Rroxscaffold_4G00288080

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0018043
Physical Location & Seq
Forward (+)
8987576 .. 8989320
1745 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00288080.1

Sequence Viewer

Length: 1257 bp
ATGGATCCATCGAGCAAGGAGAAGTATCGTCCCAATTTGGACACAGTTCTAGAGGTCCCTCACAAGATCACAGTTCTAAAGGTCCCTCGCAAGAACACAACAAAGTTATGGAACAGAATGAAATCATTGATGAACCCGAGGACACTGTCTCCCGATGTCATTGATGAACCCGATGGCAGGGCACTAGCTAGCTTACTTAGAGTTATTGGAGCTCCACTCGACCGCAACCTCATCCTAGGGCAATGGGATGAGGGGGCTGAATACATTGTGAAGCAATACGTGGAGGCGTTGGGCGGGGAGAAGGCTTTGTCATCAATGGACAGCTTGTGTGCGACGGGCAAGGTGCAGATCACAGTGTCAAATGGGACGAAAGGAAGCTTGAAATCCATTGGCGGTGGGGGAGGAGTCGGAGGACATGAAATGCCAGGGTTTGTGCTCTGGCAAAAGAGGGAGCTATGGTGCTTGGAAGTGATGTTGTCTACTGGTTACAAAGTTAGCACGGGGAGTGATGGCAAGGCGCCTTTGCCTTGGCATCACTCGTACGCTTATCGAGTCCCACCTCTTCCCCTCGGACACCTCCTTCAGGGTCTTAATCCAAGGTCAACAGCAAATTTATTCAGAAACTCCGTTTGTTTTGCGGACATACCAATAAACAAAGAGGGCTGCTTCAAACTACTACTGAAGGTTAAGCCTTCAACACTAAGCAGCAGCAACGTAGAGATAGTTGGACAAACCGTTATTGGCTACTTCAGCCACAAAACAGGCCTTTTGGTGAAGCTAGAAGACTCTCGCCAACTAAGGACGACAGCTTTCGGAAAGCACAGTTATTGGAAATCGACAATGGAGTCAGACTTCCAAGACTACAGAACCATCGACGGCCTCAACATCGCACACGGGGGTAGGACTTGTGTGTCTTTGTGTGGTGAGAGCTCGGAAGGCCATCCCCGGAGAAGAATAGTGAAATTGGTTTGGGCACTTGAAGAAGTAGGTTTTGACATTAAGGGGTTGTCCGTGGAGTGCTTCTTGCCTCCTGGTGACTTAAAGAAAGAGGAAGAAAGCTGCGGGATGGTGAAGTCTAAATCTTGTGCAAAATTAACATCCAGAATTCCATCTCCTTTGAGATTTCAAGCTACCAAAGTAGTTCCCAGTGATGATTCTAATCATGATCTAGAAGGTAGCGACATTAGTGACCAGGAGGATCTTCAAGATTTCGAGAGCTGCAACAGTAGTGACCATGATGATTCTGATCAATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

418

Amino Acids

46.01

Weight (kDa)

6.83

Isoelectric Point (pI)

42.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 110 - 344 2e-63 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018043)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g14750
rosa_chinensis RchiOBHm_Chr1g0369021
rosa_laevigata RLG00000027164
rosa_multiflora Rmu_sc0004250.1_g000030
rosa_roxburghii Rroxscaffold_4G00288080
rosa_rugosa Rorug01G0347000
rosa_samantha Rh1AG353900 Rh1BG317400 Rh1CG331900 Rh1DG348300
rosa_wichuraiana Rw1G031270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 844, 910
