separase
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_5g17590 FvH4_7g11070 |
| rosa_chinensis | RchiOBHm_Chr4g0428231 RchiOBHm_Chr4g0428251 |
| rosa_samantha | Rh4CG301800 |
| rosa_wichuraiana | Rw0G010790 Rw0G010810 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 875 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 351 |
| AccI | GTMKAC | 1 cut(s) | 268 |
| AccII | CGCG | 1 cut(s) | 784 |
| AciI | CCGC | 1 cut(s) | 784 |
| AclWI | GGATC | 1 cut(s) | 101 |
| AcoI | YGGCCR | 1 cut(s) | 342 |
| AcsI | RAATTY | 1 cut(s) | 738 |
| AcuI | CTGAAG | 1 cut(s) | 471 |
| AfaI | GTAC | 2 cut(s) | 643, 686 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 351 |
| AgsI | TTSAA | 4 cut(s) | 119, 361, 398, 843 |
| AjnI | CCWGG | 1 cut(s) | 311 |
| AluBI | AGCT | 4 cut(s) | 164, 596, 862, 879 |
| AluI | AGCT | 4 cut(s) | 164, 596, 862, 879 |
| Alw26I | GTCTC | 1 cut(s) | 496 |
| AlwI | GGATC | 1 cut(s) | 101 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 599 |
| AoxI | GGCC | 2 cut(s) | 151, 342 |
| ApoI | RAATTY | 1 cut(s) | 738 |
| AspA2I | CCTAGG | 1 cut(s) | 758 |
| AspLEI | GCGC | 1 cut(s) | 172 |
| AspS9I | GGNCC | 2 cut(s) | 152, 617 |
| AsuC2I | CCSGG | 1 cut(s) | 500 |
| AsuHPI | GGTGA | 1 cut(s) | 267 |
| AvaII | GGWCC | 1 cut(s) | 617 |
| AvrII | CCTAGG | 1 cut(s) | 758 |
| BalI | TGGCCA | 1 cut(s) | 344 |
| BbsI | GAAGAC | 1 cut(s) | 625 |
| BccI | CCATC | 4 cut(s) | 29, 286, 460, 643 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 171, 205 |
| BciT130I | CCWGG | 1 cut(s) | 313 |
| BclI | TGATCA | 1 cut(s) | 472 |
| BcnI | CCSGG | 1 cut(s) | 500 |
| BcoDI | GTCTC | 1 cut(s) | 496 |
| BfaI | CTAG | 3 cut(s) | 26, 645, 759 |
| BfuAI | ACCTGC | 1 cut(s) | 875 |
| BglII | AGATCT | 1 cut(s) | 478 |
| BlnI | CCTAGG | 1 cut(s) | 758 |
| Bme1390I | CCNGG | 2 cut(s) | 313, 500 |
| Bme18I | GGWCC | 1 cut(s) | 617 |
| BmgT120I | GGNCC | 2 cut(s) | 152, 617 |
| BmrFI | CCNGG | 2 cut(s) | 313, 500 |
| BmsI | GCATC | 2 cut(s) | 201, 731 |
| BpiI | GAAGAC | 1 cut(s) | 625 |
| BpmI | CTGGAG | 2 cut(s) | 375, 621 |
| BpuMI | CCSGG | 1 cut(s) | 500 |
| BsaAI | YACGTR | 1 cut(s) | 750 |
| BsaBI | GATNNNNATC | 1 cut(s) | 477 |
| BsaJI | CCNNGG | 1 cut(s) | 758 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 351 |
| Bse1I | ACTGG | 2 cut(s) | 392, 716 |
| Bse8I | GATNNNNATC | 1 cut(s) | 477 |
| BseBI | CCWGG | 1 cut(s) | 313 |
| BseDI | CCNNGG | 1 cut(s) | 758 |
| BseGI | GGATG | 5 cut(s) | 205, 475, 523, 654, 793 |
| BseJI | GATNNNNATC | 1 cut(s) | 477 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 351 |
| BseMII | CTCAG | 3 cut(s) | 228, 473, 717 |
