FvH4_5g14750

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0018043
Physical Location & Seq
Reverse (-)
8379696 .. 8380946
1251 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14750.t1

Sequence Viewer

Length: 1251 bp
ATGGAAAAGTATCGTCCCGATTTGGAGACAGTTTTAGAGGTTCCTCACAAGAACACTACAAAGCCAAGGAACAGAAAGAAATCGGACACAATTGGAGAGGTCCCTCGCAAGAAGACAACAACAAAGCTATGGAACAGAATGAAATCATCGATTGAATCAAAAAGATCAGTAAAGAAATCAACGTCAATGGCTAGCGATGGCACGACACTAGCTAGCTTACTTGAAGTTATTGGTGCCCCCTTGACTCCAGCTACTCTTTGTTTCAACAACGATGGTCACCAACCCCTCGCCAGGGCTAAATACATTGTGAAGCAGTACGTAGCGGCGGTGGGCGGGGAGAAGGCTTTGTCATCATCGGACAACTTATTTGCGACAGGCAATGTGAAGATCACGATGACAAACAGGACTGGAAGCTCGAAGTCCATTAGCGGTGGCAGCGGACATGGAATGCCGGGGTTGCTTTGGCAAAAGGGGGAGCTATGGTGCTTGGACCTAATGTTGTCTCCTGTCCACAAATTAGTTAGCACGGGGAGTGATGGCAAGACGCTTTTGCCTTGGCATTACTCCCACGCGTATCGAGTACCGCCTCTTCCCCTTGGACCTCTCCTTCAGGGTCTTAATCCGAGGATAACAGCAAATTTATTCAGCAAGTCAGATTGGTTTGCGGACAAATCAATAAACAAAGAGGACTGCTTCATACTTGAACTGATGGTTGAACCTTCAAAACTAAGCAGCAGCAACAACGTAGAGACAGTGCGACACACCATTTTGGGCTACTTCAGCCAGAAAACAGGCCTCTTGGTGAAGCTAGAAGACTCTCGTCGGCTAAGGTTGACAGCTTTGGGAAAGCATAGTGACTGGGAATCAACAATGGAGTCATACTTCCAAGATTACAGAGCCATCGACGGCGTGAACATTGCACACAGGGTCAGTACTTGTGTGTCGTTGCGTGAGGAAAGCTCGGAAGGCCATTCTCTGAGAAGAAAAGTGAAATTGGTTTGGGAACTTGAAGATGTAGGTTTGGACGTCAAGGCGTTGTCCATGGAGTGGTTCTTGCCTCGTTGTGATATGAAAACAGAGGAAGAACGACGTGGGTTGGTGACGTCTAATTCTGGTGCAAAATCAACATCCAGACTAGTATCTCCTTCTATGAGATTTGAAGCTAGCAAAATAGTACCCATTGATGACTCCGATGAAGATCTAGAAAGTTGTGACATTAGTGACCAGGAGGATGACTTCGACAATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

417

Amino Acids

46.43

Weight (kDa)

