MD14G1237300.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015326
Physical Location & Seq
Reverse (-)
31701340 .. 31701888
549 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1237300.v1.1.491

Sequence Viewer

Length: 549 bp
ATGGGGAATGCAGCAGTTTCTTGTGCTCCATTAATCATATTCCCAAGCAGTCCAGGAGGAGCAGCAAAAGTTTTAACTTTGGATGGAAGAATGGAAGTTTTCACAAGGCAAGTAAAAGCAGCAGAGCTAATGGTAGAGAATCCAGGCCAGTTTTTGTGCGAGTCTACCACCCTCAAAGTAGGCAACAGAATCCACGGCCTCACCGCCGACAAACCGCTGGAAAGACGGCGGCTTTACTTCCTCCTCCCCATGGACCTCCTCTACTCAGTGCTAACACATGAGGAACTCAGCTCCCTAGCTTACAAGGCTTCAAAGGCACTCAAGAACAGTAGAGGCTTCAAGAATTTCAGCAGGATTTTTCCGGTTTTCGGAGATTTTCGCTTGTTTAATGTTTCGGAATCCAAGAGACTGGATTGTGATGATTATGTAATAATTAGTGTTACTTCAAACAATAATCCAGAGCCAGTAATATTATTGGAGAGGTACTCAAAGCAAAGATCATGGAAGCCTGCCTTGGAGACCATTGATGAGACTCCTTGCACACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

183

Amino Acids

20.43

Weight (kDa)

8.68

Isoelectric Point (pI)

