Rw0G010810

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00465
OG0019708
Physical Location & Seq
Forward (+)
22959 .. 34960
12002 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G010810.1

Sequence Viewer

Length: 3285 bp
ATGGTGCAGGAGTATATAGCGTATGATGAAGTGCAACTCCAGGCCGCCGAGTACTCTCAGTACTGTGTCATGATGAAAAAAAAAATTCGAGATATACTACATGAAGAAGTAGCAAACAAAGAAATCAGCTGGTTTCTAAAATCTAGAATTTTAGGACTAGACAGTGGAGGGCTCGAAGCCTCTATTGTCTGTTTATCAACAGCAATAGAGTCACTAGTGGGAGAATCTCATGAATTAGCTGTCGCATACTGCTTTCGTGCGTTTTGCACCCAAGAATTGGACCTACAATCACAGAGAGTGCTGGAGGACATCAATGCTGCCATTGGTATTTGGTTAAATATTCCTGTAGAAATGTTGCCCAAATGTCTATTAATCCTCCTATACCATCTTCTTGATTGGTTATCCGTCAAGGGTTGTCAGGAAGTTGAGAGTATATACAAAATAATCATTAGATTATTTGATGTTCATAAGATTGAGAAGAAGTGGTTGGGCATGCTATGGGGATCGAGAAGAACTACTCATGCCTTATGTGCTTCACCTGTTACTGAGGCATTTTTGAAGATGTTATCAGAGCATTATGGTGAAATCTCAAACTCAACTAGTTTTTGGCTGGATCATCTGAAAGCATGCCATCAGTTGGGCGGTATCGCAACAGGCTTTGAACAGTTCTGCACACAATCCAACCAAAACGATGTAGTTCAAAGTTATCACCTCTTGAGGCAAAGTGATAAACATTTTTCTCGCCATACTGCCGCATATTTGTTATATGACTTATCCGAAAGACTTGTGGAGAAGGGAAATATAATTGAGGCTTTGTCATATGCAAACCGAGCTTTCTCTATGCGTTGGTTTTTATTTCAAGAAACATTTCATAAGGAGGAGAATGAAAAAAAAAAGACAATTTGGAATACACTGCAAGTCCGCAGATCTTGTTTTAGTGATGCCTGGTCTTATGATTCTATGGACTTTGACTTGGAAGACTGGTACTGCAGTCCCTGGAAAATTGCTGAATCTTTTCTTGAAAGTCTACTTCAGTTAGGGAAAATTAGAGAACTGAATGGAAATATAACTGGTGCAATAGCTGCTATTGAGACCGGACGGAATATTTGTACAAATAGGTTTCCCTTACTGAGACTTACTTTTTCTTCTGTTCTAGGAGGACTTTACTGTAAGCAGCAACGTTTGGACAAGGCAAAAAGTAATTTTGAAGAGGATATTCTAGCGTTCAAGTCTATCAATGAATTTCCTTGCACAAAGTGCAAACTGAGGCTGGAAGCAACTATTTTTCAGAATCCATTACTGTTTTTTGATAACACCAAGACCACCAAGGCCGCATCTTTTGTGAATTTATCTGAAGTTAAAGAATGGTCAGCAGTTGCCCGAGCCGCAGTCAATGATTCGGAGTGGAAAAATTATGTGAGTTGCCGTGACACTTCATCTCATTTGCAATGCAATGAGGCCTGTATTTGCAAGAATCTGGGAGGTTGCTGGCATTGTCTCCATACCGAGGTTATAAAATCGAAGTCGGTGATCAATATTATACATGTGAGGTGGGAGCTGGTGCGTAGATGCCTTTCTCTCCGACTATATGCTGGGTTAGGGATGCTATTAATGGCGAGTAACAATCAAATTCTTGACATAGATGGTATTTCAGTCCTAGATGCTATTTTGAAAAGCATGTCAGTCCTAATAATGGGAAAAGAGAATGCTGGCTATTGTTTACCTGTTGAGTTGGGAGAACTAATATACAACATGTGTTGGTGGTCTCTGCAAGCCCGTTCCGTGGTAACCAGCAAGAGTTATTTATCGGCTCTACGTGATCATGGTCTTTGTCCGGATCTGGAAAGTTTAGCACTTTGGTTGCTATTGGCTTTTATGATAAGCCGGGAGGTTCCTGTACTTTTTCGGAAGGGTTCGGATGTCCCAGGTTCAATTCAAACCATGCTAGCGCCAGACAAGATTGATGATCTTGCAAAATTTGTGACAGATTTTTTTGATGGCCTCCCTGCTGGCACTCCAGTAATATGTATAAGTCTGTTTGGAGGTTCTTATACTACCCTCTTACGACAATTGTTATCTTCTCCCTCGAGCCATCAAGCATGGATTTTGGTAACACGTTTGAAATTGAATTGCAAACCTATTACTATGTTTCTGCCAATCTCTGAAATCATACAAGGACAAGGCCGTGACTGGGATGAGGAGGCAGCTAAAAAATGGGTCTGTCGATGGGGTTTGCAAGGTTCCACTGGGATTGATGATATAATTCCAGAGTATCGTATTATAATGGAGGGGCTTCACTTGTCGGAACCGGGAGGAATTAACCTTATATCTCTGAATCGTCGCCTAGAGCAATTTGTGAAATTGAAGGACAAGAGACCAACAAATACTGATCTGAAAGTCCTGATTGGGACTTCTACAGATGCCTTGGAGAAGAAAACGTTCTTGTCACAGCTTTACTCCAATGGTATTAAAGGGTTGACTGCATGGGGCTTGAAACAATTACAGGAAGAAGTACGGAGGCTTGAACGCATTAAACCTGATCTGGAAAGAGAACCAATTATCCTAGTGTTGGATCCGGAGATTCAGATGTTACCTGTGGAGCATATGCCAATTTTAAGAGGCCAGGAGGTGTATCGGATGCCCTCAGTTACAAGCATTAGTGAAACCATGGAGAAAAACCAATATCGCAAACGTCAAGTTGGAAGTAGTGAGATTCCATGTGTAGATCCGTATGATGCGTTTATGCTCATCAATCCAGATACTACTCTGCCACTATGTGGAGACTACTTAGAATGGTCTTTAAAGGACAGAGGACTCGAGATATACAAAGGAGGAGAAGTTCCACCTTCTGAAAGATTTGCTGAGCTCTTGGGAAACAAAGATATTTACATATATATGGGCCACAATTCCGGGTTAGAATTTGTTCCTTTAAAATTAGTGGAGAAACTAAAGAAGTGTTCTATGACATGCTTGATGGGGTGCGGGAGCGGTTCTTCAAAACTTAATGGCTGTTTTGCACCAAGTAATACTGCTGTTTCATATTTGCATGCCGGCGCTCCCCTAGTTATTTGTAACTTGTGGGAAGTGCTTACTGGAGAAAATACAAGATTTTTACAACGGATGATGAATGGATGGTGGATTGAGAGGTCAAATCCACGGGGGCGCTCAATGGCATCATATATGGTTAAAGCTCGTGAAGGTTGCAGTCTAACATATGTAGATGGAGCACAAATATGTTATGGTGTACCCACTGGTATATCAGAAAGAGATATCTCACCCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

1094

Amino Acids

124.11

Weight (kDa)

6.32

Isoelectric Point (pI)

48.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Separin_C PF03568 783 - 1029 7.7e-45 Separin, protease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019708)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17590 FvH4_7g11070
rosa_chinensis RchiOBHm_Chr4g0428231 RchiOBHm_Chr4g0428251
rosa_samantha Rh4CG301800
rosa_wichuraiana Rw0G010790 Rw0G010810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1514, 2282
AccB7I CCANNNNNTGG 3 cut(s) 277, 637, 2777
AccBSI CCGCTC 1 cut(s) 2988
AccI GTMKAC 1 cut(s) 1027
AccIII TCCGGA 2 cut(s) 1834, 2575
AciI CCGC 8 cut(s) 45, 642, 753, 922, 1332, 1386, 2982, 2988
AclI AACGTT 2 cut(s) 1180, 2438
AclWI GGATC 6 cut(s) 511, 621, 1845, 2567, 2580, 2720
AcsI RAATTY 7 cut(s) 84, 147, 1241, 1345, 1629, 1976, 2918
AcuI CTGAAG 2 cut(s) 1016, 1374
AdeI CACNNNGTG 2 cut(s) 1257, 2777
AfaI GTAC 7 cut(s) 53, 62, 986, 1111, 1899, 2514, 3246
