FvH4_5g12600

Partial alpha/beta-hydrolase lipase region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011380
Physical Location & Seq
Forward (+)
7107520 .. 7111669
4150 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g12600.t3

Sequence Viewer

Length: 1347 bp
ATGATGCAGCGGCTCGTTGACAACGCCATCGCTGTCACCAAAGAGTCAGTGAAGACGGTGACGTATGAATCGCTGAACAATATTGTGAGATTGATCAACGGAATTTCGGCTTTGTTGTTAGCGCTTTTGCCTGGGAAGGCTAATGTTCTCGAAGGAATCCATGGCTGGGAGCTCCGGCCTACCTTTCGTGGACCTCGATTCCCTCGCTGGATGGAGAATGGCGTGTCGTCTTTCAATCAACTCATTCATGAGCTTTCTGTGGATTCGGATACCGAAGGTTCTAGCATAGATTATGAGGAAGAATATAGTGATGATGACATGTCTCCTGCTTCACCTTGTTCACAAAGCTCAAGAGTCTCCAGGTCTTCTAGTATTCCGAAGGAGTGGACAGGATGGATGTGGATCAGTTACATATTTTATTGGATTTTGTTTCCTGCAAGGTTCATGCTTGGCCTTCCTGGTCTTCTTTGCCGTTCTTCTAATGAGAGGTCCACGCCACCCTCACCAGGAGGCAGTCATTTTTCGCGGAGGAGTAGTTCTTTGAATAAAGTGCAGTTCCTCAAGGATCACATTATTCAGCGCACTACTGATAGGAGACGTGGAGTCATTGAGGATCTTCATCTTGCGATTGAGATATTCATAGAAGCTGTATTCGATCTTGTTTATAAAGCTGCCCATTTTGTTCTTCACCCATTATATGCTCTTAGAACGCTATTTGGGTTGTTTTCATCAGACAGTAACAGATTTCAAGATACTTATGATAGTGCGTCAGAAGCTACTGTTCCAACAGCTACACTTGGAGATGATTATCCCGCTCCTACAGAAACTACAGATAGGAATACTTCGTTTCAGGCATTGAATACTGATGCTAGAACATGCCAGGATGTCATTACAGAGCTTGGGTACCCATATGAAGCTATTCATGTGATCACTCGTGATGGATATGTTCTTCACTTGGAAAGAATCCCAAGACGTGATTCACGGAAAGCTGTTTACCTACAACACGGAATTCTAGATTCATCTATGGGTTGGGTCTCCAATGGGGTTGTCGGTTCACCTGCATTTGCTGCCTACGATCAAGGATATGATGTCTTCCTTGGGAACTTTCGTGGCTTGGTTTCTAGGGAGCATGCTGACAAAAGCATTTCCTCACAACAGTACTGGCAGTACTCAATCAATGAGCATGGGACTCAGGATATCCCTGCAATGATAGAGAAGATCCACCAAATTAAAACTGCGGAATTGAAGCTTAGTCAACCTGATATCGAGGAAGAAACCAATGAGGATAGAAGAGAAACCCCACAGGCTATCAAGATTGATTTTATTATCACCTGCTGGCTTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydro_lipase

Protein Analysis

449

Amino Acids

50.79

Weight (kDa)

5.36

Isoelectric Point (pI)

