Rroxscaffold_3G00269390

Partial alpha/beta-hydrolase lipase region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0011380
Physical Location & Seq
Forward (+)
62286010 .. 62289447
3438 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00269390.1

Sequence Viewer

Length: 2097 bp
ATGATGCAGCGACTCGTCGACAACGCCATCGCTGTCACCAAAGAGTCAGTGAAGACGTTTACGTATGAATCGCTGAACAATATTGTGAGATTTATCAATGGAGTTTCGGCTCTGCTGTTGGCGTTATTGCCAGGTAAGGCTAATATCCTTGAAGGCATACATGGCTGGGAGCTCAGGCCTACCTTTCGGGGACCTCGATTTCCTCGCTGGATGGAAAATGGGGTGTCCTCTTTCAATCAATTCATACATGAGCTTTCTGTGGATTCTGATTCCGAGGGTTCGAGCGTGAGCATAGACTATGGGGAAGAATATAGTGATGAAGACATATGTCCTGCGTCCCCATCTTCTCAAAGTTCAAGAGTCTCCAGGTCTTATAGTATGCCTAGGCAATGGACAGGATGGATGTGGATCAGATACATGTTTTATTGGATTCTGTTTCCTGCAAGGTTCCTGCTTGGCCTTCCGGGGCTTCTTTGCCGTTCTTGTTTTAATAGGAGCGCGACACCCTCTTCTGCACCAGGAAGCAGTCAGCTTTCGCACAGGACTAGTTCTTTGAATAGAGTGCAGTTCCTCAAGGATCACATTATTCAGCGCACTACTGATAGGAGACGTGGAGTCATTGAGGATCTTCATCTAGCGATTGAGATATTCATAGAAGCTGTATTCGATGTTGTTCATAAAGCAGCTCATTTTGTTCTTTCCCCATTTGATGCTCTTAGAACACTATTTGGGTGGTTTTTATCAAACAACAACAGAGTTCAAGATACTCATGGTATTGCTTCAGAAGCTTCTGTTCCCACAGCTACACTTGGAGATGATGATCCAGTTCCTACAGAAAGGAACACTTCGTTTCAGGCGCTAAATACTGATGCTCGAACATGCCAGGATGTCATTACAGAGCTTGGGTATCCATACGAAGCTATTCATGTGATCACTAGTGATGGATATGTTCTTCTCTTGGAAAGAATCCCAAGACGTGATTCACGAAAAGCTGTTTATCTACAACATGGAATTCTGGATTCATCTATGGGTTGGGTGTCCAATGGGGTTGTCGGTTCACCTGCATTTGCCGCCTATGATCAAGGATATGATGTCTTCCTCGGGAACTTTCGTGGCTTGGTTTCTAGGGAGCACATTGACAAAAGCATTTCCTCACGACAGTACTGGCAGTACTCAATCAATGAGCATGGGACTCAGGATATTCCTGCAATGATAGAGAAGATCCACCAAGTTAAAACTGCAGAATTGAAGCTTAGTCAACCTGATATTGAGGAAGAAACCAATGGTGATCAACCTTATAAGCTTTGTGCAATTTGCCATAGCTTGGGAGGAGCAGCTATATTAATGTATGTTGTAACACAGAGGATAGAAGAGAAACCCCACAGACTCTCGAGATTGATTTTATTATCACCTGCTGGCTTCCATGAAGATTCTACCTTTATGTTTTCGGTCGTGGAATACTCCTTTCTTCTATTGGCTCCCCTTCTGCAACCCCTTGTTCCTGGCATCTATATACCAACCAAATTCTTTCGCATGCTTCTAAACAAGTTGGCACGGGACTTCCATAATTACCCTGCTGTTGGAGGATTGGTTCAGACACTGATGAGTTATTTTGTAGGTGGAGATAGCTCAAATTGGGTTGGTGTGTTGGGCCTACCACATTATAACATGAATGACATGCCGGGGGTATCGTTCCAAGTGGCTCTCCATCTGGCACAAATGAAGCGGGCAAAAAGGTTTAGGATGTTTGATTATGGGAGAGCTTCTGCTAATATGGAGGTTTATGGATCTCCAGAGCCATTAGACTTGGGAGAGTACTATGGGCTCATTGATATTCCGGTTGATCTGGTTGCTGGACGTAAGGACAAGGTAATTAGGCCATCAATGGTAAAGAAGCATTATAAGGTGATGAAGAATTCAGGTGTAAATGCATCATACAATGAGTTTGAGTATGCACATTTGGACTTCACATTTTCCCACCGTGAAGAACTCTTGGCATATGTCATGTCACGCCTCTTGCTTGTGGAGCCGCCTCCAAAGCAGTCCAGCCCGAAGGGTCTAAAGCTAAAGAAGAAAGGACAGCAGGTTAATAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydro_lipase

