RLG00000027164

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0018043
Physical Location & Seq
Forward (+)
9198720 .. 9201311
2592 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027164

Sequence Viewer

Length: 1302 bp
ATGGATCCATCGAGCAAGGAGAAGTATCGTCCCAATTTGGACACAGTTCTAGAGGTCCCTCACAAGATCACAGTTCTAAAGGTCCCTCGCAAGAACACAGCAAATTTATGGAACAGAATGAAATCATTGATGAACCCGAGGGCACTGTCTCCCGATGTCATTAATGAACCCGATGGCAGGGCACTAGCTAGCTTACTTAGAGTTATTGGAGCTCCACTTGACCGCAACCTCATCCTAGGGCAAGGGGATGCCGGGGCTGAATACATTATGAAGCAATACGTGGCGGCGTTGGGCGGGGACAAGGCTTTGTCATCAATGGACAGCTTGTGTGCGACGGGCAAGGTGCAGATCACAGTGTCAAATGGGACGAAAGGAAGCTTGAAATCCATTGGCGGTGGGGGAGGAATCGGAGGACATGGAATGGGAGGGTTTGTGCTCTGGCAAAAGAGGGAGCTATGGTGCTTGGAAGTGATGTTATCTACTGGTTACAAAGTTAGCACGGGGAGTGATGGCAAGGCGCCTTTGCCTTGGCATCACTCGTACGCTTATCGAGTCCCGCCTCTTCCCCTCGGACATCTCCTTCAGGCATATATCATGGATTCGGATCCTCTAAAGTTTACTTTAATTGGTCTTAATCCAAGGTCAACAGCAAATTTATTCAGAACCTCCGTTTGTTTTGCGGACAAACCAATAAACAAAGAGGACTGCTTCAAACTAAAACTGAAGGTTAAACCTTCAACACTAAGCAGCAGCAACGTAGAGATAGTTCGACACACCGTTTTGGGCTACTTCAGCCACAAAACAGGTCTTTTGGTGAAGCTAGAAGACTCTCGCCTACTAAGGATGACAGCTTTCGGAAAGCACAGTTATTGGGAATCGACAATGGAGTCAGACTTCCAAGACTACAGAACCATCGACGGCCTCAACATCGCACACGGGGGTAGGACTCGTGTGTCTTCGTGTGGTGAGAGCTCGGAAGGCCATCCCCCGAGAAGAATAGTGAAATTGGTTTGGGCACTCGAAGAAGTAGGTTTTGACATTAAGGGGTTGTCCGAGGAGTGCTTCTTGCCTCCTGGTGACTTAAAGAAAGAGGAAGAAAGCTGCGGTATGGTGAAATCTAAATCTTGTGCAAGATTAACATCCAGATCAATTCCATCTCCTTTGAGATTTCAAGCTACCAAAGTAGTTCCCAGTGATGATTCTAATCATGATCTAGAAGGTAGCGACATTGGTGACCAGGAGGATCTTCAAGATTTCGAGAGCTGCAACAGTAGTGACCATGATGATTCTGATCAATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

434

Amino Acids

47.5

Weight (kDa)

7.17

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 110 - 358 4e-65 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018043)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g14750
rosa_chinensis RchiOBHm_Chr1g0369021
rosa_laevigata RLG00000027164
rosa_multiflora Rmu_sc0004250.1_g000030
rosa_roxburghii Rroxscaffold_4G00288080
rosa_rugosa Rorug01G0347000
rosa_samantha Rh1AG353900 Rh1BG317400 Rh1CG331900 Rh1DG348300
rosa_wichuraiana Rw1G031270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 886, 952
