Rh4CG301800

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0019708
Physical Location & Seq
Forward (+)
56226053 .. 56238363
12311 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG301800.1

Sequence Viewer

Length: 3582 bp
ATGGTGCAGGAGTATATTGTGTATGATGCAGTGCAACTCCAGGCTGCCGAGTACTCTCAGTACTGTGTCATGATGAAAAAAAAAATTCGAGATATACTACATGAAGAAGTAGCAAACAAAGAAATCAGCTGGTTTCTAAAATCTAGAATTTTAGGACTAGACCGTGGAGGGCTCGAAGCCTCTATTGTCTGTTTATCAACAGCAATAGAGTCACTAGTGGGAGAATCTCATGAATTAGCTGTCGCATACTGCTTTCGTGCGTTTTGCACCCAAGAATTGGACCTACAATCACAGAGAGTGCTGGAGGACATCAATGCTGCCATTGGTATTTGGTTAAATATTCCTGTAGAAATGTTGCCCAAAAGTCTATTAATCCTCCTATACCATCTTCTTGATTGGTTATCCATCAAGGGTTGTCAGGAAGTTGAGAGTATATACAAAATAATCATTAGATTATTTGATGTTCATAAGATCGAGAAGAAGTGGTTGGGCATGTTATGGGGATCAAGAAGAACTACTCATGCCTTATGTGCTTCACCTGTTACTGAGGCATTTTTGAAGATGTTATCAGAGCATTATGGTGAAATCTCAAACTCAACTAGTTTTTGGCTGGATCATCTGAAAGCATGCCATCAGTTGGGCGGTATCGCAACAGGCTTTGAACAGTTCTGCACACAATCCAACCAAAACTATGTAGTTCAAAGTTATCACCTCTTGAGGCAAAGTGATAAATATTTTTCTCGCCATACTGCTGCATATTTGTTATATGACTTATCCGAAAGACTTGTGGAGAAGGGAAATATAATTGAGGCTTTGTCATATGCAAACCGAGCTTCCTCTCTGCGTTGGTTTATATTTCAAGAAACATTTCATAAGGAGAAGAATGAAAAAAAAAAGACAATTTGGAATACACTGCAAGTCCGCAGATCTTGTTTTAGTGATGCCTGGTCTTATGATTCTATGGACTTTGACTTGGAAGACTGGTACTGCAGTCCCTGGAAAATTGCTGAATCTTTTCTTGAAAGTCTACTTCAGTTAGGGAAAATTAGAGAACTGAATGGAAATATAACTGGTGCAATAGCTGCTATTGAGATCGGACAGAATATTTGTACAAATAGGTTTCCCATACTGAGACTTACTTTTTCTTCTGTTCTAGGAGGACTTTACTGTAAGCAGCAACGTTTGGACAAGGCAAAATGTAATTTTGAAGAGGATCTTCTAGCGTTCAAGTCTATCAATGAATTTCCTTGCACAAAGTGCAAACTGAGGCTGGAAGCAACTATTTTTCAGAATCCATTACTGTTTTTTGATAACACCAAGACCACCAAGGCCGCATCTTTTGTGAATTTATCTGATGTTAAAGAATGGTCAGCAGTTGCCCGAGCCGCAGTCAATGATTCGGAGTGGAAAAATTATGTGAGTTGCCGTGACACTTCATCTCATTTGCAATGCAATGTGGCCTGTATTTGCAAGGAGCTGGGAGGTTGCTGGCATTGTCTCCATACTGAGGTTATAAAATCGAAGTCGGTGATCAATATTATACATGTGAGGTGGGAACTGGTGCGTAGATGCCTTTCTCTCCGACTCTATGCTGGGTTAGGGATGCTATTGGAGAGTAACAATCAAATTGTTGACATAGATGGTATTTCAGTCCTAGATGCTATTTTGAAAAGCATGTCAATCCTAGTTTGTAGAGATGCATTTTCTGACACGATCTCTAGCAAATTGTTTCTTAAGATAATGGGAAAAGAGATTGCTGGCTATTGTTTACCTGTTGAGTTGGGAGAACTAATATACAACATGTGTTGGTGGTCTCTGCAAGCCCGTTCCGCGGTAACCAGCAGGAGTAAGGCTCTAAGTGATCATGGTCTTTGTCCGGATCTGGAAAGTTTAGCACTTTGGTTGTTATTGGCTTTTATGATAAGCCGGGAGGTTCCTGTACTTTTTCGGAAGGTTTCTAAATTACTTGCCGCACTATTTTCACTCAAGTATGTTTCTGGTCACTCCGACAAGCATCTTTCATTAACATCTCTCCAAAATTCTGGGTCTTGTACTTCAGATCAGTGGGCTGCCTATTTTCATCAAGTTTCATTGGGTTCGGATGTCCCAGGTTCAATTCAAACCATGCTAGCACCAGACAAGATTGATGATCTTGCAAAATTTGTGACAGATTTTTTTGATGGCCTCCCTGCTGGCACTCCAGTAATATGTATAAGTCTGTTTGGAGGTTCTTATACTACCCTCTTACGACAATTGTTATCTTCTCCCTCGAGCCATCAAGCATGGATTTTGGTAACACGTTTGAAATTGAATTGCAAACCTATTACTATGTTTCTGCCAATCTCTGAAATCATACAAGGACAAGGCCGTGACTGGGATGAGGAGGCAGCAAAAAAATGGGTCTGTCGATGGGGTTTGCAAGGTTCCACTGGGATTGATGATATAATTCCAGAGTATCGTATTATAATGGAGGGGCTTCACTTGTCGGAACCGGGAGGAATTAACCTTATATCACTTAATCGTCGCCTAGAGCAATTTGTGAAGAAATTAGAAACTGTATGGTTTGGATCTTCCAAGTATGTGCTTCTTGGGGAATGGTCAAGCTGTAATGTTGTGAAGTGGGCAAATGAGAAGCTATGTAAGAAGCTGGAAAAGAGATTGAAGGACAAGAGACCAACAAATACTGATCTGAAAGTCCTGATTGGGACTTCTACAGATGCCTTGAAGAAGAAAACGTTCTTGTCACAGCTTTACTCCAATGGCATTAAAGAGTTGACTGCATGGGGCTTGAAACAAATACAGGAAGAAGTACAGAAGCTTGAACGCAATAAACCTGATCTGGAAAGAGAACCAATTATCCTAGTGTTGGATCCGGAGATTCAGATGTTACCTGTGGAGCATATGCCAATTTTAAGAGGCCAGGAGGTGTATCGGATGCCCTCAGTTACAAGCATTAGTGAAACCATGGAGAAAAACCAATATCGCAAACGTCAAGTTGGAAGTAGTGAGATTCCATGTGTAGATCCGTATGATGCGTTTGTGCTTATCAATCCAGATACTACTCTGCCACTATGTGGAGACTACTTAGAATGGTCTTTAAAGGAGAGAGGACTCGAGATATACAAAGGAGGAGAAGTTCCACCTTCTGAAAGATTTGCTGAGCTCTTGGGAAACAAAGATATTTACATATATATGGGCCACAATTCCGGGTTAGAATTTGTTCCTTTAAAATTAGTGGAGAAACTAAAGAAGTGTTCTATGGCATGCTTGATGGGGTGCGGGAGCGGTTCTTCAAAACTTAATGGCTGTTTTGCACCAAGTAACACTGCTCTTTCATATTTGCATGCCGGCGCTCCCCTAGTTATTTGTAACTTGTGGGAAGTGCTTACTGGAGAAAATACAAGATTTTTGCAACGGATGATGAATGGATGGTGGATTGAGAGGTCAAATCCACGGGGGCGCCCAATGGCATCATATATGGTTAAAGCTCGTGAAGGTTGCAGTCTAACATATGTAGATGGAGCACAAATATGTTATGGTGTACCCACTGGTATATCAGAAAGAGATATCTCACCCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

1193

Amino Acids

135.22

Weight (kDa)

7.4

Isoelectric Point (pI)

45.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Separin_C PF03568 838 - 1128 7.4e-50 Separin, protease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019708)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17590 FvH4_7g11070
rosa_chinensis RchiOBHm_Chr4g0428231 RchiOBHm_Chr4g0428251
rosa_samantha Rh4CG301800
rosa_wichuraiana Rw0G010790 Rw0G010810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1514, 2465
AccB1I GGYRCC 1 cut(s) 3459
AccB7I CCANNNNNTGG 3 cut(s) 277, 637, 3074
AccBSI CCGCTC 1 cut(s) 3285
AccI GTMKAC 1 cut(s) 1027
AccII CGCG 1 cut(s) 1832
AccIII TCCGGA 2 cut(s) 1876, 2872
AciI CCGC 9 cut(s) 642, 922, 1332, 1386, 1830, 1832, 1971, 3279, 3285
AclI AACGTT 2 cut(s) 1180, 2735
AclWI GGATC 8 cut(s) 511, 621, 1221, 1887, 2575, 2864, 2877, 3017