AccB1I GGYRCC 1 cut(s) 517
AccI GTMKAC 1 cut(s) 479
AciI CCGC 5 cut(s) 223, 294, 393, 638, 1062
AclWI GGATC 2 cut(s) 12, 1206
AcsI RAATTY 2 cut(s) 610, 1104
AcuI CTGAAG 3 cut(s) 566, 701, 733
AcyI GRCGYC 1 cut(s) 518
AfaI GTAC 1 cut(s) 542
AfiI CCNNNNNNNGG 2 cut(s) 177, 583
AgsI TTSAA 6 cut(s) 382, 670, 696, 980, 1127, 1205
AjnI CCWGG 3 cut(s) 424, 1030, 1191
Alw21I GWGCWC 3 cut(s) 214, 438, 932
Alw26I GTCTC 1 cut(s) 153
AlwI GGATC 2 cut(s) 12, 1206
Ama87I CYCGRG 1 cut(s) 136
AoxI GGCC 3 cut(s) 763, 877, 937
ApeKI GCWGC 5 cut(s) 663, 705, 708, 1059, 1218
ApoI RAATTY 2 cut(s) 610, 1104
AspA2I CCTAGG 1 cut(s) 235
AspLEI GCGC 1 cut(s) 520
AspS9I GGNCC 2 cut(s) 55, 82
AsuC2I CCSGG 1 cut(s) 946
AsuHPI GGTGA 4 cut(s) 784, 935, 1046, 1081
AsuNHI GCTAGC 1 cut(s) 188
AvaI CYCGRG 1 cut(s) 136
AvaII GGWCC 2 cut(s) 55, 82
AvrII CCTAGG 1 cut(s) 235
BaeGI GKGCMC 2 cut(s) 184, 976
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 517
BanII GRGCYC 2 cut(s) 214, 932
BbsI GAAGAC 1 cut(s) 789
Bbv12I GWGCWC 3 cut(s) 214, 438, 932
BbvI GCAGC 5 cut(s) 650, 717, 720, 1046, 1205
BccI CCATC 7 cut(s) 16, 167, 503, 878, 948, 1060, 1117
BceAI ACGGC 1 cut(s) 892
BciT130I CCWGG 3 cut(s) 426, 1032, 1193
BclI TGATCA 1 cut(s) 1246
BcnI CCSGG 1 cut(s) 946
BcoDI GTCTC 1 cut(s) 153
BfaI CTAG 6 cut(s) 50, 185, 189, 236, 779, 1169
BfmI CTRYAG 1 cut(s) 862
BfoI RGCGCY 1 cut(s) 521
BisI GCNGC 5 cut(s) 664, 706, 709, 1060, 1219
BlnI CCTAGG 1 cut(s) 235
BlsI GCNGC 5 cut(s) 665, 707, 710, 1061, 1220
Bme1390I CCNGG 4 cut(s) 426, 946, 1032, 1193
Bme18I GGWCC 2 cut(s) 55, 82
BmeT110I CYCGRG 1 cut(s) 136
BmgT120I GGNCC 2 cut(s) 55, 82
BmiI GGNNCC 4 cut(s) 6, 57, 84, 519
BmrFI CCNGG 4 cut(s) 426, 946, 1032, 1193
BmrI ACTGGG 1 cut(s) 1140
BmsI GCATC 1 cut(s) 541
BmtI GCTAGC 1 cut(s) 192
BmuI ACTGGG 1 cut(s) 1140
BpiI GAAGAC 1 cut(s) 789
BplI GAGNNNNNCTC 2 cut(s) 201, 233
BpuMI CCSGG 1 cut(s) 946
BsaAI YACGTR 1 cut(s) 280
BsaBI GATNNNNATC 2 cut(s) 1158, 1245
BsaHI GRCGYC 1 cut(s) 518
BsaJI CCNNGG 8 cut(s) 137, 235, 425, 527, 568, 596, 944, 1011
BsaXI ACNNNNNCTCC 4 cut(s) 133, 163, 443, 473
Bsc4I CCNNNNNNNGG 2 cut(s) 177, 583
Bse1I ACTGG 2 cut(s) 487, 1146
