MD14G1222700.v1.1

Partial alpha/beta-hydrolase lipase region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011380
Physical Location & Seq
Reverse (-)
30418163 .. 30422427
4265 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1222700.v1.1.491

Sequence Viewer

Length: 2106 bp
ATGATTCAACTGTTCGTTGACAACATCATCGCCGTCACCAAAGAGTCAGTGAAGACGTTAACGTACGAATCGCTGAACAATATCGTGCGGCTGATCAATGGAATTTCGGCTCTGTTGCTGACGTTTCTGCCGGGGAAGGCGAACATTCTCGAAGGCATTCATGGGTGGGAGCTCAGGCCCACCTTTCGCGGGCCCAGATTTCCTCGCTGGATGGAGAATGGGGTGTCCTCTTTCAATCGTTTTGTACACGAGCTTTCTGTGGATTCTGATTCTGATGGTTCGAACCTTGATTACGCATACGGAAATGAAGATAGTGATGATGACATATCTCCTACATCACCATCCTCTCAAAGTTCAAGAGCCTCTCGGGCTTCTAGTTTCAGTAAGCACGGTAGGCGCTGGTCGTGGTGGATGTGGATCAGATACATATTTTTCTGGATTCTGTTTCCCGCGAGGTTTCTGTTTGGGATTCCAGTTCGAATTTTGCAATTGTCTTATTCTAGGTCATCAAAAGCCTCTTCTGCACCAGGAAGCAATGAGGCTCCACAAACACGCCCTATAAATAAAGTGCAGATTCTCAAGGATCAAATTGTTCACCGAACCACTGATAGGAGACGTGGAGTCATTGAGGATCTTCATCTAGCAATCGAGATATTCATAGAAGCTGTTTTTGATGTTGTTCACAAGGCAGCGCATTTTGTTCTTTCCCCTTCTGTGGCTTTTAAGACACTATCAGGATGGTGTTCACTGAGCAGCGGTGGTGTTCAAGATAGCCACAGTAGTGATACAGAAGTTACTGTTCCAACTGCTACACTTGGAGAGAATGATCCAGCTCCTAAGGAAAGGAATACTTCATTTCAGCAGTCCCTAAATACCGATGCTCGGACTTGTCAGGATGTCATAACAGAGCTTGGGTACCCATATGAAGCTATTCATGTGATCACTAGTGATGGCTATGTTCTTCTCTTGGAAAGACTCCCGAGACGTGATTCCCGGAAAGCTGTTTATCTACAACATGGAATTTTGGATTCATCTATGGGTTGGGTGTCTAATGGGGTTGTTGGTTCACCGGCATTTGCTGCCTATGATCAAGGATATGATGTTTTTCTCGGTAACTTTCGTGGCTTGATTTCTAGGGAACATGTTAACAAAAATATCTCATCACGACAGTATTGGCAGTACTCCATCAATGAGCATGGGACTCAGGATATTCCTGCAATGATAGAGAAGATTCACCAAGTTAAAACTGCTGAATTGAAACTTAGTCAACCTGATGTTGACGAAGAAACAAATGATGGTCAACGATACAAGCTCTGTGCAATTTGCCATAGCTTGGGAGGAGCAGCAATACTTATGTACATTATAACGCAGAGAATAGAAGAGAAACCCCACAGACTCTCTAGGCTGGTTTTACTGTCACCTGCTGGCTTCCATGGTGATTCTACCTTTATGTTTACGGTTGTGGAGAATGTCTTTCTTCTATTGGCTCCTCTTCTGGCTCCTCTTGTGCCTGCCTTTTACATACCAACCAGGTTCTTTCGCATGCTTCTGAACAAGTTGGCGCGAGACTTTCATAACTATCCTGCTGTCGGAGGACTGGTTCAGACCCTGATGAGTTATTTTGTTGGTGGGGATAGCTCAAATTGGGTTGGTGTGTTAGGGATACCACATTATAACATGAACGACATGCCGGGCGTATCATTCCGAGTGGCTCTCCATCTTGCACAAATCAAGCGTGCTGGAAAGTTTAGAATGTTCGATTACGGGAATGGTTCTGCTAATATGGAGGTGTATGGATCTCCAGAGCCATTCGACTTGGGGGAGTACTATGGGCTTATTGATGTTCCAGTTGATCTGGTTGCTGGACGAAAGGACAAGGTAATTCGGCCATCAATGGTCAGAAAACATTATAAGTTGATGGACGAATCAGGTGTCGATGTATCATATGGTGAATTTGAGTACGCCCATCTGGACTTCACATTTTCGCACCGAGAAGAACTATTGGCGTATGCCATGTCACGCTTGCTGCTTGTTGAGCCAACTCCGCAGCAGCAATCCAGTCAGAAGGCCGTAAGGTTGAAGAAGAAAACCCCGGCCAATAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydro_lipase

