FvH4_5g17470

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0015326
Physical Location & Seq
Reverse (-)
10002325 .. 10003167
843 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g17470.t1

Sequence Viewer

Length: 534 bp
ATGGGGAATGCAGCAGCGTCTTGTGCTCCTTCAATACTCTCAAACAGTGCAACAGTCAAGGTCCTATTCTTCGATGGAAGATTGGAAGTCTACACAAGACCAATCAAAGCAGCAGAGCTAATGCTAGAGAATTCAGGCCATTTTGTGGTTGAATCTACCAGCCTCAAAGTAGGGAACAGAATCCATGGACTCACAGCCGATGAAGATCTCGAAAGACGAAACTTCTATTTCCTTCTTCCCATGGATTTACTCTACTCAGTTCTAACACATGAAGAACTCAGCTCCTTAACTTACAAGGCTTCAAGGGCACTCAAGCACAGAGGAAGCTTCAACAACTTCAGCAAGATTTTTCCGGTCCTCGGTGAGTTCTGTATGTTTCCCAACTCAGAATCCAAGACATTGGATGGTTCTGATCATCACAGTCTTGCAAGCCATTCGTTAGTGCCATTGTTAGAGAGGAGGTACTCAAAGCAAAGATCATGGAAGCCTGCATTGGAGACCATAGTTGAAACCAATACTCCATGTATACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

178

Amino Acids

19.86

Weight (kDa)

7.09

Isoelectric Point (pI)

