MD06G1228100.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0015326
Physical Location & Seq
Reverse (-)
35829543 .. 35830094
552 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1228100.v1.1.491

Sequence Viewer

Length: 552 bp
ATGGGAAATGCAGCAGTTTCTTGTGCTCCATTGACCATATGCTCAAGCAGTCCAGGAGGAGCAACAAAAGTCTTATGTTTGGACGGAAGACTGGAAGTTTTCGGAAGGCAAGTAAAAGCATCAGAGCTAATGGTAGAGCATCCGGGCCAGTTTTTGTGTGACTCTACCATCCTCAAAGTTGGCAACAGAATCCACGGCCTCACTGCCGACGAACCGCTCGAAAGACGGCGGTTTTACTTCCTCCTCCCCATGGACCTCCTCTACTCAGTGCTAACACACGAAGAACTCAGCTCCCTAACTTACAGGGCTTCAAGGGCACTCAAGAACAGTAGAGGCTTCAACAATTTCAGTAGGATTTTTCCGGTTTTTGGAGATTTTCGCATGTTTAATCTTTCGGAATCCAAGAGATTGGATTGCAATGAAGATCATGCAATTATTAGTGTTAATTCAAGCCATAATTCAGAGCCAGTAGTATTATTGGAGAGGTACTCAAAGCAAAGATCATGGAAGCCTGCATTAGAAACCATTGATGAAACTCCTTGTACACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

184

Amino Acids

20.54

Weight (kDa)

6.95

Isoelectric Point (pI)