Protein Analysis

698

Amino Acids

78.84

Weight (kDa)

6.9

Isoelectric Point (pI)

53.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydro_lipase PF04083 295 - 349 7.3e-15 Partial alpha/beta-hydrolase lipase region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011380)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18460 AT1G73920 AT1G73920
fragaria_vesca FvH4_5g12600 FvH4_5g12600
malus_domestica MD06G1212200.v1.1 MD14G1222700.v1.1
prunus_persica Prupe.5G218200_v2.0.a1
pyrus_communis pycom06g18940 pycom14g18440
rosa_chinensis RchiOBHm_Chr7g0184731
rosa_laevigata RLG00000004955
rosa_multiflora Rmu_ssc0000289.1_g000019
rosa_roxburghii Rroxscaffold_3G00269390
rosa_rugosa Rorug06G0464800
rosa_samantha Rh7AG078100 Rh7BG069200 Rh7CG070000 Rh7DG069400
rosa_wichuraiana Rw7G005730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1299, 1665, 1902
AarI CACCTGC 2 cut(s) 1069, 1420
Acc36I ACCTGC 3 cut(s) 1069, 1420, 2074
AccB7I CCANNNNNTGG 1 cut(s) 1324
AccI GTMKAC 1 cut(s) 18
AccII CGCG 1 cut(s) 500
AciI CCGC 3 cut(s) 1071, 1726, 2030
AclWI GGATC 6 cut(s) 416, 585, 633, 815, 1216, 1795
AcsI RAATTY 3 cut(s) 1011, 1523, 1915
AcuI CTGAAG 1 cut(s) 765
AfaI GTAC 3 cut(s) 1163, 1172, 1817
AfiI CCNNNNNNNGG 2 cut(s) 1324, 1580
AflIII ACRYGT 1 cut(s) 417
AgsI TTSAA 6 cut(s) 152, 235, 357, 556, 761, 1249
AhdI GACNNNNNGTC 1 cut(s) 614
AhlI ACTAGT 2 cut(s) 545, 935
AjiI CACGTC 2 cut(s) 611, 977
AjnI CCWGG 5 cut(s) 130, 365, 517, 882, 1501
AjuI GAANNNNNNNTTGG 2 cut(s) 964, 996
AloI GAACNNNNNNTCC 2 cut(s) 1575, 1607
Alw21I GWGCWC 2 cut(s) 174, 1134
Alw26I GTCTC 2 cut(s) 367, 601
AlwI GGATC 6 cut(s) 416, 585, 633, 815, 1216, 1795
AlwNI CAGNNNCTG 1 cut(s) 1600
Ama87I CYCGRG 2 cut(s) 1100, 1390
AoxI GGCC 4 cut(s) 176, 457, 1651, 1877
ApeKI GCWGC 3 cut(s) 7, 683, 1334
ApoI RAATTY 3 cut(s) 1011, 1523, 1915
ArsI GACNNNNNNTTYG 2 cut(s) 535, 567
AseI ATTAAT 1 cut(s) 1343
Asp700I GAANNNNTTC 1 cut(s) 921