AfiI CCNNNNNNNGG 8 cut(s) 277, 637, 1507, 1693, 1783, 2191, 2569, 2777
AflIII ACRYGT 3 cut(s) 1543, 1752, 2114
AhlI ACTAGT 2 cut(s) 214, 599
AjnI CCWGG 5 cut(s) 39, 944, 995, 1924, 2622
AjuI GAANNNNNNNTTGG 2 cut(s) 470, 502
AleI CACNNNNGTG 1 cut(s) 3280
AloI GAACNNNNNNTCC 4 cut(s) 2544, 2576, 2823, 2855
AluBI AGCT 9 cut(s) 129, 239, 833, 1082, 1558, 2207, 2452, 2866, 3191
AluI AGCT 9 cut(s) 129, 239, 833, 1082, 1558, 2207, 2452, 2866, 3191
Alw21I GWGCWC 2 cut(s) 2868, 3229
Alw26I GTCTC 6 cut(s) 1085, 1126, 1502, 1770, 2368, 2775
AlwI GGATC 6 cut(s) 511, 621, 1845, 2567, 2580, 2720
AlwNI CAGNNNCTG 1 cut(s) 996
Ama87I CYCGRG 3 cut(s) 1380, 2086, 2816
Aor13HI TCCGGA 2 cut(s) 1834, 2575
AoxI GGCC 7 cut(s) 42, 1329, 1458, 1999, 2182, 2620, 2899
ApeKI GCWGC 4 cut(s) 317, 1082, 1174, 2204
ApoI RAATTY 7 cut(s) 84, 147, 1241, 1345, 1629, 1976, 2918
ArsI GACNNNNNNTTYG 2 cut(s) 1089, 1121
AseI ATTAAT 2 cut(s) 371, 1610
Asp700I GAANNNNTTC 4 cut(s) 867, 1014, 2438, 2922
AspLEI GCGC 3 cut(s) 1951, 3056, 3165
AspS9I GGNCC 2 cut(s) 280, 2899
AsuC2I CCSGG 3 cut(s) 1886, 2310, 2911
AsuHPI GGTGA 5 cut(s) 528, 593, 701, 1540, 3267
AsuNHI GCTAGC 1 cut(s) 1945
AvaI CYCGRG 3 cut(s) 1380, 2086, 2816
AvaII GGWCC 1 cut(s) 280
BaeI ACNNNNGTAYC 2 cut(s) 976, 1009
BamHI GGATCC 1 cut(s) 2572
BanII GRGCYC 2 cut(s) 174, 2868
BauI CACGAG 1 cut(s) 3192
BbsI GAAGAC 1 cut(s) 984
Bbv12I GWGCWC 2 cut(s) 2868, 3229
BbvI GCAGC 4 cut(s) 304, 1069, 1186, 2216
BccI CCATC 9 cut(s) 393, 639, 1637, 1991, 2100, 2220, 2968, 3126, 3215
BceAI ACGGC 2 cut(s) 1410, 2169
BciT130I CCWGG 5 cut(s) 41, 946, 997, 1926, 2624
BclI TGATCA 2 cut(s) 1530, 1819
BcnI CCSGG 3 cut(s) 1886, 2310, 2911
BcoDI GTCTC 6 cut(s) 1085, 1126, 1502, 1770, 2368, 2775
BcuI ACTAGT 2 cut(s) 214, 599
BfmI CTRYAG 3 cut(s) 345, 988, 2415
BfoI RGCGCY 3 cut(s) 1952, 3057, 3166
BglII AGATCT 1 cut(s) 926
BisI GCNGC 8 cut(s) 45, 318, 753, 1083, 1175, 1332, 1386, 2205
BlpI GCTNAGC 1 cut(s) 2862
BlsI GCNGC 8 cut(s) 46, 319, 754, 1084, 1176, 1333, 1387, 2206
BmcAI AGTACT 2 cut(s) 53, 62
Bme1390I CCNGG 8 cut(s) 41, 946, 997, 1886, 1926, 2310, 2624, 2911
Bme18I GGWCC 1 cut(s) 280
BmeT110I CYCGRG 3 cut(s) 1380, 2086, 2816
BmgT120I GGNCC 2 cut(s) 280, 2899
BmiI GGNNCC 4 cut(s) 1893, 2242, 2307, 2574
BmrFI CCNGG 8 cut(s) 41, 946, 997, 1886, 1926, 2310, 2624, 2911
