Prupe.5G150100_v2.0.a1

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0017303
Physical Location & Seq
Forward (+)
13637005 .. 13640393
3389 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G150100.3

Sequence Viewer

Length: 1764 bp
ATGGTTCACTTTCATGGGAAATTGAAGTCTTTCTTCAACAGCAGATGGCTTGTGTTTGTGTGCGCAATGTGGATTCAGTCATTTGCAGGAATTGGGTACTTGTTTGGGAGCATATCTCCAGTGATCAAGAGCACCATGGGGTACAACCAGAGGCAGGTGGCCATTCTTGGTGTGGCCAAGGACTGGGGTGACGCTGTTGGGTTTGTGGCAGGAAGCTTGTCTGAGGTTTTGCCCACTTGGGGGCTTCTGTCCATTGGGGCAGCTCTCAATTTTCTTGGCTATGGCTTCCTTTGGCTTATAGTCTCTCAAAGATTGCCTGCTTTTCCTTTGTGGGTGCTTTGCATCTGTATATTTGTGGGAACAAACGGAGAGACCTTCTTCAACACAGCAGCATTGATTTCATGTGTGCAGAACTTTCCCAAAAGCCGGGGTCCTGTTGTGGGGATCCTAAAAGGATATGCCGGGTTGAGTGGTGCAATCATTACTCAGATTTATGCAATGATCAATTCCCCAAATGAATCATCATTGCTTTTCATGATTGCAGTTGGCCCATCTATGGTTGTCATTGCTCTGATGTTCATTGTTAGGCCTGTGGGAGGTCACAGACAAGTCAGGCCAGCTGATAATTCAAGTTTCCTATTTACTTATAGTGTTTGCCTTGTCCTGGCAGCTTATTTATTGGGAGTTTTGATTCTTGAGGATGTGCTTAACTTGAGTCAATCTTTGATCACATTGTTTGCAGTCATTTTGATAATTCTCATATTACTTCCAATCATAATCCCTATCATATTAGTTTTCTTCTCCGAACCAACACCTTCGGCGCAGGAGAGCCTTCTCCTTGAGGCTCAGAGAGAAGAAGATGGCAAATCTGAGCAGGACAAAACTGAGGTGATTCTTAGTGAGGTGGAAGATGAGAAGAGTCCAGAAGTAGATTCACTTCCAGCATCAGAGAGACAAAAACGAATTGCTCAGCTGCAGGCTAAACTGTTCCAAGCAGCTGCAGATGGAGCAGTGAGGGTCAAGAGGAGAAAAGGCCCTCGTAGAGGAGAGGATTTCACATTAATGCAGGCATTGATAAAGGCAGATTTCTGGCTTATATTTGTTTCCCTTTTACTGGCTGCCGGATCTGGTCTGACAATTATTGATAATTTGGGTCAGATAACTCAGTCACTTGGGTATACCGATTCAAGTATATATGTCTCCATGATCAGCATTTGGAACTTTCTTGGACGTGTTGGTGGCGGCTACTTCTCTGAGATTATAGTAAGAGACTTCGCCTATCCTAGACCAGTGGCTATGGCTGTAGTTCAGGTCATCATGGCAATCGGGCTCTTCTACTATGCTTTGGGGTTGCCAGGGCAAATATATGTGACAACTGTGTTGGTAGGGCTAGGGTATGGTGCTCACTGGGCAGTTTTGCCAGCTGCAGCTTCAGAGCTATTTGGCTTGAAAAGTTTTGGGGCCCTATATAATTTTCTTACAATGGCAAATCCTGCTGGTTCTCTTATCTTCTCAGAAGTGATTGCTAGTGGTATATACGACCACTACGCGAAGGAACAAGCTGCACTCAGACACCGAGACTCGGCTTCAATGCTTATAAAACCCCTCAGAGTTGATGACTCACTCAGTTGTGTTGGCAATGTATGCTATTCCATTACTTGTGGAATACTCTCAGGACTTTGCATTGTTGCAGCTGCATTGAGTTTAATTGTTGTTTACCGGACTAGGAGGGTTTATACGCAACTCTATGGATCGCGCGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

588

Amino Acids

63.85

Weight (kDa)

8.26

Isoelectric Point (pI)