AspA2I CCTAGG 1 cut(s) 383
AspLEI GCGC 3 cut(s) 500, 594, 859
AspS9I GGNCC 2 cut(s) 191, 1651
AsuC2I CCSGG 2 cut(s) 465, 1683
AsuHPI GGTGA 5 cut(s) 28, 1050, 1298, 1401, 1918
AvaI CYCGRG 2 cut(s) 1100, 1390
AvaII GGWCC 1 cut(s) 191
AvrII CCTAGG 1 cut(s) 383
BanII GRGCYC 2 cut(s) 174, 1827
BbsI GAAGAC 3 cut(s) 59, 327, 1087
Bbv12I GWGCWC 2 cut(s) 174, 1134
BbvI GCAGC 3 cut(s) 19, 695, 1346
BccI CCATC 7 cut(s) 35, 205, 349, 393, 935, 1716, 1888
BceAI ACGGC 1 cut(s) 462
BciT130I CCWGG 5 cut(s) 132, 367, 519, 884, 1503
BciVI GTATCC 1 cut(s) 918
BclI TGATCA 3 cut(s) 930, 1078, 1288
BcnI CCSGG 2 cut(s) 465, 1683
BcoDI GTCTC 2 cut(s) 367, 601
BcuI ACTAGT 2 cut(s) 545, 935
BfaI CTAG 5 cut(s) 384, 546, 635, 936, 1125
BfmI CTRYAG 2 cut(s) 831, 1239
BfoI RGCGCY 1 cut(s) 860
BfuAI ACCTGC 3 cut(s) 1069, 1420, 2074
BfuI GTATCC 1 cut(s) 918
BisI GCNGC 5 cut(s) 8, 684, 1071, 1335, 2030
BlnI CCTAGG 1 cut(s) 383
BlsI GCNGC 5 cut(s) 9, 685, 1072, 1336, 2031
BmcAI AGTACT 3 cut(s) 1163, 1172, 1817
Bme1390I CCNGG 7 cut(s) 132, 367, 465, 519, 884, 1503, 1683
Bme18I GGWCC 1 cut(s) 191
BmeRI GACNNNNNGTC 1 cut(s) 614
BmeT110I CYCGRG 2 cut(s) 1100, 1390
BmgBI CACGTC 2 cut(s) 611, 977
BmgT120I GGNCC 2 cut(s) 191, 1651
BmiI GGNNCC 4 cut(s) 192, 449, 1479, 2028
BmrFI CCNGG 7 cut(s) 132, 367, 465, 519, 884, 1503, 1683
BmsI GCATC 4 cut(s) 700, 859, 1515, 1940
BoxI GACNNNNGTC 1 cut(s) 327
BpiI GAAGAC 3 cut(s) 59, 327, 1087
BpmI CTGGAG 2 cut(s) 349, 1776
Bpu10I CCTNAGC 1 cut(s) 173
BpuEI CTTGAG 1 cut(s) 557
BpuMI CCSGG 2 cut(s) 465, 1683
BsaAI YACGTR 1 cut(s) 63
BsaBI GATNNNNATC 3 cut(s) 407, 630, 819
BsaJI CCNNGG 5 cut(s) 273, 383, 464, 1099, 1682
BsaWI WCCGGW 1 cut(s) 1837
BsaXI ACNNNNNCTCC 2 cut(s) 1575, 1605
Bsc4I CCNNNNNNNGG 2 cut(s) 1324, 1580
Bse1I ACTGG 2 cut(s) 824, 1169
Bse3DI GCAATG 2 cut(s) 395, 1215
Bse8I GATNNNNATC 3 cut(s) 407, 630, 819
BseBI CCWGG 5 cut(s) 132, 367, 519, 884, 1503
BseDI CCNNGG 5 cut(s) 273, 383, 464, 1099, 1682
BseGI GGATG 5 cut(s) 216, 404, 408, 892, 1749
BseJI GATNNNNATC 3 cut(s) 407, 630, 819
BseLI CCNNNNNNNGG 2 cut(s) 1324, 1580
BseMI GCAATG 2 cut(s) 395, 1215
BseMII CTCAG 2 cut(s) 187, 1208
BseNI ACTGG 2 cut(s) 824, 1169