8.37

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 123 - 353 2e-59 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018043)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g14750
rosa_chinensis RchiOBHm_Chr1g0369021
rosa_laevigata RLG00000027164
rosa_multiflora Rmu_sc0004250.1_g000030
rosa_roxburghii Rroxscaffold_4G00288080
rosa_rugosa Rorug01G0347000
rosa_samantha Rh1AG353900 Rh1BG317400 Rh1CG331900 Rh1DG348300
rosa_wichuraiana Rw1G031270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 1029, 1106
AccB1I GGYRCC 1 cut(s) 233
AccB7I CCANNNNNTGG 1 cut(s) 1047
AccII CGCG 1 cut(s) 572
AciI CCGC 7 cut(s) 323, 326, 333, 429, 438, 584, 665
AcsI RAATTY 1 cut(s) 637
AcuI CTGAAG 2 cut(s) 593, 763
AcyI GRCGYC 2 cut(s) 1026, 1103
AfaI GTAC 4 cut(s) 317, 582, 934, 1176
AfiI CCNNNNNNNGG 3 cut(s) 291, 292, 1047
AflIII ACRYGT 1 cut(s) 570
AgsI TTSAA 8 cut(s) 155, 224, 265, 704, 716, 723, 1010, 1160
AhlI ACTAGT 1 cut(s) 1135
AjiI CACGTC 1 cut(s) 1091
AjnI CCWGG 2 cut(s) 290, 1224
Alw26I GTCTC 3 cut(s) 20, 507, 743
AoxI GGCC 2 cut(s) 793, 967
ApeKI GCWGC 3 cut(s) 435, 732, 735
ApoI RAATTY 1 cut(s) 637
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
AspS9I GGNCC 3 cut(s) 100, 490, 599
AsuC2I CCSGG 1 cut(s) 453
AsuHPI GGTGA 3 cut(s) 269, 814, 1111
AsuNHI GCTAGC 3 cut(s) 191, 212, 1163
AvaII GGWCC 3 cut(s) 100, 490, 599
BaeGI GKGCMC 1 cut(s) 238
BanI GGYRCC 1 cut(s) 233
BarI GAAGNNNNNNTAC 2 cut(s) 573, 605
BbsI GAAGAC 2 cut(s) 119, 819
BbvI GCAGC 3 cut(s) 447, 744, 747
BccI CCATC 5 cut(s) 191, 266, 530, 703, 908
BceAI ACGGC 1 cut(s) 922
BciT130I CCWGG 2 cut(s) 292, 1226
BcnI CCSGG 1 cut(s) 453
BcoDI GTCTC 3 cut(s) 20, 507, 743
BcuI ACTAGT 1 cut(s) 1135
BfaI CTAG 7 cut(s) 192, 209, 213, 809, 1136, 1164, 1202
BglII AGATCT 1 cut(s) 1198
BisI GCNGC 4 cut(s) 324, 436, 733, 736
BlsI GCNGC 4 cut(s) 325, 437, 734, 737
BmcAI AGTACT 1 cut(s) 934
Bme1390I CCNGG 3 cut(s) 292, 453, 1226
Bme18I GGWCC 3 cut(s) 100, 490, 599
BmgBI CACGTC 1 cut(s) 1091
BmgT120I GGNCC 3 cut(s) 100, 490, 599
BmiI GGNNCC 3 cut(s) 42, 102, 235
BmrFI CCNGG 3 cut(s) 292, 453, 1226
BmrI ACTGGG 1 cut(s) 868
BmtI GCTAGC 3 cut(s) 195, 216, 1167
BmuI ACTGGG 1 cut(s) 868
BoxI GACNNNNGTC 1 cut(s) 819
BpiI GAAGAC 2 cut(s) 119, 819
BplI GAGNNNNNCTC 2 cut(s) 944, 976
BpmI CTGGAG 1 cut(s) 231
Bpu10I CCTNAGC 1 cut(s) 827
BpuMI CCSGG 1 cut(s) 453
Bsa29I ATCGAT 1 cut(s) 149
BsaAI YACGTR 1 cut(s) 319
BsaBI GATNNNNATC 1 cut(s) 1197
BsaHI GRCGYC 2 cut(s) 1026, 1103
BsaJI CCNNGG 7 cut(s) 65, 291, 452, 554, 595, 623, 1041
BsaXI ACNNNNNCTCC 2 cut(s) 467, 497