37.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017303)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g08410
malus_domestica MD06G1141000.v1.1 MD14G1156200.v1.1
prunus_persica Prupe.5G150100_v2.0.a1
pyrus_communis pycom06g13100 pycom14g12980
rosa_chinensis RchiOBHm_Chr7g0190031
rosa_laevigata RLG00000004570
rosa_multiflora Rmu_sc0004886.1_g000004
rosa_roxburghii Rroxscaffold_3G00264940
rosa_rugosa Rorug06G0501900
rosa_samantha Rh7BG109600
rosa_wichuraiana Rw7G009260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1598
AarI CACCTGC 1 cut(s) 145
Acc16I TGCGCA 1 cut(s) 64
Acc36I ACCTGC 1 cut(s) 145
AccB7I CCANNNNNTGG 1 cut(s) 183
AccI GTMKAC 1 cut(s) 1178
AccII CGCG 3 cut(s) 1550, 1756, 1758
AciI CCGC 2 cut(s) 1242, 1758
AclWI GGATC 4 cut(s) 439, 452, 1132, 1759
AcoI YGGCCR 2 cut(s) 159, 174
AcuI CTGAAG 1 cut(s) 1416
AfaI GTAC 2 cut(s) 98, 143
AflIII ACRYGT 1 cut(s) 1231
AgsI TTSAA 7 cut(s) 25, 37, 382, 630, 1188, 1450, 1590
AjiI CACGTC 1 cut(s) 1232
AjnI CCWGG 2 cut(s) 663, 1354
Alw21I GWGCWC 2 cut(s) 134, 1405
Alw26I GTCTC 6 cut(s) 307, 365, 946, 1204, 1263, 1572
AlwI GGATC 4 cut(s) 439, 452, 1132, 1759
AoxI GGCC 7 cut(s) 159, 174, 547, 587, 614, 1033, 1461
ApaI GGGCCC 1 cut(s) 1465
AseI ATTAAT 1 cut(s) 1061
Asp700I GAANNNNTTC 1 cut(s) 29
AspLEI GCGC 3 cut(s) 65, 823, 1758
AspS9I GGNCC 5 cut(s) 431, 548, 1034, 1461, 1462
AsuC2I CCSGG 2 cut(s) 428, 463
AsuHPI GGTGA 2 cut(s) 200, 901
AvaII GGWCC 1 cut(s) 431
BaeGI GKGCMC 1 cut(s) 1465
BalI TGGCCA 2 cut(s) 161, 176
BamHI GGATCC 1 cut(s) 444
BanII GRGCYC 2 cut(s) 1332, 1465
BarI GAAGNNNNNNTAC 2 cut(s) 921, 953
Bbv12I GWGCWC 2 cut(s) 134, 1405
BccI CCATC 4 cut(s) 39, 559, 854, 998
BciT130I CCWGG 2 cut(s) 665, 1356
BclI TGATCA 4 cut(s) 123, 501, 726, 1206
BcnI CCSGG 2 cut(s) 428, 463
BcoDI GTCTC 6 cut(s) 307, 365, 946, 1204, 1263, 1572
BfaI CTAG 4 cut(s) 1284, 1391, 1527, 1725
BfmI CTRYAG 4 cut(s) 974, 999, 1302, 1425
BfuAI ACCTGC 1 cut(s) 145
BlpI GCTNAGC 1 cut(s) 969
Bme1390I CCNGG 4 cut(s) 428, 463, 665, 1356
Bme18I GGWCC 1 cut(s) 431
BmgBI CACGTC 1 cut(s) 1232
BmgT120I GGNCC 5 cut(s) 431, 548, 1034, 1461, 1462
BmiI GGNNCC 4 cut(s) 432, 446, 1462, 1463
BmrFI CCNGG 4 cut(s) 428, 463, 665, 1356