34.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 170 2.9e-38 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015326)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18290
fragaria_vesca FvH4_5g17470
malus_domestica MD06G1228100.v1.1 MD14G1237300.v1.1
prunus_persica Prupe.5G234600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0178221
rosa_laevigata RLG00000005462
rosa_multiflora Rmu_co8411017.1_g000001 Rmu_sc0007855.1_g000004
rosa_roxburghii Rroxscaffold_3G00275070
rosa_rugosa Rorug06G0415100
rosa_samantha Rh7AG014200 Rh7BG013700 Rh7CG013600 Rh7DG013900
rosa_wichuraiana Rw7G001110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 145
AccI GTMKAC 2 cut(s) 90, 526
AcsI RAATTY 1 cut(s) 130
AcuI CTGAAG 1 cut(s) 322
AfaI GTAC 1 cut(s) 464
AfiI CCNNNNNNNGG 2 cut(s) 145, 359
AgsI TTSAA 5 cut(s) 33, 152, 303, 331, 509
AjuI GAANNNNNNNTTGG 2 cut(s) 476, 508
AluBI AGCT 3 cut(s) 118, 282, 327
AluI AGCT 3 cut(s) 118, 282, 327
Alw21I GWGCWC 1 cut(s) 28
Alw26I GTCTC 1 cut(s) 491
AoxI GGCC 1 cut(s) 136
ApeKI GCWGC 3 cut(s) 11, 14, 110
ApoI RAATTY 1 cut(s) 130
AspS9I GGNCC 2 cut(s) 61, 355
AsuHPI GGTGA 1 cut(s) 374
AvaII GGWCC 2 cut(s) 61, 355
BaeGI GKGCMC 1 cut(s) 310
Bbv12I GWGCWC 1 cut(s) 28
BbvI GCAGC 3 cut(s) 23, 26, 122
BccI CCATC 2 cut(s) 68, 398
BcgI CGANNNNNNTGC 2 cut(s) 417, 451
BclI TGATCA 1 cut(s) 412
BcoDI GTCTC 1 cut(s) 491
BfaI CTAG 1 cut(s) 125
BglII AGATCT 1 cut(s) 205
BisI GCNGC 3 cut(s) 12, 15, 111
BlsI GCNGC 3 cut(s) 13, 16, 112
Bme18I GGWCC 2 cut(s) 61, 355
BmgT120I GGNCC 2 cut(s) 61, 355
BpuEI CTTGAG 1 cut(s) 296
BsaBI GATNNNNATC 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 491
BsaJI CCNNGG 3 cut(s) 184, 240, 358
BsaWI WCCGGW 1 cut(s) 352
BsaXI ACNNNNNCTCC 2 cut(s) 502, 532
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 359
Bse8I GATNNNNATC 1 cut(s) 204
BseDI CCNNGG 3 cut(s) 184, 240, 358
BseGI GGATG 1 cut(s) 409
BseJI GATNNNNATC 1 cut(s) 204
BseLI CCNNNNNNNGG 2 cut(s) 145, 359
BseMII CTCAG 3 cut(s) 270, 292, 399
BseRI GAGGAG 1 cut(s) 472
BseSI GKGCMC 1 cut(s) 310
BseXI GCAGC 3 cut(s) 23, 26, 122
BshFI GGCC 1 cut(s) 138
BsiHKAI GWGCWC 1 cut(s) 28
BsiSI CCGG 1 cut(s) 353
BslI CCNNNNNNNGG 2 cut(s) 145, 359
BsmAI GTCTC 1 cut(s) 491
BsmI GAATGC 1 cut(s) 13
BsnI GGCC 1 cut(s) 138
Bso31I GGTCTC 1 cut(s) 491
Bsp1286I GDGCHC 2 cut(s) 28, 310
Bsp143I GATC 3 cut(s) 205, 412, 476
Bsp19I CCATGG 2 cut(s) 184, 240
BspANI GGCC 1 cut(s) 138
BspCNI CTCAG 3 cut(s) 269, 291, 398
BspTNI GGTCTC 1 cut(s) 491
BssECI CCNNGG 3 cut(s) 184, 240, 358
BssMI GATC 3 cut(s) 205, 412, 476
BssNAI GTATAC 1 cut(s) 527
BssT1I CCWWGG 2 cut(s) 184, 240
Bst1107I GTATAC 1 cut(s) 527
Bst4CI ACNGT 3 cut(s) 47, 55, 422
BstC8I GCNNGC 2 cut(s) 430, 489
BstDEI CTNAG 3 cut(s) 256, 278, 385
BstDSI CCRYGG 2 cut(s) 184, 240
BstF5I GGATG 1 cut(s) 409
BstKTI GATC 3 cut(s) 208, 415, 479
BstMAI GTCTC 1 cut(s) 491
BstMBI GATC 3 cut(s) 205, 412, 476
BstMWI GCNNNNNNNGC 2 cut(s) 23, 305
BstSLI GKGCMC 1 cut(s) 310
BstV1I GCAGC 3 cut(s) 23, 26, 122
BstX2I RGATCY 1 cut(s) 205
BstXI CCANNNNNNTGG 1 cut(s) 400
BstYI RGATCY 1 cut(s) 205
BstZ17I GTATAC 1 cut(s) 527
BsuRI GGCC 1 cut(s) 138
BtgI CCRYGG 2 cut(s) 184, 240
BtsCI GGATG 1 cut(s) 409
BtsIMutI CAGTG 1 cut(s) 52
Cac8I GCNNGC 2 cut(s) 430, 489
Cfr13I GGNCC 2 cut(s) 61, 355
CseI GACGC 1 cut(s) 6
Csp6I GTAC 1 cut(s) 463