BseRI GAGGAG 1 cut(s) 1344
BseXI GCAGC 3 cut(s) 19, 695, 1346
BseYI CCCAGC 1 cut(s) 165
BsgI GTGCAG 2 cut(s) 498, 584
Bsh1236I CGCG 1 cut(s) 500
Bsh1285I CGRYCG 1 cut(s) 1452
BshFI GGCC 4 cut(s) 178, 459, 1653, 1879
BsiEI CGRYCG 1 cut(s) 1452
BsiHKAI GWGCWC 2 cut(s) 174, 1134
BsiHKCI CYCGRG 2 cut(s) 1100, 1390
BsiSI CCGG 3 cut(s) 464, 1682, 1838
BslFI GGGAC 4 cut(s) 204, 322, 1204, 1571
BslI CCNNNNNNNGG 2 cut(s) 1324, 1580
BsmAI GTCTC 2 cut(s) 367, 601
BsmBI CGTCTC 1 cut(s) 601
BsmFI GGGAC 4 cut(s) 204, 322, 1204, 1571
BsnI GGCC 4 cut(s) 178, 459, 1653, 1879
BsoBI CYCGRG 2 cut(s) 1100, 1390
Bsp1286I GDGCHC 3 cut(s) 174, 1134, 1827
BspACI CCGC 3 cut(s) 1071, 1726, 2030
BspANI GGCC 4 cut(s) 178, 459, 1653, 1879
BspCNI CTCAG 2 cut(s) 186, 1207
BspFNI CGCG 1 cut(s) 500
BspLI GGNNCC 4 cut(s) 192, 449, 1479, 2028
BspMAI CTGCAG 1 cut(s) 1243
BspMI ACCTGC 3 cut(s) 1069, 1420, 2074
BspPI GGATC 6 cut(s) 416, 585, 633, 815, 1216, 1795
BsrDI GCAATG 2 cut(s) 395, 1215
BsrI ACTGG 2 cut(s) 824, 1169
BssECI CCNNGG 5 cut(s) 273, 383, 464, 1099, 1682
BssT1I CCWWGG 1 cut(s) 383
Bst2UI CCWGG 5 cut(s) 132, 367, 519, 884, 1503
Bst4CI ACNGT 2 cut(s) 1161, 1982
Bst6I CTCTTC 2 cut(s) 514, 1365
BstBAI YACGTR 1 cut(s) 63
BstC8I GCNNGC 3 cut(s) 1417, 1535, 1728
BstDEI CTNAG 4 cut(s) 173, 716, 1194, 1253
BstF5I GGATG 5 cut(s) 216, 404, 408, 892, 1749
BstFNI CGCG 1 cut(s) 500
BstH2I RGCGCY 1 cut(s) 860
BstHHI GCGC 3 cut(s) 500, 594, 859
BstMAI GTCTC 2 cut(s) 367, 601
BstMCI CGRYCG 1 cut(s) 1452
BstMWI GCNNNNNNNGC 5 cut(s) 162, 785, 1070, 2026, 2038
BstNI CCWGG 5 cut(s) 132, 367, 519, 884, 1503
BstNSI RCATGY 4 cut(s) 421, 882, 1537, 1681
BstPAI GACNNNNGTC 1 cut(s) 327
BstSCI CCNGG 7 cut(s) 130, 365, 463, 517, 882, 1501, 1681
BstSFI CTRYAG 2 cut(s) 831, 1239
BstSNI TACGTA 1 cut(s) 63
BstUI CGCG 1 cut(s) 500
BstV1I GCAGC 3 cut(s) 19, 695, 1346
BstV2I GAAGAC 3 cut(s) 59, 327, 1087
BstX2I RGATCY 3 cut(s) 625, 1221, 1787
BstYI RGATCY 3 cut(s) 625, 1221, 1787
BsuI GTATCC 1 cut(s) 918
BsuRI GGCC 4 cut(s) 178, 459, 1653, 1879
BtgZI GCGATG 1 cut(s) 13
BtrI CACGTC 2 cut(s) 611, 977
BtsCI GGATG 5 cut(s) 216, 404, 408, 892, 1749
BtsIMutI CAGTG 2 cut(s) 54, 1598