BmrI ACTGGG 2 cut(s) 193, 1417
BmsI GCATC 2 cut(s) 351, 953
BmuI ACTGGG 2 cut(s) 193, 1417
BplI GAGNNNNNCTC 2 cut(s) 100, 132
BpmI CTGGAG 1 cut(s) 102
Bpu1102I GCTNAGC 1 cut(s) 969
BpuEI CTTGAG 3 cut(s) 716, 733, 860
BpuMI CCSGG 2 cut(s) 428, 463
BsaI GGTCTC 1 cut(s) 365
BsaJI CCNNGG 4 cut(s) 135, 177, 427, 1355
BsaWI WCCGGW 1 cut(s) 1719
Bse1I ACTGG 5 cut(s) 119, 188, 1119, 1289, 1412
Bse3DI GCAATG 5 cut(s) 72, 504, 524, 564, 1645
BseBI CCWGG 2 cut(s) 665, 1356
BseDI CCNNGG 4 cut(s) 135, 177, 427, 1355
BseGI GGATG 1 cut(s) 706
BseMI GCAATG 5 cut(s) 72, 504, 524, 564, 1645
BseNI ACTGG 5 cut(s) 119, 188, 1119, 1289, 1412
BseRI GAGGAG 2 cut(s) 1039, 1059
BseSI GKGCMC 1 cut(s) 1465
BsgI GTGCAG 2 cut(s) 428, 1548
Bsh1236I CGCG 3 cut(s) 1550, 1756, 1758
BshFI GGCC 7 cut(s) 161, 176, 549, 589, 616, 1035, 1463
BsiHKAI GWGCWC 2 cut(s) 134, 1405
BsiSI CCGG 4 cut(s) 427, 462, 1122, 1720
BsmAI GTCTC 6 cut(s) 307, 365, 946, 1204, 1263, 1572
BsnI GGCC 7 cut(s) 161, 176, 549, 589, 616, 1035, 1463
Bso31I GGTCTC 1 cut(s) 365
Bsp120I GGGCCC 1 cut(s) 1461
Bsp1286I GDGCHC 4 cut(s) 134, 1332, 1405, 1465
Bsp143I GATC 7 cut(s) 123, 444, 501, 726, 1124, 1206, 1751
Bsp1720I GCTNAGC 1 cut(s) 969
Bsp19I CCATGG 1 cut(s) 135
BspACI CCGC 2 cut(s) 1242, 1758
BspANI GGCC 7 cut(s) 161, 176, 549, 589, 616, 1035, 1463
BspFNI CGCG 3 cut(s) 1550, 1756, 1758
BspHI TCATGA 1 cut(s) 534
BspLI GGNNCC 4 cut(s) 432, 446, 1462, 1463
BspMAI CTGCAG 3 cut(s) 978, 1003, 1429
BspMI ACCTGC 1 cut(s) 145
BspPI GGATC 4 cut(s) 439, 452, 1132, 1759
BspQI GCTCTTC 1 cut(s) 1337
BspTNI GGTCTC 1 cut(s) 365
BsrDI GCAATG 5 cut(s) 72, 504, 524, 564, 1645
BsrI ACTGG 5 cut(s) 119, 188, 1119, 1289, 1412
BssECI CCNNGG 4 cut(s) 135, 177, 427, 1355
BssMI GATC 7 cut(s) 123, 444, 501, 726, 1124, 1206, 1751
BssNAI GTATAC 1 cut(s) 1179
BssT1I CCWWGG 2 cut(s) 135, 177
Bst1107I GTATAC 1 cut(s) 1179
Bst2UI CCWGG 2 cut(s) 665, 1356
Bst4CI ACNGT 2 cut(s) 987, 1378
Bst6I CTCTTC 2 cut(s) 911, 1337
BstAPI GCANNNNNTGC 2 cut(s) 1493, 1644
BstC8I GCNNGC 5 cut(s) 318, 618, 978, 1068, 1422
BstDSI CCRYGG 1 cut(s) 135
BstENI CCTNNNNNAGG 2 cut(s) 594, 1041
BstF5I GGATG 1 cut(s) 706