56.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydro_lipase PF04083 188 - 242 2.3e-15 Partial alpha/beta-hydrolase lipase region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011380)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18460 AT1G73920 AT1G73920
fragaria_vesca FvH4_5g12600 FvH4_5g12600
malus_domestica MD06G1212200.v1.1 MD14G1222700.v1.1
prunus_persica Prupe.5G218200_v2.0.a1
pyrus_communis pycom06g18940 pycom14g18440
rosa_chinensis RchiOBHm_Chr7g0184731
rosa_laevigata RLG00000004955
rosa_multiflora Rmu_ssc0000289.1_g000019
rosa_roxburghii Rroxscaffold_3G00269390
rosa_rugosa Rorug06G0464800
rosa_samantha Rh7AG078100 Rh7BG069200 Rh7CG070000 Rh7DG069400
rosa_wichuraiana Rw7G005730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 666
AarI CACCTGC 2 cut(s) 1066, 1340
Acc36I ACCTGC 2 cut(s) 1066, 1340
Acc65I GGTACC 1 cut(s) 903
AccB1I GGYRCC 1 cut(s) 903
AccBSI CCGCTC 1 cut(s) 815
AccII CGCG 1 cut(s) 526
AciI CCGC 4 cut(s) 10, 526, 813, 1238
AclWI GGATC 4 cut(s) 410, 573, 621, 1213
AcsI RAATTY 2 cut(s) 102, 1008
AfaI GTAC 3 cut(s) 905, 1160, 1169
AfeI AGCGCT 1 cut(s) 123
AfiI CCNNNNNNNGG 2 cut(s) 166, 506
AflIII ACRYGT 1 cut(s) 318
AgsI TTSAA 5 cut(s) 235, 544, 749, 859, 1246
AhdI GACNNNNNGTC 1 cut(s) 602
AjiI CACGTC 2 cut(s) 599, 974
AjnI CCWGG 5 cut(s) 130, 359, 457, 505, 879
AjuI GAANNNNNNNTTGG 2 cut(s) 961, 993
AloI GAACNNNNNNTCC 2 cut(s) 520, 552
Alw21I GWGCWC 1 cut(s) 174
Alw26I GTCTC 4 cut(s) 327, 361, 589, 1039
AlwI GGATC 4 cut(s) 410, 573, 621, 1213
Aor51HI AGCGCT 1 cut(s) 123
AoxI GGCC 2 cut(s) 176, 451
ApeKI GCWGC 3 cut(s) 7, 671, 1067
ApoI RAATTY 2 cut(s) 102, 1008
Asp700I GAANNNNTTC 1 cut(s) 918
Asp718I GGTACC 1 cut(s) 903
AspLEI GCGC 2 cut(s) 124, 582
AspS9I GGNCC 2 cut(s) 191, 489
AsuHPI GGTGA 7 cut(s) 28, 70, 324, 495, 680, 1047, 1321
AvaII GGWCC 2 cut(s) 191, 489
BanI GGYRCC 1 cut(s) 903
BanII GRGCYC 1 cut(s) 174
BauI CACGAG 1 cut(s) 933
BbsI GAAGAC 4 cut(s) 59, 357, 455, 1084
Bbv12I GWGCWC 1 cut(s) 174
BbvI GCAGC 3 cut(s) 19, 658, 1054
BccI CCATC 4 cut(s) 35, 205, 387, 932
BceAI ACGGC 1 cut(s) 456
BciT130I CCWGG 5 cut(s) 132, 361, 459, 507, 881
BciVI GTATCC 1 cut(s) 262
BclI TGATCA 2 cut(s) 93, 927
BcoDI GTCTC 4 cut(s) 327, 361, 589, 1039
BfaI CTAG 5 cut(s) 282, 369, 870, 1013, 1122
BfmI CTRYAG 2 cut(s) 819, 828
BfoI RGCGCY 1 cut(s) 125
BfuAI ACCTGC 2 cut(s) 1066, 1340