BveI ACCTGC 3 cut(s) 1069, 1420, 2074
Cac8I GCNNGC 3 cut(s) 1417, 1535, 1728
CaiI CAGNNNCTG 1 cut(s) 1600
CfoI GCGC 3 cut(s) 500, 594, 859
Cfr13I GGNCC 2 cut(s) 191, 1651
CseI GACGC 1 cut(s) 324
Csp6I GTAC 3 cut(s) 1162, 1171, 1816
CviQI GTAC 3 cut(s) 1162, 1171, 1816
DdeI CTNAG 4 cut(s) 173, 716, 1194, 1253
DriI GACNNNNNGTC 1 cut(s) 614
Eam1104I CTCTTC 2 cut(s) 514, 1365
Eam1105I GACNNNNNGTC 1 cut(s) 614
EarI CTCTTC 2 cut(s) 514, 1365
Ecl136II GAGCTC 1 cut(s) 172
Eco105I TACGTA 1 cut(s) 63
Eco130I CCWWGG 1 cut(s) 383
Eco147I AGGCCT 1 cut(s) 178
Eco24I GRGCYC 2 cut(s) 174, 1827
Eco47I GGWCC 1 cut(s) 191
Eco53kI GAGCTC 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 765
Eco88I CYCGRG 2 cut(s) 1100, 1390
EcoICRI GAGCTC 1 cut(s) 172
EcoO109I RGGNCCY 1 cut(s) 191
EcoRI GAATTC 2 cut(s) 1011, 1915
EcoRII CCWGG 5 cut(s) 130, 365, 517, 882, 1501
EcoT14I CCWWGG 1 cut(s) 383
EcoT22I ATGCAT 1 cut(s) 1933
EcoT38I GRGCYC 2 cut(s) 174, 1827
ErhI CCWWGG 1 cut(s) 383
Esp3I CGTCTC 1 cut(s) 601
FalI AAGNNNNNCTT 2 cut(s) 829, 861
FaqI GGGAC 4 cut(s) 204, 322, 1204, 1571
FauI CCCGC 1 cut(s) 1719
FauNDI CATATG 2 cut(s) 326, 1999
FbaI TGATCA 3 cut(s) 930, 1078, 1288
FblI GTMKAC 1 cut(s) 18
Fnu4HI GCNGC 5 cut(s) 8, 684, 1071, 1335, 2030
FokI GGATG 5 cut(s) 223, 411, 415, 899, 1756
FriOI GRGCYC 2 cut(s) 174, 1827
Fsp4HI GCNGC 5 cut(s) 8, 684, 1071, 1335, 2030
FspBI CTAG 5 cut(s) 384, 546, 635, 936, 1125
GlaI GCGC 3 cut(s) 499, 593, 858
GluI GCNGC 5 cut(s) 8, 684, 1071, 1335, 2030
GsaI CCCAGC 1 cut(s) 169
GsuI CTGGAG 2 cut(s) 349, 1776
HaeII RGCGCY 1 cut(s) 860
HaeIII GGCC 4 cut(s) 178, 459, 1653, 1879
HapII CCGG 3 cut(s) 464, 1682, 1838
HgaI GACGC 1 cut(s) 324
HhaI GCGC 3 cut(s) 500, 594, 859
Hin6I GCGC 3 cut(s) 498, 592, 857
HinP1I GCGC 3 cut(s) 498, 592, 857
HincII GTYRAC 2 cut(s) 19, 1259
HindII GTYRAC 2 cut(s) 19, 1259
HindIII AAGCTT 3 cut(s) 786, 1250, 1301
HpaII CCGG 3 cut(s) 464, 1682, 1838
HphI GGTGA 5 cut(s) 28, 1050, 1298, 1401, 1918
Hpy166II GTNNAC 4 cut(s) 19, 60, 1058, 1259
Hpy188I TCNGA 5 cut(s) 268, 274, 413, 784, 1596
Hpy8I GTNNAC 4 cut(s) 19, 60, 1058, 1259
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 4 cut(s) 146, 470, 1493, 2047
HpyCH4III ACNGT 2 cut(s) 1161, 1982