BstFNI CGCG 3 cut(s) 1550, 1756, 1758
BstHHI GCGC 3 cut(s) 65, 823, 1758
BstKTI GATC 7 cut(s) 126, 447, 504, 729, 1127, 1209, 1754
BstMAI GTCTC 6 cut(s) 307, 365, 946, 1204, 1263, 1572
BstMBI GATC 7 cut(s) 123, 444, 501, 726, 1124, 1206, 1751
BstMWI GCNNNNNNNGC 4 cut(s) 1007, 1409, 1493, 1644
BstNI CCWGG 2 cut(s) 665, 1356
BstSCI CCNGG 4 cut(s) 426, 461, 663, 1354
BstSFI CTRYAG 4 cut(s) 974, 999, 1302, 1425
BstSLI GKGCMC 1 cut(s) 1465
BstUI CGCG 3 cut(s) 1550, 1756, 1758
BstX2I RGATCY 2 cut(s) 444, 1124
BstYI RGATCY 2 cut(s) 444, 1124
BstZ17I GTATAC 1 cut(s) 1179
BsuRI GGCC 7 cut(s) 161, 176, 549, 589, 616, 1035, 1463
BtgI CCRYGG 1 cut(s) 135
BtrI CACGTC 1 cut(s) 1232
BtsCI GGATG 1 cut(s) 706
BtsI GCAGTG 1 cut(s) 1017
BtsIMutI CAGTG 4 cut(s) 126, 1017, 1296, 1405
BveI ACCTGC 1 cut(s) 145
Cac8I GCNNGC 5 cut(s) 318, 618, 978, 1068, 1422
CciI TCATGA 1 cut(s) 534
CfoI GCGC 3 cut(s) 65, 823, 1758
Cfr13I GGNCC 5 cut(s) 431, 548, 1034, 1461, 1462
CseI GACGC 1 cut(s) 200
Csp6I GTAC 2 cut(s) 97, 142
CviAII CATG 6 cut(s) 14, 136, 402, 535, 1204, 1318
CviQI GTAC 2 cut(s) 97, 142
DpnI GATC 7 cut(s) 125, 446, 503, 728, 1126, 1208, 1753
DpnII GATC 7 cut(s) 123, 444, 501, 726, 1124, 1206, 1751
EaeI YGGCCR 2 cut(s) 159, 174
Eam1104I CTCTTC 2 cut(s) 911, 1337
EarI CTCTTC 2 cut(s) 911, 1337
Eco130I CCWWGG 2 cut(s) 135, 177
Eco147I AGGCCT 1 cut(s) 589
Eco24I GRGCYC 2 cut(s) 1332, 1465
Eco31I GGTCTC 1 cut(s) 365
Eco47I GGWCC 1 cut(s) 431
Eco57I CTGAAG 1 cut(s) 1416
EcoNI CCTNNNNNAGG 2 cut(s) 594, 1041
EcoO109I RGGNCCY 4 cut(s) 431, 1034, 1461, 1462
EcoRII CCWGG 2 cut(s) 663, 1354
EcoT14I CCWWGG 2 cut(s) 135, 177
EcoT38I GRGCYC 2 cut(s) 1332, 1465
ErhI CCWWGG 2 cut(s) 135, 177
FaeI CATG 6 cut(s) 17, 139, 405, 538, 1207, 1321
FalI AAGNNNNNCTT 2 cut(s) 17, 49
FatI CATG 6 cut(s) 13, 135, 401, 534, 1203, 1317
FbaI TGATCA 4 cut(s) 123, 501, 726, 1206
FblI GTMKAC 1 cut(s) 1178
FokI GGATG 1 cut(s) 713
FriOI GRGCYC 2 cut(s) 1332, 1465
FspBI CTAG 4 cut(s) 1284, 1391, 1527, 1725
FspI TGCGCA 1 cut(s) 64
GlaI GCGC 3 cut(s) 64, 822, 1757
GsuI CTGGAG 1 cut(s) 102
HaeIII GGCC 7 cut(s) 161, 176, 549, 589, 616, 1035, 1463
HapII CCGG 4 cut(s) 427, 462, 1122, 1720
