Rorug06G0464800

Partial alpha/beta-hydrolase lipase region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0011380
Physical Location & Seq
Reverse (-)
58942840 .. 58944777
1938 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0464800.1

Sequence Viewer

Length: 1557 bp
ATGGGAAATCATTTGTTGAATGGCAATGTGCCAATATACTTACACTTTGACATAAGCGTAAGCAGCTACAAAAACCAAACGAAAGTTTGGAACTTCAATCCCTGCAGCTATGCTTTTCTTGTCCAAGAGGGTAAGTTCAACTTCACCTCCGACATGTTGAGTGATTTAAGAAATATCTCTCAGTTTCCCGTGGTCCTCGATTGGTCAATTGGAAATGAGACATGCAGCCAGATCAAGAACAATGCATGTAAGGGGAACAGCAACTGTGTGGCTGTCGAGAATGGGCTCGGGTATCGCTGCAAGTGCAACGAAGGTTACGAAGGGAATCCATACCTAAATGGCTGCACTGGACTCGGTGTTGGCCTCATATCCTTATTAATAGGCAGCTCCTGGTTGTACTTGGTCTACAAAAGATGGAAGCTCATGAAGGTGAAGGAGAAGTTCTTCATCCAAAATGGAGGATTGTTGTTAAAGCAACAGCTTTCGAAACGACAAGGTGGTGCCACTAACCAAACGGCCAAAATCTTCACGGAAGACCAACTTAAGAAGGCTACCAACCATTTCAGTGAAGCCAGAATCGTTGGCAAAGGAGGTTTCGGTACAGTCTACAAAGGGATTATAGCAGATGAAAGAGCTAAGGAAACAGTGGTTGCAATCAAGAAATCCAAACTTGTGGATCGAAGCCAAATAGAGCAATTCATTAATGAAGTACAGGCATGTGGACATGTGGTGAAGCTTTTGGGTTGTTGTTTCGAGACAGAAGTCCCACTACTAGTTTATGAGTTTGTCACCAATGGTACGCTATTCGACTACATCCACAACAAAAGCAAGGCATCCAACTTTGCTTGGGAAATTCGTTTAAGGATAGCTGCAGAAACTGCTGGAGTGCTATCACACTTGCACTCTGAAGCTTCTATTCCCATCATTCACAGAGATGTCAAGTCCACAAACATACTCTTGGATGATAATCTTACAGCAAAAGTGTCCGATTTCGGTGCCTCCAGATTGGTTCCGTCAGATCAAGCTCAGCTATCTACAGTGGTGCAAGGAACGGTAGGGTATTTGGACCCTGAATACTTACAGACGAGCCAGTTGACTGACAAGAGTGATGTCTATAGCTTTGGGGTTGTTCTTGTGGAACTACTAACAGGAAAGAAGGCGCTTTCGTTCGACAAGCCGGAAAAGGAGAGAAATCTAGCCATGTATTTTCTTTCTGCCCTGAGAGAGGACCGATTGGTAGAGGTGATTGATGGTAGTGCCATTAACCGAGCAAACGTTGAGCAGCTTAAGGAAGTTTCAAACCTTGCAAGGAGGTGTTTGAAAGTAAAAGGAAAAGAAAGACCAACCATGAAGGAAGTGGCCATGGAGCTCGAGGGACTGAGAAGGATGGTAATGCATCCATGGGTTAATAATGATGAATCCAATGCAGAGGAGAATGAGCACTTGCTTGGTGAAATAAGACTGGAAACTTTTAGTCATGGAGGTGGTGGGGATACGAGTTCTGGGTATGATACCATGAGGAACCAGATCACATTACCAGTTGATGATGGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydro_lipase

Protein Analysis

518

Amino Acids

57.78

Weight (kDa)

7.14

Isoelectric Point (pI)

