Rmu_sc0007855.1_g000004

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007855.1
OG0015326
Physical Location & Seq
Reverse (-)
22001 .. 22534
534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0007855.1_g000004.1.cds

Sequence Viewer

Length: 534 bp
atgggaaatgcagcagcttcttgtgttccttcaagactctcaaacagtacaacagtcaaggtccgattcttcgatggaagattggaagtctacatcagaccaatcaaagcagcagagctaatgctagagcattcaggccagtttgtggttgagtctagcagcctcagagtagggaacagaatccatggccttacagccgatgaagaactggaaagacgaagattctactttcttcttcccatggatttgctctactcagttctcacacatgaagaactcagcgccctaacttacaaggcttcaagggcactcaagcacagaggaagcttcaacaacttcagcaagatttttccggtcctcagcgagttttttatgtttcccaactcagaatcaaagacattggatggttctgatcatcacagtcctgcaagcaattcgttagtgccattgttggagaggaggtactcaaagcaaagatcatggaagcctgcattggagaccattgttgaaaccagtactacatgtccacattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

177

Amino Acids

20.08

Weight (kDa)

8.95

Isoelectric Point (pI)

47.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015326)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18290
fragaria_vesca FvH4_5g17470
malus_domestica MD06G1228100.v1.1 MD14G1237300.v1.1
prunus_persica Prupe.5G234600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0178221
rosa_laevigata RLG00000005462
rosa_multiflora Rmu_co8411017.1_g000001 Rmu_sc0007855.1_g000004
rosa_roxburghii Rroxscaffold_3G00275070
rosa_rugosa Rorug06G0415100
rosa_samantha Rh7AG014200 Rh7BG013700 Rh7CG013600 Rh7DG013900
rosa_wichuraiana Rw7G001110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 145
AccI GTMKAC 1 cut(s) 90
AcuI CTGAAG 1 cut(s) 322
AfaI GTAC 3 cut(s) 49, 464, 517
AfiI CCNNNNNNNGG 1 cut(s) 145
AflIII ACRYGT 1 cut(s) 521
AgsI TTSAA 4 cut(s) 33, 303, 331, 509
AjuI GAANNNNNNNTTGG 2 cut(s) 476, 508
AluBI AGCT 3 cut(s) 17, 118, 327
AluI AGCT 3 cut(s) 17, 118, 327
Alw26I GTCTC 1 cut(s) 491
AoxI GGCC 2 cut(s) 136, 187
ApeKI GCWGC 4 cut(s) 11, 14, 110, 159
AspLEI GCGC 1 cut(s) 284
AspS9I GGNCC 2 cut(s) 61, 355
AvaII GGWCC 2 cut(s) 61, 355
BaeGI GKGCMC 1 cut(s) 310
BbvCI CCTCAGC 1 cut(s) 359
BbvI GCAGC 4 cut(s) 23, 26, 122, 171
BccI CCATC 2 cut(s) 68, 398
BcgI CGANNNNNNTGC 2 cut(s) 417, 451
BclI TGATCA 1 cut(s) 412
BcoDI GTCTC 1 cut(s) 491
BfaI CTAG 2 cut(s) 125, 156
BfoI RGCGCY 1 cut(s) 285
BisI GCNGC 4 cut(s) 12, 15, 111, 160
BlsI GCNGC 4 cut(s) 13, 16, 112, 161
BmcAI AGTACT 1 cut(s) 517
Bme18I GGWCC 2 cut(s) 61, 355
BmgT120I GGNCC 2 cut(s) 61, 355
Bpu10I CCTNAGC 1 cut(s) 359
BpuEI CTTGAG 1 cut(s) 296
BsaI GGTCTC 1 cut(s) 491