BmrI ACTGGG 3 cut(s) 2200, 2256, 3274
BmsI GCATC 9 cut(s) 931, 1343, 1559, 1593, 1651, 2410, 2628, 2725, 3182
BmtI GCTAGC 1 cut(s) 1949
BmuI ACTGGG 3 cut(s) 2200, 2256, 3274
BpiI GAAGAC 1 cut(s) 984
BpmI CTGGAG 4 cut(s) 23, 323, 2001, 3114
Bpu1102I GCTNAGC 1 cut(s) 2862
BpuEI CTTGAG 1 cut(s) 736
BpuMI CCSGG 3 cut(s) 1886, 2310, 2911
BsaAI YACGTR 1 cut(s) 1817
BsaI GGTCTC 3 cut(s) 1085, 1770, 2368
BsaJI CCNNGG 8 cut(s) 995, 1326, 1506, 1782, 1924, 2424, 2667, 3155
BsaWI WCCGGW 3 cut(s) 1094, 1834, 2575
BsaXI ACNNNNNCTCC 2 cut(s) 2823, 2853
Bsc4I CCNNNNNNNGG 8 cut(s) 277, 637, 1507, 1693, 1783, 2191, 2569, 2777
Bse118I RCCGGY 1 cut(s) 3050
Bse1I ACTGG 8 cut(s) 986, 1075, 2018, 2195, 2251, 3097, 3256, 3280
Bse3DI GCAATG 2 cut(s) 1454, 1459
BseAI TCCGGA 2 cut(s) 1834, 2575
BseBI CCWGG 5 cut(s) 41, 946, 997, 1926, 2624
BseDI CCNNGG 8 cut(s) 995, 1326, 1506, 1782, 1924, 2424, 2667, 3155
BseGI GGATG 6 cut(s) 1608, 1924, 2200, 2643, 3126, 3137
BseLI CCNNNNNNNGG 8 cut(s) 277, 637, 1507, 1693, 1783, 2191, 2569, 2777
BseMI GCAATG 2 cut(s) 1454, 1459
BseMII CTCAG 6 cut(s) 71, 537, 1121, 1256, 2658, 2853
BseNI ACTGG 8 cut(s) 986, 1075, 2018, 2195, 2251, 3097, 3256, 3280
BseRI GAGGAG 3 cut(s) 893, 2213, 2847
BseXI GCAGC 4 cut(s) 304, 1069, 1186, 2216
BseYI CCCAGC 1 cut(s) 1592
BsgI GTGCAG 2 cut(s) 26, 655
BshFI GGCC 7 cut(s) 44, 1331, 1460, 2001, 2184, 2622, 2901
BsiHKAI GWGCWC 2 cut(s) 2868, 3229
BsiHKCI CYCGRG 3 cut(s) 1380, 2086, 2816
BsiSI CCGG 7 cut(s) 1095, 1835, 1885, 2309, 2576, 2910, 3051
BslFI GGGAC 3 cut(s) 978, 1907, 2422
BslI CCNNNNNNNGG 8 cut(s) 277, 637, 1507, 1693, 1783, 2191, 2569, 2777
BsmAI GTCTC 6 cut(s) 1085, 1126, 1502, 1770, 2368, 2775
BsmFI GGGAC 3 cut(s) 978, 1907, 2422
BsmI GAATGC 1 cut(s) 1711
BsnI GGCC 7 cut(s) 44, 1331, 1460, 2001, 2184, 2622, 2901
Bso31I GGTCTC 3 cut(s) 1085, 1770, 2368
BsoBI CYCGRG 3 cut(s) 1380, 2086, 2816
Bsp1286I GDGCHC 3 cut(s) 174, 2868, 3229
Bsp13I TCCGGA 2 cut(s) 1834, 2575
Bsp1407I TGTACA 1 cut(s) 1109
Bsp1720I GCTNAGC 1 cut(s) 2862
Bsp19I CCATGG 1 cut(s) 2667
BspACI CCGC 8 cut(s) 45, 642, 753, 922, 1332, 1386, 2982, 2988
BspANI GGCC 7 cut(s) 44, 1331, 1460, 2001, 2184, 2622, 2901
BspCNI CTCAG 6 cut(s) 70, 538, 1122, 1257, 2657, 2854
BspEI TCCGGA 2 cut(s) 1834, 2575
