Rw0G010790

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00465
OG0019708
Physical Location & Seq
Forward (+)
1929 .. 8138
6210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G010790.1

Sequence Viewer

Length: 3099 bp
ATGCTATGGGGATCGAGAAGAACTACTCATGCCTTATGTGCTTCACCTGTTACTGAGGCATTTTTGAAGATGTTATCAGAGCATTATGGTGAAATCTCAAACTCAACTAGTTTTTGGCTGGATCATCTGAAAGCATGCCATCAGTTGGGCGGTATCGCAACAGGCTTTGAACAGTTCTGCACACAATCCAACCAAAACGATGTAGTTCAAAGTTATCACCTCTTGAGGCAAAGTGATAAACATTTTTCTCGCCATACTGCCGCATATTTGTTATATGACTTATCCGAAAGACTTGTGGAGAAGGGAAATATAATTGAGGCTTTGTCATATGCAAACCGAGCTTTCTCTATGCGTTGGTTTTTATTTCAAGAAACATTTCATAAGGAGGAGAATGAAAAAAAAAAGACAATTTGGAATACACTGCAAGTCCGCAGATCTTGTTTTAGTGATGCCTGGTCTTATGATTCTATGGACTTTGACTTGGAAGACTGGTACTGCAGTCCCTGGAAAATTGCTGAATCTTTTCTTGAAAGTCTACTTCAGTTAGGGAAAATTAGAGAACTGAATGGAAATATAACTGGTGCAATAGCTGCTATTGAGACCGGACGGAATATTTGTACAAATAGGTTTCCCTTACTGAGACTTACTTTTTCTTCTGTTCTAGGAGGACTTTACTGTAAGCAGCAACGTTTGGACAAGGCAAAAAGTAATTTTGAAGAGGATATTCTAGCGTTCAAGTCTATCAATGAATTTCCTTGCACAAAGTGCAAACTGAGGCTGGAAGCAACTATTTTTCAGAATCCATTACTGTTTTTTGATAACACCAAGACCACCAAGGCCGCATCTTTTGTGAATTTATCTGAAGTTAAAGAATGGTCAGCAGTTGCCCGAGCCGCAGTCAATGATTCGGAGTGGAAAAATTATGTGAGTTGCCGTGACACTTCATCTCATTTGCAATGCAATGAGGCCTGTATTTGCAAGAATCTGGGAGGTTGCTGGCATTGTCTCCATACCGAGGTTATAAAATCGAAGTCGGTGATCAATATTATACATGTGAGGTGGGAGCTGGTGCGTAGATGCCTTTCTCTCCGACTATATGCTGGGTTAGGGATGCTATTAATGGCGAGTAACAATCAAATTCTTGACATAGATGGTATTTCAGTCCTAGATGCTATTTTGAAAAGCATGTCAGTCCTAGTTTGTAGAGATGCATTTTCTGACACGATCTCTAGCAAATTGTTTCTTAAGATAATGGGAAAAGAGAATGCTGGCTATTGTTTACCTGTTGAGTTGGGAGAACTAATATACAACATGTGTTGGTGGTCTCTGCAAGCCCGTTCCGTGGTAACCAGCAAGAGTTATTTATCGGCTCTACGTGATCATGGTCTTTGTCCGGATCTGGAAAGTTTAGCACTTTGGTTGCTATTGGCTTTTATGATAAGCCGGGAGGTTCCTGTACTTTTTCGGAAGGTTTCTAAATTACTTGCCGCACTATTTTCACTCAAGTATGTTTCTGGTCACTCCGACAAGCATCTTTCATTATCATCTCTCCAAAATTTTGGGTCTTGTACTTCAGATCAGTGGGCTGCCTATTTTCATCAAGTTTCATTGGGTTCGGATGTCCCAGGTTCAATTCAAACCATGCTAGCGCCAGACAAGATTGATGATCTTGCAAAATTTGTGACAGATTTTTTTGATGGCCTCCCTGCTGGCACTCCAGTAATATGTATAAGTCTGTTTGGAGGTTCTTATACTACCCTCTTACGACAATTGTTATCTTCTCCCTCGAGCCATCAAGCATGGATTTTGGTAACACGTTTGAAATTGAATTGCAAACCTATTACTATGTTTCTGCCAATCTCTGAAATCATACAAGGACAAGGCCGTGACTGGGATGAGGAGGCAGCTAAAAAATGGGTCTGTCGATGGGGTTTGCAAGGTTCCACTGGGATTGATGATATAATTCCAGAGTATCGTATTATAATGGAGGGGCTTCACTTGTCGGAACCGGGAGGAATTAACCTTATATCTCTGAATCGTCGCCTAGAGCAATTTGTGAAGAAATTAGAAACTGTATGGTTTGGATCTTCCAAGTATGTGCTTCTTGGGGAATGGTCGAGCTGTAATTTTGTGAAGTGGGCAAATGAGAAGCTATGTAAGAAGCTGGAAAAGAGATTGAAGGACAAGAGACCAACAAATACTGATCTGAAAGTCCTGATTGGGACTTCTACAGATGCCTTGGAGAAGAAAACGTTCTTGTCACAGCTTTACTCCAATGGTATTAAAGGGTTGACTGCATGGGGCTTGAAACAATTACAGGAAGAAGTACGGAGGCTTGAACGCATTAAACCTGATCTGGAAAGAGAACCAATTATCCTAGTGTTGGATCCGGAGATTCAGATGTTACCTGTGGAGCATATGCCAATTTTAAGAGGCCAGGAGGTGTATCGGATGCCCTCAGTTACAAGCATTAGTGAAACCATGGAGAAAAACCAATATCGCAAACGTCAAGTTGGAAGTAGTGAGATTCCATGTGTAGATCCGTATGATGCGTTTATGCTCATCAATCCAGATACTACTCTGCCACTATGTGGAGACTACTTAGAATGGTCTTTAAAGGACAGAGGACTCGAGATATACAAAGGAGGAGAAGTTCCACCTTCTGAAAGATTTGCTGAGCTCTTGGGAAACAAAGATATTTACATATATATGGGCCACAATTCCGGGTTAGAATTTGTTCCTTTAAAATTAGTGGAGAAACTAAAGAAGTGTTCTATGACATGCTTGATGGGGTGCGGGAGCGGTTCTTCAAAACTTAATGGCTGTTTTGCACCAAGTAATACTGCTGTTTCATATTTGCATGCCGGCGCTCCCCTAGTTATTTGTAACTTGTGGGAAGTGCTTACTGGAGAAAATACAAGATTTTTACAACGGATGATGAATGGATGGTGGATTGAGAGGTCAAATCCACGGGGGCGCTCAATGGCATCATATATGGTTAAAGCTCGTGAAGGTTGCAGTCTAACATATGTAGATGGAGCACAAATATGTTATGGTGTACCCACTGGTATATCAGAAAGAGATATCTCACCCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

1032

Amino Acids

116.85

Weight (kDa)

8.02

Isoelectric Point (pI)

44.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Separin_C PF03568 677 - 967 2.9e-49 Separin, protease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019708)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17590 FvH4_7g11070
rosa_chinensis RchiOBHm_Chr4g0428231 RchiOBHm_Chr4g0428251
rosa_samantha Rh4CG301800
rosa_wichuraiana Rw0G010790 Rw0G010810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1022, 1982
AccB7I CCANNNNNTGG 2 cut(s) 145, 2591
AccBSI CCGCTC 1 cut(s) 2802
AccI GTMKAC 1 cut(s) 535
AccIII TCCGGA 2 cut(s) 1393, 2389
AciI CCGC 8 cut(s) 150, 261, 430, 840, 894, 1488, 2796, 2802
AclI AACGTT 2 cut(s) 688, 2252
AclWI GGATC 7 cut(s) 19, 129, 1404, 2092, 2381, 2394, 2534
AcsI RAATTY 6 cut(s) 749, 853, 1137, 1555, 1676, 2732
AcuI CTGAAG 3 cut(s) 524, 882, 1557
AdeI CACNNNGTG 2 cut(s) 765, 2591