26.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 189 - 453 1.7e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 192 - 457 6.3e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011380)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18460 AT1G73920 AT1G73920
fragaria_vesca FvH4_5g12600 FvH4_5g12600
malus_domestica MD06G1212200.v1.1 MD14G1222700.v1.1
prunus_persica Prupe.5G218200_v2.0.a1
pyrus_communis pycom06g18940 pycom14g18440
rosa_chinensis RchiOBHm_Chr7g0184731
rosa_laevigata RLG00000004955
rosa_multiflora Rmu_ssc0000289.1_g000019
rosa_roxburghii Rroxscaffold_3G00269390
rosa_rugosa Rorug06G0464800
rosa_samantha Rh7AG078100 Rh7BG069200 Rh7CG070000 Rh7DG069400
rosa_wichuraiana Rw7G005730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 500, 995
AccI GTMKAC 2 cut(s) 405, 606
AclI AACGTT 1 cut(s) 1275
AclWI GGATC 1 cut(s) 684
AcoI YGGCCR 2 cut(s) 516, 1359
AcsI RAATTY 1 cut(s) 852
AcuI CTGAAG 1 cut(s) 927
AfaI GTAC 4 cut(s) 398, 601, 711, 799
AfiI CCNNNNNNNGG 1 cut(s) 1225
AflII CTTAAG 2 cut(s) 542, 1286
AflIII ACRYGT 2 cut(s) 153, 724
AgsI TTSAA 5 cut(s) 19, 97, 139, 1299, 1321
AhlI ACTAGT 1 cut(s) 772
AjnI CCWGG 1 cut(s) 389
Alw21I GWGCWC 2 cut(s) 1371, 1443
Alw26I GTCTC 2 cut(s) 212, 749
AlwI GGATC 1 cut(s) 684
AlwNI CAGNNNCTG 3 cut(s) 264, 390, 878
Ama87I CYCGRG 2 cut(s) 287, 1370
AoxI GGCC 3 cut(s) 361, 516, 1359
ApeKI GCWGC 8 cut(s) 63, 105, 225, 297, 342, 384, 869, 1282
ApoI RAATTY 1 cut(s) 852
AseI ATTAAT 2 cut(s) 377, 702
Asp700I GAANNNNTTC 1 cut(s) 443
AspLEI GCGC 1 cut(s) 1162
AspS9I GGNCC 3 cut(s) 193, 1066, 1228
AsuHPI GGTGA 6 cut(s) 136, 442, 742, 781, 1255, 1463
AsuII TTCGAA 1 cut(s) 485
AvaI CYCGRG 2 cut(s) 287, 1370
AvaII GGWCC 3 cut(s) 193, 1066, 1228
BalI TGGCCA 1 cut(s) 1361
BanI GGYRCC 2 cut(s) 500, 995
BanII GRGCYC 2 cut(s) 288, 1371
BarI GAAGNNNNNNTAC 2 cut(s) 753, 785
BbsI GAAGAC 1 cut(s) 540
Bbv12I GWGCWC 2 cut(s) 1371, 1443
BbvI GCAGC 8 cut(s) 75, 117, 237, 284, 329, 396, 856, 1294
BccI CCATC 5 cut(s) 408, 929, 1244, 1381, 1541
BceAI ACGGC 1 cut(s) 531
BcgI CGANNNNNNTGC 4 cut(s) 334, 368, 977, 1011
BciT130I CCWGG 1 cut(s) 391
BciVI GTATCC 1 cut(s) 1486
BcoDI GTCTC 2 cut(s) 212, 749
BcuI ACTAGT 1 cut(s) 772
BfaI CTAG 2 cut(s) 773, 1196
BfmI CTRYAG 4 cut(s) 103, 870, 1035, 1114
BfoI RGCGCY 1 cut(s) 1163
BfrI CTTAAG 2 cut(s) 542, 1286
BfuI GTATCC 1 cut(s) 1486
