Rmu_ssc0000289.1_g000019

Partial alpha/beta-hydrolase lipase region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000289.1
OG0011380
Physical Location & Seq
Forward (+)
94744 .. 98609
3866 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000289.1_g000019.1.cds

Sequence Viewer

Length: 2094 bp
atgcagcgactcgtcgacaacgccatcgctgtcaccaaagagtcagtgaagacgtttacgtatgaatcgctgaacaatattgtgagattcatcaatggagtttcggctctgctgttggcgttattgccaggtaaggctaatatccttgaaggcatacatggctgggagctcaggcctacctttcgtggacctcggtttcctcgctggatggaaaatggggtgtcctctttcaatcaattcatacatgagcttcctgtggattctgattccgagggttcgagcgtgagcatagactatggggaagaatatagtgatgaagacttatgtcctgcatccccatcttctcaaagttcaagagtctccaggtcttatagtatgcctaggcaatggacaggatggatgtgtatcagatacatattttattggattctgtttcctgcaaggttcctgcttggccttccggggcttctttgccgttcttcttataataggaccgcaacaccctcttctgcaccaggaagcagtcagctttcgcacaggactagttctttgaatagagtgcagttcctcaaggatcacattattcagcgcactactgataggagacgtggagtcattgaggatcttcatctggcgattgagatattcatagaagctgtattcgatgttgttcataaagcagctcattttgttctttccccatttgatgctcttcgaacactatttgggtggttttcatcaaacaacaacagagttcaagatactcatggtagtgcttcagatgcttctgttcccacagctacacttggagatgatgatccagttcctacagaaaggaacacttcatttcaggcgctaaatactgatgctcgaacatgccaggatgtcattacagagcttgggtatccatacgaagctattcatgtgatcactagtgatggatatgttcttctcttggaaagaatcccaagacgtgattcacgaaaagctgtttatctacaacatggaattctggattcatctatgggttgggtgtccaatggggttgtcggttcacctgcatttgccgcctatgatcaaggatatgatgtcttcctcgggaactttcgtggcttggtttctagggagcacgttgacaaaagcatttcctcacgacagtactggcagtactcaatcaatgagcatgggactcaggatattccagcaatgatagagaagatccaccaagttaaaactgcagaattgaagcttagtcaacctgatattgaggaagaaaccaatggtgatcaaccttataagctttgtgcaatttgccatagcttgggaggagcagctatattaatgtatgttgtaacacagaggatagaagagaaaccccacagactctcgagattgattttattatcacctgctggcttccatgaagattctacctttatgttttcggtcgtggaatactcctttcttctattggctccccttctgcaaccccttgttcctggcatctatataccaaccaaattctttcgcatgcttctaaacaagttggcacgggacttccataattaccctgctgttggaggattggttcagacactgatgagttattttgtaggtggagatagctcaaattgggttggtgtgttgggcctaccacattataacatgaatgacatgccgggggtatcgttccaagtggctctccatcttgcacaaatgaagcgtgcaaaaaggtttaggatgtttgattatgggagagcttctgctaatatggaggtttatggatctccagagccattagacttgggagagtactatgggctcattgatattccggttgatctggttgctggacgtaaggacaaggtaattaggccatcaatggtaaagaagcattataaggtgatgaagaattcacgtgtaaatgcatcatacaatgagtttgagtatgcacatttggacttcacattttcccaccgtgaagaactcttggcatatgtcatgtcacgcctcttgcttgtggagccgcctccaaaacagtccagcccgaagggtctaaagctaaagaagaaaggacagcaggttaatagttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydro_lipase