AfaI GTAC 6 cut(s) 494, 619, 1458, 1570, 2328, 3060
AfiI CCNNNNNNNGG 6 cut(s) 145, 1015, 1342, 1891, 2383, 2591
AflII CTTAAG 1 cut(s) 1244
AflIII ACRYGT 3 cut(s) 1051, 1311, 1814
AhlI ACTAGT 1 cut(s) 107
AjnI CCWGG 4 cut(s) 452, 503, 1624, 2436
AleI CACNNNNGTG 1 cut(s) 3094
AloI GAACNNNNNNTCC 4 cut(s) 2358, 2390, 2637, 2669
Alw21I GWGCWC 2 cut(s) 2682, 3043
Alw26I GTCTC 6 cut(s) 593, 634, 1010, 1329, 2182, 2589
AlwI GGATC 7 cut(s) 19, 129, 1404, 2092, 2381, 2394, 2534
AlwNI CAGNNNCTG 1 cut(s) 504
Ama87I CYCGRG 3 cut(s) 888, 1786, 2630
Aor13HI TCCGGA 2 cut(s) 1393, 2389
AoxI GGCC 6 cut(s) 837, 966, 1699, 1882, 2434, 2713
ApeKI GCWGC 4 cut(s) 590, 682, 1586, 1904
ApoI RAATTY 6 cut(s) 749, 853, 1137, 1555, 1676, 2732
ArsI GACNNNNNNTTYG 2 cut(s) 597, 629
AseI ATTAAT 1 cut(s) 1118
Asp700I GAANNNNTTC 4 cut(s) 375, 522, 2252, 2736
AspLEI GCGC 3 cut(s) 1651, 2870, 2979
AspS9I GGNCC 1 cut(s) 2713
AsuC2I CCSGG 3 cut(s) 1445, 2010, 2725
AsuHPI GGTGA 5 cut(s) 36, 101, 209, 1048, 3081
AsuNHI GCTAGC 1 cut(s) 1645
AvaI CYCGRG 3 cut(s) 888, 1786, 2630
BaeI ACNNNNGTAYC 2 cut(s) 484, 517
BamHI GGATCC 1 cut(s) 2386
BanII GRGCYC 1 cut(s) 2682
BauI CACGAG 1 cut(s) 3006
BbsI GAAGAC 1 cut(s) 492
Bbv12I GWGCWC 2 cut(s) 2682, 3043
BbvI GCAGC 4 cut(s) 577, 694, 1573, 1916
BccI CCATC 8 cut(s) 147, 1145, 1691, 1800, 1920, 2782, 2940, 3029
BceAI ACGGC 2 cut(s) 918, 1869
BciT130I CCWGG 4 cut(s) 454, 505, 1626, 2438
BclI TGATCA 2 cut(s) 1038, 1378
BcnI CCSGG 3 cut(s) 1445, 2010, 2725
BcoDI GTCTC 6 cut(s) 593, 634, 1010, 1329, 2182, 2589
BcuI ACTAGT 1 cut(s) 107
BfmI CTRYAG 2 cut(s) 496, 2229
BfoI RGCGCY 3 cut(s) 1652, 2871, 2980
BfrI CTTAAG 1 cut(s) 1244
BglII AGATCT 1 cut(s) 434
BisI GCNGC 8 cut(s) 261, 591, 683, 840, 894, 1488, 1587, 1905
BlpI GCTNAGC 1 cut(s) 2676
BlsI GCNGC 8 cut(s) 262, 592, 684, 841, 895, 1489, 1588, 1906
Bme1390I CCNGG 7 cut(s) 454, 505, 1445, 1626, 2010, 2438, 2725
BmeT110I CYCGRG 3 cut(s) 888, 1786, 2630
BmgT120I GGNCC 1 cut(s) 2713
BmiI GGNNCC 4 cut(s) 1452, 1942, 2007, 2388
BmrFI CCNGG 7 cut(s) 454, 505, 1445, 1626, 2010, 2438, 2725
BmrI ACTGGG 3 cut(s) 1900, 1956, 3088
BmtI GCTAGC 1 cut(s) 1649
BmuI ACTGGG 3 cut(s) 1900, 1956, 3088