AcsI RAATTY 7 cut(s) 84, 147, 1241, 1345, 2038, 2159, 3215
AcuI CTGAAG 2 cut(s) 1016, 2040
AcyI GRCGYC 1 cut(s) 3460
AdeI CACNNNGTG 2 cut(s) 1257, 3074
AfaI GTAC 8 cut(s) 53, 62, 986, 1111, 1941, 2053, 2811, 3543
AfiI CCNNNNNNNGG 7 cut(s) 277, 637, 1507, 1831, 2374, 2866, 3074
AflII CTTAAG 1 cut(s) 1733
AflIII ACRYGT 3 cut(s) 1543, 1800, 2297
AhlI ACTAGT 2 cut(s) 214, 599
AjnI CCWGG 5 cut(s) 39, 944, 995, 2107, 2919
AjuI GAANNNNNNNTTGG 2 cut(s) 470, 502
AleI CACNNNNGTG 1 cut(s) 3577
AloI GAACNNNNNNTCC 4 cut(s) 2841, 2873, 3120, 3152
Alw21I GWGCWC 2 cut(s) 3165, 3526
Alw26I GTCTC 5 cut(s) 1126, 1502, 1818, 2665, 3072
AlwI GGATC 8 cut(s) 511, 621, 1221, 1887, 2575, 2864, 2877, 3017
AlwNI CAGNNNCTG 1 cut(s) 996
Ama87I CYCGRG 3 cut(s) 1380, 2269, 3113
Aor13HI TCCGGA 2 cut(s) 1876, 2872
AoxI GGCC 6 cut(s) 1329, 1458, 2182, 2365, 2917, 3196
ApeKI GCWGC 7 cut(s) 44, 317, 752, 1082, 1174, 2069, 2387
ApoI RAATTY 7 cut(s) 84, 147, 1241, 1345, 2038, 2159, 3215
ArsI GACNNNNNNTTYG 2 cut(s) 1089, 1121
AseI ATTAAT 1 cut(s) 371
Asp700I GAANNNNTTC 4 cut(s) 867, 1014, 2735, 3219
AspLEI GCGC 2 cut(s) 3353, 3462
AspS9I GGNCC 2 cut(s) 280, 3196
AsuC2I CCSGG 3 cut(s) 1928, 2493, 3208
AsuHPI GGTGA 5 cut(s) 528, 593, 701, 1540, 3564
AsuNHI GCTAGC 1 cut(s) 2128
AvaI CYCGRG 3 cut(s) 1380, 2269, 3113
AvaII GGWCC 1 cut(s) 280
BaeI ACNNNNGTAYC 2 cut(s) 976, 1009
BamHI GGATCC 1 cut(s) 2869
BanI GGYRCC 1 cut(s) 3459
BanII GRGCYC 2 cut(s) 174, 3165
BauI CACGAG 1 cut(s) 3489
BbsI GAAGAC 1 cut(s) 984
Bbv12I GWGCWC 2 cut(s) 3165, 3526
BbvI GCAGC 7 cut(s) 31, 304, 739, 1069, 1186, 2056, 2399
BceAI ACGGC 2 cut(s) 1410, 2352
BciT130I CCWGG 5 cut(s) 41, 946, 997, 2109, 2921
BclI TGATCA 2 cut(s) 1530, 1861
BcnI CCSGG 3 cut(s) 1928, 2493, 3208
BcoDI GTCTC 5 cut(s) 1126, 1502, 1818, 2665, 3072
BcuI ACTAGT 2 cut(s) 214, 599
BfmI CTRYAG 3 cut(s) 345, 988, 2712
BfoI RGCGCY 2 cut(s) 3354, 3463
BfrI CTTAAG 1 cut(s) 1733
BglII AGATCT 1 cut(s) 926
BlpI GCTNAGC 1 cut(s) 3159
BmcAI AGTACT 2 cut(s) 53, 62
Bme1390I CCNGG 8 cut(s) 41, 946, 997, 1928, 2109, 2493, 2921, 3208
Bme18I GGWCC 1 cut(s) 280
BmeT110I CYCGRG 3 cut(s) 1380, 2269, 3113
BmgT120I GGNCC 2 cut(s) 280, 3196
BmiI GGNNCC 5 cut(s) 1935, 2425, 2490, 2871, 3461
BmrFI CCNGG 8 cut(s) 41, 946, 997, 1928, 2109, 2493, 2921, 3208
BmrI ACTGGG 3 cut(s) 2383, 2439, 3571