40.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 177 3.6e-33 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015326)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18290
fragaria_vesca FvH4_5g17470
malus_domestica MD06G1228100.v1.1 MD14G1237300.v1.1
prunus_persica Prupe.5G234600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0178221
rosa_laevigata RLG00000005462
rosa_multiflora Rmu_co8411017.1_g000001 Rmu_sc0007855.1_g000004
rosa_roxburghii Rroxscaffold_3G00275070
rosa_rugosa Rorug06G0415100
rosa_samantha Rh7AG014200 Rh7BG013700 Rh7CG013600 Rh7DG013900
rosa_wichuraiana Rw7G001110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 164
AciI CCGC 3 cut(s) 204, 215, 229
AcsI RAATTY 1 cut(s) 343
AfaI GTAC 1 cut(s) 485
AfiI CCNNNNNNNGG 2 cut(s) 250, 368
AgsI TTSAA 3 cut(s) 312, 340, 447
AjnI CCWGG 2 cut(s) 52, 142
AjuI GAANNNNNNNTTGG 2 cut(s) 497, 529
AluBI AGCT 3 cut(s) 127, 291, 299
AluI AGCT 3 cut(s) 127, 291, 299
Alw21I GWGCWC 1 cut(s) 28
Alw26I GTCTC 3 cut(s) 400, 512, 524
AoxI GGCC 2 cut(s) 145, 196
ApeKI GCWGC 3 cut(s) 11, 62, 119
ApoI RAATTY 1 cut(s) 343
AseI ATTAAT 1 cut(s) 32
AspS9I GGNCC 1 cut(s) 253
AsuHPI GGTGA 1 cut(s) 193
AvaII GGWCC 1 cut(s) 253
Bbv12I GWGCWC 1 cut(s) 28
BbvI GCAGC 3 cut(s) 23, 74, 131
BccI CCATC 1 cut(s) 77
BceAI ACGGC 2 cut(s) 211, 242
BciT130I CCWGG 2 cut(s) 54, 144
BcoDI GTCTC 3 cut(s) 400, 512, 524
BfaI CTAG 1 cut(s) 296
BisI GCNGC 4 cut(s) 12, 63, 120, 230
BlsI GCNGC 4 cut(s) 13, 64, 121, 231
Bme1390I CCNGG 2 cut(s) 54, 144
Bme18I GGWCC 1 cut(s) 253
BmgT120I GGNCC 1 cut(s) 253
BmrFI CCNGG 2 cut(s) 54, 144
BplI GAGNNNNNCTC 2 cut(s) 470, 502
BpuEI CTTGAG 1 cut(s) 305
BsaI GGTCTC 1 cut(s) 512
BsaJI CCNNGG 3 cut(s) 193, 249, 513
BsaWI WCCGGW 1 cut(s) 361
Bsc4I CCNNNNNNNGG 2 cut(s) 250, 368
Bse1I ACTGG 3 cut(s) 148, 414, 464
BseBI CCWGG 2 cut(s) 54, 144
BseDI CCNNGG 3 cut(s) 193, 249, 513
BseGI GGATG 1 cut(s) 88
BseLI CCNNNNNNNGG 2 cut(s) 250, 368
BseMII CTCAG 2 cut(s) 279, 301
BseNI ACTGG 3 cut(s) 148, 414, 464
BseRI GAGGAG 3 cut(s) 72, 233, 248
BseXI GCAGC 3 cut(s) 23, 74, 131
BshFI GGCC 2 cut(s) 147, 198
BsiHKAI GWGCWC 1 cut(s) 28
BsiSI CCGG 1 cut(s) 362
BslI CCNNNNNNNGG 2 cut(s) 250, 368
BsmAI GTCTC 3 cut(s) 400, 512, 524
BsmI GAATGC 1 cut(s) 13
BsnI GGCC 2 cut(s) 147, 198
Bso31I GGTCTC 1 cut(s) 512
Bsp1286I GDGCHC 1 cut(s) 28
Bsp143I GATC 1 cut(s) 497
Bsp19I CCATGG 1 cut(s) 249
BspACI CCGC 3 cut(s) 204, 215, 229
BspANI GGCC 2 cut(s) 147, 198
BspCNI CTCAG 2 cut(s) 278, 300
BspTNI GGTCTC 1 cut(s) 512
BsrI ACTGG 3 cut(s) 148, 414, 464
BssECI CCNNGG 3 cut(s) 193, 249, 513
BssMI GATC 1 cut(s) 497
BssT1I CCWWGG 2 cut(s) 249, 513
Bst2UI CCWGG 2 cut(s) 54, 144
Bst4CI ACNGT 1 cut(s) 329
BstC8I GCNNGC 1 cut(s) 510
BstDEI CTNAG 2 cut(s) 265, 287
BstDSI CCRYGG 2 cut(s) 193, 249
BstF5I GGATG 1 cut(s) 88
BstKTI GATC 1 cut(s) 500
BstMAI GTCTC 3 cut(s) 400, 512, 524
BstMBI GATC 1 cut(s) 497
BstMWI GCNNNNNNNGC 2 cut(s) 305, 314
BstNI CCWGG 2 cut(s) 54, 144
BstSCI CCNGG 2 cut(s) 52, 142
BstV1I GCAGC 3 cut(s) 23, 74, 131
BstXI CCANNNNNNTGG 1 cut(s) 409
BsuRI GGCC 2 cut(s) 147, 198
BtgI CCRYGG 2 cut(s) 193, 249