BpiI GAAGAC 1 cut(s) 492
BpmI CTGGAG 2 cut(s) 1701, 2928
Bpu1102I GCTNAGC 1 cut(s) 2676
BpuEI CTTGAG 2 cut(s) 244, 1487
BpuMI CCSGG 3 cut(s) 1445, 2010, 2725
BsaAI YACGTR 1 cut(s) 1376
BsaI GGTCTC 3 cut(s) 593, 1329, 2182
BsaJI CCNNGG 8 cut(s) 503, 834, 1014, 1341, 1624, 2238, 2481, 2969
BsaWI WCCGGW 3 cut(s) 602, 1393, 2389
BsaXI ACNNNNNCTCC 2 cut(s) 2637, 2667
Bsc4I CCNNNNNNNGG 6 cut(s) 145, 1015, 1342, 1891, 2383, 2591
Bse118I RCCGGY 1 cut(s) 2864
Bse1I ACTGG 8 cut(s) 494, 583, 1718, 1895, 1951, 2911, 3070, 3094
Bse3DI GCAATG 2 cut(s) 962, 967
BseAI TCCGGA 2 cut(s) 1393, 2389
BseBI CCWGG 4 cut(s) 454, 505, 1626, 2438
BseDI CCNNGG 8 cut(s) 503, 834, 1014, 1341, 1624, 2238, 2481, 2969
BseGI GGATG 6 cut(s) 1116, 1624, 1900, 2457, 2940, 2951
BseLI CCNNNNNNNGG 6 cut(s) 145, 1015, 1342, 1891, 2383, 2591
BseMI GCAATG 2 cut(s) 962, 967
BseMII CTCAG 5 cut(s) 45, 629, 764, 2472, 2667
BseNI ACTGG 8 cut(s) 494, 583, 1718, 1895, 1951, 2911, 3070, 3094
BseRI GAGGAG 3 cut(s) 401, 1913, 2661
BseXI GCAGC 4 cut(s) 577, 694, 1573, 1916
BseYI CCCAGC 1 cut(s) 1100
BsgI GTGCAG 1 cut(s) 163
BshFI GGCC 6 cut(s) 839, 968, 1701, 1884, 2436, 2715
BsiHKAI GWGCWC 2 cut(s) 2682, 3043
BsiHKCI CYCGRG 3 cut(s) 888, 1786, 2630
BsiSI CCGG 7 cut(s) 603, 1394, 1444, 2009, 2390, 2724, 2865
BslFI GGGAC 3 cut(s) 486, 1607, 2236
BslI CCNNNNNNNGG 6 cut(s) 145, 1015, 1342, 1891, 2383, 2591
BsmAI GTCTC 6 cut(s) 593, 634, 1010, 1329, 2182, 2589
BsmFI GGGAC 3 cut(s) 486, 1607, 2236
BsmI GAATGC 1 cut(s) 1270
BsnI GGCC 6 cut(s) 839, 968, 1701, 1884, 2436, 2715
Bso31I GGTCTC 3 cut(s) 593, 1329, 2182
BsoBI CYCGRG 3 cut(s) 888, 1786, 2630
Bsp1286I GDGCHC 2 cut(s) 2682, 3043
Bsp13I TCCGGA 2 cut(s) 1393, 2389
Bsp1407I TGTACA 1 cut(s) 617
Bsp1720I GCTNAGC 1 cut(s) 2676
Bsp19I CCATGG 1 cut(s) 2481
BspACI CCGC 8 cut(s) 150, 261, 430, 840, 894, 1488, 2796, 2802
BspANI GGCC 6 cut(s) 839, 968, 1701, 1884, 2436, 2715
BspCNI CTCAG 5 cut(s) 46, 630, 765, 2471, 2668
BspEI TCCGGA 2 cut(s) 1393, 2389
BspLI GGNNCC 4 cut(s) 1452, 1942, 2007, 2388
BspMAI CTGCAG 1 cut(s) 500
BspOI GCTAGC 1 cut(s) 1649
BspPI GGATC 7 cut(s) 19, 129, 1404, 2092, 2381, 2394, 2534