BisI GCNGC 8 cut(s) 64, 106, 226, 298, 343, 385, 870, 1283
BlpI GCTNAGC 1 cut(s) 1026
BlsI GCNGC 8 cut(s) 65, 107, 227, 299, 344, 386, 871, 1284
Bme1390I CCNGG 1 cut(s) 391
Bme18I GGWCC 3 cut(s) 193, 1066, 1228
BmeT110I CYCGRG 2 cut(s) 287, 1370
BmgT120I GGNCC 3 cut(s) 193, 1066, 1228
BmiI GGNNCC 5 cut(s) 502, 997, 1011, 1068, 1523
BmrFI CCNGG 1 cut(s) 391
BmsI GCATC 2 cut(s) 842, 1405
BoxI GACNNNNGTC 1 cut(s) 761
BpiI GAAGAC 1 cut(s) 540
BpmI CTGGAG 2 cut(s) 903, 985
Bpu10I CCTNAGC 1 cut(s) 636
Bpu1102I GCTNAGC 1 cut(s) 1026
Bpu14I TTCGAA 1 cut(s) 485
BsaBI GATNNNNATC 1 cut(s) 966
BsaJI CCNNGG 3 cut(s) 189, 1362, 1400
BsaXI ACNNNNNCTCC 2 cut(s) 131, 161
Bsc4I CCNNNNNNNGG 1 cut(s) 1225
Bse1I ACTGG 4 cut(s) 352, 1090, 1467, 1538
Bse3DI GCAATG 1 cut(s) 31
Bse8I GATNNNNATC 1 cut(s) 966
BseBI CCWGG 1 cut(s) 391
BseDI CCNNGG 3 cut(s) 189, 1362, 1400
BseGI GGATG 6 cut(s) 447, 813, 833, 967, 1392, 1396
BseJI GATNNNNATC 1 cut(s) 966
BseLI CCNNNNNNNGG 1 cut(s) 1225
BseMI GCAATG 1 cut(s) 31
BseMII CTCAG 4 cut(s) 194, 1040, 1211, 1370
BseNI ACTGG 4 cut(s) 352, 1090, 1467, 1538
BseRI GAGGAG 1 cut(s) 1445
BseXI GCAGC 8 cut(s) 75, 117, 237, 284, 329, 396, 856, 1294
BsgI GTGCAG 1 cut(s) 328
BshFI GGCC 3 cut(s) 363, 518, 1361
BshNI GGYRCC 2 cut(s) 500, 995
BsiHKAI GWGCWC 2 cut(s) 1371, 1443
BsiHKCI CYCGRG 2 cut(s) 287, 1370
BsiSI CCGG 1 cut(s) 1178
BslFI GGGAC 2 cut(s) 749, 1389
BslI CCNNNNNNNGG 1 cut(s) 1225
BsmAI GTCTC 2 cut(s) 212, 749
BsmFI GGGAC 2 cut(s) 749, 1389
BsnI GGCC 3 cut(s) 363, 518, 1361
BsoBI CYCGRG 2 cut(s) 287, 1370
Bsp119I TTCGAA 1 cut(s) 485
Bsp1286I GDGCHC 3 cut(s) 288, 1371, 1443
Bsp143I GATC 4 cut(s) 231, 676, 1018, 1527
Bsp1720I GCTNAGC 1 cut(s) 1026
Bsp19I CCATGG 2 cut(s) 1362, 1400
BspANI GGCC 3 cut(s) 363, 518, 1361
BspCNI CTCAG 4 cut(s) 193, 1039, 1212, 1371
BspHI TCATGA 1 cut(s) 423
BspLI GGNNCC 5 cut(s) 502, 997, 1011, 1068, 1523
BspMAI CTGCAG 2 cut(s) 107, 874
BspPI GGATC 1 cut(s) 684
BspT104I TTCGAA 1 cut(s) 485
BspT107I GGYRCC 2 cut(s) 500, 995
BspTI CTTAAG 2 cut(s) 542, 1286
BsrDI GCAATG 1 cut(s) 31
BsrI ACTGG 4 cut(s) 352, 1090, 1467, 1538
BssECI CCNNGG 3 cut(s) 189, 1362, 1400
BssMI GATC 4 cut(s) 231, 676, 1018, 1527
BssT1I CCWWGG 2 cut(s) 1362, 1400
Bst2UI CCWGG 1 cut(s) 391
Bst4CI ACNGT 5 cut(s) 266, 604, 646, 1039, 1054
BstAFI CTTAAG 2 cut(s) 542, 1286