BspHI TCATGA 2 cut(s) 69, 229
BspLI GGNNCC 4 cut(s) 1893, 2242, 2307, 2574
BspMAI CTGCAG 1 cut(s) 992
BspOI GCTAGC 1 cut(s) 1949
BspPI GGATC 6 cut(s) 511, 621, 1845, 2567, 2580, 2720
BspTNI GGTCTC 3 cut(s) 1085, 1770, 2368
BsrBI CCGCTC 1 cut(s) 2988
BsrDI GCAATG 2 cut(s) 1454, 1459
BsrFI RCCGGY 1 cut(s) 3050
BsrGI TGTACA 1 cut(s) 1109
BsrI ACTGG 8 cut(s) 986, 1075, 2018, 2195, 2251, 3097, 3256, 3280
BssAI RCCGGY 1 cut(s) 3050
BssECI CCNNGG 8 cut(s) 995, 1326, 1506, 1782, 1924, 2424, 2667, 3155
BssSI CACGAG 1 cut(s) 3192
BssT1I CCWWGG 3 cut(s) 1326, 2424, 2667
Bst2BI CACGAG 1 cut(s) 3192
Bst2UI CCWGG 5 cut(s) 41, 946, 997, 1926, 2624
Bst4CI ACNGT 5 cut(s) 65, 164, 666, 1169, 1302
Bst6I CTCTTC 1 cut(s) 1203
BstAPI GCANNNNNTGC 2 cut(s) 1082, 1257
BstAUI TGTACA 1 cut(s) 1109
BstBAI YACGTR 1 cut(s) 1817
BstC8I GCNNGC 9 cut(s) 494, 628, 1490, 1711, 1773, 1947, 2011, 3048, 3052
BstDEI CTNAG 7 cut(s) 57, 546, 1130, 1265, 2644, 2788, 2862
BstDSI CCRYGG 3 cut(s) 1782, 2667, 3155
BstEII GGTNACC 1 cut(s) 1786
BstF5I GGATG 6 cut(s) 1608, 1924, 2200, 2643, 3126, 3137
BstH2I RGCGCY 3 cut(s) 1952, 3057, 3166
BstHHI GCGC 3 cut(s) 1951, 3056, 3165
BstMAI GTCTC 6 cut(s) 1085, 1126, 1502, 1770, 2368, 2775
BstMWI GCNNNNNNNGC 5 cut(s) 530, 830, 1082, 1257, 1385
BstNI CCWGG 5 cut(s) 41, 946, 997, 1926, 2624
BstNSI RCATGY 7 cut(s) 496, 630, 1547, 1681, 1756, 2970, 3050
BstPI GGTNACC 1 cut(s) 1786
BstSCI CCNGG 8 cut(s) 39, 944, 995, 1884, 1924, 2308, 2622, 2909
BstSFI CTRYAG 3 cut(s) 345, 988, 2415
BstV1I GCAGC 4 cut(s) 304, 1069, 1186, 2216
BstV2I GAAGAC 1 cut(s) 984
BstX2I RGATCY 4 cut(s) 926, 1837, 2572, 2725
BstYI RGATCY 4 cut(s) 926, 1837, 2572, 2725
BsuRI GGCC 7 cut(s) 44, 1331, 1460, 2001, 2184, 2622, 2901
BtgI CCRYGG 3 cut(s) 1782, 2667, 3155
BtsCI GGATG 6 cut(s) 1608, 1924, 2200, 2643, 3126, 3137
BtsI GCAGTG 1 cut(s) 911
BtsIMutI CAGTG 4 cut(s) 169, 911, 2244, 3249
Cac8I GCNNGC 9 cut(s) 494, 628, 1490, 1711, 1773, 1947, 2011, 3048, 3052
CaiI CAGNNNCTG 1 cut(s) 996
CciI TCATGA 2 cut(s) 69, 229
CfoI GCGC 3 cut(s) 1951, 3056, 3165
Cfr10I RCCGGY 1 cut(s) 3050
Cfr13I GGNCC 2 cut(s) 280, 2899
Csp6I GTAC 7 cut(s) 52, 61, 985, 1110, 1898, 2513, 3245
CspCI CAANNNNNGTGG 2 cut(s) 2233, 2268
CviQI GTAC 7 cut(s) 52, 61, 985, 1110, 1898, 2513, 3245