Protein Analysis

702

Amino Acids

78.91

Weight (kDa)

6.9

Isoelectric Point (pI)

53.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydro_lipase PF04083 298 - 352 4.2e-14 Partial alpha/beta-hydrolase lipase region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011380)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18460 AT1G73920 AT1G73920
fragaria_vesca FvH4_5g12600 FvH4_5g12600
malus_domestica MD06G1212200.v1.1 MD14G1222700.v1.1
prunus_persica Prupe.5G218200_v2.0.a1
pyrus_communis pycom06g18940 pycom14g18440
rosa_chinensis RchiOBHm_Chr7g0184731
rosa_laevigata RLG00000004955
rosa_multiflora Rmu_ssc0000289.1_g000019
rosa_roxburghii Rroxscaffold_3G00269390
rosa_rugosa Rorug06G0464800
rosa_samantha Rh7AG078100 Rh7BG069200 Rh7CG070000 Rh7DG069400
rosa_wichuraiana Rw7G005730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1364, 1674, 1911
AarI CACCTGC 1 cut(s) 1429
Acc36I ACCTGC 1 cut(s) 1429
Acc65I GGTACC 1 cut(s) 915
AccB1I GGYRCC 1 cut(s) 915
AccB7I CCANNNNNTGG 1 cut(s) 1333
AccII CGCG 3 cut(s) 189, 452, 1564
AciI CCGC 5 cut(s) 88, 189, 450, 756, 2045
AclWI GGATC 5 cut(s) 425, 591, 639, 821, 1804
AcoI YGGCCR 2 cut(s) 1886, 2094
AcsI RAATTY 4 cut(s) 102, 480, 1020, 1952
AfaI GTAC 7 cut(s) 65, 246, 917, 1181, 1358, 1826, 1961
AfiI CCNNNNNNNGG 6 cut(s) 189, 609, 715, 882, 1333, 1589
AflIII ACRYGT 1 cut(s) 1141
AgsI TTSAA 6 cut(s) 8, 235, 357, 767, 1258, 2080
AhdI GACNNNNNGTC 1 cut(s) 620
AhlI ACTAGT 1 cut(s) 944
AjiI CACGTC 2 cut(s) 617, 986
AjnI CCWGG 2 cut(s) 526, 1529
AleI CACNNNNGTG 1 cut(s) 780
AloI GAACNNNNNNTCC 2 cut(s) 1584, 1616
Alw21I GWGCWC 1 cut(s) 174
Alw26I GTCTC 3 cut(s) 607, 976, 1560
AlwI GGATC 5 cut(s) 425, 591, 639, 821, 1804
AlwNI CAGNNNCTG 1 cut(s) 1609
Ama87I CYCGRG 2 cut(s) 366, 979
AoxI GGCC 5 cut(s) 176, 191, 1886, 2067, 2094
ApaI GGGCCC 1 cut(s) 195
ApeKI GCWGC 7 cut(s) 689, 753, 1079, 1343, 2026, 2047, 2050
ApoI RAATTY 4 cut(s) 102, 480, 1020, 1952
ArsI GACNNNNNNTTYG 2 cut(s) 1917, 1949
Asp700I GAANNNNTTC 2 cut(s) 156, 930
Asp718I GGTACC 1 cut(s) 915
AspLEI GCGC 3 cut(s) 399, 694, 1564
AspS9I GGNCC 3 cut(s) 177, 191, 192
AsuC2I CCSGG 4 cut(s) 132, 994, 1692, 2093