BspTI CTTAAG 1 cut(s) 1244
BspTNI GGTCTC 3 cut(s) 593, 1329, 2182
BsrBI CCGCTC 1 cut(s) 2802
BsrDI GCAATG 2 cut(s) 962, 967
BsrFI RCCGGY 1 cut(s) 2864
BsrGI TGTACA 1 cut(s) 617
BsrI ACTGG 8 cut(s) 494, 583, 1718, 1895, 1951, 2911, 3070, 3094
BssAI RCCGGY 1 cut(s) 2864
BssECI CCNNGG 8 cut(s) 503, 834, 1014, 1341, 1624, 2238, 2481, 2969
BssSI CACGAG 1 cut(s) 3006
BssT1I CCWWGG 3 cut(s) 834, 2238, 2481
Bst2BI CACGAG 1 cut(s) 3006
Bst2UI CCWGG 4 cut(s) 454, 505, 1626, 2438
Bst4CI ACNGT 4 cut(s) 174, 677, 810, 2074
Bst6I CTCTTC 1 cut(s) 711
BstAFI CTTAAG 1 cut(s) 1244
BstAPI GCANNNNNTGC 2 cut(s) 590, 765
BstAUI TGTACA 1 cut(s) 617
BstBAI YACGTR 1 cut(s) 1376
BstC8I GCNNGC 8 cut(s) 136, 998, 1270, 1332, 1647, 1711, 2862, 2866
BstDEI CTNAG 6 cut(s) 54, 638, 773, 2458, 2602, 2676
BstDSI CCRYGG 3 cut(s) 1341, 2481, 2969
BstEII GGTNACC 1 cut(s) 1345
BstF5I GGATG 6 cut(s) 1116, 1624, 1900, 2457, 2940, 2951
BstH2I RGCGCY 3 cut(s) 1652, 2871, 2980
BstHHI GCGC 3 cut(s) 1651, 2870, 2979
BstMAI GTCTC 6 cut(s) 593, 634, 1010, 1329, 2182, 2589
BstMWI GCNNNNNNNGC 5 cut(s) 38, 338, 590, 765, 893
BstNI CCWGG 4 cut(s) 454, 505, 1626, 2438
BstNSI RCATGY 6 cut(s) 138, 1055, 1189, 1315, 2784, 2864
BstPI GGTNACC 1 cut(s) 1345
BstSCI CCNGG 7 cut(s) 452, 503, 1443, 1624, 2008, 2436, 2723
BstSFI CTRYAG 2 cut(s) 496, 2229
BstV1I GCAGC 4 cut(s) 577, 694, 1573, 1916
BstV2I GAAGAC 1 cut(s) 492
BstX2I RGATCY 5 cut(s) 434, 1396, 2084, 2386, 2539
BstXI CCANNNNNNTGG 1 cut(s) 1559
BstYI RGATCY 5 cut(s) 434, 1396, 2084, 2386, 2539
BsuRI GGCC 6 cut(s) 839, 968, 1701, 1884, 2436, 2715
BtgI CCRYGG 3 cut(s) 1341, 2481, 2969
BtsCI GGATG 6 cut(s) 1116, 1624, 1900, 2457, 2940, 2951
BtsI GCAGTG 1 cut(s) 419
BtsIMutI CAGTG 4 cut(s) 419, 1586, 1944, 3063
Cac8I GCNNGC 8 cut(s) 136, 998, 1270, 1332, 1647, 1711, 2862, 2866
CaiI CAGNNNCTG 1 cut(s) 504
CfoI GCGC 3 cut(s) 1651, 2870, 2979
Cfr10I RCCGGY 1 cut(s) 2864
Cfr13I GGNCC 1 cut(s) 2713
Csp6I GTAC 6 cut(s) 493, 618, 1457, 1569, 2327, 3059
CspCI CAANNNNNGTGG 2 cut(s) 1933, 1968
CviQI GTAC 6 cut(s) 493, 618, 1457, 1569, 2327, 3059
DdeI CTNAG 6 cut(s) 54, 638, 773, 2458, 2602, 2676
DraI TTTAAA 2 cut(s) 2616, 2745