| BseNI | ACTGG | 2 cut(s) | 392, 716 |
| BseRI | GAGGAG | 3 cut(s) | 333, 530, 812 |
| Bsh1236I | CGCG | 1 cut(s) | 784 |
| BshFI | GGCC | 2 cut(s) | 153, 344 |
| BsiSI | CCGG | 1 cut(s) | 499 |
| BslFI | GGGAC | 1 cut(s) | 454 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 351 |
| BsmAI | GTCTC | 1 cut(s) | 496 |
| BsmFI | GGGAC | 1 cut(s) | 454 |
| BsmI | GAATGC | 2 cut(s) | 404, 698 |
| BsnI | GGCC | 2 cut(s) | 153, 344 |
| Bsp1407I | TGTACA | 1 cut(s) | 684 |
| Bsp143I | GATC | 4 cut(s) | 93, 182, 472, 478 |
| BspACI | CCGC | 1 cut(s) | 784 |
| BspANI | GGCC | 2 cut(s) | 153, 344 |
| BspCNI | CTCAG | 3 cut(s) | 227, 474, 718 |
| BspFNI | CGCG | 1 cut(s) | 784 |
| BspHI | TCATGA | 1 cut(s) | 408 |
| BspMI | ACCTGC | 1 cut(s) | 875 |
| BspPI | GGATC | 1 cut(s) | 101 |
| BsrGI | TGTACA | 1 cut(s) | 684 |
| BsrI | ACTGG | 2 cut(s) | 392, 716 |
| BssECI | CCNNGG | 1 cut(s) | 758 |
| BssMI | GATC | 4 cut(s) | 93, 182, 472, 478 |
| BssT1I | CCWWGG | 1 cut(s) | 758 |
| Bst2UI | CCWGG | 1 cut(s) | 313 |
| Bst4CI | ACNGT | 3 cut(s) | 551, 641, 675 |
| BstAUI | TGTACA | 1 cut(s) | 684 |
| BstBAI | YACGTR | 1 cut(s) | 750 |
| BstC8I | GCNNGC | 4 cut(s) | 245, 698, 720, 877 |
| BstDEI | CTNAG | 3 cut(s) | 214, 482, 726 |
| BstF5I | GGATG | 5 cut(s) | 205, 475, 523, 654, 793 |
| BstFNI | CGCG | 1 cut(s) | 784 |
| BstHHI | GCGC | 1 cut(s) | 172 |
| BstKTI | GATC | 4 cut(s) | 96, 185, 475, 481 |
| BstMAI | GTCTC | 1 cut(s) | 496 |
| BstMBI | GATC | 4 cut(s) | 93, 182, 472, 478 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 876 |
| BstNI | CCWGG | 1 cut(s) | 313 |
| BstNSI | RCATGY | 2 cut(s) | 700, 722 |
| BstSCI | CCNGG | 2 cut(s) | 311, 498 |
| BstSNI | TACGTA | 1 cut(s) | 750 |
| BstUI | CGCG | 1 cut(s) | 784 |
| BstV2I | GAAGAC | 1 cut(s) | 625 |
| BstX2I | RGATCY | 1 cut(s) | 478 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 57 |
| BstYI | RGATCY | 1 cut(s) | 478 |
| BsuRI | GGCC | 2 cut(s) | 153, 344 |
| BtsCI | GGATG | 5 cut(s) | 205, 475, 523, 654, 793 |
| BveI | ACCTGC | 1 cut(s) | 875 |
| Cac8I | GCNNGC | 4 cut(s) | 245, 698, 720, 877 |
| CaiI | CAGNNNCTG | 1 cut(s) | 599 |
| CciI | TCATGA | 1 cut(s) | 408 |
| CfoI | GCGC | 1 cut(s) | 172 |
| Cfr13I | GGNCC | 2 cut(s) | 152, 617 |
| CseI | GACGC | 1 cut(s) | 740 |
| Csp6I | GTAC | 2 cut(s) | 642, 685 |
| CviAII | CATG | 5 cut(s) | 374, 409, 697, 719, 817 |
| CviQI | GTAC | 2 cut(s) | 642, 685 |
| DdeI | CTNAG | 3 cut(s) | 214, 482, 726 |
| DpnI | GATC | 4 cut(s) | 95, 184, 474, 480 |
| DpnII | GATC | 4 cut(s) | 93, 182, 472, 478 |
| EaeI | YGGCCR | 1 cut(s) | 342 |
| Eco105I | TACGTA | 1 cut(s) | 750 |
| Eco130I | CCWWGG | 1 cut(s) | 758 |