AsuHPI GGTGA 8 cut(s) 28, 330, 587, 1059, 1226, 1410, 1448, 1961
AsuII TTCGAA 2 cut(s) 281, 478
AvaI CYCGRG 2 cut(s) 366, 979
AxyI CCTNAGG 1 cut(s) 837
BaeGI GKGCMC 1 cut(s) 195
BaeI ACNNNNGTAYC 2 cut(s) 777, 810
BanI GGYRCC 1 cut(s) 915
BanII GRGCYC 2 cut(s) 174, 195
BauI CACGAG 1 cut(s) 248
BbsI GAAGAC 1 cut(s) 59
Bbv12I GWGCWC 1 cut(s) 174
BbvI GCAGC 7 cut(s) 701, 765, 1066, 1355, 2013, 2059, 2062
BceAI ACGGC 2 cut(s) 17, 2054
BciT130I CCWGG 2 cut(s) 528, 1531
BciVI GTATCC 1 cut(s) 1656
BclI TGATCA 3 cut(s) 93, 939, 1087
BcnI CCSGG 4 cut(s) 132, 994, 1692, 2093
BcoDI GTCTC 3 cut(s) 607, 976, 1560
BcuI ACTAGT 1 cut(s) 944
BfaI CTAG 6 cut(s) 375, 501, 641, 945, 1134, 1401
BfoI RGCGCY 1 cut(s) 400
BfuAI ACCTGC 1 cut(s) 1429
BfuI GTATCC 1 cut(s) 1656
BglI GCCNNNNNGGC 1 cut(s) 368
BisI GCNGC 8 cut(s) 89, 690, 754, 1080, 1344, 2027, 2048, 2051
BlsI GCNGC 8 cut(s) 90, 691, 755, 1081, 1345, 2028, 2049, 2052
BmcAI AGTACT 2 cut(s) 1181, 1826
Bme1390I CCNGG 6 cut(s) 132, 528, 994, 1531, 1692, 2093
BmeRI GACNNNNNGTC 1 cut(s) 620
BmeT110I CYCGRG 2 cut(s) 366, 979
BmgBI CACGTC 2 cut(s) 617, 986
BmgT120I GGNCC 3 cut(s) 177, 191, 192
BmiI GGNNCC 5 cut(s) 193, 543, 917, 1488, 1500
BmrFI CCNGG 6 cut(s) 132, 528, 994, 1531, 1692, 2093
BmsI GCATC 1 cut(s) 868
BpiI GAAGAC 1 cut(s) 59
BplI GAGNNNNNCTC 2 cut(s) 1698, 1730
BpmI CTGGAG 1 cut(s) 1785
Bpu10I CCTNAGC 1 cut(s) 173
Bpu14I TTCGAA 2 cut(s) 281, 478
BpuEI CTTGAG 1 cut(s) 563
BpuMI CCSGG 4 cut(s) 132, 994, 1692, 2093
BsaBI GATNNNNATC 2 cut(s) 416, 636
BsaJI CCNNGG 3 cut(s) 131, 1432, 2091
BsaXI ACNNNNNCTCC 2 cut(s) 1584, 1614
Bsc4I CCNNNNNNNGG 6 cut(s) 189, 609, 715, 882, 1333, 1589
Bse118I RCCGGY 1 cut(s) 1069
Bse1I ACTGG 4 cut(s) 473, 1602, 1847, 2058
Bse21I CCTNAGG 1 cut(s) 837
Bse3DI GCAATG 2 cut(s) 541, 1224
Bse8I GATNNNNATC 2 cut(s) 416, 636
BseBI CCWGG 2 cut(s) 528, 1531
BseDI CCNNGG 3 cut(s) 131, 1432, 2091
BseGI GGATG 5 cut(s) 216, 341, 417, 743, 901
BseJI GATNNNNATC 2 cut(s) 416, 636
BseLI CCNNNNNNNGG 6 cut(s) 189, 609, 715, 882, 1333, 1589
BseMI GCAATG 2 cut(s) 541, 1224
BseMII CTCAG 3 cut(s) 187, 740, 1217
BseNI ACTGG 4 cut(s) 473, 1602, 1847, 2058
BseRI GAGGAG 3 cut(s) 1353, 1479, 1491
BseSI GKGCMC 1 cut(s) 195
BseXI GCAGC 7 cut(s) 701, 765, 1066, 1355, 2013, 2059, 2062
BsgI GTGCAG 2 cut(s) 507, 590
Bsh1236I CGCG 3 cut(s) 189, 452, 1564