DdeI CTNAG 7 cut(s) 57, 546, 1130, 1265, 2644, 2788, 2862
DraI TTTAAA 2 cut(s) 2802, 2931
DraIII CACNNNGTG 2 cut(s) 1257, 2777
Eam1104I CTCTTC 1 cut(s) 1203
EarI CTCTTC 1 cut(s) 1203
Ecl136II GAGCTC 1 cut(s) 2866
Eco130I CCWWGG 3 cut(s) 1326, 2424, 2667
Eco147I AGGCCT 1 cut(s) 1460
Eco24I GRGCYC 2 cut(s) 174, 2868
Eco31I GGTCTC 3 cut(s) 1085, 1770, 2368
Eco32I GATATC 1 cut(s) 3271
Eco47I GGWCC 1 cut(s) 280
Eco53kI GAGCTC 1 cut(s) 2866
Eco57I CTGAAG 2 cut(s) 1016, 1374
Eco88I CYCGRG 3 cut(s) 1380, 2086, 2816
Eco91I GGTNACC 1 cut(s) 1786
EcoICRI GAGCTC 1 cut(s) 2866
EcoO65I GGTNACC 1 cut(s) 1786
EcoRII CCWGG 5 cut(s) 39, 944, 995, 1924, 2622
EcoRV GATATC 1 cut(s) 3271
EcoT14I CCWWGG 3 cut(s) 1326, 2424, 2667
EcoT38I GRGCYC 2 cut(s) 174, 2868
ErhI CCWWGG 3 cut(s) 1326, 2424, 2667
FaqI GGGAC 3 cut(s) 978, 1907, 2422
FauI CCCGC 1 cut(s) 2975
FauNDI CATATG 3 cut(s) 820, 2604, 3214
FbaI TGATCA 2 cut(s) 1530, 1819
FblI GTMKAC 1 cut(s) 1027
Fnu4HI GCNGC 8 cut(s) 45, 318, 753, 1083, 1175, 1332, 1386, 2205
FokI GGATG 6 cut(s) 1615, 1931, 2207, 2650, 3133, 3144
FriOI GRGCYC 2 cut(s) 174, 2868
Fsp4HI GCNGC 8 cut(s) 45, 318, 753, 1083, 1175, 1332, 1386, 2205
GlaI GCGC 3 cut(s) 1950, 3055, 3164
GluI GCNGC 8 cut(s) 45, 318, 753, 1083, 1175, 1332, 1386, 2205
GsaI CCCAGC 1 cut(s) 1596
GsuI CTGGAG 4 cut(s) 23, 323, 2001, 3114
HaeII RGCGCY 3 cut(s) 1952, 3057, 3166
HaeIII GGCC 7 cut(s) 44, 1331, 1460, 2001, 2184, 2622, 2901
HapII CCGG 7 cut(s) 1095, 1835, 1885, 2309, 2576, 2910, 3051
HhaI GCGC 3 cut(s) 1951, 3056, 3165
Hin6I GCGC 3 cut(s) 1949, 3054, 3163
HinP1I GCGC 3 cut(s) 1949, 3054, 3163
HincII GTYRAC 1 cut(s) 2478
HindII GTYRAC 1 cut(s) 2478
HpaII CCGG 7 cut(s) 1095, 1835, 1885, 2309, 2576, 2910, 3051
HphI GGTGA 5 cut(s) 528, 593, 701, 1540, 3267
Hpy166II GTNNAC 4 cut(s) 1028, 1721, 2478, 3245
Hpy8I GTNNAC 4 cut(s) 1028, 1721, 2478, 3245
Hpy99I CGWCG 1 cut(s) 2343
HpyAV CCTTC 5 cut(s) 787, 1903, 2359, 2856, 3191
HpyCH4III ACNGT 5 cut(s) 65, 164, 666, 1169, 1302
HpyCH4IV ACGT 5 cut(s) 1180, 1816, 2116, 2438, 2692
HpyF10VI GCNNNNNNNGC 5 cut(s) 530, 830, 1082, 1257, 1385
HpyF3I CTNAG 7 cut(s) 57, 546, 1130, 1265, 2644, 2788, 2862
HpySE526I ACGT 5 cut(s) 1180, 1816, 2116, 2438, 2692
HspAI GCGC 3 cut(s) 1949, 3054, 3163
Kpn2I TCCGGA 2 cut(s) 1834, 2575