AccB1I GGYRCC 1 cut(s) 517
AciI CCGC 7 cut(s) 223, 284, 294, 393, 557, 680, 1104
AclWI GGATC 4 cut(s) 12, 599, 612, 1251
AcsI RAATTY 2 cut(s) 103, 652
AcuI CTGAAG 3 cut(s) 566, 743, 775
AcyI GRCGYC 1 cut(s) 518
AfaI GTAC 1 cut(s) 542
AfiI CCNNNNNNNGG 1 cut(s) 177
AgsI TTSAA 5 cut(s) 382, 712, 738, 1172, 1250
AjnI CCWGG 2 cut(s) 1072, 1236
Alw21I GWGCWC 3 cut(s) 214, 438, 974
Alw26I GTCTC 1 cut(s) 153
AlwI GGATC 4 cut(s) 12, 599, 612, 1251
Ama87I CYCGRG 2 cut(s) 136, 988
AoxI GGCC 2 cut(s) 919, 979
ApeKI GCWGC 4 cut(s) 747, 750, 1101, 1263
ApoI RAATTY 2 cut(s) 103, 652
AseI ATTAAT 1 cut(s) 162
AspA2I CCTAGG 1 cut(s) 235
AspLEI GCGC 1 cut(s) 520
AspS9I GGNCC 2 cut(s) 55, 82
AsuC2I CCSGG 1 cut(s) 253
AsuHPI GGTGA 5 cut(s) 826, 977, 1088, 1123, 1244
AsuNHI GCTAGC 1 cut(s) 188
AvaI CYCGRG 2 cut(s) 136, 988
AvaII GGWCC 2 cut(s) 55, 82
AvrII CCTAGG 1 cut(s) 235
BaeGI GKGCMC 3 cut(s) 145, 184, 1018
BamHI GGATCC 2 cut(s) 4, 604
BanI GGYRCC 1 cut(s) 517
BanII GRGCYC 2 cut(s) 214, 974
BauI CACGAG 1 cut(s) 948
BbsI GAAGAC 2 cut(s) 831, 948
Bbv12I GWGCWC 3 cut(s) 214, 438, 974
BbvI GCAGC 4 cut(s) 759, 762, 1088, 1250
BccI CCATC 6 cut(s) 16, 167, 503, 920, 990, 1162
BceAI ACGGC 1 cut(s) 934
BciT130I CCWGG 2 cut(s) 1074, 1238
BclI TGATCA 1 cut(s) 1291
BcnI CCSGG 1 cut(s) 253
BcoDI GTCTC 1 cut(s) 153
BfaI CTAG 6 cut(s) 50, 185, 189, 236, 821, 1214
BfmI CTRYAG 1 cut(s) 904
BfoI RGCGCY 1 cut(s) 521
BisI GCNGC 5 cut(s) 285, 748, 751, 1102, 1264
BlnI CCTAGG 1 cut(s) 235
BlsI GCNGC 5 cut(s) 286, 749, 752, 1103, 1265
Bme1390I CCNGG 3 cut(s) 253, 1074, 1238
Bme18I GGWCC 2 cut(s) 55, 82
BmeT110I CYCGRG 2 cut(s) 136, 988
BmgT120I GGNCC 2 cut(s) 55, 82
BmiI GGNNCC 5 cut(s) 6, 57, 84, 519, 606
BmrFI CCNGG 3 cut(s) 253, 1074, 1238
BmrI ACTGGG 1 cut(s) 1185
BmsI GCATC 2 cut(s) 238, 541
BmtI GCTAGC 1 cut(s) 192
BmuI ACTGGG 1 cut(s) 1185
BpiI GAAGAC 2 cut(s) 831, 948
BpuMI CCSGG 1 cut(s) 253
BsaAI YACGTR 1 cut(s) 280
BsaBI GATNNNNATC 4 cut(s) 603, 1138, 1203, 1290
BsaHI GRCGYC 1 cut(s) 518
BsaJI CCNNGG 7 cut(s) 137, 235, 252, 527, 568, 638, 1053
BsaXI ACNNNNNCTCC 4 cut(s) 417, 443, 447, 473
Bsc4I CCNNNNNNNGG 1 cut(s) 177