DraIII CACNNNGTG 2 cut(s) 765, 2591
Eam1104I CTCTTC 1 cut(s) 711
EarI CTCTTC 1 cut(s) 711
Ecl136II GAGCTC 1 cut(s) 2680
Eco130I CCWWGG 3 cut(s) 834, 2238, 2481
Eco147I AGGCCT 1 cut(s) 968
Eco24I GRGCYC 1 cut(s) 2682
Eco31I GGTCTC 3 cut(s) 593, 1329, 2182
Eco32I GATATC 1 cut(s) 3085
Eco53kI GAGCTC 1 cut(s) 2680
Eco57I CTGAAG 3 cut(s) 524, 882, 1557
Eco88I CYCGRG 3 cut(s) 888, 1786, 2630
Eco91I GGTNACC 1 cut(s) 1345
EcoICRI GAGCTC 1 cut(s) 2680
EcoO65I GGTNACC 1 cut(s) 1345
EcoRII CCWGG 4 cut(s) 452, 503, 1624, 2436
EcoRV GATATC 1 cut(s) 3085
EcoT14I CCWWGG 3 cut(s) 834, 2238, 2481
EcoT22I ATGCAT 1 cut(s) 1213
EcoT38I GRGCYC 1 cut(s) 2682
ErhI CCWWGG 3 cut(s) 834, 2238, 2481
FaqI GGGAC 3 cut(s) 486, 1607, 2236
FauI CCCGC 1 cut(s) 2789
FauNDI CATATG 3 cut(s) 328, 2418, 3028
FbaI TGATCA 2 cut(s) 1038, 1378
FblI GTMKAC 1 cut(s) 535
Fnu4HI GCNGC 8 cut(s) 261, 591, 683, 840, 894, 1488, 1587, 1905
FokI GGATG 6 cut(s) 1123, 1631, 1907, 2464, 2947, 2958
FriOI GRGCYC 1 cut(s) 2682
Fsp4HI GCNGC 8 cut(s) 261, 591, 683, 840, 894, 1488, 1587, 1905
GlaI GCGC 3 cut(s) 1650, 2869, 2978
GluI GCNGC 8 cut(s) 261, 591, 683, 840, 894, 1488, 1587, 1905
GsaI CCCAGC 1 cut(s) 1104
GsuI CTGGAG 2 cut(s) 1701, 2928
HaeII RGCGCY 3 cut(s) 1652, 2871, 2980
HaeIII GGCC 6 cut(s) 839, 968, 1701, 1884, 2436, 2715
HapII CCGG 7 cut(s) 603, 1394, 1444, 2009, 2390, 2724, 2865
HhaI GCGC 3 cut(s) 1651, 2870, 2979
Hin6I GCGC 3 cut(s) 1649, 2868, 2977
HinP1I GCGC 3 cut(s) 1649, 2868, 2977
HincII GTYRAC 1 cut(s) 2292
HindII GTYRAC 1 cut(s) 2292
HinfI GANTC 9 cut(s) 464, 518, 799, 905, 982, 2035, 2395, 2527, 2628
HpaII CCGG 7 cut(s) 603, 1394, 1444, 2009, 2390, 2724, 2865
HphI GGTGA 5 cut(s) 36, 101, 209, 1048, 3081
Hpy166II GTNNAC 4 cut(s) 536, 1280, 2292, 3059
Hpy8I GTNNAC 4 cut(s) 536, 1280, 2292, 3059
Hpy99I CGWCG 1 cut(s) 2043
HpyAV CCTTC 5 cut(s) 295, 1462, 2173, 2670, 3005
HpyCH4III ACNGT 4 cut(s) 174, 677, 810, 2074
HpyCH4IV ACGT 5 cut(s) 688, 1375, 1816, 2252, 2506
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 338, 590, 765, 893
HpyF3I CTNAG 6 cut(s) 54, 638, 773, 2458, 2602, 2676
HpySE526I ACGT 5 cut(s) 688, 1375, 1816, 2252, 2506
