Rh1AG353900

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0018043
Physical Location & Seq
Reverse (-)
58991457 .. 58992642
1186 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG353900.1

Sequence Viewer

Length: 837 bp
ATGCCAGGGTTTGTGCTCTGGCAAAAGAGGGAGGTATGGTGCTTGGAAGTGATGTTGTCTACTGGTTACAAAGTCAGCACGGGGAGTGATGGCAAGGCGCCTTTGCCTTGGCATCACTCGTACGCTTATCGAGTCCCACCTCTTCCCCTCGGACATCTCCTTCAGGGTCTTAATCCAAGGTCAACAGCAAATTTATTCAGAAACTCCTTTTGTTCTGCGGACAAACCAATAAACAAAGAGGACTGCTTCATACTAAAACTGAAGGTTAAACCTTCAACACTAAGCAGCAGCAACGTAGAGATAGTTCGACAAACCGTTTTGGGCTACTTCAGCCACAAAACAGGCCTTTTGGTGAAGCTAGAAGACTCTCGCCAACTAAGGACGACAGCTTTCGGAAAGCACAGTTATTGGGAATCGACAATGGAGTCAGACTTCCAAGACTACAGAACCATCGACGGCCTCAACATCGCACACGGGGGTAGGACTTGTGTGTCTTTGTGTGGTGAGAGCTCGGAAGGCCATCCCCGGAGAAGAATAGTGAAATTGGTTTGGGCACTCGAAGAAGTAGGTTTTGACATTAAGGGGTTGTCCGTGGAGTGCTTCTTGCCTCCTGGTGACTTAAAGAAAGAGGAAGAAAGCTGCGGCATGGTGAAATCTAAATCTTGTGCAAAATTAACATCCAGAATTCCATCTCCTTTGAGATTTCAAGCTACCAAAGTAGTTCCCAGTGATGATTCTAATCATGATCTAGAAGGTAGCGACATTAGTGACCAGGAGGATCTTCAAGATTTCGAGAGCTGCAACAGTAGTGACCTTGATGATTCTGATCAATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

278

Amino Acids

31.06

Weight (kDa)

5.93

Isoelectric Point (pI)