BfuI GTATCC 1 cut(s) 262
BisI GCNGC 4 cut(s) 8, 11, 672, 1068
BlsI GCNGC 4 cut(s) 9, 12, 673, 1069
BmcAI AGTACT 2 cut(s) 1160, 1169
Bme1390I CCNGG 5 cut(s) 132, 361, 459, 507, 881
Bme18I GGWCC 2 cut(s) 191, 489
BmeRI GACNNNNNGTC 1 cut(s) 602
BmgBI CACGTC 2 cut(s) 599, 974
BmgT120I GGNCC 2 cut(s) 191, 489
BmiI GGNNCC 1 cut(s) 905
BmrFI CCNGG 5 cut(s) 132, 361, 459, 507, 881
BmsI GCATC 1 cut(s) 856
BpiI GAAGAC 4 cut(s) 59, 357, 455, 1084
BpmI CTGGAG 1 cut(s) 343
BpuEI CTTGAG 2 cut(s) 334, 545
BsaBI GATNNNNATC 3 cut(s) 401, 618, 807
BsaI GGTCTC 1 cut(s) 1039
BsaJI CCNNGG 3 cut(s) 131, 160, 1096
BsaXI ACNNNNNCTCC 2 cut(s) 520, 550
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 506
Bse1I ACTGG 1 cut(s) 1166
Bse3DI GCAATG 1 cut(s) 1212
Bse8I GATNNNNATC 3 cut(s) 401, 618, 807
BseBI CCWGG 5 cut(s) 132, 361, 459, 507, 881
BseDI CCNNGG 3 cut(s) 131, 160, 1096
BseGI GGATG 4 cut(s) 216, 398, 402, 889
BseJI GATNNNNATC 3 cut(s) 401, 618, 807
BseLI CCNNNNNNNGG 2 cut(s) 166, 506
BseMI GCAATG 1 cut(s) 1212
BseMII CTCAG 1 cut(s) 1205
BseNI ACTGG 1 cut(s) 1166
BseRI GAGGAG 1 cut(s) 544
BseXI GCAGC 3 cut(s) 19, 658, 1054
BseYI CCCAGC 1 cut(s) 165
BsgI GTGCAG 1 cut(s) 572
Bsh1236I CGCG 1 cut(s) 526
BshFI GGCC 2 cut(s) 178, 453
BshNI GGYRCC 1 cut(s) 903
BsiHKAI GWGCWC 1 cut(s) 174
BsiSI CCGG 1 cut(s) 175
BslFI GGGAC 1 cut(s) 1201
BslI CCNNNNNNNGG 2 cut(s) 166, 506
BsmAI GTCTC 4 cut(s) 327, 361, 589, 1039
BsmBI CGTCTC 1 cut(s) 589
BsmFI GGGAC 1 cut(s) 1201
BsnI GGCC 2 cut(s) 178, 453
Bso31I GGTCTC 1 cut(s) 1039
Bsp1286I GDGCHC 1 cut(s) 174
Bsp143I GATC 8 cut(s) 93, 402, 565, 613, 655, 927, 1075, 1218
Bsp19I CCATGG 1 cut(s) 160
BspACI CCGC 4 cut(s) 10, 526, 813, 1238
BspANI GGCC 2 cut(s) 178, 453
BspCNI CTCAG 1 cut(s) 1204
BspFNI CGCG 1 cut(s) 526
BspHI TCATGA 1 cut(s) 247
BspLI GGNNCC 1 cut(s) 905
BspMI ACCTGC 2 cut(s) 1066, 1340
BspPI GGATC 4 cut(s) 410, 573, 621, 1213
BspT107I GGYRCC 1 cut(s) 903
BspTNI GGTCTC 1 cut(s) 1039
BsrBI CCGCTC 1 cut(s) 815
BsrDI GCAATG 1 cut(s) 1212
BsrI ACTGG 1 cut(s) 1166
BssECI CCNNGG 3 cut(s) 131, 160, 1096
BssMI GATC 8 cut(s) 93, 402, 565, 613, 655, 927, 1075, 1218
BssSI CACGAG 1 cut(s) 933
BssT1I CCWWGG 2 cut(s) 160, 1096
Bst2BI CACGAG 1 cut(s) 933
Bst2UI CCWGG 5 cut(s) 132, 361, 459, 507, 881
Bst4CI ACNGT 4 cut(s) 58, 737, 781, 1158
Bst6I CTCTTC 1 cut(s) 1285
BstAPI GCANNNNNTGC 1 cut(s) 1067