BstAPI GCANNNNNTGC 1 cut(s) 878
BstBI TTCGAA 1 cut(s) 485
BstDEI CTNAG 5 cut(s) 180, 636, 1026, 1220, 1379
BstDSI CCRYGG 3 cut(s) 189, 1362, 1400
BstENI CCTNNNNNAGG 1 cut(s) 1223
BstF5I GGATG 6 cut(s) 447, 813, 833, 967, 1392, 1396
BstH2I RGCGCY 1 cut(s) 1163
BstHHI GCGC 1 cut(s) 1162
BstKTI GATC 4 cut(s) 234, 679, 1021, 1530
BstMAI GTCTC 2 cut(s) 212, 749
BstMBI GATC 4 cut(s) 231, 676, 1018, 1527
BstMWI GCNNNNNNNGC 3 cut(s) 63, 303, 878
BstNI CCWGG 1 cut(s) 391
BstNSI RCATGY 5 cut(s) 157, 225, 249, 720, 728
BstPAI GACNNNNGTC 1 cut(s) 761
BstSCI CCNGG 1 cut(s) 389
BstSFI CTRYAG 4 cut(s) 103, 870, 1035, 1114
BstV1I GCAGC 8 cut(s) 75, 117, 237, 284, 329, 396, 856, 1294
BstV2I GAAGAC 1 cut(s) 540
BstXI CCANNNNNNTGG 1 cut(s) 673
BsuI GTATCC 1 cut(s) 1486
BsuRI GGCC 3 cut(s) 363, 518, 1361
BtgI CCRYGG 3 cut(s) 189, 1362, 1400
BtsCI GGATG 6 cut(s) 447, 813, 833, 967, 1392, 1396
BtsIMutI CAGTG 4 cut(s) 345, 571, 651, 1044
CaiI CAGNNNCTG 3 cut(s) 264, 390, 878
CciI TCATGA 1 cut(s) 423
CfoI GCGC 1 cut(s) 1162
Cfr13I GGNCC 3 cut(s) 193, 1066, 1228
Csp6I GTAC 4 cut(s) 397, 600, 710, 798
CviQI GTAC 4 cut(s) 397, 600, 710, 798
DdeI CTNAG 5 cut(s) 180, 636, 1026, 1220, 1379
DpnI GATC 4 cut(s) 233, 678, 1020, 1529
DpnII GATC 4 cut(s) 231, 676, 1018, 1527
EaeI YGGCCR 2 cut(s) 516, 1359
Ecl136II GAGCTC 1 cut(s) 1369
Eco130I CCWWGG 2 cut(s) 1362, 1400
Eco24I GRGCYC 2 cut(s) 288, 1371
Eco47I GGWCC 3 cut(s) 193, 1066, 1228
Eco53kI GAGCTC 1 cut(s) 1369
Eco57I CTGAAG 1 cut(s) 927
Eco88I CYCGRG 2 cut(s) 287, 1370
EcoICRI GAGCTC 1 cut(s) 1369
EcoNI CCTNNNNNAGG 1 cut(s) 1223
EcoRII CCWGG 1 cut(s) 389
EcoT14I CCWWGG 2 cut(s) 1362, 1400
EcoT22I ATGCAT 2 cut(s) 247, 1398
EcoT38I GRGCYC 2 cut(s) 288, 1371
ErhI CCWWGG 2 cut(s) 1362, 1400
FalI AAGNNNNNCTT 4 cut(s) 125, 157, 525, 557
FaqI GGGAC 2 cut(s) 749, 1389
FblI GTMKAC 2 cut(s) 405, 606
Fnu4HI GCNGC 8 cut(s) 64, 106, 226, 298, 343, 385, 870, 1283
FokI GGATG 6 cut(s) 434, 800, 820, 974, 1383, 1399
FriOI GRGCYC 2 cut(s) 288, 1371
Fsp4HI GCNGC 8 cut(s) 64, 106, 226, 298, 343, 385, 870, 1283
FspBI CTAG 2 cut(s) 773, 1196
GlaI GCGC 1 cut(s) 1161
GluI GCNGC 8 cut(s) 64, 106, 226, 298, 343, 385, 870, 1283
GsuI CTGGAG 2 cut(s) 903, 985
HaeII RGCGCY 1 cut(s) 1163
HaeIII GGCC 3 cut(s) 363, 518, 1361
HapII CCGG 1 cut(s) 1178
HhaI GCGC 1 cut(s) 1162
Hin6I GCGC 1 cut(s) 1160
HinP1I GCGC 1 cut(s) 1160