HgaI GACGC 1 cut(s) 200
HhaI GCGC 3 cut(s) 65, 823, 1758
Hin1II CATG 6 cut(s) 17, 139, 405, 538, 1207, 1321
Hin6I GCGC 3 cut(s) 63, 821, 1756
HinP1I GCGC 3 cut(s) 63, 821, 1756
HindIII AAGCTT 1 cut(s) 214
HpaII CCGG 4 cut(s) 427, 462, 1122, 1720
HphI GGTGA 2 cut(s) 200, 901
Hpy166II GTNNAC 3 cut(s) 7, 1179, 1717
Hpy188III TCNNGA 6 cut(s) 127, 535, 695, 923, 1021, 1674
Hpy8I GTNNAC 3 cut(s) 7, 1179, 1717
HpyAV CCTTC 4 cut(s) 385, 825, 842, 1546
HpyCH4III ACNGT 2 cut(s) 987, 1378
HpyCH4IV ACGT 1 cut(s) 1231
HpyF10VI GCNNNNNNNGC 4 cut(s) 1007, 1409, 1493, 1644
HpySE526I ACGT 1 cut(s) 1231
Hsp92II CATG 6 cut(s) 17, 139, 405, 538, 1207, 1321
HspAI GCGC 3 cut(s) 63, 821, 1756
Ksp22I TGATCA 4 cut(s) 123, 501, 726, 1206
Kzo9I GATC 7 cut(s) 123, 444, 501, 726, 1124, 1206, 1751
LguI GCTCTTC 1 cut(s) 1337
LmnI GCTCC 2 cut(s) 108, 1007
LweI GCATC 2 cut(s) 351, 953
MaeI CTAG 4 cut(s) 1284, 1391, 1527, 1725
MaeII ACGT 1 cut(s) 1231
MaeIII GTNAC 4 cut(s) 188, 599, 1167, 1369
MalI GATC 7 cut(s) 125, 446, 503, 728, 1126, 1208, 1753
MboI GATC 7 cut(s) 123, 444, 501, 726, 1124, 1206, 1751
MboII GAAGA 9 cut(s) 25, 370, 790, 866, 869, 920, 928, 1324, 1501
MflI RGATCY 2 cut(s) 444, 1124
MhlI GDGCHC 4 cut(s) 134, 1332, 1405, 1465
MlsI TGGCCA 2 cut(s) 161, 176
MluNI TGGCCA 2 cut(s) 161, 176
MlyI GAGTC 4 cut(s) 724, 928, 1574, 1613
Mox20I TGGCCA 2 cut(s) 161, 176
MroXI GAANNNNTTC 1 cut(s) 29
MscI TGGCCA 2 cut(s) 161, 176
MseI TTAA 3 cut(s) 708, 1061, 1706
MslI CAYNNNNRTG 2 cut(s) 12, 1061
Msp20I TGGCCA 2 cut(s) 161, 176
MspA1I CMGCKG 5 cut(s) 620, 973, 998, 1424, 1694
MspI CCGG 4 cut(s) 427, 462, 1122, 1720
MspR9I CCNGG 4 cut(s) 428, 463, 665, 1356
MvaI CCWGG 2 cut(s) 665, 1356
MvnI CGCG 3 cut(s) 1550, 1756, 1758
MwoI GCNNNNNNNGC 4 cut(s) 1007, 1409, 1493, 1644
NciI CCSGG 2 cut(s) 428, 463
NcoI CCATGG 1 cut(s) 135
NdeII GATC 7 cut(s) 123, 444, 501, 726, 1124, 1206, 1751
NlaIII CATG 6 cut(s) 17, 139, 405, 538, 1207, 1321
NlaIV GGNNCC 4 cut(s) 432, 446, 1462, 1463
NmeAIII GCCGAG 1 cut(s) 1562
NmuCI GTSAC 4 cut(s) 188, 599, 1167, 1369
NsbI TGCGCA 1 cut(s) 64
PagI TCATGA 1 cut(s) 534
PaqCI CACCTGC 1 cut(s) 145
PceI AGGCCT 1 cut(s) 589
PciSI GCTCTTC 1 cut(s) 1337