BstC8I GCNNGC 2 cut(s) 1131, 1337
BstDEI CTNAG 3 cut(s) 704, 1191, 1250
BstDSI CCRYGG 1 cut(s) 160
BstF5I GGATG 4 cut(s) 216, 398, 402, 889
BstFNI CGCG 1 cut(s) 526
BstH2I RGCGCY 1 cut(s) 125
BstHHI GCGC 2 cut(s) 124, 582
BstKTI GATC 8 cut(s) 96, 405, 568, 616, 658, 930, 1078, 1221
BstMAI GTCTC 4 cut(s) 327, 361, 589, 1039
BstMBI GATC 8 cut(s) 93, 402, 565, 613, 655, 927, 1075, 1218
BstMWI GCNNNNNNNGC 2 cut(s) 773, 1067
BstNI CCWGG 5 cut(s) 132, 361, 459, 507, 881
BstNSI RCATGY 3 cut(s) 322, 879, 1133
BstSCI CCNGG 5 cut(s) 130, 359, 457, 505, 879
BstSFI CTRYAG 2 cut(s) 819, 828
BstUI CGCG 1 cut(s) 526
BstV1I GCAGC 3 cut(s) 19, 658, 1054
BstV2I GAAGAC 4 cut(s) 59, 357, 455, 1084
BstX2I RGATCY 2 cut(s) 613, 1218
BstYI RGATCY 2 cut(s) 613, 1218
BsuI GTATCC 1 cut(s) 262
BsuRI GGCC 2 cut(s) 178, 453
BtgI CCRYGG 1 cut(s) 160
BtgZI GCGATG 1 cut(s) 13
BtrI CACGTC 2 cut(s) 599, 974
BtsCI GGATG 4 cut(s) 216, 398, 402, 889
BtsIMutI CAGTG 1 cut(s) 54
BveI ACCTGC 2 cut(s) 1066, 1340
Cac8I GCNNGC 2 cut(s) 1131, 1337
CciI TCATGA 1 cut(s) 247
CfoI GCGC 2 cut(s) 124, 582
Cfr13I GGNCC 2 cut(s) 191, 489
CseI GACGC 1 cut(s) 756
Csp6I GTAC 3 cut(s) 904, 1159, 1168
CviAII CATG 9 cut(s) 161, 248, 319, 445, 876, 923, 1130, 1184, 1344
CviQI GTAC 3 cut(s) 904, 1159, 1168
DdeI CTNAG 3 cut(s) 704, 1191, 1250
DpnI GATC 8 cut(s) 95, 404, 567, 615, 657, 929, 1077, 1220
DpnII GATC 8 cut(s) 93, 402, 565, 613, 655, 927, 1075, 1218
DriI GACNNNNNGTC 1 cut(s) 602
Eam1104I CTCTTC 1 cut(s) 1285
Eam1105I GACNNNNNGTC 1 cut(s) 602
EarI CTCTTC 1 cut(s) 1285
Ecl136II GAGCTC 1 cut(s) 172
Eco130I CCWWGG 2 cut(s) 160, 1096
Eco24I GRGCYC 1 cut(s) 174
Eco31I GGTCTC 1 cut(s) 1039
Eco32I GATATC 2 cut(s) 1198, 1264
Eco47I GGWCC 2 cut(s) 191, 489
Eco47III AGCGCT 1 cut(s) 123
Eco53kI GAGCTC 1 cut(s) 172
EcoICRI GAGCTC 1 cut(s) 172
EcoRI GAATTC 1 cut(s) 1008
EcoRII CCWGG 5 cut(s) 130, 359, 457, 505, 879
EcoRV GATATC 2 cut(s) 1198, 1264
EcoT14I CCWWGG 2 cut(s) 160, 1096
EcoT38I GRGCYC 1 cut(s) 174
ErhI CCWWGG 2 cut(s) 160, 1096
Esp3I CGTCTC 1 cut(s) 589
FaeI CATG 9 cut(s) 164, 251, 322, 448, 879, 926, 1133, 1187, 1347
FaqI GGGAC 1 cut(s) 1201
FatI CATG 9 cut(s) 160, 247, 318, 444, 875, 922, 1129, 1183, 1343
FauI CCCGC 1 cut(s) 820
FauNDI CATATG 1 cut(s) 910
FbaI TGATCA 2 cut(s) 93, 927
Fnu4HI GCNGC 4 cut(s) 8, 11, 672, 1068
FokI GGATG 4 cut(s) 223, 405, 409, 896