HincII GTYRAC 1 cut(s) 1095
HindII GTYRAC 1 cut(s) 1095
HindIII AAGCTT 2 cut(s) 734, 909
HinfI GANTC 4 cut(s) 325, 351, 576, 1418
HpaII CCGG 1 cut(s) 1178
HphI GGTGA 6 cut(s) 136, 442, 742, 781, 1255, 1463
Hpy166II GTNNAC 5 cut(s) 406, 607, 722, 945, 1095
Hpy188I TCNGA 4 cut(s) 151, 907, 988, 1018
Hpy188III TCNNGA 6 cut(s) 235, 277, 424, 658, 754, 1002
Hpy8I GTNNAC 5 cut(s) 406, 607, 722, 945, 1095
HpyAV CCTTC 8 cut(s) 305, 314, 421, 427, 541, 1150, 1345, 1377
HpyCH4III ACNGT 5 cut(s) 266, 604, 646, 1039, 1054
HpyCH4IV ACGT 1 cut(s) 1275
HpyF10VI GCNNNNNNNGC 3 cut(s) 63, 303, 878
HpyF3I CTNAG 5 cut(s) 180, 636, 1026, 1220, 1379
HpySE526I ACGT 1 cut(s) 1275
HspAI GCGC 1 cut(s) 1160
Kzo9I GATC 4 cut(s) 231, 676, 1018, 1527
LmnI GCTCC 2 cut(s) 392, 1366
Lsp1109I GCAGC 8 cut(s) 75, 117, 237, 284, 329, 396, 856, 1294
LweI GCATC 2 cut(s) 842, 1405
MaeI CTAG 2 cut(s) 773, 1196
MaeII ACGT 1 cut(s) 1275
MaeIII GTNAC 2 cut(s) 314, 787
MalI GATC 4 cut(s) 233, 678, 1020, 1529
MboI GATC 4 cut(s) 231, 676, 1018, 1527
MboII GAAGA 3 cut(s) 436, 517, 545
MfeI CAATTG 1 cut(s) 207
MhlI GDGCHC 3 cut(s) 288, 1371, 1443
MlsI TGGCCA 1 cut(s) 1361
MluCI AATT 3 cut(s) 207, 695, 852
MluNI TGGCCA 1 cut(s) 1361
MlyI GAGTC 1 cut(s) 345
MmeI TCCRAC 2 cut(s) 174, 861
Mox20I TGGCCA 1 cut(s) 1361
Mph1103I ATGCAT 2 cut(s) 247, 1398
MroXI GAANNNNTTC 1 cut(s) 443
MscI TGGCCA 1 cut(s) 1361
MseI TTAA 9 cut(s) 167, 377, 470, 543, 702, 860, 1263, 1287, 1407
MslI CAYNNNNRTG 4 cut(s) 428, 564, 933, 1482
Msp20I TGGCCA 1 cut(s) 1361
MspCI CTTAAG 2 cut(s) 542, 1286
MspI CCGG 1 cut(s) 1178
MspR9I CCNGG 1 cut(s) 391
MunI CAATTG 1 cut(s) 207
MvaI CCWGG 1 cut(s) 391
MwoI GCNNNNNNNGC 3 cut(s) 63, 303, 878
NcoI CCATGG 2 cut(s) 1362, 1400
NdeII GATC 4 cut(s) 231, 676, 1018, 1527
NlaIV GGNNCC 5 cut(s) 502, 997, 1011, 1068, 1523
NmuCI GTSAC 1 cut(s) 787
NsiI ATGCAT 2 cut(s) 247, 1398
NspI RCATGY 5 cut(s) 157, 225, 249, 720, 728
NspV TTCGAA 1 cut(s) 485
PaeR7I CTCGAG 1 cut(s) 1370
PagI TCATGA 1 cut(s) 423
PciI ACATGT 2 cut(s) 153, 724
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PdmI GAANNNNTTC 1 cut(s) 443
PfeI GAWTC 3 cut(s) 325, 576, 1418
PkrI GCNGC 8 cut(s) 65, 107, 227, 299, 344, 386, 871, 1284
PleI GAGTC 1 cut(s) 345
PpsI GAGTC 1 cut(s) 345
PscI ACATGT 2 cut(s) 153, 724
PshAI GACNNNNGTC 1 cut(s) 761
PshBI ATTAAT 2 cut(s) 377, 702