BmtI GCTAGC 1 cut(s) 2132
BmuI ACTGGG 3 cut(s) 2383, 2439, 3571
BpiI GAAGAC 1 cut(s) 984
BplI GAGNNNNNCTC 2 cut(s) 1837, 1869
BpmI CTGGAG 4 cut(s) 23, 323, 2184, 3411
Bpu1102I GCTNAGC 1 cut(s) 3159
BpuEI CTTGAG 2 cut(s) 736, 1970
BpuMI CCSGG 3 cut(s) 1928, 2493, 3208
BsaHI GRCGYC 1 cut(s) 3460
BsaI GGTCTC 2 cut(s) 1818, 2665
BsaJI CCNNGG 7 cut(s) 163, 995, 1326, 1830, 2107, 2964, 3452
BsaWI WCCGGW 2 cut(s) 1876, 2872
BsaXI ACNNNNNCTCC 2 cut(s) 3120, 3150
Bsc4I CCNNNNNNNGG 7 cut(s) 277, 637, 1507, 1831, 2374, 2866, 3074
Bse118I RCCGGY 1 cut(s) 3347
Bse1I ACTGG 9 cut(s) 986, 1075, 1563, 2201, 2378, 2434, 3394, 3553, 3577
Bse3DI GCAATG 2 cut(s) 1454, 1459
BseAI TCCGGA 2 cut(s) 1876, 2872
BseBI CCWGG 5 cut(s) 41, 946, 997, 2109, 2921
BseDI CCNNGG 7 cut(s) 163, 995, 1326, 1830, 2107, 2964, 3452
BseGI GGATG 6 cut(s) 1608, 2107, 2383, 2940, 3423, 3434
BseLI CCNNNNNNNGG 7 cut(s) 277, 637, 1507, 1831, 2374, 2866, 3074
BseMI GCAATG 2 cut(s) 1454, 1459
BseMII CTCAG 7 cut(s) 71, 537, 1121, 1256, 1497, 2955, 3150
BseNI ACTGG 9 cut(s) 986, 1075, 1563, 2201, 2378, 2434, 3394, 3553, 3577
BseRI GAGGAG 2 cut(s) 2396, 3144
BseXI GCAGC 7 cut(s) 31, 304, 739, 1069, 1186, 2056, 2399
BseYI CCCAGC 2 cut(s) 1477, 1592
BsgI GTGCAG 2 cut(s) 26, 655
Bsh1236I CGCG 1 cut(s) 1832
BshFI GGCC 6 cut(s) 1331, 1460, 2184, 2367, 2919, 3198
BshNI GGYRCC 1 cut(s) 3459
BsiHKAI GWGCWC 2 cut(s) 3165, 3526
BsiHKCI CYCGRG 3 cut(s) 1380, 2269, 3113
BsiSI CCGG 6 cut(s) 1877, 1927, 2492, 2873, 3207, 3348
BslFI GGGAC 3 cut(s) 978, 2090, 2719
BslI CCNNNNNNNGG 7 cut(s) 277, 637, 1507, 1831, 2374, 2866, 3074
BsmAI GTCTC 5 cut(s) 1126, 1502, 1818, 2665, 3072
BsmFI GGGAC 3 cut(s) 978, 2090, 2719
BsnI GGCC 6 cut(s) 1331, 1460, 2184, 2367, 2919, 3198
Bso31I GGTCTC 2 cut(s) 1818, 2665
BsoBI CYCGRG 3 cut(s) 1380, 2269, 3113
Bsp1286I GDGCHC 3 cut(s) 174, 3165, 3526
Bsp13I TCCGGA 2 cut(s) 1876, 2872
Bsp1407I TGTACA 1 cut(s) 1109
Bsp1720I GCTNAGC 1 cut(s) 3159
Bsp19I CCATGG 1 cut(s) 2964
BspACI CCGC 9 cut(s) 642, 922, 1332, 1386, 1830, 1832, 1971, 3279, 3285
BspANI GGCC 6 cut(s) 1331, 1460, 2184, 2367, 2919, 3198
BspCNI CTCAG 7 cut(s) 70, 538, 1122, 1257, 1498, 2954, 3151
BspEI TCCGGA 2 cut(s) 1876, 2872
BspFNI CGCG 1 cut(s) 1832
BspHI TCATGA 2 cut(s) 69, 229
BspLI GGNNCC 5 cut(s) 1935, 2425, 2490, 2871, 3461
BspMAI CTGCAG 1 cut(s) 992
BspOI GCTAGC 1 cut(s) 2132