Bse3DI GCAATG 1 cut(s) 248
Bse8I GATNNNNATC 2 cut(s) 1158, 1245
BseBI CCWGG 3 cut(s) 426, 1032, 1193
BseDI CCNNGG 8 cut(s) 137, 235, 425, 527, 568, 596, 944, 1011
BseGI GGATG 5 cut(s) 231, 253, 940, 1071, 1097
BseJI GATNNNNATC 2 cut(s) 1158, 1245
BseLI CCNNNNNNNGG 2 cut(s) 177, 583
BseMI GCAATG 1 cut(s) 248
BseNI ACTGG 2 cut(s) 487, 1146
BseRI GAGGAG 1 cut(s) 417
BseSI GKGCMC 2 cut(s) 184, 976
BseXI GCAGC 5 cut(s) 650, 717, 720, 1046, 1205
BsgI GTGCAG 1 cut(s) 365
Bsh1285I CGRYCG 1 cut(s) 223
BshFI GGCC 3 cut(s) 765, 879, 939
BshNI GGYRCC 1 cut(s) 517
BsiEI CGRYCG 1 cut(s) 223
BsiHKAI GWGCWC 3 cut(s) 214, 438, 932
BsiHKCI CYCGRG 1 cut(s) 136
BsiSI CCGG 1 cut(s) 946
BsiWI CGTACG 1 cut(s) 540
BslFI GGGAC 5 cut(s) 15, 41, 68, 379, 539
BslI CCNNNNNNNGG 2 cut(s) 177, 583
BsmAI GTCTC 1 cut(s) 153
BsmFI GGGAC 5 cut(s) 15, 41, 68, 379, 539
BsnI GGCC 3 cut(s) 765, 879, 939
BsoBI CYCGRG 1 cut(s) 136
Bsp1286I GDGCHC 5 cut(s) 184, 214, 438, 932, 976
Bsp143I GATC 6 cut(s) 4, 66, 348, 1165, 1198, 1246
BspACI CCGC 5 cut(s) 223, 294, 393, 638, 1062
BspANI GGCC 3 cut(s) 765, 879, 939
BspHI TCATGA 1 cut(s) 1162
BspLI GGNNCC 4 cut(s) 6, 57, 84, 519
BspOI GCTAGC 1 cut(s) 192
BspPI GGATC 2 cut(s) 12, 1206
BspT107I GGYRCC 1 cut(s) 517
BsrDI GCAATG 1 cut(s) 248
BsrI ACTGG 2 cut(s) 487, 1146
BssECI CCNNGG 8 cut(s) 137, 235, 425, 527, 568, 596, 944, 1011
BssMI GATC 6 cut(s) 4, 66, 348, 1165, 1198, 1246
BssNI GRCGYC 1 cut(s) 518
BssT1I CCWWGG 3 cut(s) 235, 527, 596
Bst2UI CCWGG 3 cut(s) 426, 1032, 1193
Bst4CI ACNGT 7 cut(s) 46, 73, 147, 355, 736, 824, 1226
Bst6I CTCTTC 1 cut(s) 567
BstACI GRCGYC 1 cut(s) 518
BstBAI YACGTR 1 cut(s) 280
BstC8I GCNNGC 1 cut(s) 190
BstDEI CTNAG 3 cut(s) 197, 701, 797
BstDSI CCRYGG 1 cut(s) 1011
BstENI CCTNNNNNAGG 1 cut(s) 581
BstF5I GGATG 5 cut(s) 231, 253, 940, 1071, 1097
BstH2I RGCGCY 1 cut(s) 521
BstHHI GCGC 1 cut(s) 520
BstKTI GATC 6 cut(s) 7, 69, 351, 1168, 1201, 1249
BstMAI GTCTC 1 cut(s) 153
BstMBI GATC 6 cut(s) 4, 66, 348, 1165, 1198, 1246
BstMCI CGRYCG 1 cut(s) 223
BstMWI GCNNNNNNNGC 2 cut(s) 750, 936
BstNI CCWGG 3 cut(s) 426, 1032, 1193
BstSCI CCNGG 4 cut(s) 424, 944, 1030, 1191