BsaJI CCNNGG 2 cut(s) 184, 240
BsaWI WCCGGW 1 cut(s) 352
BsaXI ACNNNNNCTCC 2 cut(s) 446, 476
Bsc4I CCNNNNNNNGG 1 cut(s) 145
Bse1I ACTGG 3 cut(s) 139, 213, 513
BseDI CCNNGG 2 cut(s) 184, 240
BseGI GGATG 1 cut(s) 409
BseLI CCNNNNNNNGG 1 cut(s) 145
BseMII CTCAG 5 cut(s) 178, 270, 292, 373, 399
BseNI ACTGG 3 cut(s) 139, 213, 513
BseRI GAGGAG 1 cut(s) 472
BseSI GKGCMC 1 cut(s) 310
BseXI GCAGC 4 cut(s) 23, 26, 122, 171
BshFI GGCC 2 cut(s) 138, 189
BsiSI CCGG 1 cut(s) 353
BslI CCNNNNNNNGG 1 cut(s) 145
BsmAI GTCTC 1 cut(s) 491
BsmI GAATGC 1 cut(s) 130
BsnI GGCC 2 cut(s) 138, 189
Bso31I GGTCTC 1 cut(s) 491
Bsp1286I GDGCHC 1 cut(s) 310
Bsp143I GATC 2 cut(s) 412, 476
Bsp19I CCATGG 2 cut(s) 184, 240
BspANI GGCC 2 cut(s) 138, 189
BspCNI CTCAG 5 cut(s) 177, 269, 291, 372, 398
BspTNI GGTCTC 1 cut(s) 491
BsrI ACTGG 3 cut(s) 139, 213, 513
BssECI CCNNGG 2 cut(s) 184, 240
BssMI GATC 2 cut(s) 412, 476
BssT1I CCWWGG 2 cut(s) 184, 240
Bst4CI ACNGT 3 cut(s) 47, 55, 422
BstC8I GCNNGC 2 cut(s) 430, 489
BstDEI CTNAG 5 cut(s) 164, 256, 278, 359, 385
BstDSI CCRYGG 2 cut(s) 184, 240
BstF5I GGATG 1 cut(s) 409
BstH2I RGCGCY 1 cut(s) 285
BstHHI GCGC 1 cut(s) 284
BstKTI GATC 2 cut(s) 415, 479
BstMAI GTCTC 1 cut(s) 491
BstMBI GATC 2 cut(s) 412, 476
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstNSI RCATGY 1 cut(s) 525
BstSLI GKGCMC 1 cut(s) 310
BstV1I GCAGC 4 cut(s) 23, 26, 122, 171
BsuRI GGCC 2 cut(s) 138, 189
BtgI CCRYGG 2 cut(s) 184, 240
BtsCI GGATG 1 cut(s) 409
Cac8I GCNNGC 2 cut(s) 430, 489
CfoI GCGC 1 cut(s) 284
Cfr13I GGNCC 2 cut(s) 61, 355
Csp6I GTAC 3 cut(s) 48, 463, 516
CviAII CATG 5 cut(s) 185, 241, 269, 480, 522
CviJI RGCY 9 cut(s) 17, 118, 138, 162, 189, 197, 299, 327, 487
CviKI_1 RGCY 9 cut(s) 17, 118, 138, 162, 189, 197, 299, 327, 487
CviQI GTAC 3 cut(s) 48, 463, 516
DdeI CTNAG 5 cut(s) 164, 256, 278, 359, 385
DpnI GATC 2 cut(s) 414, 478
DpnII GATC 2 cut(s) 412, 476
Eco130I CCWWGG 2 cut(s) 184, 240
Eco31I GGTCTC 1 cut(s) 491
Eco47I GGWCC 2 cut(s) 61, 355
Eco57I CTGAAG 1 cut(s) 322
EcoT14I CCWWGG 2 cut(s) 184, 240
ErhI CCWWGG 2 cut(s) 184, 240
FaeI CATG 5 cut(s) 188, 244, 272, 483, 525
FaiI YATR 6 cut(s) 186, 242, 270, 374, 481, 523
FatI CATG 5 cut(s) 184, 240, 268, 479, 521
FbaI TGATCA 1 cut(s) 412
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 4 cut(s) 12, 15, 111, 160
FokI GGATG 1 cut(s) 416
Fsp4HI GCNGC 4 cut(s) 12, 15, 111, 160
FspBI CTAG 2 cut(s) 125, 156
GlaI GCGC 1 cut(s) 283
GluI GCNGC 4 cut(s) 12, 15, 111, 160