BspPI GGATC 8 cut(s) 511, 621, 1221, 1887, 2575, 2864, 2877, 3017
BspT107I GGYRCC 1 cut(s) 3459
BspTI CTTAAG 1 cut(s) 1733
BspTNI GGTCTC 2 cut(s) 1818, 2665
BsrBI CCGCTC 1 cut(s) 3285
BsrDI GCAATG 2 cut(s) 1454, 1459
BsrFI RCCGGY 1 cut(s) 3347
BsrGI TGTACA 1 cut(s) 1109
BsrI ACTGG 9 cut(s) 986, 1075, 1563, 2201, 2378, 2434, 3394, 3553, 3577
BssAI RCCGGY 1 cut(s) 3347
BssECI CCNNGG 7 cut(s) 163, 995, 1326, 1830, 2107, 2964, 3452
BssNI GRCGYC 1 cut(s) 3460
BssSI CACGAG 1 cut(s) 3489
BssT1I CCWWGG 2 cut(s) 1326, 2964
Bst2BI CACGAG 1 cut(s) 3489
Bst2UI CCWGG 5 cut(s) 41, 946, 997, 2109, 2921
Bst4CI ACNGT 6 cut(s) 65, 164, 666, 1169, 1302, 2557
Bst6I CTCTTC 1 cut(s) 1203
BstACI GRCGYC 1 cut(s) 3460
BstAFI CTTAAG 1 cut(s) 1733
BstAPI GCANNNNNTGC 2 cut(s) 1082, 1257
BstAUI TGTACA 1 cut(s) 1109
BstC8I GCNNGC 9 cut(s) 628, 1490, 1759, 1821, 2130, 2194, 3265, 3345, 3349
BstDEI CTNAG 9 cut(s) 57, 546, 1130, 1265, 1506, 1856, 2941, 3085, 3159
BstDSI CCRYGG 4 cut(s) 163, 1830, 2964, 3452
BstEII GGTNACC 1 cut(s) 1834
BstF5I GGATG 6 cut(s) 1608, 2107, 2383, 2940, 3423, 3434
BstFNI CGCG 1 cut(s) 1832
BstH2I RGCGCY 2 cut(s) 3354, 3463
BstHHI GCGC 2 cut(s) 3353, 3462
BstMAI GTCTC 5 cut(s) 1126, 1502, 1818, 2665, 3072
BstMWI GCNNNNNNNGC 6 cut(s) 530, 830, 1082, 1257, 1385, 1829
BstNI CCWGG 5 cut(s) 41, 946, 997, 2109, 2921
BstNSI RCATGY 7 cut(s) 496, 630, 1547, 1678, 1804, 3267, 3347
BstPI GGTNACC 1 cut(s) 1834
BstSCI CCNGG 8 cut(s) 39, 944, 995, 1926, 2107, 2491, 2919, 3206
BstSFI CTRYAG 3 cut(s) 345, 988, 2712
BstUI CGCG 1 cut(s) 1832
BstV1I GCAGC 7 cut(s) 31, 304, 739, 1069, 1186, 2056, 2399
BstV2I GAAGAC 1 cut(s) 984
BstX2I RGATCY 6 cut(s) 926, 1213, 1879, 2567, 2869, 3022
BstXI CCANNNNNNTGG 1 cut(s) 2042
BstYI RGATCY 6 cut(s) 926, 1213, 1879, 2567, 2869, 3022
BsuRI GGCC 6 cut(s) 1331, 1460, 2184, 2367, 2919, 3198
BtgI CCRYGG 4 cut(s) 163, 1830, 2964, 3452
BtsCI GGATG 6 cut(s) 1608, 2107, 2383, 2940, 3423, 3434
BtsI GCAGTG 3 cut(s) 36, 911, 3324
BtsIMutI CAGTG 6 cut(s) 36, 911, 2069, 2427, 3324, 3546
Cac8I GCNNGC 9 cut(s) 628, 1490, 1759, 1821, 2130, 2194, 3265, 3345, 3349
CaiI CAGNNNCTG 1 cut(s) 996
CciI TCATGA 2 cut(s) 69, 229
CfoI GCGC 2 cut(s) 3353, 3462
Cfr10I RCCGGY 1 cut(s) 3347
Cfr13I GGNCC 2 cut(s) 280, 3196
Cfr42I CCGCGG 1 cut(s) 1833
Csp6I GTAC 8 cut(s) 52, 61, 985, 1110, 1940, 2052, 2810, 3542