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 4 cut(s) 8, 11, 672, 1068
FspBI CTAG 5 cut(s) 282, 369, 870, 1013, 1122
GlaI GCGC 2 cut(s) 123, 581
GluI GCNGC 4 cut(s) 8, 11, 672, 1068
GsaI CCCAGC 1 cut(s) 169
GsuI CTGGAG 1 cut(s) 343
HaeII RGCGCY 1 cut(s) 125
HaeIII GGCC 2 cut(s) 178, 453
HapII CCGG 1 cut(s) 175
HgaI GACGC 1 cut(s) 756
HhaI GCGC 2 cut(s) 124, 582
Hin1II CATG 9 cut(s) 164, 251, 322, 448, 879, 926, 1133, 1187, 1347
Hin6I GCGC 2 cut(s) 122, 580
HinP1I GCGC 2 cut(s) 122, 580
HincII GTYRAC 2 cut(s) 19, 1256
HindII GTYRAC 2 cut(s) 19, 1256
HindIII AAGCTT 1 cut(s) 1247
HpaII CCGG 1 cut(s) 175
HphI GGTGA 7 cut(s) 28, 70, 324, 495, 680, 1047, 1321
Hpy166II GTNNAC 8 cut(s) 19, 191, 341, 387, 492, 994, 1055, 1256
Hpy188I TCNGA 4 cut(s) 268, 378, 733, 772
Hpy188III TCNNGA 8 cut(s) 149, 248, 351, 749, 935, 1013, 1193, 1312
Hpy8I GTNNAC 8 cut(s) 19, 191, 341, 387, 492, 994, 1055, 1256
HpyAV CCTTC 5 cut(s) 130, 146, 269, 373, 464
HpyCH4III ACNGT 4 cut(s) 58, 737, 781, 1158
HpyCH4IV ACGT 3 cut(s) 62, 598, 973
HpyCH4V TGCA 5 cut(s) 7, 437, 553, 1061, 1205
HpyF10VI GCNNNNNNNGC 2 cut(s) 773, 1067
HpyF3I CTNAG 3 cut(s) 704, 1191, 1250
HpySE526I ACGT 3 cut(s) 62, 598, 973
Hsp92II CATG 9 cut(s) 164, 251, 322, 448, 879, 926, 1133, 1187, 1347
HspAI GCGC 2 cut(s) 122, 580
KpnI GGTACC 1 cut(s) 907
Ksp22I TGATCA 2 cut(s) 93, 927
Kzo9I GATC 8 cut(s) 93, 402, 565, 613, 655, 927, 1075, 1218
LmnI GCTCC 4 cut(s) 169, 177, 820, 1126
Lsp1109I GCAGC 3 cut(s) 19, 658, 1054
LweI GCATC 1 cut(s) 856
MaeI CTAG 5 cut(s) 282, 369, 870, 1013, 1122
MaeII ACGT 3 cut(s) 62, 598, 973
MaeIII GTNAC 4 cut(s) 34, 58, 407, 737
MalI GATC 8 cut(s) 95, 404, 567, 615, 657, 929, 1077, 1220
MbiI CCGCTC 1 cut(s) 815
MboI GATC 8 cut(s) 93, 402, 565, 613, 655, 927, 1075, 1218
MflI RGATCY 2 cut(s) 613, 1218
MhlI GDGCHC 1 cut(s) 174
MluCI AATT 4 cut(s) 102, 1008, 1227, 1241
MlyI GAGTC 4 cut(s) 53, 363, 612, 1183
MmeI TCCRAC 1 cut(s) 809
MroXI GAANNNNTTC 1 cut(s) 918
MseI TTAA 1 cut(s) 1230
MspA1I CMGCKG 1 cut(s) 10
MspI CCGG 1 cut(s) 175
MspR9I CCNGG 5 cut(s) 132, 361, 459, 507, 881
MvaI CCWGG 5 cut(s) 132, 361, 459, 507, 881
MvnI CGCG 1 cut(s) 526
MwoI GCNNNNNNNGC 2 cut(s) 773, 1067
NcoI CCATGG 1 cut(s) 160
NdeI CATATG 1 cut(s) 910
NdeII GATC 8 cut(s) 93, 402, 565, 613, 655, 927, 1075, 1218
NlaIII CATG 9 cut(s) 164, 251, 322, 448, 879, 926, 1133, 1187, 1347