Bsc4I CCNNNNNNNGG 3 cut(s) 291, 292, 1047
Bse1I ACTGG 2 cut(s) 412, 863
Bse3DI GCAATG 2 cut(s) 385, 915
Bse8I GATNNNNATC 1 cut(s) 1197
BseBI CCWGG 2 cut(s) 292, 1226
BseCI ATCGAT 1 cut(s) 149
BseDI CCNNGG 7 cut(s) 65, 291, 452, 554, 595, 623, 1041
BseGI GGATG 2 cut(s) 1127, 1237
BseJI GATNNNNATC 1 cut(s) 1197
BseLI CCNNNNNNNGG 3 cut(s) 291, 292, 1047
BseMI GCAATG 2 cut(s) 385, 915
BseMII CTCAG 1 cut(s) 968
BseNI ACTGG 2 cut(s) 412, 863
BseSI GKGCMC 1 cut(s) 238
BseXI GCAGC 3 cut(s) 447, 744, 747
Bsh1236I CGCG 1 cut(s) 572
BshFI GGCC 2 cut(s) 795, 969
BshNI GGYRCC 1 cut(s) 233
BshVI ATCGAT 1 cut(s) 149
BsiSI CCGG 1 cut(s) 452
BslFI GGGAC 1 cut(s) 86
BslI CCNNNNNNNGG 3 cut(s) 291, 292, 1047
BsmAI GTCTC 3 cut(s) 20, 507, 743
BsmFI GGGAC 1 cut(s) 86
BsmI GAATGC 1 cut(s) 453
BsnI GGCC 2 cut(s) 795, 969
Bsp1286I GDGCHC 1 cut(s) 238
Bsp143I GATC 3 cut(s) 164, 387, 1198
Bsp19I CCATGG 1 cut(s) 1041
BspACI CCGC 7 cut(s) 323, 326, 333, 429, 438, 584, 665
BspANI GGCC 2 cut(s) 795, 969
BspCNI CTCAG 1 cut(s) 969
BspDI ATCGAT 1 cut(s) 149
BspFNI CGCG 1 cut(s) 572
BspLI GGNNCC 3 cut(s) 42, 102, 235
BspOI GCTAGC 3 cut(s) 195, 216, 1167
BspT107I GGYRCC 1 cut(s) 233
BsrDI GCAATG 2 cut(s) 385, 915
BsrI ACTGG 2 cut(s) 412, 863
BssECI CCNNGG 7 cut(s) 65, 291, 452, 554, 595, 623, 1041
BssMI GATC 3 cut(s) 164, 387, 1198
BssNI GRCGYC 2 cut(s) 1026, 1103
BssT1I CCWWGG 4 cut(s) 65, 554, 595, 1041
Bst2UI CCWGG 2 cut(s) 292, 1226
Bst4CI ACNGT 2 cut(s) 31, 754
Bst6I CTCTTC 1 cut(s) 594
BstACI GRCGYC 2 cut(s) 1026, 1103
BstBAI YACGTR 1 cut(s) 319
BstC8I GCNNGC 3 cut(s) 193, 214, 1165
BstDEI CTNAG 3 cut(s) 728, 827, 977
BstDSI CCRYGG 1 cut(s) 1041
BstEII GGTNACC 1 cut(s) 275
BstF5I GGATG 2 cut(s) 1127, 1237
BstFNI CGCG 1 cut(s) 572
BstKTI GATC 3 cut(s) 167, 390, 1201
BstMAI GTCTC 3 cut(s) 20, 507, 743
BstMBI GATC 3 cut(s) 164, 387, 1198
BstMWI GCNNNNNNNGC 4 cut(s) 435, 457, 780, 966
BstNI CCWGG 2 cut(s) 292, 1226
BstPAI GACNNNNGTC 1 cut(s) 819
BstPI GGTNACC 1 cut(s) 275
BstSCI CCNGG 3 cut(s) 290, 451, 1224
BstSLI GKGCMC 1 cut(s) 238
BstSNI TACGTA 1 cut(s) 319
BstUI CGCG 1 cut(s) 572
BstV1I GCAGC 3 cut(s) 447, 744, 747
BstV2I GAAGAC 2 cut(s) 119, 819
BstX2I RGATCY 1 cut(s) 1198
BstYI RGATCY 1 cut(s) 1198
Bsu15I ATCGAT 1 cut(s) 149
BsuRI GGCC 2 cut(s) 795, 969
BsuTUI ATCGAT 1 cut(s) 149
BtgI CCRYGG 1 cut(s) 1041
BtgZI GCGATG 1 cut(s) 210
BtrI CACGTC 1 cut(s) 1091
BtsCI GGATG 2 cut(s) 1127, 1237
BtsIMutI CAGTG 1 cut(s) 759
Cac8I GCNNGC 3 cut(s) 193, 214, 1165
Cfr13I GGNCC 3 cut(s) 100, 490, 599
ClaI ATCGAT 1 cut(s) 149
CseI GACGC 1 cut(s) 553