CspCI CAANNNNNGTGG 2 cut(s) 2416, 2451
CviQI GTAC 8 cut(s) 52, 61, 985, 1110, 1940, 2052, 2810, 3542
DdeI CTNAG 9 cut(s) 57, 546, 1130, 1265, 1506, 1856, 2941, 3085, 3159
DinI GGCGCC 1 cut(s) 3461
DraI TTTAAA 2 cut(s) 3099, 3228
DraIII CACNNNGTG 2 cut(s) 1257, 3074
Eam1104I CTCTTC 1 cut(s) 1203
EarI CTCTTC 1 cut(s) 1203
Ecl136II GAGCTC 1 cut(s) 3163
Eco130I CCWWGG 2 cut(s) 1326, 2964
Eco24I GRGCYC 2 cut(s) 174, 3165
Eco31I GGTCTC 2 cut(s) 1818, 2665
Eco32I GATATC 1 cut(s) 3568
Eco47I GGWCC 1 cut(s) 280
Eco53kI GAGCTC 1 cut(s) 3163
Eco57I CTGAAG 2 cut(s) 1016, 2040
Eco88I CYCGRG 3 cut(s) 1380, 2269, 3113
Eco91I GGTNACC 1 cut(s) 1834
EcoICRI GAGCTC 1 cut(s) 3163
EcoO65I GGTNACC 1 cut(s) 1834
EcoRII CCWGG 5 cut(s) 39, 944, 995, 2107, 2919
EcoRV GATATC 1 cut(s) 3568
EcoT14I CCWWGG 2 cut(s) 1326, 2964
EcoT22I ATGCAT 1 cut(s) 1702
EcoT38I GRGCYC 2 cut(s) 174, 3165
EgeI GGCGCC 1 cut(s) 3461
EheI GGCGCC 1 cut(s) 3461
ErhI CCWWGG 2 cut(s) 1326, 2964
FalI AAGNNNNNCTT 2 cut(s) 1200, 1232
FaqI GGGAC 3 cut(s) 978, 2090, 2719
FauI CCCGC 1 cut(s) 3272
FauNDI CATATG 3 cut(s) 820, 2901, 3511
FbaI TGATCA 2 cut(s) 1530, 1861
FblI GTMKAC 1 cut(s) 1027
FokI GGATG 6 cut(s) 1615, 2114, 2390, 2947, 3430, 3441
FriOI GRGCYC 2 cut(s) 174, 3165
GlaI GCGC 2 cut(s) 3352, 3461
GsaI CCCAGC 2 cut(s) 1481, 1596
GsuI CTGGAG 4 cut(s) 23, 323, 2184, 3411
HaeII RGCGCY 2 cut(s) 3354, 3463
HaeIII GGCC 6 cut(s) 1331, 1460, 2184, 2367, 2919, 3198
HapII CCGG 6 cut(s) 1877, 1927, 2492, 2873, 3207, 3348
HhaI GCGC 2 cut(s) 3353, 3462
Hin1I GRCGYC 1 cut(s) 3460
Hin6I GCGC 2 cut(s) 3351, 3460
HinP1I GCGC 2 cut(s) 3351, 3460
HincII GTYRAC 2 cut(s) 1633, 2775
HindII GTYRAC 2 cut(s) 1633, 2775
HindIII AAGCTT 1 cut(s) 2816
HpaII CCGG 6 cut(s) 1877, 1927, 2492, 2873, 3207, 3348
HphI GGTGA 5 cut(s) 528, 593, 701, 1540, 3564
Hpy166II GTNNAC 5 cut(s) 1028, 1633, 1769, 2775, 3542
Hpy8I GTNNAC 5 cut(s) 1028, 1633, 1769, 2775, 3542
Hpy99I CGWCG 1 cut(s) 2526
HpyAV CCTTC 5 cut(s) 787, 1945, 2656, 3153, 3488
HpyCH4III ACNGT 6 cut(s) 65, 164, 666, 1169, 1302, 2557
HpyCH4IV ACGT 4 cut(s) 1180, 2299, 2735, 2989
HpyF10VI GCNNNNNNNGC 6 cut(s) 530, 830, 1082, 1257, 1385, 1829
HpyF3I CTNAG 9 cut(s) 57, 546, 1130, 1265, 1506, 1856, 2941, 3085, 3159
HpySE526I ACGT 4 cut(s) 1180, 2299, 2735, 2989
Hsp92I GRCGYC 1 cut(s) 3460
HspAI GCGC 2 cut(s) 3351, 3460
KasI GGCGCC 1 cut(s) 3459