Bse1I ACTGG 2 cut(s) 487, 1191
Bse8I GATNNNNATC 4 cut(s) 603, 1138, 1203, 1290
BseBI CCWGG 2 cut(s) 1074, 1238
BseDI CCNNGG 7 cut(s) 137, 235, 252, 527, 568, 638, 1053
BseGI GGATG 5 cut(s) 231, 253, 849, 982, 1139
BseJI GATNNNNATC 4 cut(s) 603, 1138, 1203, 1290
BseLI CCNNNNNNNGG 1 cut(s) 177
BseNI ACTGG 2 cut(s) 487, 1191
BseRI GAGGAG 1 cut(s) 1070
BseSI GKGCMC 3 cut(s) 145, 184, 1018
BseXI GCAGC 4 cut(s) 759, 762, 1088, 1250
BsgI GTGCAG 1 cut(s) 365
BshFI GGCC 2 cut(s) 921, 981
BshNI GGYRCC 1 cut(s) 517
BsiHKAI GWGCWC 3 cut(s) 214, 438, 974
BsiHKCI CYCGRG 2 cut(s) 136, 988
BsiSI CCGG 1 cut(s) 252
BsiWI CGTACG 1 cut(s) 540
BslFI GGGAC 6 cut(s) 15, 41, 68, 311, 379, 539
BslI CCNNNNNNNGG 1 cut(s) 177
BsmAI GTCTC 1 cut(s) 153
BsmFI GGGAC 6 cut(s) 15, 41, 68, 311, 379, 539
BsnI GGCC 2 cut(s) 921, 981
BsoBI CYCGRG 2 cut(s) 136, 988
Bsp1286I GDGCHC 6 cut(s) 145, 184, 214, 438, 974, 1018
Bsp143I GATC 8 cut(s) 4, 66, 348, 604, 1145, 1210, 1243, 1291
BspACI CCGC 7 cut(s) 223, 284, 294, 393, 557, 680, 1104
BspANI GGCC 2 cut(s) 921, 981
BspHI TCATGA 1 cut(s) 1207
BspLI GGNNCC 5 cut(s) 6, 57, 84, 519, 606
BspOI GCTAGC 1 cut(s) 192
BspPI GGATC 4 cut(s) 12, 599, 612, 1251
BspT107I GGYRCC 1 cut(s) 517
BsrI ACTGG 2 cut(s) 487, 1191
BssECI CCNNGG 7 cut(s) 137, 235, 252, 527, 568, 638, 1053
BssMI GATC 8 cut(s) 4, 66, 348, 604, 1145, 1210, 1243, 1291
BssNI GRCGYC 1 cut(s) 518
BssSI CACGAG 1 cut(s) 948
BssT1I CCWWGG 3 cut(s) 235, 527, 638
Bst2BI CACGAG 1 cut(s) 948
Bst2UI CCWGG 2 cut(s) 1074, 1238
Bst4CI ACNGT 7 cut(s) 46, 73, 147, 355, 778, 866, 1271
Bst6I CTCTTC 1 cut(s) 567
BstACI GRCGYC 1 cut(s) 518
BstBAI YACGTR 1 cut(s) 280
BstC8I GCNNGC 1 cut(s) 190
BstDEI CTNAG 3 cut(s) 197, 743, 839
BstEII GGTNACC 1 cut(s) 1232
BstF5I GGATG 5 cut(s) 231, 253, 849, 982, 1139
BstH2I RGCGCY 1 cut(s) 521
BstHHI GCGC 1 cut(s) 520
BstKTI GATC 8 cut(s) 7, 69, 351, 607, 1148, 1213, 1246, 1294
BstMAI GTCTC 1 cut(s) 153
BstMBI GATC 8 cut(s) 4, 66, 348, 604, 1145, 1210, 1243, 1291
BstMWI GCNNNNNNNGC 2 cut(s) 792, 978
BstNI CCWGG 2 cut(s) 1074, 1238
BstPI GGTNACC 1 cut(s) 1232
BstSCI CCNGG 3 cut(s) 251, 1072, 1236
BstSFI CTRYAG 1 cut(s) 904