BshFI GGCC 5 cut(s) 178, 193, 1888, 2069, 2096
BshNI GGYRCC 1 cut(s) 915
BsiHKAI GWGCWC 1 cut(s) 174
BsiHKCI CYCGRG 2 cut(s) 366, 979
BsiSI CCGG 5 cut(s) 131, 994, 1070, 1691, 2093
BsiWI CGTACG 1 cut(s) 63
BslFI GGGAC 2 cut(s) 850, 1213
BslI CCNNNNNNNGG 6 cut(s) 189, 609, 715, 882, 1333, 1589
BsmAI GTCTC 3 cut(s) 607, 976, 1560
BsmBI CGTCTC 2 cut(s) 607, 976
BsmFI GGGAC 2 cut(s) 850, 1213
BsmI GAATGC 1 cut(s) 156
BsnI GGCC 5 cut(s) 178, 193, 1888, 2069, 2096
BsoBI CYCGRG 2 cut(s) 366, 979
Bsp119I TTCGAA 2 cut(s) 281, 478
Bsp120I GGGCCC 1 cut(s) 191
Bsp1286I GDGCHC 2 cut(s) 174, 195
Bsp1407I TGTACA 2 cut(s) 244, 1356
Bsp143I GATC 9 cut(s) 93, 417, 583, 631, 826, 939, 1087, 1796, 1852
Bsp19I CCATGG 1 cut(s) 1432
BspACI CCGC 5 cut(s) 88, 189, 450, 756, 2045
BspANI GGCC 5 cut(s) 178, 193, 1888, 2069, 2096
BspCNI CTCAG 3 cut(s) 186, 741, 1216
BspFNI CGCG 3 cut(s) 189, 452, 1564
BspLI GGNNCC 5 cut(s) 193, 543, 917, 1488, 1500
BspMI ACCTGC 1 cut(s) 1429
BspPI GGATC 5 cut(s) 425, 591, 639, 821, 1804
BspT104I TTCGAA 2 cut(s) 281, 478
BspT107I GGYRCC 1 cut(s) 915
BsrDI GCAATG 2 cut(s) 541, 1224
BsrFI RCCGGY 1 cut(s) 1069
BsrGI TGTACA 2 cut(s) 244, 1356
BsrI ACTGG 4 cut(s) 473, 1602, 1847, 2058
BssAI RCCGGY 1 cut(s) 1069
BssECI CCNNGG 3 cut(s) 131, 1432, 2091
BssMI GATC 9 cut(s) 93, 417, 583, 631, 826, 939, 1087, 1796, 1852
BssSI CACGAG 1 cut(s) 248
BssT1I CCWWGG 1 cut(s) 1432
Bst2BI CACGAG 1 cut(s) 248
Bst2UI CCWGG 2 cut(s) 528, 1531
Bst4CI ACNGT 7 cut(s) 12, 392, 779, 799, 1170, 1416, 1459
Bst6I CTCTTC 3 cut(s) 523, 1374, 1497
BstAPI GCANNNNNTGC 1 cut(s) 1079
BstAUI TGTACA 2 cut(s) 244, 1356
BstBI TTCGAA 2 cut(s) 281, 478
BstC8I GCNNGC 6 cut(s) 191, 1426, 1512, 1544, 1737, 2024
BstDEI CTNAG 5 cut(s) 173, 749, 837, 1203, 1262
BstDSI CCRYGG 1 cut(s) 1432
BstF5I GGATG 5 cut(s) 216, 341, 417, 743, 901
BstFNI CGCG 3 cut(s) 189, 452, 1564
BstH2I RGCGCY 1 cut(s) 400
BstHHI GCGC 3 cut(s) 399, 694, 1564
BstKTI GATC 9 cut(s) 96, 420, 586, 634, 829, 942, 1090, 1799, 1855
BstMAI GTCTC 3 cut(s) 607, 976, 1560
BstMBI GATC 9 cut(s) 93, 417, 583, 631, 826, 939, 1087, 1796, 1852
BstMWI GCNNNNNNNGC 6 cut(s) 368, 394, 521, 1079, 2035, 2044
BstNI CCWGG 2 cut(s) 528, 1531
BstNSI RCATGY 3 cut(s) 1145, 1546, 1690
BstSCI CCNGG 6 cut(s) 130, 526, 992, 1529, 1690, 2091
BstSLI GKGCMC 1 cut(s) 195
BstUI CGCG 3 cut(s) 189, 452, 1564