Kpn2I TCCGGA 2 cut(s) 1876, 2872
KroI GCCGGC 1 cut(s) 3347
KroNI GCCGGC 1 cut(s) 3349
Ksp22I TGATCA 2 cut(s) 1530, 1861
KspI CCGCGG 1 cut(s) 1833
LmnI GCTCC 5 cut(s) 1474, 2896, 3282, 3358, 3521
Lsp1109I GCAGC 7 cut(s) 31, 304, 739, 1069, 1186, 2056, 2399
MaeII ACGT 4 cut(s) 1180, 2299, 2735, 2989
MbiI CCGCTC 1 cut(s) 3285
MfeI CAATTG 1 cut(s) 2252
MflI RGATCY 6 cut(s) 926, 1213, 1879, 2567, 2869, 3022
MhlI GDGCHC 3 cut(s) 174, 3165, 3526
Mly113I GGCGCC 1 cut(s) 3460
MlyI GAGTC 3 cut(s) 218, 1578, 3105
MmeI TCCRAC 6 cut(s) 705, 1606, 2031, 2466, 2847, 2977
Mph1103I ATGCAT 1 cut(s) 1702
MroI TCCGGA 2 cut(s) 1876, 2872
MroNI GCCGGC 1 cut(s) 3347
MroXI GAANNNNTTC 4 cut(s) 867, 1014, 2735, 3219
MslI CAYNNNNRTG 4 cut(s) 579, 3191, 3529, 3577
MspA1I CMGCKG 2 cut(s) 129, 1832
MspCI CTTAAG 1 cut(s) 1733
MspI CCGG 6 cut(s) 1877, 1927, 2492, 2873, 3207, 3348
MspR9I CCNGG 8 cut(s) 41, 946, 997, 1928, 2109, 2493, 2921, 3208
MunI CAATTG 1 cut(s) 2252
MvaI CCWGG 5 cut(s) 41, 946, 997, 2109, 2921
MvnI CGCG 1 cut(s) 1832
MwoI GCNNNNNNNGC 6 cut(s) 530, 830, 1082, 1257, 1385, 1829
NaeI GCCGGC 1 cut(s) 3349
NarI GGCGCC 1 cut(s) 3460
NciI CCSGG 3 cut(s) 1928, 2493, 3208
NcoI CCATGG 1 cut(s) 2964
NdeI CATATG 3 cut(s) 820, 2901, 3511
NgoMIV GCCGGC 1 cut(s) 3347
NheI GCTAGC 1 cut(s) 2128
NlaIV GGNNCC 5 cut(s) 1935, 2425, 2490, 2871, 3461
NmeAIII GCCGAG 1 cut(s) 73
NmuCI GTSAC 6 cut(s) 210, 1427, 2000, 2164, 2369, 2742
NsiI ATGCAT 1 cut(s) 1702
NspI RCATGY 7 cut(s) 496, 630, 1547, 1678, 1804, 3267, 3347
OliI CACNNNNGTG 1 cut(s) 3577
PaeI GCATGC 3 cut(s) 630, 3267, 3347
PaeR7I CTCGAG 2 cut(s) 2269, 3113
PagI TCATGA 2 cut(s) 69, 229
PciI ACATGT 2 cut(s) 1543, 1800
PdiI GCCGGC 1 cut(s) 3349
PdmI GAANNNNTTC 4 cut(s) 867, 1014, 2735, 3219
PfeI GAWTC 7 cut(s) 224, 956, 1010, 1291, 1397, 2878, 3010
PflMI CCANNNNNTGG 3 cut(s) 277, 637, 3074
PleI GAGTC 3 cut(s) 217, 1578, 3105
PluTI GGCGCC 1 cut(s) 3463
PpsI GAGTC 3 cut(s) 217, 1578, 3105
PscI ACATGT 2 cut(s) 1543, 1800
PshBI ATTAAT 1 cut(s) 371
PsiI TTATAA 2 cut(s) 1514, 2465
Psp124BI GAGCTC 1 cut(s) 3165
Psp1406I AACGTT 2 cut(s) 1180, 2735
Psp6I CCWGG 5 cut(s) 39, 944, 995, 2107, 2919
PspEI GGTNACC 1 cut(s) 1834
PspFI CCCAGC 2 cut(s) 1477, 1592
PspGI CCWGG 5 cut(s) 39, 944, 995, 2107, 2919
PspN4I GGNNCC 5 cut(s) 1935, 2425, 2490, 2871, 3461
PspPI GGNCC 2 cut(s) 280, 3196