Protein Analysis

697

Amino Acids

78.65

Weight (kDa)

7.09

Isoelectric Point (pI)

52.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011380)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18460 AT1G73920 AT1G73920
fragaria_vesca FvH4_5g12600 FvH4_5g12600
malus_domestica MD06G1212200.v1.1 MD14G1222700.v1.1
prunus_persica Prupe.5G218200_v2.0.a1
pyrus_communis pycom06g18940 pycom14g18440
rosa_chinensis RchiOBHm_Chr7g0184731
rosa_laevigata RLG00000004955
rosa_multiflora Rmu_ssc0000289.1_g000019
rosa_roxburghii Rroxscaffold_3G00269390
rosa_rugosa Rorug06G0464800
rosa_samantha Rh7AG078100 Rh7BG069200 Rh7CG070000 Rh7DG069400
rosa_wichuraiana Rw7G005730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 486, 1296, 1662, 1899
AarI CACCTGC 2 cut(s) 1066, 1417
Acc36I ACCTGC 3 cut(s) 1066, 1417, 2071
AccB7I CCANNNNNTGG 1 cut(s) 1321
AccI GTMKAC 1 cut(s) 15
AciI CCGC 3 cut(s) 495, 1068, 2027
AclWI GGATC 5 cut(s) 582, 630, 812, 1213, 1792
AcsI RAATTY 3 cut(s) 1008, 1520, 1912
AcuI CTGAAG 1 cut(s) 762
AcvI CACGTG 1 cut(s) 1919
AfaI GTAC 3 cut(s) 1160, 1169, 1814
AfiI CCNNNNNNNGG 2 cut(s) 1321, 1577
AflIII ACRYGT 1 cut(s) 1918
AgsI TTSAA 6 cut(s) 149, 232, 354, 553, 758, 1246
AhdI GACNNNNNGTC 1 cut(s) 611
AhlI ACTAGT 2 cut(s) 542, 932
AjiI CACGTC 2 cut(s) 608, 974
AjnI CCWGG 5 cut(s) 127, 362, 514, 879, 1498
AjuI GAANNNNNNNTTGG 2 cut(s) 961, 993
AloI GAACNNNNNNTCC 2 cut(s) 1572, 1604
Alw21I GWGCWC 2 cut(s) 171, 1131
Alw26I GTCTC 2 cut(s) 364, 598
AlwI GGATC 5 cut(s) 582, 630, 812, 1213, 1792
AlwNI CAGNNNCTG 1 cut(s) 1597
Ama87I CYCGRG 2 cut(s) 1097, 1387
AoxI GGCC 4 cut(s) 173, 454, 1648, 1874
ApeKI GCWGC 3 cut(s) 4, 680, 1331
ApoI RAATTY 3 cut(s) 1008, 1520, 1912
ArsI GACNNNNNNTTYG 2 cut(s) 532, 564
AseI ATTAAT 1 cut(s) 1340
Asp700I GAANNNNTTC 1 cut(s) 918
AspA2I CCTAGG 1 cut(s) 380
AspLEI GCGC 2 cut(s) 591, 856
AspS9I GGNCC 3 cut(s) 188, 492, 1648
AsuC2I CCSGG 2 cut(s) 462, 1680
AsuHPI GGTGA 5 cut(s) 25, 1047, 1295, 1398, 1915
AsuII TTCGAA 1 cut(s) 715
AvaI CYCGRG 2 cut(s) 1097, 1387
AvaII GGWCC 2 cut(s) 188, 492
AvrII CCTAGG 1 cut(s) 380
BanII GRGCYC 2 cut(s) 171, 1824
BbrPI CACGTG 1 cut(s) 1919