49.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 178 2.2e-33 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015326)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18290
fragaria_vesca FvH4_5g17470
malus_domestica MD06G1228100.v1.1 MD14G1237300.v1.1
prunus_persica Prupe.5G234600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0178221
rosa_laevigata RLG00000005462
rosa_multiflora Rmu_co8411017.1_g000001 Rmu_sc0007855.1_g000004
rosa_roxburghii Rroxscaffold_3G00275070
rosa_rugosa Rorug06G0415100
rosa_samantha Rh7AG014200 Rh7BG013700 Rh7CG013600 Rh7DG013900
rosa_wichuraiana Rw7G001110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 217
AciI CCGC 2 cut(s) 215, 229
AfaI GTAC 2 cut(s) 488, 544
AfiI CCNNNNNNNGG 2 cut(s) 250, 368
AgsI TTSAA 3 cut(s) 312, 340, 450
AjnI CCWGG 1 cut(s) 52
AluBI AGCT 2 cut(s) 127, 291
AluI AGCT 2 cut(s) 127, 291
Alw21I GWGCWC 1 cut(s) 28
AoxI GGCC 2 cut(s) 145, 196
ApeKI GCWGC 1 cut(s) 11
AspS9I GGNCC 2 cut(s) 145, 253
AsuC2I CCSGG 1 cut(s) 144
AvaII GGWCC 1 cut(s) 253
BaeGI GKGCMC 1 cut(s) 319
BbsI GAAGAC 1 cut(s) 94
Bbv12I GWGCWC 1 cut(s) 28
BbvI GCAGC 1 cut(s) 23
BccI CCATC 1 cut(s) 176
BceAI ACGGC 2 cut(s) 211, 242
BciT130I CCWGG 1 cut(s) 54
BcnI CCSGG 1 cut(s) 144
BisI GCNGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 13
Bme1390I CCNGG 2 cut(s) 54, 144
Bme18I GGWCC 1 cut(s) 253
BmgT120I GGNCC 2 cut(s) 145, 253
BmrFI CCNGG 2 cut(s) 54, 144
BmsI GCATC 2 cut(s) 128, 148
BpiI GAAGAC 1 cut(s) 94
BplI GAGNNNNNCTC 2 cut(s) 473, 505
BpuEI CTTGAG 2 cut(s) 28, 305
BpuMI CCSGG 1 cut(s) 144
BsaJI CCNNGG 2 cut(s) 193, 249
BsaWI WCCGGW 1 cut(s) 361
Bsc4I CCNNNNNNNGG 2 cut(s) 250, 368
Bse1I ACTGG 3 cut(s) 96, 148, 467
Bse3DI GCAATG 1 cut(s) 424
BseBI CCWGG 1 cut(s) 54
BseDI CCNNGG 2 cut(s) 193, 249
BseGI GGATG 2 cut(s) 139, 168
BseLI CCNNNNNNNGG 2 cut(s) 250, 368
BseMI GCAATG 1 cut(s) 424
BseMII CTCAG 2 cut(s) 279, 301
BseNI ACTGG 3 cut(s) 96, 148, 467
BseRI GAGGAG 3 cut(s) 72, 233, 248
BseSI GKGCMC 1 cut(s) 319
BseXI GCAGC 1 cut(s) 23
BshFI GGCC 2 cut(s) 147, 198
BsiHKAI GWGCWC 1 cut(s) 28
BsiSI CCGG 2 cut(s) 143, 362
BslI CCNNNNNNNGG 2 cut(s) 250, 368
BsnI GGCC 2 cut(s) 147, 198
Bsp1286I GDGCHC 2 cut(s) 28, 319
Bsp1407I TGTACA 1 cut(s) 542
Bsp143I GATC 2 cut(s) 424, 500
Bsp19I CCATGG 1 cut(s) 249
BspACI CCGC 2 cut(s) 215, 229
BspANI GGCC 2 cut(s) 147, 198
BspCNI CTCAG 2 cut(s) 278, 300
BsrBI CCGCTC 1 cut(s) 217
BsrDI GCAATG 1 cut(s) 424
BsrGI TGTACA 1 cut(s) 542
BsrI ACTGG 3 cut(s) 96, 148, 467
BssECI CCNNGG 2 cut(s) 193, 249
BssMI GATC 2 cut(s) 424, 500
BssT1I CCWWGG 1 cut(s) 249
Bst2UI CCWGG 1 cut(s) 54
Bst4CI ACNGT 1 cut(s) 329
BstAUI TGTACA 1 cut(s) 542
BstC8I GCNNGC 1 cut(s) 513
BstDEI CTNAG 2 cut(s) 265, 287
BstDSI CCRYGG 2 cut(s) 193, 249
BstF5I GGATG 2 cut(s) 139, 168
BstKTI GATC 2 cut(s) 427, 503
BstMBI GATC 2 cut(s) 424, 500
BstMWI GCNNNNNNNGC 1 cut(s) 314
BstNI CCWGG 1 cut(s) 54
BstNSI RCATGY 1 cut(s) 385
BstSCI CCNGG 2 cut(s) 52, 142
BstSLI GKGCMC 1 cut(s) 319
BstV1I GCAGC 1 cut(s) 23
BstV2I GAAGAC 1 cut(s) 94
BstXI CCANNNNNNTGG 1 cut(s) 409
BsuRI GGCC 2 cut(s) 147, 198
BtgI CCRYGG 2 cut(s) 193, 249
BtsCI GGATG 2 cut(s) 139, 168
BtsI GCAGTG 1 cut(s) 201
BtsIMutI CAGTG 2 cut(s) 201, 273
Cac8I GCNNGC 1 cut(s) 513
Cfr13I GGNCC 2 cut(s) 145, 253
Csp6I GTAC 2 cut(s) 487, 543
CviAII CATG 4 cut(s) 250, 382, 428, 504
