 1637
Fsp4HI GCNGC 9 cut(s) 261, 390, 669, 1008, 1011, 1131, 1437, 1440, 1575
FspBI CTAG 2 cut(s) 1296, 1737
GlaI GCGC 1 cut(s) 475
GluI GCNGC 9 cut(s) 261, 390, 669, 1008, 1011, 1131, 1437, 1440, 1575
HaeIII GGCC 7 cut(s) 29, 176, 589, 616, 1047, 1307, 1475
HapII CCGG 2 cut(s) 427, 1621
HgaI GACGC 2 cut(s) 1251, 1593
HhaI GCGC 1 cut(s) 476
Hin1I GRCGYC 1 cut(s) 1585
Hin1II CATG 7 cut(s) 17, 139, 405, 958, 1219, 1312, 1333
Hin6I GCGC 1 cut(s) 474
HinP1I GCGC 1 cut(s) 474
HincII GTYRAC 1 cut(s) 709
HindII GTYRAC 1 cut(s) 709
HindIII AAGCTT 1 cut(s) 631
HinfI GANTC 9 cut(s) 191, 484, 518, 842, 944, 1178, 1196, 1631, 1689
HpaII CCGG 2 cut(s) 427, 1621
HphI GGTGA 1 cut(s) 108
Hpy166II GTNNAC 2 cut(s) 132, 709
Hpy188III TCNNGA 4 cut(s) 442, 748, 935, 1686
Hpy8I GTNNAC 2 cut(s) 132, 709
HpyAV CCTTC 4 cut(s) 40, 274, 385, 842
HpyCH4III ACNGT 3 cut(s) 156, 1390, 1654
HpyF10VI GCNNNNNNNGC 7 cut(s) 613, 1019, 1251, 1421, 1456, 1505, 1656
Hsp92I GRCGYC 1 cut(s) 1585
Hsp92II CATG 7 cut(s) 17, 139, 405, 958, 1219, 1312, 1333
HspAI GCGC 1 cut(s) 474
KpnI GGTACC 1 cut(s) 100
Ksp22I TGATCA 4 cut(s) 123, 501, 726, 1218
LmnI GCTCC 5 cut(s) 108, 257, 514, 696, 1019
Lsp1109I GCAGC 8 cut(s) 272, 401, 680, 997, 1019, 1117, 1423, 1451
MaeI CTAG 2 cut(s) 1296, 1737
MaeIII GTNAC 3 cut(s) 599, 1179, 1256
MboII GAAGA 4 cut(s) 370, 710, 866, 1474
MfeI CAATTG 1 cut(s) 771
MflI RGATCY 2 cut(s) 72, 444
MhlI GDGCHC 2 cut(s) 339, 1417
MlsI TGGCCA 2 cut(s) 176, 1307
MluI ACGCGT 1 cut(s) 1243
MluNI TGGCCA 2 cut(s) 176, 1307
MlyI GAGTC 5 cut(s) 185, 478, 1187, 1625, 1683
MmeI TCCRAC 1 cut(s) 997
Mox20I TGGCCA 2 cut(s) 176, 1307
MroXI GAANNNNTTC 2 cut(s) 1466, 1681
MscI TGGCCA 2 cut(s) 176, 1307
MseI TTAA 4 cut(s) 896, 1032, 1712, 1718
Msp20I TGGCCA 2 cut(s) 176, 1307
MspA1I CMGCKG 2 cut(s) 1010, 1436
MspCI CTTAAG 1 cut(s) 1711
MspI CCGG 2 cut(s) 427, 1621
MspR9I CCNGG 5 cut(s) 238, 428, 1004, 1368, 1622
MunI CAATTG 1 cut(s) 771
MvaI CCWGG 3 cut(s) 238, 1004, 1368
MvnI CGCG 2 cut(s) 461, 1245
MwoI GCNNNNNNNGC 7 cut(s) 613, 1019, 1251, 1421, 1456, 1505, 1656
NciI CCSGG 2 cut(s) 428, 1622
NcoI CCATGG 3 cut(s) 13, 135, 1308
NlaIII CATG 7 cut(s) 17, 139, 405, 958, 1219, 1312, 1333
NlaIV GGNNCC 6 cut(s) 74, 98, 361, 432, 446, 1474