BstV1I GCAGC 7 cut(s) 701, 765, 1066, 1355, 2013, 2059, 2062
BstV2I GAAGAC 1 cut(s) 59
BstX2I RGATCY 2 cut(s) 631, 1796
BstYI RGATCY 2 cut(s) 631, 1796
Bsu36I CCTNAGG 1 cut(s) 837
BsuI GTATCC 1 cut(s) 1656
BsuRI GGCC 5 cut(s) 178, 193, 1888, 2069, 2096
BtgI CCRYGG 1 cut(s) 1432
BtgZI GCGATG 1 cut(s) 13
BtrI CACGTC 2 cut(s) 617, 986
BtsCI GGATG 5 cut(s) 216, 341, 417, 743, 901
BtsIMutI CAGTG 3 cut(s) 54, 603, 746
BveI ACCTGC 1 cut(s) 1429
Cac8I GCNNGC 6 cut(s) 191, 1426, 1512, 1544, 1737, 2024
CaiI CAGNNNCTG 1 cut(s) 1609
CfoI GCGC 3 cut(s) 399, 694, 1564
Cfr10I RCCGGY 1 cut(s) 1069
Cfr13I GGNCC 3 cut(s) 177, 191, 192
CsiI ACCWGGT 1 cut(s) 1529
Csp6I GTAC 7 cut(s) 64, 245, 916, 1180, 1357, 1825, 1960
CviQI GTAC 7 cut(s) 64, 245, 916, 1180, 1357, 1825, 1960
DdeI CTNAG 5 cut(s) 173, 749, 837, 1203, 1262
DpnI GATC 9 cut(s) 95, 419, 585, 633, 828, 941, 1089, 1798, 1854
DpnII GATC 9 cut(s) 93, 417, 583, 631, 826, 939, 1087, 1796, 1852
DriI GACNNNNNGTC 1 cut(s) 620
EaeI YGGCCR 2 cut(s) 1886, 2094
Eam1104I CTCTTC 3 cut(s) 523, 1374, 1497
Eam1105I GACNNNNNGTC 1 cut(s) 620
EarI CTCTTC 3 cut(s) 523, 1374, 1497
Ecl136II GAGCTC 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 1432
Eco24I GRGCYC 2 cut(s) 174, 195
Eco53kI GAGCTC 1 cut(s) 172
Eco81I CCTNAGG 1 cut(s) 837
Eco88I CYCGRG 2 cut(s) 366, 979
EcoICRI GAGCTC 1 cut(s) 172
EcoRII CCWGG 2 cut(s) 526, 1529
EcoT14I CCWWGG 1 cut(s) 1432
EcoT38I GRGCYC 2 cut(s) 174, 195
ErhI CCWWGG 1 cut(s) 1432
Esp3I CGTCTC 2 cut(s) 607, 976
FalI AAGNNNNNCTT 2 cut(s) 835, 867
FaqI GGGAC 2 cut(s) 850, 1213
FauI CCCGC 2 cut(s) 182, 457
FauNDI CATATG 2 cut(s) 922, 1945
FbaI TGATCA 3 cut(s) 93, 939, 1087
Fnu4HI GCNGC 8 cut(s) 89, 690, 754, 1080, 1344, 2027, 2048, 2051
FokI GGATG 5 cut(s) 223, 328, 424, 750, 908
FriOI GRGCYC 2 cut(s) 174, 195
Fsp4HI GCNGC 8 cut(s) 89, 690, 754, 1080, 1344, 2027, 2048, 2051
FspBI CTAG 6 cut(s) 375, 501, 641, 945, 1134, 1401
GlaI GCGC 3 cut(s) 398, 693, 1563
GluI GCNGC 8 cut(s) 89, 690, 754, 1080, 1344, 2027, 2048, 2051
GsuI CTGGAG 1 cut(s) 1785
HaeII RGCGCY 1 cut(s) 400
HaeIII GGCC 5 cut(s) 178, 193, 1888, 2069, 2096
HapII CCGG 5 cut(s) 131, 994, 1070, 1691, 2093
HhaI GCGC 3 cut(s) 399, 694, 1564
Hin6I GCGC 3 cut(s) 397, 692, 1562
HinP1I GCGC 3 cut(s) 397, 692, 1562
HincII GTYRAC 6 cut(s) 19, 60, 1147, 1268, 1279, 1301