PdmI GAANNNNTTC 1 cut(s) 29
PfeI GAWTC 6 cut(s) 73, 518, 691, 892, 932, 1184
PflMI CCANNNNNTGG 1 cut(s) 183
PleI GAGTC 4 cut(s) 723, 927, 1574, 1613
PpsI GAGTC 4 cut(s) 723, 927, 1574, 1613
PpuMI RGGWCCY 1 cut(s) 431
PshBI ATTAAT 1 cut(s) 1061
PsiI TTATAA 1 cut(s) 1598
Psp5II RGGWCCY 1 cut(s) 431
Psp6I CCWGG 2 cut(s) 663, 1354
PspGI CCWGG 2 cut(s) 663, 1354
PspN4I GGNNCC 4 cut(s) 432, 446, 1462, 1463
PspOMI GGGCCC 1 cut(s) 1461
PspPI GGNCC 5 cut(s) 431, 548, 1034, 1461, 1462
PspPPI RGGWCCY 1 cut(s) 431
PstI CTGCAG 3 cut(s) 978, 1003, 1429
PsuI RGATCY 2 cut(s) 444, 1124
PvuII CAGCTG 5 cut(s) 620, 973, 998, 1424, 1694
RsaI GTAC 2 cut(s) 98, 143
RsaNI GTAC 2 cut(s) 97, 142
RseI CAYNNNNRTG 2 cut(s) 12, 1061
SapI GCTCTTC 1 cut(s) 1337
SaqAI TTAA 3 cut(s) 708, 1061, 1706
Sau3AI GATC 7 cut(s) 123, 444, 501, 726, 1124, 1206, 1751
Sau96I GGNCC 5 cut(s) 431, 548, 1034, 1461, 1462
SchI GAGTC 4 cut(s) 724, 928, 1574, 1613
ScrFI CCNGG 4 cut(s) 428, 463, 665, 1356
SduI GDGCHC 4 cut(s) 134, 1332, 1405, 1465
SfaNI GCATC 2 cut(s) 351, 953
SfcI CTRYAG 4 cut(s) 974, 999, 1302, 1425
SinI GGWCC 1 cut(s) 431
SmiMI CAYNNNNRTG 2 cut(s) 12, 1061
SmlI CTYRAG 3 cut(s) 695, 712, 839
SmoI CTYRAG 3 cut(s) 695, 712, 839
SseBI AGGCCT 1 cut(s) 589
SsiI CCGC 2 cut(s) 1242, 1758
SspMI CTAG 4 cut(s) 1284, 1391, 1527, 1725
StuI AGGCCT 1 cut(s) 589
StyD4I CCNGG 4 cut(s) 426, 461, 663, 1354
StyI CCWWGG 2 cut(s) 135, 177
TaaI ACNGT 2 cut(s) 987, 1378
TaiI ACGT 1 cut(s) 1234
TauI GCSGC 1 cut(s) 1245
TfiI GAWTC 6 cut(s) 73, 518, 691, 892, 932, 1184
Tru1I TTAA 3 cut(s) 708, 1061, 1706
Tru9I TTAA 3 cut(s) 708, 1061, 1706
TscAI CASTG 4 cut(s) 126, 1017, 1296, 1412
TseFI GTSAC 4 cut(s) 188, 599, 1167, 1369
Tsp45I GTSAC 4 cut(s) 188, 599, 1167, 1369
TspDTI ATGAA 4 cut(s) 390, 523, 531, 568
TspGWI ACGGA 1 cut(s) 381
TspRI CASTG 4 cut(s) 126, 1017, 1296, 1412
Van91I CCANNNNNTGG 1 cut(s) 183
VpaK11BI GGWCC 1 cut(s) 431
VspI ATTAAT 1 cut(s) 1061
XagI CCTNNNNNAGG 2 cut(s) 594, 1041
XcmI CCANNNNNNNNNTGG 1 cut(s) 169
XmiI GTMKAC 1 cut(s) 1178
XmnI GAANNNNTTC 1 cut(s) 29
XspI CTAG 4 cut(s) 1284, 1391, 1527, 1725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.