BstSLI GKGCMC 3 cut(s) 145, 184, 1018
BstV1I GCAGC 4 cut(s) 759, 762, 1088, 1250
BstV2I GAAGAC 2 cut(s) 831, 948
BstX2I RGATCY 3 cut(s) 4, 604, 1243
BstYI RGATCY 3 cut(s) 4, 604, 1243
BsuRI GGCC 2 cut(s) 921, 981
BtgZI GCGATG 1 cut(s) 913
BtsCI GGATG 5 cut(s) 231, 253, 849, 982, 1139
BtsIMutI CAGTG 3 cut(s) 143, 360, 1198
Cac8I GCNNGC 1 cut(s) 190
CciI TCATGA 1 cut(s) 1207
CfoI GCGC 1 cut(s) 520
Cfr13I GGNCC 2 cut(s) 55, 82
Csp6I GTAC 1 cut(s) 541
CviAII CATG 4 cut(s) 416, 595, 1208, 1280
CviQI GTAC 1 cut(s) 541
DdeI CTNAG 3 cut(s) 197, 743, 839
DinI GGCGCC 1 cut(s) 519
DpnI GATC 8 cut(s) 6, 68, 350, 606, 1147, 1212, 1245, 1293
DpnII GATC 8 cut(s) 4, 66, 348, 604, 1145, 1210, 1243, 1291
DrdI GACNNNNNNGTC 2 cut(s) 886, 952
DseDI GACNNNNNNGTC 2 cut(s) 886, 952
Eam1104I CTCTTC 1 cut(s) 567
EarI CTCTTC 1 cut(s) 567
Ecl136II GAGCTC 2 cut(s) 212, 972
Eco130I CCWWGG 3 cut(s) 235, 527, 638
Eco24I GRGCYC 2 cut(s) 214, 974
Eco47I GGWCC 2 cut(s) 55, 82
Eco53kI GAGCTC 2 cut(s) 212, 972
Eco57I CTGAAG 3 cut(s) 566, 743, 775
Eco88I CYCGRG 2 cut(s) 136, 988
Eco91I GGTNACC 1 cut(s) 1232
EcoICRI GAGCTC 2 cut(s) 212, 972
EcoO109I RGGNCCY 2 cut(s) 55, 82
EcoO65I GGTNACC 1 cut(s) 1232
EcoRII CCWGG 2 cut(s) 1072, 1236
EcoT14I CCWWGG 3 cut(s) 235, 527, 638
EcoT38I GRGCYC 2 cut(s) 214, 974
EgeI GGCGCC 1 cut(s) 519
EheI GGCGCC 1 cut(s) 519
ErhI CCWWGG 3 cut(s) 235, 527, 638
FaeI CATG 4 cut(s) 419, 598, 1211, 1283
FaqI GGGAC 6 cut(s) 15, 41, 68, 311, 379, 539
FatI CATG 4 cut(s) 415, 594, 1207, 1279
FauI CCCGC 2 cut(s) 287, 564
FbaI TGATCA 1 cut(s) 1291
Fnu4HI GCNGC 5 cut(s) 285, 748, 751, 1102, 1264
FokI GGATG 5 cut(s) 218, 260, 856, 969, 1126
FriOI GRGCYC 2 cut(s) 214, 974
Fsp4HI GCNGC 5 cut(s) 285, 748, 751, 1102, 1264
FspBI CTAG 6 cut(s) 50, 185, 189, 236, 821, 1214
GlaI GCGC 1 cut(s) 519
GluI GCNGC 5 cut(s) 285, 748, 751, 1102, 1264
HaeII RGCGCY 1 cut(s) 521
HaeIII GGCC 2 cut(s) 921, 981
HapII CCGG 1 cut(s) 252
HhaI GCGC 1 cut(s) 520
Hin1I GRCGYC 1 cut(s) 518
Hin1II CATG 4 cut(s) 419, 598, 1211, 1283
Hin6I GCGC 1 cut(s) 518
HinP1I GCGC 1 cut(s) 518
HincII GTYRAC 1 cut(s) 645
HindII GTYRAC 1 cut(s) 645
HindIII AAGCTT 1 cut(s) 376