HindII GTYRAC 6 cut(s) 19, 60, 1147, 1268, 1279, 1301
HpaI GTTAAC 2 cut(s) 60, 1147
HpaII CCGG 5 cut(s) 131, 994, 1070, 1691, 2093
HphI GGTGA 8 cut(s) 28, 330, 587, 1059, 1226, 1410, 1448, 1961
HpyAV CCTTC 4 cut(s) 130, 146, 720, 2059
HpyCH4III ACNGT 7 cut(s) 12, 392, 779, 799, 1170, 1416, 1459
HpyCH4IV ACGT 5 cut(s) 56, 62, 122, 616, 985
HpyCH4V TGCA 6 cut(s) 487, 524, 571, 1217, 1319, 1724
HpyF10VI GCNNNNNNNGC 6 cut(s) 368, 394, 521, 1079, 2035, 2044
HpyF3I CTNAG 5 cut(s) 173, 749, 837, 1203, 1262
HpySE526I ACGT 5 cut(s) 56, 62, 122, 616, 985
HspAI GCGC 3 cut(s) 397, 692, 1562
KpnI GGTACC 1 cut(s) 919
Ksp22I TGATCA 3 cut(s) 93, 939, 1087
KspAI GTTAAC 2 cut(s) 60, 1147
Kzo9I GATC 9 cut(s) 93, 417, 583, 631, 826, 939, 1087, 1796, 1852
LmnI GCTCC 6 cut(s) 169, 547, 838, 1340, 1492, 1504
Lsp1109I GCAGC 7 cut(s) 701, 765, 1066, 1355, 2013, 2059, 2062
LweI GCATC 1 cut(s) 868
MabI ACCWGGT 1 cut(s) 1529
MaeI CTAG 6 cut(s) 375, 501, 641, 945, 1134, 1401
MaeII ACGT 5 cut(s) 56, 62, 122, 616, 985
MaeIII GTNAC 5 cut(s) 34, 793, 1112, 1416, 2016
MalI GATC 9 cut(s) 95, 419, 585, 633, 828, 941, 1089, 1798, 1854
MboI GATC 9 cut(s) 93, 417, 583, 631, 826, 939, 1087, 1796, 1852
MfeI CAATTG 1 cut(s) 488
MflI RGATCY 2 cut(s) 631, 1796
MhlI GDGCHC 2 cut(s) 174, 195
MlyI GAGTC 5 cut(s) 53, 630, 969, 1195, 1389
MmeI TCCRAC 2 cut(s) 827, 1570
MroXI GAANNNNTTC 2 cut(s) 156, 930
MseI TTAA 4 cut(s) 59, 723, 1146, 1242
MslI CAYNNNNRTG 1 cut(s) 780
MspA1I CMGCKG 1 cut(s) 756
MspI CCGG 5 cut(s) 131, 994, 1070, 1691, 2093
MspR9I CCNGG 6 cut(s) 132, 528, 994, 1531, 1692, 2093
MunI CAATTG 1 cut(s) 488
Mva1269I GAATGC 1 cut(s) 156
MvaI CCWGG 2 cut(s) 528, 1531
MvnI CGCG 3 cut(s) 189, 452, 1564
MwoI GCNNNNNNNGC 6 cut(s) 368, 394, 521, 1079, 2035, 2044
NciI CCSGG 4 cut(s) 132, 994, 1692, 2093
NcoI CCATGG 1 cut(s) 1432
NdeI CATATG 2 cut(s) 922, 1945
NdeII GATC 9 cut(s) 93, 417, 583, 631, 826, 939, 1087, 1796, 1852
NlaIV GGNNCC 5 cut(s) 193, 543, 917, 1488, 1500
NmuCI GTSAC 3 cut(s) 34, 1416, 2016
NspI RCATGY 3 cut(s) 1145, 1546, 1690
NspV TTCGAA 2 cut(s) 281, 478
OliI CACNNNNGTG 1 cut(s) 780
PaeI GCATGC 1 cut(s) 1546
PaqCI CACCTGC 1 cut(s) 1429
PciI ACATGT 1 cut(s) 1141
PctI GAATGC 1 cut(s) 156
PdmI GAANNNNTTC 2 cut(s) 156, 930
Pfl23II CGTACG 1 cut(s) 63
PflMI CCANNNNNTGG 1 cut(s) 1333