| Eco47I | GGWCC | 1 cut(s) | 617 |
| Eco57I | CTGAAG | 1 cut(s) | 471 |
| EcoRII | CCWGG | 1 cut(s) | 311 |
| EcoT14I | CCWWGG | 1 cut(s) | 758 |
| EcoT22I | ATGCAT | 2 cut(s) | 698, 724 |
| ErhI | CCWWGG | 1 cut(s) | 758 |
| FaeI | CATG | 5 cut(s) | 377, 412, 700, 722, 820 |
| FaqI | GGGAC | 1 cut(s) | 454 |
| FatI | CATG | 5 cut(s) | 373, 408, 696, 718, 816 |
| FauI | CCCGC | 1 cut(s) | 777 |
| FauNDI | CATATG | 1 cut(s) | 327 |
| FbaI | TGATCA | 1 cut(s) | 472 |
| FblI | GTMKAC | 1 cut(s) | 268 |
| FokI | GGATG | 5 cut(s) | 212, 482, 510, 661, 800 |
| FspBI | CTAG | 3 cut(s) | 26, 645, 759 |
| GlaI | GCGC | 1 cut(s) | 171 |
| GsuI | CTGGAG | 2 cut(s) | 375, 621 |
| HaeIII | GGCC | 2 cut(s) | 153, 344 |
| HapII | CCGG | 1 cut(s) | 499 |
| HgaI | GACGC | 1 cut(s) | 740 |
| HhaI | GCGC | 1 cut(s) | 172 |
| Hin1II | CATG | 5 cut(s) | 377, 412, 700, 722, 820 |
| Hin6I | GCGC | 1 cut(s) | 170 |
| HinP1I | GCGC | 1 cut(s) | 170 |
| HincII | GTYRAC | 1 cut(s) | 47 |
| HindII | GTYRAC | 1 cut(s) | 47 |
| HindIII | AAGCTT | 1 cut(s) | 877 |
| HinfI | GANTC | 7 cut(s) | 13, 68, 321, 357, 370, 605, 779 |
| HpaII | CCGG | 1 cut(s) | 499 |
| HphI | GGTGA | 1 cut(s) | 267 |
| Hpy166II | GTNNAC | 4 cut(s) | 47, 179, 269, 620 |
| Hpy188I | TCNGA | 9 cut(s) | 98, 105, 217, 421, 451, 465, 483, 727, 856 |
| Hpy188III | TCNNGA | 8 cut(s) | 72, 250, 398, 409, 476, 559, 577, 600 |
| Hpy8I | GTNNAC | 4 cut(s) | 47, 179, 269, 620 |
| Hpy99I | CGWCG | 1 cut(s) | 641 |
| HpyAV | CCTTC | 3 cut(s) | 396, 537, 560 |
| HpyCH4III | ACNGT | 3 cut(s) | 551, 641, 675 |
| HpyCH4IV | ACGT | 1 cut(s) | 749 |
| HpyCH4V | TGCA | 5 cut(s) | 4, 192, 377, 696, 722 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 876 |
| HpyF3I | CTNAG | 3 cut(s) | 214, 482, 726 |
| HpySE526I | ACGT | 1 cut(s) | 749 |
| Hsp92II | CATG | 5 cut(s) | 377, 412, 700, 722, 820 |
| HspAI | GCGC | 1 cut(s) | 170 |
| Ksp22I | TGATCA | 1 cut(s) | 472 |
| Kzo9I | GATC | 4 cut(s) | 93, 182, 472, 478 |
| LmnI | GCTCC | 1 cut(s) | 517 |
| LweI | GCATC | 2 cut(s) | 201, 731 |
| MaeI | CTAG | 3 cut(s) | 26, 645, 759 |
| MaeII | ACGT | 1 cut(s) | 749 |
| MaeIII | GTNAC | 3 cut(s) | 126, 292, 577 |
| MalI | GATC | 4 cut(s) | 95, 184, 474, 480 |
| MboI | GATC | 4 cut(s) | 93, 182, 472, 478 |
| MboII | GAAGA | 4 cut(s) | 22, 625, 680, 784 |
| MfeI | CAATTG | 1 cut(s) | 51 |
| MflI | RGATCY | 1 cut(s) | 478 |
| MlsI | TGGCCA | 1 cut(s) | 344 |
| MluCI | AATT | 5 cut(s) | 51, 334, 347, 656, 738 |
| MluNI | TGGCCA | 1 cut(s) | 344 |
| MlyI | GAGTC | 3 cut(s) | 330, 379, 788 |
| Mox20I | TGGCCA | 1 cut(s) | 344 |
| Mph1103I | ATGCAT | 2 cut(s) | 698, 724 |