BstSFI CTRYAG 1 cut(s) 862
BstSLI GKGCMC 2 cut(s) 184, 976
BstV1I GCAGC 5 cut(s) 650, 717, 720, 1046, 1205
BstV2I GAAGAC 1 cut(s) 789
BstX2I RGATCY 2 cut(s) 4, 1198
BstYI RGATCY 2 cut(s) 4, 1198
BsuRI GGCC 3 cut(s) 765, 879, 939
BtgI CCRYGG 1 cut(s) 1011
BtgZI GCGATG 1 cut(s) 871
BtsCI GGATG 5 cut(s) 231, 253, 940, 1071, 1097
BtsIMutI CAGTG 3 cut(s) 143, 360, 1153
Cac8I GCNNGC 1 cut(s) 190
CciI TCATGA 1 cut(s) 1162
CfoI GCGC 1 cut(s) 520
Cfr13I GGNCC 2 cut(s) 55, 82
Csp6I GTAC 1 cut(s) 541
CviAII CATG 3 cut(s) 416, 1163, 1235
CviQI GTAC 1 cut(s) 541
DdeI CTNAG 3 cut(s) 197, 701, 797
DinI GGCGCC 1 cut(s) 519
DpnI GATC 6 cut(s) 6, 68, 350, 1167, 1200, 1248
DpnII GATC 6 cut(s) 4, 66, 348, 1165, 1198, 1246
DrdI GACNNNNNNGTC 2 cut(s) 844, 910
DseDI GACNNNNNNGTC 2 cut(s) 844, 910
Eam1104I CTCTTC 1 cut(s) 567
EarI CTCTTC 1 cut(s) 567
Ecl136II GAGCTC 2 cut(s) 212, 930
Eco130I CCWWGG 3 cut(s) 235, 527, 596
Eco147I AGGCCT 1 cut(s) 765
Eco24I GRGCYC 2 cut(s) 214, 932
Eco47I GGWCC 2 cut(s) 55, 82
Eco53kI GAGCTC 2 cut(s) 212, 930
Eco57I CTGAAG 3 cut(s) 566, 701, 733
Eco88I CYCGRG 1 cut(s) 136
EcoICRI GAGCTC 2 cut(s) 212, 930
EcoNI CCTNNNNNAGG 1 cut(s) 581
EcoO109I RGGNCCY 2 cut(s) 55, 82
EcoRI GAATTC 1 cut(s) 1104
EcoRII CCWGG 3 cut(s) 424, 1030, 1191
EcoT14I CCWWGG 3 cut(s) 235, 527, 596
EcoT38I GRGCYC 2 cut(s) 214, 932
EgeI GGCGCC 1 cut(s) 519
EheI GGCGCC 1 cut(s) 519
ErhI CCWWGG 3 cut(s) 235, 527, 596
FaeI CATG 3 cut(s) 419, 1166, 1238
FaiI YATR 6 cut(s) 109, 417, 457, 644, 1164, 1236
FaqI GGGAC 5 cut(s) 15, 41, 68, 379, 539
FatI CATG 3 cut(s) 415, 1162, 1234
FauI CCCGC 2 cut(s) 287, 1055
FbaI TGATCA 1 cut(s) 1246
FblI GTMKAC 1 cut(s) 479
Fnu4HI GCNGC 5 cut(s) 664, 706, 709, 1060, 1219
FokI GGATG 5 cut(s) 218, 260, 927, 1078, 1084
FriOI GRGCYC 2 cut(s) 214, 932
Fsp4HI GCNGC 5 cut(s) 664, 706, 709, 1060, 1219
FspBI CTAG 6 cut(s) 50, 185, 189, 236, 779, 1169
GlaI GCGC 1 cut(s) 519
GluI GCNGC 5 cut(s) 664, 706, 709, 1060, 1219
HaeII RGCGCY 1 cut(s) 521
HaeIII GGCC 3 cut(s) 765, 879, 939
HapII CCGG 1 cut(s) 946
HhaI GCGC 1 cut(s) 520
Hin1I GRCGYC 1 cut(s) 518
Hin1II CATG 3 cut(s) 419, 1166, 1238
Hin6I GCGC 1 cut(s) 518