Csp6I GTAC 4 cut(s) 316, 581, 933, 1175
CviAII CATG 2 cut(s) 443, 1042
CviQI GTAC 4 cut(s) 316, 581, 933, 1175
DdeI CTNAG 3 cut(s) 728, 827, 977
DpnI GATC 3 cut(s) 166, 389, 1200
DpnII GATC 3 cut(s) 164, 387, 1198
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
Eco105I TACGTA 1 cut(s) 319
Eco130I CCWWGG 4 cut(s) 65, 554, 595, 1041
Eco147I AGGCCT 1 cut(s) 795
Eco47I GGWCC 3 cut(s) 100, 490, 599
Eco57I CTGAAG 2 cut(s) 593, 763
Eco91I GGTNACC 1 cut(s) 275
EcoO109I RGGNCCY 1 cut(s) 100
EcoO65I GGTNACC 1 cut(s) 275
EcoRII CCWGG 2 cut(s) 290, 1224
EcoT14I CCWWGG 4 cut(s) 65, 554, 595, 1041
ErhI CCWWGG 4 cut(s) 65, 554, 595, 1041
FaeI CATG 2 cut(s) 446, 1045
FaiI YATR 9 cut(s) 130, 444, 481, 698, 852, 880, 1043, 1070, 1151
FaqI GGGAC 1 cut(s) 86
FatI CATG 2 cut(s) 442, 1041
FauI CCCGC 1 cut(s) 326
Fnu4HI GCNGC 4 cut(s) 324, 436, 733, 736
FokI GGATG 2 cut(s) 1114, 1244
Fsp4HI GCNGC 4 cut(s) 324, 436, 733, 736
FspBI CTAG 7 cut(s) 192, 209, 213, 809, 1136, 1164, 1202
GluI GCNGC 4 cut(s) 324, 436, 733, 736
GsuI CTGGAG 1 cut(s) 231
HaeIII GGCC 2 cut(s) 795, 969
HapII CCGG 1 cut(s) 452
HgaI GACGC 1 cut(s) 553
Hin1I GRCGYC 2 cut(s) 1026, 1103
Hin1II CATG 2 cut(s) 446, 1045
HincII GTYRAC 1 cut(s) 834
HindII GTYRAC 1 cut(s) 834
HinfI GANTC 6 cut(s) 155, 244, 815, 863, 875, 1187
HpaII CCGG 1 cut(s) 452
HphI GGTGA 3 cut(s) 269, 814, 1111
Hpy166II GTNNAC 3 cut(s) 511, 834, 913
Hpy188I TCNGA 7 cut(s) 85, 358, 624, 655, 964, 978, 1192
Hpy188III TCNNGA 4 cut(s) 17, 391, 1131, 1202
Hpy8I GTNNAC 3 cut(s) 511, 834, 913
Hpy99I CGWCG 3 cut(s) 825, 908, 1092
HpyAV CCTTC 5 cut(s) 334, 617, 729, 959, 1155
HpyCH4III ACNGT 2 cut(s) 31, 754
HpyCH4IV ACGT 6 cut(s) 182, 318, 744, 1026, 1090, 1103
HpyCH4V TGCA 2 cut(s) 920, 1118
HpyF10VI GCNNNNNNNGC 4 cut(s) 435, 457, 780, 966
HpyF3I CTNAG 3 cut(s) 728, 827, 977
HpySE526I ACGT 6 cut(s) 182, 318, 744, 1026, 1090, 1103
Hsp92I GRCGYC 2 cut(s) 1026, 1103
Hsp92II CATG 2 cut(s) 446, 1045
Kzo9I GATC 3 cut(s) 164, 387, 1198
LmnI GCTCC 1 cut(s) 475
Lsp1109I GCAGC 3 cut(s) 447, 744, 747
MaeI CTAG 7 cut(s) 192, 209, 213, 809, 1136, 1164, 1202
MaeII ACGT 6 cut(s) 182, 318, 744, 1026, 1090, 1103
MaeIII GTNAC 5 cut(s) 275, 854, 1099, 1211, 1220
MalI GATC 3 cut(s) 166, 389, 1200
MboI GATC 3 cut(s) 164, 387, 1198
MboII GAAGA 8 cut(s) 124, 397, 581, 824, 993, 1022, 1094, 1208
MfeI CAATTG 1 cut(s) 90
MflI RGATCY 1 cut(s) 1198
MhlI GDGCHC 1 cut(s) 238
MluCI AATT 6 cut(s) 90, 515, 637, 992, 1108, 1243
MluI ACGCGT 1 cut(s) 570
MlyI GAGTC 4 cut(s) 238, 809, 884, 1181
MseI TTAA 2 cut(s) 618, 1249
MspA1I CMGCKG 1 cut(s) 438