PfoI TCCNGGA 1 cut(s) 992
PkrI GCNGC 8 cut(s) 90, 691, 755, 1081, 1345, 2028, 2049, 2052
PleI GAGTC 5 cut(s) 52, 629, 969, 1195, 1389
PpsI GAGTC 5 cut(s) 52, 629, 969, 1195, 1389
PscI ACATGT 1 cut(s) 1141
PsiI TTATAA 3 cut(s) 1364, 1674, 1911
Psp124BI GAGCTC 1 cut(s) 174
Psp6I CCWGG 2 cut(s) 526, 1529
PspGI CCWGG 2 cut(s) 526, 1529
PspLI CGTACG 1 cut(s) 63
PspN4I GGNNCC 5 cut(s) 193, 543, 917, 1488, 1500
PspOMI GGGCCC 1 cut(s) 191
PspPI GGNCC 3 cut(s) 177, 191, 192
PsrI GAACNNNNNNTAC 2 cut(s) 275, 307
PstNI CAGNNNCTG 1 cut(s) 1609
PsuI RGATCY 2 cut(s) 631, 1796
RsaI GTAC 7 cut(s) 65, 246, 917, 1181, 1358, 1826, 1961
RsaNI GTAC 7 cut(s) 64, 245, 916, 1180, 1357, 1825, 1960
RseI CAYNNNNRTG 1 cut(s) 780
SacI GAGCTC 1 cut(s) 174
SaqAI TTAA 4 cut(s) 59, 723, 1146, 1242
SatI GCNGC 8 cut(s) 89, 690, 754, 1080, 1344, 2027, 2048, 2051
Sau3AI GATC 9 cut(s) 93, 417, 583, 631, 826, 939, 1087, 1796, 1852
Sau96I GGNCC 3 cut(s) 177, 191, 192
ScaI AGTACT 2 cut(s) 1181, 1826
SchI GAGTC 5 cut(s) 53, 630, 969, 1195, 1389
ScrFI CCNGG 6 cut(s) 132, 528, 994, 1531, 1692, 2093
SduI GDGCHC 2 cut(s) 174, 195
SexAI ACCWGGT 1 cut(s) 1529
SfaNI GCATC 1 cut(s) 868
SfuI TTCGAA 2 cut(s) 281, 478
SmiMI CAYNNNNRTG 1 cut(s) 780
SmlI CTYRAG 1 cut(s) 578
SmoI CTYRAG 1 cut(s) 578
SpeI ACTAGT 1 cut(s) 944
SphI GCATGC 1 cut(s) 1546
SsiI CCGC 5 cut(s) 88, 189, 450, 756, 2045
SspMI CTAG 6 cut(s) 375, 501, 641, 945, 1134, 1401
SstI GAGCTC 1 cut(s) 174
StyD4I CCNGG 6 cut(s) 130, 526, 992, 1529, 1690, 2091
StyI CCWWGG 1 cut(s) 1432
TaaI ACNGT 7 cut(s) 12, 392, 779, 799, 1170, 1416, 1459
TaiI ACGT 5 cut(s) 59, 65, 125, 619, 988
TaqI TCGA 7 cut(s) 150, 281, 478, 648, 1758, 1812, 1935
TatI WGTACW 4 cut(s) 244, 1179, 1356, 1824
TauI GCSGC 1 cut(s) 91
Tru1I TTAA 4 cut(s) 59, 723, 1146, 1242
Tru9I TTAA 4 cut(s) 59, 723, 1146, 1242
TscAI CASTG 3 cut(s) 54, 610, 753
TseFI GTSAC 3 cut(s) 34, 1416, 2016
TseI GCWGC 7 cut(s) 689, 753, 1079, 1343, 2026, 2047, 2050
Tsp45I GTSAC 3 cut(s) 34, 1416, 2016
TspGWI ACGGA 1 cut(s) 315
TspRI CASTG 3 cut(s) 54, 610, 753
Van91I CCANNNNNTGG 1 cut(s) 1333
XapI RAATTY 4 cut(s) 102, 480, 1020, 1952
XceI RCATGY 3 cut(s) 1145, 1546, 1690
XmnI GAANNNNTTC 2 cut(s) 156, 930
XspI CTAG 6 cut(s) 375, 501, 641, 945, 1134, 1401
ZrmI AGTACT 2 cut(s) 1181, 1826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.