BbsI GAAGAC 3 cut(s) 56, 324, 1084
Bbv12I GWGCWC 2 cut(s) 171, 1131
BbvI GCAGC 3 cut(s) 16, 692, 1343
BccI CCATC 7 cut(s) 32, 202, 346, 390, 932, 1713, 1885
BceAI ACGGC 1 cut(s) 459
BciT130I CCWGG 5 cut(s) 129, 364, 516, 881, 1500
BciVI GTATCC 1 cut(s) 915
BclI TGATCA 3 cut(s) 927, 1075, 1285
BcnI CCSGG 2 cut(s) 462, 1680
BcoDI GTCTC 2 cut(s) 364, 598
BcuI ACTAGT 2 cut(s) 542, 932
BfaI CTAG 4 cut(s) 381, 543, 933, 1122
BfmI CTRYAG 2 cut(s) 828, 1236
BfoI RGCGCY 1 cut(s) 857
BfuAI ACCTGC 3 cut(s) 1066, 1417, 2071
BfuI GTATCC 1 cut(s) 915
BisI GCNGC 5 cut(s) 5, 681, 1068, 1332, 2027
BlnI CCTAGG 1 cut(s) 380
BlsI GCNGC 5 cut(s) 6, 682, 1069, 1333, 2028
BmcAI AGTACT 3 cut(s) 1160, 1169, 1814
Bme1390I CCNGG 7 cut(s) 129, 364, 462, 516, 881, 1500, 1680
Bme18I GGWCC 2 cut(s) 188, 492
BmeRI GACNNNNNGTC 1 cut(s) 611
BmeT110I CYCGRG 2 cut(s) 1097, 1387
BmgBI CACGTC 2 cut(s) 608, 974
BmgT120I GGNCC 3 cut(s) 188, 492, 1648
BmiI GGNNCC 3 cut(s) 446, 1476, 2025
BmrFI CCNGG 7 cut(s) 129, 364, 462, 516, 881, 1500, 1680
BmsI GCATC 6 cut(s) 341, 697, 772, 856, 1512, 1937
BoxI GACNNNNGTC 1 cut(s) 324
BpiI GAAGAC 3 cut(s) 56, 324, 1084
BpmI CTGGAG 2 cut(s) 346, 1773
Bpu10I CCTNAGC 1 cut(s) 170
Bpu14I TTCGAA 1 cut(s) 715
BpuEI CTTGAG 1 cut(s) 554
BpuMI CCSGG 2 cut(s) 462, 1680
BsaAI YACGTR 2 cut(s) 60, 1919
BsaBI GATNNNNATC 3 cut(s) 404, 627, 816
BsaJI CCNNGG 6 cut(s) 191, 270, 380, 461, 1096, 1679
BsaWI WCCGGW 1 cut(s) 1834
BsaXI ACNNNNNCTCC 2 cut(s) 1572, 1602
Bsc4I CCNNNNNNNGG 2 cut(s) 1321, 1577
Bse1I ACTGG 2 cut(s) 821, 1166
Bse3DI GCAATG 2 cut(s) 392, 1212
Bse8I GATNNNNATC 3 cut(s) 404, 627, 816
BseBI CCWGG 5 cut(s) 129, 364, 516, 881, 1500
BseDI CCNNGG 6 cut(s) 191, 270, 380, 461, 1096, 1679
BseGI GGATG 6 cut(s) 213, 332, 401, 405, 889, 1746
BseJI GATNNNNATC 3 cut(s) 404, 627, 816
BseLI CCNNNNNNNGG 2 cut(s) 1321, 1577
BseMI GCAATG 2 cut(s) 392, 1212
BseMII CTCAG 2 cut(s) 184, 1205
BseNI ACTGG 2 cut(s) 821, 1166
BseRI GAGGAG 1 cut(s) 1341
BseXI GCAGC 3 cut(s) 16, 692, 1343
BseYI CCCAGC 1 cut(s) 162
BsgI GTGCAG 2 cut(s) 495, 581
Bsh1285I CGRYCG 1 cut(s) 1449
BshFI GGCC 4 cut(s) 175, 456, 1650, 1876
BsiEI CGRYCG 1 cut(s) 1449
BsiHKAI GWGCWC 2 cut(s) 171, 1131
BsiHKCI CYCGRG 2 cut(s) 1097, 1387
BsiSI CCGG 3 cut(s) 461, 1679, 1835
BslFI GGGAC 2 cut(s) 1201, 1568