KroI GCCGGC 1 cut(s) 3050
KroNI GCCGGC 1 cut(s) 3052
Ksp22I TGATCA 2 cut(s) 1530, 1819
LmnI GCTCC 5 cut(s) 1555, 2599, 2985, 3061, 3224
Lsp1109I GCAGC 4 cut(s) 304, 1069, 1186, 2216
LweI GCATC 9 cut(s) 931, 1343, 1559, 1593, 1651, 2410, 2628, 2725, 3182
MaeII ACGT 5 cut(s) 1180, 1816, 2116, 2438, 2692
MbiI CCGCTC 1 cut(s) 2988
MfeI CAATTG 1 cut(s) 2069
MflI RGATCY 4 cut(s) 926, 1837, 2572, 2725
MhlI GDGCHC 3 cut(s) 174, 2868, 3229
MlyI GAGTC 2 cut(s) 218, 2808
MmeI TCCRAC 5 cut(s) 705, 1606, 2283, 2550, 2680
MroI TCCGGA 2 cut(s) 1834, 2575
MroNI GCCGGC 1 cut(s) 3050
MroXI GAANNNNTTC 4 cut(s) 867, 1014, 2438, 2922
MslI CAYNNNNRTG 4 cut(s) 579, 2894, 3232, 3280
MspA1I CMGCKG 1 cut(s) 129
MspI CCGG 7 cut(s) 1095, 1835, 1885, 2309, 2576, 2910, 3051
MspR9I CCNGG 8 cut(s) 41, 946, 997, 1886, 1926, 2310, 2624, 2911
MunI CAATTG 1 cut(s) 2069
Mva1269I GAATGC 1 cut(s) 1711
MvaI CCWGG 5 cut(s) 41, 946, 997, 1926, 2624
MwoI GCNNNNNNNGC 5 cut(s) 530, 830, 1082, 1257, 1385
NaeI GCCGGC 1 cut(s) 3052
NciI CCSGG 3 cut(s) 1886, 2310, 2911
NcoI CCATGG 1 cut(s) 2667
NdeI CATATG 3 cut(s) 820, 2604, 3214
NgoMIV GCCGGC 1 cut(s) 3050
NheI GCTAGC 1 cut(s) 1945
NlaIV GGNNCC 4 cut(s) 1893, 2242, 2307, 2574
NmeAIII GCCGAG 1 cut(s) 73
NmuCI GTSAC 5 cut(s) 210, 1427, 1981, 2186, 2445
NspI RCATGY 7 cut(s) 496, 630, 1547, 1681, 1756, 2970, 3050
OliI CACNNNNGTG 1 cut(s) 3280
PaeI GCATGC 3 cut(s) 496, 630, 3050
PaeR7I CTCGAG 2 cut(s) 2086, 2816
PagI TCATGA 2 cut(s) 69, 229
PceI AGGCCT 1 cut(s) 1460
PciI ACATGT 2 cut(s) 1543, 1752
PctI GAATGC 1 cut(s) 1711
PdiI GCCGGC 1 cut(s) 3052
PdmI GAANNNNTTC 4 cut(s) 867, 1014, 2438, 2922
PfeI GAWTC 9 cut(s) 224, 956, 1010, 1291, 1397, 1474, 2335, 2581, 2713
PflMI CCANNNNNTGG 3 cut(s) 277, 637, 2777
PkrI GCNGC 8 cut(s) 46, 319, 754, 1084, 1176, 1333, 1387, 2206
PleI GAGTC 2 cut(s) 217, 2808
PpsI GAGTC 2 cut(s) 217, 2808
Ppu21I YACGTR 1 cut(s) 1817
PscI ACATGT 2 cut(s) 1543, 1752
PshBI ATTAAT 2 cut(s) 371, 1610
PsiI TTATAA 2 cut(s) 1514, 2282
Psp124BI GAGCTC 1 cut(s) 2868
Psp1406I AACGTT 2 cut(s) 1180, 2438
Psp6I CCWGG 5 cut(s) 39, 944, 995, 1924, 2622
PspEI GGTNACC 1 cut(s) 1786
PspFI CCCAGC 1 cut(s) 1592
PspGI CCWGG 5 cut(s) 39, 944, 995, 1924, 2622
PspN4I GGNNCC 4 cut(s) 1893, 2242, 2307, 2574
PspPI GGNCC 2 cut(s) 280, 2899