53.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 3 - 204 3.7e-66 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018043)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g14750
rosa_chinensis RchiOBHm_Chr1g0369021
rosa_laevigata RLG00000027164
rosa_multiflora Rmu_sc0004250.1_g000030
rosa_roxburghii Rroxscaffold_4G00288080
rosa_rugosa Rorug01G0347000
rosa_samantha Rh1AG353900 Rh1BG317400 Rh1CG331900 Rh1DG348300
rosa_wichuraiana Rw1G031270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 424, 490
AccB1I GGYRCC 1 cut(s) 97
AccI GTMKAC 1 cut(s) 59
AciI CCGC 2 cut(s) 218, 642
AclWI GGATC 1 cut(s) 786
AcsI RAATTY 2 cut(s) 190, 684
AcuI CTGAAG 3 cut(s) 146, 281, 313
AcyI GRCGYC 1 cut(s) 98
AfaI GTAC 1 cut(s) 122
AgsI TTSAA 3 cut(s) 276, 707, 785
AjnI CCWGG 3 cut(s) 4, 610, 771
AluBI AGCT 6 cut(s) 358, 389, 510, 639, 710, 798
AluI AGCT 6 cut(s) 358, 389, 510, 639, 710, 798
Alw21I GWGCWC 2 cut(s) 18, 512
AlwI GGATC 1 cut(s) 786
AoxI GGCC 3 cut(s) 343, 457, 517
ApeKI GCWGC 4 cut(s) 285, 288, 639, 798
ApoI RAATTY 2 cut(s) 190, 684
AspLEI GCGC 1 cut(s) 100
AsuC2I CCSGG 1 cut(s) 526
AsuHPI GGTGA 4 cut(s) 364, 515, 626, 661
BaeGI GKGCMC 1 cut(s) 556
BanI GGYRCC 1 cut(s) 97
BanII GRGCYC 1 cut(s) 512
BbsI GAAGAC 1 cut(s) 369
Bbv12I GWGCWC 2 cut(s) 18, 512
BbvI GCAGC 4 cut(s) 297, 300, 626, 785
BccI CCATC 4 cut(s) 83, 458, 528, 697
BceAI ACGGC 1 cut(s) 472
BciT130I CCWGG 3 cut(s) 6, 612, 773
BclI TGATCA 1 cut(s) 826
BcnI CCSGG 1 cut(s) 526
BfaI CTAG 2 cut(s) 359, 749
BfmI CTRYAG 1 cut(s) 442
BfoI RGCGCY 1 cut(s) 101
BisI GCNGC 5 cut(s) 286, 289, 640, 643, 799
BlsI GCNGC 5 cut(s) 287, 290, 641, 644, 800
Bme1390I CCNGG 4 cut(s) 6, 526, 612, 773
BmiI GGNNCC 1 cut(s) 99
BmrFI CCNGG 4 cut(s) 6, 526, 612, 773
BmrI ACTGGG 1 cut(s) 720
BmsI GCATC 1 cut(s) 121
BmuI ACTGGG 1 cut(s) 720
BpiI GAAGAC 1 cut(s) 369
BpuMI CCSGG 1 cut(s) 526
BsaBI GATNNNNATC 2 cut(s) 738, 825
BsaHI GRCGYC 1 cut(s) 98
BsaJI CCNNGG 6 cut(s) 5, 107, 148, 176, 524, 591
BsaXI ACNNNNNCTCC 2 cut(s) 23, 53
Bse1I ACTGG 2 cut(s) 67, 726
Bse8I GATNNNNATC 2 cut(s) 738, 825
BseBI CCWGG 3 cut(s) 6, 612, 773
BseDI CCNNGG 6 cut(s) 5, 107, 148, 176, 524, 591
BseGI GGATG 2 cut(s) 520, 677
BseJI GATNNNNATC 2 cut(s) 738, 825
BseNI ACTGG 2 cut(s) 67, 726
BseSI GKGCMC 1 cut(s) 556
BseXI GCAGC 4 cut(s) 297, 300, 626, 785
BshFI GGCC 3 cut(s) 345, 459, 519
BshNI GGYRCC 1 cut(s) 97
BsiHKAI GWGCWC 2 cut(s) 18, 512
BsiSI CCGG 1 cut(s) 526
BsiWI CGTACG 1 cut(s) 120
BslFI GGGAC 1 cut(s) 119
BsmFI GGGAC 1 cut(s) 119
BsnI GGCC 3 cut(s) 345, 459, 519
Bsp1286I GDGCHC 3 cut(s) 18, 512, 556
Bsp143I GATC 3 cut(s) 745, 778, 826
BspACI CCGC 2 cut(s) 218, 642
BspANI GGCC 3 cut(s) 345, 459, 519
BspHI TCATGA 1 cut(s) 742
BspLI GGNNCC 1 cut(s) 99
BspPI GGATC 1 cut(s) 786
BspT107I GGYRCC 1 cut(s) 97
BsrI ACTGG 2 cut(s) 67, 726
BssECI CCNNGG 6 cut(s) 5, 107, 148, 176, 524, 591