HspAI GCGC 3 cut(s) 1649, 2868, 2977
Kpn2I TCCGGA 2 cut(s) 1393, 2389
KroI GCCGGC 1 cut(s) 2864
KroNI GCCGGC 1 cut(s) 2866
Ksp22I TGATCA 2 cut(s) 1038, 1378
LmnI GCTCC 5 cut(s) 1063, 2413, 2799, 2875, 3038
Lsp1109I GCAGC 4 cut(s) 577, 694, 1573, 1916
MaeII ACGT 5 cut(s) 688, 1375, 1816, 2252, 2506
MbiI CCGCTC 1 cut(s) 2802
MfeI CAATTG 1 cut(s) 1769
MflI RGATCY 5 cut(s) 434, 1396, 2084, 2386, 2539
MhlI GDGCHC 2 cut(s) 2682, 3043
MlyI GAGTC 1 cut(s) 2622
MmeI TCCRAC 6 cut(s) 213, 1114, 1548, 1983, 2364, 2494
Mph1103I ATGCAT 1 cut(s) 1213
MroI TCCGGA 2 cut(s) 1393, 2389
MroNI GCCGGC 1 cut(s) 2864
MroXI GAANNNNTTC 4 cut(s) 375, 522, 2252, 2736
MslI CAYNNNNRTG 4 cut(s) 87, 2708, 3046, 3094
MspCI CTTAAG 1 cut(s) 1244
MspI CCGG 7 cut(s) 603, 1394, 1444, 2009, 2390, 2724, 2865
MspR9I CCNGG 7 cut(s) 454, 505, 1445, 1626, 2010, 2438, 2725
MunI CAATTG 1 cut(s) 1769
Mva1269I GAATGC 1 cut(s) 1270
MvaI CCWGG 4 cut(s) 454, 505, 1626, 2438
MwoI GCNNNNNNNGC 5 cut(s) 38, 338, 590, 765, 893
NaeI GCCGGC 1 cut(s) 2866
NciI CCSGG 3 cut(s) 1445, 2010, 2725
NcoI CCATGG 1 cut(s) 2481
NdeI CATATG 3 cut(s) 328, 2418, 3028
NgoMIV GCCGGC 1 cut(s) 2864
NheI GCTAGC 1 cut(s) 1645
NlaIV GGNNCC 4 cut(s) 1452, 1942, 2007, 2388
NmuCI GTSAC 5 cut(s) 935, 1517, 1681, 1886, 2259
NsiI ATGCAT 1 cut(s) 1213
NspI RCATGY 6 cut(s) 138, 1055, 1189, 1315, 2784, 2864
OliI CACNNNNGTG 1 cut(s) 3094
PaeI GCATGC 2 cut(s) 138, 2864
PaeR7I CTCGAG 2 cut(s) 1786, 2630
PceI AGGCCT 1 cut(s) 968
PciI ACATGT 2 cut(s) 1051, 1311
PctI GAATGC 1 cut(s) 1270
PdiI GCCGGC 1 cut(s) 2866
PdmI GAANNNNTTC 4 cut(s) 375, 522, 2252, 2736
PfeI GAWTC 8 cut(s) 464, 518, 799, 905, 982, 2035, 2395, 2527
PflMI CCANNNNNTGG 2 cut(s) 145, 2591
PkrI GCNGC 8 cut(s) 262, 592, 684, 841, 895, 1489, 1588, 1906
PleI GAGTC 1 cut(s) 2622
PpsI GAGTC 1 cut(s) 2622
Ppu21I YACGTR 1 cut(s) 1376
PscI ACATGT 2 cut(s) 1051, 1311
PshBI ATTAAT 1 cut(s) 1118
PsiI TTATAA 2 cut(s) 1022, 1982
Psp124BI GAGCTC 1 cut(s) 2682
Psp1406I AACGTT 2 cut(s) 688, 2252
Psp6I CCWGG 4 cut(s) 452, 503, 1624, 2436
PspEI GGTNACC 1 cut(s) 1345
PspFI CCCAGC 1 cut(s) 1100
PspGI CCWGG 4 cut(s) 452, 503, 1624, 2436