HinfI GANTC 9 cut(s) 405, 552, 599, 827, 875, 887, 946, 1199, 1286
HpaII CCGG 1 cut(s) 252
HphI GGTGA 5 cut(s) 826, 977, 1088, 1123, 1244
Hpy166II GTNNAC 2 cut(s) 618, 645
Hpy188I TCNGA 9 cut(s) 410, 572, 604, 662, 857, 892, 976, 1054, 1291
Hpy188III TCNNGA 7 cut(s) 50, 152, 1143, 1208, 1214, 1250, 1258
Hpy8I GTNNAC 2 cut(s) 618, 645
Hpy99I CGWCG 2 cut(s) 337, 920
HpyAV CCTTC 5 cut(s) 590, 718, 744, 971, 1211
HpyCH4III ACNGT 7 cut(s) 46, 73, 147, 355, 778, 866, 1271
HpyCH4IV ACGT 2 cut(s) 279, 756
HpyCH4V TGCA 3 cut(s) 346, 1130, 1266
HpyF10VI GCNNNNNNNGC 2 cut(s) 792, 978
HpyF3I CTNAG 3 cut(s) 197, 743, 839
HpySE526I ACGT 2 cut(s) 279, 756
Hsp92I GRCGYC 1 cut(s) 518
Hsp92II CATG 4 cut(s) 419, 598, 1211, 1283
HspAI GCGC 1 cut(s) 518
KasI GGCGCC 1 cut(s) 517
Ksp22I TGATCA 1 cut(s) 1291
Kzo9I GATC 8 cut(s) 4, 66, 348, 604, 1145, 1210, 1243, 1291
LmnI GCTCC 3 cut(s) 209, 217, 451
Lsp1109I GCAGC 4 cut(s) 759, 762, 1088, 1250
LweI GCATC 2 cut(s) 238, 541
MaeI CTAG 6 cut(s) 50, 185, 189, 236, 821, 1214
MaeII ACGT 2 cut(s) 279, 756
MaeIII GTNAC 4 cut(s) 485, 1076, 1232, 1274
MalI GATC 8 cut(s) 6, 68, 350, 606, 1147, 1212, 1245, 1293
MboI GATC 8 cut(s) 4, 66, 348, 604, 1145, 1210, 1243, 1291
MboII GAAGA 7 cut(s) 554, 836, 948, 1005, 1034, 1106, 1238
MflI RGATCY 3 cut(s) 4, 604, 1243
MhlI GDGCHC 6 cut(s) 145, 184, 214, 438, 974, 1018
MluCI AATT 6 cut(s) 34, 103, 624, 652, 1004, 1149
Mly113I GGCGCC 1 cut(s) 518
MlyI GAGTC 4 cut(s) 561, 821, 896, 940
MseI TTAA 8 cut(s) 162, 623, 633, 729, 1041, 1082, 1136, 1300
MspI CCGG 1 cut(s) 252
MspR9I CCNGG 3 cut(s) 253, 1074, 1238
MvaI CCWGG 2 cut(s) 1074, 1238
MwoI GCNNNNNNNGC 2 cut(s) 792, 978
NarI GGCGCC 1 cut(s) 518
NciI CCSGG 1 cut(s) 253
NdeII GATC 8 cut(s) 4, 66, 348, 604, 1145, 1210, 1243, 1291
NheI GCTAGC 1 cut(s) 188
NlaIII CATG 4 cut(s) 419, 598, 1211, 1283
NlaIV GGNNCC 5 cut(s) 6, 57, 84, 519, 606
NmuCI GTSAC 3 cut(s) 1076, 1232, 1274
PagI TCATGA 1 cut(s) 1207
PfeI GAWTC 5 cut(s) 405, 599, 875, 1199, 1286
Pfl23II CGTACG 1 cut(s) 540
PkrI GCNGC 5 cut(s) 286, 749, 752, 1103, 1265
PleI GAGTC 4 cut(s) 560, 821, 895, 940
PluTI GGCGCC 1 cut(s) 521