HpyCH4IV ACGT 5 cut(s) 56, 62, 610, 976, 1858
HpyF10VI GCNNNNNNNGC 5 cut(s) 162, 785, 1070, 2026, 2038
HpyF3I CTNAG 4 cut(s) 173, 716, 1194, 1253
HpySE526I ACGT 5 cut(s) 56, 62, 610, 976, 1858
HspAI GCGC 3 cut(s) 498, 592, 857
Ksp22I TGATCA 3 cut(s) 930, 1078, 1288
LmnI GCTCC 6 cut(s) 169, 495, 1129, 1331, 1483, 2026
Lsp1109I GCAGC 3 cut(s) 19, 695, 1346
LweI GCATC 4 cut(s) 700, 859, 1515, 1940
MaeI CTAG 5 cut(s) 384, 546, 635, 936, 1125
MaeII ACGT 5 cut(s) 56, 62, 610, 976, 1858
MaeIII GTNAC 3 cut(s) 34, 1354, 2007
MflI RGATCY 3 cut(s) 625, 1221, 1787
MhlI GDGCHC 3 cut(s) 174, 1134, 1827
MluCI AATT 9 cut(s) 239, 1011, 1244, 1311, 1523, 1567, 1633, 1872, 1915
MlyI GAGTC 6 cut(s) 6, 53, 369, 624, 1186, 1380
MmeI TCCRAC 1 cut(s) 1561
Mph1103I ATGCAT 1 cut(s) 1933
MroXI GAANNNNTTC 1 cut(s) 921
MseI TTAA 5 cut(s) 489, 1233, 1343, 2088, 2095
MspI CCGG 3 cut(s) 464, 1682, 1838
MspR9I CCNGG 7 cut(s) 132, 367, 465, 519, 884, 1503, 1683
MvaI CCWGG 5 cut(s) 132, 367, 519, 884, 1503
MvnI CGCG 1 cut(s) 500
MwoI GCNNNNNNNGC 5 cut(s) 162, 785, 1070, 2026, 2038
NciI CCSGG 2 cut(s) 465, 1683
NdeI CATATG 2 cut(s) 326, 1999
NlaIV GGNNCC 4 cut(s) 192, 449, 1479, 2028
NmuCI GTSAC 2 cut(s) 34, 2007
NsiI ATGCAT 1 cut(s) 1933
NspI RCATGY 4 cut(s) 421, 882, 1537, 1681
PaeI GCATGC 1 cut(s) 1537
PaeR7I CTCGAG 1 cut(s) 1390
PaqCI CACCTGC 2 cut(s) 1069, 1420
PceI AGGCCT 1 cut(s) 178
PciI ACATGT 1 cut(s) 417
PcsI WCGNNNNNNNCGW 2 cut(s) 193, 982
PdmI GAANNNNTTC 1 cut(s) 921
PfeI GAWTC 8 cut(s) 68, 263, 269, 430, 966, 980, 1019, 1430
PflMI CCANNNNNTGG 1 cut(s) 1324
PkrI GCNGC 5 cut(s) 9, 685, 1072, 1336, 2031
PleI GAGTC 6 cut(s) 6, 52, 368, 623, 1186, 1380
PpsI GAGTC 6 cut(s) 6, 52, 368, 623, 1186, 1380
Ppu21I YACGTR 1 cut(s) 63
PpuMI RGGWCCY 1 cut(s) 191
PscI ACATGT 1 cut(s) 417
PshAI GACNNNNGTC 1 cut(s) 327
PshBI ATTAAT 1 cut(s) 1343
PsiI TTATAA 3 cut(s) 1299, 1665, 1902
Psp124BI GAGCTC 1 cut(s) 174
Psp5II RGGWCCY 1 cut(s) 191
Psp6I CCWGG 5 cut(s) 130, 365, 517, 882, 1501
PspFI CCCAGC 1 cut(s) 165
PspGI CCWGG 5 cut(s) 130, 365, 517, 882, 1501
PspN4I GGNNCC 4 cut(s) 192, 449, 1479, 2028
PspPI GGNCC 2 cut(s) 191, 1651
PspPPI RGGWCCY 1 cut(s) 191