BssMI GATC 3 cut(s) 745, 778, 826
BssNI GRCGYC 1 cut(s) 98
BssT1I CCWWGG 2 cut(s) 107, 176
Bst2UI CCWGG 3 cut(s) 6, 612, 773
Bst4CI ACNGT 3 cut(s) 316, 404, 806
Bst6I CTCTTC 1 cut(s) 147
BstACI GRCGYC 1 cut(s) 98
BstDEI CTNAG 2 cut(s) 281, 377
BstDSI CCRYGG 1 cut(s) 591
BstF5I GGATG 2 cut(s) 520, 677
BstH2I RGCGCY 1 cut(s) 101
BstHHI GCGC 1 cut(s) 100
BstKTI GATC 3 cut(s) 748, 781, 829
BstMBI GATC 3 cut(s) 745, 778, 826
BstMWI GCNNNNNNNGC 2 cut(s) 330, 516
BstNI CCWGG 3 cut(s) 6, 612, 773
BstSCI CCNGG 4 cut(s) 4, 524, 610, 771
BstSFI CTRYAG 1 cut(s) 442
BstSLI GKGCMC 1 cut(s) 556
BstV1I GCAGC 4 cut(s) 297, 300, 626, 785
BstV2I GAAGAC 1 cut(s) 369
BstX2I RGATCY 1 cut(s) 778
BstYI RGATCY 1 cut(s) 778
BsuRI GGCC 3 cut(s) 345, 459, 519
BtgI CCRYGG 1 cut(s) 591
BtgZI GCGATG 1 cut(s) 451
BtsCI GGATG 2 cut(s) 520, 677
BtsIMutI CAGTG 1 cut(s) 733
CciI TCATGA 1 cut(s) 742
CfoI GCGC 1 cut(s) 100
Csp6I GTAC 1 cut(s) 121
CviAII CATG 2 cut(s) 646, 743
CviQI GTAC 1 cut(s) 121
DdeI CTNAG 2 cut(s) 281, 377
DinI GGCGCC 1 cut(s) 99
DpnI GATC 3 cut(s) 747, 780, 828
DpnII GATC 3 cut(s) 745, 778, 826
DrdI GACNNNNNNGTC 2 cut(s) 424, 490
DseDI GACNNNNNNGTC 2 cut(s) 424, 490
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
Ecl136II GAGCTC 1 cut(s) 510
Eco130I CCWWGG 2 cut(s) 107, 176
Eco147I AGGCCT 1 cut(s) 345
Eco24I GRGCYC 1 cut(s) 512
Eco53kI GAGCTC 1 cut(s) 510
Eco57I CTGAAG 3 cut(s) 146, 281, 313
EcoICRI GAGCTC 1 cut(s) 510
EcoRI GAATTC 1 cut(s) 684
EcoRII CCWGG 3 cut(s) 4, 610, 771
EcoT14I CCWWGG 2 cut(s) 107, 176
EcoT38I GRGCYC 1 cut(s) 512
EgeI GGCGCC 1 cut(s) 99
EheI GGCGCC 1 cut(s) 99
ErhI CCWWGG 2 cut(s) 107, 176
FaeI CATG 2 cut(s) 649, 746
FaiI YATR 4 cut(s) 37, 251, 647, 744
FaqI GGGAC 1 cut(s) 119
FatI CATG 2 cut(s) 645, 742
FbaI TGATCA 1 cut(s) 826
FblI GTMKAC 1 cut(s) 59
Fnu4HI GCNGC 5 cut(s) 286, 289, 640, 643, 799
FokI GGATG 2 cut(s) 507, 664
FriOI GRGCYC 1 cut(s) 512
Fsp4HI GCNGC 5 cut(s) 286, 289, 640, 643, 799
FspBI CTAG 2 cut(s) 359, 749
GlaI GCGC 1 cut(s) 99
GluI GCNGC 5 cut(s) 286, 289, 640, 643, 799
HaeII RGCGCY 1 cut(s) 101
HaeIII GGCC 3 cut(s) 345, 459, 519
HapII CCGG 1 cut(s) 526
HhaI GCGC 1 cut(s) 100
Hin1I GRCGYC 1 cut(s) 98
Hin1II CATG 2 cut(s) 649, 746
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HincII GTYRAC 1 cut(s) 183
HindII GTYRAC 1 cut(s) 183
HinfI GANTC 6 cut(s) 132, 365, 413, 425, 734, 821
HpaII CCGG 1 cut(s) 526
HphI GGTGA 4 cut(s) 364, 515, 626, 661
Hpy166II GTNNAC 2 cut(s) 60, 183
Hpy188I TCNGA 6 cut(s) 152, 200, 395, 430, 514, 826
Hpy188III TCNNGA 5 cut(s) 681, 743, 749, 785, 793
Hpy8I GTNNAC 2 cut(s) 60, 183
Hpy99I CGWCG 1 cut(s) 458
HpyAV CCTTC 5 cut(s) 170, 256, 282, 509, 746
HpyCH4III ACNGT 3 cut(s) 316, 404, 806
HpyCH4IV ACGT 1 cut(s) 294
HpyCH4V TGCA 2 cut(s) 668, 801
HpyF10VI GCNNNNNNNGC 2 cut(s) 330, 516