BslI CCNNNNNNNGG 2 cut(s) 1321, 1577
BsmAI GTCTC 2 cut(s) 364, 598
BsmBI CGTCTC 1 cut(s) 598
BsmFI GGGAC 2 cut(s) 1201, 1568
BsnI GGCC 4 cut(s) 175, 456, 1650, 1876
BsoBI CYCGRG 2 cut(s) 1097, 1387
Bsp119I TTCGAA 1 cut(s) 715
Bsp1286I GDGCHC 3 cut(s) 171, 1131, 1824
Bsp143I GATC 9 cut(s) 574, 622, 817, 927, 1075, 1218, 1285, 1784, 1840
BspACI CCGC 3 cut(s) 495, 1068, 2027
BspANI GGCC 4 cut(s) 175, 456, 1650, 1876
BspCNI CTCAG 2 cut(s) 183, 1204
BspLI GGNNCC 3 cut(s) 446, 1476, 2025
BspMAI CTGCAG 1 cut(s) 1240
BspMI ACCTGC 3 cut(s) 1066, 1417, 2071
BspPI GGATC 5 cut(s) 582, 630, 812, 1213, 1792
BspQI GCTCTTC 1 cut(s) 717
BspT104I TTCGAA 1 cut(s) 715
BsrDI GCAATG 2 cut(s) 392, 1212
BsrI ACTGG 2 cut(s) 821, 1166
BssECI CCNNGG 6 cut(s) 191, 270, 380, 461, 1096, 1679
BssMI GATC 9 cut(s) 574, 622, 817, 927, 1075, 1218, 1285, 1784, 1840
BssT1I CCWWGG 1 cut(s) 380
Bst2UI CCWGG 5 cut(s) 129, 364, 516, 881, 1500
Bst4CI ACNGT 3 cut(s) 1158, 1979, 2040
Bst6I CTCTTC 3 cut(s) 511, 717, 1362
BstBAI YACGTR 2 cut(s) 60, 1919
BstBI TTCGAA 1 cut(s) 715
BstC8I GCNNGC 3 cut(s) 1414, 1532, 1725
BstDEI CTNAG 3 cut(s) 170, 1191, 1250
BstF5I GGATG 6 cut(s) 213, 332, 401, 405, 889, 1746
BstH2I RGCGCY 1 cut(s) 857
BstHHI GCGC 2 cut(s) 591, 856
BstKTI GATC 9 cut(s) 577, 625, 820, 930, 1078, 1221, 1288, 1787, 1843
BstMAI GTCTC 2 cut(s) 364, 598
BstMBI GATC 9 cut(s) 574, 622, 817, 927, 1075, 1218, 1285, 1784, 1840
BstMCI CGRYCG 1 cut(s) 1449
BstMWI GCNNNNNNNGC 4 cut(s) 159, 782, 1067, 2023
BstNI CCWGG 5 cut(s) 129, 364, 516, 881, 1500
BstNSI RCATGY 3 cut(s) 879, 1534, 1678
BstPAI GACNNNNGTC 1 cut(s) 324
BstSCI CCNGG 7 cut(s) 127, 362, 460, 514, 879, 1498, 1678
BstSFI CTRYAG 2 cut(s) 828, 1236
BstSNI TACGTA 1 cut(s) 60
BstV1I GCAGC 3 cut(s) 16, 692, 1343
BstV2I GAAGAC 3 cut(s) 56, 324, 1084
BstX2I RGATCY 3 cut(s) 622, 1218, 1784
BstYI RGATCY 3 cut(s) 622, 1218, 1784
BsuI GTATCC 1 cut(s) 915
BsuRI GGCC 4 cut(s) 175, 456, 1650, 1876
BtgZI GCGATG 1 cut(s) 10
BtrI CACGTC 2 cut(s) 608, 974
BtsCI GGATG 6 cut(s) 213, 332, 401, 405, 889, 1746
BtsIMutI CAGTG 2 cut(s) 51, 1595
BveI ACCTGC 3 cut(s) 1066, 1417, 2071
Cac8I GCNNGC 3 cut(s) 1414, 1532, 1725
CaiI CAGNNNCTG 1 cut(s) 1597
CfoI GCGC 2 cut(s) 591, 856
Cfr13I GGNCC 3 cut(s) 188, 492, 1648
Csp6I GTAC 3 cut(s) 1159, 1168, 1813
CviQI GTAC 3 cut(s) 1159, 1168, 1813