PpsI GAGTC 4 cut(s) 560, 821, 895, 940
Ppu21I YACGTR 1 cut(s) 280
PpuMI RGGWCCY 2 cut(s) 55, 82
PshBI ATTAAT 1 cut(s) 162
Psp124BI GAGCTC 2 cut(s) 214, 974
Psp5II RGGWCCY 2 cut(s) 55, 82
Psp6I CCWGG 2 cut(s) 1072, 1236
PspEI GGTNACC 1 cut(s) 1232
PspGI CCWGG 2 cut(s) 1072, 1236
PspLI CGTACG 1 cut(s) 540
PspN4I GGNNCC 5 cut(s) 6, 57, 84, 519, 606
PspPI GGNCC 2 cut(s) 55, 82
PspPPI RGGWCCY 2 cut(s) 55, 82
PsrI GAACNNNNNNTAC 2 cut(s) 750, 782
PsuI RGATCY 3 cut(s) 4, 604, 1243
RsaI GTAC 1 cut(s) 542
RsaNI GTAC 1 cut(s) 541
SacI GAGCTC 2 cut(s) 214, 974
SaqAI TTAA 8 cut(s) 162, 623, 633, 729, 1041, 1082, 1136, 1300
SatI GCNGC 5 cut(s) 285, 748, 751, 1102, 1264
Sau3AI GATC 8 cut(s) 4, 66, 348, 604, 1145, 1210, 1243, 1291
Sau96I GGNCC 2 cut(s) 55, 82
SchI GAGTC 4 cut(s) 561, 821, 896, 940
ScrFI CCNGG 3 cut(s) 253, 1074, 1238
SduI GDGCHC 6 cut(s) 145, 184, 214, 438, 974, 1018
SfaNI GCATC 2 cut(s) 238, 541
SfcI CTRYAG 1 cut(s) 904
SfoI GGCGCC 1 cut(s) 519
SinI GGWCC 2 cut(s) 55, 82
Sse9I AATT 6 cut(s) 34, 103, 624, 652, 1004, 1149
SsiI CCGC 7 cut(s) 223, 284, 294, 393, 557, 680, 1104
SspDI GGCGCC 1 cut(s) 517
SspMI CTAG 6 cut(s) 50, 185, 189, 236, 821, 1214
SstI GAGCTC 2 cut(s) 214, 974
StyD4I CCNGG 3 cut(s) 251, 1072, 1236
StyI CCWWGG 3 cut(s) 235, 527, 638
TaaI ACNGT 7 cut(s) 46, 73, 147, 355, 778, 866, 1271
TaiI ACGT 2 cut(s) 282, 759
TaqI TCGA 7 cut(s) 11, 550, 769, 878, 915, 1020, 1257
TasI AATT 6 cut(s) 34, 103, 624, 652, 1004, 1149
TauI GCSGC 1 cut(s) 287
TfiI GAWTC 5 cut(s) 405, 599, 875, 1199, 1286
Tru1I TTAA 8 cut(s) 162, 623, 633, 729, 1041, 1082, 1136, 1300
Tru9I TTAA 8 cut(s) 162, 623, 633, 729, 1041, 1082, 1136, 1300
TscAI CASTG 3 cut(s) 150, 360, 1198
TseFI GTSAC 3 cut(s) 1076, 1232, 1274
TseI GCWGC 4 cut(s) 747, 750, 1101, 1263
Tsp45I GTSAC 3 cut(s) 1076, 1232, 1274
TspDTI ATGAA 4 cut(s) 134, 146, 180, 284
TspGWI ACGGA 1 cut(s) 658
TspRI CASTG 3 cut(s) 150, 360, 1198
VpaK11BI GGWCC 2 cut(s) 55, 82
VspI ATTAAT 1 cut(s) 162
XapI RAATTY 2 cut(s) 103, 652
XbaI TCTAGA 2 cut(s) 49, 1213
XmaJI CCTAGG 1 cut(s) 235
XspI CTAG 6 cut(s) 50, 185, 189, 236, 821, 1214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.