NmuCI GTSAC 2 cut(s) 599, 1179
PceI AGGCCT 2 cut(s) 29, 589
PdmI GAANNNNTTC 2 cut(s) 1466, 1681
PfeI GAWTC 4 cut(s) 518, 842, 944, 1196
PfoI TCCNGGA 1 cut(s) 1620
PkrI GCNGC 9 cut(s) 262, 391, 670, 1009, 1012, 1132, 1438, 1441, 1576
PleI GAGTC 5 cut(s) 185, 478, 1186, 1625, 1683
PpsI GAGTC 5 cut(s) 185, 478, 1186, 1625, 1683
PpuMI RGGWCCY 1 cut(s) 431
PshAI GACNNNNGTC 1 cut(s) 887
Psp5II RGGWCCY 1 cut(s) 431
Psp6I CCWGG 3 cut(s) 236, 1002, 1366
PspGI CCWGG 3 cut(s) 236, 1002, 1366
PspN4I GGNNCC 6 cut(s) 74, 98, 361, 432, 446, 1474
PspPI GGNCC 5 cut(s) 4, 431, 548, 1046, 1473
PspPPI RGGWCCY 1 cut(s) 431
PstI CTGCAG 1 cut(s) 1441
PstNI CAGNNNCTG 1 cut(s) 1013
PsuI RGATCY 2 cut(s) 72, 444
PvuII CAGCTG 2 cut(s) 1010, 1436
RsaI GTAC 4 cut(s) 98, 1190, 1481, 1513
RsaNI GTAC 4 cut(s) 97, 1189, 1480, 1512
SaqAI TTAA 4 cut(s) 896, 1032, 1712, 1718
SatI GCNGC 9 cut(s) 261, 390, 669, 1008, 1011, 1131, 1437, 1440, 1575
Sau96I GGNCC 5 cut(s) 4, 431, 548, 1046, 1473
SchI GAGTC 5 cut(s) 185, 478, 1187, 1625, 1683
ScrFI CCNGG 5 cut(s) 238, 428, 1004, 1368, 1622
SduI GDGCHC 2 cut(s) 339, 1417
SfcI CTRYAG 4 cut(s) 142, 195, 435, 1437
SinI GGWCC 3 cut(s) 4, 431, 548
SmlI CTYRAG 1 cut(s) 1711
SmoI CTYRAG 1 cut(s) 1711
SseBI AGGCCT 2 cut(s) 29, 589
SsiI CCGC 4 cut(s) 461, 1254, 1313, 1575
SspI AATATT 1 cut(s) 228
SspMI CTAG 2 cut(s) 1296, 1737
StuI AGGCCT 2 cut(s) 29, 589
StyD4I CCNGG 5 cut(s) 236, 426, 1002, 1366, 1620
StyI CCWWGG 4 cut(s) 13, 135, 177, 1308
TaaI ACNGT 3 cut(s) 156, 1390, 1654
TaqI TCGA 4 cut(s) 554, 840, 1199, 1766
TatI WGTACW 1 cut(s) 1479
TauI GCSGC 1 cut(s) 1577
TfiI GAWTC 4 cut(s) 518, 842, 944, 1196
Tru1I TTAA 4 cut(s) 896, 1032, 1712, 1718
Tru9I TTAA 4 cut(s) 896, 1032, 1712, 1718
TscAI CASTG 4 cut(s) 1029, 1424, 1546, 1657
TseFI GTSAC 2 cut(s) 599, 1179
TseI GCWGC 8 cut(s) 260, 389, 668, 1007, 1010, 1130, 1436, 1439
Tsp45I GTSAC 2 cut(s) 599, 1179
TspDTI ATGAA 2 cut(s) 531, 568
TspGWI ACGGA 1 cut(s) 381
TspRI CASTG 4 cut(s) 1029, 1424, 1546, 1657
Vha464I CTTAAG 1 cut(s) 1711
VpaK11BI GGWCC 3 cut(s) 4, 431, 548
XagI CCTNNNNNAGG 2 cut(s) 594, 1053
XapI RAATTY 1 cut(s) 1590
XmnI GAANNNNTTC 2 cut(s) 1466, 1681
XspI CTAG 2 cut(s) 1296, 1737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.