DdeI CTNAG 3 cut(s) 170, 1191, 1250
DpnI GATC 9 cut(s) 576, 624, 819, 929, 1077, 1220, 1287, 1786, 1842
DpnII GATC 9 cut(s) 574, 622, 817, 927, 1075, 1218, 1285, 1784, 1840
DriI GACNNNNNGTC 1 cut(s) 611
Eam1104I CTCTTC 3 cut(s) 511, 717, 1362
Eam1105I GACNNNNNGTC 1 cut(s) 611
EarI CTCTTC 3 cut(s) 511, 717, 1362
Ecl136II GAGCTC 1 cut(s) 169
Eco105I TACGTA 1 cut(s) 60
Eco130I CCWWGG 1 cut(s) 380
Eco147I AGGCCT 1 cut(s) 175
Eco24I GRGCYC 2 cut(s) 171, 1824
Eco47I GGWCC 2 cut(s) 188, 492
Eco53kI GAGCTC 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 762
Eco72I CACGTG 1 cut(s) 1919
Eco88I CYCGRG 2 cut(s) 1097, 1387
EcoICRI GAGCTC 1 cut(s) 169
EcoRI GAATTC 2 cut(s) 1008, 1912
EcoRII CCWGG 5 cut(s) 127, 362, 514, 879, 1498
EcoT14I CCWWGG 1 cut(s) 380
EcoT22I ATGCAT 1 cut(s) 1930
EcoT38I GRGCYC 2 cut(s) 171, 1824
ErhI CCWWGG 1 cut(s) 380
Esp3I CGTCTC 1 cut(s) 598
FalI AAGNNNNNCTT 2 cut(s) 826, 858
FaqI GGGAC 2 cut(s) 1201, 1568
FauNDI CATATG 1 cut(s) 1996
FbaI TGATCA 3 cut(s) 927, 1075, 1285
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 5 cut(s) 5, 681, 1068, 1332, 2027
FokI GGATG 6 cut(s) 220, 319, 408, 412, 896, 1753
FriOI GRGCYC 2 cut(s) 171, 1824
Fsp4HI GCNGC 5 cut(s) 5, 681, 1068, 1332, 2027
FspBI CTAG 4 cut(s) 381, 543, 933, 1122
GlaI GCGC 2 cut(s) 590, 855
GluI GCNGC 5 cut(s) 5, 681, 1068, 1332, 2027
GsaI CCCAGC 1 cut(s) 166
GsuI CTGGAG 2 cut(s) 346, 1773
HaeII RGCGCY 1 cut(s) 857
HaeIII GGCC 4 cut(s) 175, 456, 1650, 1876
HapII CCGG 3 cut(s) 461, 1679, 1835
HhaI GCGC 2 cut(s) 591, 856
Hin6I GCGC 2 cut(s) 589, 854
HinP1I GCGC 2 cut(s) 589, 854
HincII GTYRAC 3 cut(s) 16, 1135, 1256
HindII GTYRAC 3 cut(s) 16, 1135, 1256
HindIII AAGCTT 2 cut(s) 1247, 1298
HpaII CCGG 3 cut(s) 461, 1679, 1835
HphI GGTGA 5 cut(s) 25, 1047, 1295, 1398, 1915
Hpy166II GTNNAC 6 cut(s) 16, 57, 188, 1055, 1135, 1256
Hpy188I TCNGA 5 cut(s) 265, 271, 410, 781, 1593
Hpy8I GTNNAC 6 cut(s) 16, 57, 188, 1055, 1135, 1256
Hpy99I CGWCG 1 cut(s) 17
HpyAV CCTTC 4 cut(s) 143, 467, 1490, 2044
HpyCH4III ACNGT 3 cut(s) 1158, 1979, 2040
HpyCH4IV ACGT 7 cut(s) 53, 59, 607, 973, 1131, 1855, 1918
HpyF10VI GCNNNNNNNGC 4 cut(s) 159, 782, 1067, 2023
HpyF3I CTNAG 3 cut(s) 170, 1191, 1250
HpySE526I ACGT 7 cut(s) 53, 59, 607, 973, 1131, 1855, 1918
HspAI GCGC 2 cut(s) 589, 854