HaeII RGCGCY 1 cut(s) 285
HaeIII GGCC 2 cut(s) 138, 189
HapII CCGG 1 cut(s) 353
HhaI GCGC 1 cut(s) 284
Hin1II CATG 5 cut(s) 188, 244, 272, 483, 525
Hin6I GCGC 1 cut(s) 282
HinP1I GCGC 1 cut(s) 282
HindIII AAGCTT 1 cut(s) 325
HinfI GANTC 6 cut(s) 36, 66, 152, 180, 222, 389
HpaII CCGG 1 cut(s) 353
Hpy166II GTNNAC 2 cut(s) 91, 527
Hpy188I TCNGA 5 cut(s) 65, 98, 167, 388, 412
Hpy188III TCNNGA 1 cut(s) 33
Hpy8I GTNNAC 2 cut(s) 91, 527
HpyAV CCTTC 1 cut(s) 39
HpyCH4III ACNGT 3 cut(s) 47, 55, 422
HpyCH4V TGCA 3 cut(s) 11, 428, 491
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpyF3I CTNAG 5 cut(s) 164, 256, 278, 359, 385
Hsp92II CATG 5 cut(s) 188, 244, 272, 483, 525
HspAI GCGC 1 cut(s) 282
Ksp22I TGATCA 1 cut(s) 412
Kzo9I GATC 2 cut(s) 412, 476
LpnPI CCDG 7 cut(s) 120, 152, 194, 366, 438, 501, 526
Lsp1109I GCAGC 4 cut(s) 23, 26, 122, 171
MaeI CTAG 2 cut(s) 125, 156
MalI GATC 2 cut(s) 414, 478
MboI GATC 2 cut(s) 412, 476
MboII GAAGA 7 cut(s) 61, 90, 215, 224, 227, 231, 284
MhlI GDGCHC 1 cut(s) 310
MluCI AATT 1 cut(s) 433
MlyI GAGTC 2 cut(s) 30, 161
MmeI TCCRAC 1 cut(s) 432
MnlI CCTC 5 cut(s) 173, 314, 368, 450, 453
MspI CCGG 1 cut(s) 353
Mva1269I GAATGC 1 cut(s) 130
MwoI GCNNNNNNNGC 1 cut(s) 305
NcoI CCATGG 2 cut(s) 184, 240
NdeII GATC 2 cut(s) 412, 476
NlaIII CATG 5 cut(s) 188, 244, 272, 483, 525
NspI RCATGY 1 cut(s) 525
PciI ACATGT 1 cut(s) 521
PctI GAATGC 1 cut(s) 130
PfeI GAWTC 4 cut(s) 66, 180, 222, 389
PflMI CCANNNNNTGG 1 cut(s) 145
PkrI GCNGC 4 cut(s) 13, 16, 112, 161
PleI GAGTC 2 cut(s) 30, 160
PpsI GAGTC 2 cut(s) 30, 160
PscI ACATGT 1 cut(s) 521
PspPI GGNCC 2 cut(s) 61, 355
RsaI GTAC 3 cut(s) 49, 464, 517
RsaNI GTAC 3 cut(s) 48, 463, 516
SatI GCNGC 4 cut(s) 12, 15, 111, 160
Sau3AI GATC 2 cut(s) 412, 476
Sau96I GGNCC 2 cut(s) 61, 355
ScaI AGTACT 1 cut(s) 517
SchI GAGTC 2 cut(s) 30, 161
SduI GDGCHC 1 cut(s) 310
SetI ASST 5 cut(s) 19, 63, 120, 329, 464
SinI GGWCC 2 cut(s) 61, 355
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
Sse9I AATT 1 cut(s) 433
SspMI CTAG 2 cut(s) 125, 156
StyI CCWWGG 2 cut(s) 184, 240
TaaI ACNGT 3 cut(s) 47, 55, 422
TaqI TCGA 1 cut(s) 72
TasI AATT 1 cut(s) 433
TatI WGTACW 2 cut(s) 47, 515
TfiI GAWTC 4 cut(s) 66, 180, 222, 389
TseI GCWGC 4 cut(s) 11, 14, 110, 159
TspDTI ATGAA 2 cut(s) 216, 285
Van91I CCANNNNNTGG 1 cut(s) 145
VpaK11BI GGWCC 2 cut(s) 61, 355
XceI RCATGY 1 cut(s) 525
XmiI GTMKAC 1 cut(s) 90
XspI CTAG 2 cut(s) 125, 156
ZrmI AGTACT 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.