CviJI RGCY 9 cut(s) 127, 147, 198, 291, 308, 336, 453, 466, 511
CviKI_1 RGCY 9 cut(s) 127, 147, 198, 291, 308, 336, 453, 466, 511
CviQI GTAC 2 cut(s) 487, 543
DdeI CTNAG 2 cut(s) 265, 287
DpnI GATC 2 cut(s) 426, 502
DpnII GATC 2 cut(s) 424, 500
Eco130I CCWWGG 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 253
EcoRII CCWGG 1 cut(s) 52
EcoT14I CCWWGG 1 cut(s) 249
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 4 cut(s) 253, 385, 431, 507
FaiI YATR 8 cut(s) 38, 40, 76, 251, 383, 429, 456, 505
FatI CATG 4 cut(s) 249, 381, 427, 503
FauNDI CATATG 1 cut(s) 38
Fnu4HI GCNGC 1 cut(s) 12
FokI GGATG 2 cut(s) 126, 155
Fsp4HI GCNGC 1 cut(s) 12
GluI GCNGC 1 cut(s) 12
HaeIII GGCC 2 cut(s) 147, 198
HapII CCGG 2 cut(s) 143, 362
Hin1II CATG 4 cut(s) 253, 385, 431, 507
HinfI GANTC 3 cut(s) 161, 189, 398
HpaII CCGG 2 cut(s) 143, 362
Hpy166II GTNNAC 1 cut(s) 545
Hpy188I TCNGA 4 cut(s) 104, 124, 397, 463
Hpy188III TCNNGA 1 cut(s) 322
Hpy8I GTNNAC 1 cut(s) 545
Hpy99I CGWCG 1 cut(s) 212
HpyAV CCTTC 1 cut(s) 99
HpyCH4III ACNGT 1 cut(s) 329
HpyCH4V TGCA 4 cut(s) 11, 417, 431, 515
HpyF10VI GCNNNNNNNGC 1 cut(s) 314
HpyF3I CTNAG 2 cut(s) 265, 287
Hsp92II CATG 4 cut(s) 253, 385, 431, 507
Kzo9I GATC 2 cut(s) 424, 500
LmnI GCTCC 3 cut(s) 31, 59, 296
LpnPI CCDG 9 cut(s) 39, 66, 77, 156, 161, 289, 375, 480, 525
Lsp1109I GCAGC 1 cut(s) 23
LweI GCATC 2 cut(s) 128, 148
MaeIII GTNAC 1 cut(s) 158
MalI GATC 2 cut(s) 426, 502
MbiI CCGCTC 1 cut(s) 217
MboI GATC 2 cut(s) 424, 500
MboII GAAGA 3 cut(s) 99, 293, 434
MhlI GDGCHC 2 cut(s) 28, 319
MluCI AATT 4 cut(s) 343, 432, 445, 457
MlyI GAGTC 1 cut(s) 155
MnlI CCTC 9 cut(s) 50, 182, 209, 251, 254, 266, 269, 326, 477
MseI TTAA 2 cut(s) 387, 444
MspI CCGG 2 cut(s) 143, 362
MspR9I CCNGG 2 cut(s) 54, 144
MvaI CCWGG 1 cut(s) 54
MwoI GCNNNNNNNGC 1 cut(s) 314
NciI CCSGG 1 cut(s) 144
NcoI CCATGG 1 cut(s) 249
NdeI CATATG 1 cut(s) 38
NdeII GATC 2 cut(s) 424, 500
NlaIII CATG 4 cut(s) 253, 385, 431, 507
NmuCI GTSAC 1 cut(s) 158
NspI RCATGY 1 cut(s) 385
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PfeI GAWTC 2 cut(s) 189, 398
PfoI TCCNGGA 1 cut(s) 52
PkrI GCNGC 1 cut(s) 13
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
Psp6I CCWGG 1 cut(s) 52
PspGI CCWGG 1 cut(s) 52
PspPI GGNCC 2 cut(s) 145, 253
RsaI GTAC 2 cut(s) 488, 544
RsaNI GTAC 2 cut(s) 487, 543
SaqAI TTAA 2 cut(s) 387, 444
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 2 cut(s) 424, 500
Sau96I GGNCC 2 cut(s) 145, 253
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 2 cut(s) 54, 144
SduI GDGCHC 2 cut(s) 28, 319
SetI ASST 4 cut(s) 129, 258, 293, 488
SfaNI GCATC 2 cut(s) 128, 148
SinI GGWCC 1 cut(s) 253
SmlI CTYRAG 2 cut(s) 43, 320
SmoI CTYRAG 2 cut(s) 43, 320
Sse9I AATT 4 cut(s) 343, 432, 445, 457
SsiI CCGC 2 cut(s) 215, 229
StyD4I CCNGG 2 cut(s) 52, 142
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 1 cut(s) 329
TaqI TCGA 1 cut(s) 219
TasI AATT 4 cut(s) 343, 432, 445, 457
TatI WGTACW 1 cut(s) 542
TfiI GAWTC 2 cut(s) 189, 398
Tru1I TTAA 2 cut(s) 387, 444
Tru9I TTAA 2 cut(s) 387, 444
TscAI CASTG 2 cut(s) 208, 273
TseFI GTSAC 1 cut(s) 158
TseI GCWGC 1 cut(s) 11
Tsp45I GTSAC 1 cut(s) 158
TspDTI ATGAA 2 cut(s) 435, 546
TspGWI ACGGA 1 cut(s) 99
TspRI CASTG 2 cut(s) 208, 273
VpaK11BI GGWCC 1 cut(s) 253
XceI RCATGY 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.