Ksp22I TGATCA 3 cut(s) 927, 1075, 1285
Kzo9I GATC 9 cut(s) 574, 622, 817, 927, 1075, 1218, 1285, 1784, 1840
LguI GCTCTTC 1 cut(s) 717
LmnI GCTCC 5 cut(s) 166, 1126, 1328, 1480, 2023
Lsp1109I GCAGC 3 cut(s) 16, 692, 1343
LweI GCATC 6 cut(s) 341, 697, 772, 856, 1512, 1937
MaeI CTAG 4 cut(s) 381, 543, 933, 1122
MaeII ACGT 7 cut(s) 53, 59, 607, 973, 1131, 1855, 1918
MaeIII GTNAC 3 cut(s) 31, 1351, 2004
MalI GATC 9 cut(s) 576, 624, 819, 929, 1077, 1220, 1287, 1786, 1842
MboI GATC 9 cut(s) 574, 622, 817, 927, 1075, 1218, 1285, 1784, 1840
MflI RGATCY 3 cut(s) 622, 1218, 1784
MhlI GDGCHC 3 cut(s) 171, 1131, 1824
MluCI AATT 9 cut(s) 236, 1008, 1241, 1308, 1520, 1564, 1630, 1869, 1912
MlyI GAGTC 6 cut(s) 3, 50, 366, 621, 1183, 1377
MmeI TCCRAC 1 cut(s) 1558
Mph1103I ATGCAT 1 cut(s) 1930
MroXI GAANNNNTTC 1 cut(s) 918
MseI TTAA 4 cut(s) 1230, 1340, 2085, 2092
MslI CAYNNNNRTG 1 cut(s) 771
MspI CCGG 3 cut(s) 461, 1679, 1835
MspR9I CCNGG 7 cut(s) 129, 364, 462, 516, 881, 1500, 1680
MvaI CCWGG 5 cut(s) 129, 364, 516, 881, 1500
MwoI GCNNNNNNNGC 4 cut(s) 159, 782, 1067, 2023
NciI CCSGG 2 cut(s) 462, 1680
NdeI CATATG 1 cut(s) 1996
NdeII GATC 9 cut(s) 574, 622, 817, 927, 1075, 1218, 1285, 1784, 1840
NlaIV GGNNCC 3 cut(s) 446, 1476, 2025
NmuCI GTSAC 2 cut(s) 31, 2004
NsiI ATGCAT 1 cut(s) 1930
NspI RCATGY 3 cut(s) 879, 1534, 1678
NspV TTCGAA 1 cut(s) 715
PaeI GCATGC 1 cut(s) 1534
PaeR7I CTCGAG 1 cut(s) 1387
PaqCI CACCTGC 2 cut(s) 1066, 1417
PceI AGGCCT 1 cut(s) 175
PciSI GCTCTTC 1 cut(s) 717
PcsI WCGNNNNNNNCGW 1 cut(s) 979
PdmI GAANNNNTTC 1 cut(s) 918
PfeI GAWTC 9 cut(s) 65, 87, 260, 266, 427, 963, 977, 1016, 1427
PflMI CCANNNNNTGG 1 cut(s) 1321
PkrI GCNGC 5 cut(s) 6, 682, 1069, 1333, 2028
PleI GAGTC 6 cut(s) 3, 49, 365, 620, 1183, 1377
PmaCI CACGTG 1 cut(s) 1919
PmlI CACGTG 1 cut(s) 1919
PpsI GAGTC 6 cut(s) 3, 49, 365, 620, 1183, 1377
Ppu21I YACGTR 2 cut(s) 60, 1919
PshAI GACNNNNGTC 1 cut(s) 324
PshBI ATTAAT 1 cut(s) 1340
PsiI TTATAA 4 cut(s) 486, 1296, 1662, 1899
Psp124BI GAGCTC 1 cut(s) 171
Psp6I CCWGG 5 cut(s) 127, 362, 514, 879, 1498
PspCI CACGTG 1 cut(s) 1919
PspFI CCCAGC 1 cut(s) 162
PspGI CCWGG 5 cut(s) 127, 362, 514, 879, 1498
PspN4I GGNNCC 3 cut(s) 446, 1476, 2025
PspPI GGNCC 3 cut(s) 188, 492, 1648
PstI CTGCAG 1 cut(s) 1240
PstNI CAGNNNCTG 1 cut(s) 1597
PsuI RGATCY 3 cut(s) 622, 1218, 1784