NlaIV GGNNCC 1 cut(s) 905
NmuCI GTSAC 2 cut(s) 34, 58
NspI RCATGY 3 cut(s) 322, 879, 1133
PaeI GCATGC 1 cut(s) 1133
PagI TCATGA 1 cut(s) 247
PaqCI CACCTGC 2 cut(s) 1066, 1340
PciI ACATGT 1 cut(s) 318
PcsI WCGNNNNNNNCGW 1 cut(s) 193
PdmI GAANNNNTTC 1 cut(s) 918
PfeI GAWTC 7 cut(s) 68, 156, 198, 263, 963, 977, 1016
PkrI GCNGC 4 cut(s) 9, 12, 673, 1069
PleI GAGTC 4 cut(s) 52, 362, 611, 1183
PpsI GAGTC 4 cut(s) 52, 362, 611, 1183
PscI ACATGT 1 cut(s) 318
PsiI TTATAA 1 cut(s) 666
Psp124BI GAGCTC 1 cut(s) 174
Psp6I CCWGG 5 cut(s) 130, 359, 457, 505, 879
PspFI CCCAGC 1 cut(s) 165
PspGI CCWGG 5 cut(s) 130, 359, 457, 505, 879
PspN4I GGNNCC 1 cut(s) 905
PspPI GGNCC 2 cut(s) 191, 489
PsuI RGATCY 2 cut(s) 613, 1218
RsaI GTAC 3 cut(s) 905, 1160, 1169
RsaNI GTAC 3 cut(s) 904, 1159, 1168
SacI GAGCTC 1 cut(s) 174
SaqAI TTAA 1 cut(s) 1230
SatI GCNGC 4 cut(s) 8, 11, 672, 1068
Sau3AI GATC 8 cut(s) 93, 402, 565, 613, 655, 927, 1075, 1218
Sau96I GGNCC 2 cut(s) 191, 489
ScaI AGTACT 2 cut(s) 1160, 1169
SchI GAGTC 4 cut(s) 53, 363, 612, 1183
ScrFI CCNGG 5 cut(s) 132, 361, 459, 507, 881
SduI GDGCHC 1 cut(s) 174
SfaNI GCATC 1 cut(s) 856
SfcI CTRYAG 2 cut(s) 819, 828
SinI GGWCC 2 cut(s) 191, 489
SmlI CTYRAG 2 cut(s) 349, 560
SmoI CTYRAG 2 cut(s) 349, 560
SphI GCATGC 1 cut(s) 1133
Sse9I AATT 4 cut(s) 102, 1008, 1227, 1241
SsiI CCGC 4 cut(s) 10, 526, 813, 1238
SspI AATATT 1 cut(s) 82
SspMI CTAG 5 cut(s) 282, 369, 870, 1013, 1122
SstI GAGCTC 1 cut(s) 174
StyD4I CCNGG 5 cut(s) 130, 359, 457, 505, 879
StyI CCWWGG 2 cut(s) 160, 1096
TaaI ACNGT 4 cut(s) 58, 737, 781, 1158
TaiI ACGT 3 cut(s) 65, 601, 976
TaqI TCGA 4 cut(s) 150, 196, 654, 1266
TasI AATT 4 cut(s) 102, 1008, 1227, 1241
TatI WGTACW 2 cut(s) 1158, 1167
TauI GCSGC 1 cut(s) 13
TfiI GAWTC 7 cut(s) 68, 156, 198, 263, 963, 977, 1016
Tru1I TTAA 1 cut(s) 1230
Tru9I TTAA 1 cut(s) 1230
TscAI CASTG 1 cut(s) 54
TseFI GTSAC 2 cut(s) 34, 58
TseI GCWGC 3 cut(s) 7, 671, 1067
Tsp45I GTSAC 2 cut(s) 34, 58
TspDTI ATGAA 9 cut(s) 81, 236, 433, 608, 628, 717, 911, 927, 1008
TspGWI ACGGA 3 cut(s) 114, 997, 1020
TspRI CASTG 1 cut(s) 54
VpaK11BI GGWCC 2 cut(s) 191, 489
XapI RAATTY 2 cut(s) 102, 1008
XbaI TCTAGA 1 cut(s) 1012
XceI RCATGY 3 cut(s) 322, 879, 1133
XmnI GAANNNNTTC 1 cut(s) 918
XspI CTAG 5 cut(s) 282, 369, 870, 1013, 1122
ZrmI AGTACT 2 cut(s) 1160, 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.