| MscI | TGGCCA | 1 cut(s) | 344 |
| MseI | TTAA | 3 cut(s) | 63, 138, 659 |
| Msp20I | TGGCCA | 1 cut(s) | 344 |
| MspI | CCGG | 1 cut(s) | 499 |
| MspR9I | CCNGG | 2 cut(s) | 313, 500 |
| MunI | CAATTG | 1 cut(s) | 51 |
| Mva1269I | GAATGC | 2 cut(s) | 404, 698 |
| MvaI | CCWGG | 1 cut(s) | 313 |
| MvnI | CGCG | 1 cut(s) | 784 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 876 |
| NciI | CCSGG | 1 cut(s) | 500 |
| NdeI | CATATG | 1 cut(s) | 327 |
| NdeII | GATC | 4 cut(s) | 93, 182, 472, 478 |
| NlaIII | CATG | 5 cut(s) | 377, 412, 700, 722, 820 |
| NmuCI | GTSAC | 2 cut(s) | 126, 577 |
| NsiI | ATGCAT | 2 cut(s) | 698, 724 |
| NspI | RCATGY | 2 cut(s) | 700, 722 |
| PaeI | GCATGC | 2 cut(s) | 700, 722 |
| PagI | TCATGA | 1 cut(s) | 408 |
| PctI | GAATGC | 2 cut(s) | 404, 698 |
| PfeI | GAWTC | 4 cut(s) | 13, 68, 357, 605 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 351 |
| PleI | GAGTC | 3 cut(s) | 329, 378, 787 |
| PpsI | GAGTC | 3 cut(s) | 329, 378, 787 |
| Ppu21I | YACGTR | 1 cut(s) | 750 |
| Psp6I | CCWGG | 1 cut(s) | 311 |
| PspGI | CCWGG | 1 cut(s) | 311 |
| PspPI | GGNCC | 2 cut(s) | 152, 617 |
| PstNI | CAGNNNCTG | 1 cut(s) | 599 |
| PsuI | RGATCY | 1 cut(s) | 478 |
| RsaI | GTAC | 2 cut(s) | 643, 686 |
| RsaNI | GTAC | 2 cut(s) | 642, 685 |
| SaqAI | TTAA | 3 cut(s) | 63, 138, 659 |
| Sau3AI | GATC | 4 cut(s) | 93, 182, 472, 478 |
| Sau96I | GGNCC | 2 cut(s) | 152, 617 |
| SchI | GAGTC | 3 cut(s) | 330, 379, 788 |
| ScrFI | CCNGG | 2 cut(s) | 313, 500 |
| SfaNI | GCATC | 2 cut(s) | 201, 731 |
| SinI | GGWCC | 1 cut(s) | 617 |
| SnaBI | TACGTA | 1 cut(s) | 750 |
| SphI | GCATGC | 2 cut(s) | 700, 722 |
| Sse9I | AATT | 5 cut(s) | 51, 334, 347, 656, 738 |
| SsiI | CCGC | 1 cut(s) | 784 |
| SspMI | CTAG | 3 cut(s) | 26, 645, 759 |
| StyD4I | CCNGG | 2 cut(s) | 311, 498 |
| StyI | CCWWGG | 1 cut(s) | 758 |
| TaaI | ACNGT | 3 cut(s) | 551, 641, 675 |
| TaiI | ACGT | 1 cut(s) | 752 |
| TaqI | TCGA | 2 cut(s) | 195, 636 |
| TasI | AATT | 5 cut(s) | 51, 334, 347, 656, 738 |
| TatI | WGTACW | 1 cut(s) | 684 |
| TfiI | GAWTC | 4 cut(s) | 13, 68, 357, 605 |
| Tru1I | TTAA | 3 cut(s) | 63, 138, 659 |
| Tru9I | TTAA | 3 cut(s) | 63, 138, 659 |
| TseFI | GTSAC | 2 cut(s) | 126, 577 |
| Tsp45I | GTSAC | 2 cut(s) | 126, 577 |
| TspDTI | ATGAA | 2 cut(s) | 23, 397 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 351 |
| VpaK11BI | GGWCC | 1 cut(s) | 617 |
| XapI | RAATTY | 1 cut(s) | 738 |
| XceI | RCATGY | 2 cut(s) | 700, 722 |
| XmaJI | CCTAGG | 1 cut(s) | 758 |
| XmiI | GTMKAC | 1 cut(s) | 268 |
| XspI | CTAG | 3 cut(s) | 26, 645, 759 |
| Zsp2I | ATGCAT | 2 cut(s) | 698, 724 |