RsaI GTAC 3 cut(s) 1160, 1169, 1814
RsaNI GTAC 3 cut(s) 1159, 1168, 1813
RseI CAYNNNNRTG 1 cut(s) 771
SacI GAGCTC 1 cut(s) 171
SalI GTCGAC 1 cut(s) 14
SapI GCTCTTC 1 cut(s) 717
SaqAI TTAA 4 cut(s) 1230, 1340, 2085, 2092
SatI GCNGC 5 cut(s) 5, 681, 1068, 1332, 2027
Sau3AI GATC 9 cut(s) 574, 622, 817, 927, 1075, 1218, 1285, 1784, 1840
Sau96I GGNCC 3 cut(s) 188, 492, 1648
ScaI AGTACT 3 cut(s) 1160, 1169, 1814
SchI GAGTC 6 cut(s) 3, 50, 366, 621, 1183, 1377
ScrFI CCNGG 7 cut(s) 129, 364, 462, 516, 881, 1500, 1680
SduI GDGCHC 3 cut(s) 171, 1131, 1824
SfaNI GCATC 6 cut(s) 341, 697, 772, 856, 1512, 1937
SfcI CTRYAG 2 cut(s) 828, 1236
Sfr274I CTCGAG 1 cut(s) 1387
SfuI TTCGAA 1 cut(s) 715
SinI GGWCC 2 cut(s) 188, 492
SlaI CTCGAG 1 cut(s) 1387
SmiMI CAYNNNNRTG 1 cut(s) 771
SmlI CTYRAG 2 cut(s) 569, 1387
SmoI CTYRAG 2 cut(s) 569, 1387
SnaBI TACGTA 1 cut(s) 60
SpeI ACTAGT 2 cut(s) 542, 932
SphI GCATGC 1 cut(s) 1534
Sse9I AATT 9 cut(s) 236, 1008, 1241, 1308, 1520, 1564, 1630, 1869, 1912
SseBI AGGCCT 1 cut(s) 175
SsiI CCGC 3 cut(s) 495, 1068, 2027
SspI AATATT 1 cut(s) 79
SspMI CTAG 4 cut(s) 381, 543, 933, 1122
SstI GAGCTC 1 cut(s) 171
StuI AGGCCT 1 cut(s) 175
StyD4I CCNGG 7 cut(s) 127, 362, 460, 514, 879, 1498, 1678
StyI CCWWGG 1 cut(s) 380
TaaI ACNGT 3 cut(s) 1158, 1979, 2040
TaiI ACGT 7 cut(s) 56, 62, 610, 976, 1134, 1858, 1921
TaqI TCGA 6 cut(s) 15, 278, 663, 715, 871, 1388
TaqII GACCGA 1 cut(s) 1435
TasI AATT 9 cut(s) 236, 1008, 1241, 1308, 1520, 1564, 1630, 1869, 1912
TatI WGTACW 3 cut(s) 1158, 1167, 1812
TauI GCSGC 2 cut(s) 1070, 2029
TfiI GAWTC 9 cut(s) 65, 87, 260, 266, 427, 963, 977, 1016, 1427
Tru1I TTAA 4 cut(s) 1230, 1340, 2085, 2092
Tru9I TTAA 4 cut(s) 1230, 1340, 2085, 2092
TscAI CASTG 2 cut(s) 51, 1602
TseFI GTSAC 2 cut(s) 31, 2004
TseI GCWGC 3 cut(s) 4, 680, 1331
Tsp45I GTSAC 2 cut(s) 31, 2004
TspRI CASTG 2 cut(s) 51, 1602
Van91I CCANNNNNTGG 1 cut(s) 1321
VpaK11BI GGWCC 2 cut(s) 188, 492
VspI ATTAAT 1 cut(s) 1340
XapI RAATTY 3 cut(s) 1008, 1520, 1912
XceI RCATGY 3 cut(s) 879, 1534, 1678
XhoI CTCGAG 1 cut(s) 1387
XmaJI CCTAGG 1 cut(s) 380
XmiI GTMKAC 1 cut(s) 15
XmnI GAANNNNTTC 1 cut(s) 918
XspI CTAG 4 cut(s) 381, 543, 933, 1122
ZrmI AGTACT 3 cut(s) 1160, 1169, 1814
Zsp2I ATGCAT 1 cut(s) 1930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.