PspXI VCTCGAGB 1 cut(s) 2269
PstI CTGCAG 1 cut(s) 992
PstNI CAGNNNCTG 1 cut(s) 996
PsuI RGATCY 6 cut(s) 926, 1213, 1879, 2567, 2869, 3022
PvuII CAGCTG 1 cut(s) 129
RsaI GTAC 8 cut(s) 53, 62, 986, 1111, 1941, 2053, 2811, 3543
RsaNI GTAC 8 cut(s) 52, 61, 985, 1110, 1940, 2052, 2810, 3542
RseI CAYNNNNRTG 4 cut(s) 579, 3191, 3529, 3577
SacI GAGCTC 1 cut(s) 3165
SacII CCGCGG 1 cut(s) 1833
Sau96I GGNCC 2 cut(s) 280, 3196
ScaI AGTACT 2 cut(s) 53, 62
SchI GAGTC 3 cut(s) 218, 1578, 3105
ScrFI CCNGG 8 cut(s) 41, 946, 997, 1928, 2109, 2493, 2921, 3208
SduI GDGCHC 3 cut(s) 174, 3165, 3526
SfcI CTRYAG 3 cut(s) 345, 988, 2712
SfoI GGCGCC 1 cut(s) 3461
Sfr274I CTCGAG 2 cut(s) 2269, 3113
Sfr303I CCGCGG 1 cut(s) 1833
SgrBI CCGCGG 1 cut(s) 1833
SinI GGWCC 1 cut(s) 280
SlaI CTCGAG 2 cut(s) 2269, 3113
SmiMI CAYNNNNRTG 4 cut(s) 579, 3191, 3529, 3577
SmlI CTYRAG 5 cut(s) 715, 1733, 1985, 2269, 3113
SmoI CTYRAG 5 cut(s) 715, 1733, 1985, 2269, 3113
SpeI ACTAGT 2 cut(s) 214, 599
SphI GCATGC 3 cut(s) 630, 3267, 3347
SsiI CCGC 9 cut(s) 642, 922, 1332, 1386, 1830, 1832, 1971, 3279, 3285
SspDI GGCGCC 1 cut(s) 3459
SspI AATATT 4 cut(s) 340, 734, 1105, 1537
SstI GAGCTC 1 cut(s) 3165
StyD4I CCNGG 8 cut(s) 39, 944, 995, 1926, 2107, 2491, 2919, 3206
StyI CCWWGG 2 cut(s) 1326, 2964
TaaI ACNGT 6 cut(s) 65, 164, 666, 1169, 1302, 2557
TaiI ACGT 4 cut(s) 1183, 2302, 2738, 2992
TaqI TCGA 7 cut(s) 88, 174, 474, 1520, 2270, 2407, 3114
TatI WGTACW 6 cut(s) 51, 60, 1109, 1939, 2051, 2809
TauI GCSGC 3 cut(s) 1334, 1388, 1973
TfiI GAWTC 7 cut(s) 224, 956, 1010, 1291, 1397, 2878, 3010
TscAI CASTG 6 cut(s) 36, 918, 2069, 2434, 3331, 3553
TseFI GTSAC 6 cut(s) 210, 1427, 2000, 2164, 2369, 2742
TseI GCWGC 7 cut(s) 44, 317, 752, 1082, 1174, 2069, 2387
Tsp45I GTSAC 6 cut(s) 210, 1427, 2000, 2164, 2369, 2742
TspGWI ACGGA 2 cut(s) 3015, 3430
TspRI CASTG 6 cut(s) 36, 918, 2069, 2434, 3331, 3553
Van91I CCANNNNNTGG 3 cut(s) 277, 637, 3074
Vha464I CTTAAG 1 cut(s) 1733
VpaK11BI GGWCC 1 cut(s) 280
VspI ATTAAT 1 cut(s) 371
XapI RAATTY 7 cut(s) 84, 147, 1241, 1345, 2038, 2159, 3215
XbaI TCTAGA 1 cut(s) 143
XceI RCATGY 7 cut(s) 496, 630, 1547, 1678, 1804, 3267, 3347
XhoI CTCGAG 2 cut(s) 2269, 3113
XmiI GTMKAC 1 cut(s) 1027
XmnI GAANNNNTTC 4 cut(s) 867, 1014, 2735, 3219
ZrmI AGTACT 2 cut(s) 53, 62
Zsp2I ATGCAT 1 cut(s) 1702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.