BtsCI GGATG 1 cut(s) 88
BtsIMutI CAGTG 1 cut(s) 273
Cac8I GCNNGC 1 cut(s) 510
Cfr13I GGNCC 1 cut(s) 253
Csp6I GTAC 1 cut(s) 484
CviAII CATG 3 cut(s) 250, 278, 501
CviQI GTAC 1 cut(s) 484
DdeI CTNAG 2 cut(s) 265, 287
DpnI GATC 1 cut(s) 499
DpnII GATC 1 cut(s) 497
Eco130I CCWWGG 2 cut(s) 249, 513
Eco31I GGTCTC 1 cut(s) 512
Eco47I GGWCC 1 cut(s) 253
EcoRII CCWGG 2 cut(s) 52, 142
EcoT14I CCWWGG 2 cut(s) 249, 513
ErhI CCWWGG 2 cut(s) 249, 513
FaeI CATG 3 cut(s) 253, 281, 504
FaiI YATR 5 cut(s) 38, 251, 279, 426, 502
FalI AAGNNNNNCTT 2 cut(s) 497, 529
FatI CATG 3 cut(s) 249, 277, 500
FblI GTMKAC 1 cut(s) 164
Fnu4HI GCNGC 4 cut(s) 12, 63, 120, 230
FokI GGATG 1 cut(s) 95
Fsp4HI GCNGC 4 cut(s) 12, 63, 120, 230
FspBI CTAG 1 cut(s) 296
GluI GCNGC 4 cut(s) 12, 63, 120, 230
HaeIII GGCC 2 cut(s) 147, 198
HapII CCGG 1 cut(s) 362
Hin1II CATG 3 cut(s) 253, 281, 504
HinfI GANTC 5 cut(s) 139, 161, 189, 398, 532
HpaII CCGG 1 cut(s) 362
HphI GGTGA 1 cut(s) 193
Hpy166II GTNNAC 1 cut(s) 165
Hpy188I TCNGA 2 cut(s) 371, 397
Hpy188III TCNNGA 3 cut(s) 322, 340, 458
Hpy8I GTNNAC 1 cut(s) 165
HpyCH4III ACNGT 1 cut(s) 329
HpyCH4V TGCA 2 cut(s) 11, 540
HpyF10VI GCNNNNNNNGC 2 cut(s) 305, 314
HpyF3I CTNAG 2 cut(s) 265, 287
Hsp92II CATG 3 cut(s) 253, 281, 504
Kzo9I GATC 1 cut(s) 497
LmnI GCTCC 3 cut(s) 31, 59, 296
Lsp1109I GCAGC 3 cut(s) 23, 74, 131
MaeI CTAG 1 cut(s) 296
MaeIII GTNAC 1 cut(s) 439
MalI GATC 1 cut(s) 499
MboI GATC 1 cut(s) 497
MboII GAAGA 1 cut(s) 99
MhlI GDGCHC 1 cut(s) 28
MluCI AATT 2 cut(s) 343, 432
MlyI GAGTC 2 cut(s) 170, 526
MseI TTAA 3 cut(s) 32, 74, 387
MspA1I CMGCKG 1 cut(s) 217
MspI CCGG 1 cut(s) 362
MspR9I CCNGG 2 cut(s) 54, 144
Mva1269I GAATGC 1 cut(s) 13
MvaI CCWGG 2 cut(s) 54, 144
MwoI GCNNNNNNNGC 2 cut(s) 305, 314
NcoI CCATGG 1 cut(s) 249
NdeII GATC 1 cut(s) 497
NlaIII CATG 3 cut(s) 253, 281, 504
PctI GAATGC 1 cut(s) 13
PfeI GAWTC 3 cut(s) 139, 189, 398
PfoI TCCNGGA 1 cut(s) 52
PkrI GCNGC 4 cut(s) 13, 64, 121, 231
PleI GAGTC 2 cut(s) 169, 526
PpsI GAGTC 2 cut(s) 169, 526
PshBI ATTAAT 1 cut(s) 32
Psp6I CCWGG 2 cut(s) 52, 142
PspGI CCWGG 2 cut(s) 52, 142
PspPI GGNCC 1 cut(s) 253
RsaI GTAC 1 cut(s) 485
RsaNI GTAC 1 cut(s) 484
SaqAI TTAA 3 cut(s) 32, 74, 387
SatI GCNGC 4 cut(s) 12, 63, 120, 230
Sau3AI GATC 1 cut(s) 497
Sau96I GGNCC 1 cut(s) 253
SchI GAGTC 2 cut(s) 170, 526
ScrFI CCNGG 2 cut(s) 54, 144
SduI GDGCHC 1 cut(s) 28
SetI ASST 5 cut(s) 129, 258, 293, 301, 485
SinI GGWCC 1 cut(s) 253
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 2 cut(s) 343, 432
SsiI CCGC 3 cut(s) 204, 215, 229
SspI AATATT 1 cut(s) 471
SspMI CTAG 1 cut(s) 296
StyD4I CCNGG 2 cut(s) 52, 142
StyI CCWWGG 2 cut(s) 249, 513
TaaI ACNGT 1 cut(s) 329
TasI AATT 2 cut(s) 343, 432
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 3 cut(s) 139, 189, 398
Tru1I TTAA 3 cut(s) 32, 74, 387
Tru9I TTAA 3 cut(s) 32, 74, 387
TscAI CASTG 1 cut(s) 273
TseI GCWGC 3 cut(s) 11, 62, 119
TspRI CASTG 1 cut(s) 273
VpaK11BI GGWCC 1 cut(s) 253
VspI ATTAAT 1 cut(s) 32
XapI RAATTY 1 cut(s) 343
XmiI GTMKAC 1 cut(s) 164
XspI CTAG 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.