Psp124BI GAGCTC 1 cut(s) 1371
Psp1406I AACGTT 1 cut(s) 1275
Psp6I CCWGG 1 cut(s) 389
PspGI CCWGG 1 cut(s) 389
PspN4I GGNNCC 5 cut(s) 502, 997, 1011, 1068, 1523
PspPI GGNCC 3 cut(s) 193, 1066, 1228
PspXI VCTCGAGB 1 cut(s) 1370
PstI CTGCAG 2 cut(s) 107, 874
PstNI CAGNNNCTG 3 cut(s) 264, 390, 878
RsaI GTAC 4 cut(s) 398, 601, 711, 799
RsaNI GTAC 4 cut(s) 397, 600, 710, 798
RseI CAYNNNNRTG 4 cut(s) 428, 564, 933, 1482
SacI GAGCTC 1 cut(s) 1371
SaqAI TTAA 9 cut(s) 167, 377, 470, 543, 702, 860, 1263, 1287, 1407
SatI GCNGC 8 cut(s) 64, 106, 226, 298, 343, 385, 870, 1283
Sau3AI GATC 4 cut(s) 231, 676, 1018, 1527
Sau96I GGNCC 3 cut(s) 193, 1066, 1228
SchI GAGTC 1 cut(s) 345
ScrFI CCNGG 1 cut(s) 391
SduI GDGCHC 3 cut(s) 288, 1371, 1443
SfaNI GCATC 2 cut(s) 842, 1405
SfcI CTRYAG 4 cut(s) 103, 870, 1035, 1114
Sfr274I CTCGAG 1 cut(s) 1370
SfuI TTCGAA 1 cut(s) 485
SinI GGWCC 3 cut(s) 193, 1066, 1228
SlaI CTCGAG 1 cut(s) 1370
SmiMI CAYNNNNRTG 4 cut(s) 428, 564, 933, 1482
SmlI CTYRAG 3 cut(s) 542, 1286, 1370
SmoI CTYRAG 3 cut(s) 542, 1286, 1370
SpeI ACTAGT 1 cut(s) 772
Sse9I AATT 3 cut(s) 207, 695, 852
SspMI CTAG 2 cut(s) 773, 1196
SstI GAGCTC 1 cut(s) 1371
StyD4I CCNGG 1 cut(s) 389
StyI CCWWGG 2 cut(s) 1362, 1400
TaaI ACNGT 5 cut(s) 266, 604, 646, 1039, 1054
TaiI ACGT 1 cut(s) 1278
TaqI TCGA 8 cut(s) 198, 276, 485, 679, 753, 807, 1170, 1371
TaqII GACCGA 1 cut(s) 1245
TasI AATT 3 cut(s) 207, 695, 852
TatI WGTACW 2 cut(s) 396, 709
TfiI GAWTC 3 cut(s) 325, 576, 1418
Tru1I TTAA 9 cut(s) 167, 377, 470, 543, 702, 860, 1263, 1287, 1407
Tru9I TTAA 9 cut(s) 167, 377, 470, 543, 702, 860, 1263, 1287, 1407
TscAI CASTG 4 cut(s) 352, 571, 651, 1044
TseFI GTSAC 1 cut(s) 787
TseI GCWGC 8 cut(s) 63, 105, 225, 297, 342, 384, 869, 1282
Tsp45I GTSAC 1 cut(s) 787
TspDTI ATGAA 7 cut(s) 436, 440, 642, 688, 720, 1364, 1431
TspGWI ACGGA 2 cut(s) 545, 1002
TspRI CASTG 4 cut(s) 352, 571, 651, 1044
Vha464I CTTAAG 2 cut(s) 542, 1286
VpaK11BI GGWCC 3 cut(s) 193, 1066, 1228
VspI ATTAAT 2 cut(s) 377, 702
XagI CCTNNNNNAGG 1 cut(s) 1223
XapI RAATTY 1 cut(s) 852
XceI RCATGY 5 cut(s) 157, 225, 249, 720, 728
XcmI CCANNNNNNNNNTGG 1 cut(s) 1354
XhoI CTCGAG 1 cut(s) 1370
XmiI GTMKAC 2 cut(s) 405, 606
XmnI GAANNNNTTC 1 cut(s) 443
XspI CTAG 2 cut(s) 773, 1196
Zsp2I ATGCAT 2 cut(s) 247, 1398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.