PspXI VCTCGAGB 1 cut(s) 2086
PstI CTGCAG 1 cut(s) 992
PstNI CAGNNNCTG 1 cut(s) 996
PsuI RGATCY 4 cut(s) 926, 1837, 2572, 2725
PvuII CAGCTG 1 cut(s) 129
RsaI GTAC 7 cut(s) 53, 62, 986, 1111, 1899, 2514, 3246
RsaNI GTAC 7 cut(s) 52, 61, 985, 1110, 1898, 2513, 3245
RseI CAYNNNNRTG 4 cut(s) 579, 2894, 3232, 3280
SacI GAGCTC 1 cut(s) 2868
SatI GCNGC 8 cut(s) 45, 318, 753, 1083, 1175, 1332, 1386, 2205
Sau96I GGNCC 2 cut(s) 280, 2899
ScaI AGTACT 2 cut(s) 53, 62
SchI GAGTC 2 cut(s) 218, 2808
ScrFI CCNGG 8 cut(s) 41, 946, 997, 1886, 1926, 2310, 2624, 2911
SduI GDGCHC 3 cut(s) 174, 2868, 3229
SfaNI GCATC 9 cut(s) 931, 1343, 1559, 1593, 1651, 2410, 2628, 2725, 3182
SfcI CTRYAG 3 cut(s) 345, 988, 2415
Sfr274I CTCGAG 2 cut(s) 2086, 2816
SinI GGWCC 1 cut(s) 280
SlaI CTCGAG 2 cut(s) 2086, 2816
SmiMI CAYNNNNRTG 4 cut(s) 579, 2894, 3232, 3280
SmlI CTYRAG 3 cut(s) 715, 2086, 2816
SmoI CTYRAG 3 cut(s) 715, 2086, 2816
SpeI ACTAGT 2 cut(s) 214, 599
SphI GCATGC 3 cut(s) 496, 630, 3050
SseBI AGGCCT 1 cut(s) 1460
SsiI CCGC 8 cut(s) 45, 642, 753, 922, 1332, 1386, 2982, 2988
SspI AATATT 3 cut(s) 340, 1105, 1537
SstI GAGCTC 1 cut(s) 2868
StuI AGGCCT 1 cut(s) 1460
StyD4I CCNGG 8 cut(s) 39, 944, 995, 1884, 1924, 2308, 2622, 2909
StyI CCWWGG 3 cut(s) 1326, 2424, 2667
TaaI ACNGT 5 cut(s) 65, 164, 666, 1169, 1302
TaiI ACGT 5 cut(s) 1183, 1819, 2119, 2441, 2695
TaqI TCGA 7 cut(s) 88, 174, 506, 1520, 2087, 2224, 2817
TatI WGTACW 4 cut(s) 51, 60, 1109, 1897
TauI GCSGC 4 cut(s) 47, 755, 1334, 1388
TfiI GAWTC 9 cut(s) 224, 956, 1010, 1291, 1397, 1474, 2335, 2581, 2713
TscAI CASTG 4 cut(s) 169, 918, 2251, 3256
TseFI GTSAC 5 cut(s) 210, 1427, 1981, 2186, 2445
TseI GCWGC 4 cut(s) 317, 1082, 1174, 2204
Tsp45I GTSAC 5 cut(s) 210, 1427, 1981, 2186, 2445
TspGWI ACGGA 6 cut(s) 394, 1114, 1771, 2530, 2718, 3133
TspRI CASTG 4 cut(s) 169, 918, 2251, 3256
Van91I CCANNNNNTGG 3 cut(s) 277, 637, 2777
VpaK11BI GGWCC 1 cut(s) 280
VspI ATTAAT 2 cut(s) 371, 1610
XapI RAATTY 7 cut(s) 84, 147, 1241, 1345, 1629, 1976, 2918
XbaI TCTAGA 1 cut(s) 143
XceI RCATGY 7 cut(s) 496, 630, 1547, 1681, 1756, 2970, 3050
XhoI CTCGAG 2 cut(s) 2086, 2816
XmiI GTMKAC 1 cut(s) 1027
XmnI GAANNNNTTC 4 cut(s) 867, 1014, 2438, 2922
ZrmI AGTACT 2 cut(s) 53, 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.