HinP1I GCGC 1 cut(s) 518
HincII GTYRAC 1 cut(s) 603
HindII GTYRAC 1 cut(s) 603
HindIII AAGCTT 1 cut(s) 376
HinfI GANTC 6 cut(s) 405, 552, 785, 845, 1154, 1241
HpaII CCGG 1 cut(s) 946
HphI GGTGA 4 cut(s) 784, 935, 1046, 1081
Hpy166II GTNNAC 2 cut(s) 480, 603
Hpy188I TCNGA 7 cut(s) 410, 572, 620, 815, 850, 934, 1246
Hpy188III TCNNGA 7 cut(s) 50, 152, 1101, 1163, 1169, 1205, 1213
Hpy8I GTNNAC 2 cut(s) 480, 603
Hpy99I CGWCG 2 cut(s) 337, 878
HpyAV CCTTC 6 cut(s) 295, 590, 676, 702, 929, 1166
HpyCH4III ACNGT 7 cut(s) 46, 73, 147, 355, 736, 824, 1226
HpyCH4IV ACGT 2 cut(s) 279, 714
HpyCH4V TGCA 3 cut(s) 346, 1088, 1221
HpyF10VI GCNNNNNNNGC 2 cut(s) 750, 936
HpyF3I CTNAG 3 cut(s) 197, 701, 797
HpySE526I ACGT 2 cut(s) 279, 714
Hsp92I GRCGYC 1 cut(s) 518
Hsp92II CATG 3 cut(s) 419, 1166, 1238
HspAI GCGC 1 cut(s) 518
KasI GGCGCC 1 cut(s) 517
Ksp22I TGATCA 1 cut(s) 1246
Kzo9I GATC 6 cut(s) 4, 66, 348, 1165, 1198, 1246
LmnI GCTCC 3 cut(s) 209, 217, 451
Lsp1109I GCAGC 5 cut(s) 650, 717, 720, 1046, 1205
LweI GCATC 1 cut(s) 541
MaeI CTAG 6 cut(s) 50, 185, 189, 236, 779, 1169
MaeII ACGT 2 cut(s) 279, 714
MaeIII GTNAC 4 cut(s) 485, 1034, 1187, 1229
MalI GATC 6 cut(s) 6, 68, 350, 1167, 1200, 1248
MboI GATC 6 cut(s) 4, 66, 348, 1165, 1198, 1246
MboII GAAGA 6 cut(s) 554, 794, 963, 992, 1064, 1193
MflI RGATCY 2 cut(s) 4, 1198
MhlI GDGCHC 5 cut(s) 184, 214, 438, 932, 976
MluCI AATT 5 cut(s) 34, 610, 962, 1091, 1104
Mly113I GGCGCC 1 cut(s) 518
MlyI GAGTC 4 cut(s) 414, 561, 779, 854
MmeI TCCRAC 2 cut(s) 388, 706
MseI TTAA 6 cut(s) 591, 687, 999, 1040, 1094, 1255
MspI CCGG 1 cut(s) 946
MspR9I CCNGG 4 cut(s) 426, 946, 1032, 1193
MvaI CCWGG 3 cut(s) 426, 1032, 1193
MwoI GCNNNNNNNGC 2 cut(s) 750, 936
NarI GGCGCC 1 cut(s) 518
NciI CCSGG 1 cut(s) 946
NdeII GATC 6 cut(s) 4, 66, 348, 1165, 1198, 1246
NheI GCTAGC 1 cut(s) 188
NlaIII CATG 3 cut(s) 419, 1166, 1238
NlaIV GGNNCC 4 cut(s) 6, 57, 84, 519
NmuCI GTSAC 3 cut(s) 1034, 1187, 1229
PagI TCATGA 1 cut(s) 1162
PceI AGGCCT 1 cut(s) 765
PfeI GAWTC 2 cut(s) 1154, 1241
Pfl23II CGTACG 1 cut(s) 540
PflFI GACNNNGTC 1 cut(s) 145
PkrI GCNGC 5 cut(s) 665, 707, 710, 1061, 1220
PleI GAGTC 4 cut(s) 413, 560, 779, 853