HpyF3I CTNAG 2 cut(s) 281, 377
HpySE526I ACGT 1 cut(s) 294
Hsp92I GRCGYC 1 cut(s) 98
Hsp92II CATG 2 cut(s) 649, 746
HspAI GCGC 1 cut(s) 98
KasI GGCGCC 1 cut(s) 97
Ksp22I TGATCA 1 cut(s) 826
Kzo9I GATC 3 cut(s) 745, 778, 826
Lsp1109I GCAGC 4 cut(s) 297, 300, 626, 785
LweI GCATC 1 cut(s) 121
MaeI CTAG 2 cut(s) 359, 749
MaeII ACGT 1 cut(s) 294
MaeIII GTNAC 4 cut(s) 65, 614, 767, 809
MalI GATC 3 cut(s) 747, 780, 828
MboI GATC 3 cut(s) 745, 778, 826
MboII GAAGA 6 cut(s) 134, 374, 543, 572, 644, 773
MflI RGATCY 1 cut(s) 778
MhlI GDGCHC 3 cut(s) 18, 512, 556
MluCI AATT 4 cut(s) 190, 542, 671, 684
Mly113I GGCGCC 1 cut(s) 98
MlyI GAGTC 3 cut(s) 141, 359, 434
MnlI CCTC 9 cut(s) 21, 25, 150, 158, 232, 470, 618, 622, 769
MseI TTAA 6 cut(s) 171, 267, 579, 620, 674, 835
MspI CCGG 1 cut(s) 526
MspR9I CCNGG 4 cut(s) 6, 526, 612, 773
MvaI CCWGG 3 cut(s) 6, 612, 773
MwoI GCNNNNNNNGC 2 cut(s) 330, 516
NarI GGCGCC 1 cut(s) 98
NciI CCSGG 1 cut(s) 526
NdeII GATC 3 cut(s) 745, 778, 826
NlaIII CATG 2 cut(s) 649, 746
NlaIV GGNNCC 1 cut(s) 99
NmuCI GTSAC 3 cut(s) 614, 767, 809
PagI TCATGA 1 cut(s) 742
PceI AGGCCT 1 cut(s) 345
PfeI GAWTC 3 cut(s) 413, 734, 821
Pfl23II CGTACG 1 cut(s) 120
PkrI GCNGC 5 cut(s) 287, 290, 641, 644, 800
PleI GAGTC 3 cut(s) 140, 359, 433
PluTI GGCGCC 1 cut(s) 101
PpsI GAGTC 3 cut(s) 140, 359, 433
Psp124BI GAGCTC 1 cut(s) 512
Psp6I CCWGG 3 cut(s) 4, 610, 771
PspGI CCWGG 3 cut(s) 4, 610, 771
PspLI CGTACG 1 cut(s) 120
PspN4I GGNNCC 1 cut(s) 99
PsrI GAACNNNNNNTAC 2 cut(s) 288, 320
PsuI RGATCY 1 cut(s) 778
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
SacI GAGCTC 1 cut(s) 512
SaqAI TTAA 6 cut(s) 171, 267, 579, 620, 674, 835
SatI GCNGC 5 cut(s) 286, 289, 640, 643, 799
Sau3AI GATC 3 cut(s) 745, 778, 826
SchI GAGTC 3 cut(s) 141, 359, 434
ScrFI CCNGG 4 cut(s) 6, 526, 612, 773
SduI GDGCHC 3 cut(s) 18, 512, 556
SfaNI GCATC 1 cut(s) 121
SfcI CTRYAG 1 cut(s) 442
SfoI GGCGCC 1 cut(s) 99
Sse9I AATT 4 cut(s) 190, 542, 671, 684
SseBI AGGCCT 1 cut(s) 345
SsiI CCGC 2 cut(s) 218, 642
SspDI GGCGCC 1 cut(s) 97
SspMI CTAG 2 cut(s) 359, 749
SstI GAGCTC 1 cut(s) 512
StuI AGGCCT 1 cut(s) 345
StyD4I CCNGG 4 cut(s) 4, 524, 610, 771
StyI CCWWGG 2 cut(s) 107, 176
TaaI ACNGT 3 cut(s) 316, 404, 806
TaiI ACGT 1 cut(s) 297
TaqI TCGA 6 cut(s) 130, 307, 416, 453, 558, 792
TasI AATT 4 cut(s) 190, 542, 671, 684
TauI GCSGC 1 cut(s) 645
TfiI GAWTC 3 cut(s) 413, 734, 821
Tru1I TTAA 6 cut(s) 171, 267, 579, 620, 674, 835
Tru9I TTAA 6 cut(s) 171, 267, 579, 620, 674, 835
TscAI CASTG 1 cut(s) 733
TseFI GTSAC 3 cut(s) 614, 767, 809
TseI GCWGC 4 cut(s) 285, 288, 639, 798
Tsp45I GTSAC 3 cut(s) 614, 767, 809
TspDTI ATGAA 1 cut(s) 238
TspGWI ACGGA 1 cut(s) 580
TspRI CASTG 1 cut(s) 733
XapI RAATTY 2 cut(s) 190, 684
XbaI TCTAGA 1 cut(s) 748
XmiI GTMKAC 1 cut(s) 59
XspI CTAG 2 cut(s) 359, 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.