PstI CTGCAG 1 cut(s) 1243
PstNI CAGNNNCTG 1 cut(s) 1600
PsuI RGATCY 3 cut(s) 625, 1221, 1787
RsaI GTAC 3 cut(s) 1163, 1172, 1817
RsaNI GTAC 3 cut(s) 1162, 1171, 1816
SacI GAGCTC 1 cut(s) 174
SalI GTCGAC 1 cut(s) 17
SaqAI TTAA 5 cut(s) 489, 1233, 1343, 2088, 2095
SatI GCNGC 5 cut(s) 8, 684, 1071, 1335, 2030
Sau96I GGNCC 2 cut(s) 191, 1651
ScaI AGTACT 3 cut(s) 1163, 1172, 1817
SchI GAGTC 6 cut(s) 6, 53, 369, 624, 1186, 1380
ScrFI CCNGG 7 cut(s) 132, 367, 465, 519, 884, 1503, 1683
SduI GDGCHC 3 cut(s) 174, 1134, 1827
SfaNI GCATC 4 cut(s) 700, 859, 1515, 1940
SfcI CTRYAG 2 cut(s) 831, 1239
Sfr274I CTCGAG 1 cut(s) 1390
SinI GGWCC 1 cut(s) 191
SlaI CTCGAG 1 cut(s) 1390
SmlI CTYRAG 2 cut(s) 572, 1390
SmoI CTYRAG 2 cut(s) 572, 1390
SnaBI TACGTA 1 cut(s) 63
SpeI ACTAGT 2 cut(s) 545, 935
SphI GCATGC 1 cut(s) 1537
Sse9I AATT 9 cut(s) 239, 1011, 1244, 1311, 1523, 1567, 1633, 1872, 1915
SseBI AGGCCT 1 cut(s) 178
SsiI CCGC 3 cut(s) 1071, 1726, 2030
SspI AATATT 1 cut(s) 82
SspMI CTAG 5 cut(s) 384, 546, 635, 936, 1125
SstI GAGCTC 1 cut(s) 174
StuI AGGCCT 1 cut(s) 178
StyD4I CCNGG 7 cut(s) 130, 365, 463, 517, 882, 1501, 1681
StyI CCWWGG 1 cut(s) 383
TaaI ACNGT 2 cut(s) 1161, 1982
TaiI ACGT 5 cut(s) 59, 65, 613, 979, 1861
TaqI TCGA 6 cut(s) 18, 196, 281, 666, 874, 1391
TaqII GACCGA 1 cut(s) 1438
TasI AATT 9 cut(s) 239, 1011, 1244, 1311, 1523, 1567, 1633, 1872, 1915
TatI WGTACW 3 cut(s) 1161, 1170, 1815
TauI GCSGC 2 cut(s) 1073, 2032
TfiI GAWTC 8 cut(s) 68, 263, 269, 430, 966, 980, 1019, 1430
Tru1I TTAA 5 cut(s) 489, 1233, 1343, 2088, 2095
Tru9I TTAA 5 cut(s) 489, 1233, 1343, 2088, 2095
TscAI CASTG 2 cut(s) 54, 1605
TseFI GTSAC 2 cut(s) 34, 2007
TseI GCWGC 3 cut(s) 7, 683, 1334
Tsp45I GTSAC 2 cut(s) 34, 2007
TspRI CASTG 2 cut(s) 54, 1605
Van91I CCANNNNNTGG 1 cut(s) 1324
VpaK11BI GGWCC 1 cut(s) 191
VspI ATTAAT 1 cut(s) 1343
XapI RAATTY 3 cut(s) 1011, 1523, 1915
XceI RCATGY 4 cut(s) 421, 882, 1537, 1681
XhoI CTCGAG 1 cut(s) 1390
XmaJI CCTAGG 1 cut(s) 383
XmiI GTMKAC 1 cut(s) 18
XmnI GAANNNNTTC 1 cut(s) 921
XspI CTAG 5 cut(s) 384, 546, 635, 936, 1125
ZrmI AGTACT 3 cut(s) 1163, 1172, 1817
Zsp2I ATGCAT 1 cut(s) 1933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.