PspN4I GGNNCC 4 cut(s) 1452, 1942, 2007, 2388
PspPI GGNCC 1 cut(s) 2713
PspXI VCTCGAGB 1 cut(s) 1786
PstI CTGCAG 1 cut(s) 500
PstNI CAGNNNCTG 1 cut(s) 504
PsuI RGATCY 5 cut(s) 434, 1396, 2084, 2386, 2539
RsaI GTAC 6 cut(s) 494, 619, 1458, 1570, 2328, 3060
RsaNI GTAC 6 cut(s) 493, 618, 1457, 1569, 2327, 3059
RseI CAYNNNNRTG 4 cut(s) 87, 2708, 3046, 3094
SacI GAGCTC 1 cut(s) 2682
SatI GCNGC 8 cut(s) 261, 591, 683, 840, 894, 1488, 1587, 1905
Sau96I GGNCC 1 cut(s) 2713
SchI GAGTC 1 cut(s) 2622
ScrFI CCNGG 7 cut(s) 454, 505, 1445, 1626, 2010, 2438, 2725
SduI GDGCHC 2 cut(s) 2682, 3043
SfcI CTRYAG 2 cut(s) 496, 2229
Sfr274I CTCGAG 2 cut(s) 1786, 2630
SlaI CTCGAG 2 cut(s) 1786, 2630
SmiMI CAYNNNNRTG 4 cut(s) 87, 2708, 3046, 3094
SmlI CTYRAG 5 cut(s) 223, 1244, 1502, 1786, 2630
SmoI CTYRAG 5 cut(s) 223, 1244, 1502, 1786, 2630
SpeI ACTAGT 1 cut(s) 107
SphI GCATGC 2 cut(s) 138, 2864
SseBI AGGCCT 1 cut(s) 968
SsiI CCGC 8 cut(s) 150, 261, 430, 840, 894, 1488, 2796, 2802
SspI AATATT 2 cut(s) 613, 1045
SstI GAGCTC 1 cut(s) 2682
StuI AGGCCT 1 cut(s) 968
StyD4I CCNGG 7 cut(s) 452, 503, 1443, 1624, 2008, 2436, 2723
StyI CCWWGG 3 cut(s) 834, 2238, 2481
TaaI ACNGT 4 cut(s) 174, 677, 810, 2074
TaiI ACGT 5 cut(s) 691, 1378, 1819, 2255, 2509
TaqI TCGA 6 cut(s) 14, 1028, 1787, 1924, 2117, 2631
TatI WGTACW 3 cut(s) 617, 1456, 1568
TauI GCSGC 4 cut(s) 263, 842, 896, 1490
TfiI GAWTC 8 cut(s) 464, 518, 799, 905, 982, 2035, 2395, 2527
TscAI CASTG 4 cut(s) 426, 1586, 1951, 3070
TseFI GTSAC 5 cut(s) 935, 1517, 1681, 1886, 2259
TseI GCWGC 4 cut(s) 590, 682, 1586, 1904
Tsp45I GTSAC 5 cut(s) 935, 1517, 1681, 1886, 2259
TspDTI ATGAA 9 cut(s) 368, 408, 762, 933, 1527, 1586, 1596, 2841, 2954
TspGWI ACGGA 5 cut(s) 622, 1330, 2344, 2532, 2947
TspRI CASTG 4 cut(s) 426, 1586, 1951, 3070
Van91I CCANNNNNTGG 2 cut(s) 145, 2591
Vha464I CTTAAG 1 cut(s) 1244
VspI ATTAAT 1 cut(s) 1118
XapI RAATTY 6 cut(s) 749, 853, 1137, 1555, 1676, 2732
XceI RCATGY 6 cut(s) 138, 1055, 1189, 1315, 2784, 2864
XhoI CTCGAG 2 cut(s) 1786, 2630
XmiI GTMKAC 1 cut(s) 535
XmnI GAANNNNTTC 4 cut(s) 375, 522, 2252, 2736
Zsp2I ATGCAT 1 cut(s) 1213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.