PluTI GGCGCC 1 cut(s) 521
PpsI GAGTC 4 cut(s) 413, 560, 779, 853
Ppu21I YACGTR 1 cut(s) 280
PpuMI RGGWCCY 2 cut(s) 55, 82
Psp124BI GAGCTC 2 cut(s) 214, 932
Psp5II RGGWCCY 2 cut(s) 55, 82
Psp6I CCWGG 3 cut(s) 424, 1030, 1191
PspGI CCWGG 3 cut(s) 424, 1030, 1191
PspLI CGTACG 1 cut(s) 540
PspN4I GGNNCC 4 cut(s) 6, 57, 84, 519
PspPI GGNCC 2 cut(s) 55, 82
PspPPI RGGWCCY 2 cut(s) 55, 82
PsuI RGATCY 2 cut(s) 4, 1198
PsyI GACNNNGTC 1 cut(s) 145
RsaI GTAC 1 cut(s) 542
RsaNI GTAC 1 cut(s) 541
SacI GAGCTC 2 cut(s) 214, 932
SaqAI TTAA 6 cut(s) 591, 687, 999, 1040, 1094, 1255
SatI GCNGC 5 cut(s) 664, 706, 709, 1060, 1219
Sau3AI GATC 6 cut(s) 4, 66, 348, 1165, 1198, 1246
Sau96I GGNCC 2 cut(s) 55, 82
SchI GAGTC 4 cut(s) 414, 561, 779, 854
ScrFI CCNGG 4 cut(s) 426, 946, 1032, 1193
SduI GDGCHC 5 cut(s) 184, 214, 438, 932, 976
SfaNI GCATC 1 cut(s) 541
SfcI CTRYAG 1 cut(s) 862
SfoI GGCGCC 1 cut(s) 519
SinI GGWCC 2 cut(s) 55, 82
Sse9I AATT 5 cut(s) 34, 610, 962, 1091, 1104
SseBI AGGCCT 1 cut(s) 765
SsiI CCGC 5 cut(s) 223, 294, 393, 638, 1062
SspDI GGCGCC 1 cut(s) 517
SspMI CTAG 6 cut(s) 50, 185, 189, 236, 779, 1169
SstI GAGCTC 2 cut(s) 214, 932
StuI AGGCCT 1 cut(s) 765
StyD4I CCNGG 4 cut(s) 424, 944, 1030, 1191
StyI CCWWGG 3 cut(s) 235, 527, 596
TaaI ACNGT 7 cut(s) 46, 73, 147, 355, 736, 824, 1226
TaiI ACGT 2 cut(s) 282, 717
TaqI TCGA 6 cut(s) 11, 219, 550, 836, 873, 1212
TasI AATT 5 cut(s) 34, 610, 962, 1091, 1104
TfiI GAWTC 2 cut(s) 1154, 1241
Tru1I TTAA 6 cut(s) 591, 687, 999, 1040, 1094, 1255
Tru9I TTAA 6 cut(s) 591, 687, 999, 1040, 1094, 1255
TscAI CASTG 3 cut(s) 150, 360, 1153
TseFI GTSAC 3 cut(s) 1034, 1187, 1229
TseI GCWGC 5 cut(s) 663, 705, 708, 1059, 1218
Tsp45I GTSAC 3 cut(s) 1034, 1187, 1229
TspDTI ATGAA 4 cut(s) 134, 146, 180, 432
TspGWI ACGGA 2 cut(s) 616, 1000
TspRI CASTG 3 cut(s) 150, 360, 1153
Tth111I GACNNNGTC 1 cut(s) 145
VpaK11BI GGWCC 2 cut(s) 55, 82
XagI CCTNNNNNAGG 1 cut(s) 581
XapI RAATTY 2 cut(s) 610, 1104
XbaI TCTAGA 2 cut(s) 49, 1168
XmaJI CCTAGG 1 cut(s) 235
XmiI GTMKAC 1 cut(s) 479
XspI CTAG 6 cut(s) 50, 185, 189, 236, 779, 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.