MspI CCGG 1 cut(s) 452
MspR9I CCNGG 3 cut(s) 292, 453, 1226
MunI CAATTG 1 cut(s) 90
Mva1269I GAATGC 1 cut(s) 453
MvaI CCWGG 2 cut(s) 292, 1226
MvnI CGCG 1 cut(s) 572
MwoI GCNNNNNNNGC 4 cut(s) 435, 457, 780, 966
NciI CCSGG 1 cut(s) 453
NcoI CCATGG 1 cut(s) 1041
NdeII GATC 3 cut(s) 164, 387, 1198
NheI GCTAGC 3 cut(s) 191, 212, 1163
NlaIII CATG 2 cut(s) 446, 1045
NlaIV GGNNCC 3 cut(s) 42, 102, 235
NmuCI GTSAC 5 cut(s) 275, 854, 1099, 1211, 1220
PceI AGGCCT 1 cut(s) 795
PctI GAATGC 1 cut(s) 453
PfeI GAWTC 2 cut(s) 155, 863
PflMI CCANNNNNTGG 1 cut(s) 1047
PkrI GCNGC 4 cut(s) 325, 437, 734, 737
PleI GAGTC 4 cut(s) 238, 809, 883, 1181
PpsI GAGTC 4 cut(s) 238, 809, 883, 1181
Ppu21I YACGTR 1 cut(s) 319
PpuMI RGGWCCY 1 cut(s) 100
PshAI GACNNNNGTC 1 cut(s) 819
Psp5II RGGWCCY 1 cut(s) 100
Psp6I CCWGG 2 cut(s) 290, 1224
PspEI GGTNACC 1 cut(s) 275
PspGI CCWGG 2 cut(s) 290, 1224
PspN4I GGNNCC 3 cut(s) 42, 102, 235
PspPI GGNCC 3 cut(s) 100, 490, 599
PspPPI RGGWCCY 1 cut(s) 100
PsuI RGATCY 1 cut(s) 1198
RsaI GTAC 4 cut(s) 317, 582, 934, 1176
RsaNI GTAC 4 cut(s) 316, 581, 933, 1175
SaqAI TTAA 2 cut(s) 618, 1249
SatI GCNGC 4 cut(s) 324, 436, 733, 736
Sau3AI GATC 3 cut(s) 164, 387, 1198
Sau96I GGNCC 3 cut(s) 100, 490, 599
ScaI AGTACT 1 cut(s) 934
SchI GAGTC 4 cut(s) 238, 809, 884, 1181
ScrFI CCNGG 3 cut(s) 292, 453, 1226
SduI GDGCHC 1 cut(s) 238
SinI GGWCC 3 cut(s) 100, 490, 599
SnaBI TACGTA 1 cut(s) 319
SpeI ACTAGT 1 cut(s) 1135
Sse9I AATT 6 cut(s) 90, 515, 637, 992, 1108, 1243
SseBI AGGCCT 1 cut(s) 795
SsiI CCGC 7 cut(s) 323, 326, 333, 429, 438, 584, 665
SspMI CTAG 7 cut(s) 192, 209, 213, 809, 1136, 1164, 1202
StuI AGGCCT 1 cut(s) 795
StyD4I CCNGG 3 cut(s) 290, 451, 1224
StyI CCWWGG 4 cut(s) 65, 554, 595, 1041
TaaI ACNGT 2 cut(s) 31, 754
TaiI ACGT 6 cut(s) 185, 321, 747, 1029, 1093, 1106
TaqI TCGA 5 cut(s) 149, 416, 577, 903, 1239
TasI AATT 6 cut(s) 90, 515, 637, 992, 1108, 1243
TatI WGTACW 1 cut(s) 932
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 2 cut(s) 155, 863
Tru1I TTAA 2 cut(s) 618, 1249
Tru9I TTAA 2 cut(s) 618, 1249
TscAI CASTG 1 cut(s) 759
TseFI GTSAC 5 cut(s) 275, 854, 1099, 1211, 1220
TseI GCWGC 3 cut(s) 435, 732, 735
Tsp45I GTSAC 5 cut(s) 275, 854, 1099, 1211, 1220
TspDTI ATGAA 4 cut(s) 155, 685, 1085, 1209
TspRI CASTG 1 cut(s) 759
Van91I CCANNNNNTGG 1 cut(s) 1047
VpaK11BI GGWCC 3 cut(s) 100, 490, 599
XapI RAATTY 1 cut(s) 637
XbaI TCTAGA 1 cut(s) 1201
XspI CTAG 7 cut(s) 192, 209, 213, 809, 1136, 1164, 1202
ZraI GACGTC 2 cut(s) 1027, 1104
ZrmI AGTACT 1 cut(s) 934
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.