CviAII CATG 5 cut(s) 185, 241, 269, 480, 522
CviJI RGCY 9 cut(s) 118, 138, 162, 197, 282, 299, 327, 432, 487
CviKI_1 RGCY 9 cut(s) 118, 138, 162, 197, 282, 299, 327, 432, 487
CviQI GTAC 1 cut(s) 463
DdeI CTNAG 3 cut(s) 256, 278, 385
DpnI GATC 3 cut(s) 207, 414, 478
DpnII GATC 3 cut(s) 205, 412, 476
Eco130I CCWWGG 2 cut(s) 184, 240
Eco31I GGTCTC 1 cut(s) 491
Eco47I GGWCC 2 cut(s) 61, 355
Eco57I CTGAAG 1 cut(s) 322
EcoO109I RGGNCCY 1 cut(s) 61
EcoRI GAATTC 1 cut(s) 130
EcoT14I CCWWGG 2 cut(s) 184, 240
ErhI CCWWGG 2 cut(s) 184, 240
FaeI CATG 5 cut(s) 188, 244, 272, 483, 525
FaiI YATR 8 cut(s) 186, 242, 270, 374, 481, 503, 523, 527
FatI CATG 5 cut(s) 184, 240, 268, 479, 521
FbaI TGATCA 1 cut(s) 412
FblI GTMKAC 2 cut(s) 90, 526
Fnu4HI GCNGC 3 cut(s) 12, 15, 111
FokI GGATG 1 cut(s) 416
Fsp4HI GCNGC 3 cut(s) 12, 15, 111
FspBI CTAG 1 cut(s) 125
GluI GCNGC 3 cut(s) 12, 15, 111
HaeIII GGCC 1 cut(s) 138
HapII CCGG 1 cut(s) 353
HgaI GACGC 1 cut(s) 6
Hin1II CATG 5 cut(s) 188, 244, 272, 483, 525
HindIII AAGCTT 1 cut(s) 325
HinfI GANTC 4 cut(s) 152, 180, 189, 389
HpaII CCGG 1 cut(s) 353
HphI GGTGA 1 cut(s) 374
Hpy166II GTNNAC 2 cut(s) 91, 527
Hpy188I TCNGA 2 cut(s) 388, 412
Hpy188III TCNNGA 1 cut(s) 209
Hpy8I GTNNAC 2 cut(s) 91, 527
HpyAV CCTTC 2 cut(s) 39, 242
HpyCH4III ACNGT 3 cut(s) 47, 55, 422
HpyCH4V TGCA 4 cut(s) 11, 50, 428, 491
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 305
HpyF3I CTNAG 3 cut(s) 256, 278, 385
Hsp92II CATG 5 cut(s) 188, 244, 272, 483, 525
Ksp22I TGATCA 1 cut(s) 412
Kzo9I GATC 3 cut(s) 205, 412, 476
LmnI GCTCC 2 cut(s) 31, 287
LpnPI CCDG 4 cut(s) 120, 172, 366, 501
Lsp1109I GCAGC 3 cut(s) 23, 26, 122
MaeI CTAG 1 cut(s) 125
MalI GATC 3 cut(s) 207, 414, 478
MboI GATC 3 cut(s) 205, 412, 476
MboII GAAGA 5 cut(s) 61, 90, 215, 227, 284
MflI RGATCY 1 cut(s) 205
MhlI GDGCHC 2 cut(s) 28, 310
MluCI AATT 1 cut(s) 130
MlyI GAGTC 1 cut(s) 183
MnlI CCTC 5 cut(s) 173, 314, 368, 450, 453
MseI TTAA 1 cut(s) 287
MspI CCGG 1 cut(s) 353
Mva1269I GAATGC 1 cut(s) 13
MwoI GCNNNNNNNGC 2 cut(s) 23, 305
NcoI CCATGG 2 cut(s) 184, 240
NdeII GATC 3 cut(s) 205, 412, 476
NlaIII CATG 5 cut(s) 188, 244, 272, 483, 525
PctI GAATGC 1 cut(s) 13
PfeI GAWTC 3 cut(s) 152, 180, 389
PflMI CCANNNNNTGG 1 cut(s) 145
PkrI GCNGC 3 cut(s) 13, 16, 112
PleI GAGTC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 183
PpuMI RGGWCCY 1 cut(s) 61
Psp5II RGGWCCY 1 cut(s) 61
PspPI GGNCC 2 cut(s) 61, 355
PspPPI RGGWCCY 1 cut(s) 61
PsuI RGATCY 1 cut(s) 205
RsaI GTAC 1 cut(s) 464
RsaNI GTAC 1 cut(s) 463
SaqAI TTAA 1 cut(s) 287
SatI GCNGC 3 cut(s) 12, 15, 111
Sau3AI GATC 3 cut(s) 205, 412, 476
Sau96I GGNCC 2 cut(s) 61, 355
SchI GAGTC 1 cut(s) 183
SduI GDGCHC 2 cut(s) 28, 310
SetI ASST 5 cut(s) 63, 120, 284, 329, 464
SinI GGWCC 2 cut(s) 61, 355
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
Sse9I AATT 1 cut(s) 130
SspMI CTAG 1 cut(s) 125
StyI CCWWGG 2 cut(s) 184, 240
TaaI ACNGT 3 cut(s) 47, 55, 422
TaqI TCGA 2 cut(s) 72, 210
TasI AATT 1 cut(s) 130
TfiI GAWTC 3 cut(s) 152, 180, 389
Tru1I TTAA 1 cut(s) 287
Tru9I TTAA 1 cut(s) 287
TscAI CASTG 1 cut(s) 52
TseI GCWGC 3 cut(s) 11, 14, 110
TspDTI ATGAA 2 cut(s) 216, 285
TspRI CASTG 1 cut(s) 52
Van91I CCANNNNNTGG 1 cut(s) 145
VpaK11BI GGWCC 2 cut(s) 61, 355
XapI RAATTY 1 cut(s) 130
XmiI GTMKAC 2 cut(s) 90, 526
XspI CTAG 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.